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  • 99
    Thermo Fisher n 2 hydroxyethylpiperazine n 2 ethanesulfonic acid hepes buffer solution
    N 2 Hydroxyethylpiperazine N 2 Ethanesulfonic Acid Hepes Buffer Solution, supplied by Thermo Fisher, used in various techniques. Bioz Stars score: 99/100, based on 4 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/n 2 hydroxyethylpiperazine n 2 ethanesulfonic acid hepes buffer solution/product/Thermo Fisher
    Average 99 stars, based on 4 article reviews
    Price from $9.99 to $1999.99
    n 2 hydroxyethylpiperazine n 2 ethanesulfonic acid hepes buffer solution - by Bioz Stars, 2020-07
    99/100 stars
      Buy from Supplier

    99
    Millipore hepes solution
    Preparation for the eye dissection. (A) A ventilated cart to place the animal cage inside for dark adaptation. (B) A handmade chopper with a razor blade (red arrow) inserted. A micrometer dial on the bottom moves the slicing chamber laterally during the dissection. (C) Grease pens are assembled from a 1 ml syringe and a pipetter tip (~200 µl). Vacuum grease is inserted into the back of the syringe. (D) A dark box for holding retinal preparations. The bottom is filled with water and oxygen is bubbled continuously. When the box is closed, the inside is completely dark and a moist, oxygenated environment is continually provided. Preparations sit on the shelf. (E) A dissecting chamber filled with <t>HEPES</t> solution. PE tubing from an oxygen source continuously delivers oxygen to the retinal tissue. (F) All tools and pipettes are arranged next to the dissecting microscope, with HEPES solution on ice nearby. (G) A slicing chamber to hold retina tissue (white arrow) attached to the <t>Millipore</t> filter paper (green arrow). (H) A plastic coverslip with parallel grease rails (yellow asterisks) and an attached retinal slice (white arrow) with filter paper (green arrow) set into the grease. (I) A micro-pipette filler and a 1 ml pipette for reference.
    Hepes Solution, supplied by Millipore, used in various techniques. Bioz Stars score: 99/100, based on 1168 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/hepes solution/product/Millipore
    Average 99 stars, based on 1168 article reviews
    Price from $9.99 to $1999.99
    hepes solution - by Bioz Stars, 2020-07
    99/100 stars
      Buy from Supplier

    94
    Millipore • hepes solution
    Preparation for the eye dissection. (A) A ventilated cart to place the animal cage inside for dark adaptation. (B) A handmade chopper with a razor blade (red arrow) inserted. A micrometer dial on the bottom moves the slicing chamber laterally during the dissection. (C) Grease pens are assembled from a 1 ml syringe and a pipetter tip (~200 µl). Vacuum grease is inserted into the back of the syringe. (D) A dark box for holding retinal preparations. The bottom is filled with water and oxygen is bubbled continuously. When the box is closed, the inside is completely dark and a moist, oxygenated environment is continually provided. Preparations sit on the shelf. (E) A dissecting chamber filled with <t>HEPES</t> solution. PE tubing from an oxygen source continuously delivers oxygen to the retinal tissue. (F) All tools and pipettes are arranged next to the dissecting microscope, with HEPES solution on ice nearby. (G) A slicing chamber to hold retina tissue (white arrow) attached to the <t>Millipore</t> filter paper (green arrow). (H) A plastic coverslip with parallel grease rails (yellow asterisks) and an attached retinal slice (white arrow) with filter paper (green arrow) set into the grease. (I) A micro-pipette filler and a 1 ml pipette for reference.
    • Hepes Solution, supplied by Millipore, used in various techniques. Bioz Stars score: 94/100, based on 10 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/• hepes solution/product/Millipore
    Average 94 stars, based on 10 article reviews
    Price from $9.99 to $1999.99
    • hepes solution - by Bioz Stars, 2020-07
    94/100 stars
      Buy from Supplier

