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  • 99
    Worthington Biochemical hbss
    Hbss, supplied by Worthington Biochemical, used in various techniques. Bioz Stars score: 99/100, based on 1360 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/hbss/product/Worthington Biochemical
    Average 99 stars, based on 1360 article reviews
    Price from $9.99 to $1999.99
    hbss - by Bioz Stars, 2020-12
    99/100 stars
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    99
    Thermo Fisher hbss
    Hbss, supplied by Thermo Fisher, used in various techniques. Bioz Stars score: 99/100, based on 28272 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/hbss/product/Thermo Fisher
    Average 99 stars, based on 28272 article reviews
    Price from $9.99 to $1999.99
    hbss - by Bioz Stars, 2020-12
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    99
    Thermo Fisher hank s balanced salt solution hbss
    Hank S Balanced Salt Solution Hbss, supplied by Thermo Fisher, used in various techniques. Bioz Stars score: 99/100, based on 3339 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/hank s balanced salt solution hbss/product/Thermo Fisher
    Average 99 stars, based on 3339 article reviews
    Price from $9.99 to $1999.99
    hank s balanced salt solution hbss - by Bioz Stars, 2020-12
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    99
    Millipore balanced salt solution hbss
    Balanced Salt Solution Hbss, supplied by Millipore, used in various techniques. Bioz Stars score: 99/100, based on 1239 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/balanced salt solution hbss/product/Millipore
    Average 99 stars, based on 1239 article reviews
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    balanced salt solution hbss - by Bioz Stars, 2020-12
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    99
    Thermo Fisher hbss calcium
    Hbss Calcium, supplied by Thermo Fisher, used in various techniques. Bioz Stars score: 99/100, based on 1145 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/hbss calcium/product/Thermo Fisher
    Average 99 stars, based on 1145 article reviews
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    hbss calcium - by Bioz Stars, 2020-12
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    99
    Thermo Fisher magnesium
    Magnesium, supplied by Thermo Fisher, used in various techniques. Bioz Stars score: 99/100, based on 2458 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/magnesium/product/Thermo Fisher
    Average 99 stars, based on 2458 article reviews
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    magnesium - by Bioz Stars, 2020-12
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    93
    Mediatech hbss
    Amyloidogenic V122I but not non-amyloidogenic T119M <t>TTR</t> induces the generation of superoxide species and caspase 3/7 activation in human cardiomyocytes ( a ) AC16 cells were treated with V122I TTR, T119M TTR, Antimycin A (positive control) or <t>HBSS</t> (vehicle control) for 24 h. The insults were then removed and the cells loaded with the superoxide-specific reagent DHE as detailed in the experimental section. Red fluorescence produced by DHE oxidation is shown. ( b ) AC16 cells were treated with V122I TTR, T119M TTR or, HBSS for 6 h at 37°C. Cell lysates were prepared as detailed in the experimental section and were added to Ac-DEVD-AFC caspase 3/7 substrate. Fluorescence intensity generated by cleavage of the substrate was measured. Asterisks in both panels indicate statistical significance with P
    Hbss, supplied by Mediatech, used in various techniques. Bioz Stars score: 93/100, based on 816 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/hbss/product/Mediatech
    Average 93 stars, based on 816 article reviews
    Price from $9.99 to $1999.99
    hbss - by Bioz Stars, 2020-12
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    92
    Cellgro hbss
    Amyloidogenic V122I but not non-amyloidogenic T119M <t>TTR</t> induces the generation of superoxide species and caspase 3/7 activation in human cardiomyocytes ( a ) AC16 cells were treated with V122I TTR, T119M TTR, Antimycin A (positive control) or <t>HBSS</t> (vehicle control) for 24 h. The insults were then removed and the cells loaded with the superoxide-specific reagent DHE as detailed in the experimental section. Red fluorescence produced by DHE oxidation is shown. ( b ) AC16 cells were treated with V122I TTR, T119M TTR or, HBSS for 6 h at 37°C. Cell lysates were prepared as detailed in the experimental section and were added to Ac-DEVD-AFC caspase 3/7 substrate. Fluorescence intensity generated by cleavage of the substrate was measured. Asterisks in both panels indicate statistical significance with P
    Hbss, supplied by Cellgro, used in various techniques. Bioz Stars score: 92/100, based on 580 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/hbss/product/Cellgro
    Average 92 stars, based on 580 article reviews
    Price from $9.99 to $1999.99
    hbss - by Bioz Stars, 2020-12
    92/100 stars
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    93
    Corning Life Sciences hbss
    Amyloidogenic V122I but not non-amyloidogenic T119M <t>TTR</t> induces the generation of superoxide species and caspase 3/7 activation in human cardiomyocytes ( a ) AC16 cells were treated with V122I TTR, T119M TTR, Antimycin A (positive control) or <t>HBSS</t> (vehicle control) for 24 h. The insults were then removed and the cells loaded with the superoxide-specific reagent DHE as detailed in the experimental section. Red fluorescence produced by DHE oxidation is shown. ( b ) AC16 cells were treated with V122I TTR, T119M TTR or, HBSS for 6 h at 37°C. Cell lysates were prepared as detailed in the experimental section and were added to Ac-DEVD-AFC caspase 3/7 substrate. Fluorescence intensity generated by cleavage of the substrate was measured. Asterisks in both panels indicate statistical significance with P
    Hbss, supplied by Corning Life Sciences, used in various techniques. Bioz Stars score: 93/100, based on 824 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/hbss/product/Corning Life Sciences
    Average 93 stars, based on 824 article reviews
    Price from $9.99 to $1999.99
    hbss - by Bioz Stars, 2020-12
    93/100 stars
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    N/A
    HBSS 10X is a concentrated formula of Hank s Balanced Salt Solution HBSS 10X can be used in an assortment of cell culture applications such as diluting cells for counting
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    Image Search Results


    Amyloidogenic V122I but not non-amyloidogenic T119M TTR induces the generation of superoxide species and caspase 3/7 activation in human cardiomyocytes ( a ) AC16 cells were treated with V122I TTR, T119M TTR, Antimycin A (positive control) or HBSS (vehicle control) for 24 h. The insults were then removed and the cells loaded with the superoxide-specific reagent DHE as detailed in the experimental section. Red fluorescence produced by DHE oxidation is shown. ( b ) AC16 cells were treated with V122I TTR, T119M TTR or, HBSS for 6 h at 37°C. Cell lysates were prepared as detailed in the experimental section and were added to Ac-DEVD-AFC caspase 3/7 substrate. Fluorescence intensity generated by cleavage of the substrate was measured. Asterisks in both panels indicate statistical significance with P

    Journal: Bioscience Reports

    Article Title: Amyloidogenic and non-amyloidogenic transthyretin variants interact differently with human cardiomyocytes: insights into early events of non-fibrillar tissue damage

    doi: 10.1042/BSR20140155

    Figure Lengend Snippet: Amyloidogenic V122I but not non-amyloidogenic T119M TTR induces the generation of superoxide species and caspase 3/7 activation in human cardiomyocytes ( a ) AC16 cells were treated with V122I TTR, T119M TTR, Antimycin A (positive control) or HBSS (vehicle control) for 24 h. The insults were then removed and the cells loaded with the superoxide-specific reagent DHE as detailed in the experimental section. Red fluorescence produced by DHE oxidation is shown. ( b ) AC16 cells were treated with V122I TTR, T119M TTR or, HBSS for 6 h at 37°C. Cell lysates were prepared as detailed in the experimental section and were added to Ac-DEVD-AFC caspase 3/7 substrate. Fluorescence intensity generated by cleavage of the substrate was measured. Asterisks in both panels indicate statistical significance with P

    Article Snippet: When the recombinant TTR was intended to be used for biophysical studies, the gel filtration purification was performed in 10 mM phosphate buffer (sodium) pH 7.6/100 mM KCl/1 mM EDTA buffer (GF buffer); when the TTR was intended for cell culture experiments, HBSS (Hank's balanced salt solution; Mediatech) buffer was used instead.

    Techniques: Activation Assay, Positive Control, Fluorescence, Produced, Generated

    Native V122I TTR competes with f-V122I TTR for the same interaction sites in human cardiomyocytes ( a ) AC16 cells were co-incubated with f-V122I TTR (320 nM) and increasing concentrations of amyloidogenic V122I TTR or non-amyloidogenic T119M TTR for 3 h at 4°C. The fluorescence associated with the cells was measured and normalized for total protein content. The data were normalized by the fluorescence from the samples incubated with f-V122I TTR only (100%). Filled triangles, V122I TTR; open circles, T119M TTR. ( b ) AC16 cells were co-incubated with f-V122I (600 nM) and equimolar amounts of unlabelled V122I TTR, T119M TTR, V122I stabilized with a small molecule (V122I-SM) or HBSS. Fluorescence associated with the cells was measured, normalized by total protein content and plotted as percentage with respect to the fluorescence of cells that had been incubated with f-V122I and HBSS only. The data show that similarly to T119M TTR, V122I-SM is unable to efficiently displace cell-associated f-V122I, whereas under the same conditions V122I TTR displaces more than 50% of f-V122I TTR.

    Journal: Bioscience Reports

    Article Title: Amyloidogenic and non-amyloidogenic transthyretin variants interact differently with human cardiomyocytes: insights into early events of non-fibrillar tissue damage

    doi: 10.1042/BSR20140155

    Figure Lengend Snippet: Native V122I TTR competes with f-V122I TTR for the same interaction sites in human cardiomyocytes ( a ) AC16 cells were co-incubated with f-V122I TTR (320 nM) and increasing concentrations of amyloidogenic V122I TTR or non-amyloidogenic T119M TTR for 3 h at 4°C. The fluorescence associated with the cells was measured and normalized for total protein content. The data were normalized by the fluorescence from the samples incubated with f-V122I TTR only (100%). Filled triangles, V122I TTR; open circles, T119M TTR. ( b ) AC16 cells were co-incubated with f-V122I (600 nM) and equimolar amounts of unlabelled V122I TTR, T119M TTR, V122I stabilized with a small molecule (V122I-SM) or HBSS. Fluorescence associated with the cells was measured, normalized by total protein content and plotted as percentage with respect to the fluorescence of cells that had been incubated with f-V122I and HBSS only. The data show that similarly to T119M TTR, V122I-SM is unable to efficiently displace cell-associated f-V122I, whereas under the same conditions V122I TTR displaces more than 50% of f-V122I TTR.

    Article Snippet: When the recombinant TTR was intended to be used for biophysical studies, the gel filtration purification was performed in 10 mM phosphate buffer (sodium) pH 7.6/100 mM KCl/1 mM EDTA buffer (GF buffer); when the TTR was intended for cell culture experiments, HBSS (Hank's balanced salt solution; Mediatech) buffer was used instead.

    Techniques: Incubation, Fluorescence

    Differential interaction of the amyloidogenic V122I TTR and the non-amyloidogenic T119M TTR with the human cardiac cell line AC16 AC16 cells were treated with trypsin (or HBSS as control) to remove membrane-associated proteins. V122I and T119M TTR were then incubated with the cells for 4 h at 4°C or 37°C. Cell lysates were prepared and analysed by SDS—PAGE (15% gel) and Western blot using an anti-TTR and anti-actin antibodies. The lower panel shows densitometry analysis (absorbance) of total cell-associated TTR normalized to actin. Error bars represent S.D. of the biological duplicates. The percentage of TTR/actin ratio in cells pre-treated with trypsin (trypsin +) with respect to control cells (trypsin −) is shown.

    Journal: Bioscience Reports

    Article Title: Amyloidogenic and non-amyloidogenic transthyretin variants interact differently with human cardiomyocytes: insights into early events of non-fibrillar tissue damage

    doi: 10.1042/BSR20140155

    Figure Lengend Snippet: Differential interaction of the amyloidogenic V122I TTR and the non-amyloidogenic T119M TTR with the human cardiac cell line AC16 AC16 cells were treated with trypsin (or HBSS as control) to remove membrane-associated proteins. V122I and T119M TTR were then incubated with the cells for 4 h at 4°C or 37°C. Cell lysates were prepared and analysed by SDS—PAGE (15% gel) and Western blot using an anti-TTR and anti-actin antibodies. The lower panel shows densitometry analysis (absorbance) of total cell-associated TTR normalized to actin. Error bars represent S.D. of the biological duplicates. The percentage of TTR/actin ratio in cells pre-treated with trypsin (trypsin +) with respect to control cells (trypsin −) is shown.

    Article Snippet: When the recombinant TTR was intended to be used for biophysical studies, the gel filtration purification was performed in 10 mM phosphate buffer (sodium) pH 7.6/100 mM KCl/1 mM EDTA buffer (GF buffer); when the TTR was intended for cell culture experiments, HBSS (Hank's balanced salt solution; Mediatech) buffer was used instead.

    Techniques: Incubation, SDS Page, Western Blot