Lonza
hepes Hepes, supplied by Lonza, used in various techniques. Bioz Stars score: 99/100, based on 2449 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more https://www.bioz.com/result/hepes/product/Lonza Average 99 stars, based on 2449 article reviews Price from $9.99 to $1999.99
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Thermo Fisher
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Millipore
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Thermo Fisher
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Mediatech
hepes Hepes, supplied by Mediatech, used in various techniques. Bioz Stars score: 92/100, based on 1465 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more https://www.bioz.com/result/hepes/product/Mediatech Average 92 stars, based on 1465 article reviews Price from $9.99 to $1999.99
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Corning Life Sciences
hepes Hepes, supplied by Corning Life Sciences, used in various techniques. Bioz Stars score: 92/100, based on 1282 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more https://www.bioz.com/result/hepes/product/Corning Life Sciences Average 92 stars, based on 1282 article reviews Price from $9.99 to $1999.99
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Fisher Scientific
hepes Hepes, supplied by Fisher Scientific, used in various techniques. Bioz Stars score: 92/100, based on 1083 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more https://www.bioz.com/result/hepes/product/Fisher Scientific Average 92 stars, based on 1083 article reviews Price from $9.99 to $1999.99
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Cellgro
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Thermo Fisher
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Millipore
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Thermo Fisher
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Biochrom
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Carl Roth GmbH
hepes ![]() Hepes, supplied by Carl Roth GmbH, used in various techniques. Bioz Stars score: 92/100, based on 640 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more https://www.bioz.com/result/hepes/product/Carl Roth GmbH Average 92 stars, based on 640 article reviews Price from $9.99 to $1999.99
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Tocris
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Mediatech
hepes buffer ![]() Hepes Buffer, supplied by Mediatech, used in various techniques. Bioz Stars score: 92/100, based on 574 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more https://www.bioz.com/result/hepes buffer/product/Mediatech Average 92 stars, based on 574 article reviews Price from $9.99 to $1999.99
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Bio-Rad
hepes ![]() Hepes, supplied by Bio-Rad, used in various techniques. Bioz Stars score: 92/100, based on 1096 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more https://www.bioz.com/result/hepes/product/Bio-Rad Average 92 stars, based on 1096 article reviews Price from $9.99 to $1999.99
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Millipore
hepes buffered saline ![]() Hepes Buffered Saline, supplied by Millipore, used in various techniques. Bioz Stars score: 99/100, based on 510 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more https://www.bioz.com/result/hepes buffered saline/product/Millipore Average 99 stars, based on 510 article reviews Price from $9.99 to $1999.99
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HEPES 4 2 Hydroxyethyl piperazine 1 ethane sulfonic acid has been described as one of the best all purpose buffers available for use in biological research HEPES is zwitterionic at
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This bottle contains filtered HEPES 1 M pH 7 5 The buffering range for HEPES is pH 6 8 8 2 and can be adjusted with KOH or NaOH It
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HEPES Sodium salt 4 2 Hydroxyethyl piperazine 1 ethanesulfonic acid sodium salt may be used in the preparation of HEPES buffer which has been described as one of the best
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Image Search Results

Journal: Frontiers in Immunology
Article Title: Serum and Serum Albumin Inhibit in vitro Formation of Neutrophil Extracellular Traps (NETs)
doi: 10.3389/fimmu.2019.00012
Figure Lengend Snippet: Extracellular NETotic DNA is rich in MPO. Representative fluorescence images of NETs induced by CaI (4 μM), PMA (100 nM), or LPS (25 μg/ml) activated in supplement-free RPMI/HEPES. The images show a clear colocalization of MPO (red) with the extracellular DNA-fibers (blue) of released NETs. Scale = 10 μm.
Article Snippet: Freshly isolated human or murine neutrophils were counted and suspended in RPMI 1640 (Lonza) containing 10 mM
Techniques: Fluorescence

Journal: Frontiers in Immunology
Article Title: Serum and Serum Albumin Inhibit in vitro Formation of Neutrophil Extracellular Traps (NETs)
doi: 10.3389/fimmu.2019.00012
Figure Lengend Snippet: Influence of serum and serum albumin supplements on NET formation of murine neutrophils. (A) Representative fluorescence images of nuclei of murine neutrophils (Hoechst) after stimulation with CaI (4 μM), PMA (100 nM) or LPS (25 μg/ml) for 180 min, respectively. NET formation of neutrophils was studied in RPMI/ HEPES without supplements and RPMI/ HEPES supplemented with 0.5% BSA or 2% hiFCS, as indicated. Chromatin decondensation is only inducible in RPMI/ HEPES (white arrow heads). Scale = 50 μm. (B) Percentage of decondensed nuclei/ NETs after stimulation with PMA (100 nM), CaI (4 μM), or LPS (10, 25, or 100 μg/ml) for 180 min. Murine neutrophils were studied in RPMI/ HEPES and RPMI/ HEPES supplemented with 0.5% BSA or 2% hiFCS. Addition of 0.5% BSA or 2% hiFCS to RPMI/ HEPES, inhibits chromatin decondensation induced by PMA, CaI and LPS completely. Error bars = mean ± SEM. ns = not significant. **** p
Article Snippet: Freshly isolated human or murine neutrophils were counted and suspended in RPMI 1640 (Lonza) containing 10 mM
Techniques: Fluorescence

Journal: Frontiers in Immunology
Article Title: Serum and Serum Albumin Inhibit in vitro Formation of Neutrophil Extracellular Traps (NETs)
doi: 10.3389/fimmu.2019.00012
Figure Lengend Snippet: Influence of serum and serum albumin supplements on NET formation of human neutrophils. (A) Representative fluorescence images of human neutrophils (chromatin stained by Hoechst) after stimulation with CaI (4 μM), PMA (100 nM), or LPS (25 μg/ml) for 180 min, respectively. NET formation of neutrophils was studied in RPMI 1,640 with 10 mM HEPES (RPMI/HEPES), RPMI/ HEPES + 0.5% human serum albumin (HSA) or + 1% heat inactivated (56°C) fetal calf serum (hiFCS). Chromatin decondensation induced by PMA is clearly visible with all three culture conditions, while LPS or CaI only cause NET formation in BSA- and HSA-free RPMI/HEPES. Scale = 50 μm. (B) Neutrophils were stimulated to undergo NET formation with CaI (4 μM), PMA (100 nM) or LPS (10, 25, or 100 μg/ml), respectively. Both, HSA and BSA inhibit CaI and LPS-induced formation of NETs (determined as percentage of decondensed nuclei/NETs of total neutrophils). NETosis stimulated by PMA is independent of serum albumin addition. Error bars = mean ± SEM. ns, not significant. * p
Article Snippet: Freshly isolated human or murine neutrophils were counted and suspended in RPMI 1640 (Lonza) containing 10 mM
Techniques: Fluorescence, Staining, Albumin Addition

Journal: Biochemistry
Article Title: The Interface between Escherichia coli Elongation Factor Tu and Aminoacyl-tRNA
doi: 10.1021/bi500533x
Figure Lengend Snippet: Comparison of yeast Phe-tRNA Phe binding to mutations made in E. coli EF-Tu (Table 1 ) with identical mutations made at orthologous sites in T. thermophilus EF-Tu. 49 E. coli numbering is used. Experiments performed in 50 mM HEPES pH 7, 30 mM KCl, 70 mM NH 4 Cl, 10 mM MgCl 2 , 10 μM GTP, 3 mM phosphoenolpyruvate, 50 μg/mL of pyruvate kinase, and 1 mM DTT at 0 °C for E. coli EF-Tu. ΔΔ G o values for each mutation were calculated relative to those of corresponding wild-type proteins (Δ G o = 10.1 ± 0.3 for E. coli and 9.8 ± 0.2 for T. thermophilus ). Since the complex was not stable enough, ΔΔ G o value displays the lower limit for T. thermophilus E259A, and the actual value is larger than 1.8. 49
Article Snippet: GTP Hydrolysis Rate Measurement Ternary complex was formed by mixing 0.4 μM EF-Tu with 1.2 μM Tyr-tRNATyr or 1.2 μM Tyr-T1 Tyr-tRNATyr , and 50 μM γ-32 P-GTP in 50 mM
Techniques: Binding Assay, Mutagenesis

Journal: Biochemistry
Article Title: The Interface between Escherichia coli Elongation Factor Tu and Aminoacyl-tRNA
doi: 10.1021/bi500533x
Figure Lengend Snippet: Time courses of ribosome-catalyzed GTP hydrolysis of ternary complexes containing Tyr-tRNA Tyr and the indicated EF-Tu mutations. Experiments were performed in 50 mM HEPES pH 7, 30 mM KCl, 70 mM NH 4 Cl, 10 mM MgCl 2 , 10 μM GTP, 3 mM phosphoenolpyruvate, 50 μg/mL of pyruvate kinase, and 1 mM DTT at 20 °C. Lines for each mutant indicate the best fit to a single binding equilibrium adjusted to an extent and a k obs. Values for all mutants are given in Table 2
Article Snippet: GTP Hydrolysis Rate Measurement Ternary complex was formed by mixing 0.4 μM EF-Tu with 1.2 μM Tyr-tRNATyr or 1.2 μM Tyr-T1 Tyr-tRNATyr , and 50 μM γ-32 P-GTP in 50 mM
Techniques: Mutagenesis, Binding Assay