    99
    Millipore hepes buffer solution
    19 F EXSY spectra of 1.2 mM AM5206 with 0.6 mM <t>HSA</t> in <t>HEPES</t> buffer. Left panel: mixing time t m = 0. Right panel: t m = 30 ms.
    Hepes Buffer Solution, supplied by Millipore, used in various techniques. Bioz Stars score: 99/100, based on 418 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/hepes buffer solution/product/Millipore
    Average 99 stars, based on 418 article reviews
    Price from $9.99 to $1999.99
    hepes buffer solution - by Bioz Stars, 2020-07
    99/100 stars
      Buy from Supplier

    Image Search Results


    Preparation for the eye dissection. (A) A ventilated cart to place the animal cage inside for dark adaptation. (B) A handmade chopper with a razor blade (red arrow) inserted. A micrometer dial on the bottom moves the slicing chamber laterally during the dissection. (C) Grease pens are assembled from a 1 ml syringe and a pipetter tip (~200 µl). Vacuum grease is inserted into the back of the syringe. (D) A dark box for holding retinal preparations. The bottom is filled with water and oxygen is bubbled continuously. When the box is closed, the inside is completely dark and a moist, oxygenated environment is continually provided. Preparations sit on the shelf. (E) A dissecting chamber filled with HEPES solution. PE tubing from an oxygen source continuously delivers oxygen to the retinal tissue. (F) All tools and pipettes are arranged next to the dissecting microscope, with HEPES solution on ice nearby. (G) A slicing chamber to hold retina tissue (white arrow) attached to the Millipore filter paper (green arrow). (H) A plastic coverslip with parallel grease rails (yellow asterisks) and an attached retinal slice (white arrow) with filter paper (green arrow) set into the grease. (I) A micro-pipette filler and a 1 ml pipette for reference.

    Journal: Methods in molecular biology (Clifton, N.J.)

    Article Title: Functional and Morphological Analysis of OFF Bipolar Cells

    doi: 10.1007/978-1-4939-7720-8_15

    Figure Lengend Snippet: Preparation for the eye dissection. (A) A ventilated cart to place the animal cage inside for dark adaptation. (B) A handmade chopper with a razor blade (red arrow) inserted. A micrometer dial on the bottom moves the slicing chamber laterally during the dissection. (C) Grease pens are assembled from a 1 ml syringe and a pipetter tip (~200 µl). Vacuum grease is inserted into the back of the syringe. (D) A dark box for holding retinal preparations. The bottom is filled with water and oxygen is bubbled continuously. When the box is closed, the inside is completely dark and a moist, oxygenated environment is continually provided. Preparations sit on the shelf. (E) A dissecting chamber filled with HEPES solution. PE tubing from an oxygen source continuously delivers oxygen to the retinal tissue. (F) All tools and pipettes are arranged next to the dissecting microscope, with HEPES solution on ice nearby. (G) A slicing chamber to hold retina tissue (white arrow) attached to the Millipore filter paper (green arrow). (H) A plastic coverslip with parallel grease rails (yellow asterisks) and an attached retinal slice (white arrow) with filter paper (green arrow) set into the grease. (I) A micro-pipette filler and a 1 ml pipette for reference.

    Article Snippet: Immediately after the solution is removed, a halved piece of Millipore filter paper is placed on top of the retinal slab and a drop of HEPES solution is placed on top of the Millipore filter paper.

    Techniques: Dissection, Microscopy, Transferring

    Effects of increasing concentrations of betaine (▪), choline chloride (▴), and sucrose (•) on the GP of Laurdan ( X = 0.01) in POPC LUVs. Solutes were dissolved in buffer (5 mM HEPES and 0.1 mM EDTA, at pH 7.4) at concentrations corresponding to given increasing values of osmolarity Π, namely, 0.5, 1, and 2 osm/kg. Changes in fluorophore emission spectra were measured at 25°C. Total lipid concentration was 25 mM. Each data point represents the average of at least three separate measurements with the error bars indicating standard deviation.

