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  • 99
    Thermo Fisher n 2 hydroxyethylpiperazine n 2 ethanesulfonic acid hepes buffer solution
    In vitro activity of <t>mitoDPP-3.</t> a Structure of mitoDPP-3. b In vitro fluorescence assay of 1 µM mitoDPP-3 in <t>HEPES</t> (20 mM, pH 7.4, 150 mM NaCl, 0.1% Triton X-100) with either 50 nM purified APT1 or APT2 (λ ex 490/20 nm, λ em 545/20 nm). For plots, n = 3, error bars are ± s.e.m. c Fluorescence emission spectra at 30 min from probes as treated in ( b ). d UV–vis spectra of 25 µM mitoDPP-3 (black; normalized at 275 nm) and deprotected fluorophore product (gray; normalized at 513 nm) in HEPES (20 mM, pH 7.4, 150 mM NaCl, 0.1% Triton X-100). MitoDPP-3 shows UV–vis absorbance at 300 nm with extinction coefficient 12.6 × 10 3 M −1 cm −1
    N 2 Hydroxyethylpiperazine N 2 Ethanesulfonic Acid Hepes Buffer Solution, supplied by Thermo Fisher, used in various techniques. Bioz Stars score: 99/100, based on 5 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    Average 99 stars, based on 5 article reviews
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    99
    Millipore n 2 hydroxyethylpiperazine n 2 ethanesulfonic acid hepes buffer
    In vitro activity of <t>mitoDPP-3.</t> a Structure of mitoDPP-3. b In vitro fluorescence assay of 1 µM mitoDPP-3 in <t>HEPES</t> (20 mM, pH 7.4, 150 mM NaCl, 0.1% Triton X-100) with either 50 nM purified APT1 or APT2 (λ ex 490/20 nm, λ em 545/20 nm). For plots, n = 3, error bars are ± s.e.m. c Fluorescence emission spectra at 30 min from probes as treated in ( b ). d UV–vis spectra of 25 µM mitoDPP-3 (black; normalized at 275 nm) and deprotected fluorophore product (gray; normalized at 513 nm) in HEPES (20 mM, pH 7.4, 150 mM NaCl, 0.1% Triton X-100). MitoDPP-3 shows UV–vis absorbance at 300 nm with extinction coefficient 12.6 × 10 3 M −1 cm −1
    N 2 Hydroxyethylpiperazine N 2 Ethanesulfonic Acid Hepes Buffer, supplied by Millipore, used in various techniques. Bioz Stars score: 99/100, based on 8 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/n 2 hydroxyethylpiperazine n 2 ethanesulfonic acid hepes buffer/product/Millipore
    Average 99 stars, based on 8 article reviews
    Price from $9.99 to $1999.99
    n 2 hydroxyethylpiperazine n 2 ethanesulfonic acid hepes buffer - by Bioz Stars, 2020-08
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    85
    Atlanta Biologicals sterile hepes n 2 hydroxyethylpiperazine n 2 ethanesulfonic acid buffered rpmi 1640 culture medium
    In vitro activity of <t>mitoDPP-3.</t> a Structure of mitoDPP-3. b In vitro fluorescence assay of 1 µM mitoDPP-3 in <t>HEPES</t> (20 mM, pH 7.4, 150 mM NaCl, 0.1% Triton X-100) with either 50 nM purified APT1 or APT2 (λ ex 490/20 nm, λ em 545/20 nm). For plots, n = 3, error bars are ± s.e.m. c Fluorescence emission spectra at 30 min from probes as treated in ( b ). d UV–vis spectra of 25 µM mitoDPP-3 (black; normalized at 275 nm) and deprotected fluorophore product (gray; normalized at 513 nm) in HEPES (20 mM, pH 7.4, 150 mM NaCl, 0.1% Triton X-100). MitoDPP-3 shows UV–vis absorbance at 300 nm with extinction coefficient 12.6 × 10 3 M −1 cm −1
    Sterile Hepes N 2 Hydroxyethylpiperazine N 2 Ethanesulfonic Acid Buffered Rpmi 1640 Culture Medium, supplied by Atlanta Biologicals, used in various techniques. Bioz Stars score: 85/100, based on 4 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/sterile hepes n 2 hydroxyethylpiperazine n 2 ethanesulfonic acid buffered rpmi 1640 culture medium/product/Atlanta Biologicals
    Average 85 stars, based on 4 article reviews
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    96
    Tocris hepes buffer
    Glu-induced GABA release is not mediated by vesicular release. (A) Elevation of [GABA] o in acute rat hippocampal slices in control conditions (n = 6) and in the presence of glutamate receptor antagonists (n = 7; P = 0.014; 128.5±13.4% of pre-stimulus control). (B) Cytosolic GABA level as determined by [ 3 H]GABA uptake in the presence of 100 µM NNC-711 and Glu receptor antagonists in rat cerebrocortical <t>NPMV</t> fractions (n = 4; P = 0.82) (C) Elevation of [GABA] o in acute rat hippocampal slices in control conditions (n = 6) and in low-[Ca 2+ ]/high-[Mg 2+ ] buffer (n = 5; P = 0.91; 148.3±15.5% of pre-stimulus control). (D) Cytosolic GABA level as determined by [ 3 H]GABA uptake in the presence of 100 µM NNC-711 in low-[Ca 2+ ] buffer in rat cerebrocortical NPMV fractions (n = 3; P = 0.49). Low-[Ca 2+ ] buffer contained 145 mM NaCl, 5 mM KCl, 20 mM MgCl 2 , 10 mM glucose and 20 mM <t>HEPES</t> (pH 7.5).
    Hepes Buffer, supplied by Tocris, used in various techniques. Bioz Stars score: 96/100, based on 10 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/hepes buffer/product/Tocris
    Average 96 stars, based on 10 article reviews
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    98
    Millipore hepes buffered
    Kinetics of <t>methylprednisolone</t> diffusion in <t>HEPES</t> buffer (pH 7.4) under sink conditions (♦) and in vitro release profile of methylprednisolone from microparticles, in turn including (■) methylprednisolone 0.73%, ( ▲ ) methylprednisolone 1.64%, and (•) methylprednisolone 9.1% kept in release medium for ( A ) 10 hours, ( B ) 4 days and ( C ) 25 days. Data represent the mean ± standard deviation (n = 2).
    Hepes Buffered, supplied by Millipore, used in various techniques. Bioz Stars score: 98/100, based on 88 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/hepes buffered/product/Millipore
    Average 98 stars, based on 88 article reviews
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    hepes buffered - by Bioz Stars, 2020-08
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    99
    Millipore hepes buffer a
    (A) Structure of CN-PPV polymer and the absorption (dashed) and emission (solid) spectra of CN-PPV Pdot (black) and CN-PPV <t>Pdot-streptavidin</t> conjugate (CNPPV-Strep, red) in a 0.1% PEG 20 mM <t>HEPES</t> buffer. (B) The absorption and emission spectra of Qdot
    Hepes Buffer A, supplied by Millipore, used in various techniques. Bioz Stars score: 99/100, based on 919 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    hepes buffer a - by Bioz Stars, 2020-08
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    92
    Atlanta Biologicals hepes buffer
    (A) Structure of CN-PPV polymer and the absorption (dashed) and emission (solid) spectra of CN-PPV Pdot (black) and CN-PPV <t>Pdot-streptavidin</t> conjugate (CNPPV-Strep, red) in a 0.1% PEG 20 mM <t>HEPES</t> buffer. (B) The absorption and emission spectra of Qdot
    Hepes Buffer, supplied by Atlanta Biologicals, used in various techniques. Bioz Stars score: 92/100, based on 14 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/hepes buffer/product/Atlanta Biologicals
    Average 92 stars, based on 14 article reviews
    Price from $9.99 to $1999.99
    hepes buffer - by Bioz Stars, 2020-08
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    Image Search Results


    In vitro activity of mitoDPP-3. a Structure of mitoDPP-3. b In vitro fluorescence assay of 1 µM mitoDPP-3 in HEPES (20 mM, pH 7.4, 150 mM NaCl, 0.1% Triton X-100) with either 50 nM purified APT1 or APT2 (λ ex 490/20 nm, λ em 545/20 nm). For plots, n = 3, error bars are ± s.e.m. c Fluorescence emission spectra at 30 min from probes as treated in ( b ). d UV–vis spectra of 25 µM mitoDPP-3 (black; normalized at 275 nm) and deprotected fluorophore product (gray; normalized at 513 nm) in HEPES (20 mM, pH 7.4, 150 mM NaCl, 0.1% Triton X-100). MitoDPP-3 shows UV–vis absorbance at 300 nm with extinction coefficient 12.6 × 10 3 M −1 cm −1

    Journal: Nature Communications

    Article Title: Active and dynamic mitochondrial S-depalmitoylation revealed by targeted fluorescent probes

    doi: 10.1038/s41467-017-02655-1

    Figure Lengend Snippet: In vitro activity of mitoDPP-3. a Structure of mitoDPP-3. b In vitro fluorescence assay of 1 µM mitoDPP-3 in HEPES (20 mM, pH 7.4, 150 mM NaCl, 0.1% Triton X-100) with either 50 nM purified APT1 or APT2 (λ ex 490/20 nm, λ em 545/20 nm). For plots, n = 3, error bars are ± s.e.m. c Fluorescence emission spectra at 30 min from probes as treated in ( b ). d UV–vis spectra of 25 µM mitoDPP-3 (black; normalized at 275 nm) and deprotected fluorophore product (gray; normalized at 513 nm) in HEPES (20 mM, pH 7.4, 150 mM NaCl, 0.1% Triton X-100). MitoDPP-3 shows UV–vis absorbance at 300 nm with extinction coefficient 12.6 × 10 3 M −1 cm −1

