hantavirus pool 1 -- Search Results


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  • 99
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    Orthohantaviruses hinder staurosporine-induced apoptosis in human primary endothelial cells. ( a ) Representative immunofluorescence images from three independent experiments of ANDV-, HTNV-, SEOV-, PUUV-, PHV- and TULV-infected HUVEC after exposure to staurosporine (2 μM) for approximately 4 hours. Cells were infected at a low MOI (0.01) to achieve 20 to 30% infection at 4 days post-infection. Following staurosporine-treatment, cells were stained with TUNEL (red) to assess apoptosis, convalescent PUUV patient serum (green) to detect virus infection and DAPI (blue) for nuclear counterstaining. Scale bar, 20 μm. ( b ) Graphs showing TUNEL-positive uninfected and infected cells. Data shown represent the mean ± SD of three independent experiments. ( c ). Specific monoclonal antibodies (mabs 1C12 for ANDV, HTNV, SEOV, PUUV and TULV, and 7B3F7 for PHV) were used for detection of N protein. <t>Calnexin</t> was used as loading control. One representative experiment out of three independent experiments is shown. Band intensity was analysed by densitometry of caspase-3 cleaved PARP and full-length PARP. The ratios in intensity between cleaved and full-length PARP were calculated and compared between infected and uninfected conditions. The ratio from uninfected cells represents maximal caspase-3 cleaved PARP/full-length PARP ratio (100%). The graphs show mean ± SD of the ratio between cleaved and full-length PARP from three experiments. Paired t-test: *p
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    Orthohantaviruses hinder staurosporine-induced apoptosis in human primary endothelial cells. ( a ) Representative immunofluorescence images from three independent experiments of ANDV-, HTNV-, SEOV-, PUUV-, PHV- and TULV-infected HUVEC after exposure to staurosporine (2 μM) for approximately 4 hours. Cells were infected at a low MOI (0.01) to achieve 20 to 30% infection at 4 days post-infection. Following staurosporine-treatment, cells were stained with TUNEL (red) to assess apoptosis, convalescent PUUV patient serum (green) to detect virus infection and DAPI (blue) for nuclear counterstaining. Scale bar, 20 μm. ( b ) Graphs showing TUNEL-positive uninfected and infected cells. Data shown represent the mean ± SD of three independent experiments. ( c ). Specific monoclonal antibodies (mabs 1C12 for ANDV, HTNV, SEOV, PUUV and TULV, and 7B3F7 for PHV) were used for detection of N protein. <t>Calnexin</t> was used as loading control. One representative experiment out of three independent experiments is shown. Band intensity was analysed by densitometry of caspase-3 cleaved PARP and full-length PARP. The ratios in intensity between cleaved and full-length PARP were calculated and compared between infected and uninfected conditions. The ratio from uninfected cells represents maximal caspase-3 cleaved PARP/full-length PARP ratio (100%). The graphs show mean ± SD of the ratio between cleaved and full-length PARP from three experiments. Paired t-test: *p
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    Orthohantaviruses hinder staurosporine-induced apoptosis in human primary endothelial cells. ( a ) Representative immunofluorescence images from three independent experiments of ANDV-, HTNV-, SEOV-, PUUV-, PHV- and TULV-infected HUVEC after exposure to staurosporine (2 μM) for approximately 4 hours. Cells were infected at a low MOI (0.01) to achieve 20 to 30% infection at 4 days post-infection. Following staurosporine-treatment, cells were stained with TUNEL (red) to assess apoptosis, convalescent PUUV patient serum (green) to detect virus infection and DAPI (blue) for nuclear counterstaining. Scale bar, 20 μm. ( b ) Graphs showing TUNEL-positive uninfected and infected cells. Data shown represent the mean ± SD of three independent experiments. ( c ). Specific monoclonal antibodies (mabs 1C12 for ANDV, HTNV, SEOV, PUUV and TULV, and 7B3F7 for PHV) were used for detection of N protein. <t>Calnexin</t> was used as loading control. One representative experiment out of three independent experiments is shown. Band intensity was analysed by densitometry of caspase-3 cleaved PARP and full-length PARP. The ratios in intensity between cleaved and full-length PARP were calculated and compared between infected and uninfected conditions. The ratio from uninfected cells represents maximal caspase-3 cleaved PARP/full-length PARP ratio (100%). The graphs show mean ± SD of the ratio between cleaved and full-length PARP from three experiments. Paired t-test: *p
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    Orthohantaviruses hinder staurosporine-induced apoptosis in human primary endothelial cells. ( a ) Representative immunofluorescence images from three independent experiments of ANDV-, HTNV-, SEOV-, PUUV-, PHV- and TULV-infected HUVEC after exposure to staurosporine (2 μM) for approximately 4 hours. Cells were infected at a low MOI (0.01) to achieve 20 to 30% infection at 4 days post-infection. Following staurosporine-treatment, cells were stained with TUNEL (red) to assess apoptosis, convalescent PUUV patient serum (green) to detect virus infection and DAPI (blue) for nuclear counterstaining. Scale bar, 20 μm. ( b ) Graphs showing TUNEL-positive uninfected and infected cells. Data shown represent the mean ± SD of three independent experiments. ( c ). Specific monoclonal antibodies (mabs 1C12 for ANDV, HTNV, SEOV, PUUV and TULV, and 7B3F7 for PHV) were used for detection of N protein. <t>Calnexin</t> was used as loading control. One representative experiment out of three independent experiments is shown. Band intensity was analysed by densitometry of caspase-3 cleaved PARP and full-length PARP. The ratios in intensity between cleaved and full-length PARP were calculated and compared between infected and uninfected conditions. The ratio from uninfected cells represents maximal caspase-3 cleaved PARP/full-length PARP ratio (100%). The graphs show mean ± SD of the ratio between cleaved and full-length PARP from three experiments. Paired t-test: *p
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    Orthohantaviruses hinder staurosporine-induced apoptosis in human primary endothelial cells. ( a ) Representative immunofluorescence images from three independent experiments of ANDV-, HTNV-, SEOV-, PUUV-, PHV- and TULV-infected HUVEC after exposure to staurosporine (2 μM) for approximately 4 hours. Cells were infected at a low MOI (0.01) to achieve 20 to 30% infection at 4 days post-infection. Following staurosporine-treatment, cells were stained with TUNEL (red) to assess apoptosis, convalescent PUUV patient serum (green) to detect virus infection and DAPI (blue) for nuclear counterstaining. Scale bar, 20 μm. ( b ) Graphs showing TUNEL-positive uninfected and infected cells. Data shown represent the mean ± SD of three independent experiments. ( c ). Specific monoclonal antibodies (mabs 1C12 for ANDV, HTNV, SEOV, PUUV and TULV, and 7B3F7 for PHV) were used for detection of N protein. <t>Calnexin</t> was used as loading control. One representative experiment out of three independent experiments is shown. Band intensity was analysed by densitometry of caspase-3 cleaved PARP and full-length PARP. The ratios in intensity between cleaved and full-length PARP were calculated and compared between infected and uninfected conditions. The ratio from uninfected cells represents maximal caspase-3 cleaved PARP/full-length PARP ratio (100%). The graphs show mean ± SD of the ratio between cleaved and full-length PARP from three experiments. Paired t-test: *p
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    Orthohantaviruses hinder staurosporine-induced apoptosis in human primary endothelial cells. ( a ) Representative immunofluorescence images from three independent experiments of ANDV-, HTNV-, SEOV-, PUUV-, PHV- and TULV-infected HUVEC after exposure to staurosporine (2 μM) for approximately 4 hours. Cells were infected at a low MOI (0.01) to achieve 20 to 30% infection at 4 days post-infection. Following staurosporine-treatment, cells were stained with TUNEL (red) to assess apoptosis, convalescent PUUV patient serum (green) to detect virus infection and DAPI (blue) for nuclear counterstaining. Scale bar, 20 μm. ( b ) Graphs showing TUNEL-positive uninfected and infected cells. Data shown represent the mean ± SD of three independent experiments. ( c ). Specific monoclonal antibodies (mabs 1C12 for ANDV, HTNV, SEOV, PUUV and TULV, and 7B3F7 for PHV) were used for detection of N protein. <t>Calnexin</t> was used as loading control. One representative experiment out of three independent experiments is shown. Band intensity was analysed by densitometry of caspase-3 cleaved PARP and full-length PARP. The ratios in intensity between cleaved and full-length PARP were calculated and compared between infected and uninfected conditions. The ratio from uninfected cells represents maximal caspase-3 cleaved PARP/full-length PARP ratio (100%). The graphs show mean ± SD of the ratio between cleaved and full-length PARP from three experiments. Paired t-test: *p
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    Orthohantaviruses hinder staurosporine-induced apoptosis in human primary endothelial cells. ( a ) Representative immunofluorescence images from three independent experiments of ANDV-, HTNV-, SEOV-, PUUV-, PHV- and TULV-infected HUVEC after exposure to staurosporine (2 μM) for approximately 4 hours. Cells were infected at a low MOI (0.01) to achieve 20 to 30% infection at 4 days post-infection. Following staurosporine-treatment, cells were stained with TUNEL (red) to assess apoptosis, convalescent PUUV patient serum (green) to detect virus infection and DAPI (blue) for nuclear counterstaining. Scale bar, 20 μm. ( b ) Graphs showing TUNEL-positive uninfected and infected cells. Data shown represent the mean ± SD of three independent experiments. ( c ). Specific monoclonal antibodies (mabs 1C12 for ANDV, HTNV, SEOV, PUUV and TULV, and 7B3F7 for PHV) were used for detection of N protein. <t>Calnexin</t> was used as loading control. One representative experiment out of three independent experiments is shown. Band intensity was analysed by densitometry of caspase-3 cleaved PARP and full-length PARP. The ratios in intensity between cleaved and full-length PARP were calculated and compared between infected and uninfected conditions. The ratio from uninfected cells represents maximal caspase-3 cleaved PARP/full-length PARP ratio (100%). The graphs show mean ± SD of the ratio between cleaved and full-length PARP from three experiments. Paired t-test: *p
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    Orthohantaviruses hinder staurosporine-induced apoptosis in human primary endothelial cells. ( a ) Representative immunofluorescence images from three independent experiments of ANDV-, HTNV-, SEOV-, PUUV-, PHV- and TULV-infected HUVEC after exposure to staurosporine (2 μM) for approximately 4 hours. Cells were infected at a low MOI (0.01) to achieve 20 to 30% infection at 4 days post-infection. Following staurosporine-treatment, cells were stained with TUNEL (red) to assess apoptosis, convalescent PUUV patient serum (green) to detect virus infection and DAPI (blue) for nuclear counterstaining. Scale bar, 20 μm. ( b ) Graphs showing TUNEL-positive uninfected and infected cells. Data shown represent the mean ± SD of three independent experiments. ( c ). Specific monoclonal antibodies (mabs 1C12 for ANDV, HTNV, SEOV, PUUV and TULV, and 7B3F7 for PHV) were used for detection of N protein. <t>Calnexin</t> was used as loading control. One representative experiment out of three independent experiments is shown. Band intensity was analysed by densitometry of caspase-3 cleaved PARP and full-length PARP. The ratios in intensity between cleaved and full-length PARP were calculated and compared between infected and uninfected conditions. The ratio from uninfected cells represents maximal caspase-3 cleaved PARP/full-length PARP ratio (100%). The graphs show mean ± SD of the ratio between cleaved and full-length PARP from three experiments. Paired t-test: *p
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    Image Search Results


    Orthohantaviruses hinder staurosporine-induced apoptosis in human primary endothelial cells. ( a ) Representative immunofluorescence images from three independent experiments of ANDV-, HTNV-, SEOV-, PUUV-, PHV- and TULV-infected HUVEC after exposure to staurosporine (2 μM) for approximately 4 hours. Cells were infected at a low MOI (0.01) to achieve 20 to 30% infection at 4 days post-infection. Following staurosporine-treatment, cells were stained with TUNEL (red) to assess apoptosis, convalescent PUUV patient serum (green) to detect virus infection and DAPI (blue) for nuclear counterstaining. Scale bar, 20 μm. ( b ) Graphs showing TUNEL-positive uninfected and infected cells. Data shown represent the mean ± SD of three independent experiments. ( c ). Specific monoclonal antibodies (mabs 1C12 for ANDV, HTNV, SEOV, PUUV and TULV, and 7B3F7 for PHV) were used for detection of N protein. Calnexin was used as loading control. One representative experiment out of three independent experiments is shown. Band intensity was analysed by densitometry of caspase-3 cleaved PARP and full-length PARP. The ratios in intensity between cleaved and full-length PARP were calculated and compared between infected and uninfected conditions. The ratio from uninfected cells represents maximal caspase-3 cleaved PARP/full-length PARP ratio (100%). The graphs show mean ± SD of the ratio between cleaved and full-length PARP from three experiments. Paired t-test: *p

    Journal: Scientific Reports

    Article Title: Orthohantaviruses belonging to three phylogroups all inhibit apoptosis in infected target cells

    doi: 10.1038/s41598-018-37446-1

    Figure Lengend Snippet: Orthohantaviruses hinder staurosporine-induced apoptosis in human primary endothelial cells. ( a ) Representative immunofluorescence images from three independent experiments of ANDV-, HTNV-, SEOV-, PUUV-, PHV- and TULV-infected HUVEC after exposure to staurosporine (2 μM) for approximately 4 hours. Cells were infected at a low MOI (0.01) to achieve 20 to 30% infection at 4 days post-infection. Following staurosporine-treatment, cells were stained with TUNEL (red) to assess apoptosis, convalescent PUUV patient serum (green) to detect virus infection and DAPI (blue) for nuclear counterstaining. Scale bar, 20 μm. ( b ) Graphs showing TUNEL-positive uninfected and infected cells. Data shown represent the mean ± SD of three independent experiments. ( c ). Specific monoclonal antibodies (mabs 1C12 for ANDV, HTNV, SEOV, PUUV and TULV, and 7B3F7 for PHV) were used for detection of N protein. Calnexin was used as loading control. One representative experiment out of three independent experiments is shown. Band intensity was analysed by densitometry of caspase-3 cleaved PARP and full-length PARP. The ratios in intensity between cleaved and full-length PARP were calculated and compared between infected and uninfected conditions. The ratio from uninfected cells represents maximal caspase-3 cleaved PARP/full-length PARP ratio (100%). The graphs show mean ± SD of the ratio between cleaved and full-length PARP from three experiments. Paired t-test: *p

    Article Snippet: MAbs against PARP (#9532) and calnexin (#2679) were purchased from Cell Signaling Technology, while anti-caspase-3 antibody (#ab13585) was from Abcam.

    Techniques: Immunofluorescence, Infection, Staining, TUNEL Assay