Journal: Pharmaceutical Research
Article Title: Tuning Ciprofloxacin Release Profiles from Liposomally Encapsulated Nanocrystalline Drug
Figure Lengend Snippet: Evaluation of the effect of freeze-thaw in liquid nitrogen on the IVR profiles of CFI formulations at pH 6.0 containing 90 mg/ml sucrose and 0, 0.05 or 0.1% polysorbate 20 after freeze-thaw. The CFI formulations (at 12.5 mg/ml) were diluted to 50 μg/ml ciprofloxacin in HEPES buffered saline (HBS) prior to a 1:1 dilution in bovine serum to measure the release of ciprofloxacin after incubation at 37°C for up to 4 h. IVR profiles are shown for the CFI control prior to freeze-thaw ( blue diamonds ) or after freeze-thaw ( red circles ), CFI containing 0.05% polysorbate 20 after freeze-thaw ( green diamonds ), CFI containing 0.1% polysorbate 20 after freeze-thaw ( yellow triangles ). Duplicate samples were analyzed at each time point. Two simulated curves were generated by adjusting the release from the CFI sample without polysorbate 20 after freeze-thaw by assuming the addition of either 10% free drug ( green dashed line ) or 20% free drug ( yellow dashed line ) and normalizing the total drug release to 100%.
Article Snippet: The following materials were used for the preparation, characterization or analysis of the liposomal ciprofloxacin (CFI) formulations: sucrose (Sigma-Aldrich (St. Louis, MO)), HEPES, free acid (Avantor (Center Valley, PA)), sodium chloride (Amresco (Solon, OH)), HPLC grade methanol (Fisher Scientific (Fair Lawn, NJ)), triethylamine (TEA, JT Baker (USA)), polysorbate 20 (VWR Int. (West Chester, NJ)), polyethylene glycol dodecyl ether (Brij 30 (Sigma-Aldrich (Australia))), Donor Adult Bovine Serum (HyClone (Logan, Utah)), and Nanosep centrifugal filtration devices, 10 K and 30 K molecular weight (Pall Corporation (Ann Arbor, MI)).
Techniques: Incubation, Generated