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  • 85
    Alomone Labs hcn2
    Hcn2, supplied by Alomone Labs, used in various techniques. Bioz Stars score: 85/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/hcn2/product/Alomone Labs
    Average 85 stars, based on 1 article reviews
    Price from $9.99 to $1999.99
    hcn2 - by Bioz Stars, 2022-11
    85/100 stars
      Buy from Supplier

    94
    Alomone Labs anti hcn2 antibodies
    Effect of over-expression of AC isoforms on <t>HCN2</t> current. A . Original recordings of HCN2. The current was evoked by applying hyperpolarizing voltages from −25 to −85 mV for 5 seconds. B. Average fractional activation of measured HCN2 current. The solid lines are the fits to the Boltzmann function. AC1 activation relation differs from GFP and AC6 (p
    Anti Hcn2 Antibodies, supplied by Alomone Labs, used in various techniques. Bioz Stars score: 94/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/anti hcn2 antibodies/product/Alomone Labs
    Average 94 stars, based on 1 article reviews
    Price from $9.99 to $1999.99
    anti hcn2 antibodies - by Bioz Stars, 2022-11
    94/100 stars
      Buy from Supplier

    hcn 2  (ATCC)
    94
    ATCC hcn 2
    Effect of over-expression of AC isoforms on <t>HCN2</t> current. A . Original recordings of HCN2. The current was evoked by applying hyperpolarizing voltages from −25 to −85 mV for 5 seconds. B. Average fractional activation of measured HCN2 current. The solid lines are the fits to the Boltzmann function. AC1 activation relation differs from GFP and AC6 (p
    Hcn 2, supplied by ATCC, used in various techniques. Bioz Stars score: 94/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/hcn 2/product/ATCC
    Average 94 stars, based on 1 article reviews
    Price from $9.99 to $1999.99
    hcn 2 - by Bioz Stars, 2022-11
    94/100 stars
      Buy from Supplier

    Image Search Results


    Effect of over-expression of AC isoforms on HCN2 current. A . Original recordings of HCN2. The current was evoked by applying hyperpolarizing voltages from −25 to −85 mV for 5 seconds. B. Average fractional activation of measured HCN2 current. The solid lines are the fits to the Boltzmann function. AC1 activation relation differs from GFP and AC6 (p

    Journal: Journal of molecular and cellular cardiology

    Article Title: CA2+-ACTIVATED ADENYLYL CYCLASE 1 INTRODUCES CA2+-DEPENDENCE TO BETA-ADRENERGIC STIMULATION OF HCN2 CURRENT

    doi: 10.1016/j.yjmcc.2012.03.010

    Figure Lengend Snippet: Effect of over-expression of AC isoforms on HCN2 current. A . Original recordings of HCN2. The current was evoked by applying hyperpolarizing voltages from −25 to −85 mV for 5 seconds. B. Average fractional activation of measured HCN2 current. The solid lines are the fits to the Boltzmann function. AC1 activation relation differs from GFP and AC6 (p

    Article Snippet: HCN2 was pulled down with 2μg of anti-HCN2 antibodies (APC-030; Alomone Labs, Israel) per 500 μg of soluble materials.

    Techniques: Over Expression, Activation Assay

    A. Expression of recombinant adenylyl cyclase in NRVM. Cultures were co-infected with AdmHCN2 and one of the following: AdGFP, FLAG-tagged AdAC1 or FLAG-tagged AdAC6. 72 hours later cells were harvested, and the soluble membrane fraction was isolated. Soluble membranes from cultures infected with AdGFP, AdAC1 or AdAC6 were incubated with anti-FLAG or anti-HCN2 antibodies, separated by PAGE and probed with appropriate antibodies. B . Expression of endogenous HCN2 in NRVM and its co-immunoprecipitation with overexpressed adenylyl cyclases. The top panel shows detection of HCN2 in membrane fraction and the bottom panel – in anti-FLAG-immunoprecipitates.

    Journal: Journal of molecular and cellular cardiology

    Article Title: CA2+-ACTIVATED ADENYLYL CYCLASE 1 INTRODUCES CA2+-DEPENDENCE TO BETA-ADRENERGIC STIMULATION OF HCN2 CURRENT

    doi: 10.1016/j.yjmcc.2012.03.010

    Figure Lengend Snippet: A. Expression of recombinant adenylyl cyclase in NRVM. Cultures were co-infected with AdmHCN2 and one of the following: AdGFP, FLAG-tagged AdAC1 or FLAG-tagged AdAC6. 72 hours later cells were harvested, and the soluble membrane fraction was isolated. Soluble membranes from cultures infected with AdGFP, AdAC1 or AdAC6 were incubated with anti-FLAG or anti-HCN2 antibodies, separated by PAGE and probed with appropriate antibodies. B . Expression of endogenous HCN2 in NRVM and its co-immunoprecipitation with overexpressed adenylyl cyclases. The top panel shows detection of HCN2 in membrane fraction and the bottom panel – in anti-FLAG-immunoprecipitates.

    Article Snippet: HCN2 was pulled down with 2μg of anti-HCN2 antibodies (APC-030; Alomone Labs, Israel) per 500 μg of soluble materials.

    Techniques: Expressing, Recombinant, Infection, Isolation, Incubation, Polyacrylamide Gel Electrophoresis, Immunoprecipitation

    Increased expression of hyperpolarization-activated cyclic nucleotide-gated 1 (HCN1) and HCN2 in spiral ganglion neurons (SGNs) of old mice. (A) Auditory brainstem response (ABR) hearing thresholds were measured from young and old mice ( n = 8). Arrows indicate that thresholds exceeded the upper limits of the tucker davis technologies (TDT) ABR system. (B) Real-time PCR of HCN subunits in SGNs from young and old mice, showed a significant increase of HCN1 and HCN2 at the mRNA level in old mice ( n = 6). (C) Western blotting showed HCN1 and HCN2 expression level was significantly increased in the SGNs of old mice, but HCN3 and HCN4 were unchanged ( n = 4). Data are means ± SEM, * p

    Journal: Frontiers in Aging Neuroscience

    Article Title: Age-Dependent Up-Regulation of HCN Channels in Spiral Ganglion Neurons Coincide With Hearing Loss in Mice

    doi: 10.3389/fnagi.2018.00353

    Figure Lengend Snippet: Increased expression of hyperpolarization-activated cyclic nucleotide-gated 1 (HCN1) and HCN2 in spiral ganglion neurons (SGNs) of old mice. (A) Auditory brainstem response (ABR) hearing thresholds were measured from young and old mice ( n = 8). Arrows indicate that thresholds exceeded the upper limits of the tucker davis technologies (TDT) ABR system. (B) Real-time PCR of HCN subunits in SGNs from young and old mice, showed a significant increase of HCN1 and HCN2 at the mRNA level in old mice ( n = 6). (C) Western blotting showed HCN1 and HCN2 expression level was significantly increased in the SGNs of old mice, but HCN3 and HCN4 were unchanged ( n = 4). Data are means ± SEM, * p

    Article Snippet: The following primary antibodies were used: rabbit anti-HCN1, anti-HCN2, anti-HCN3, anti-HCN4 (Alomone Labs), mouse anti-AIF (Gene Tex), rabbit anti cleaved caspase-3 (Cell Signaling Technology) and mouse anti neuronal class III ß-Tubulin (TUJ1; Covance).

    Techniques: Expressing, Mouse Assay, Real-time Polymerase Chain Reaction, Western Blot