    Journal: Biophysical Journal

    Article Title: Comparison of the Effects of Surface Tension and Osmotic Pressure on the Interfacial Hydration of a Fluid Phospholipid Bilayer

    doi:

    Figure Lengend Snippet: Effects of increasing concentrations of betaine (▪), choline chloride (▴), and sucrose (•) on the GP of Laurdan ( X = 0.01) in POPC LUVs. Solutes were dissolved in buffer (5 mM HEPES and 0.1 mM EDTA, at pH 7.4) at concentrations corresponding to given increasing values of osmolarity Π, namely, 0.5, 1, and 2 osm/kg. Changes in fluorophore emission spectra were measured at 25°C. Total lipid concentration was 25 mM. Each data point represents the average of at least three separate measurements with the error bars indicating standard deviation.

    Article Snippet: Choline chloride, EDTA, HEPES, POPC, and sucrose were from Sigma (St. Louis, MO).

    Techniques: Concentration Assay, Standard Deviation

    The Panx1 channels do not contribute to the Ca 2+ signal induced by caffeine but form functional channels at the plasma membrane. (A,B) Indo-1 loaded chromaffin cells were maintained in a Ca 2+ -free Kreb’s Hepes solution (in mM: 140 NaCl, 5.9 KCl, 1.2 MgCl 2 , 2 EGTA, 10 Hepes-NaOH, 10 glucose) and stimulated with a 10 s pulse of 50 mM caffeine. The cytosolic [Ca 2+ ] was monitored by microfluorometry. Data show means ± SEM of basal cytosolic [Ca 2+ ] (A) or the maximum amplitude of the Ca 2+ signals induced by caffeine (B) in control cells ( n = 22), or cells treated with probenecid ( n = 22) or Cbx ( n = 21). ns = non-significant, * p

    Journal: Frontiers in Cellular Neuroscience

    Article Title: Pannexin 1 channels: new actors in the regulation of catecholamine release from adrenal chromaffin cells

    doi: 10.3389/fncel.2014.00270

    Figure Lengend Snippet: The Panx1 channels do not contribute to the Ca 2+ signal induced by caffeine but form functional channels at the plasma membrane. (A,B) Indo-1 loaded chromaffin cells were maintained in a Ca 2+ -free Kreb’s Hepes solution (in mM: 140 NaCl, 5.9 KCl, 1.2 MgCl 2 , 2 EGTA, 10 Hepes-NaOH, 10 glucose) and stimulated with a 10 s pulse of 50 mM caffeine. The cytosolic [Ca 2+ ] was monitored by microfluorometry. Data show means ± SEM of basal cytosolic [Ca 2+ ] (A) or the maximum amplitude of the Ca 2+ signals induced by caffeine (B) in control cells ( n = 22), or cells treated with probenecid ( n = 22) or Cbx ( n = 21). ns = non-significant, * p

    Article Snippet: Then, the cells were incubated for 10 s with a Kreb’s Hepes solution containing 50 μM DAPI (Sigma) in the absence or presence of 50 μM DMPP and the respective Panx1 inhibitors.

    Techniques: Functional Assay

    19 F EXSY spectra of 1.2 mM AM5206 with 0.6 mM HSA in HEPES buffer. Left panel: mixing time t m = 0. Right panel: t m = 30 ms.

    Journal: Journal of pharmaceutics & pharmacology

    Article Title: Targeting the Endocannabinoid System for Neuroprotection: A 19F-NMR Study of a Selective FAAH Inhibitor Binding with an Anandamide Carrier Protein, HSA

    doi:

    Figure Lengend Snippet: 19 F EXSY spectra of 1.2 mM AM5206 with 0.6 mM HSA in HEPES buffer. Left panel: mixing time t m = 0. Right panel: t m = 30 ms.

    Article Snippet: Materials Fatty acid free human serum albumin (HSA) (purity 96%), warfarin sodium, L-tryptophan, oleic acid, and 1 M HEPES buffer solution were purchased from Sigma-Aldrich (St. Louis, MO) and used without further purification.

    Techniques: Mass Spectrometry

    Left: 19 F- NMR titration experiments of AM5206 into 0.6 mM HSA solution. Right: 19 F-NMR spectra by adding the same amounts of AM5206 stock solution (50 mM in DMSO) into 500 μL HEPES buffer. The ligand concentrations listed in each spectrum reflect the soluble fraction of AM5206 as obtained by integrating the 19 F-NMR signals (cf. Figure 4 ).

    Journal: Journal of pharmaceutics & pharmacology

    Article Title: Targeting the Endocannabinoid System for Neuroprotection: A 19F-NMR Study of a Selective FAAH Inhibitor Binding with an Anandamide Carrier Protein, HSA

    doi:

    Figure Lengend Snippet: Left: 19 F- NMR titration experiments of AM5206 into 0.6 mM HSA solution. Right: 19 F-NMR spectra by adding the same amounts of AM5206 stock solution (50 mM in DMSO) into 500 μL HEPES buffer. The ligand concentrations listed in each spectrum reflect the soluble fraction of AM5206 as obtained by integrating the 19 F-NMR signals (cf. Figure 4 ).

    Article Snippet: Materials Fatty acid free human serum albumin (HSA) (purity 96%), warfarin sodium, L-tryptophan, oleic acid, and 1 M HEPES buffer solution were purchased from Sigma-Aldrich (St. Louis, MO) and used without further purification.

    Techniques: Nuclear Magnetic Resonance, Titration

    Integration of 19 F-NMR signals from AM5206 in HEPES buffer with HSA (blue squares) and without HSA (red circles). The two horizontal axes represent the actual amount of AM5206 stock solution (50 mM in DMSO) added and the corresponding amount of AM5206 in the NMR sample (mg/mL). The vertical axis on the right shows the concentration of the soluble portion of AM5206.

    Journal: Journal of pharmaceutics & pharmacology

    Article Title: Targeting the Endocannabinoid System for Neuroprotection: A 19F-NMR Study of a Selective FAAH Inhibitor Binding with an Anandamide Carrier Protein, HSA

    doi:

    Figure Lengend Snippet: Integration of 19 F-NMR signals from AM5206 in HEPES buffer with HSA (blue squares) and without HSA (red circles). The two horizontal axes represent the actual amount of AM5206 stock solution (50 mM in DMSO) added and the corresponding amount of AM5206 in the NMR sample (mg/mL). The vertical axis on the right shows the concentration of the soluble portion of AM5206.

    Article Snippet: Materials Fatty acid free human serum albumin (HSA) (purity 96%), warfarin sodium, L-tryptophan, oleic acid, and 1 M HEPES buffer solution were purchased from Sigma-Aldrich (St. Louis, MO) and used without further purification.

    Techniques: Nuclear Magnetic Resonance, Concentration Assay

    (A) Structure of CN-PPV polymer and the absorption (dashed) and emission (solid) spectra of CN-PPV Pdot (black) and CN-PPV Pdot-streptavidin conjugate (CNPPV-Strep, red) in a 0.1% PEG 20 mM HEPES buffer. (B) The absorption and emission spectra of Qdot

    Journal: Macromolecular rapid communications

    Article Title: Ultrasensitive Detection of Proteins on Western Blots with Semiconducting Polymer Dots

    doi: 10.1002/marc.201200809

    Figure Lengend Snippet: (A) Structure of CN-PPV polymer and the absorption (dashed) and emission (solid) spectra of CN-PPV Pdot (black) and CN-PPV Pdot-streptavidin conjugate (CNPPV-Strep, red) in a 0.1% PEG 20 mM HEPES buffer. (B) The absorption and emission spectra of Qdot

    Article Snippet: To conjugate Pdots with streptavidin, to this 5 mL of Pdot solution, we added 100 μL of PEG (5 % w/v in water, MW 3350), 100 μL of 1 M HEPES buffer (pH 7.4), 300 μL of streptavidin (1 mg/mL in 20 mM HEPES buffer), and 50 μL of 1-ethyl-3-(3-dimethylaminopropyl) carbodiimide (EDC, from Sigma-Aldrich) (10 mg/mL in water).

    Techniques: