Journal: JAC-Antimicrobial Resistance

Article Title: SAAP-148 and halicin exhibit synergistic antimicrobial activity against antimicrobial-resistant bacteria in skin but not airway epithelial culture models

doi: 10.1093/jacamr/dlaf050

Figure Lengend Snippet: Effect of SAAP-148 pretreatment, halicin treatment and combinations thereof on MRSA counts in HSE models

Article Snippet: Halicin (Tocris Bioscience, UK) was dissolved in DMSO to a concentration of 13.5 g/L and diluted in PBS to ≤54 mg/L.

Techniques:

Journal: JAC-Antimicrobial Resistance

Article Title: SAAP-148 and halicin exhibit synergistic antimicrobial activity against antimicrobial-resistant bacteria in skin but not airway epithelial culture models

doi: 10.1093/jacamr/dlaf050

Figure Lengend Snippet: Effect of SAAP-148 pretreatment, halicin treatment and combinations thereof on MRSA counts in PBEC models

Article Snippet: Halicin (Tocris Bioscience, UK) was dissolved in DMSO to a concentration of 13.5 g/L and diluted in PBS to ≤54 mg/L.

Techniques:

Journal: Antibiotics

Article Title: Synergism between the Synthetic Antibacterial and Antibiofilm Peptide (SAAP)-148 and Halicin

doi: 10.3390/antibiotics11050673

Figure Lengend Snippet: In vitro killing of planktonic AMR E. coli , A. baumannii and S. aureus by SAAP-148 and halicin.

Article Snippet: Halicin (SU 3327; 261.3 g/mol) was obtained from Tocris Bioscience (Bristol, UK).

Techniques: In Vitro

Journal: Antibiotics

Article Title: Synergism between the Synthetic Antibacterial and Antibiofilm Peptide (SAAP)-148 and Halicin

doi: 10.3390/antibiotics11050673

Figure Lengend Snippet: In vitro killing of biofilm-residing AMR E. coli , A. baumannii and S. aureus by SAAP-148 and halicin.

Article Snippet: Halicin (SU 3327; 261.3 g/mol) was obtained from Tocris Bioscience (Bristol, UK).

Techniques: In Vitro

Journal: Antibiotics

Article Title: Synergism between the Synthetic Antibacterial and Antibiofilm Peptide (SAAP)-148 and Halicin

doi: 10.3390/antibiotics11050673

Figure Lengend Snippet: Hemolytic and cytotoxic activities of SAAP-148 and halicin. ( A ) Values presented are cytotoxicity values of (i) 2% human erythrocytes in PBS or 50% plasma after 1 h exposure to SAAP-148 or halicin, (ii) a monolayer of human primary skin fibroblasts in DMEM medium with 0.5% human serum after 4 h and 24 h exposure to SAAP-148 or halicin and (iii) a monolayer of RT-4 urothelial cells in McCoy’s medium with 10% fetal calf serum after 4 h and 24 h exposure to SAAP-148 or halicin. Results are depicted as the medians (bold) and ranges of the IC 50 , i.e., the calculated concentration of the agents resulting in 50% cytotoxicity, of three independent experiments performed in triplicate. ( B ) Metabolic activity and cytotoxicity (determined by LDH release) of human primary skin fibroblasts upon exposure to SAAP-148 and halicin in DMEM medium with 0.5% human serum for 4 h (solid) and 24 h (dashed). Results are depicted as medians of three independent experiments performed in triplicate.

Article Snippet: Halicin (SU 3327; 261.3 g/mol) was obtained from Tocris Bioscience (Bristol, UK).

Techniques: Clinical Proteomics, Concentration Assay, Activity Assay

Journal: Antibiotics

Article Title: Synergism between the Synthetic Antibacterial and Antibiofilm Peptide (SAAP)-148 and Halicin

doi: 10.3390/antibiotics11050673

Figure Lengend Snippet: Synergistic and additive interactions between SAAP-148 and halicin. ( A ) fractional inhibitory concentration index (FICI) scores for exposure of planktonic cells of several bacterial strains to SAAP-148, halicin and combinations thereof. Interactions are considered synergistic or additive with a FICI score <0.5 or between 0.5 and 1, respectively, while interactions are considered indifferent or antagonistic with a FICI score between 1 and 4 or >4, respectively. Results are depicted as median and ranges of two to three (when additive effect) or three to four (when synergism) independent experiments. ( B ) Bacterial load of S. aureus (LUH14616) biofilms adhered to silicone disks after 4 h exposure to SAAP-148, halicin or combinations thereof. Results are shown as individual values and medians of at least two independent experiments performed in quadruplicate. Statistical differences between the two groups are depicted as * for p ≤ 0.1 and **** for p ≤ 0.0001 as calculated by the Mann-Whitney U-test.

Article Snippet: Halicin (SU 3327; 261.3 g/mol) was obtained from Tocris Bioscience (Bristol, UK).

Techniques: Concentration Assay, MANN-WHITNEY

Journal: Antibiotics

Article Title: Synergism between the Synthetic Antibacterial and Antibiofilm Peptide (SAAP)-148 and Halicin

doi: 10.3390/antibiotics11050673

Figure Lengend Snippet: Bactericidal effects of SAAP-148 and halicin and combinations thereof on E. coli in the luminal and cellular compartments of 3D bladder infection models. Bacterial load of E. coli (LUH15108) in ( A ) the supernatant (luminal compartment) and ( B ) the model (cellular compartment) of 3D bladder models composed of RT-4 cells after 4 h exposure to SAAP-148, halicin or combinations thereof. Results are shown as individual values and medians of three to seven independent experiments performed in duplicate. Statistical differences between the two groups are depicted as ** for p ≤ 0.01 and **** for p ≤ 0.0001 as calculated by the Mann–Whitney U-test.

Article Snippet: Halicin (SU 3327; 261.3 g/mol) was obtained from Tocris Bioscience (Bristol, UK).

Techniques: Infection, MANN-WHITNEY

Journal: Antibiotics

Article Title: Synergism between the Synthetic Antibacterial and Antibiofilm Peptide (SAAP)-148 and Halicin

doi: 10.3390/antibiotics11050673

Figure Lengend Snippet: Bactericidal effects of SAAP-148 and halicin and combinations thereof on S. aureus in the adherent fraction of 3D epidermal infection model. Bacterial load of adherent bacteria of MRSA (LUH14616) infected 3D human epidermal models after 4-h exposure to SAAP-148, halicin or combinations thereof. Results are shown as individual values and medians of three independent experiments performed in duplicate or triplicate. Statistical differences between the two groups are depicted as * for p ≤ 0.1 and *** for p ≤ 0.001 as calculated by the Mann–Whitney U-test.

Article Snippet: Halicin (SU 3327; 261.3 g/mol) was obtained from Tocris Bioscience (Bristol, UK).

Techniques: Infection, Bacteria, MANN-WHITNEY

Journal: Antibiotics

Article Title: Halicin: A New Horizon in Antibacterial Therapy against Veterinary Pathogens

doi: 10.3390/antibiotics13060492

Figure Lengend Snippet: Determination of minimum inhibitory concentration and minimum bactericidal concentration of SU3327 and commonly used antimicrobial agents against the test pathogens (20 strains each, totaling 260 strains).

Article Snippet: The time-killing curve results illustrated a concentration-dependent bactericidal effect of halicin against E. coli ATCC 25922, S. aureus ATCC 29213, and APP S6 ( ).

Techniques: Concentration Assay, Bacteria

Journal: Antibiotics

Article Title: Halicin: A New Horizon in Antibacterial Therapy against Veterinary Pathogens

doi: 10.3390/antibiotics13060492

Figure Lengend Snippet: Antimicrobial kinetic curves of halicin against E. coli ATCC 25922 ( A ), S. aureus ATCC 29213 ( B ), and APP S6 ( C ).

Article Snippet: The time-killing curve results illustrated a concentration-dependent bactericidal effect of halicin against E. coli ATCC 25922, S. aureus ATCC 29213, and APP S6 ( ).

Techniques:

Journal: Antibiotics

Article Title: Halicin: A New Horizon in Antibacterial Therapy against Veterinary Pathogens

doi: 10.3390/antibiotics13060492

Figure Lengend Snippet: The post-antibiotic effects and post-antibiotic sub-minimum inhibitory concentration effects against E. coli ATCC 25922 ( A ), S. aureus ATCC 29213 ( B ), and APP S6 (C) for halicin.

Article Snippet: The time-killing curve results illustrated a concentration-dependent bactericidal effect of halicin against E. coli ATCC 25922, S. aureus ATCC 29213, and APP S6 ( ).

Techniques: Concentration Assay

Journal: Antibiotics

Article Title: Halicin: A New Horizon in Antibacterial Therapy against Veterinary Pathogens

doi: 10.3390/antibiotics13060492

Figure Lengend Snippet: Comparison of the post-antibiotic effects and post-antibiotic sub-minimum inhibitory concentration effects of antibacterial drugs against E. coli ATCC 25922, S. aureus ATCC 29213, and APP S6.

Article Snippet: The time-killing curve results illustrated a concentration-dependent bactericidal effect of halicin against E. coli ATCC 25922, S. aureus ATCC 29213, and APP S6 ( ).

Techniques: Comparison, Concentration Assay

Journal: Antibiotics

Article Title: Halicin: A New Horizon in Antibacterial Therapy against Veterinary Pathogens

doi: 10.3390/antibiotics13060492

Figure Lengend Snippet: Evolution of resistance to halicin and control drugs in E. coli ATCC 25922 ( A ), S. aureus ATCC 29213 ( B ), and APP S6 ( C ) after 40 d of passaging.

Article Snippet: The time-killing curve results illustrated a concentration-dependent bactericidal effect of halicin against E. coli ATCC 25922, S. aureus ATCC 29213, and APP S6 ( ).

Techniques: Control, Passaging

Journal: Antibiotics

Article Title: Halicin: A New Horizon in Antibacterial Therapy against Veterinary Pathogens

doi: 10.3390/antibiotics13060492

Figure Lengend Snippet: The minimum preventable concentrations and mutant selection windows for halicin and control drugs against E. coli ATCC 25922, S. aureus ATCC 29213, and APP S6.

Article Snippet: The time-killing curve results illustrated a concentration-dependent bactericidal effect of halicin against E. coli ATCC 25922, S. aureus ATCC 29213, and APP S6 ( ).

Techniques: Mutagenesis, Selection, Control

Journal: Antibiotics

Article Title: Assessment of the Antibacterial Efficacy of Halicin against Pathogenic Bacteria

doi: 10.3390/antibiotics10121480

Figure Lengend Snippet: The line charts for minimum inhibitory concentration (MIC) values of fresh and old halicin against four types of bacteria showing the efficiency of halicin. The MICs of freshly prepared halicin were measured at 16 μg/mL for S. aureus ATCC BAA-977 ( A ), 32 μg/mL for E. coli ATCC 25922 ( B ), 128 μg/mL for A. baumannii ATCC BAA-747 ( C ), and 256 μg/mL for MDR A. baumannii MDR 3086 ( D ). In comparison, they doubled for the old-prepared halicin, which increased to be 32 μg/mL for S. aureus , 64 μg/mL for E. coli , 128 μg/mL for A. baumannii , and ≥256 μg/mL for MDR A. baumannii.

Article Snippet: Halicin was purchased from Carbosynth (Carbosynth Ltd., Compton, UK), while Mueller-Hinton broth was bought from Scharlab (Scharlab, S.L., Barcelona, Spain).

Techniques: Concentration Assay, Bacteria, Comparison

Journal: Antibiotics

Article Title: Assessment of the Antibacterial Efficacy of Halicin against Pathogenic Bacteria

doi: 10.3390/antibiotics10121480

Figure Lengend Snippet: The line charts for the coefficient of determination (R 2 ) values show the efficiency of halicin against four types of bacterial strains. The R 2 of halicin was generally considered strong (R 2 ≥ 0.9) against E. coli ATCC 25922 ( B ; R 2 = 0.9544) and A. baumannii ATCC BAA-747 ( C ; R 2 = 0.9524), and fairly strong (R 2 ≥ 0.8 and <0.9) against S. aureus ATCC BAA-977 ( A ; R 2 = 0.8087) and A. baumannii MDR 3086 ( D ; R 2 = 0.8717).

Article Snippet: Halicin was purchased from Carbosynth (Carbosynth Ltd., Compton, UK), while Mueller-Hinton broth was bought from Scharlab (Scharlab, S.L., Barcelona, Spain).

Techniques: