Journal: The Journal of Biological Chemistry

Article Title: Diabetes and Pancreatic Exocrine Dysfunction Due to Mutations in the Carboxyl Ester Lipase Gene-Maturity Onset Diabetes of the Young (CEL-MODY): A PROTEIN MISFOLDING DISEASE*

doi: 10.1074/jbc.M111.222679

Figure Lengend Snippet: Prolonged time course for the secretion of CEL. CEL-MUT protein appears to be more stable than the CEL-WT. HEK293 cells were metabolically labeled and chased for the indicated time periods, and the proteins in cell extract and growth medium were immunoprecipitated

Article Snippet: Similarly, the highly positively charged TR domain of CEL-MUT would favor its interaction with membranes (phospholipid headgroups) as actually observed as aggregates at the plasma membrane of HEK293 cells in this study ( A ).

Techniques: Metabolic Labelling, Labeling, Immunoprecipitation

Journal: The Journal of Biological Chemistry

Article Title: Diabetes and Pancreatic Exocrine Dysfunction Due to Mutations in the Carboxyl Ester Lipase Gene-Maturity Onset Diabetes of the Young (CEL-MODY): A PROTEIN MISFOLDING DISEASE*

doi: 10.1074/jbc.M111.222679

Figure Lengend Snippet: UPR in HEK293 cells expressing different CEL proteins. Detergent-soluble proteins in lysates of cells expressing CEL-WT, CEL-MUT, and EV were analyzed by immunoblotting using specific antibodies (see under “Experimental Procedures”). A

Article Snippet: Similarly, the highly positively charged TR domain of CEL-MUT would favor its interaction with membranes (phospholipid headgroups) as actually observed as aggregates at the plasma membrane of HEK293 cells in this study ( A ).

Techniques: Expressing

Journal: The Journal of Biological Chemistry

Article Title: Diabetes and Pancreatic Exocrine Dysfunction Due to Mutations in the Carboxyl Ester Lipase Gene-Maturity Onset Diabetes of the Young (CEL-MODY): A PROTEIN MISFOLDING DISEASE*

doi: 10.1074/jbc.M111.222679

Figure Lengend Snippet: CEL-MUT protein forms higher oligomeric forms that are SDS-resistant and Triton X-100-insoluble. A , metabolically labeling of HEK293 cells, stably expressing CEL-WT ( left panel ) and CEL-MUT ( right panel ). Subsequently, the cells were lysed, and the CEL

Article Snippet: Similarly, the highly positively charged TR domain of CEL-MUT would favor its interaction with membranes (phospholipid headgroups) as actually observed as aggregates at the plasma membrane of HEK293 cells in this study ( A ).

Techniques: Metabolic Labelling, Labeling, Stable Transfection, Expressing

Journal: The Journal of Biological Chemistry

Article Title: Diabetes and Pancreatic Exocrine Dysfunction Due to Mutations in the Carboxyl Ester Lipase Gene-Maturity Onset Diabetes of the Young (CEL-MODY): A PROTEIN MISFOLDING DISEASE*

doi: 10.1074/jbc.M111.222679

Figure Lengend Snippet: CEL-MUT forms extra- and intracellular aggregates in HEK293 cells. HEK293 cells stably transfected with CEL-MUT and CEL-WT were processed for immunoperoxidase electron microscopy as described under “Experimental Procedures.” A , extracellular

Article Snippet: Similarly, the highly positively charged TR domain of CEL-MUT would favor its interaction with membranes (phospholipid headgroups) as actually observed as aggregates at the plasma membrane of HEK293 cells in this study ( A ).

Techniques: Stable Transfection, Transfection, Electron Microscopy

Journal: The Journal of Biological Chemistry

Article Title: Diabetes and Pancreatic Exocrine Dysfunction Due to Mutations in the Carboxyl Ester Lipase Gene-Maturity Onset Diabetes of the Young (CEL-MODY): A PROTEIN MISFOLDING DISEASE*

doi: 10.1074/jbc.M111.222679

Figure Lengend Snippet: In vitro N - and O -linked deglycosylation of CEL proteins variants. Acetone-precipitated proteins from the cell-free growth medium of HEK293 cells, stably expressing CEL-WT and CEL-MUT, were subjected to N -linked ( N -glycosidase F) and/or O -linked ( O -glycosidase

Article Snippet: Similarly, the highly positively charged TR domain of CEL-MUT would favor its interaction with membranes (phospholipid headgroups) as actually observed as aggregates at the plasma membrane of HEK293 cells in this study ( A ).

Techniques: In Vitro, Stable Transfection, Expressing

Journal: The Journal of Biological Chemistry

Article Title: Diabetes and Pancreatic Exocrine Dysfunction Due to Mutations in the Carboxyl Ester Lipase Gene-Maturity Onset Diabetes of the Young (CEL-MODY): A PROTEIN MISFOLDING DISEASE*

doi: 10.1074/jbc.M111.222679

Figure Lengend Snippet: Time course for the secretion of CEL-WT and CEL-MUT by stably transfected HEK293 cells. A , pulse-chase analysis of HEK293 stably expressing CEL-WT ( left panel ) and CEL-MUT ( right panel ). The cells were metabolically labeled with [ 35 S]Met/Cys for 5 min,

Article Snippet: Similarly, the highly positively charged TR domain of CEL-MUT would favor its interaction with membranes (phospholipid headgroups) as actually observed as aggregates at the plasma membrane of HEK293 cells in this study ( A ).

Techniques: Stable Transfection, Transfection, Pulse Chase, Expressing, Metabolic Labelling, Labeling

Journal: Molecular and Cellular Biology

Article Title: Differential Roles of Hypoxia-Inducible Factor 1? (HIF-1?) and HIF-2? in Hypoxic Gene Regulation

doi: 10.1128/MCB.23.24.9361-9374.2003

Figure Lengend Snippet: Establishment of HEK293 TET-on HIF-1αDPA and HIF-2αDPA clones. (A) HIF-α mRNA expression is tightly regulated by doxycycline. Northern blot analysis of HIF-1α and HIF-2α mRNA expression in HEK293 TET-on HIF-1αDPA clone 130 (left) and HEK293 TET-on HIF-2αDPA clone 63 (right) treated with different amounts of doxycycline for 20 h. (B) Doxycycline (1 μg/ml for 20 h) induced HIF-1α mRNA and protein expression in two independent HEK293 TET-on HIF-1αDPA clones (left) and HIF-2α mRNA and protein expression in two independent HEK293 TET-on HIF-2αDPA clones (right). Dox. conc., concentration of doxycycline.

Article Snippet: HIF-1αDPA and HIF-2αDPA cDNAs under transcriptional control of the TRE were stably transfected into HEK293 TET-on cells, which express the reverse TET-controlled transactivator.

Techniques: Expressing, Northern Blot, Concentration Assay