hepatocyte growth factor hgf Search Results


  • Logo
  • About
  • News
  • Press Release
  • Team
  • Advisors
  • Partners
  • Contact
  • Bioz Stars
  • Bioz vStars
  • 99
    Thermo Fisher recombinant human hepatocyte growth factor
    Recombinant Human Hepatocyte Growth Factor, supplied by Thermo Fisher, used in various techniques. Bioz Stars score: 99/100, based on 33 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/recombinant human hepatocyte growth factor/product/Thermo Fisher
    Average 99 stars, based on 33 article reviews
    Price from $9.99 to $1999.99
    recombinant human hepatocyte growth factor - by Bioz Stars, 2020-09
    99/100 stars
      Buy from Supplier

    99
    Millipore hepatocyte growth factor
    Cell migration assay and response to <t>HGF</t> and <t>GDNF.</t> A , Confocal image of RMS explants from Plxnb2 +/− and Plxnb2 −/− P4–P5 mice stained with Topro-3 after 36 h in culture in the absence of any growth factor or in the presence of 50 ng/ml HGF or 200 ng/ml GDNF. B , C , Quantification of the number of cells migrating out of explants cultured with HGF ( B ) or GDNF ( C ). No significant difference was observed between wild-type, Plxnb2 +/− , and Plxnb2 −/− explants cultured with or without growth factors. HGF promoted migration, but GDNF had no effect. Error bars indicate SEM. Scale bar, 200 μm.
    Hepatocyte Growth Factor, supplied by Millipore, used in various techniques. Bioz Stars score: 99/100, based on 445 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/hepatocyte growth factor/product/Millipore
    Average 99 stars, based on 445 article reviews
    Price from $9.99 to $1999.99
    hepatocyte growth factor - by Bioz Stars, 2020-09
    99/100 stars
      Buy from Supplier

    88
    Morishita Jintan r hepatocyte growth factor
    Cell migration assay and response to <t>HGF</t> and <t>GDNF.</t> A , Confocal image of RMS explants from Plxnb2 +/− and Plxnb2 −/− P4–P5 mice stained with Topro-3 after 36 h in culture in the absence of any growth factor or in the presence of 50 ng/ml HGF or 200 ng/ml GDNF. B , C , Quantification of the number of cells migrating out of explants cultured with HGF ( B ) or GDNF ( C ). No significant difference was observed between wild-type, Plxnb2 +/− , and Plxnb2 −/− explants cultured with or without growth factors. HGF promoted migration, but GDNF had no effect. Error bars indicate SEM. Scale bar, 200 μm.
    R Hepatocyte Growth Factor, supplied by Morishita Jintan, used in various techniques. Bioz Stars score: 88/100, based on 30 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/r hepatocyte growth factor/product/Morishita Jintan
    Average 88 stars, based on 30 article reviews
    Price from $9.99 to $1999.99
    r hepatocyte growth factor - by Bioz Stars, 2020-09
    88/100 stars
      Buy from Supplier

    93
    Thermo Fisher hepatocyte growth factor
    Cell migration assay and response to <t>HGF</t> and <t>GDNF.</t> A , Confocal image of RMS explants from Plxnb2 +/− and Plxnb2 −/− P4–P5 mice stained with Topro-3 after 36 h in culture in the absence of any growth factor or in the presence of 50 ng/ml HGF or 200 ng/ml GDNF. B , C , Quantification of the number of cells migrating out of explants cultured with HGF ( B ) or GDNF ( C ). No significant difference was observed between wild-type, Plxnb2 +/− , and Plxnb2 −/− explants cultured with or without growth factors. HGF promoted migration, but GDNF had no effect. Error bars indicate SEM. Scale bar, 200 μm.
    Hepatocyte Growth Factor, supplied by Thermo Fisher, used in various techniques. Bioz Stars score: 93/100, based on 105 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/hepatocyte growth factor/product/Thermo Fisher
    Average 93 stars, based on 105 article reviews
    Price from $9.99 to $1999.99
    hepatocyte growth factor - by Bioz Stars, 2020-09
    93/100 stars
      Buy from Supplier

    97
    R&D Systems recombinant human hepatocyte growth factor
    Survival of cell sheets transplanted onto an immunodeficient rat kidney for 28 days. (A) SV40-T–immunostained image of <t>hepatocyte</t> <t>growth</t> <t>factor</t> transgenic (HGF-tg) mesothelial cell (MC) sheets (left panel) and MC sheets (right panel) on day 7 after transplantation onto an immunodeficient rat kidney. Higher-magnification photomicrographs of the border (red dashed line) between the capsule and cortex are shown on the right side of each panel, and they correspond to the boxed areas. Scale bars, 200 μ m in the left side of each panel; 20 μ m in the right side of each panel. (B) SV40-T–immunostained image of HGF-tg MC sheets on day 28 after transplantation onto an immunodeficient rat kidney. Lower (right panel) and higher (left panel) magnification images. Scale bars, 200 μ m in the left panel; 20 μ m in the right panel. (C) Fractions of simian virus 40 (SV40)–positive cells on the renal surface on day 7 after transplanting MC sheets or HGF-tg MC sheets. (D) <t>Human</t> hepatocyte growth factor (hHGF)–immunostained image of an HGF-tg MC sheet on day 28 after transplantation onto an immunodeficient rat kidney. Black arrows indicate hHGF-positive cells in the renal capsule. Scale bar, 50 μ m. (E) Phospho-cMET–immunostained images of unilateral ureteral obstruction (UUO)–treated kidneys on days 7 (upper panels) and 28 (lower panels) after UUO in the no transplantation (no sheet), MC sheet, or HGF-tg MC sheet groups. P
    Recombinant Human Hepatocyte Growth Factor, supplied by R&D Systems, used in various techniques. Bioz Stars score: 97/100, based on 85 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/recombinant human hepatocyte growth factor/product/R&D Systems
    Average 97 stars, based on 85 article reviews
    Price from $9.99 to $1999.99
    recombinant human hepatocyte growth factor - by Bioz Stars, 2020-09
    97/100 stars
      Buy from Supplier

    93
    Thermo Fisher hepatocyte growth factor hgf
    Survival of cell sheets transplanted onto an immunodeficient rat kidney for 28 days. (A) SV40-T–immunostained image of <t>hepatocyte</t> <t>growth</t> <t>factor</t> transgenic (HGF-tg) mesothelial cell (MC) sheets (left panel) and MC sheets (right panel) on day 7 after transplantation onto an immunodeficient rat kidney. Higher-magnification photomicrographs of the border (red dashed line) between the capsule and cortex are shown on the right side of each panel, and they correspond to the boxed areas. Scale bars, 200 μ m in the left side of each panel; 20 μ m in the right side of each panel. (B) SV40-T–immunostained image of HGF-tg MC sheets on day 28 after transplantation onto an immunodeficient rat kidney. Lower (right panel) and higher (left panel) magnification images. Scale bars, 200 μ m in the left panel; 20 μ m in the right panel. (C) Fractions of simian virus 40 (SV40)–positive cells on the renal surface on day 7 after transplanting MC sheets or HGF-tg MC sheets. (D) <t>Human</t> hepatocyte growth factor (hHGF)–immunostained image of an HGF-tg MC sheet on day 28 after transplantation onto an immunodeficient rat kidney. Black arrows indicate hHGF-positive cells in the renal capsule. Scale bar, 50 μ m. (E) Phospho-cMET–immunostained images of unilateral ureteral obstruction (UUO)–treated kidneys on days 7 (upper panels) and 28 (lower panels) after UUO in the no transplantation (no sheet), MC sheet, or HGF-tg MC sheet groups. P
    Hepatocyte Growth Factor Hgf, supplied by Thermo Fisher, used in various techniques. Bioz Stars score: 93/100, based on 182 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/hepatocyte growth factor hgf/product/Thermo Fisher
    Average 93 stars, based on 182 article reviews
    Price from $9.99 to $1999.99
    hepatocyte growth factor hgf - by Bioz Stars, 2020-09
    93/100 stars
      Buy from Supplier

    95
    Millipore recombinant human hepatocyte growth factor
    Gelsolin mediates <t>HGF-induced</t> E-cadherin downregulation via <t>PI3K-Akt</t> pathway A. Western blot of MKN28 cells transfected with ctsi or siGelsolin before treatment with HGF for 0-120 min and analyzed for gelsolin, phosphorylated Akt and Akt protein levels. B. Western blot of MKN28 cells pre-incubated with 10, 20 and 40μM LY294002 for 1h before treatment with HGF for 48h and analyzed for gelsolin, phosphorylated Akt and Akt protein levels. C. Western blot of MKN28 cells treated as in A. and analyzed for protein levels of EMT markers E-Cadherin, Zeb2, Twist and Snail. D. Proximity ligation assay of MKN28 cells treated with 10ng/ml HGF for 1h and stained with PI3K and Gelsolin antibodies. Top: Microscopy images of cells. Red spots are representative of the interactions between PI3K and Gelsolin, each red spot is equivalent to one molecular interaction. Nuclei are stained with DAPI. Images were acquired using a fluorescence microscope at x400 magnification. Scale Bar = 20μm. Bottom: Quantitative analysis of number of molecular interactions counted from 3 representative images taken at random fields from 2 experiments. Values represent mean ± SD, n = 2, * P
    Recombinant Human Hepatocyte Growth Factor, supplied by Millipore, used in various techniques. Bioz Stars score: 95/100, based on 85 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/recombinant human hepatocyte growth factor/product/Millipore
    Average 95 stars, based on 85 article reviews
    Price from $9.99 to $1999.99
    recombinant human hepatocyte growth factor - by Bioz Stars, 2020-09
    95/100 stars
      Buy from Supplier

    92
    Bio-Rad hepatocyte growth factor hgf
    Inflammatory cytokines and growth factors are impacted by H 2 S therapy following intestinal I/R. Intestinal levels of IL-6, IL-9, IL-10, <t>VEGF,</t> and fibroblast growth factor 2 (FGF-2) are decreased with H 2 S therapy following intestinal ischemia. H 2 S decreases in IL-9, IL-10, and VEGF appear to be facilitated by intact eNOS, whereas decreases in IL-6 and FGF-2 may work through H 2 S-independent pathways. Hepatocyte growth factor <t>(HGF)</t> does not appear to be affected by H 2 S therapy or ablation of eNOS (* P
    Hepatocyte Growth Factor Hgf, supplied by Bio-Rad, used in various techniques. Bioz Stars score: 92/100, based on 37 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/hepatocyte growth factor hgf/product/Bio-Rad
    Average 92 stars, based on 37 article reviews
    Price from $9.99 to $1999.99
    hepatocyte growth factor hgf - by Bioz Stars, 2020-09
    92/100 stars
      Buy from Supplier

    99
    PeproTech hepatocyte growth factor hgf
    Migration assays. The chemotaxis of bone marrow derived mesenchymal stem cells (BMSCs; open) and ear mesenchymal stem cell (EMSCs; checkered) in response to increasing concentrations of hepatocyte growth factor <t>(HGF)</t> and stromal-derived <t>factor-1α</t>
    Hepatocyte Growth Factor Hgf, supplied by PeproTech, used in various techniques. Bioz Stars score: 99/100, based on 186 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/hepatocyte growth factor hgf/product/PeproTech
    Average 99 stars, based on 186 article reviews
    Price from $9.99 to $1999.99
    hepatocyte growth factor hgf - by Bioz Stars, 2020-09
    99/100 stars
      Buy from Supplier

