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  • 99
    Thermo Fisher hepes
    Hepes, supplied by Thermo Fisher, used in various techniques. Bioz Stars score: 99/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/hepes/product/Thermo Fisher
    Average 99 stars, based on 1 article reviews
    Price from $9.99 to $1999.99
    hepes - by Bioz Stars, 2021-04
    99/100 stars
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    99
    Millipore hepes
    Hepes, supplied by Millipore, used in various techniques. Bioz Stars score: 99/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/hepes/product/Millipore
    Average 99 stars, based on 1 article reviews
    Price from $9.99 to $1999.99
    hepes - by Bioz Stars, 2021-04
    99/100 stars
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    hepes  (Roche)
    86
    Roche hepes
    ( Materials and methods ] to obtain K d values and are the average of three independent experiments. PCNA-AF488 or PCNA-MDCC and RFC were added sequentially to a solution of assay buffer and <t>ATP</t> (when present). Final reaction conditions: 30 mM <t>HEPES</t> pH 7.5, 150 mM NaCl, 2 mM DTT, 8 mM MgCl 2 , 0.5% glycerol, 750 mM maltose (when present), 0.5 mM ATP (when present), 10 nM PCNA-AF488 or 10 nM PCNA-MDCC, and 0–2.56 μM RFC.
    Hepes, supplied by Roche, used in various techniques. Bioz Stars score: 86/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/hepes/product/Roche
    Average 86 stars, based on 1 article reviews
    Price from $9.99 to $1999.99
    hepes - by Bioz Stars, 2021-04
    86/100 stars
      Buy from Supplier


    N/A
    HEPES hemisodium salt 4 2 Hydroxyethyl piperazine 1 ethanesulfonic acid hemisodium salt may be used in the preparation of HEPES buffer which has been described as one of the best
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    N/A
    Category Cell Products Cell Culture Products HEPES solution Size 100mL Price 30
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    N/A
    HEPES potassium salt 4 2 Hydroxyethyl piperazine 1 ethanesulfonic acid potassium salt may be used in the preparation of a HEPES KOH buffer used in protein extraction protocols and other
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    Image Search Results


    ( Materials and methods ] to obtain K d values and are the average of three independent experiments. PCNA-AF488 or PCNA-MDCC and RFC were added sequentially to a solution of assay buffer and ATP (when present). Final reaction conditions: 30 mM HEPES pH 7.5, 150 mM NaCl, 2 mM DTT, 8 mM MgCl 2 , 0.5% glycerol, 750 mM maltose (when present), 0.5 mM ATP (when present), 10 nM PCNA-AF488 or 10 nM PCNA-MDCC, and 0–2.56 μM RFC.

    Journal: Protein expression and purification

    Article Title: Improved solubility of replication factor C (RFC) Walker A mutants

    doi: 10.1016/j.pep.2012.03.010

    Figure Lengend Snippet: ( Materials and methods ] to obtain K d values and are the average of three independent experiments. PCNA-AF488 or PCNA-MDCC and RFC were added sequentially to a solution of assay buffer and ATP (when present). Final reaction conditions: 30 mM HEPES pH 7.5, 150 mM NaCl, 2 mM DTT, 8 mM MgCl 2 , 0.5% glycerol, 750 mM maltose (when present), 0.5 mM ATP (when present), 10 nM PCNA-AF488 or 10 nM PCNA-MDCC, and 0–2.56 μM RFC.

    Article Snippet: Concentrations of ATP (GE Healthcare) diluted with 30 mM HEPES pH 7.5 and ATPγS diluted with water (Roche Diagnostics) were determined by measuring the absorbance at 259 nm and using an extinction coefficient of 15,400 M−1 cm−1 .

    Techniques:

    ( Materials and methods ] to obtain K d values and are the average of three independent experiments for (B) and (C). Data in (D) and (E) are single experiments. DNA-RhX, RFC, and PCNA were added sequentially to a solution of assay buffer and ATPγS. Final reaction conditions: 30 mM HEPES pH 7.5, 150 mM NaCl, 2 mM DTT, 8 mM MgCl 2 , 0.5% glycerol, 0.5 mM ATPγS, 1 μM PCNA (without maltose) or 1.5 μM PCNA (with maltose), and 0–1 μM RFC (without maltose) or 0–1.5 μM RFC (with maltose).

    Journal: Protein expression and purification

    Article Title: Improved solubility of replication factor C (RFC) Walker A mutants

    doi: 10.1016/j.pep.2012.03.010

    Figure Lengend Snippet: ( Materials and methods ] to obtain K d values and are the average of three independent experiments for (B) and (C). Data in (D) and (E) are single experiments. DNA-RhX, RFC, and PCNA were added sequentially to a solution of assay buffer and ATPγS. Final reaction conditions: 30 mM HEPES pH 7.5, 150 mM NaCl, 2 mM DTT, 8 mM MgCl 2 , 0.5% glycerol, 0.5 mM ATPγS, 1 μM PCNA (without maltose) or 1.5 μM PCNA (with maltose), and 0–1 μM RFC (without maltose) or 0–1.5 μM RFC (with maltose).

    Article Snippet: Concentrations of ATP (GE Healthcare) diluted with 30 mM HEPES pH 7.5 and ATPγS diluted with water (Roche Diagnostics) were determined by measuring the absorbance at 259 nm and using an extinction coefficient of 15,400 M−1 cm−1 .

    Techniques: