hcv rna Search Results


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  • 81
    Thermo Fisher hcv rna
    Predicted values and experimental validation of the first step of the ratiometric approach. (a) Measured spectral transmittance (%) in the range of visible light (400–700 nm) for positive (solid blue line) and negative (solid purple line) RT-LAMP reaction solutions, each containing 0.7 mM of eriochrome black T (EBT) as the amplification indicator dye. Dashed lines correspond to normalized spectral responses for red (R), green (G), and blue (B) channels of an Exmor R CMOS sensor, a common sensor in cell phone cameras. (b–e) Analysis of the three possible RGB ratiometric combinations for positive and negative RT-LAMP reaction solutions. (b) The predicted RGB values and corresponding colors for positive and negative LAMP amplification reactions obtained by convoluting the transmittance spectrum and Exmor R spectral responses described in panel a. (c) The cropped and enlarged color images collected with an Apple iPhone 4S for positive and negative RT-LAMP reaction solutions containing 90 μM of EBT dye. (d) Predicted images and ratiometric values for positive and negative amplification reactions processed for each ratiometric combination, G/R, B/R, and G/B. (e) Experimental images and ratiometric values for positive and negative amplification reactions for each combination: G/R, B/R, and G/B. All experiments were performed with <t>HCV</t> <t>RNA</t> as template.
    Hcv Rna, supplied by Thermo Fisher, used in various techniques. Bioz Stars score: 81/100, based on 1098 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    Roche hcv ribonucleic acid rna
    The laboratory testing algorithm used for identifying hepatitis C virus and hepatitis B virus infection. HBV = hepatitis B virus, HBc = hepatitis B core; HBs = hepatitis B surface; <t>HCV</t> = hepatitis C virus; <t>RNA</t> = ribonucleic acid.
    Hcv Ribonucleic Acid Rna, supplied by Roche, used in various techniques. Bioz Stars score: 95/100, based on 224 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    Roche hcv ribonucleic acid rna cobas taqman hcv test
    The laboratory testing algorithm used for identifying hepatitis C virus and hepatitis B virus infection. HBV = hepatitis B virus, HBc = hepatitis B core; HBs = hepatitis B surface; <t>HCV</t> = hepatitis C virus; <t>RNA</t> = ribonucleic acid.
    Hcv Ribonucleic Acid Rna Cobas Taqman Hcv Test, supplied by Roche, used in various techniques. Bioz Stars score: 77/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    Roche qualitative hcv rna
    Preferences for <t>HCV</t> testing strategy. Half of respondents consider two-step testing strategy (see Figure 1 ) to be an optimal future testing approach in LMIC, while the other half prefer currently used two-step approach a . If one-step testing strategy is implemented, more than a half of respondents prefer to use HCV <t>RNA</t> test b
    Qualitative Hcv Rna, supplied by Roche, used in various techniques. Bioz Stars score: 99/100, based on 81 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    Roche positive hcv ribonucleic acid rna polymerase chain reaction
    Preferences for <t>HCV</t> testing strategy. Half of respondents consider two-step testing strategy (see Figure 1 ) to be an optimal future testing approach in LMIC, while the other half prefer currently used two-step approach a . If one-step testing strategy is implemented, more than a half of respondents prefer to use HCV <t>RNA</t> test b
    Positive Hcv Ribonucleic Acid Rna Polymerase Chain Reaction, supplied by Roche, used in various techniques. Bioz Stars score: 77/100, based on 2 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    Abbott Laboratories hcv rna
    Distribution of <t>HCV-RNA</t> (A) and HCVAg (B) by HCV genotype. Significant differences between median values were observed only between genotypes 1 and 3 for both HCV-RNA (p = 0.028) and HCVAg (p = 0.0098).
    Hcv Rna, supplied by Abbott Laboratories, used in various techniques. Bioz Stars score: 99/100, based on 479 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    PrimerDesign Inc hcv rna
    <t>HCV</t> <t>RNA</t> Load in Brain and Liver Tissue
    Hcv Rna, supplied by PrimerDesign Inc, used in various techniques. Bioz Stars score: 89/100, based on 51 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    Thermo Fisher optiquant hcv rna
    qRT-PCR of IFN-γ in chimpanzees infected with HEV or <t>HCV.</t> IFN-γ was tested by primer- and probe-specific TaqMan qPCR (see Table S1 in the supplemental material). Total <t>RNA</t> from liver biopsies was converted to cDNA, amplified, and tested
    Optiquant Hcv Rna, supplied by Thermo Fisher, used in various techniques. Bioz Stars score: 93/100, based on 24 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    Roche amplicor hcv rna
    Mean values of results (expressed as log 10 <t>HCV</t> <t>RNA</t> copies per milliliter of serum) determined by our RT-PCR protocol (ABI Prism 7700 assay), the bDNA assay and the NGI method are comparable. (A) Comparison of totals; differences in mean values are not significant (ns). The percentages of samples positive by each method are given in the box. (B) Comparison of mean values for groups of different genotypes; differences are not significant.
    Amplicor Hcv Rna, supplied by Roche, used in various techniques. Bioz Stars score: 78/100, based on 2 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    Abbott Laboratories hcv rna asr
    Mean values of results (expressed as log 10 <t>HCV</t> <t>RNA</t> copies per milliliter of serum) determined by our RT-PCR protocol (ABI Prism 7700 assay), the bDNA assay and the NGI method are comparable. (A) Comparison of totals; differences in mean values are not significant (ns). The percentages of samples positive by each method are given in the box. (B) Comparison of mean values for groups of different genotypes; differences are not significant.
    Hcv Rna Asr, supplied by Abbott Laboratories, used in various techniques. Bioz Stars score: 77/100, based on 2 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    Thermo Fisher armored hcv rna
    Age related decline in prevalence of hepatitis C antibody in infants who had <t>anti-HCV</t> detected in their initial blood sample according to whether their mothers were positive for both <t>HCV-RNA</t> and anti-HCV or only had anti-HCV.
    Armored Hcv Rna, supplied by Thermo Fisher, used in various techniques. Bioz Stars score: 77/100, based on 2 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    LabCorp hcv rna hcv rna
    ( a–d ) Force–distance curves for target <t>HCV</t> <t>RNA</t> (6.0 fM) were recorded within a 150 nm × 150 nm area with a lateral pixel size of 10 nm. The number in each pixel represents the percentage of getting the specific event. The diameter of the clusters was within 30–60 nm. The pixels with the red circle showed the mean value out of the range (22–42 pN). ( e ) Adhesion force map of a 900 nm × 900 nm area with a lateral pixel size of 20 nm, obtained using target HCV RNA (5.7 fM). Mean force values were categorized into four levels, indicated by different colors. Isolated pixels are marked with red circles and clusters with blue circles.
    Hcv Rna Hcv Rna, supplied by LabCorp, used in various techniques. Bioz Stars score: 76/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    PrimerDesign Inc hcv rna expression
    REV-ERB activation perturbs fatty acid metabolism. a REV-ERB agonist SR9009 perturbs metabolic pathways. Huh-7 cells were treated with SR9009 (20 µM) or vehicle control for 24 h and gene expression analysed by human genome microarray (biological replicates n = 3). Differentially expressed genes were assessed by KEGG pathway enrichment analysis. b Metabolic genes perturbed by SR9009. Relative expression of metabolic genes involved in lipogenesis, cholesterol and bile acid metabolism following SR9009 (20 µM) treatment. c REV-ERB agonist inhibits SCD promoter activity and protein expression. Huh-7 cells were treated with REV-ERB agonist SR9009 (20 µM) for 24 h and SCD promoter activity (mean ± S.E.M., n = 4, Kruskal–Wallis ANOVA with Dunn’s test), transcript levels and protein expression assessed by western blotting and mass spectrometric analysis. d demonstrate REV-ERB peaks in SCD1 promoter. Read densities for REV-ERBα tracks are represented by height on the y axis. e Overexpressing REV-ERBα inhibits SCD expression. <t>HCV</t> A2-Luc replicon cells were transfected with an increasing dose of REV-ERBα expression plasmid. Cell lysates were collected 48 h post-transfection and assessed for SCD expression, together with housekeeping GAPDH by western blotting. f Oleic acid partially restores anti-viral activity of REV-ERB agonist SR9009. HCV replicon cells were treated with SR9009 at 15 µM (left) or SCD inhibitor A939572 (SCDi) at 10 µM (right) alone or in combination with oleic acid (OA) at 100 µM. HCV <t>RNA</t> replication was monitored at 30 min intervals for 24 h. g A role for SCD in REV-ERB agonist inhibition of HCV replication. HCV replicon cells were transfected with CRISPRs targeting exons 2 and 3 of SCD or a scrambled guide RNA and 24 h later treated with SR9009 or SCD inhibitor A939572. SCD expression was assessed by western blotting and the dose of REV-ERB agonist or SCD inhibitor required to inhibit HCV RNA replication by 50% (IC 50 ) in control or knock-down (KD) cells determined (mean ± S.E.M., n = 5, Mann–Whitney statistical test)
    Hcv Rna Expression, supplied by PrimerDesign Inc, used in various techniques. Bioz Stars score: 91/100, based on 12 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    Chiron Corporation quantiplex hcv rna kit
    REV-ERB activation perturbs fatty acid metabolism. a REV-ERB agonist SR9009 perturbs metabolic pathways. Huh-7 cells were treated with SR9009 (20 µM) or vehicle control for 24 h and gene expression analysed by human genome microarray (biological replicates n = 3). Differentially expressed genes were assessed by KEGG pathway enrichment analysis. b Metabolic genes perturbed by SR9009. Relative expression of metabolic genes involved in lipogenesis, cholesterol and bile acid metabolism following SR9009 (20 µM) treatment. c REV-ERB agonist inhibits SCD promoter activity and protein expression. Huh-7 cells were treated with REV-ERB agonist SR9009 (20 µM) for 24 h and SCD promoter activity (mean ± S.E.M., n = 4, Kruskal–Wallis ANOVA with Dunn’s test), transcript levels and protein expression assessed by western blotting and mass spectrometric analysis. d demonstrate REV-ERB peaks in SCD1 promoter. Read densities for REV-ERBα tracks are represented by height on the y axis. e Overexpressing REV-ERBα inhibits SCD expression. <t>HCV</t> A2-Luc replicon cells were transfected with an increasing dose of REV-ERBα expression plasmid. Cell lysates were collected 48 h post-transfection and assessed for SCD expression, together with housekeeping GAPDH by western blotting. f Oleic acid partially restores anti-viral activity of REV-ERB agonist SR9009. HCV replicon cells were treated with SR9009 at 15 µM (left) or SCD inhibitor A939572 (SCDi) at 10 µM (right) alone or in combination with oleic acid (OA) at 100 µM. HCV <t>RNA</t> replication was monitored at 30 min intervals for 24 h. g A role for SCD in REV-ERB agonist inhibition of HCV replication. HCV replicon cells were transfected with CRISPRs targeting exons 2 and 3 of SCD or a scrambled guide RNA and 24 h later treated with SR9009 or SCD inhibitor A939572. SCD expression was assessed by western blotting and the dose of REV-ERB agonist or SCD inhibitor required to inhibit HCV RNA replication by 50% (IC 50 ) in control or knock-down (KD) cells determined (mean ± S.E.M., n = 5, Mann–Whitney statistical test)
    Quantiplex Hcv Rna Kit, supplied by Chiron Corporation, used in various techniques. Bioz Stars score: 78/100, based on 4 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    Bayer AG versant hcv rna assay
    Comparison of the dynamic ranges of the three quantitative <t>HCV</t> <t>RNA</t> assays expressed in HCV international units per milliliter. The dynamic range of each assay is represented by the shaded portion of the corresponding horizontal bar. The 800,000-HCV-IU/ml threshold for tailoring the duration of therapy proposed by Pawlotsky et al. is indicated by the heavy vertical line.
    Versant Hcv Rna Assay, supplied by Bayer AG, used in various techniques. Bioz Stars score: 80/100, based on 9 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    Thermo Fisher hcv 1a armored rna
    Association between IFN Response, <t>HCV</t> <t>RNA</t> Levels, and Effect on Host Gene Expression in HCV-Infected Mice Intrahepatic HCV RNA levels were determined by quantitative RT-PCR as described in Materials and Methods. Total number of genes showing increased expression in HCV-infected mice relative to donor-matched uninfected mice is shown in gray bars, while the percent of induced genes that are IFN regulated is shown using black triangles.
    Hcv 1a Armored Rna, supplied by Thermo Fisher, used in various techniques. Bioz Stars score: 81/100, based on 23 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    Shanghai Kehua hcv rna diagnostic kit
    Association between IFN Response, <t>HCV</t> <t>RNA</t> Levels, and Effect on Host Gene Expression in HCV-Infected Mice Intrahepatic HCV RNA levels were determined by quantitative RT-PCR as described in Materials and Methods. Total number of genes showing increased expression in HCV-infected mice relative to donor-matched uninfected mice is shown in gray bars, while the percent of induced genes that are IFN regulated is shown using black triangles.
    Hcv Rna Diagnostic Kit, supplied by Shanghai Kehua, used in various techniques. Bioz Stars score: 86/100, based on 6 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    Abbott Laboratories abbott realtime hcv rna
    Association between IFN Response, <t>HCV</t> <t>RNA</t> Levels, and Effect on Host Gene Expression in HCV-Infected Mice Intrahepatic HCV RNA levels were determined by quantitative RT-PCR as described in Materials and Methods. Total number of genes showing increased expression in HCV-infected mice relative to donor-matched uninfected mice is shown in gray bars, while the percent of induced genes that are IFN regulated is shown using black triangles.
    Abbott Realtime Hcv Rna, supplied by Abbott Laboratories, used in various techniques. Bioz Stars score: 79/100, based on 3 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    Roche hcv rna test hcv rna
    Index values of <t>anti-HCV</t> immunoglobulin G in the positive and negative HCV <t>RNA</t> test groups. The index value of anti-HCV was > 10 in 99.1% of those in the positive HCV RNA group but only 19.1% of those in the negative HCV RNA group.
    Hcv Rna Test Hcv Rna, supplied by Roche, used in various techniques. Bioz Stars score: 77/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    Becton Dickinson hcv rna
    NK cells release soluble factor(s) to suppress expression of <t>HCV</t> <t>RNA</t> and NS5A protein in human hepatocytes. (a) Co-culture of NK cells with HCV JFH1-infected Huh7.5.1 cells. At day 4 postinfection, Huh7.5.1 cells in the low compartment of a 24-well transwell co-culture system were co-cultured with primary NK (PNK) or NK92 cells that were added to the top compartment of the co-culture system. (b) Effect of NK SN on HCV JFH1 RNA expression. JFH1-infected Huh7.5.1 cells (day 4 postinfection) were cultured in media conditioned with or without NK SN (PNK or NK92) for 48 h. Total cellular RNA extracted from the hepatic cells was subjected to real-time RT-PCR for HCV and GAPDH RNA quantification. The data are expressed as HCV RNA levels relative to the control (% of control, no NK cells on the top compartment or without treatment with NK SN, which is defined as 100%). The results shown are mean ± SD of triplicate cultures, representative of three separate experiments (*, P
    Hcv Rna, supplied by Becton Dickinson, used in various techniques. Bioz Stars score: 90/100, based on 51 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    Janssen hcv rna
    NK cells release soluble factor(s) to suppress expression of <t>HCV</t> <t>RNA</t> and NS5A protein in human hepatocytes. (a) Co-culture of NK cells with HCV JFH1-infected Huh7.5.1 cells. At day 4 postinfection, Huh7.5.1 cells in the low compartment of a 24-well transwell co-culture system were co-cultured with primary NK (PNK) or NK92 cells that were added to the top compartment of the co-culture system. (b) Effect of NK SN on HCV JFH1 RNA expression. JFH1-infected Huh7.5.1 cells (day 4 postinfection) were cultured in media conditioned with or without NK SN (PNK or NK92) for 48 h. Total cellular RNA extracted from the hepatic cells was subjected to real-time RT-PCR for HCV and GAPDH RNA quantification. The data are expressed as HCV RNA levels relative to the control (% of control, no NK cells on the top compartment or without treatment with NK SN, which is defined as 100%). The results shown are mean ± SD of triplicate cultures, representative of three separate experiments (*, P
    Hcv Rna, supplied by Janssen, used in various techniques. Bioz Stars score: 92/100, based on 6 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    srl inc hcv rna
    NK cells release soluble factor(s) to suppress expression of <t>HCV</t> <t>RNA</t> and NS5A protein in human hepatocytes. (a) Co-culture of NK cells with HCV JFH1-infected Huh7.5.1 cells. At day 4 postinfection, Huh7.5.1 cells in the low compartment of a 24-well transwell co-culture system were co-cultured with primary NK (PNK) or NK92 cells that were added to the top compartment of the co-culture system. (b) Effect of NK SN on HCV JFH1 RNA expression. JFH1-infected Huh7.5.1 cells (day 4 postinfection) were cultured in media conditioned with or without NK SN (PNK or NK92) for 48 h. Total cellular RNA extracted from the hepatic cells was subjected to real-time RT-PCR for HCV and GAPDH RNA quantification. The data are expressed as HCV RNA levels relative to the control (% of control, no NK cells on the top compartment or without treatment with NK SN, which is defined as 100%). The results shown are mean ± SD of triplicate cultures, representative of three separate experiments (*, P
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    Gen-Probe hcv rna
    NK cells release soluble factor(s) to suppress expression of <t>HCV</t> <t>RNA</t> and NS5A protein in human hepatocytes. (a) Co-culture of NK cells with HCV JFH1-infected Huh7.5.1 cells. At day 4 postinfection, Huh7.5.1 cells in the low compartment of a 24-well transwell co-culture system were co-cultured with primary NK (PNK) or NK92 cells that were added to the top compartment of the co-culture system. (b) Effect of NK SN on HCV JFH1 RNA expression. JFH1-infected Huh7.5.1 cells (day 4 postinfection) were cultured in media conditioned with or without NK SN (PNK or NK92) for 48 h. Total cellular RNA extracted from the hepatic cells was subjected to real-time RT-PCR for HCV and GAPDH RNA quantification. The data are expressed as HCV RNA levels relative to the control (% of control, no NK cells on the top compartment or without treatment with NK SN, which is defined as 100%). The results shown are mean ± SD of triplicate cultures, representative of three separate experiments (*, P
    Hcv Rna, supplied by Gen-Probe, used in various techniques. Bioz Stars score: 97/100, based on 56 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    SeraCare hcv rna
    Accuracy across the linear range of the CAP/CTM <t>HCV</t> test, v2.0, measured in EDTA plasma (a) and serum (b) matrices. Open diamonds, genotype 1 clinical specimen; black diamonds, armored <t>RNA,</t> genotype 1; light gray diamonds, 3rd WHO HCV RNA international
    Hcv Rna, supplied by SeraCare, used in various techniques. Bioz Stars score: 89/100, based on 10 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    Amazon hcv rna
    Accuracy across the linear range of the CAP/CTM <t>HCV</t> test, v2.0, measured in EDTA plasma (a) and serum (b) matrices. Open diamonds, genotype 1 clinical specimen; black diamonds, armored <t>RNA,</t> genotype 1; light gray diamonds, 3rd WHO HCV RNA international
    Hcv Rna, supplied by Amazon, used in various techniques. Bioz Stars score: 97/100, based on 7 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    Covance hcv rna
    Accuracy across the linear range of the CAP/CTM <t>HCV</t> test, v2.0, measured in EDTA plasma (a) and serum (b) matrices. Open diamonds, genotype 1 clinical specimen; black diamonds, armored <t>RNA,</t> genotype 1; light gray diamonds, 3rd WHO HCV RNA international
    Hcv Rna, supplied by Covance, used in various techniques. Bioz Stars score: 94/100, based on 5 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    Luxor Furniture hcv rna
    Accuracy across the linear range of the CAP/CTM <t>HCV</t> test, v2.0, measured in EDTA plasma (a) and serum (b) matrices. Open diamonds, genotype 1 clinical specimen; black diamonds, armored <t>RNA,</t> genotype 1; light gray diamonds, 3rd WHO HCV RNA international
    Hcv Rna, supplied by Luxor Furniture, used in various techniques. Bioz Stars score: 97/100, based on 3 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    Image Search Results


    Predicted values and experimental validation of the first step of the ratiometric approach. (a) Measured spectral transmittance (%) in the range of visible light (400–700 nm) for positive (solid blue line) and negative (solid purple line) RT-LAMP reaction solutions, each containing 0.7 mM of eriochrome black T (EBT) as the amplification indicator dye. Dashed lines correspond to normalized spectral responses for red (R), green (G), and blue (B) channels of an Exmor R CMOS sensor, a common sensor in cell phone cameras. (b–e) Analysis of the three possible RGB ratiometric combinations for positive and negative RT-LAMP reaction solutions. (b) The predicted RGB values and corresponding colors for positive and negative LAMP amplification reactions obtained by convoluting the transmittance spectrum and Exmor R spectral responses described in panel a. (c) The cropped and enlarged color images collected with an Apple iPhone 4S for positive and negative RT-LAMP reaction solutions containing 90 μM of EBT dye. (d) Predicted images and ratiometric values for positive and negative amplification reactions processed for each ratiometric combination, G/R, B/R, and G/B. (e) Experimental images and ratiometric values for positive and negative amplification reactions for each combination: G/R, B/R, and G/B. All experiments were performed with HCV RNA as template.

    Journal: ACS Nano

    Article Title: Reading Out Single-Molecule Digital RNA and DNA Isothermal Amplification in Nanoliter Volumes with Unmodified Camera Phones

    doi: 10.1021/acsnano.5b07338

    Figure Lengend Snippet: Predicted values and experimental validation of the first step of the ratiometric approach. (a) Measured spectral transmittance (%) in the range of visible light (400–700 nm) for positive (solid blue line) and negative (solid purple line) RT-LAMP reaction solutions, each containing 0.7 mM of eriochrome black T (EBT) as the amplification indicator dye. Dashed lines correspond to normalized spectral responses for red (R), green (G), and blue (B) channels of an Exmor R CMOS sensor, a common sensor in cell phone cameras. (b–e) Analysis of the three possible RGB ratiometric combinations for positive and negative RT-LAMP reaction solutions. (b) The predicted RGB values and corresponding colors for positive and negative LAMP amplification reactions obtained by convoluting the transmittance spectrum and Exmor R spectral responses described in panel a. (c) The cropped and enlarged color images collected with an Apple iPhone 4S for positive and negative RT-LAMP reaction solutions containing 90 μM of EBT dye. (d) Predicted images and ratiometric values for positive and negative amplification reactions processed for each ratiometric combination, G/R, B/R, and G/B. (e) Experimental images and ratiometric values for positive and negative amplification reactions for each combination: G/R, B/R, and G/B. All experiments were performed with HCV RNA as template.

    Article Snippet: HCV Viral RNA Purification from AcroMetrix HCV High Control A total of 200 μL of plasma containing HCV RNA (viral load estimate provided by AcroMetrix: 1.1–3.5 IU/mL) was extracted using the QIAamp Viral RNA Mini Kit (QIAGEN, Inc., Valencia, CA, USA) according to the manufacturer’s instructions.

    Techniques: Amplification

    Validation of the robustness of the G/R ratiometric approach to different hardware (cell phone cameras) and lighting conditions. (a–g) Enlarged and cropped color images (top two rows of each individual panel) captured by an unmodified cell phone camera from positive (+) and negative (−) RT-LAMP reactions at 2-fold increases in EBT concentration from 10.9 μM to 1.4 mM (1 = 0.011 mM; 2 = 0.022 mM; 3 = 0.044 mM, 4 = 0.088 mM, 5 = 0.175 mM; 6 = 0.35 mM; 7 = 0.7 mM; 8 = 1.4 mM). Positive wells are blue and negative wells are purple. After G/R ratiometric processing (bottom two rows of each individual panel), negative wells are black. Regions I, II, III in each panel indicate the effect of dye concentration: (II) acceptable concentration range for visualization (green regions); (I) concentrations too low for visualization (white regions); and (III) concentrations too high for visualization (red regions). (a–d) Images captured by four common cell phones under fluorescent light: (a) Apple iPhone 4S, (b) HTC inspire 4G, (c) Motorola Moto G, and (d) Nokia 808 PureView. (e–g) Images captured by an Apple iPhone 4S under three additional light conditions: (e) incandescent light, (f) direct sunlight, and (g) indirect sunlight. All experiments were performed with HCV RNA as a clinically relevant target. All images were acquired with unmodified cell phone cameras. Detailed information for the G/R ratiometric process (Figure S2) and additional cell phone camera images (Figure S3) are provided in the Supporting Information .

    Journal: ACS Nano

    Article Title: Reading Out Single-Molecule Digital RNA and DNA Isothermal Amplification in Nanoliter Volumes with Unmodified Camera Phones

    doi: 10.1021/acsnano.5b07338

    Figure Lengend Snippet: Validation of the robustness of the G/R ratiometric approach to different hardware (cell phone cameras) and lighting conditions. (a–g) Enlarged and cropped color images (top two rows of each individual panel) captured by an unmodified cell phone camera from positive (+) and negative (−) RT-LAMP reactions at 2-fold increases in EBT concentration from 10.9 μM to 1.4 mM (1 = 0.011 mM; 2 = 0.022 mM; 3 = 0.044 mM, 4 = 0.088 mM, 5 = 0.175 mM; 6 = 0.35 mM; 7 = 0.7 mM; 8 = 1.4 mM). Positive wells are blue and negative wells are purple. After G/R ratiometric processing (bottom two rows of each individual panel), negative wells are black. Regions I, II, III in each panel indicate the effect of dye concentration: (II) acceptable concentration range for visualization (green regions); (I) concentrations too low for visualization (white regions); and (III) concentrations too high for visualization (red regions). (a–d) Images captured by four common cell phones under fluorescent light: (a) Apple iPhone 4S, (b) HTC inspire 4G, (c) Motorola Moto G, and (d) Nokia 808 PureView. (e–g) Images captured by an Apple iPhone 4S under three additional light conditions: (e) incandescent light, (f) direct sunlight, and (g) indirect sunlight. All experiments were performed with HCV RNA as a clinically relevant target. All images were acquired with unmodified cell phone cameras. Detailed information for the G/R ratiometric process (Figure S2) and additional cell phone camera images (Figure S3) are provided in the Supporting Information .

    Article Snippet: HCV Viral RNA Purification from AcroMetrix HCV High Control A total of 200 μL of plasma containing HCV RNA (viral load estimate provided by AcroMetrix: 1.1–3.5 IU/mL) was extracted using the QIAamp Viral RNA Mini Kit (QIAGEN, Inc., Valencia, CA, USA) according to the manufacturer’s instructions.

    Techniques: Concentration Assay

    Experimental validation of two-step SlipChip devices for single molecule counting with an unmodified cell phone camera. (a) A flow-chart of detection of single molecules in two-step SlipChip: (i) 5 nL amplification wells are loaded with amplification reaction solution (RXN) and 9.5 nL detection wells are loaded with indicator dye (DYE). (ii) After amplification, a slip is performed and the RXN and DYE wells are combined. (iii) Immediately after mixing, positive reaction solutions become blue, while negative reactions remain purple. The readout is imaged by an unmodified cell phone camera. (iv) Ratiometric image processing (G/R process) provides a single binary result (positive or negative). (b) Stereoscope image of the device before the amplification and readout wells are merged (arrow designates direction of slip). (c) Stereoscope, (d) cell phone camera and (e) fluorescent images after the device is slipped and the wells are merged. (f) Stereoscope and (g) cell phone camera images after G/R image processing. (h) Correlation between fluorescence counts and cell phone (G/R processed) counts. Colors were enhanced in figure panels b–d, and f for clarity of publication; raw images were used in all ratiometric analyses. In these experiments, HCV RNA was amplified by dRT-LAMP.

    Journal: ACS Nano

    Article Title: Reading Out Single-Molecule Digital RNA and DNA Isothermal Amplification in Nanoliter Volumes with Unmodified Camera Phones

    doi: 10.1021/acsnano.5b07338

    Figure Lengend Snippet: Experimental validation of two-step SlipChip devices for single molecule counting with an unmodified cell phone camera. (a) A flow-chart of detection of single molecules in two-step SlipChip: (i) 5 nL amplification wells are loaded with amplification reaction solution (RXN) and 9.5 nL detection wells are loaded with indicator dye (DYE). (ii) After amplification, a slip is performed and the RXN and DYE wells are combined. (iii) Immediately after mixing, positive reaction solutions become blue, while negative reactions remain purple. The readout is imaged by an unmodified cell phone camera. (iv) Ratiometric image processing (G/R process) provides a single binary result (positive or negative). (b) Stereoscope image of the device before the amplification and readout wells are merged (arrow designates direction of slip). (c) Stereoscope, (d) cell phone camera and (e) fluorescent images after the device is slipped and the wells are merged. (f) Stereoscope and (g) cell phone camera images after G/R image processing. (h) Correlation between fluorescence counts and cell phone (G/R processed) counts. Colors were enhanced in figure panels b–d, and f for clarity of publication; raw images were used in all ratiometric analyses. In these experiments, HCV RNA was amplified by dRT-LAMP.

    Article Snippet: HCV Viral RNA Purification from AcroMetrix HCV High Control A total of 200 μL of plasma containing HCV RNA (viral load estimate provided by AcroMetrix: 1.1–3.5 IU/mL) was extracted using the QIAamp Viral RNA Mini Kit (QIAGEN, Inc., Valencia, CA, USA) according to the manufacturer’s instructions.

    Techniques: Single Molecule Counting, Flow Cytometry, Amplification, Fluorescence

    The laboratory testing algorithm used for identifying hepatitis C virus and hepatitis B virus infection. HBV = hepatitis B virus, HBc = hepatitis B core; HBs = hepatitis B surface; HCV = hepatitis C virus; RNA = ribonucleic acid.

    Journal: Annals of Family Medicine

    Article Title: Detecting Hepatitis B and C by Combined Public Health and Primary Care Birth Cohort Testing

    doi: 10.1370/afm.2166

    Figure Lengend Snippet: The laboratory testing algorithm used for identifying hepatitis C virus and hepatitis B virus infection. HBV = hepatitis B virus, HBc = hepatitis B core; HBs = hepatitis B surface; HCV = hepatitis C virus; RNA = ribonucleic acid.

    Article Snippet: When a screening test was positive, subsequent tests included the following: HCV ribonucleic acid (RNA) (COBAS, Ampliprep/COBAS, Taqman HCV Quantitative Test, version 2.0, Roche Diagnostics) and/or immunoblot (Mikrogen), a hepatitis B surface antigen (HBsAg) test, and an antihepatitis B surface (anti-HBs) test (HBsAg II and Anti-HBs, Roche Diagnostics) (see ).

    Techniques: Infection

    Serum HCV RNA levels (LIU/mL) during and after interferon-free treatment in the present study EOT, end of the treatment response.

    Journal: Oncotarget

    Article Title: Interferon-free treatment for patients with chronic hepatitis C and autoimmune liver disease: higher SVR rates with special precautions for deterioration of autoimmune hepatitis

    doi: 10.18632/oncotarget.24391

    Figure Lengend Snippet: Serum HCV RNA levels (LIU/mL) during and after interferon-free treatment in the present study EOT, end of the treatment response.

    Article Snippet: Measurement of HCV RNA HCV RNA was measured by COBAS TaqMan HCV assay version 2.0 (Roche Diagnostics, Tokyo, Japan), with a lower limit of quantification of 15 IU/mL.

    Techniques:

    Longitudinal virological results following discordant or inconsistent TMA results at week 12 or week 20 of treatment Patients included in this analysis were those who: (left panel) had HCV RNA-positive results at baseline, had TMA-discordant (+/-) results at week 12, and subsequent virological results available at weeks 20 OR, (right panel) had HCV RNA-positive results at week 12, had TMA-discordant (+/-) results at week 20, and subsequent virological results available at week 24. Positive samples (+) were defined as those that were PCR-positive or TMA-positive (singly or in duplicate). Negative samples (-) were defined as those that were TMA-negative (singly or in duplicate). TMA-discordant or inconsistent results at both weeks 12 and 20 were more likely to be followed by TMA-negative than positive results (19 negative vs 2 positive, p

    Journal: Hepatology (Baltimore, Md.)

    Article Title: Interpretation of Positive TMA Test Results from PCR-Negative Samples Obtained after Treatment of Chronic Hepatitis C

    doi: 10.1002/hep.22487

    Figure Lengend Snippet: Longitudinal virological results following discordant or inconsistent TMA results at week 12 or week 20 of treatment Patients included in this analysis were those who: (left panel) had HCV RNA-positive results at baseline, had TMA-discordant (+/-) results at week 12, and subsequent virological results available at weeks 20 OR, (right panel) had HCV RNA-positive results at week 12, had TMA-discordant (+/-) results at week 20, and subsequent virological results available at week 24. Positive samples (+) were defined as those that were PCR-positive or TMA-positive (singly or in duplicate). Negative samples (-) were defined as those that were TMA-negative (singly or in duplicate). TMA-discordant or inconsistent results at both weeks 12 and 20 were more likely to be followed by TMA-negative than positive results (19 negative vs 2 positive, p

    Article Snippet: Subjects with detectable HCV RNA at week 20 by PCR were deemed to be nonresponders to peginterferon/ribavirin and treatment was discontinued at week 24.

    Techniques: Polymerase Chain Reaction

    Long-term FU with liver elasticity measurement using Fibroscan and testings of HCV-RNA with a highly sensitive method in patients with a sustained virological response. The values of two patients with positive HCV-RNA at FU, indicative of occult infection, are shown with filled circles. EOT, end of treatment; FU, follow-up; HCV, hepatitis C virus.

    Journal: European Journal of Gastroenterology & Hepatology

    Article Title: Long-term follow-up after cure from chronic hepatitis C virus infection shows occult hepatitis and a risk of hepatocellular carcinoma in noncirrhotic patients

    doi: 10.1097/MEG.0000000000001316

    Figure Lengend Snippet: Long-term FU with liver elasticity measurement using Fibroscan and testings of HCV-RNA with a highly sensitive method in patients with a sustained virological response. The values of two patients with positive HCV-RNA at FU, indicative of occult infection, are shown with filled circles. EOT, end of treatment; FU, follow-up; HCV, hepatitis C virus.

    Article Snippet: HCV-RNA detection At FU, all patients were screened for HCV-RNA by PCR using the routine method of the MagNA Pure LC system/COBAS TaqMan HCV test (Roche Diagnostics, Mannheim, Germany), with a sensitivity of 15 IU/ml for the HCV WHO Standard.

    Techniques: Infection

    The transition rate of HCV RNA negativity with time.

    Journal: World Journal of Gastroenterology : WJG

    Article Title: Pegylated interferon plus ribavirin for genotype Ib chronic hepatitis C in Japan

    doi: 10.3748/wjg.14.7225

    Figure Lengend Snippet: The transition rate of HCV RNA negativity with time.

    Article Snippet: HCV RNA levels were measured by quantitative RT-PCR (Amplicor, Roche Diagnostic Systems, CA, USA).

    Techniques:

    The distribution of timing of HCV RNA undetectable after the initiation of treatment at weeks 2, 4, 8, and 12 in comparison with TaqMan and qualitative Amplicor assays . SVR, sustained virological response.

    Journal: BMC Gastroenterology

    Article Title: Excellent superiority and specificity of COBAS TaqMan HCV assay in an early viral kinetic change during pegylated interferon alpha-2b plus ribavirin treatment

    doi: 10.1186/1471-230X-10-38

    Figure Lengend Snippet: The distribution of timing of HCV RNA undetectable after the initiation of treatment at weeks 2, 4, 8, and 12 in comparison with TaqMan and qualitative Amplicor assays . SVR, sustained virological response.

    Article Snippet: Determination of HCV RNA level During the treatment period (the initial 12 weeks: at week 2, week 4, week 8, and week 12), we retrospectively determined serum HCV RNA level by both COBAS TaqMan HCV assay (TaqMan) (Roche Diagnostics) and COBAS Amplicor HCV Monitor Test v2.0 using the 10-fold dilution method (Amplicor 10-fold method) (Roche Diagnostics) in each patient.

    Techniques:

    Frequency of Phenotypic Resistance Profiles in Patients who Relapse by Prior Response and Subtype in Phase 3 Studies (includes the T12/PR arm of ADVANCE and pooled TVR arms of REALIZE). Relapse was defined as HCV RNA > 25 IU/mL during follow-up after

    Journal: PLoS ONE

    Article Title: Hepatitis C Viral Evolution in Genotype 1 Treatment-Na?ve and Treatment-Experienced Patients Receiving Telaprevir-Based Therapy in Clinical Trials

    doi: 10.1371/journal.pone.0034372

    Figure Lengend Snippet: Frequency of Phenotypic Resistance Profiles in Patients who Relapse by Prior Response and Subtype in Phase 3 Studies (includes the T12/PR arm of ADVANCE and pooled TVR arms of REALIZE). Relapse was defined as HCV RNA > 25 IU/mL during follow-up after

    Article Snippet: HCV RNA Quantitation and Subtyping Plasma HCV RNA levels were determined using the Roche COBAS TaqMan® HCV/HPS assay (Version 2.0) for Phase 3 trials.

    Techniques:

    Phenotypic Resistance Profiles in Patients Who Did Not Achieve SVR with a TVR-based Regimen. Data from ADVANCE include only the T12PR arm and data from REALIZE include pooled TVR arms. Higher-level resistance (red) is defined as > 25-fold increase in IC 50 and lower-level resistance (yellow) is defined as 3- to 25-fold increase in IC 50 from wild-type. Grey (n/a) indicates patients with no sequence data available due to HCV RNA levels below the LOD of the sequencing assay or lost-to-follow-up.

    Journal: PLoS ONE

    Article Title: Hepatitis C Viral Evolution in Genotype 1 Treatment-Na?ve and Treatment-Experienced Patients Receiving Telaprevir-Based Therapy in Clinical Trials

    doi: 10.1371/journal.pone.0034372

    Figure Lengend Snippet: Phenotypic Resistance Profiles in Patients Who Did Not Achieve SVR with a TVR-based Regimen. Data from ADVANCE include only the T12PR arm and data from REALIZE include pooled TVR arms. Higher-level resistance (red) is defined as > 25-fold increase in IC 50 and lower-level resistance (yellow) is defined as 3- to 25-fold increase in IC 50 from wild-type. Grey (n/a) indicates patients with no sequence data available due to HCV RNA levels below the LOD of the sequencing assay or lost-to-follow-up.

    Article Snippet: HCV RNA Quantitation and Subtyping Plasma HCV RNA levels were determined using the Roche COBAS TaqMan® HCV/HPS assay (Version 2.0) for Phase 3 trials.

    Techniques: Sequencing

    Treatment Outcome in Patients from Phase 3 Telaprevir Studies. Data from ADVANCE includes only the T12PR arm and data from REALIZE includes pooled TVR arms. ‘Other’ includes patients with missing SVR assessment and patients with HCV RNA > 25 IU/mL at last study dose but who did not have viral breakthrough. ‘Relapse’ here is calculated using a denominator of total number of patients, and so differs from a relapse rate calculated in Figure 8 which uses patients with undetectable HCV RNA at the end of treatment. ‘SVR’ rates here are calculated as in the INCIVEK USPI, which utilized the last recorded HCV RNA assessment; in case of missing data, the last HCV RNA assessment from week 12 of follow-up onward was used. For the determination of SVR and relapse rates, the lower limit of quantification (

    Journal: PLoS ONE

    Article Title: Hepatitis C Viral Evolution in Genotype 1 Treatment-Na?ve and Treatment-Experienced Patients Receiving Telaprevir-Based Therapy in Clinical Trials

    doi: 10.1371/journal.pone.0034372

    Figure Lengend Snippet: Treatment Outcome in Patients from Phase 3 Telaprevir Studies. Data from ADVANCE includes only the T12PR arm and data from REALIZE includes pooled TVR arms. ‘Other’ includes patients with missing SVR assessment and patients with HCV RNA > 25 IU/mL at last study dose but who did not have viral breakthrough. ‘Relapse’ here is calculated using a denominator of total number of patients, and so differs from a relapse rate calculated in Figure 8 which uses patients with undetectable HCV RNA at the end of treatment. ‘SVR’ rates here are calculated as in the INCIVEK USPI, which utilized the last recorded HCV RNA assessment; in case of missing data, the last HCV RNA assessment from week 12 of follow-up onward was used. For the determination of SVR and relapse rates, the lower limit of quantification (

    Article Snippet: HCV RNA Quantitation and Subtyping Plasma HCV RNA levels were determined using the Roche COBAS TaqMan® HCV/HPS assay (Version 2.0) for Phase 3 trials.

    Techniques:

    Distribution of IL-18 polymorphism gene. A: reveals the percentage of gene allele distribution in HCV patients. B: the percentage of C/C in responders (32 patients) compared to relapsers groups (0 patients) (35.2% versus 0%, respectively), and the percentage of A/C in relapsers (5 patients) compared to responders (59 patients) (100% versus 64.8% respectively), with no statistical significant between these groups, P = 0.16. C: PCR digestion for −607 polymorphism IL-18 gene allele: Agarose gel electrophoresis analysis of rs1946518 polymorphism in IL-18 gene. CC genotype produced only in 154 bp band and AC genotype produced 3 bands 154, 125 and 28 bp, respectively. M line represents the 50 bp molecular gene ruler DNA ladder used as a marker and 250 bp as a reference band (28 bp band not appear).

    Journal: Journal of Clinical and Experimental Hepatology

    Article Title: Effect of Sofosbuvir Plus Daclatasvir in Hepatitis C Virus Genotype-4 Patients: Promising Effect on Liver Fibrosis

    doi: 10.1016/j.jceh.2017.06.006

    Figure Lengend Snippet: Distribution of IL-18 polymorphism gene. A: reveals the percentage of gene allele distribution in HCV patients. B: the percentage of C/C in responders (32 patients) compared to relapsers groups (0 patients) (35.2% versus 0%, respectively), and the percentage of A/C in relapsers (5 patients) compared to responders (59 patients) (100% versus 64.8% respectively), with no statistical significant between these groups, P = 0.16. C: PCR digestion for −607 polymorphism IL-18 gene allele: Agarose gel electrophoresis analysis of rs1946518 polymorphism in IL-18 gene. CC genotype produced only in 154 bp band and AC genotype produced 3 bands 154, 125 and 28 bp, respectively. M line represents the 50 bp molecular gene ruler DNA ladder used as a marker and 250 bp as a reference band (28 bp band not appear).

    Article Snippet: HCV-RNA PCR was done at 4 weeks as well as at 12 weeks after the end of treatment.

    Techniques: Polymerase Chain Reaction, Agarose Gel Electrophoresis, Produced, Marker

    Courses of acute HCV infection in five subjects after accidental needlestick exposure to HCV-positive blood. HCV RNA values were expressed as log GEs per milliliter of serum. sALT activity was expressed in U per liter. Total bilirubin levels are expressed as mg/dl and

    Journal: The Journal of Experimental Medicine

    Article Title: Determinants of Viral Clearance and Persistence during Acute Hepatitis C Virus Infection

    doi:

    Figure Lengend Snippet: Courses of acute HCV infection in five subjects after accidental needlestick exposure to HCV-positive blood. HCV RNA values were expressed as log GEs per milliliter of serum. sALT activity was expressed in U per liter. Total bilirubin levels are expressed as mg/dl and

    Article Snippet: HCV RNA Detection HCV RNA was detected using the reverse transcription (RT)-PCR with primers targeting the 5′ noncoding region of the HCV genome (HCV-Monitor Amplification kit; Roche Diagnostics Version 2.0) following the manufacturer's instructions.

    Techniques: Infection, Activity Assay

    Mean HCV RNA reduction according to IP-10 in HCV genotype 1 with favorable IL28B genotype.

    Journal: PLoS ONE

    Article Title: Response Prediction in Chronic Hepatitis C by Assessment of IP-10 and IL28B-Related Single Nucleotide Polymorphisms

    doi: 10.1371/journal.pone.0017232

    Figure Lengend Snippet: Mean HCV RNA reduction according to IP-10 in HCV genotype 1 with favorable IL28B genotype.

    Article Snippet: HCV RNA Quantification HCV RNA was determined by RT-PCR using Cobas Amplicor HCV Monitor version 2.0 (Roche Diagnostics, Branchburg, NJ), and quantified on days 0, 1, 4, 7, 8, 15, 22, 29, at end of treatment, and 24 months after the completion of treatment.

    Techniques:

    Preferences for HCV testing strategy. Half of respondents consider two-step testing strategy (see Figure 1 ) to be an optimal future testing approach in LMIC, while the other half prefer currently used two-step approach a . If one-step testing strategy is implemented, more than a half of respondents prefer to use HCV RNA test b

    Journal: BMC Infectious Diseases

    Article Title: Optimising diagnosis of viraemic hepatitis C infection: the development of a target product profile

    doi: 10.1186/s12879-017-2770-5

    Figure Lengend Snippet: Preferences for HCV testing strategy. Half of respondents consider two-step testing strategy (see Figure 1 ) to be an optimal future testing approach in LMIC, while the other half prefer currently used two-step approach a . If one-step testing strategy is implemented, more than a half of respondents prefer to use HCV RNA test b

    Article Snippet: Currently available commercial quantitative and qualitative HCV RNA or HCV cAg assays are focused on centralized laboratory infrastructure (e.g. Roche COBAS Taqman HCV assay, Abbott RealTime HCV assay, Hologic Aptima® HCV Quant Dx Assay) [ ].

    Techniques:

    Information about respondents. Professional profiles of 36 respondents to the HCV RNA and cAg TPPs

    Journal: BMC Infectious Diseases

    Article Title: Optimising diagnosis of viraemic hepatitis C infection: the development of a target product profile

    doi: 10.1186/s12879-017-2770-5

    Figure Lengend Snippet: Information about respondents. Professional profiles of 36 respondents to the HCV RNA and cAg TPPs

    Article Snippet: Currently available commercial quantitative and qualitative HCV RNA or HCV cAg assays are focused on centralized laboratory infrastructure (e.g. Roche COBAS Taqman HCV assay, Abbott RealTime HCV assay, Hologic Aptima® HCV Quant Dx Assay) [ ].

    Techniques:

    Preferred sensitivity and acceptable trade-offs between sensitivity and price. Over 50% of respondents see current price of HBV DNA, HCV RNA, and to a lesser extent HCV cAg, is seen as a major barrier to scale up the testing a . Half of respondents consider that 95% as an acceptable diagnostic sensitivity for a one-step diagnostic test whereas 43% prefer 98% b . Respondents were willing to pay more for a higher sensitivity and were willing to compromise on sensitivity for a cheap test c and d

    Journal: BMC Infectious Diseases

    Article Title: Optimising diagnosis of viraemic hepatitis C infection: the development of a target product profile

    doi: 10.1186/s12879-017-2770-5

    Figure Lengend Snippet: Preferred sensitivity and acceptable trade-offs between sensitivity and price. Over 50% of respondents see current price of HBV DNA, HCV RNA, and to a lesser extent HCV cAg, is seen as a major barrier to scale up the testing a . Half of respondents consider that 95% as an acceptable diagnostic sensitivity for a one-step diagnostic test whereas 43% prefer 98% b . Respondents were willing to pay more for a higher sensitivity and were willing to compromise on sensitivity for a cheap test c and d

    Article Snippet: Currently available commercial quantitative and qualitative HCV RNA or HCV cAg assays are focused on centralized laboratory infrastructure (e.g. Roche COBAS Taqman HCV assay, Abbott RealTime HCV assay, Hologic Aptima® HCV Quant Dx Assay) [ ].

    Techniques: Diagnostic Assay

    Preference for the HCV diagnostic test and the test of cure to be the same or different. Almost half of respondents preferred that the diagnostic test and test of cure be the same and about two thirds of these preferred that it be a decentralized (i.e. near-patient) HCV RNA test

    Journal: BMC Infectious Diseases

    Article Title: Optimising diagnosis of viraemic hepatitis C infection: the development of a target product profile

    doi: 10.1186/s12879-017-2770-5

    Figure Lengend Snippet: Preference for the HCV diagnostic test and the test of cure to be the same or different. Almost half of respondents preferred that the diagnostic test and test of cure be the same and about two thirds of these preferred that it be a decentralized (i.e. near-patient) HCV RNA test

    Article Snippet: Currently available commercial quantitative and qualitative HCV RNA or HCV cAg assays are focused on centralized laboratory infrastructure (e.g. Roche COBAS Taqman HCV assay, Abbott RealTime HCV assay, Hologic Aptima® HCV Quant Dx Assay) [ ].

    Techniques: Diagnostic Assay

    Distribution of HCV-RNA (A) and HCVAg (B) by HCV genotype. Significant differences between median values were observed only between genotypes 1 and 3 for both HCV-RNA (p = 0.028) and HCVAg (p = 0.0098).

    Journal: BMC Infectious Diseases

    Article Title: HCV core antigen and HCV-RNA in HIV/HCV co-infected patients with different HCV genotypes

    doi: 10.1186/1471-2334-14-222

    Figure Lengend Snippet: Distribution of HCV-RNA (A) and HCVAg (B) by HCV genotype. Significant differences between median values were observed only between genotypes 1 and 3 for both HCV-RNA (p = 0.028) and HCVAg (p = 0.0098).

    Article Snippet: The current standard for diagnosing an active HCV infection is the detection and quantification of HCV-RNA, also recommended to monitor the antiviral treatment.

    Techniques:

    Correlation between HCVAg and HCV-RNA on 315 samples from patients with HCV RNA > 12 IU/ml. Values are reported on a logarithmic scale and with different symbols according to genotype. The correlation coefficient (Spearman) was 0.869.

    Journal: BMC Infectious Diseases

    Article Title: HCV core antigen and HCV-RNA in HIV/HCV co-infected patients with different HCV genotypes

    doi: 10.1186/1471-2334-14-222

    Figure Lengend Snippet: Correlation between HCVAg and HCV-RNA on 315 samples from patients with HCV RNA > 12 IU/ml. Values are reported on a logarithmic scale and with different symbols according to genotype. The correlation coefficient (Spearman) was 0.869.

    Article Snippet: The current standard for diagnosing an active HCV infection is the detection and quantification of HCV-RNA, also recommended to monitor the antiviral treatment.

    Techniques:

    Monitoring profiles from three patients co-infected by HCV and HIV. The values of HCV-RNA and HCVAg are expressed on a logarithmic scale as IU/mL and fmol/L. respectively. The trends of the two parameters in the two patients described in Figure 3 A and B were almost identical whereas the patient in Figure 3 C showed a persistence of HCVAg positivity and a virological relapse on the following draw. Dotted line: HCVAg; solid line: HCV-RNA.

    Journal: BMC Infectious Diseases

    Article Title: HCV core antigen and HCV-RNA in HIV/HCV co-infected patients with different HCV genotypes

    doi: 10.1186/1471-2334-14-222

    Figure Lengend Snippet: Monitoring profiles from three patients co-infected by HCV and HIV. The values of HCV-RNA and HCVAg are expressed on a logarithmic scale as IU/mL and fmol/L. respectively. The trends of the two parameters in the two patients described in Figure 3 A and B were almost identical whereas the patient in Figure 3 C showed a persistence of HCVAg positivity and a virological relapse on the following draw. Dotted line: HCVAg; solid line: HCV-RNA.

    Article Snippet: The current standard for diagnosing an active HCV infection is the detection and quantification of HCV-RNA, also recommended to monitor the antiviral treatment.

    Techniques: Infection

    Kinetics of HCV RNA in different group of patients at different time points (week 0, 1, 2, 4, 8). The median HCV RNA level and relative range are reported with black circle for responder and with white circle for treatment-failing patients. Statistic “p” values are reported at each time points.

    Journal: Scientific Reports

    Article Title: Baseline and Breakthrough Resistance Mutations in HCV Patients Failing DAAs

    doi: 10.1038/s41598-017-15987-1

    Figure Lengend Snippet: Kinetics of HCV RNA in different group of patients at different time points (week 0, 1, 2, 4, 8). The median HCV RNA level and relative range are reported with black circle for responder and with white circle for treatment-failing patients. Statistic “p” values are reported at each time points.

    Article Snippet: Viral load decrease in responder and treatment-failing patients was compared at the baseline, 1, 2, 4 and 8 weeks, and after stopping with the using Abbott HCV-RNA assay (Abbott Park, Illinois, U.S.A.).

    Techniques:

    HCV RNA Load in Brain and Liver Tissue

    Journal: Gastroenterology

    Article Title: Hepatitis C Virus Infects the Endothelial Cells of the Blood-Brain Barrier

    doi: 10.1053/j.gastro.2011.11.028

    Figure Lengend Snippet: HCV RNA Load in Brain and Liver Tissue

    Article Snippet: Quantification of HCV RNA from matched samples of white and grey matter, cerebellum, medulla, liver, and plasma revealed that, in clinical samples with detectable brain HCV, the viral load was 1000- to 10,000-fold lower in brain compared with liver from the same subject.

    Techniques:

    HCV RNA and antigen expression in brain endothelial and hepatoma cells. hCMEC/D3 and Huh-7 cells were infected with HCVcc SA13/JFH for12hours, and unbound virus was removed by washing. ( A ) HCV RNA copies and ( B ) the frequency of NS5A-positive cells were

    Journal: Gastroenterology

    Article Title: Hepatitis C Virus Infects the Endothelial Cells of the Blood-Brain Barrier

    doi: 10.1053/j.gastro.2011.11.028

    Figure Lengend Snippet: HCV RNA and antigen expression in brain endothelial and hepatoma cells. hCMEC/D3 and Huh-7 cells were infected with HCVcc SA13/JFH for12hours, and unbound virus was removed by washing. ( A ) HCV RNA copies and ( B ) the frequency of NS5A-positive cells were

    Article Snippet: Quantification of HCV RNA from matched samples of white and grey matter, cerebellum, medulla, liver, and plasma revealed that, in clinical samples with detectable brain HCV, the viral load was 1000- to 10,000-fold lower in brain compared with liver from the same subject.

    Techniques: Expressing, Infection

    qRT-PCR of IFN-γ in chimpanzees infected with HEV or HCV. IFN-γ was tested by primer- and probe-specific TaqMan qPCR (see Table S1 in the supplemental material). Total RNA from liver biopsies was converted to cDNA, amplified, and tested

    Journal: Journal of Virology

    Article Title: Pathogenesis of Hepatitis E Virus and Hepatitis C Virus in Chimpanzees: Similarities and Differences ▿Pathogenesis of Hepatitis E Virus and Hepatitis C Virus in Chimpanzees: Similarities and Differences ▿ †

    doi: 10.1128/JVI.01205-10

    Figure Lengend Snippet: qRT-PCR of IFN-γ in chimpanzees infected with HEV or HCV. IFN-γ was tested by primer- and probe-specific TaqMan qPCR (see Table S1 in the supplemental material). Total RNA from liver biopsies was converted to cDNA, amplified, and tested

    Article Snippet: A quantity standard line representing a 6-log range was constructed with the OptiQuant HCV RNA (Acrometrix, Benicia, CA) nucleic acid test reference panel.

    Techniques: Quantitative RT-PCR, Infection, Real-time Polymerase Chain Reaction, Amplification

    Mean values of results (expressed as log 10 HCV RNA copies per milliliter of serum) determined by our RT-PCR protocol (ABI Prism 7700 assay), the bDNA assay and the NGI method are comparable. (A) Comparison of totals; differences in mean values are not significant (ns). The percentages of samples positive by each method are given in the box. (B) Comparison of mean values for groups of different genotypes; differences are not significant.

    Journal: Journal of Clinical Microbiology

    Article Title: High-Throughput Real-Time Reverse Transcription-PCR Quantitation of Hepatitis C Virus RNA

    doi:

    Figure Lengend Snippet: Mean values of results (expressed as log 10 HCV RNA copies per milliliter of serum) determined by our RT-PCR protocol (ABI Prism 7700 assay), the bDNA assay and the NGI method are comparable. (A) Comparison of totals; differences in mean values are not significant (ns). The percentages of samples positive by each method are given in the box. (B) Comparison of mean values for groups of different genotypes; differences are not significant.

    Article Snippet: Seventy-four patients were HCV RNA positive by a commercial quantitative HCV RNA test with a detection limit of 102 copies/ml (Amplicor HCV-RNA; Roche Molecular Systems) and five were negative.

    Techniques: Reverse Transcription Polymerase Chain Reaction

    Schematic representation of the transcription fragment from the 5′ NCR of HCV. Oligonucleotide primers C149 and C342 were used for amplification by RT-PCR. Primers C24 and C339 were hybridized to the RNA transcript to assess its integrity. FT275 is the dual-labeled probe. R, reporter; Q, quencher; nt, nucleotides.

    Journal: Journal of Clinical Microbiology

    Article Title: High-Throughput Real-Time Reverse Transcription-PCR Quantitation of Hepatitis C Virus RNA

    doi:

    Figure Lengend Snippet: Schematic representation of the transcription fragment from the 5′ NCR of HCV. Oligonucleotide primers C149 and C342 were used for amplification by RT-PCR. Primers C24 and C339 were hybridized to the RNA transcript to assess its integrity. FT275 is the dual-labeled probe. R, reporter; Q, quencher; nt, nucleotides.

    Article Snippet: Seventy-four patients were HCV RNA positive by a commercial quantitative HCV RNA test with a detection limit of 102 copies/ml (Amplicor HCV-RNA; Roche Molecular Systems) and five were negative.

    Techniques: Amplification, Reverse Transcription Polymerase Chain Reaction, Labeling

    Standard curve of the input RNA concentration in serial dilutions of the first reference standard versus C T . Each dot represents the result of triplicate PCR amplifications for each dilution. The stars represent the results of PCR amplification of samples containing unknown quantities of HCV RNA tested by this method. R was equal to 0.999, and the slope was −3.429.

    Journal: Journal of Clinical Microbiology

    Article Title: High-Throughput Real-Time Reverse Transcription-PCR Quantitation of Hepatitis C Virus RNA

    doi:

    Figure Lengend Snippet: Standard curve of the input RNA concentration in serial dilutions of the first reference standard versus C T . Each dot represents the result of triplicate PCR amplifications for each dilution. The stars represent the results of PCR amplification of samples containing unknown quantities of HCV RNA tested by this method. R was equal to 0.999, and the slope was −3.429.

    Article Snippet: Seventy-four patients were HCV RNA positive by a commercial quantitative HCV RNA test with a detection limit of 102 copies/ml (Amplicor HCV-RNA; Roche Molecular Systems) and five were negative.

    Techniques: Concentration Assay, Polymerase Chain Reaction, Amplification

    Age related decline in prevalence of hepatitis C antibody in infants who had anti-HCV detected in their initial blood sample according to whether their mothers were positive for both HCV-RNA and anti-HCV or only had anti-HCV.

    Journal: Journal of medical virology

    Article Title: Prospective Cohort Study of Mother-to-Infant Infection and Clearance of Hepatitis C in Rural Egyptian Villages

    doi: 10.1002/jmv.21480

    Figure Lengend Snippet: Age related decline in prevalence of hepatitis C antibody in infants who had anti-HCV detected in their initial blood sample according to whether their mothers were positive for both HCV-RNA and anti-HCV or only had anti-HCV.

    Article Snippet: Quantitative controls consisting of known copy numbers generated from Armored HCV RNA (Ambion, Austin, TX) were run with each reaction.

    Techniques:

    ( a–d ) Force–distance curves for target HCV RNA (6.0 fM) were recorded within a 150 nm × 150 nm area with a lateral pixel size of 10 nm. The number in each pixel represents the percentage of getting the specific event. The diameter of the clusters was within 30–60 nm. The pixels with the red circle showed the mean value out of the range (22–42 pN). ( e ) Adhesion force map of a 900 nm × 900 nm area with a lateral pixel size of 20 nm, obtained using target HCV RNA (5.7 fM). Mean force values were categorized into four levels, indicated by different colors. Isolated pixels are marked with red circles and clusters with blue circles.

    Journal: Nucleic Acids Research

    Article Title: Direct quantitative analysis of HCV RNA by atomic force microscopy without labeling or amplification

    doi: 10.1093/nar/gks953

    Figure Lengend Snippet: ( a–d ) Force–distance curves for target HCV RNA (6.0 fM) were recorded within a 150 nm × 150 nm area with a lateral pixel size of 10 nm. The number in each pixel represents the percentage of getting the specific event. The diameter of the clusters was within 30–60 nm. The pixels with the red circle showed the mean value out of the range (22–42 pN). ( e ) Adhesion force map of a 900 nm × 900 nm area with a lateral pixel size of 20 nm, obtained using target HCV RNA (5.7 fM). Mean force values were categorized into four levels, indicated by different colors. Isolated pixels are marked with red circles and clusters with blue circles.

    Article Snippet: Preparation of HCV RNA HCV RNA, which was single-stranded and 9.6 kb in length, was provided by the Laboratory Corporation of America (LabCorp).

    Techniques: Isolation

    Secondary structures of 5′ non-coding region (nucleotides 3–263) of HCV RNA ( http://rna.tbi.univie.ac.at/cgi-bin/RNAfold.cgi ). There are two major RNA secondary structures of which minimum free energy is ( a ) −98.95 kcal/mol and ( b ) −97.30 kcal/mol, respectively. Three sub-domains that were completely invariant among seven major genotypes of HCV RNA were indicated by blue (positions from 3 to 65), orange (positions from 178 to 199) and pink (positions 246 to 263).

    Journal: Nucleic Acids Research

    Article Title: Direct quantitative analysis of HCV RNA by atomic force microscopy without labeling or amplification

    doi: 10.1093/nar/gks953

    Figure Lengend Snippet: Secondary structures of 5′ non-coding region (nucleotides 3–263) of HCV RNA ( http://rna.tbi.univie.ac.at/cgi-bin/RNAfold.cgi ). There are two major RNA secondary structures of which minimum free energy is ( a ) −98.95 kcal/mol and ( b ) −97.30 kcal/mol, respectively. Three sub-domains that were completely invariant among seven major genotypes of HCV RNA were indicated by blue (positions from 3 to 65), orange (positions from 178 to 199) and pink (positions 246 to 263).

    Article Snippet: Preparation of HCV RNA HCV RNA, which was single-stranded and 9.6 kb in length, was provided by the Laboratory Corporation of America (LabCorp).

    Techniques:

    ( a ) Schematic of the experimental setup employed for the force mapping described in this study. Force measurements were recorded at a pulling rate of 0.70 μm/s between the detection DNA and the captured HCV RNA on the surface. The 18-mer detection DNA probe (green) complementary to nucleotides 246–263 of the HCV RNA (pink) was immobilized on the 27-acid dendron-modified AFM tip. The 60-mer capture probe DNA complementary to nucleotides 3–62 of the HCV RNA (blue) was immobilized on a 27-acid dendron-modified silicon substrate. Typical force–distance curve with ( b ) a single rupture and ( c ) multiple ruptures between the detection probe DNA and the complementary part of the HCV RNA.

    Journal: Nucleic Acids Research

    Article Title: Direct quantitative analysis of HCV RNA by atomic force microscopy without labeling or amplification

    doi: 10.1093/nar/gks953

    Figure Lengend Snippet: ( a ) Schematic of the experimental setup employed for the force mapping described in this study. Force measurements were recorded at a pulling rate of 0.70 μm/s between the detection DNA and the captured HCV RNA on the surface. The 18-mer detection DNA probe (green) complementary to nucleotides 246–263 of the HCV RNA (pink) was immobilized on the 27-acid dendron-modified AFM tip. The 60-mer capture probe DNA complementary to nucleotides 3–62 of the HCV RNA (blue) was immobilized on a 27-acid dendron-modified silicon substrate. Typical force–distance curve with ( b ) a single rupture and ( c ) multiple ruptures between the detection probe DNA and the complementary part of the HCV RNA.

    Article Snippet: Preparation of HCV RNA HCV RNA, which was single-stranded and 9.6 kb in length, was provided by the Laboratory Corporation of America (LabCorp).

    Techniques: Modification

    The histograms of the unbinding force ( a ) and the distance ( b ) derived from the force–distance curves of the interaction recorded on an area (150 nm × 150 nm) for a sample of 6.0 fM HCV RNA. The mean value of unbinding force was 32 ± 5 (the force value ± σ) pN (a) and the unbinding distance ranged from 8 nm to 70 nm (b). The probability of getting the specific event was 34%.

    Journal: Nucleic Acids Research

    Article Title: Direct quantitative analysis of HCV RNA by atomic force microscopy without labeling or amplification

    doi: 10.1093/nar/gks953

    Figure Lengend Snippet: The histograms of the unbinding force ( a ) and the distance ( b ) derived from the force–distance curves of the interaction recorded on an area (150 nm × 150 nm) for a sample of 6.0 fM HCV RNA. The mean value of unbinding force was 32 ± 5 (the force value ± σ) pN (a) and the unbinding distance ranged from 8 nm to 70 nm (b). The probability of getting the specific event was 34%.

    Article Snippet: Preparation of HCV RNA HCV RNA, which was single-stranded and 9.6 kb in length, was provided by the Laboratory Corporation of America (LabCorp).

    Techniques: Derivative Assay

    REV-ERB activation perturbs fatty acid metabolism. a REV-ERB agonist SR9009 perturbs metabolic pathways. Huh-7 cells were treated with SR9009 (20 µM) or vehicle control for 24 h and gene expression analysed by human genome microarray (biological replicates n = 3). Differentially expressed genes were assessed by KEGG pathway enrichment analysis. b Metabolic genes perturbed by SR9009. Relative expression of metabolic genes involved in lipogenesis, cholesterol and bile acid metabolism following SR9009 (20 µM) treatment. c REV-ERB agonist inhibits SCD promoter activity and protein expression. Huh-7 cells were treated with REV-ERB agonist SR9009 (20 µM) for 24 h and SCD promoter activity (mean ± S.E.M., n = 4, Kruskal–Wallis ANOVA with Dunn’s test), transcript levels and protein expression assessed by western blotting and mass spectrometric analysis. d demonstrate REV-ERB peaks in SCD1 promoter. Read densities for REV-ERBα tracks are represented by height on the y axis. e Overexpressing REV-ERBα inhibits SCD expression. HCV A2-Luc replicon cells were transfected with an increasing dose of REV-ERBα expression plasmid. Cell lysates were collected 48 h post-transfection and assessed for SCD expression, together with housekeeping GAPDH by western blotting. f Oleic acid partially restores anti-viral activity of REV-ERB agonist SR9009. HCV replicon cells were treated with SR9009 at 15 µM (left) or SCD inhibitor A939572 (SCDi) at 10 µM (right) alone or in combination with oleic acid (OA) at 100 µM. HCV RNA replication was monitored at 30 min intervals for 24 h. g A role for SCD in REV-ERB agonist inhibition of HCV replication. HCV replicon cells were transfected with CRISPRs targeting exons 2 and 3 of SCD or a scrambled guide RNA and 24 h later treated with SR9009 or SCD inhibitor A939572. SCD expression was assessed by western blotting and the dose of REV-ERB agonist or SCD inhibitor required to inhibit HCV RNA replication by 50% (IC 50 ) in control or knock-down (KD) cells determined (mean ± S.E.M., n = 5, Mann–Whitney statistical test)

    Journal: Nature Communications

    Article Title: The circadian clock components BMAL1 and REV-ERBα regulate flavivirus replication

    doi: 10.1038/s41467-019-08299-7

    Figure Lengend Snippet: REV-ERB activation perturbs fatty acid metabolism. a REV-ERB agonist SR9009 perturbs metabolic pathways. Huh-7 cells were treated with SR9009 (20 µM) or vehicle control for 24 h and gene expression analysed by human genome microarray (biological replicates n = 3). Differentially expressed genes were assessed by KEGG pathway enrichment analysis. b Metabolic genes perturbed by SR9009. Relative expression of metabolic genes involved in lipogenesis, cholesterol and bile acid metabolism following SR9009 (20 µM) treatment. c REV-ERB agonist inhibits SCD promoter activity and protein expression. Huh-7 cells were treated with REV-ERB agonist SR9009 (20 µM) for 24 h and SCD promoter activity (mean ± S.E.M., n = 4, Kruskal–Wallis ANOVA with Dunn’s test), transcript levels and protein expression assessed by western blotting and mass spectrometric analysis. d demonstrate REV-ERB peaks in SCD1 promoter. Read densities for REV-ERBα tracks are represented by height on the y axis. e Overexpressing REV-ERBα inhibits SCD expression. HCV A2-Luc replicon cells were transfected with an increasing dose of REV-ERBα expression plasmid. Cell lysates were collected 48 h post-transfection and assessed for SCD expression, together with housekeeping GAPDH by western blotting. f Oleic acid partially restores anti-viral activity of REV-ERB agonist SR9009. HCV replicon cells were treated with SR9009 at 15 µM (left) or SCD inhibitor A939572 (SCDi) at 10 µM (right) alone or in combination with oleic acid (OA) at 100 µM. HCV RNA replication was monitored at 30 min intervals for 24 h. g A role for SCD in REV-ERB agonist inhibition of HCV replication. HCV replicon cells were transfected with CRISPRs targeting exons 2 and 3 of SCD or a scrambled guide RNA and 24 h later treated with SR9009 or SCD inhibitor A939572. SCD expression was assessed by western blotting and the dose of REV-ERB agonist or SCD inhibitor required to inhibit HCV RNA replication by 50% (IC 50 ) in control or knock-down (KD) cells determined (mean ± S.E.M., n = 5, Mann–Whitney statistical test)

    Article Snippet: Purified total RNA samples were tested for HCV RNA expression (Primer Design Ltd, UK) in a qRT-PCR as per the manufacturer’s guidelines (CellsDirect Kit; Invitrogen, UK) in an MxPro-3000 PCR machine (Aligent, US).

    Techniques: Activation Assay, Expressing, Microarray, Activity Assay, Western Blot, Transfection, Plasmid Preparation, Inhibition, MANN-WHITNEY

    Model of circadian clock components regulating HCV, DENV and ZIKV replication. The circadian activator BMAL1 regulates HCV entry into hepatocytes through modulating viral receptors CD81 and claudin-1 expression. Activating REV-ERB with synthetic agonists or protein overexpression inhibits HCV, DENV or ZIKV RNA replication via modulating SCD and subsequent release of infectious particles

    Journal: Nature Communications

    Article Title: The circadian clock components BMAL1 and REV-ERBα regulate flavivirus replication

    doi: 10.1038/s41467-019-08299-7

    Figure Lengend Snippet: Model of circadian clock components regulating HCV, DENV and ZIKV replication. The circadian activator BMAL1 regulates HCV entry into hepatocytes through modulating viral receptors CD81 and claudin-1 expression. Activating REV-ERB with synthetic agonists or protein overexpression inhibits HCV, DENV or ZIKV RNA replication via modulating SCD and subsequent release of infectious particles

    Article Snippet: Purified total RNA samples were tested for HCV RNA expression (Primer Design Ltd, UK) in a qRT-PCR as per the manufacturer’s guidelines (CellsDirect Kit; Invitrogen, UK) in an MxPro-3000 PCR machine (Aligent, US).

    Techniques: Expressing, Over Expression

    HCV entry is circadian regulated. a Synchronisation of Huh-7 cells. Huh-7 cells were serum shocked and Bmal1 and Rev-Erbα mRNA measured by quantitative reverse transcription polymerase chain reaction (qRT-PCR) and expressed relative to circadian time (CT) 0. Data are the average of four independent experiments ( n = 4). b Circadian infection protocol. Synchronised Huh-7 were inoculated with HCVpp or HCVcc particles for 1 h and infectivity determined by luciferase assay or measuring the frequency of viral NS5A expressing cells 24 h later. c HCV entry shows a circadian pattern. Synchronised Huh-7 cells were inoculated with HCVpp at defined CTs and particle entry measured 24 h later and data expressed relative to CT0; mean ± S.E.M., n = 8, Kruskal–Wallis ANOVA with Dunn’s test. d HCV infection shows a circadian pattern. Synchronised Huh-7 were inoculated with HCVcc SA13/JFH-1 or J6/JFH-1 at defined CTs and the frequency of infected cells quantified 24 h later and data expressed relative to CT0; mean ± S.E.M., n = 4; Kruskal–Wallis ANOVA with Dunn’s test. e Circadian pattern of HCV receptors. Synchronised Huh-7 were lysed every 8 h, total RNA extracted and CD81 , claudin-1 and occludin mRNA levels together with the housekeeping GAPDH assessed by qPCR. Individual receptor transcripts were normalised to CT0. Representative of three independent experiments; n = 3, mean ± S.E.M. f BMAL1 regulates HCV entry receptors. Parental (WT) or Bmal1 KO Huh-7 lysates were assessed for BMAL1 and viral receptors CD81, claudin-1 and occludin expression together with housekeeping GAPDH by western blotting. Total RNA from parental or Bmal1 KO Huh-7 were extracted and mRNA of CD81, claudin-1, occludin and GAPDH measured by qRT-PCR. Data are expressed relative to parental cells (mean ± S.E.M., n = 3, Mann–Whitney test). g BMAL1 regulates HCV entry and infection. WT or Bmal1 KO Huh-7 were inoculated for 1 h with HCVpp or HCVcc SA13/JFH-1 and infection assessed after 24 h. Data are expressed relative to WT cells (mean ± S.E.M., n = 5, Mann–Whitney test). h BMAL1 regulates DENV and ZIKV infection. WT or Bmal1 KO Huh-7 were inoculated for 1 h with DENV or ZIKV and infection assessed after 24 h. Data are expressed relative to WT (mean ± S.E.M., n = 6 for DENV; n = 9 for ZIKV, Mann–Whitney test)

    Journal: Nature Communications

    Article Title: The circadian clock components BMAL1 and REV-ERBα regulate flavivirus replication

    doi: 10.1038/s41467-019-08299-7

    Figure Lengend Snippet: HCV entry is circadian regulated. a Synchronisation of Huh-7 cells. Huh-7 cells were serum shocked and Bmal1 and Rev-Erbα mRNA measured by quantitative reverse transcription polymerase chain reaction (qRT-PCR) and expressed relative to circadian time (CT) 0. Data are the average of four independent experiments ( n = 4). b Circadian infection protocol. Synchronised Huh-7 were inoculated with HCVpp or HCVcc particles for 1 h and infectivity determined by luciferase assay or measuring the frequency of viral NS5A expressing cells 24 h later. c HCV entry shows a circadian pattern. Synchronised Huh-7 cells were inoculated with HCVpp at defined CTs and particle entry measured 24 h later and data expressed relative to CT0; mean ± S.E.M., n = 8, Kruskal–Wallis ANOVA with Dunn’s test. d HCV infection shows a circadian pattern. Synchronised Huh-7 were inoculated with HCVcc SA13/JFH-1 or J6/JFH-1 at defined CTs and the frequency of infected cells quantified 24 h later and data expressed relative to CT0; mean ± S.E.M., n = 4; Kruskal–Wallis ANOVA with Dunn’s test. e Circadian pattern of HCV receptors. Synchronised Huh-7 were lysed every 8 h, total RNA extracted and CD81 , claudin-1 and occludin mRNA levels together with the housekeeping GAPDH assessed by qPCR. Individual receptor transcripts were normalised to CT0. Representative of three independent experiments; n = 3, mean ± S.E.M. f BMAL1 regulates HCV entry receptors. Parental (WT) or Bmal1 KO Huh-7 lysates were assessed for BMAL1 and viral receptors CD81, claudin-1 and occludin expression together with housekeeping GAPDH by western blotting. Total RNA from parental or Bmal1 KO Huh-7 were extracted and mRNA of CD81, claudin-1, occludin and GAPDH measured by qRT-PCR. Data are expressed relative to parental cells (mean ± S.E.M., n = 3, Mann–Whitney test). g BMAL1 regulates HCV entry and infection. WT or Bmal1 KO Huh-7 were inoculated for 1 h with HCVpp or HCVcc SA13/JFH-1 and infection assessed after 24 h. Data are expressed relative to WT cells (mean ± S.E.M., n = 5, Mann–Whitney test). h BMAL1 regulates DENV and ZIKV infection. WT or Bmal1 KO Huh-7 were inoculated for 1 h with DENV or ZIKV and infection assessed after 24 h. Data are expressed relative to WT (mean ± S.E.M., n = 6 for DENV; n = 9 for ZIKV, Mann–Whitney test)

    Article Snippet: Purified total RNA samples were tested for HCV RNA expression (Primer Design Ltd, UK) in a qRT-PCR as per the manufacturer’s guidelines (CellsDirect Kit; Invitrogen, UK) in an MxPro-3000 PCR machine (Aligent, US).

    Techniques: Reverse Transcription Polymerase Chain Reaction, Quantitative RT-PCR, Infection, Luciferase, Expressing, Real-time Polymerase Chain Reaction, Western Blot, MANN-WHITNEY

    REV-ERBα inhibits hepatitis C virus (HCV) RNA replication. a Silencing Rev-erbα increases HCV replication. Huh-7 cells supporting a HCV JFH-1-LUC replicon were transduced with lentivirus encoding sh Rev-erbα or control and silencing confirmed by measuring Rev-erbα mRNA and protein expression levels (mean ± S.E.M., n = 4, Mann–Whitney test). Densitometric analysis quantified REV-ERB in individual samples and was normalised to its own GAPDH loading control. HCV replication-dependent reporter activity was measured and expressed relative to control (shCtrl) cells (mean ± S.E.M., n = 6, Mann–Whitney test). b Anti-viral activity of SR9009 agonist is dependent on REV-ERB expression levels. sh Rev-eRbα and Ctrl HCV JFH-1 replicon cells described in ( a ) were treated with REV-ERB agonist SR9009 for 24 h, viral replication measured and the concentration of agonist required to inhibit viral replication by 50% defined (IC 50 ) (mean ± S.E.M., n = 3). c REV-ERBα overexpression inhibits HCV RNA replication. Huh-7 cells stably supporting a HCV JFH-1-LUC replicon were transfected with empty plasmid or vector expressing REV-ERBα and 48 h later protein expression assessed by western blotting and viral replication measured (mean ± S.E.M., n = 4, Mann–Whitney statistical test). Data are plotted relative to Ctrl untreated cells. d REV-ERB agonists cure HCV-infected cells. HCVcc SA13/JFH-1 infected Huh-7 cells were treated with increasing concentrations of REV-ERB agonists for 24 h and viral RNA or NS5A-expressing cells quantified and data expressed relative to Ctrl untreated cells. The experiment was performed in the presence of a neutralising anti-CD81 antibody to limit secondary rounds of infection (mean ± S.E.M., n = 3). e REV-ERB ligands inhibit the replication of diverse HCV genotypes. Huh-7 cells transiently supporting HCV sub-genomic replicons representing genotypes 1–3 were treated with the REV-ERB agonists SR9009 or GSK2667 and replication assessed 24 h later. The dose of agonist required to inhibit HCV RNA replication by 50% (IC 50 ) was determined for all viral genotypes (mean ± S.E.M., n = 3)

    Journal: Nature Communications

    Article Title: The circadian clock components BMAL1 and REV-ERBα regulate flavivirus replication

    doi: 10.1038/s41467-019-08299-7

    Figure Lengend Snippet: REV-ERBα inhibits hepatitis C virus (HCV) RNA replication. a Silencing Rev-erbα increases HCV replication. Huh-7 cells supporting a HCV JFH-1-LUC replicon were transduced with lentivirus encoding sh Rev-erbα or control and silencing confirmed by measuring Rev-erbα mRNA and protein expression levels (mean ± S.E.M., n = 4, Mann–Whitney test). Densitometric analysis quantified REV-ERB in individual samples and was normalised to its own GAPDH loading control. HCV replication-dependent reporter activity was measured and expressed relative to control (shCtrl) cells (mean ± S.E.M., n = 6, Mann–Whitney test). b Anti-viral activity of SR9009 agonist is dependent on REV-ERB expression levels. sh Rev-eRbα and Ctrl HCV JFH-1 replicon cells described in ( a ) were treated with REV-ERB agonist SR9009 for 24 h, viral replication measured and the concentration of agonist required to inhibit viral replication by 50% defined (IC 50 ) (mean ± S.E.M., n = 3). c REV-ERBα overexpression inhibits HCV RNA replication. Huh-7 cells stably supporting a HCV JFH-1-LUC replicon were transfected with empty plasmid or vector expressing REV-ERBα and 48 h later protein expression assessed by western blotting and viral replication measured (mean ± S.E.M., n = 4, Mann–Whitney statistical test). Data are plotted relative to Ctrl untreated cells. d REV-ERB agonists cure HCV-infected cells. HCVcc SA13/JFH-1 infected Huh-7 cells were treated with increasing concentrations of REV-ERB agonists for 24 h and viral RNA or NS5A-expressing cells quantified and data expressed relative to Ctrl untreated cells. The experiment was performed in the presence of a neutralising anti-CD81 antibody to limit secondary rounds of infection (mean ± S.E.M., n = 3). e REV-ERB ligands inhibit the replication of diverse HCV genotypes. Huh-7 cells transiently supporting HCV sub-genomic replicons representing genotypes 1–3 were treated with the REV-ERB agonists SR9009 or GSK2667 and replication assessed 24 h later. The dose of agonist required to inhibit HCV RNA replication by 50% (IC 50 ) was determined for all viral genotypes (mean ± S.E.M., n = 3)

    Article Snippet: Purified total RNA samples were tested for HCV RNA expression (Primer Design Ltd, UK) in a qRT-PCR as per the manufacturer’s guidelines (CellsDirect Kit; Invitrogen, UK) in an MxPro-3000 PCR machine (Aligent, US).

    Techniques: Transduction, Expressing, MANN-WHITNEY, Activity Assay, Concentration Assay, Over Expression, Stable Transfection, Transfection, Plasmid Preparation, Western Blot, Infection

    Comparison of the dynamic ranges of the three quantitative HCV RNA assays expressed in HCV international units per milliliter. The dynamic range of each assay is represented by the shaded portion of the corresponding horizontal bar. The 800,000-HCV-IU/ml threshold for tailoring the duration of therapy proposed by Pawlotsky et al. is indicated by the heavy vertical line.

    Journal: Journal of Clinical Microbiology

    Article Title: Comparative Evaluation of the VERSANT HCV RNA 3.0, QUANTIPLEX HCV RNA 2.0, and COBAS AMPLICOR HCV MONITOR Version 2.0 Assays for Quantification of Hepatitis C Virus RNA in Serum

    doi: 10.1128/JCM.40.2.495-500.2002

    Figure Lengend Snippet: Comparison of the dynamic ranges of the three quantitative HCV RNA assays expressed in HCV international units per milliliter. The dynamic range of each assay is represented by the shaded portion of the corresponding horizontal bar. The 800,000-HCV-IU/ml threshold for tailoring the duration of therapy proposed by Pawlotsky et al. is indicated by the heavy vertical line.

    Article Snippet: The relationship between equivalents per milliliter and international units per milliliter was established based on the results from the original collaborative study establishing the WHO international standard for HCV RNA testing ( ) and is currently recommended by the Bayer Corporation.

    Techniques:

    Association between IFN Response, HCV RNA Levels, and Effect on Host Gene Expression in HCV-Infected Mice Intrahepatic HCV RNA levels were determined by quantitative RT-PCR as described in Materials and Methods. Total number of genes showing increased expression in HCV-infected mice relative to donor-matched uninfected mice is shown in gray bars, while the percent of induced genes that are IFN regulated is shown using black triangles.

    Journal: PLoS Pathogens

    Article Title: Host-Specific Response to HCV Infection in the Chimeric SCID-beige/Alb-uPA Mouse Model: Role of the Innate Antiviral Immune Response

    doi: 10.1371/journal.ppat.0020059

    Figure Lengend Snippet: Association between IFN Response, HCV RNA Levels, and Effect on Host Gene Expression in HCV-Infected Mice Intrahepatic HCV RNA levels were determined by quantitative RT-PCR as described in Materials and Methods. Total number of genes showing increased expression in HCV-infected mice relative to donor-matched uninfected mice is shown in gray bars, while the percent of induced genes that are IFN regulated is shown using black triangles.

    Article Snippet: Primer and probe sets for absolute quantification of intrahepatic viral load were designed based on sequences of HCV 1a armored RNA (Ambion Diagnostics, Austin, Texas, United States) using Primer Express (version 3).

    Techniques: Expressing, Infection, Mouse Assay, Quantitative RT-PCR

    Index values of anti-HCV immunoglobulin G in the positive and negative HCV RNA test groups. The index value of anti-HCV was > 10 in 99.1% of those in the positive HCV RNA group but only 19.1% of those in the negative HCV RNA group.

    Journal: Clinical and molecular hepatology

    Article Title: Predicting factors of present hepatitis C virus infection among patients positive for the hepatitis C virus antibody

    doi: 10.3350/cmh.2013.19.4.376

    Figure Lengend Snippet: Index values of anti-HCV immunoglobulin G in the positive and negative HCV RNA test groups. The index value of anti-HCV was > 10 in 99.1% of those in the positive HCV RNA group but only 19.1% of those in the negative HCV RNA group.

    Article Snippet: HCV RNA test HCV RNA was detected by the COBAS TaqMan HCV quantitative assay and the COBAS Amplicor HCV 2.0 qualitative assay (both from Roche Molecular Systems, Branchburg, NJ, USA)., The lower detection limits of the each assay are 15 IU/mL and 50 IU/mL, respectively.

    Techniques:

    NK cells release soluble factor(s) to suppress expression of HCV RNA and NS5A protein in human hepatocytes. (a) Co-culture of NK cells with HCV JFH1-infected Huh7.5.1 cells. At day 4 postinfection, Huh7.5.1 cells in the low compartment of a 24-well transwell co-culture system were co-cultured with primary NK (PNK) or NK92 cells that were added to the top compartment of the co-culture system. (b) Effect of NK SN on HCV JFH1 RNA expression. JFH1-infected Huh7.5.1 cells (day 4 postinfection) were cultured in media conditioned with or without NK SN (PNK or NK92) for 48 h. Total cellular RNA extracted from the hepatic cells was subjected to real-time RT-PCR for HCV and GAPDH RNA quantification. The data are expressed as HCV RNA levels relative to the control (% of control, no NK cells on the top compartment or without treatment with NK SN, which is defined as 100%). The results shown are mean ± SD of triplicate cultures, representative of three separate experiments (*, P

    Journal: Journal of viral hepatitis

    Article Title: Natural killer cells suppress full cycle HCV infection of human hepatocytes

    doi: 10.1111/j.1365-2893.2008.01014.x

    Figure Lengend Snippet: NK cells release soluble factor(s) to suppress expression of HCV RNA and NS5A protein in human hepatocytes. (a) Co-culture of NK cells with HCV JFH1-infected Huh7.5.1 cells. At day 4 postinfection, Huh7.5.1 cells in the low compartment of a 24-well transwell co-culture system were co-cultured with primary NK (PNK) or NK92 cells that were added to the top compartment of the co-culture system. (b) Effect of NK SN on HCV JFH1 RNA expression. JFH1-infected Huh7.5.1 cells (day 4 postinfection) were cultured in media conditioned with or without NK SN (PNK or NK92) for 48 h. Total cellular RNA extracted from the hepatic cells was subjected to real-time RT-PCR for HCV and GAPDH RNA quantification. The data are expressed as HCV RNA levels relative to the control (% of control, no NK cells on the top compartment or without treatment with NK SN, which is defined as 100%). The results shown are mean ± SD of triplicate cultures, representative of three separate experiments (*, P

    Article Snippet: To determine the amount of HCV RNA in culture SN, HCV RNA was extracted from 200 μ L of culture medium by TRI-Reagent-BD (Molecular Research Center, Inc., Cincinnati, OH, USA).

    Techniques: Expressing, Co-Culture Assay, Infection, Cell Culture, RNA Expression, Quantitative RT-PCR

    Time course of inhibition of HCV RNA expression by NK SN. Primary NK (PNK) SN or NK-92 SN was added to HCV JFH1-infected hepatocyte cultures at day 4 postinfection. Intracellular (a) or extracellular (b) RNA was extracted from hepatocytes or culture supernatants (SN) at indicated time points and subjected to the real-time RT-PCR for HCV and GAPDH RNA quantification. Intracellular (a) and extracellular (b) HCV RNA expression is expressed as either HCV RNA 10 7 copies/ μ g cellular RNA or 10 7 copies/mL SN, respectively. The results shown are mean ± SD of triplicate cultures, representative of three experiments (*, P

    Journal: Journal of viral hepatitis

    Article Title: Natural killer cells suppress full cycle HCV infection of human hepatocytes

    doi: 10.1111/j.1365-2893.2008.01014.x

    Figure Lengend Snippet: Time course of inhibition of HCV RNA expression by NK SN. Primary NK (PNK) SN or NK-92 SN was added to HCV JFH1-infected hepatocyte cultures at day 4 postinfection. Intracellular (a) or extracellular (b) RNA was extracted from hepatocytes or culture supernatants (SN) at indicated time points and subjected to the real-time RT-PCR for HCV and GAPDH RNA quantification. Intracellular (a) and extracellular (b) HCV RNA expression is expressed as either HCV RNA 10 7 copies/ μ g cellular RNA or 10 7 copies/mL SN, respectively. The results shown are mean ± SD of triplicate cultures, representative of three experiments (*, P

    Article Snippet: To determine the amount of HCV RNA in culture SN, HCV RNA was extracted from 200 μ L of culture medium by TRI-Reagent-BD (Molecular Research Center, Inc., Cincinnati, OH, USA).

    Techniques: Inhibition, RNA Expression, Infection, Quantitative RT-PCR

    Suppression of HCV RNA expression by NK SN under different conditions. Huh7.5.1 cells were cultured in media conditioned with or without primary NK (PNK) SN or NK92 SN for either 24 h prior to HCV infection, or simultaneously or 8 h postinfection. The cells were then washed five times to remove input HCV after 6 h incubation with HCV JFH1 and then cultured in the presence or absence of NK SN for 8 days. Intracellular (a) and extracellular (b) RNA extracted from hepatocytes or culture supernatants (SN) at day 8 postinfection was subjected to the real-time RT-PCR for HCV and GAPDH RNA quantification. Intracellular (a) and extracellular (b) HCV RNA expression is expressed as HCV RNA levels relative (%) to the control (untreated cell cultures, which are defined as 100%). The results shown are mean ± SD of triplicate cultures, representative of three experiments (*, P

    Journal: Journal of viral hepatitis

    Article Title: Natural killer cells suppress full cycle HCV infection of human hepatocytes

    doi: 10.1111/j.1365-2893.2008.01014.x

    Figure Lengend Snippet: Suppression of HCV RNA expression by NK SN under different conditions. Huh7.5.1 cells were cultured in media conditioned with or without primary NK (PNK) SN or NK92 SN for either 24 h prior to HCV infection, or simultaneously or 8 h postinfection. The cells were then washed five times to remove input HCV after 6 h incubation with HCV JFH1 and then cultured in the presence or absence of NK SN for 8 days. Intracellular (a) and extracellular (b) RNA extracted from hepatocytes or culture supernatants (SN) at day 8 postinfection was subjected to the real-time RT-PCR for HCV and GAPDH RNA quantification. Intracellular (a) and extracellular (b) HCV RNA expression is expressed as HCV RNA levels relative (%) to the control (untreated cell cultures, which are defined as 100%). The results shown are mean ± SD of triplicate cultures, representative of three experiments (*, P

    Article Snippet: To determine the amount of HCV RNA in culture SN, HCV RNA was extracted from 200 μ L of culture medium by TRI-Reagent-BD (Molecular Research Center, Inc., Cincinnati, OH, USA).

    Techniques: RNA Expression, Cell Culture, Infection, Incubation, Quantitative RT-PCR

    IFN- γ is involved in NK SN-mediated anti-HCV activity. (a) IFN- γ production by primary NK (PNK) cells and NK92 cells. IFN- γ levels in media from PNK cells (five donors) and NK-92 cells were determined by ELISA. (b) HCV JFH1-infected Huh7.5.1 cells were cultured in the presence or absence of NK SN and/or antibody (Ab) against IFN- γ . For the cultures using both NK SN and Ab to IFN- γ , the NK SN was preincubated with the Ab to IFN- γ for 30 min before being added to JFH1-infected hepatocyte cultures at day 4 postinfection. IFN- γ (1000 U/mL) alone was added to the cell cultures as a positive control to determine the neutralization ability of the Ab to IFN- γ . Mouse IgG 2A was used to determine the specificity of the Ab to IFN- γ . Total cellular RNA extracted from hepatocytes was subjected to the real-time RT-PCR for HCV and GAPDH RNA quantification at 48 h postexposure to NK SN. The data are expressed as HCV RNA levels relative (% of control, no Abs treatment and no NK SN added, which is defined as 100%). The results shown are mean ± SD of triplicate cultures, representative of three experiments (*, P

    Journal: Journal of viral hepatitis

    Article Title: Natural killer cells suppress full cycle HCV infection of human hepatocytes

    doi: 10.1111/j.1365-2893.2008.01014.x

    Figure Lengend Snippet: IFN- γ is involved in NK SN-mediated anti-HCV activity. (a) IFN- γ production by primary NK (PNK) cells and NK92 cells. IFN- γ levels in media from PNK cells (five donors) and NK-92 cells were determined by ELISA. (b) HCV JFH1-infected Huh7.5.1 cells were cultured in the presence or absence of NK SN and/or antibody (Ab) against IFN- γ . For the cultures using both NK SN and Ab to IFN- γ , the NK SN was preincubated with the Ab to IFN- γ for 30 min before being added to JFH1-infected hepatocyte cultures at day 4 postinfection. IFN- γ (1000 U/mL) alone was added to the cell cultures as a positive control to determine the neutralization ability of the Ab to IFN- γ . Mouse IgG 2A was used to determine the specificity of the Ab to IFN- γ . Total cellular RNA extracted from hepatocytes was subjected to the real-time RT-PCR for HCV and GAPDH RNA quantification at 48 h postexposure to NK SN. The data are expressed as HCV RNA levels relative (% of control, no Abs treatment and no NK SN added, which is defined as 100%). The results shown are mean ± SD of triplicate cultures, representative of three experiments (*, P

    Article Snippet: To determine the amount of HCV RNA in culture SN, HCV RNA was extracted from 200 μ L of culture medium by TRI-Reagent-BD (Molecular Research Center, Inc., Cincinnati, OH, USA).

    Techniques: Activity Assay, Enzyme-linked Immunosorbent Assay, Infection, Cell Culture, Positive Control, Neutralization, Quantitative RT-PCR

    Effect of NK SN on the expression of IFN- α/β in human hepatocytes. (a and c) IFN- α/β RNA expression. At day 4 postinfection, primary NK (PNK) SN or NK-92 SN was added to HCV JFH1-infected Huh7.5.1 cells (25%, v/v). Total cellular RNA was extracted from the cell cultures at 12 h posttreatment with NK SN and then subjected to real-time RT-PCR for IFN- α/β and GAPDH RNA quantification. The data were expressed as IFN- α/β RNA levels relative (fold) to the control (without NK SN, which is defined as 1). The results shown are mean ± SD of triplicate cultures, representative of three separate experiments. (b) IFN- α protein expression. HCV JFH1-infected Huh7.5.1 cells (day 4 postinfection) were cultured in the presence or absence NK (PNK and NK-92) SN (25%, v/v) for 48 h. SNs from the cell cultures were then harvested for IFN- α protein ELISA assay. The results shown are mean ± SD of triplicate cultures (*, P

    Journal: Journal of viral hepatitis

    Article Title: Natural killer cells suppress full cycle HCV infection of human hepatocytes

    doi: 10.1111/j.1365-2893.2008.01014.x

    Figure Lengend Snippet: Effect of NK SN on the expression of IFN- α/β in human hepatocytes. (a and c) IFN- α/β RNA expression. At day 4 postinfection, primary NK (PNK) SN or NK-92 SN was added to HCV JFH1-infected Huh7.5.1 cells (25%, v/v). Total cellular RNA was extracted from the cell cultures at 12 h posttreatment with NK SN and then subjected to real-time RT-PCR for IFN- α/β and GAPDH RNA quantification. The data were expressed as IFN- α/β RNA levels relative (fold) to the control (without NK SN, which is defined as 1). The results shown are mean ± SD of triplicate cultures, representative of three separate experiments. (b) IFN- α protein expression. HCV JFH1-infected Huh7.5.1 cells (day 4 postinfection) were cultured in the presence or absence NK (PNK and NK-92) SN (25%, v/v) for 48 h. SNs from the cell cultures were then harvested for IFN- α protein ELISA assay. The results shown are mean ± SD of triplicate cultures (*, P

    Article Snippet: To determine the amount of HCV RNA in culture SN, HCV RNA was extracted from 200 μ L of culture medium by TRI-Reagent-BD (Molecular Research Center, Inc., Cincinnati, OH, USA).

    Techniques: Expressing, RNA Expression, Infection, Quantitative RT-PCR, Cell Culture, Enzyme-linked Immunosorbent Assay

    Accuracy across the linear range of the CAP/CTM HCV test, v2.0, measured in EDTA plasma (a) and serum (b) matrices. Open diamonds, genotype 1 clinical specimen; black diamonds, armored RNA, genotype 1; light gray diamonds, 3rd WHO HCV RNA international

    Journal: Journal of Clinical Microbiology

    Article Title: Second-Generation Cobas AmpliPrep/Cobas TaqMan HCV Quantitative Test for Viral Load Monitoring: a Novel Dual-Probe Assay Design

    doi: 10.1128/JCM.01784-12

    Figure Lengend Snippet: Accuracy across the linear range of the CAP/CTM HCV test, v2.0, measured in EDTA plasma (a) and serum (b) matrices. Open diamonds, genotype 1 clinical specimen; black diamonds, armored RNA, genotype 1; light gray diamonds, 3rd WHO HCV RNA international

    Article Snippet: A result between the LOD and the LLoQ was reported as > 15 and < 43 IU/ml HCV RNA detected.

    Techniques:

    Deming regression analysis (a) and Bland-Altman analysis (b) of the CAP/CTM HCV test, v2.0, versus the CAP/CTM HCV test for HCV RNA genotypes 1 to 6 and for only genotype 4 in panels c and d. The dotted line (a, c) represents the line of unity, and single

    Journal: Journal of Clinical Microbiology

    Article Title: Second-Generation Cobas AmpliPrep/Cobas TaqMan HCV Quantitative Test for Viral Load Monitoring: a Novel Dual-Probe Assay Design

    doi: 10.1128/JCM.01784-12

    Figure Lengend Snippet: Deming regression analysis (a) and Bland-Altman analysis (b) of the CAP/CTM HCV test, v2.0, versus the CAP/CTM HCV test for HCV RNA genotypes 1 to 6 and for only genotype 4 in panels c and d. The dotted line (a, c) represents the line of unity, and single

    Article Snippet: A result between the LOD and the LLoQ was reported as > 15 and < 43 IU/ml HCV RNA detected.

    Techniques:

    Determination of the linear range of the CAP/CTM HCV test, v2.0, with EDTA plasma (a) and serum (b) matrices. Filled diamonds, clinical samples; open diamonds, armored RNA samples; bold line (from 11 to 1.2E+08 IU/ml), regression line; gray line, line

    Journal: Journal of Clinical Microbiology

    Article Title: Second-Generation Cobas AmpliPrep/Cobas TaqMan HCV Quantitative Test for Viral Load Monitoring: a Novel Dual-Probe Assay Design

    doi: 10.1128/JCM.01784-12

    Figure Lengend Snippet: Determination of the linear range of the CAP/CTM HCV test, v2.0, with EDTA plasma (a) and serum (b) matrices. Filled diamonds, clinical samples; open diamonds, armored RNA samples; bold line (from 11 to 1.2E+08 IU/ml), regression line; gray line, line

    Article Snippet: A result between the LOD and the LLoQ was reported as > 15 and < 43 IU/ml HCV RNA detected.

    Techniques: