Journal: Frontiers in Aging Neuroscience
Article Title: Age-Dependent Up-Regulation of HCN Channels in Spiral Ganglion Neurons Coincide With Hearing Loss in Mice
Figure Lengend Snippet: Increased expression of hyperpolarization-activated cyclic nucleotide-gated 1 (HCN1) and HCN2 in spiral ganglion neurons (SGNs) of old mice. (A) Auditory brainstem response (ABR) hearing thresholds were measured from young and old mice ( n = 8). Arrows indicate that thresholds exceeded the upper limits of the tucker davis technologies (TDT) ABR system. (B) Real-time PCR of HCN subunits in SGNs from young and old mice, showed a significant increase of HCN1 and HCN2 at the mRNA level in old mice ( n = 6). (C) Western blotting showed HCN1 and HCN2 expression level was significantly increased in the SGNs of old mice, but HCN3 and HCN4 were unchanged ( n = 4). Data are means ± SEM, * p
Article Snippet: The following primary antibodies were used: rabbit anti-HCN1, anti-HCN2, anti-HCN3, anti-HCN4 (Alomone Labs), mouse anti-AIF (Gene Tex), rabbit anti cleaved caspase-3 (Cell Signaling Technology) and mouse anti neuronal class III ß-Tubulin (TUJ1; Covance).
Techniques: Expressing, Mouse Assay, Real-time Polymerase Chain Reaction, Western Blot