    Article Snippet: In vitro kinetic assays of mitoDPP-2 and mitoDPP-3 In total 100 µL of 3 µM mitoDPP-2 or mitoDPP-3 in HEPES buffer (20 mM, pH = 7.4, 150 mM NaCl, 0.1% Triton X-100) were added to a 96-well optical bottom plate (Nunc 265301, Thermo Scientific) at room temperature.

    Techniques: In Vitro, Activity Assay, Fluorescence, Purification

    Synthesis and in vitro activity of mitoDPP-2. a Synthetic scheme for mitoDPP-2. b In vitro fluorescence assay of mitoDPP-2 (1 µM) in HEPES (20 mM, pH 7.4, 150 mM NaCl, 0.1% Triton X-100) with either 50 nM purified APT1 or APT2 (λ ex 490/20 nm, λ em 545/20 nm). Error bars are ± s.e.m. ( n = 3). c Fluorescence emission spectra at 30 min from probes as treated in ( b ). d UV–vis spectra of 25 µM mitoDPP-2 (black; normalized at 275 nm) and deprotected fluorophore product (gray; normalized at 513 nm) in HEPES (20 mM, pH 7.4, 150 mM NaCl, 0.1% Triton X-100). MitoDPP-2 shows UV–vis absorbance at 300 nm with extinction coefficient 8.7 × 10 3 M −1 cm −1 . The deprotected fluorophore product shows a major UV-Vis absorbance peak at 513 nm with extinction coefficient 11.8 × 10 3 M −1 cm −1

    Journal: Nature Communications

    Article Title: Active and dynamic mitochondrial S-depalmitoylation revealed by targeted fluorescent probes

    doi: 10.1038/s41467-017-02655-1

    Figure Lengend Snippet: Synthesis and in vitro activity of mitoDPP-2. a Synthetic scheme for mitoDPP-2. b In vitro fluorescence assay of mitoDPP-2 (1 µM) in HEPES (20 mM, pH 7.4, 150 mM NaCl, 0.1% Triton X-100) with either 50 nM purified APT1 or APT2 (λ ex 490/20 nm, λ em 545/20 nm). Error bars are ± s.e.m. ( n = 3). c Fluorescence emission spectra at 30 min from probes as treated in ( b ). d UV–vis spectra of 25 µM mitoDPP-2 (black; normalized at 275 nm) and deprotected fluorophore product (gray; normalized at 513 nm) in HEPES (20 mM, pH 7.4, 150 mM NaCl, 0.1% Triton X-100). MitoDPP-2 shows UV–vis absorbance at 300 nm with extinction coefficient 8.7 × 10 3 M −1 cm −1 . The deprotected fluorophore product shows a major UV-Vis absorbance peak at 513 nm with extinction coefficient 11.8 × 10 3 M −1 cm −1

    Article Snippet: In vitro kinetic assays of mitoDPP-2 and mitoDPP-3 In total 100 µL of 3 µM mitoDPP-2 or mitoDPP-3 in HEPES buffer (20 mM, pH = 7.4, 150 mM NaCl, 0.1% Triton X-100) were added to a 96-well optical bottom plate (Nunc 265301, Thermo Scientific) at room temperature.

    Techniques: In Vitro, Activity Assay, Fluorescence, Purification

    Glu-induced GABA release is not mediated by vesicular release. (A) Elevation of [GABA] o in acute rat hippocampal slices in control conditions (n = 6) and in the presence of glutamate receptor antagonists (n = 7; P = 0.014; 128.5±13.4% of pre-stimulus control). (B) Cytosolic GABA level as determined by [ 3 H]GABA uptake in the presence of 100 µM NNC-711 and Glu receptor antagonists in rat cerebrocortical NPMV fractions (n = 4; P = 0.82) (C) Elevation of [GABA] o in acute rat hippocampal slices in control conditions (n = 6) and in low-[Ca 2+ ]/high-[Mg 2+ ] buffer (n = 5; P = 0.91; 148.3±15.5% of pre-stimulus control). (D) Cytosolic GABA level as determined by [ 3 H]GABA uptake in the presence of 100 µM NNC-711 in low-[Ca 2+ ] buffer in rat cerebrocortical NPMV fractions (n = 3; P = 0.49). Low-[Ca 2+ ] buffer contained 145 mM NaCl, 5 mM KCl, 20 mM MgCl 2 , 10 mM glucose and 20 mM HEPES (pH 7.5).

