heat-shock transformation Search Results


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  • 99
    Qiagen plasmid maxi kit
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    New England Biolabs heat shock
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    Thermo Fisher heat shock transformation
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    Thermo Fisher heat shock method
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    Thermo Fisher dh5 α heat shock competent cells
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    Thermo Fisher heat shock competent top10 bacteria
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    Thermo Fisher heat shock competent top10 escherichia coli strain
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    Thermo Fisher heat shock competent e coli
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    Agilent technologies heat shock competent e coli bl21 gold
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    Millipore heat shock protein 72 73 hsp70
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    Thermo Fisher mouse anti hsp60
    Expression time course of scFv which is soluble expressed in E. coli BL21/ (DE3). Crude extracts (30 μg protein per lane) from indicated time points before/after induction with 1 mM IPTG were separated by SDS-PAGE and blotted on PVDF membranes. Western Blot was performed as described in Methods . The upper lane shows target protein expression, the lower lane ( E. coli 60 kDa <t>HSP60</t> protein) represents the loading control. Mock represents the negative control (lysates of bacteria transformed with empty vector). Experiment shown is representative for at least three repeated experiments
    Mouse Anti Hsp60, supplied by Thermo Fisher, used in various techniques. Bioz Stars score: 99/100, based on 23 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    Thermo Fisher gene exp hspb7 hs00205296 m1
    Steroid receptor associated and regulated protein and <t>HSPB7</t> copy number changes across different malignancies. Heat map demonstrates mean gene‐level estimates for SRARP and HSPB7 in 35 TCGA datasets and TCGA Pan‐Cancer dataset. The GISTIC2_thersholded method was utilized to measure SRARP and HSPB7 gene‐level copy number changes. Next, significance levels for copy number changes between cancer types and their matched normal tissues were calculated using the Kruskal–Wallis test. For each TCGA dataset, the name of dataset, SRARP and HSPB7 copy number changes (copy no.), and sample size are shown. * P
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    TaKaRa groes groel protein chaperone expressing plasmid
    Steroid receptor associated and regulated protein and <t>HSPB7</t> copy number changes across different malignancies. Heat map demonstrates mean gene‐level estimates for SRARP and HSPB7 in 35 TCGA datasets and TCGA Pan‐Cancer dataset. The GISTIC2_thersholded method was utilized to measure SRARP and HSPB7 gene‐level copy number changes. Next, significance levels for copy number changes between cancer types and their matched normal tissues were calculated using the Kruskal–Wallis test. For each TCGA dataset, the name of dataset, SRARP and HSPB7 copy number changes (copy no.), and sample size are shown. * P
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    ATGen recombinant hsp60 protein
    Systemic levels of <t>Hsp60</t> were similar in healthy individuals and patients with different forms of periodontitis. The concentration of Hsp60 in the serum was measured by two-site ELISA. The distribution of transformed Hsp60 concentrations in the serum of healthy subjects (H, n = 28) and patients diagnosed with chronic (ChP, n = 29) or aggressive (AgP, n = 30) periodontitis is visualised as box-and-whisker plots (SPSS)
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    Thermo Fisher streptomycin
    Systemic levels of <t>Hsp60</t> were similar in healthy individuals and patients with different forms of periodontitis. The concentration of Hsp60 in the serum was measured by two-site ELISA. The distribution of transformed Hsp60 concentrations in the serum of healthy subjects (H, n = 28) and patients diagnosed with chronic (ChP, n = 29) or aggressive (AgP, n = 30) periodontitis is visualised as box-and-whisker plots (SPSS)
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    Millipore all trans retinal
    Systemic levels of <t>Hsp60</t> were similar in healthy individuals and patients with different forms of periodontitis. The concentration of Hsp60 in the serum was measured by two-site ELISA. The distribution of transformed Hsp60 concentrations in the serum of healthy subjects (H, n = 28) and patients diagnosed with chronic (ChP, n = 29) or aggressive (AgP, n = 30) periodontitis is visualised as box-and-whisker plots (SPSS)
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    Image Search Results


    Expression time course of scFv which is soluble expressed in E. coli BL21/ (DE3). Crude extracts (30 μg protein per lane) from indicated time points before/after induction with 1 mM IPTG were separated by SDS-PAGE and blotted on PVDF membranes. Western Blot was performed as described in Methods . The upper lane shows target protein expression, the lower lane ( E. coli 60 kDa HSP60 protein) represents the loading control. Mock represents the negative control (lysates of bacteria transformed with empty vector). Experiment shown is representative for at least three repeated experiments

    Journal: BMC Biotechnology

    Article Title: Novel avian single-chain fragment variable (scFv) targets dietary gluten and related natural grain prolamins, toxic entities of celiac disease

    doi: 10.1186/s12896-015-0223-z

    Figure Lengend Snippet: Expression time course of scFv which is soluble expressed in E. coli BL21/ (DE3). Crude extracts (30 μg protein per lane) from indicated time points before/after induction with 1 mM IPTG were separated by SDS-PAGE and blotted on PVDF membranes. Western Blot was performed as described in Methods . The upper lane shows target protein expression, the lower lane ( E. coli 60 kDa HSP60 protein) represents the loading control. Mock represents the negative control (lysates of bacteria transformed with empty vector). Experiment shown is representative for at least three repeated experiments

    Article Snippet: Unlike otherwise noted, all following incubation steps were performed at room temperature with agitation at 350 rpm: Membranes were blocked with 3 % (w/v) skim milk powder (Fluka) in TBS for 1 h. Membranes were incubated simultaneously with mouse-anti-penta-His antibody (Qiagen) at a 1:500 dilution and mouse-anti-HSP60 (Heat Shock Protein 60, Mouse monoclonal antibody, Thermo Scientific) at a 1:7500 dilution in TBST supplemented with 1 % milk powder for 1 h. Membranes were washed twice for 10 min with TBST, followed by incubation in anti-mouse-IgG-HRP (Amersham ECL Western Blotting Reagent Pack, GE healthcare) at a 1:2000 dilution in TBST supplemented with 1 % milk powder for 1 h. Membranes were washed once in TBST and once in TBS.

    Techniques: Expressing, SDS Page, Western Blot, Negative Control, Transformation Assay, Plasmid Preparation

    Steroid receptor associated and regulated protein and HSPB7 copy number changes across different malignancies. Heat map demonstrates mean gene‐level estimates for SRARP and HSPB7 in 35 TCGA datasets and TCGA Pan‐Cancer dataset. The GISTIC2_thersholded method was utilized to measure SRARP and HSPB7 gene‐level copy number changes. Next, significance levels for copy number changes between cancer types and their matched normal tissues were calculated using the Kruskal–Wallis test. For each TCGA dataset, the name of dataset, SRARP and HSPB7 copy number changes (copy no.), and sample size are shown. * P

    Journal: Molecular Oncology

    Article Title: SRARP and HSPB7 are epigenetically regulated gene pairs that function as tumor suppressors and predict clinical outcome in malignancies

    doi: 10.1002/1878-0261.12195

    Figure Lengend Snippet: Steroid receptor associated and regulated protein and HSPB7 copy number changes across different malignancies. Heat map demonstrates mean gene‐level estimates for SRARP and HSPB7 in 35 TCGA datasets and TCGA Pan‐Cancer dataset. The GISTIC2_thersholded method was utilized to measure SRARP and HSPB7 gene‐level copy number changes. Next, significance levels for copy number changes between cancer types and their matched normal tissues were calculated using the Kruskal–Wallis test. For each TCGA dataset, the name of dataset, SRARP and HSPB7 copy number changes (copy no.), and sample size are shown. * P

    Article Snippet: TaqMan Gene Expression Assays (Life Technologies) for SRARP (assay ID: Hs00698851_m1), HSPB7 (assay ID: Hs00205296_m1), and AR (Hs00171172_m1) were applied for qRT‐PCR as instructed by the manufacturer.

    Techniques:

    Gene expression data using qRT‐PCR for the epigenetic regulation of SRARP and HSPB7 in cancer cells and the effect of AR inactivation on SRARP. (A) Heat map showing relative expression of SRARP and HSPB7 following DNA demethylation using AZA in cancer cell lines. P value is the significance of fold change between AZA‐treated and control cells using a t ‐test. All fold changes are significant at a P

    Journal: Molecular Oncology

    Article Title: SRARP and HSPB7 are epigenetically regulated gene pairs that function as tumor suppressors and predict clinical outcome in malignancies

    doi: 10.1002/1878-0261.12195

    Figure Lengend Snippet: Gene expression data using qRT‐PCR for the epigenetic regulation of SRARP and HSPB7 in cancer cells and the effect of AR inactivation on SRARP. (A) Heat map showing relative expression of SRARP and HSPB7 following DNA demethylation using AZA in cancer cell lines. P value is the significance of fold change between AZA‐treated and control cells using a t ‐test. All fold changes are significant at a P

    Article Snippet: TaqMan Gene Expression Assays (Life Technologies) for SRARP (assay ID: Hs00698851_m1), HSPB7 (assay ID: Hs00205296_m1), and AR (Hs00171172_m1) were applied for qRT‐PCR as instructed by the manufacturer.

    Techniques: Expressing, Quantitative RT-PCR

    Differential gene expression and relative promoter methylation of SRARP and HSPB7 . (A) Heat map to show SRARP and HSPB7 differential expression values in eighteen tumor types and their matched normal tissues. Differential gene expression values were calculated as follows: log2 (RPKM+1)‐transformed median values of tumor ‐ log2 (RPKM+1) of normal. P values for tumor and normal pairs were calculated using the Mann–Whitney U ‐test. * P

    Journal: Molecular Oncology

    Article Title: SRARP and HSPB7 are epigenetically regulated gene pairs that function as tumor suppressors and predict clinical outcome in malignancies

    doi: 10.1002/1878-0261.12195

    Figure Lengend Snippet: Differential gene expression and relative promoter methylation of SRARP and HSPB7 . (A) Heat map to show SRARP and HSPB7 differential expression values in eighteen tumor types and their matched normal tissues. Differential gene expression values were calculated as follows: log2 (RPKM+1)‐transformed median values of tumor ‐ log2 (RPKM+1) of normal. P values for tumor and normal pairs were calculated using the Mann–Whitney U ‐test. * P

    Article Snippet: TaqMan Gene Expression Assays (Life Technologies) for SRARP (assay ID: Hs00698851_m1), HSPB7 (assay ID: Hs00205296_m1), and AR (Hs00171172_m1) were applied for qRT‐PCR as instructed by the manufacturer.

    Techniques: Expressing, Methylation, Transformation Assay, MANN-WHITNEY

    Copy number correlations of SRARP and HSPB7 genes in malignancies. Heat maps and correlation coefficients for SRARP ( C1orf64 ) and HSPB7 copy numbers are shown across malignancies of multiple tissue origins. Copy number data were analyzed using the ONCOMINE database. Each heat map depicts the correlation pattern between SRARP and HSPB7 across one of the datasets. Study name, tissue of origin, and sample size are shown for each dataset. Red and blue colors denote higher and lower DNA copies, respectively.

    Journal: Molecular Oncology

    Article Title: SRARP and HSPB7 are epigenetically regulated gene pairs that function as tumor suppressors and predict clinical outcome in malignancies

    doi: 10.1002/1878-0261.12195

    Figure Lengend Snippet: Copy number correlations of SRARP and HSPB7 genes in malignancies. Heat maps and correlation coefficients for SRARP ( C1orf64 ) and HSPB7 copy numbers are shown across malignancies of multiple tissue origins. Copy number data were analyzed using the ONCOMINE database. Each heat map depicts the correlation pattern between SRARP and HSPB7 across one of the datasets. Study name, tissue of origin, and sample size are shown for each dataset. Red and blue colors denote higher and lower DNA copies, respectively.

    Article Snippet: TaqMan Gene Expression Assays (Life Technologies) for SRARP (assay ID: Hs00698851_m1), HSPB7 (assay ID: Hs00205296_m1), and AR (Hs00171172_m1) were applied for qRT‐PCR as instructed by the manufacturer.

    Techniques:

    Chromosomal location of SRARP and HSPB7 genes and the predicted motifs of HSPB7 protein. (A) Gene‐based display and the distance between SRARP ( C1orf64 ) and HSPB7 genes on chromosome 1p36.13 using VEGA database. SRARP and HSPB7 are shown on the sense and antisense strands, respectively, and the known isoforms for HSPB7 are demonstrated. CLCNKA ‐003 start site on the sense strand is also depicted. (B and C) HSPB7 protein sequence was analyzed using scansite 3 software to identify regulatory motifs. Motif scan was carried out with high stringency to detect the best 0.2% of all sites. (B) Predicted motifs and their sequence score, percentile, sequence of motif, and surface accessibility are shown. (C) Predicted motif sites and a HSP20 domain within the HSPB7 sequence. AAs: amino acids.

    Journal: Molecular Oncology

    Article Title: SRARP and HSPB7 are epigenetically regulated gene pairs that function as tumor suppressors and predict clinical outcome in malignancies

    doi: 10.1002/1878-0261.12195

    Figure Lengend Snippet: Chromosomal location of SRARP and HSPB7 genes and the predicted motifs of HSPB7 protein. (A) Gene‐based display and the distance between SRARP ( C1orf64 ) and HSPB7 genes on chromosome 1p36.13 using VEGA database. SRARP and HSPB7 are shown on the sense and antisense strands, respectively, and the known isoforms for HSPB7 are demonstrated. CLCNKA ‐003 start site on the sense strand is also depicted. (B and C) HSPB7 protein sequence was analyzed using scansite 3 software to identify regulatory motifs. Motif scan was carried out with high stringency to detect the best 0.2% of all sites. (B) Predicted motifs and their sequence score, percentile, sequence of motif, and surface accessibility are shown. (C) Predicted motif sites and a HSP20 domain within the HSPB7 sequence. AAs: amino acids.

    Article Snippet: TaqMan Gene Expression Assays (Life Technologies) for SRARP (assay ID: Hs00698851_m1), HSPB7 (assay ID: Hs00205296_m1), and AR (Hs00171172_m1) were applied for qRT‐PCR as instructed by the manufacturer.

    Techniques: Sequencing, Software, Atomic Absorption Spectroscopy

    Effects of SRARP and HSPB7 overexpression on colony formation and signaling pathways. (A) Western blotting to assess SRARP and HSPB7 overexpression in A549 cells following transfections. CTL‐VEC: control vector; SRARP+: SRARP overexpression; HSPB7+: HSPB7 overexpression. (B) Clonogenic assays in stably transfected A549 cells containing SRARP (SRARP+), HSPB7 (HSPB7+), or CTL‐VEC. Experiments were carried out in four replicates. ANOVA with Dunnett's post hoc test was applied to calculate the statistical significance. * P

    Journal: Molecular Oncology

    Article Title: SRARP and HSPB7 are epigenetically regulated gene pairs that function as tumor suppressors and predict clinical outcome in malignancies

    doi: 10.1002/1878-0261.12195

    Figure Lengend Snippet: Effects of SRARP and HSPB7 overexpression on colony formation and signaling pathways. (A) Western blotting to assess SRARP and HSPB7 overexpression in A549 cells following transfections. CTL‐VEC: control vector; SRARP+: SRARP overexpression; HSPB7+: HSPB7 overexpression. (B) Clonogenic assays in stably transfected A549 cells containing SRARP (SRARP+), HSPB7 (HSPB7+), or CTL‐VEC. Experiments were carried out in four replicates. ANOVA with Dunnett's post hoc test was applied to calculate the statistical significance. * P

    Article Snippet: TaqMan Gene Expression Assays (Life Technologies) for SRARP (assay ID: Hs00698851_m1), HSPB7 (assay ID: Hs00205296_m1), and AR (Hs00171172_m1) were applied for qRT‐PCR as instructed by the manufacturer.

    Techniques: Over Expression, Western Blot, Transfection, CTL Assay, Plasmid Preparation, Stable Transfection

    Association of SRARP and HSPB7 gene expression and copy numbers with survival in cancer patients and normal adjacent tissues using ICGC datasets. Survival analysis was performed using Kaplan–Meier curves and the log‐rank test to estimate the survival probability. (A) Survival analysis based on SRARP gene expression in ICGC cancer patients. (B) Survival analysis based on SRARP gene expression in ICGC normal adjacent tissues. (C) Survival analysis based on HSPB7 gene expression in ICGC cancer patients. (D) Survival analysis based on HSPB7 gene expression in ICGC normal adjacent tissues. (E) Survival analysis based on SRARP copy number in ICGC cancer patients. (F) Survival analysis based on HSPB7 copy number in ICGC cancer patients.

    Journal: Molecular Oncology

    Article Title: SRARP and HSPB7 are epigenetically regulated gene pairs that function as tumor suppressors and predict clinical outcome in malignancies

    doi: 10.1002/1878-0261.12195

    Figure Lengend Snippet: Association of SRARP and HSPB7 gene expression and copy numbers with survival in cancer patients and normal adjacent tissues using ICGC datasets. Survival analysis was performed using Kaplan–Meier curves and the log‐rank test to estimate the survival probability. (A) Survival analysis based on SRARP gene expression in ICGC cancer patients. (B) Survival analysis based on SRARP gene expression in ICGC normal adjacent tissues. (C) Survival analysis based on HSPB7 gene expression in ICGC cancer patients. (D) Survival analysis based on HSPB7 gene expression in ICGC normal adjacent tissues. (E) Survival analysis based on SRARP copy number in ICGC cancer patients. (F) Survival analysis based on HSPB7 copy number in ICGC cancer patients.

    Article Snippet: TaqMan Gene Expression Assays (Life Technologies) for SRARP (assay ID: Hs00698851_m1), HSPB7 (assay ID: Hs00205296_m1), and AR (Hs00171172_m1) were applied for qRT‐PCR as instructed by the manufacturer.

    Techniques: Expressing

    Association of SRARP and HSPB7 methylation and expression with survival in normal solid tissues using TCGA Pan‐Cancer datasets. Survival analysis was performed using Kaplan–Meier curves and the log‐rank test to estimate the survival probability. (A) Survival analysis based on SRARP DNA methylation levels in TCGA normal solid tissues. (B) Survival analysis based on SRARP exon expression in TCGA normal solid tissues. (C) Survival analysis based on SRARP gene expression in TCGA normal solid tissues. (D) Survival analysis based on HSPB7 DNA methylation levels in TCGA normal solid tissues. (E) Survival analysis based on HSPB7 exon expression in TCGA normal solid tissues. (F) Survival analysis based on HSPB7 gene expression in TCGA normal solid tissues.

    Journal: Molecular Oncology

    Article Title: SRARP and HSPB7 are epigenetically regulated gene pairs that function as tumor suppressors and predict clinical outcome in malignancies

    doi: 10.1002/1878-0261.12195

    Figure Lengend Snippet: Association of SRARP and HSPB7 methylation and expression with survival in normal solid tissues using TCGA Pan‐Cancer datasets. Survival analysis was performed using Kaplan–Meier curves and the log‐rank test to estimate the survival probability. (A) Survival analysis based on SRARP DNA methylation levels in TCGA normal solid tissues. (B) Survival analysis based on SRARP exon expression in TCGA normal solid tissues. (C) Survival analysis based on SRARP gene expression in TCGA normal solid tissues. (D) Survival analysis based on HSPB7 DNA methylation levels in TCGA normal solid tissues. (E) Survival analysis based on HSPB7 exon expression in TCGA normal solid tissues. (F) Survival analysis based on HSPB7 gene expression in TCGA normal solid tissues.

    Article Snippet: TaqMan Gene Expression Assays (Life Technologies) for SRARP (assay ID: Hs00698851_m1), HSPB7 (assay ID: Hs00205296_m1), and AR (Hs00171172_m1) were applied for qRT‐PCR as instructed by the manufacturer.

    Techniques: Methylation, Expressing, DNA Methylation Assay

    Association of SRARP and HSPB7 methylation, expressi on, and mutations with survival in primary tumors using TCGA Pan‐Cancer datasets. Survival analysis was performed using Kaplan–Meier curves and the log‐rank test to estimate the survival probability. (A) Survival analysis based on SRARP DNA methylation levels in TCGA primary tumors. (B) Survival analysis based on SRARP exon expression in TCGA primary tumors. (C) Survival analysis based on SRARP gene expression in TCGA primary tumors. (D) Survival analysis based on SRARP somatic mutations in TCGA primary tumors. (E) Survival analysis based on HSPB7 DNA methylation levels in TCGA primary tumors. (F) Survival analysis based on HSPB7 gene expression in TCGA primary tumors. * P

    Journal: Molecular Oncology

    Article Title: SRARP and HSPB7 are epigenetically regulated gene pairs that function as tumor suppressors and predict clinical outcome in malignancies

    doi: 10.1002/1878-0261.12195

    Figure Lengend Snippet: Association of SRARP and HSPB7 methylation, expressi on, and mutations with survival in primary tumors using TCGA Pan‐Cancer datasets. Survival analysis was performed using Kaplan–Meier curves and the log‐rank test to estimate the survival probability. (A) Survival analysis based on SRARP DNA methylation levels in TCGA primary tumors. (B) Survival analysis based on SRARP exon expression in TCGA primary tumors. (C) Survival analysis based on SRARP gene expression in TCGA primary tumors. (D) Survival analysis based on SRARP somatic mutations in TCGA primary tumors. (E) Survival analysis based on HSPB7 DNA methylation levels in TCGA primary tumors. (F) Survival analysis based on HSPB7 gene expression in TCGA primary tumors. * P

    Article Snippet: TaqMan Gene Expression Assays (Life Technologies) for SRARP (assay ID: Hs00698851_m1), HSPB7 (assay ID: Hs00205296_m1), and AR (Hs00171172_m1) were applied for qRT‐PCR as instructed by the manufacturer.

    Techniques: Methylation, DNA Methylation Assay, Expressing

    Effects of SRARP and HSPB7 overexpression on colony formation. (A) Western blotting to assess SRARP and HSPB7 overexpression in MDA‐MB‐231 cells following transfections. Fold change (RR) in each band density was measured relative to control in three replicate experiments. CTL‐VEC: control vector; SRARP+: SRARP overexpression; HSPB7 + : HSPB7 overexpression. (B) Clonogenic assays to assess colony formation in stably transfected MDA‐MB‐231 cells. A total of 1000 cells containing SRARP (SRARP+), HSPB7 (HSPB7 + ), or CTL‐VEC were cultured for 21 days in selection medium, and colonies with more than 50 cells were counted. Experiments were carried out in four replicates. ANOVA with Dunnett's post hoc test was applied to calculate the statistical significance. * P

    Journal: Molecular Oncology

    Article Title: SRARP and HSPB7 are epigenetically regulated gene pairs that function as tumor suppressors and predict clinical outcome in malignancies

    doi: 10.1002/1878-0261.12195

    Figure Lengend Snippet: Effects of SRARP and HSPB7 overexpression on colony formation. (A) Western blotting to assess SRARP and HSPB7 overexpression in MDA‐MB‐231 cells following transfections. Fold change (RR) in each band density was measured relative to control in three replicate experiments. CTL‐VEC: control vector; SRARP+: SRARP overexpression; HSPB7 + : HSPB7 overexpression. (B) Clonogenic assays to assess colony formation in stably transfected MDA‐MB‐231 cells. A total of 1000 cells containing SRARP (SRARP+), HSPB7 (HSPB7 + ), or CTL‐VEC were cultured for 21 days in selection medium, and colonies with more than 50 cells were counted. Experiments were carried out in four replicates. ANOVA with Dunnett's post hoc test was applied to calculate the statistical significance. * P

    Article Snippet: TaqMan Gene Expression Assays (Life Technologies) for SRARP (assay ID: Hs00698851_m1), HSPB7 (assay ID: Hs00205296_m1), and AR (Hs00171172_m1) were applied for qRT‐PCR as instructed by the manufacturer.

    Techniques: Over Expression, Western Blot, Multiple Displacement Amplification, Transfection, CTL Assay, Plasmid Preparation, Stable Transfection, Cell Culture, Selection

    Systemic levels of Hsp60 were similar in healthy individuals and patients with different forms of periodontitis. The concentration of Hsp60 in the serum was measured by two-site ELISA. The distribution of transformed Hsp60 concentrations in the serum of healthy subjects (H, n = 28) and patients diagnosed with chronic (ChP, n = 29) or aggressive (AgP, n = 30) periodontitis is visualised as box-and-whisker plots (SPSS)

    Journal: Cell Stress & Chaperones

    Article Title: Association between circulating levels of heat-shock protein 27 and aggressive periodontitis

    doi: 10.1007/s12192-018-0891-4

    Figure Lengend Snippet: Systemic levels of Hsp60 were similar in healthy individuals and patients with different forms of periodontitis. The concentration of Hsp60 in the serum was measured by two-site ELISA. The distribution of transformed Hsp60 concentrations in the serum of healthy subjects (H, n = 28) and patients diagnosed with chronic (ChP, n = 29) or aggressive (AgP, n = 30) periodontitis is visualised as box-and-whisker plots (SPSS)

    Article Snippet: Hsp60 in serum or recombinant Hsp60 protein (ATgen; 0–5 μg/ml) was captured with a mouse monoclonal anti-Hsp60 antibody (clone LK1, Stressgen; 1 μg/ml).

    Techniques: Concentration Assay, Enzyme-linked Immunosorbent Assay, Transformation Assay, Whisker Assay