heat-inactivated rabbit serum Coy Laboratory Search Results


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  • 97
    ATCC t denticola atcc 33520
    Western blots with T. <t>denticola</t> Tap1 antiserum (A), T. pallidum FlgE antiserum (B), and T. phagedenis FlaB antiserum (C). Lanes 1, JS97; lanes 2, AS98; lanes 3, wild type. The arrow in panel A indicates the Tap1 polypeptide band. In panel B, the polypeptide ladder is a typical pattern found in treponemal hook polypeptides that may be due to cross-linking. The significance of the minor band missing in lane 3 is unknown. Numbers at right of each panel represent molecular masses in kilodaltons.
    T Denticola Atcc 33520, supplied by ATCC, used in various techniques. Bioz Stars score: 97/100, based on 122 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/t denticola atcc 33520/product/ATCC
    Average 97 stars, based on 122 article reviews
    Price from $9.99 to $1999.99
    t denticola atcc 33520 - by Bioz Stars, 2020-09
    97/100 stars
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    99
    Millipore heat inactivated rabbit serum
    Western blots with T. <t>denticola</t> Tap1 antiserum (A), T. pallidum FlgE antiserum (B), and T. phagedenis FlaB antiserum (C). Lanes 1, JS97; lanes 2, AS98; lanes 3, wild type. The arrow in panel A indicates the Tap1 polypeptide band. In panel B, the polypeptide ladder is a typical pattern found in treponemal hook polypeptides that may be due to cross-linking. The significance of the minor band missing in lane 3 is unknown. Numbers at right of each panel represent molecular masses in kilodaltons.
    Heat Inactivated Rabbit Serum, supplied by Millipore, used in various techniques. Bioz Stars score: 99/100, based on 118 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/heat inactivated rabbit serum/product/Millipore
    Average 99 stars, based on 118 article reviews
    Price from $9.99 to $1999.99
    heat inactivated rabbit serum - by Bioz Stars, 2020-09
    99/100 stars
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    99
    ATCC t denticola atcc 35405
    Western blots with T. <t>denticola</t> Tap1 antiserum (A), T. pallidum FlgE antiserum (B), and T. phagedenis FlaB antiserum (C). Lanes 1, JS97; lanes 2, AS98; lanes 3, wild type. The arrow in panel A indicates the Tap1 polypeptide band. In panel B, the polypeptide ladder is a typical pattern found in treponemal hook polypeptides that may be due to cross-linking. The significance of the minor band missing in lane 3 is unknown. Numbers at right of each panel represent molecular masses in kilodaltons.
    T Denticola Atcc 35405, supplied by ATCC, used in various techniques. Bioz Stars score: 99/100, based on 671 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/t denticola atcc 35405/product/ATCC
    Average 99 stars, based on 671 article reviews
    Price from $9.99 to $1999.99
    t denticola atcc 35405 - by Bioz Stars, 2020-09
    99/100 stars
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    99
    Millipore puromycin
    Western blots with T. <t>denticola</t> Tap1 antiserum (A), T. pallidum FlgE antiserum (B), and T. phagedenis FlaB antiserum (C). Lanes 1, JS97; lanes 2, AS98; lanes 3, wild type. The arrow in panel A indicates the Tap1 polypeptide band. In panel B, the polypeptide ladder is a typical pattern found in treponemal hook polypeptides that may be due to cross-linking. The significance of the minor band missing in lane 3 is unknown. Numbers at right of each panel represent molecular masses in kilodaltons.
    Puromycin, supplied by Millipore, used in various techniques. Bioz Stars score: 99/100, based on 46434 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/puromycin/product/Millipore
    Average 99 stars, based on 46434 article reviews
    Price from $9.99 to $1999.99
    puromycin - by Bioz Stars, 2020-09
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    88
    Merck KGaA milli q ultrapurification system
    Western blots with T. <t>denticola</t> Tap1 antiserum (A), T. pallidum FlgE antiserum (B), and T. phagedenis FlaB antiserum (C). Lanes 1, JS97; lanes 2, AS98; lanes 3, wild type. The arrow in panel A indicates the Tap1 polypeptide band. In panel B, the polypeptide ladder is a typical pattern found in treponemal hook polypeptides that may be due to cross-linking. The significance of the minor band missing in lane 3 is unknown. Numbers at right of each panel represent molecular masses in kilodaltons.
    Milli Q Ultrapurification System, supplied by Merck KGaA, used in various techniques. Bioz Stars score: 88/100, based on 4 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/milli q ultrapurification system/product/Merck KGaA
    Average 88 stars, based on 4 article reviews
    Price from $9.99 to $1999.99
    milli q ultrapurification system - by Bioz Stars, 2020-09
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    85
    Becton Dickinson spirolate broth
    Western blots with T. <t>denticola</t> Tap1 antiserum (A), T. pallidum FlgE antiserum (B), and T. phagedenis FlaB antiserum (C). Lanes 1, JS97; lanes 2, AS98; lanes 3, wild type. The arrow in panel A indicates the Tap1 polypeptide band. In panel B, the polypeptide ladder is a typical pattern found in treponemal hook polypeptides that may be due to cross-linking. The significance of the minor band missing in lane 3 is unknown. Numbers at right of each panel represent molecular masses in kilodaltons.
    Spirolate Broth, supplied by Becton Dickinson, used in various techniques. Bioz Stars score: 85/100, based on 5 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/spirolate broth/product/Becton Dickinson
    Average 85 stars, based on 5 article reviews
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    spirolate broth - by Bioz Stars, 2020-09
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    94
    ATCC t vincentii atcc 35580
    Western blots with T. <t>denticola</t> Tap1 antiserum (A), T. pallidum FlgE antiserum (B), and T. phagedenis FlaB antiserum (C). Lanes 1, JS97; lanes 2, AS98; lanes 3, wild type. The arrow in panel A indicates the Tap1 polypeptide band. In panel B, the polypeptide ladder is a typical pattern found in treponemal hook polypeptides that may be due to cross-linking. The significance of the minor band missing in lane 3 is unknown. Numbers at right of each panel represent molecular masses in kilodaltons.
    T Vincentii Atcc 35580, supplied by ATCC, used in various techniques. Bioz Stars score: 94/100, based on 50 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/t vincentii atcc 35580/product/ATCC
    Average 94 stars, based on 50 article reviews
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    Image Search Results


    Western blots with T. denticola Tap1 antiserum (A), T. pallidum FlgE antiserum (B), and T. phagedenis FlaB antiserum (C). Lanes 1, JS97; lanes 2, AS98; lanes 3, wild type. The arrow in panel A indicates the Tap1 polypeptide band. In panel B, the polypeptide ladder is a typical pattern found in treponemal hook polypeptides that may be due to cross-linking. The significance of the minor band missing in lane 3 is unknown. Numbers at right of each panel represent molecular masses in kilodaltons.

    Journal: Journal of Bacteriology

    Article Title: Insertional Inactivation of Treponemadenticola tap1 Results in a Nonmotile Mutant with Elongated Flagellar Hooks

    doi:

    Figure Lengend Snippet: Western blots with T. denticola Tap1 antiserum (A), T. pallidum FlgE antiserum (B), and T. phagedenis FlaB antiserum (C). Lanes 1, JS97; lanes 2, AS98; lanes 3, wild type. The arrow in panel A indicates the Tap1 polypeptide band. In panel B, the polypeptide ladder is a typical pattern found in treponemal hook polypeptides that may be due to cross-linking. The significance of the minor band missing in lane 3 is unknown. Numbers at right of each panel represent molecular masses in kilodaltons.

    Article Snippet: T. denticola (ATCC 33520) was grown in new oral spirochete medium (NOS) with 10% heat-inactivated rabbit serum and 10 μg of cocarboxylase per ml at 36°C in an anaerobic chamber (Coy Laboratory Products, Inc., Grass Lake, Mich.) with an atmosphere of 85% nitrogen, 5% carbon dioxide, and 10% hydrogen.

    Techniques: Western Blot

    Diagram of the fla operon organization of T. denticola and 5′ upstream region. P fla indicates the approximate location of the fla operon promoter. The ermF-ermAM cassette is indicated above the operon and is shown where it is inserted in the Bgl II site for creating Tap1-deficient mutants. The locations of primer pairs used for RT-PCR are indicated by arrows and are represented as follows: 1, TDW8; 2, TDW9; 3, TDW12; 4, TDW5; 5, TDWFLGEF; 6, TDWFLGER. Sequences of the primers are given in Materials and Methods.

    Journal: Journal of Bacteriology

    Article Title: Insertional Inactivation of Treponemadenticola tap1 Results in a Nonmotile Mutant with Elongated Flagellar Hooks

    doi:

    Figure Lengend Snippet: Diagram of the fla operon organization of T. denticola and 5′ upstream region. P fla indicates the approximate location of the fla operon promoter. The ermF-ermAM cassette is indicated above the operon and is shown where it is inserted in the Bgl II site for creating Tap1-deficient mutants. The locations of primer pairs used for RT-PCR are indicated by arrows and are represented as follows: 1, TDW8; 2, TDW9; 3, TDW12; 4, TDW5; 5, TDWFLGEF; 6, TDWFLGER. Sequences of the primers are given in Materials and Methods.

    Article Snippet: T. denticola (ATCC 33520) was grown in new oral spirochete medium (NOS) with 10% heat-inactivated rabbit serum and 10 μg of cocarboxylase per ml at 36°C in an anaerobic chamber (Coy Laboratory Products, Inc., Grass Lake, Mich.) with an atmosphere of 85% nitrogen, 5% carbon dioxide, and 10% hydrogen.

    Techniques: Reverse Transcription Polymerase Chain Reaction

    Comparison of the T. denticola fla promoter sequence with promoter sequences from various spirochete motility genes and consensus sigma 28 sequences.

    Journal: Journal of Bacteriology

    Article Title: Insertional Inactivation of Treponemadenticola tap1 Results in a Nonmotile Mutant with Elongated Flagellar Hooks

    doi:

    Figure Lengend Snippet: Comparison of the T. denticola fla promoter sequence with promoter sequences from various spirochete motility genes and consensus sigma 28 sequences.

    Article Snippet: T. denticola (ATCC 33520) was grown in new oral spirochete medium (NOS) with 10% heat-inactivated rabbit serum and 10 μg of cocarboxylase per ml at 36°C in an anaerobic chamber (Coy Laboratory Products, Inc., Grass Lake, Mich.) with an atmosphere of 85% nitrogen, 5% carbon dioxide, and 10% hydrogen.

    Techniques: Sequencing

    Alignment of Tap1 amino acid sequences. (A) Identical amino acids from three treponemes are boxed and shaded with SHADYBOX, which reveals the conserved region near the carboxyl terminus. The dark inverted triangle indicates the location of the point of insertion for the erythromycin resistance cassette into the T. denticola tap1 gene to generate a Tap1-deficient mutant. (B) Alignment of the conserved C-terminal region of T. denticola Tap1 with FliK of S. typhimurium ). Identical amino acids are boxed and shaded as described above.

    Journal: Journal of Bacteriology

    Article Title: Insertional Inactivation of Treponemadenticola tap1 Results in a Nonmotile Mutant with Elongated Flagellar Hooks

    doi:

    Figure Lengend Snippet: Alignment of Tap1 amino acid sequences. (A) Identical amino acids from three treponemes are boxed and shaded with SHADYBOX, which reveals the conserved region near the carboxyl terminus. The dark inverted triangle indicates the location of the point of insertion for the erythromycin resistance cassette into the T. denticola tap1 gene to generate a Tap1-deficient mutant. (B) Alignment of the conserved C-terminal region of T. denticola Tap1 with FliK of S. typhimurium ). Identical amino acids are boxed and shaded as described above.

    Article Snippet: T. denticola (ATCC 33520) was grown in new oral spirochete medium (NOS) with 10% heat-inactivated rabbit serum and 10 μg of cocarboxylase per ml at 36°C in an anaerobic chamber (Coy Laboratory Products, Inc., Grass Lake, Mich.) with an atmosphere of 85% nitrogen, 5% carbon dioxide, and 10% hydrogen.

    Techniques: Mutagenesis

    T. denticola wild type (WT) and Tap1-deficient mutant JS97 after growth for 7 days on NOS plates containing 0.5% agarose. Approximately 0.1 μl was placed on the plate and incubated at 36°C in an anaerobic chamber.

    Journal: Journal of Bacteriology

    Article Title: Insertional Inactivation of Treponemadenticola tap1 Results in a Nonmotile Mutant with Elongated Flagellar Hooks

    doi:

    Figure Lengend Snippet: T. denticola wild type (WT) and Tap1-deficient mutant JS97 after growth for 7 days on NOS plates containing 0.5% agarose. Approximately 0.1 μl was placed on the plate and incubated at 36°C in an anaerobic chamber.

    Article Snippet: T. denticola (ATCC 33520) was grown in new oral spirochete medium (NOS) with 10% heat-inactivated rabbit serum and 10 μg of cocarboxylase per ml at 36°C in an anaerobic chamber (Coy Laboratory Products, Inc., Grass Lake, Mich.) with an atmosphere of 85% nitrogen, 5% carbon dioxide, and 10% hydrogen.

    Techniques: Mutagenesis, Incubation