Journal: The Journal of Physiology
Article Title: RAAS inhibitors directly reduce diabetes‐induced renal fibrosis via growth factor inhibition
Figure Lengend Snippet: Profibrotic factor production in human kidney 2 (HK‐2) cells on normal, high glucose (HG), mannitol and renin‐angiotensin‐aldosterone system inhibitor (RAASi) treatment A , transforming growth factor β ( TGFB1 ), platelet‐derived growth factor B ( PDGFB ), and connective tissue growth factor ( CTGF ) mRNA expression in HK‐2 proximal tubular epithelial cells on HG or osmotic control mannitol treatment. B, TGFB1 , PDGFB , and CTGF mRNA expression in HK‐2 cells on HG and RAASi treatment. Values are presented as means ± 95% confidence intervals; n = 6 wells/group; one‐way ANOVA followed by Bonferroniʹs multiple‐comparison post hoc test; * P
Article Snippet: Cell cultures and treatments Human kidney 2 proximal tubular epithelial (HK‐2) cells (LGC Standards, ATCC Cat. No. CRL‐2190, RRID:CVCL_0302) were cultured in DMEM containing 5.5 mM glucose (Gibco, supplied by Life Technologies, Carlsbad, CA, USA) and normal rat kidney fibroblast (NRK‐49F) cells (LGC Standards, ATCC Cat. No. CRL‐1570, RRID:CVCL_2144) were maintained in DMEM containing 25 mM glucose (Gibco), both supplemented with 10% FBS (Gibco), 1% penicillin/streptomycin and 1% L‐glutamine, and incubated at 5% CO2 and 37°C.
Techniques: Derivative Assay, Expressing