    Journal: PLoS ONE

    Article Title: Glutamate Uptake Triggers Transporter-Mediated GABA Release from Astrocytes

    doi: 10.1371/journal.pone.0007153

    Figure Lengend Snippet: Glu-induced GABA release is not mediated by vesicular release. (A) Elevation of [GABA] o in acute rat hippocampal slices in control conditions (n = 6) and in the presence of glutamate receptor antagonists (n = 7; P = 0.014; 128.5±13.4% of pre-stimulus control). (B) Cytosolic GABA level as determined by [ 3 H]GABA uptake in the presence of 100 µM NNC-711 and Glu receptor antagonists in rat cerebrocortical NPMV fractions (n = 4; P = 0.82) (C) Elevation of [GABA] o in acute rat hippocampal slices in control conditions (n = 6) and in low-[Ca 2+ ]/high-[Mg 2+ ] buffer (n = 5; P = 0.91; 148.3±15.5% of pre-stimulus control). (D) Cytosolic GABA level as determined by [ 3 H]GABA uptake in the presence of 100 µM NNC-711 in low-[Ca 2+ ] buffer in rat cerebrocortical NPMV fractions (n = 3; P = 0.49). Low-[Ca 2+ ] buffer contained 145 mM NaCl, 5 mM KCl, 20 mM MgCl 2 , 10 mM glucose and 20 mM HEPES (pH 7.5).

    Article Snippet: Uptake studies were performed as follows: aliquots of NPMV fractions in HEPES buffer were preincubated with test compounds in the presence of NNC-711 (100 µM, Tocris) at 30°C for 10 min.

    Techniques:

    Kinetics of methylprednisolone diffusion in HEPES buffer (pH 7.4) under sink conditions (♦) and in vitro release profile of methylprednisolone from microparticles, in turn including (■) methylprednisolone 0.73%, ( ▲ ) methylprednisolone 1.64%, and (•) methylprednisolone 9.1% kept in release medium for ( A ) 10 hours, ( B ) 4 days and ( C ) 25 days. Data represent the mean ± standard deviation (n = 2).

    Journal: International Journal of Nanomedicine

    Article Title: Development of biodegradable methylprednisolone microparticles for treatment of articular pathology using a spray-drying technique

    doi: 10.2147/IJN.S39327

    Figure Lengend Snippet: Kinetics of methylprednisolone diffusion in HEPES buffer (pH 7.4) under sink conditions (♦) and in vitro release profile of methylprednisolone from microparticles, in turn including (■) methylprednisolone 0.73%, ( ▲ ) methylprednisolone 1.64%, and (•) methylprednisolone 9.1% kept in release medium for ( A ) 10 hours, ( B ) 4 days and ( C ) 25 days. Data represent the mean ± standard deviation (n = 2).

    Article Snippet: A weighed amount of microparticles equivalent to about 3 mg of methylprednisolone was resuspended in 10 mL of 10 mM HEPES buffered saline (150 mM NaCl, pH 7.4) by means of vortexing, then put into a dialysis tube (molecular weight cutoff 12,000 Da, Sigma Chemical Corporation, St Louis, MO, USA).

    Techniques: Diffusion-based Assay, In Vitro, Standard Deviation

    (A) Structure of CN-PPV polymer and the absorption (dashed) and emission (solid) spectra of CN-PPV Pdot (black) and CN-PPV Pdot-streptavidin conjugate (CNPPV-Strep, red) in a 0.1% PEG 20 mM HEPES buffer. (B) The absorption and emission spectra of Qdot

    Journal: Macromolecular rapid communications

    Article Title: Ultrasensitive Detection of Proteins on Western Blots with Semiconducting Polymer Dots

    doi: 10.1002/marc.201200809

    Figure Lengend Snippet: (A) Structure of CN-PPV polymer and the absorption (dashed) and emission (solid) spectra of CN-PPV Pdot (black) and CN-PPV Pdot-streptavidin conjugate (CNPPV-Strep, red) in a 0.1% PEG 20 mM HEPES buffer. (B) The absorption and emission spectra of Qdot

    Article Snippet: To conjugate Pdots with streptavidin, to this 5 mL of Pdot solution, we added 100 μL of PEG (5 % w/v in water, MW 3350), 100 μL of 1 M HEPES buffer (pH 7.4), 300 μL of streptavidin (1 mg/mL in 20 mM HEPES buffer), and 50 μL of 1-ethyl-3-(3-dimethylaminopropyl) carbodiimide (EDC, from Sigma-Aldrich) (10 mg/mL in water).

    Techniques: