hcmv ad169 strain Search Results


hcmv strain ad169  (ATCC)
99
ATCC hcmv strain ad169
Construction of I-site mutant HCMV. (A) HCMV proteinase precursor, its four cleavage sites, and their P3 to P1′ cleavage sequences (1, 55). (B) Amino acid and corresponding nucleotide sequences of the native (left) and mutant (right) I sites, with the new NruI restriction enzyme site underlined. The Ala codon, GCC, was mutated to the Val codon, GTC, by a C-to-T change at nucleotide 115625 (italicized) in the <t>AD169</t> sequence (6). (C) Homologous recombination between shuttle plasmid EB39 and HCMV-BAC, pHB5, that transfers the A143V-coding mutation into the viral genome to form mutant HCMV-BAC, CKC1. The asterisk denotes a mutation.
Hcmv Strain Ad169, supplied by ATCC, used in various techniques. Bioz Stars score: 99/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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hcmv strain ad 169  (Virusys Inc)
86
Virusys Inc hcmv strain ad 169
Construction of I-site mutant HCMV. (A) HCMV proteinase precursor, its four cleavage sites, and their P3 to P1′ cleavage sequences (1, 55). (B) Amino acid and corresponding nucleotide sequences of the native (left) and mutant (right) I sites, with the new NruI restriction enzyme site underlined. The Ala codon, GCC, was mutated to the Val codon, GTC, by a C-to-T change at nucleotide 115625 (italicized) in the <t>AD169</t> sequence (6). (C) Homologous recombination between shuttle plasmid EB39 and HCMV-BAC, pHB5, that transfers the A143V-coding mutation into the viral genome to form mutant HCMV-BAC, CKC1. The asterisk denotes a mutation.
Hcmv Strain Ad 169, supplied by Virusys Inc, used in various techniques. Bioz Stars score: 86/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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hcmv strain ad 169 - by Bioz Stars, 2024-12
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hcmv strain ad169  (Becton Dickinson)
86
Becton Dickinson hcmv strain ad169
Construction of I-site mutant HCMV. (A) HCMV proteinase precursor, its four cleavage sites, and their P3 to P1′ cleavage sequences (1, 55). (B) Amino acid and corresponding nucleotide sequences of the native (left) and mutant (right) I sites, with the new NruI restriction enzyme site underlined. The Ala codon, GCC, was mutated to the Val codon, GTC, by a C-to-T change at nucleotide 115625 (italicized) in the <t>AD169</t> sequence (6). (C) Homologous recombination between shuttle plasmid EB39 and HCMV-BAC, pHB5, that transfers the A143V-coding mutation into the viral genome to form mutant HCMV-BAC, CKC1. The asterisk denotes a mutation.
Hcmv Strain Ad169, supplied by Becton Dickinson, used in various techniques. Bioz Stars score: 86/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/result/hcmv strain ad169/product/Becton Dickinson
Average 86 stars, based on 1 article reviews
Price from $9.99 to $1999.99
hcmv strain ad169 - by Bioz Stars, 2024-12
86/100 stars
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hcmv strain ad169  (Thermo Fisher)
86
Thermo Fisher hcmv strain ad169
Construction of I-site mutant HCMV. (A) HCMV proteinase precursor, its four cleavage sites, and their P3 to P1′ cleavage sequences (1, 55). (B) Amino acid and corresponding nucleotide sequences of the native (left) and mutant (right) I sites, with the new NruI restriction enzyme site underlined. The Ala codon, GCC, was mutated to the Val codon, GTC, by a C-to-T change at nucleotide 115625 (italicized) in the <t>AD169</t> sequence (6). (C) Homologous recombination between shuttle plasmid EB39 and HCMV-BAC, pHB5, that transfers the A143V-coding mutation into the viral genome to form mutant HCMV-BAC, CKC1. The asterisk denotes a mutation.
Hcmv Strain Ad169, supplied by Thermo Fisher, used in various techniques. Bioz Stars score: 86/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/result/hcmv strain ad169/product/Thermo Fisher
Average 86 stars, based on 1 article reviews
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ad169 strain hcmv  (Millipore)
86
Millipore ad169 strain hcmv
KEY RESOURCES TABLE
Ad169 Strain Hcmv, supplied by Millipore, used in various techniques. Bioz Stars score: 86/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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Image Search Results


Construction of I-site mutant HCMV. (A) HCMV proteinase precursor, its four cleavage sites, and their P3 to P1′ cleavage sequences (1, 55). (B) Amino acid and corresponding nucleotide sequences of the native (left) and mutant (right) I sites, with the new NruI restriction enzyme site underlined. The Ala codon, GCC, was mutated to the Val codon, GTC, by a C-to-T change at nucleotide 115625 (italicized) in the AD169 sequence (6). (C) Homologous recombination between shuttle plasmid EB39 and HCMV-BAC, pHB5, that transfers the A143V-coding mutation into the viral genome to form mutant HCMV-BAC, CKC1. The asterisk denotes a mutation.