    93
    Abcam hepatocyte growth factor hgf
    FGF-2 and <t>HGF</t> gene expression in fibroblasts in <t>ASC-CM.</t> (A) FGF-2, (B) HGF were measured by real-time PCR. When comparing groups treated with and without TGF-β1, TGF-β1 appears to be a crucial regulator FGF-2 gene expression. There was a close relationship between concentrations of ASC-CM and HGF gene expression. 100% ASC-CM increases HGF gene expression relative to 0 and 10% ASC-CM. Solid bars represent analysis of variance with Tukey’s posttest. * P value ≤ .05; † P value ≤ .01; ‡ P value ≤ .001; n = 4 (A) and n = 5 (B). ASC-CM , adipose-derived stem cells–conditioned medium; FGF-2 , basic fibroblast growth factor; HFF-1 , human foreskin fibroblast; HGF , hepatocyte growth factor; PCR, polymerase chain reaction; TGF-β1 , transforming growth factor-β1.
    Hepatocyte Growth Factor Hgf, supplied by Abcam, used in various techniques. Bioz Stars score: 93/100, based on 102 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/hepatocyte growth factor hgf/product/Abcam
    Average 93 stars, based on 102 article reviews
    Price from $9.99 to $1999.99
    hepatocyte growth factor hgf - by Bioz Stars, 2020-09
    93/100 stars
      Buy from Supplier

    99
    PeproTech hepatocyte growth factor
    FGF-2 and <t>HGF</t> gene expression in fibroblasts in <t>ASC-CM.</t> (A) FGF-2, (B) HGF were measured by real-time PCR. When comparing groups treated with and without TGF-β1, TGF-β1 appears to be a crucial regulator FGF-2 gene expression. There was a close relationship between concentrations of ASC-CM and HGF gene expression. 100% ASC-CM increases HGF gene expression relative to 0 and 10% ASC-CM. Solid bars represent analysis of variance with Tukey’s posttest. * P value ≤ .05; † P value ≤ .01; ‡ P value ≤ .001; n = 4 (A) and n = 5 (B). ASC-CM , adipose-derived stem cells–conditioned medium; FGF-2 , basic fibroblast growth factor; HFF-1 , human foreskin fibroblast; HGF , hepatocyte growth factor; PCR, polymerase chain reaction; TGF-β1 , transforming growth factor-β1.
    Hepatocyte Growth Factor, supplied by PeproTech, used in various techniques. Bioz Stars score: 99/100, based on 501 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/hepatocyte growth factor/product/PeproTech
    Average 99 stars, based on 501 article reviews
    Price from $9.99 to $1999.99
    hepatocyte growth factor - by Bioz Stars, 2020-09
    99/100 stars
      Buy from Supplier

    92
    Abcam hepatocyte growth factor
    FGF-2 and <t>HGF</t> gene expression in fibroblasts in <t>ASC-CM.</t> (A) FGF-2, (B) HGF were measured by real-time PCR. When comparing groups treated with and without TGF-β1, TGF-β1 appears to be a crucial regulator FGF-2 gene expression. There was a close relationship between concentrations of ASC-CM and HGF gene expression. 100% ASC-CM increases HGF gene expression relative to 0 and 10% ASC-CM. Solid bars represent analysis of variance with Tukey’s posttest. * P value ≤ .05; † P value ≤ .01; ‡ P value ≤ .001; n = 4 (A) and n = 5 (B). ASC-CM , adipose-derived stem cells–conditioned medium; FGF-2 , basic fibroblast growth factor; HFF-1 , human foreskin fibroblast; HGF , hepatocyte growth factor; PCR, polymerase chain reaction; TGF-β1 , transforming growth factor-β1.
    Hepatocyte Growth Factor, supplied by Abcam, used in various techniques. Bioz Stars score: 92/100, based on 24 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/hepatocyte growth factor/product/Abcam
    Average 92 stars, based on 24 article reviews
    Price from $9.99 to $1999.99
    hepatocyte growth factor - by Bioz Stars, 2020-09
    92/100 stars
      Buy from Supplier

    93
    RayBiotech hepatocyte growth factor hgf
    The concentrations of IL‐6, <t>MCP‐1,</t> <t>HGF,</t> GRO, IL‐8, and OPG in culture supernatants (A) and cell lysates (B) determined by enzyme‐linked immunosorbent assay. Data represent the means ± SDs ( n = 4). ∗, p
    Hepatocyte Growth Factor Hgf, supplied by RayBiotech, used in various techniques. Bioz Stars score: 93/100, based on 27 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/hepatocyte growth factor hgf/product/RayBiotech
    Average 93 stars, based on 27 article reviews
    Price from $9.99 to $1999.99
    hepatocyte growth factor hgf - by Bioz Stars, 2020-09
    93/100 stars
      Buy from Supplier

    90
    Santa Cruz Biotechnology hepatocyte growth factor hgf
    Photomicrographs of mouse liver, <t>immunohistochemical</t> staining for GST-1, PARP, MMP-9, and <t>HGF-1.</t> a GST-1 expression was increased dose-dependently by DCE-2.5 to DCE-10. b PARP was markedly positive in the DCE-2.5 group. c MMP-9 was strongly positive in the DCE-2.5 group. d HGF-1 was gradually and dose-dependently increased by DCE
    Hepatocyte Growth Factor Hgf, supplied by Santa Cruz Biotechnology, used in various techniques. Bioz Stars score: 90/100, based on 25 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/hepatocyte growth factor hgf/product/Santa Cruz Biotechnology
    Average 90 stars, based on 25 article reviews
    Price from $9.99 to $1999.99
    hepatocyte growth factor hgf - by Bioz Stars, 2020-09
    90/100 stars
      Buy from Supplier

    94
    Sino Biological anti hgf antibody
    Photomicrographs of mouse liver, <t>immunohistochemical</t> staining for GST-1, PARP, MMP-9, and <t>HGF-1.</t> a GST-1 expression was increased dose-dependently by DCE-2.5 to DCE-10. b PARP was markedly positive in the DCE-2.5 group. c MMP-9 was strongly positive in the DCE-2.5 group. d HGF-1 was gradually and dose-dependently increased by DCE
    Anti Hgf Antibody, supplied by Sino Biological, used in various techniques. Bioz Stars score: 94/100, based on 13 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/anti hgf antibody/product/Sino Biological
    Average 94 stars, based on 13 article reviews
    Price from $9.99 to $1999.99
    anti hgf antibody - by Bioz Stars, 2020-09
    94/100 stars
      Buy from Supplier

    92
    Prospec hepatocyte growth factor
    Photomicrographs of mouse liver, <t>immunohistochemical</t> staining for GST-1, PARP, MMP-9, and <t>HGF-1.</t> a GST-1 expression was increased dose-dependently by DCE-2.5 to DCE-10. b PARP was markedly positive in the DCE-2.5 group. c MMP-9 was strongly positive in the DCE-2.5 group. d HGF-1 was gradually and dose-dependently increased by DCE
    Hepatocyte Growth Factor, supplied by Prospec, used in various techniques. Bioz Stars score: 92/100, based on 18 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/hepatocyte growth factor/product/Prospec
    Average 92 stars, based on 18 article reviews
    Price from $9.99 to $1999.99
    hepatocyte growth factor - by Bioz Stars, 2020-09
    92/100 stars
      Buy from Supplier

    99
    Abcam anti hgf antibody
    Photomicrographs of mouse liver, <t>immunohistochemical</t> staining for GST-1, PARP, MMP-9, and <t>HGF-1.</t> a GST-1 expression was increased dose-dependently by DCE-2.5 to DCE-10. b PARP was markedly positive in the DCE-2.5 group. c MMP-9 was strongly positive in the DCE-2.5 group. d HGF-1 was gradually and dose-dependently increased by DCE
    Anti Hgf Antibody, supplied by Abcam, used in various techniques. Bioz Stars score: 99/100, based on 133 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/anti hgf antibody/product/Abcam
    Average 99 stars, based on 133 article reviews
    Price from $9.99 to $1999.99
    anti hgf antibody - by Bioz Stars, 2020-09
    99/100 stars
      Buy from Supplier

    Image Search Results


    Cell migration assay and response to HGF and GDNF. A , Confocal image of RMS explants from Plxnb2 +/− and Plxnb2 −/− P4–P5 mice stained with Topro-3 after 36 h in culture in the absence of any growth factor or in the presence of 50 ng/ml HGF or 200 ng/ml GDNF. B , C , Quantification of the number of cells migrating out of explants cultured with HGF ( B ) or GDNF ( C ). No significant difference was observed between wild-type, Plxnb2 +/− , and Plxnb2 −/− explants cultured with or without growth factors. HGF promoted migration, but GDNF had no effect. Error bars indicate SEM. Scale bar, 200 μm.

    Journal: The Journal of Neuroscience

    Article Title: Plexin-B2 Regulates the Proliferation and Migration of Neuroblasts in the Postnatal and Adult Subventricular Zone

    doi: 10.1523/JNEUROSCI.0344-12.2012

    Figure Lengend Snippet: Cell migration assay and response to HGF and GDNF. A , Confocal image of RMS explants from Plxnb2 +/− and Plxnb2 −/− P4–P5 mice stained with Topro-3 after 36 h in culture in the absence of any growth factor or in the presence of 50 ng/ml HGF or 200 ng/ml GDNF. B , C , Quantification of the number of cells migrating out of explants cultured with HGF ( B ) or GDNF ( C ). No significant difference was observed between wild-type, Plxnb2 +/− , and Plxnb2 −/− explants cultured with or without growth factors. HGF promoted migration, but GDNF had no effect. Error bars indicate SEM. Scale bar, 200 μm.

    Article Snippet: To analyze the effect of growth factors, either glial cell line-derived neurotrophic factor (GDNF) (200 ng/ml; R & D Systems) or hepatocyte growth factor (HGF) (50 ng/ml; Sigma-Aldrich) were added in the culture media.

    Techniques: Cell Migration Assay, Mouse Assay, Staining, Cell Culture, Migration

    Development of VIM promoter–driven RFP expression system using CRC cell lines. ( a ) Structure for VIM promoter–driven RFP expression vector without or with VIM 3′-UTR, VR or VRV3 vector, respectively. Photographs of HCT116 cells stably transfected with VR and VRV3 vectors, HCT116-VR and HCT116-VRV3. Scale bars: 50 μm. MCS, multi-cloning site. ( b ) Photographs of HCT116-VRV3 and RKO-VRV3 cells treated without or with TNF-α (20 ng/ml), IL-1β (1 ng/ml), TGF-β (10 ng/ml), HGF (50 ng/ml), IGF-1 (20 ng/ml), EGF (20 ng/ml), or bFGF (10 ng/ml) for 48 h. Scale bars: 50 μm. ( c ) expression of RFP in HCT116-VRV3 and RKO-VRV3 cells treated without or with TNF-α (20 ng/ml), IL-1β (1 ng/ml), TGF-β (10 ng/ml), HGF (50 ng/ml), IGF-1 (20 ng/ml), EGF (20 ng/ml), or bFGF (10 ng/ml) for 48 h. β-actin was used as a loading control.

    Journal: Scientific Reports

    Article Title: Visualization of epithelial-mesenchymal transition in an inflammatory microenvironment–colorectal cancer network

    doi: 10.1038/s41598-019-52816-z

    Figure Lengend Snippet: Development of VIM promoter–driven RFP expression system using CRC cell lines. ( a ) Structure for VIM promoter–driven RFP expression vector without or with VIM 3′-UTR, VR or VRV3 vector, respectively. Photographs of HCT116 cells stably transfected with VR and VRV3 vectors, HCT116-VR and HCT116-VRV3. Scale bars: 50 μm. MCS, multi-cloning site. ( b ) Photographs of HCT116-VRV3 and RKO-VRV3 cells treated without or with TNF-α (20 ng/ml), IL-1β (1 ng/ml), TGF-β (10 ng/ml), HGF (50 ng/ml), IGF-1 (20 ng/ml), EGF (20 ng/ml), or bFGF (10 ng/ml) for 48 h. Scale bars: 50 μm. ( c ) expression of RFP in HCT116-VRV3 and RKO-VRV3 cells treated without or with TNF-α (20 ng/ml), IL-1β (1 ng/ml), TGF-β (10 ng/ml), HGF (50 ng/ml), IGF-1 (20 ng/ml), EGF (20 ng/ml), or bFGF (10 ng/ml) for 48 h. β-actin was used as a loading control.

    Article Snippet: Induction of RFP expression in CRC cells in an inflammatory microenvironment Recombinant human TNF-α, IL-1β, TGF-β, HGF, insulin-like growth factor 1 (IGF-1), EGF, and bFGF were obtained from Sigma-Aldrich (St. Louis, MO, USA).

    Techniques: Expressing, Plasmid Preparation, Stable Transfection, Transfection, Clone Assay

    Selective Induction of CRTC3 in response to ERK1/2 activation. a) Western blot showing phosphorylation of CRTC3 at Ser 391 upon stimulation of ERK1/2 in B16F1 cells (SCF – stem cell factor (30ng/ml); HGF – hepatocyte growth factor (20ng/ml), TPA (200nM) b) Rescue of melanogenesis in CRTC3 KO B16F1 cells after transfection with indicated constructs. Cells were treated with 5μM FSK for 60h, 48h after transfection c) Melanin production related to cAMP content in B16F1 cells stimulated with indicated compounds for 60h. d) Western blot of a representative sub-cellular fractionation experiment after treatment with 800nM FSK or 30ng/ml SCF for 20min. e) f) Western blots of indicated human cell lines in the basal state and treatment with 200nM ERK1/2 inhibitor SCH772984 for 8h. g) Immunofluorescence of HEK293T and A375 cells stained with hCRTC3(414-432) antibody and DAPI. Bar is 20μM h) Luciferase reporter assay in A375 cells transfected with MITF proximal promoter construct. Treatments and mutations of regulatory elements are indicated. Cells were pretreated with 200nM ERK1/2 inhibitor SCH772984 for 6h before adding 100μM IBMX for 5h.

    Journal: bioRxiv

    Article Title: CRTC3 is a coincidence detector for cAMP and growth factor signals in melanocytes and melanoma

    doi: 10.1101/2020.07.14.202697

    Figure Lengend Snippet: Selective Induction of CRTC3 in response to ERK1/2 activation. a) Western blot showing phosphorylation of CRTC3 at Ser 391 upon stimulation of ERK1/2 in B16F1 cells (SCF – stem cell factor (30ng/ml); HGF – hepatocyte growth factor (20ng/ml), TPA (200nM) b) Rescue of melanogenesis in CRTC3 KO B16F1 cells after transfection with indicated constructs. Cells were treated with 5μM FSK for 60h, 48h after transfection c) Melanin production related to cAMP content in B16F1 cells stimulated with indicated compounds for 60h. d) Western blot of a representative sub-cellular fractionation experiment after treatment with 800nM FSK or 30ng/ml SCF for 20min. e) f) Western blots of indicated human cell lines in the basal state and treatment with 200nM ERK1/2 inhibitor SCH772984 for 8h. g) Immunofluorescence of HEK293T and A375 cells stained with hCRTC3(414-432) antibody and DAPI. Bar is 20μM h) Luciferase reporter assay in A375 cells transfected with MITF proximal promoter construct. Treatments and mutations of regulatory elements are indicated. Cells were pretreated with 200nM ERK1/2 inhibitor SCH772984 for 6h before adding 100μM IBMX for 5h.

    Article Snippet: Compounds used for cell treatments in these experiments were SCF (S9915, Sigma), HGF (H9661, Sigma), FSK (F6886; Sigma) and IBMX (I5879, Sigma).

    Techniques: Activation Assay, Western Blot, Transfection, Construct, Cell Fractionation, Immunofluorescence, Staining, Luciferase, Reporter Assay

    Survival of cell sheets transplanted onto an immunodeficient rat kidney for 28 days. (A) SV40-T–immunostained image of hepatocyte growth factor transgenic (HGF-tg) mesothelial cell (MC) sheets (left panel) and MC sheets (right panel) on day 7 after transplantation onto an immunodeficient rat kidney. Higher-magnification photomicrographs of the border (red dashed line) between the capsule and cortex are shown on the right side of each panel, and they correspond to the boxed areas. Scale bars, 200 μ m in the left side of each panel; 20 μ m in the right side of each panel. (B) SV40-T–immunostained image of HGF-tg MC sheets on day 28 after transplantation onto an immunodeficient rat kidney. Lower (right panel) and higher (left panel) magnification images. Scale bars, 200 μ m in the left panel; 20 μ m in the right panel. (C) Fractions of simian virus 40 (SV40)–positive cells on the renal surface on day 7 after transplanting MC sheets or HGF-tg MC sheets. (D) Human hepatocyte growth factor (hHGF)–immunostained image of an HGF-tg MC sheet on day 28 after transplantation onto an immunodeficient rat kidney. Black arrows indicate hHGF-positive cells in the renal capsule. Scale bar, 50 μ m. (E) Phospho-cMET–immunostained images of unilateral ureteral obstruction (UUO)–treated kidneys on days 7 (upper panels) and 28 (lower panels) after UUO in the no transplantation (no sheet), MC sheet, or HGF-tg MC sheet groups. P

    Journal: Journal of the American Society of Nephrology : JASN

    Article Title: Hepatocyte Growth Factor–Secreting Mesothelial Cell Sheets Suppress Progressive Fibrosis in a Rat Model of CKD

    doi: 10.1681/ASN.2018050556

    Figure Lengend Snippet: Survival of cell sheets transplanted onto an immunodeficient rat kidney for 28 days. (A) SV40-T–immunostained image of hepatocyte growth factor transgenic (HGF-tg) mesothelial cell (MC) sheets (left panel) and MC sheets (right panel) on day 7 after transplantation onto an immunodeficient rat kidney. Higher-magnification photomicrographs of the border (red dashed line) between the capsule and cortex are shown on the right side of each panel, and they correspond to the boxed areas. Scale bars, 200 μ m in the left side of each panel; 20 μ m in the right side of each panel. (B) SV40-T–immunostained image of HGF-tg MC sheets on day 28 after transplantation onto an immunodeficient rat kidney. Lower (right panel) and higher (left panel) magnification images. Scale bars, 200 μ m in the left panel; 20 μ m in the right panel. (C) Fractions of simian virus 40 (SV40)–positive cells on the renal surface on day 7 after transplanting MC sheets or HGF-tg MC sheets. (D) Human hepatocyte growth factor (hHGF)–immunostained image of an HGF-tg MC sheet on day 28 after transplantation onto an immunodeficient rat kidney. Black arrows indicate hHGF-positive cells in the renal capsule. Scale bar, 50 μ m. (E) Phospho-cMET–immunostained images of unilateral ureteral obstruction (UUO)–treated kidneys on days 7 (upper panels) and 28 (lower panels) after UUO in the no transplantation (no sheet), MC sheet, or HGF-tg MC sheet groups. P

    Article Snippet: After UUO, rats in the HGF injection group received 200 pg recombinant human hepatocyte growth factor (hHGF; R & D Systems, Minneapolis, MN) via the tail vein every 24 hours for 7 days.

    Techniques: Transgenic Assay, Transplantation Assay

    Hepatocyte growth factor transgenic (HGF-tg) mesothelial cell (MC) sheets for transplantation onto kidneys secreted HGF. (A) Macroscopic image of an HGF-tg MC sheet detached from a temperature-responsive culture dish. Scale bar, 10 mm. (B) Levels of human hepatocyte growth factor (hHGF) measured by ELISA in the supernatant of MC and HGF-tg MC sheets. (C) Hematoxylin and eosin staining of an HGF-tg MC sheet section. Scale bar, 100 μ m. (D) Schematic representation of the experimental procedure used to transplant HGF-tg MC sheets in a rat obstructive nephropathy model. The left kidney was exposed from the back of the immunodeficient rat, and the ureter was ligated. The renal capsule on the dorsal side was removed from the surface of the rat kidney with tweezers. In the HGF-tg MC sheet group, two HGF-tg MC sheets were placed on the site where the renal capsule was removed. In the MC sheet group, two MC sheets (lacking the HGF gene) were inserted. * P

    Journal: Journal of the American Society of Nephrology : JASN

    Article Title: Hepatocyte Growth Factor–Secreting Mesothelial Cell Sheets Suppress Progressive Fibrosis in a Rat Model of CKD

    doi: 10.1681/ASN.2018050556

    Figure Lengend Snippet: Hepatocyte growth factor transgenic (HGF-tg) mesothelial cell (MC) sheets for transplantation onto kidneys secreted HGF. (A) Macroscopic image of an HGF-tg MC sheet detached from a temperature-responsive culture dish. Scale bar, 10 mm. (B) Levels of human hepatocyte growth factor (hHGF) measured by ELISA in the supernatant of MC and HGF-tg MC sheets. (C) Hematoxylin and eosin staining of an HGF-tg MC sheet section. Scale bar, 100 μ m. (D) Schematic representation of the experimental procedure used to transplant HGF-tg MC sheets in a rat obstructive nephropathy model. The left kidney was exposed from the back of the immunodeficient rat, and the ureter was ligated. The renal capsule on the dorsal side was removed from the surface of the rat kidney with tweezers. In the HGF-tg MC sheet group, two HGF-tg MC sheets were placed on the site where the renal capsule was removed. In the MC sheet group, two MC sheets (lacking the HGF gene) were inserted. * P

    Article Snippet: After UUO, rats in the HGF injection group received 200 pg recombinant human hepatocyte growth factor (hHGF; R & D Systems, Minneapolis, MN) via the tail vein every 24 hours for 7 days.

    Techniques: Transgenic Assay, Transplantation Assay, Enzyme-linked Immunosorbent Assay, Staining

    BMP4 inhibits proliferation in vitro . A : CyQUANT cell proliferation assay using primary hepatocyte cultures. Hepatocyte proliferation is inhibited by BMP4 and partially rescued by EGF, but not hepatocyte growth factor (HGF) ( n = 5–25). BMP4 (20

    Journal: American Journal of Physiology - Gastrointestinal and Liver Physiology

    Article Title: BMP4 is a novel paracrine inhibitor of liver regeneration

    doi: 10.1152/ajpgi.00105.2012

    Figure Lengend Snippet: BMP4 inhibits proliferation in vitro . A : CyQUANT cell proliferation assay using primary hepatocyte cultures. Hepatocyte proliferation is inhibited by BMP4 and partially rescued by EGF, but not hepatocyte growth factor (HGF) ( n = 5–25). BMP4 (20

    Article Snippet: For the proliferation assay, cells were grown for 4 days in serum-free medium with one change and with growth factors as indicated [recombinant human hepatocyte growth factor (HGF), recombinant human EGF, and/or recombinant human BMP4 (R & D Systems)].

    Techniques: In Vitro, CyQUANT Assay, Proliferation Assay

    Gelsolin mediates HGF-induced E-cadherin downregulation via PI3K-Akt pathway A. Western blot of MKN28 cells transfected with ctsi or siGelsolin before treatment with HGF for 0-120 min and analyzed for gelsolin, phosphorylated Akt and Akt protein levels. B. Western blot of MKN28 cells pre-incubated with 10, 20 and 40μM LY294002 for 1h before treatment with HGF for 48h and analyzed for gelsolin, phosphorylated Akt and Akt protein levels. C. Western blot of MKN28 cells treated as in A. and analyzed for protein levels of EMT markers E-Cadherin, Zeb2, Twist and Snail. D. Proximity ligation assay of MKN28 cells treated with 10ng/ml HGF for 1h and stained with PI3K and Gelsolin antibodies. Top: Microscopy images of cells. Red spots are representative of the interactions between PI3K and Gelsolin, each red spot is equivalent to one molecular interaction. Nuclei are stained with DAPI. Images were acquired using a fluorescence microscope at x400 magnification. Scale Bar = 20μm. Bottom: Quantitative analysis of number of molecular interactions counted from 3 representative images taken at random fields from 2 experiments. Values represent mean ± SD, n = 2, * P