Journal:

Article Title: Cytomegalovirus Assemblin (pUL80a): Cleavage at Internal Site Not Essential for Virus Growth; Proteinase Absent from Virions

doi: 10.1128/JVI.76.17.8667-8674.2002

Figure Lengend Snippet: Construction of I-site mutant HCMV. (A) HCMV proteinase precursor, its four cleavage sites, and their P3 to P1′ cleavage sequences (1, 55). (B) Amino acid and corresponding nucleotide sequences of the native (left) and mutant (right) I sites, with the new NruI restriction enzyme site underlined. The Ala codon, GCC, was mutated to the Val codon, GTC, by a C-to-T change at nucleotide 115625 (italicized) in the AD169 sequence (6). (C) Homologous recombination between shuttle plasmid EB39 and HCMV-BAC, pHB5, that transfers the A143V-coding mutation into the viral genome to form mutant HCMV-BAC, CKC1. The asterisk denotes a mutation.

Article Snippet: HCMV strain AD169 was from the American Type Culture Collection (no. VR-538; Manassas, Va.).

Techniques: Mutagenesis, Sequencing, Homologous Recombination, Plasmid Preparation

DNA from I-site mutant virus contains mutagenic nucleotide change. A 1,257-bp sequence beginning at the 5′ end of UL80 was amplified by PCR from five DNA templates and analyzed following incubation without (No Enzyme) or with (+NruI) NruI. Shown is an image prepared from a 1% agarose gel containing the resulting DNA fragments stained with ethidium bromide, together with molecular weight markers (MW, lane 1). PCR templates were wild-type (Wt) and mutant (I−) HCMV-BACs (pBAC; lanes 2 and 7 and lanes 3 and 8, respectively), mutant and wild-type HCMV-BAC virions (BACvir; lanes 4 and 9 and lanes 5 and 10, respectively), and standard AD169 virions (Vir, lanes 6 and 11). Fragment sizes (base pairs) are indicated at the right.

Journal:

Article Title: Cytomegalovirus Assemblin (pUL80a): Cleavage at Internal Site Not Essential for Virus Growth; Proteinase Absent from Virions

doi: 10.1128/JVI.76.17.8667-8674.2002

Figure Lengend Snippet: DNA from I-site mutant virus contains mutagenic nucleotide change. A 1,257-bp sequence beginning at the 5′ end of UL80 was amplified by PCR from five DNA templates and analyzed following incubation without (No Enzyme) or with (+NruI) NruI. Shown is an image prepared from a 1% agarose gel containing the resulting DNA fragments stained with ethidium bromide, together with molecular weight markers (MW, lane 1). PCR templates were wild-type (Wt) and mutant (I−) HCMV-BACs (pBAC; lanes 2 and 7 and lanes 3 and 8, respectively), mutant and wild-type HCMV-BAC virions (BACvir; lanes 4 and 9 and lanes 5 and 10, respectively), and standard AD169 virions (Vir, lanes 6 and 11). Fragment sizes (base pairs) are indicated at the right.

Article Snippet: HCMV strain AD169 was from the American Type Culture Collection (no. VR-538; Manassas, Va.).

Techniques: Mutagenesis, Sequencing, Amplification, Incubation, Agarose Gel Electrophoresis, Staining, Molecular Weight

KEY RESOURCES TABLE

Journal: Cell reports

Article Title: SAMHD1 Modulates Early Steps during Human Cytomegalovirus Infection by Limiting NF-κB Activation

doi: 10.1016/j.celrep.2019.06.027

Figure Lengend Snippet: KEY RESOURCES TABLE

Article Snippet: All the cells have been tested to be mycoplasma-free by the LookOut Mycoplasma PCR Detection Kit (Sigma-Aldrich) Viruses TB40/E strain HCMV expressing GFP, TB40/E strain HCMV expressing mCherry and an IE2-GFP or UL99-GFP fusion, and an AD169 strain HCMV as wild-type and K355Q mutant virus were propagated in HFF cells.

Techniques: Recombinant, Silver Staining, Transfection, SYBR Green Assay, Negative Control, Software, Imaging, Real-time Polymerase Chain Reaction