    Journal: Oncotarget

    Article Title: Gelsolin-mediated activation of PI3K/Akt pathway is crucial for hepatocyte growth factor-induced cell scattering in gastric carcinoma

    doi: 10.18632/oncotarget.8603

    Figure Lengend Snippet: Gelsolin mediates HGF-induced E-cadherin downregulation via PI3K-Akt pathway A. Western blot of MKN28 cells transfected with ctsi or siGelsolin before treatment with HGF for 0-120 min and analyzed for gelsolin, phosphorylated Akt and Akt protein levels. B. Western blot of MKN28 cells pre-incubated with 10, 20 and 40μM LY294002 for 1h before treatment with HGF for 48h and analyzed for gelsolin, phosphorylated Akt and Akt protein levels. C. Western blot of MKN28 cells treated as in A. and analyzed for protein levels of EMT markers E-Cadherin, Zeb2, Twist and Snail. D. Proximity ligation assay of MKN28 cells treated with 10ng/ml HGF for 1h and stained with PI3K and Gelsolin antibodies. Top: Microscopy images of cells. Red spots are representative of the interactions between PI3K and Gelsolin, each red spot is equivalent to one molecular interaction. Nuclei are stained with DAPI. Images were acquired using a fluorescence microscope at x400 magnification. Scale Bar = 20μm. Bottom: Quantitative analysis of number of molecular interactions counted from 3 representative images taken at random fields from 2 experiments. Values represent mean ± SD, n = 2, * P

    Article Snippet: Human recombinant hepatocyte growth factor (HGF) was purchased from Sigma while PI3K inhibitor LY294002 and was purchased from Promega, Madison, WI, USA.

    Techniques: Western Blot, Transfection, Incubation, Proximity Ligation Assay, Staining, Microscopy, Fluorescence

    Inflammatory cytokines and growth factors are impacted by H 2 S therapy following intestinal I/R. Intestinal levels of IL-6, IL-9, IL-10, VEGF, and fibroblast growth factor 2 (FGF-2) are decreased with H 2 S therapy following intestinal ischemia. H 2 S decreases in IL-9, IL-10, and VEGF appear to be facilitated by intact eNOS, whereas decreases in IL-6 and FGF-2 may work through H 2 S-independent pathways. Hepatocyte growth factor (HGF) does not appear to be affected by H 2 S therapy or ablation of eNOS (* P

    Journal: American Journal of Physiology - Gastrointestinal and Liver Physiology

    Article Title: Hydrogen sulfide improves intestinal recovery following ischemia by endothelial nitric oxide-dependent mechanisms

    doi: 10.1152/ajpgi.00444.2016

    Figure Lengend Snippet: Inflammatory cytokines and growth factors are impacted by H 2 S therapy following intestinal I/R. Intestinal levels of IL-6, IL-9, IL-10, VEGF, and fibroblast growth factor 2 (FGF-2) are decreased with H 2 S therapy following intestinal ischemia. H 2 S decreases in IL-9, IL-10, and VEGF appear to be facilitated by intact eNOS, whereas decreases in IL-6 and FGF-2 may work through H 2 S-independent pathways. Hepatocyte growth factor (HGF) does not appear to be affected by H 2 S therapy or ablation of eNOS (* P

    Article Snippet: Murine intestinal levels of interleukin 6 (IL-6), IL-9, IL-10, C-X-C ligand 10 (IP10), macrophage inflammatory protein 2 (MIP-2), granulocyte-colony stimulating factor (G-CSF), MIP-1α, eotaxin, vascular endothelial growth factor (VEGF), fibroblast growth factor 2 (FGF-2), hepatocyte growth factor (HGF), and C-X-C ligand 1 (KC) were quantified using a Bio-Plex 200 multiplex beaded assay system (Bio-Rad, Hercules, CA) with customizable multiplex plates for murine inflammatory cytokines (Millipore, Billerica, MA).

    Techniques:

    Migration assays. The chemotaxis of bone marrow derived mesenchymal stem cells (BMSCs; open) and ear mesenchymal stem cell (EMSCs; checkered) in response to increasing concentrations of hepatocyte growth factor (HGF) and stromal-derived factor-1α

    Journal: Stem Cells and Development

    Article Title: Cell Growth Characteristics, Differentiation Frequency, and Immunophenotype of Adult Ear Mesenchymal Stem Cells

    doi: 10.1089/scd.2009.0051

    Figure Lengend Snippet: Migration assays. The chemotaxis of bone marrow derived mesenchymal stem cells (BMSCs; open) and ear mesenchymal stem cell (EMSCs; checkered) in response to increasing concentrations of hepatocyte growth factor (HGF) and stromal-derived factor-1α

    Article Snippet: The 700 μL of the final dilution of the recombinant Stromal-Derived Factor-1α (SDF-1α) or Hepatocyte Growth Factor (HGF) (both obtained from Peprotech, Rocky Hill, NJ) were used as a chemoattractant in the lower chamber at concentrations of 1, 25, and 500 ng/mL.

    Techniques: Migration, Chemotaxis Assay, Derivative Assay

    FGF-2 and HGF gene expression in fibroblasts in ASC-CM. (A) FGF-2, (B) HGF were measured by real-time PCR. When comparing groups treated with and without TGF-β1, TGF-β1 appears to be a crucial regulator FGF-2 gene expression. There was a close relationship between concentrations of ASC-CM and HGF gene expression. 100% ASC-CM increases HGF gene expression relative to 0 and 10% ASC-CM. Solid bars represent analysis of variance with Tukey’s posttest. * P value ≤ .05; † P value ≤ .01; ‡ P value ≤ .001; n = 4 (A) and n = 5 (B). ASC-CM , adipose-derived stem cells–conditioned medium; FGF-2 , basic fibroblast growth factor; HFF-1 , human foreskin fibroblast; HGF , hepatocyte growth factor; PCR, polymerase chain reaction; TGF-β1 , transforming growth factor-β1.

    Journal: Journal of Burn Care & Research: Official Publication of the American Burn Association

    Article Title: The Effects of Adipose Stem Cell–Conditioned Media on Fibrogenesis of Dermal Fibroblasts Stimulated by Transforming Growth Factor-β1

    doi: 10.1097/BCR.0000000000000558

    Figure Lengend Snippet: FGF-2 and HGF gene expression in fibroblasts in ASC-CM. (A) FGF-2, (B) HGF were measured by real-time PCR. When comparing groups treated with and without TGF-β1, TGF-β1 appears to be a crucial regulator FGF-2 gene expression. There was a close relationship between concentrations of ASC-CM and HGF gene expression. 100% ASC-CM increases HGF gene expression relative to 0 and 10% ASC-CM. Solid bars represent analysis of variance with Tukey’s posttest. * P value ≤ .05; † P value ≤ .01; ‡ P value ≤ .001; n = 4 (A) and n = 5 (B). ASC-CM , adipose-derived stem cells–conditioned medium; FGF-2 , basic fibroblast growth factor; HFF-1 , human foreskin fibroblast; HGF , hepatocyte growth factor; PCR, polymerase chain reaction; TGF-β1 , transforming growth factor-β1.

    Article Snippet: Analysis of Cytokines in ASC-CM by Enzyme-Linked Immunosorbent Assay Concentrations of hepatocyte growth factor (HGF) and basic fibroblast growth factor (FGF-2) cytokines in ASC-CM were measured by enzyme-linked immunosorbent assay (ELISA, Abcam) according to the manufacturer’s instructions.

    Techniques: Expressing, Real-time Polymerase Chain Reaction, Derivative Assay, Polymerase Chain Reaction

    The concentrations of IL‐6, MCP‐1, HGF, GRO, IL‐8, and OPG in culture supernatants (A) and cell lysates (B) determined by enzyme‐linked immunosorbent assay. Data represent the means ± SDs ( n = 4). ∗, p

    Journal: Stem Cells Translational Medicine

    Article Title: Human Menstrual Blood‐Derived Stem Cells Ameliorate Liver Fibrosis in Mice by Targeting Hepatic Stellate Cells via Paracrine Mediators

    doi: 10.5966/sctm.2015-0265

    Figure Lengend Snippet: The concentrations of IL‐6, MCP‐1, HGF, GRO, IL‐8, and OPG in culture supernatants (A) and cell lysates (B) determined by enzyme‐linked immunosorbent assay. Data represent the means ± SDs ( n = 4). ∗, p

    Article Snippet: Based on the antibody array results, we examined protein levels of monocyte chemoattractant protein‐1 (MCP‐1), interleukin‐6 (IL‐6), hepatocyte growth factor (HGF), growth‐related oncogene (GRO), IL‐8, and osteoprotegerin (OPG) using enzyme‐linked immunosorbent assay (ELISA) kits (RayBiotech) both in culture supernatants and cell lysates, according to a previously described protocol .

    Techniques: Enzyme-linked Immunosorbent Assay

    Cytokine secretion in MenSC/LX‐2 cocultures. (A): Representative array images are shown ( n = 4). Cytokines of interest (MCP‐1, IL‐6, HGF, GRO, IL‐8, and OPG) are highlighted with red boxes and arrows, accordingly. (B): Differential cytokine expression shown as a heat map. Red, green, and black colors represent upregulated, downregulated, and unchanged expression, respectively. (C, D): Results from GO (C) and pathway (D) bioinformatic analyses. The top 10 pathways are shown with their associated p values. Abbreviations: GO, Gene Ontology; GRO, growth‐related oncogene; HGF, hepatocyte growth factor; IL‐6, interleukin 6; MCP‐1, monocyte chemoattractant protein‐1; MenSC, menstrual blood‐derived stem cell; OPG, osteoprotegerin.

    Journal: Stem Cells Translational Medicine

    Article Title: Human Menstrual Blood‐Derived Stem Cells Ameliorate Liver Fibrosis in Mice by Targeting Hepatic Stellate Cells via Paracrine Mediators

    doi: 10.5966/sctm.2015-0265

    Figure Lengend Snippet: Cytokine secretion in MenSC/LX‐2 cocultures. (A): Representative array images are shown ( n = 4). Cytokines of interest (MCP‐1, IL‐6, HGF, GRO, IL‐8, and OPG) are highlighted with red boxes and arrows, accordingly. (B): Differential cytokine expression shown as a heat map. Red, green, and black colors represent upregulated, downregulated, and unchanged expression, respectively. (C, D): Results from GO (C) and pathway (D) bioinformatic analyses. The top 10 pathways are shown with their associated p values. Abbreviations: GO, Gene Ontology; GRO, growth‐related oncogene; HGF, hepatocyte growth factor; IL‐6, interleukin 6; MCP‐1, monocyte chemoattractant protein‐1; MenSC, menstrual blood‐derived stem cell; OPG, osteoprotegerin.

    Article Snippet: Based on the antibody array results, we examined protein levels of monocyte chemoattractant protein‐1 (MCP‐1), interleukin‐6 (IL‐6), hepatocyte growth factor (HGF), growth‐related oncogene (GRO), IL‐8, and osteoprotegerin (OPG) using enzyme‐linked immunosorbent assay (ELISA) kits (RayBiotech) both in culture supernatants and cell lysates, according to a previously described protocol .

    Techniques: Expressing, Derivative Assay

    Photomicrographs of mouse liver, immunohistochemical staining for GST-1, PARP, MMP-9, and HGF-1. a GST-1 expression was increased dose-dependently by DCE-2.5 to DCE-10. b PARP was markedly positive in the DCE-2.5 group. c MMP-9 was strongly positive in the DCE-2.5 group. d HGF-1 was gradually and dose-dependently increased by DCE

    Journal: Maxillofacial Plastic and Reconstructive Surgery

    Article Title: In vivo protein expression changes in mouse livers treated with dialyzed coffee extract as determined by IP-HPLC

    doi: 10.1186/s40902-018-0183-z

    Figure Lengend Snippet: Photomicrographs of mouse liver, immunohistochemical staining for GST-1, PARP, MMP-9, and HGF-1. a GST-1 expression was increased dose-dependently by DCE-2.5 to DCE-10. b PARP was markedly positive in the DCE-2.5 group. c MMP-9 was strongly positive in the DCE-2.5 group. d HGF-1 was gradually and dose-dependently increased by DCE

    Article Snippet: Histological and immunohistochemical observation Mouse liver microsections in 4 μm thickness were subjected to hematoxylin and eosin staining, and serial microsections were also prepared for immunohistochemical staining using representative antisera of hepatocyte growth factor (HGF), glutathione S-transferase-1 (GST-1), poly-ADP ribose polymerase (PARP), or tumor necrosis factor-α (TNFα) (Santa Cruz Biotech.

    Techniques: Immunohistochemistry, Staining, Expressing