h.pylori infection serum immunoglobulin ig g antibodies Search Results


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  • 99
    Millipore human anti igg
    Functional effect of <t>anti-PDI</t> antibodies on PDI activity and association of anti-PDI status with insulin resistance. ( A ) Effects of purified anti-PDI antibodies on purified bovine PDI activity. Purified Bovine PDI activity was measured in the presence of 0–60 µg/ml purified anti- bovine PDI from anti-PDI positive subjects (triangles) and 0–160 µg/ml total <t>IgG</t> from rabbit immuned against bovine PDI (diamonds). Pooled human and rabbit IgG (dashed lines) were used as control. Results are compared to activity in the presence of same volume of PBS (defined as 100%) and presented as a mean ± SEM of three experiments. PDI activity was measured using the insulin transhydrogenase assay. ( B ) Effects of human sera (1∶50) on human recombinant PDI activity. Recombinant human PDI activity was measured in the presence of 20 µl/ml human sera positive or negative for anti-human PDI antibody. Results are presented as mean ± SEM. ( C ) Association of anti- human PDI status with serum insulin levels. Results are presented as mean ± SEM, adjusted for age, gender, education, BMI and smoking. ( D ) Association of anti-human PDI status with insulin resistance. Insulin resistance was estimated using HOMA (Homeostatic Model Assessment of insulin resistance). Results are presented as mean ± SEM, adjusted for age, gender, education, BMI and smoking. For details, see Table S2 .
    Human Anti Igg, supplied by Millipore, used in various techniques. Bioz Stars score: 99/100, based on 140 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    Trinity Biotech pylori immunoglobulin g
    Functional effect of <t>anti-PDI</t> antibodies on PDI activity and association of anti-PDI status with insulin resistance. ( A ) Effects of purified anti-PDI antibodies on purified bovine PDI activity. Purified Bovine PDI activity was measured in the presence of 0–60 µg/ml purified anti- bovine PDI from anti-PDI positive subjects (triangles) and 0–160 µg/ml total <t>IgG</t> from rabbit immuned against bovine PDI (diamonds). Pooled human and rabbit IgG (dashed lines) were used as control. Results are compared to activity in the presence of same volume of PBS (defined as 100%) and presented as a mean ± SEM of three experiments. PDI activity was measured using the insulin transhydrogenase assay. ( B ) Effects of human sera (1∶50) on human recombinant PDI activity. Recombinant human PDI activity was measured in the presence of 20 µl/ml human sera positive or negative for anti-human PDI antibody. Results are presented as mean ± SEM. ( C ) Association of anti- human PDI status with serum insulin levels. Results are presented as mean ± SEM, adjusted for age, gender, education, BMI and smoking. ( D ) Association of anti-human PDI status with insulin resistance. Insulin resistance was estimated using HOMA (Homeostatic Model Assessment of insulin resistance). Results are presented as mean ± SEM, adjusted for age, gender, education, BMI and smoking. For details, see Table S2 .
    Pylori Immunoglobulin G, supplied by Trinity Biotech, used in various techniques. Bioz Stars score: 88/100, based on 4 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    Dade Behring pylori immunoglobulin g antibodies
    Functional effect of <t>anti-PDI</t> antibodies on PDI activity and association of anti-PDI status with insulin resistance. ( A ) Effects of purified anti-PDI antibodies on purified bovine PDI activity. Purified Bovine PDI activity was measured in the presence of 0–60 µg/ml purified anti- bovine PDI from anti-PDI positive subjects (triangles) and 0–160 µg/ml total <t>IgG</t> from rabbit immuned against bovine PDI (diamonds). Pooled human and rabbit IgG (dashed lines) were used as control. Results are compared to activity in the presence of same volume of PBS (defined as 100%) and presented as a mean ± SEM of three experiments. PDI activity was measured using the insulin transhydrogenase assay. ( B ) Effects of human sera (1∶50) on human recombinant PDI activity. Recombinant human PDI activity was measured in the presence of 20 µl/ml human sera positive or negative for anti-human PDI antibody. Results are presented as mean ± SEM. ( C ) Association of anti- human PDI status with serum insulin levels. Results are presented as mean ± SEM, adjusted for age, gender, education, BMI and smoking. ( D ) Association of anti-human PDI status with insulin resistance. Insulin resistance was estimated using HOMA (Homeostatic Model Assessment of insulin resistance). Results are presented as mean ± SEM, adjusted for age, gender, education, BMI and smoking. For details, see Table S2 .
    Pylori Immunoglobulin G Antibodies, supplied by Dade Behring, used in various techniques. Bioz Stars score: 85/100, based on 3 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    Agilent technologies pylori immune serum
    Functional effect of <t>anti-PDI</t> antibodies on PDI activity and association of anti-PDI status with insulin resistance. ( A ) Effects of purified anti-PDI antibodies on purified bovine PDI activity. Purified Bovine PDI activity was measured in the presence of 0–60 µg/ml purified anti- bovine PDI from anti-PDI positive subjects (triangles) and 0–160 µg/ml total <t>IgG</t> from rabbit immuned against bovine PDI (diamonds). Pooled human and rabbit IgG (dashed lines) were used as control. Results are compared to activity in the presence of same volume of PBS (defined as 100%) and presented as a mean ± SEM of three experiments. PDI activity was measured using the insulin transhydrogenase assay. ( B ) Effects of human sera (1∶50) on human recombinant PDI activity. Recombinant human PDI activity was measured in the presence of 20 µl/ml human sera positive or negative for anti-human PDI antibody. Results are presented as mean ± SEM. ( C ) Association of anti- human PDI status with serum insulin levels. Results are presented as mean ± SEM, adjusted for age, gender, education, BMI and smoking. ( D ) Association of anti-human PDI status with insulin resistance. Insulin resistance was estimated using HOMA (Homeostatic Model Assessment of insulin resistance). Results are presented as mean ± SEM, adjusted for age, gender, education, BMI and smoking. For details, see Table S2 .
    Pylori Immune Serum, supplied by Agilent technologies, used in various techniques. Bioz Stars score: 85/100, based on 2 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    90
    Eiken Chemical serum immunoglobulin g antibody test
    Functional effect of <t>anti-PDI</t> antibodies on PDI activity and association of anti-PDI status with insulin resistance. ( A ) Effects of purified anti-PDI antibodies on purified bovine PDI activity. Purified Bovine PDI activity was measured in the presence of 0–60 µg/ml purified anti- bovine PDI from anti-PDI positive subjects (triangles) and 0–160 µg/ml total <t>IgG</t> from rabbit immuned against bovine PDI (diamonds). Pooled human and rabbit IgG (dashed lines) were used as control. Results are compared to activity in the presence of same volume of PBS (defined as 100%) and presented as a mean ± SEM of three experiments. PDI activity was measured using the insulin transhydrogenase assay. ( B ) Effects of human sera (1∶50) on human recombinant PDI activity. Recombinant human PDI activity was measured in the presence of 20 µl/ml human sera positive or negative for anti-human PDI antibody. Results are presented as mean ± SEM. ( C ) Association of anti- human PDI status with serum insulin levels. Results are presented as mean ± SEM, adjusted for age, gender, education, BMI and smoking. ( D ) Association of anti-human PDI status with insulin resistance. Insulin resistance was estimated using HOMA (Homeostatic Model Assessment of insulin resistance). Results are presented as mean ± SEM, adjusted for age, gender, education, BMI and smoking. For details, see Table S2 .
    Serum Immunoglobulin G Antibody Test, supplied by Eiken Chemical, used in various techniques. Bioz Stars score: 90/100, based on 3 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    Green Cross Inc pylori immunoglobulin g
    Functional effect of <t>anti-PDI</t> antibodies on PDI activity and association of anti-PDI status with insulin resistance. ( A ) Effects of purified anti-PDI antibodies on purified bovine PDI activity. Purified Bovine PDI activity was measured in the presence of 0–60 µg/ml purified anti- bovine PDI from anti-PDI positive subjects (triangles) and 0–160 µg/ml total <t>IgG</t> from rabbit immuned against bovine PDI (diamonds). Pooled human and rabbit IgG (dashed lines) were used as control. Results are compared to activity in the presence of same volume of PBS (defined as 100%) and presented as a mean ± SEM of three experiments. PDI activity was measured using the insulin transhydrogenase assay. ( B ) Effects of human sera (1∶50) on human recombinant PDI activity. Recombinant human PDI activity was measured in the presence of 20 µl/ml human sera positive or negative for anti-human PDI antibody. Results are presented as mean ± SEM. ( C ) Association of anti- human PDI status with serum insulin levels. Results are presented as mean ± SEM, adjusted for age, gender, education, BMI and smoking. ( D ) Association of anti-human PDI status with insulin resistance. Insulin resistance was estimated using HOMA (Homeostatic Model Assessment of insulin resistance). Results are presented as mean ± SEM, adjusted for age, gender, education, BMI and smoking. For details, see Table S2 .
    Pylori Immunoglobulin G, supplied by Green Cross Inc, used in various techniques. Bioz Stars score: 88/100, based on 35 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    Jackson Immuno mouse immunoglobulins
    Functional effect of <t>anti-PDI</t> antibodies on PDI activity and association of anti-PDI status with insulin resistance. ( A ) Effects of purified anti-PDI antibodies on purified bovine PDI activity. Purified Bovine PDI activity was measured in the presence of 0–60 µg/ml purified anti- bovine PDI from anti-PDI positive subjects (triangles) and 0–160 µg/ml total <t>IgG</t> from rabbit immuned against bovine PDI (diamonds). Pooled human and rabbit IgG (dashed lines) were used as control. Results are compared to activity in the presence of same volume of PBS (defined as 100%) and presented as a mean ± SEM of three experiments. PDI activity was measured using the insulin transhydrogenase assay. ( B ) Effects of human sera (1∶50) on human recombinant PDI activity. Recombinant human PDI activity was measured in the presence of 20 µl/ml human sera positive or negative for anti-human PDI antibody. Results are presented as mean ± SEM. ( C ) Association of anti- human PDI status with serum insulin levels. Results are presented as mean ± SEM, adjusted for age, gender, education, BMI and smoking. ( D ) Association of anti-human PDI status with insulin resistance. Insulin resistance was estimated using HOMA (Homeostatic Model Assessment of insulin resistance). Results are presented as mean ± SEM, adjusted for age, gender, education, BMI and smoking. For details, see Table S2 .
    Mouse Immunoglobulins, supplied by Jackson Immuno, used in various techniques. Bioz Stars score: 91/100, based on 68 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    Agilent technologies anti mouse immunoglobulin
    Functional effect of <t>anti-PDI</t> antibodies on PDI activity and association of anti-PDI status with insulin resistance. ( A ) Effects of purified anti-PDI antibodies on purified bovine PDI activity. Purified Bovine PDI activity was measured in the presence of 0–60 µg/ml purified anti- bovine PDI from anti-PDI positive subjects (triangles) and 0–160 µg/ml total <t>IgG</t> from rabbit immuned against bovine PDI (diamonds). Pooled human and rabbit IgG (dashed lines) were used as control. Results are compared to activity in the presence of same volume of PBS (defined as 100%) and presented as a mean ± SEM of three experiments. PDI activity was measured using the insulin transhydrogenase assay. ( B ) Effects of human sera (1∶50) on human recombinant PDI activity. Recombinant human PDI activity was measured in the presence of 20 µl/ml human sera positive or negative for anti-human PDI antibody. Results are presented as mean ± SEM. ( C ) Association of anti- human PDI status with serum insulin levels. Results are presented as mean ± SEM, adjusted for age, gender, education, BMI and smoking. ( D ) Association of anti-human PDI status with insulin resistance. Insulin resistance was estimated using HOMA (Homeostatic Model Assessment of insulin resistance). Results are presented as mean ± SEM, adjusted for age, gender, education, BMI and smoking. For details, see Table S2 .
    Anti Mouse Immunoglobulin, supplied by Agilent technologies, used in various techniques. Bioz Stars score: 92/100, based on 166 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    Otsuka Holdings pylori immunoglobulin g igg antibody titer
    Functional effect of <t>anti-PDI</t> antibodies on PDI activity and association of anti-PDI status with insulin resistance. ( A ) Effects of purified anti-PDI antibodies on purified bovine PDI activity. Purified Bovine PDI activity was measured in the presence of 0–60 µg/ml purified anti- bovine PDI from anti-PDI positive subjects (triangles) and 0–160 µg/ml total <t>IgG</t> from rabbit immuned against bovine PDI (diamonds). Pooled human and rabbit IgG (dashed lines) were used as control. Results are compared to activity in the presence of same volume of PBS (defined as 100%) and presented as a mean ± SEM of three experiments. PDI activity was measured using the insulin transhydrogenase assay. ( B ) Effects of human sera (1∶50) on human recombinant PDI activity. Recombinant human PDI activity was measured in the presence of 20 µl/ml human sera positive or negative for anti-human PDI antibody. Results are presented as mean ± SEM. ( C ) Association of anti- human PDI status with serum insulin levels. Results are presented as mean ± SEM, adjusted for age, gender, education, BMI and smoking. ( D ) Association of anti-human PDI status with insulin resistance. Insulin resistance was estimated using HOMA (Homeostatic Model Assessment of insulin resistance). Results are presented as mean ± SEM, adjusted for age, gender, education, BMI and smoking. For details, see Table S2 .
    Pylori Immunoglobulin G Igg Antibody Titer, supplied by Otsuka Holdings, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    Agilent technologies peroxidase conjugated antimouse immunoglobulins
    Functional effect of <t>anti-PDI</t> antibodies on PDI activity and association of anti-PDI status with insulin resistance. ( A ) Effects of purified anti-PDI antibodies on purified bovine PDI activity. Purified Bovine PDI activity was measured in the presence of 0–60 µg/ml purified anti- bovine PDI from anti-PDI positive subjects (triangles) and 0–160 µg/ml total <t>IgG</t> from rabbit immuned against bovine PDI (diamonds). Pooled human and rabbit IgG (dashed lines) were used as control. Results are compared to activity in the presence of same volume of PBS (defined as 100%) and presented as a mean ± SEM of three experiments. PDI activity was measured using the insulin transhydrogenase assay. ( B ) Effects of human sera (1∶50) on human recombinant PDI activity. Recombinant human PDI activity was measured in the presence of 20 µl/ml human sera positive or negative for anti-human PDI antibody. Results are presented as mean ± SEM. ( C ) Association of anti- human PDI status with serum insulin levels. Results are presented as mean ± SEM, adjusted for age, gender, education, BMI and smoking. ( D ) Association of anti-human PDI status with insulin resistance. Insulin resistance was estimated using HOMA (Homeostatic Model Assessment of insulin resistance). Results are presented as mean ± SEM, adjusted for age, gender, education, BMI and smoking. For details, see Table S2 .
    Peroxidase Conjugated Antimouse Immunoglobulins, supplied by Agilent technologies, used in various techniques. Bioz Stars score: 85/100, based on 2 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    R&D Systems control immunoglobulin g igg antibody
    Functional effect of <t>anti-PDI</t> antibodies on PDI activity and association of anti-PDI status with insulin resistance. ( A ) Effects of purified anti-PDI antibodies on purified bovine PDI activity. Purified Bovine PDI activity was measured in the presence of 0–60 µg/ml purified anti- bovine PDI from anti-PDI positive subjects (triangles) and 0–160 µg/ml total <t>IgG</t> from rabbit immuned against bovine PDI (diamonds). Pooled human and rabbit IgG (dashed lines) were used as control. Results are compared to activity in the presence of same volume of PBS (defined as 100%) and presented as a mean ± SEM of three experiments. PDI activity was measured using the insulin transhydrogenase assay. ( B ) Effects of human sera (1∶50) on human recombinant PDI activity. Recombinant human PDI activity was measured in the presence of 20 µl/ml human sera positive or negative for anti-human PDI antibody. Results are presented as mean ± SEM. ( C ) Association of anti- human PDI status with serum insulin levels. Results are presented as mean ± SEM, adjusted for age, gender, education, BMI and smoking. ( D ) Association of anti-human PDI status with insulin resistance. Insulin resistance was estimated using HOMA (Homeostatic Model Assessment of insulin resistance). Results are presented as mean ± SEM, adjusted for age, gender, education, BMI and smoking. For details, see Table S2 .
    Control Immunoglobulin G Igg Antibody, supplied by R&D Systems, used in various techniques. Bioz Stars score: 99/100, based on 23 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    Agilent technologies pylori antibody
    Functional effect of <t>anti-PDI</t> antibodies on PDI activity and association of anti-PDI status with insulin resistance. ( A ) Effects of purified anti-PDI antibodies on purified bovine PDI activity. Purified Bovine PDI activity was measured in the presence of 0–60 µg/ml purified anti- bovine PDI from anti-PDI positive subjects (triangles) and 0–160 µg/ml total <t>IgG</t> from rabbit immuned against bovine PDI (diamonds). Pooled human and rabbit IgG (dashed lines) were used as control. Results are compared to activity in the presence of same volume of PBS (defined as 100%) and presented as a mean ± SEM of three experiments. PDI activity was measured using the insulin transhydrogenase assay. ( B ) Effects of human sera (1∶50) on human recombinant PDI activity. Recombinant human PDI activity was measured in the presence of 20 µl/ml human sera positive or negative for anti-human PDI antibody. Results are presented as mean ± SEM. ( C ) Association of anti- human PDI status with serum insulin levels. Results are presented as mean ± SEM, adjusted for age, gender, education, BMI and smoking. ( D ) Association of anti-human PDI status with insulin resistance. Insulin resistance was estimated using HOMA (Homeostatic Model Assessment of insulin resistance). Results are presented as mean ± SEM, adjusted for age, gender, education, BMI and smoking. For details, see Table S2 .
    Pylori Antibody, supplied by Agilent technologies, used in various techniques. Bioz Stars score: 90/100, based on 245 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    R&D Systems mouse immunoglobulin g2a
    Functional effect of <t>anti-PDI</t> antibodies on PDI activity and association of anti-PDI status with insulin resistance. ( A ) Effects of purified anti-PDI antibodies on purified bovine PDI activity. Purified Bovine PDI activity was measured in the presence of 0–60 µg/ml purified anti- bovine PDI from anti-PDI positive subjects (triangles) and 0–160 µg/ml total <t>IgG</t> from rabbit immuned against bovine PDI (diamonds). Pooled human and rabbit IgG (dashed lines) were used as control. Results are compared to activity in the presence of same volume of PBS (defined as 100%) and presented as a mean ± SEM of three experiments. PDI activity was measured using the insulin transhydrogenase assay. ( B ) Effects of human sera (1∶50) on human recombinant PDI activity. Recombinant human PDI activity was measured in the presence of 20 µl/ml human sera positive or negative for anti-human PDI antibody. Results are presented as mean ± SEM. ( C ) Association of anti- human PDI status with serum insulin levels. Results are presented as mean ± SEM, adjusted for age, gender, education, BMI and smoking. ( D ) Association of anti-human PDI status with insulin resistance. Insulin resistance was estimated using HOMA (Homeostatic Model Assessment of insulin resistance). Results are presented as mean ± SEM, adjusted for age, gender, education, BMI and smoking. For details, see Table S2 .
    Mouse Immunoglobulin G2a, supplied by R&D Systems, used in various techniques. Bioz Stars score: 97/100, based on 20 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    Roche immunoglobulin g igg antibodies
    Functional effect of <t>anti-PDI</t> antibodies on PDI activity and association of anti-PDI status with insulin resistance. ( A ) Effects of purified anti-PDI antibodies on purified bovine PDI activity. Purified Bovine PDI activity was measured in the presence of 0–60 µg/ml purified anti- bovine PDI from anti-PDI positive subjects (triangles) and 0–160 µg/ml total <t>IgG</t> from rabbit immuned against bovine PDI (diamonds). Pooled human and rabbit IgG (dashed lines) were used as control. Results are compared to activity in the presence of same volume of PBS (defined as 100%) and presented as a mean ± SEM of three experiments. PDI activity was measured using the insulin transhydrogenase assay. ( B ) Effects of human sera (1∶50) on human recombinant PDI activity. Recombinant human PDI activity was measured in the presence of 20 µl/ml human sera positive or negative for anti-human PDI antibody. Results are presented as mean ± SEM. ( C ) Association of anti- human PDI status with serum insulin levels. Results are presented as mean ± SEM, adjusted for age, gender, education, BMI and smoking. ( D ) Association of anti-human PDI status with insulin resistance. Insulin resistance was estimated using HOMA (Homeostatic Model Assessment of insulin resistance). Results are presented as mean ± SEM, adjusted for age, gender, education, BMI and smoking. For details, see Table S2 .
    Immunoglobulin G Igg Antibodies, supplied by Roche, used in various techniques. Bioz Stars score: 88/100, based on 3 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    Dianova biotinylated goat anti human immunoglobulin
    Functional effect of <t>anti-PDI</t> antibodies on PDI activity and association of anti-PDI status with insulin resistance. ( A ) Effects of purified anti-PDI antibodies on purified bovine PDI activity. Purified Bovine PDI activity was measured in the presence of 0–60 µg/ml purified anti- bovine PDI from anti-PDI positive subjects (triangles) and 0–160 µg/ml total <t>IgG</t> from rabbit immuned against bovine PDI (diamonds). Pooled human and rabbit IgG (dashed lines) were used as control. Results are compared to activity in the presence of same volume of PBS (defined as 100%) and presented as a mean ± SEM of three experiments. PDI activity was measured using the insulin transhydrogenase assay. ( B ) Effects of human sera (1∶50) on human recombinant PDI activity. Recombinant human PDI activity was measured in the presence of 20 µl/ml human sera positive or negative for anti-human PDI antibody. Results are presented as mean ± SEM. ( C ) Association of anti- human PDI status with serum insulin levels. Results are presented as mean ± SEM, adjusted for age, gender, education, BMI and smoking. ( D ) Association of anti-human PDI status with insulin resistance. Insulin resistance was estimated using HOMA (Homeostatic Model Assessment of insulin resistance). Results are presented as mean ± SEM, adjusted for age, gender, education, BMI and smoking. For details, see Table S2 .
    Biotinylated Goat Anti Human Immunoglobulin, supplied by Dianova, used in various techniques. Bioz Stars score: 88/100, based on 7 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    Eiken Chemical pylori igg antibody
    Functional effect of <t>anti-PDI</t> antibodies on PDI activity and association of anti-PDI status with insulin resistance. ( A ) Effects of purified anti-PDI antibodies on purified bovine PDI activity. Purified Bovine PDI activity was measured in the presence of 0–60 µg/ml purified anti- bovine PDI from anti-PDI positive subjects (triangles) and 0–160 µg/ml total <t>IgG</t> from rabbit immuned against bovine PDI (diamonds). Pooled human and rabbit IgG (dashed lines) were used as control. Results are compared to activity in the presence of same volume of PBS (defined as 100%) and presented as a mean ± SEM of three experiments. PDI activity was measured using the insulin transhydrogenase assay. ( B ) Effects of human sera (1∶50) on human recombinant PDI activity. Recombinant human PDI activity was measured in the presence of 20 µl/ml human sera positive or negative for anti-human PDI antibody. Results are presented as mean ± SEM. ( C ) Association of anti- human PDI status with serum insulin levels. Results are presented as mean ± SEM, adjusted for age, gender, education, BMI and smoking. ( D ) Association of anti-human PDI status with insulin resistance. Insulin resistance was estimated using HOMA (Homeostatic Model Assessment of insulin resistance). Results are presented as mean ± SEM, adjusted for age, gender, education, BMI and smoking. For details, see Table S2 .
    Pylori Igg Antibody, supplied by Eiken Chemical, used in various techniques. Bioz Stars score: 88/100, based on 11 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    American Qualex peroxidase labeled anti human immunoglobulin γ chain f
    Functional effect of <t>anti-PDI</t> antibodies on PDI activity and association of anti-PDI status with insulin resistance. ( A ) Effects of purified anti-PDI antibodies on purified bovine PDI activity. Purified Bovine PDI activity was measured in the presence of 0–60 µg/ml purified anti- bovine PDI from anti-PDI positive subjects (triangles) and 0–160 µg/ml total <t>IgG</t> from rabbit immuned against bovine PDI (diamonds). Pooled human and rabbit IgG (dashed lines) were used as control. Results are compared to activity in the presence of same volume of PBS (defined as 100%) and presented as a mean ± SEM of three experiments. PDI activity was measured using the insulin transhydrogenase assay. ( B ) Effects of human sera (1∶50) on human recombinant PDI activity. Recombinant human PDI activity was measured in the presence of 20 µl/ml human sera positive or negative for anti-human PDI antibody. Results are presented as mean ± SEM. ( C ) Association of anti- human PDI status with serum insulin levels. Results are presented as mean ± SEM, adjusted for age, gender, education, BMI and smoking. ( D ) Association of anti-human PDI status with insulin resistance. Insulin resistance was estimated using HOMA (Homeostatic Model Assessment of insulin resistance). Results are presented as mean ± SEM, adjusted for age, gender, education, BMI and smoking. For details, see Table S2 .
    Peroxidase Labeled Anti Human Immunoglobulin γ Chain F, supplied by American Qualex, used in various techniques. Bioz Stars score: 80/100, based on 4 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    Functional effect of <t>anti-PDI</t> antibodies on PDI activity and association of anti-PDI status with insulin resistance. ( A ) Effects of purified anti-PDI antibodies on purified bovine PDI activity. Purified Bovine PDI activity was measured in the presence of 0–60 µg/ml purified anti- bovine PDI from anti-PDI positive subjects (triangles) and 0–160 µg/ml total <t>IgG</t> from rabbit immuned against bovine PDI (diamonds). Pooled human and rabbit IgG (dashed lines) were used as control. Results are compared to activity in the presence of same volume of PBS (defined as 100%) and presented as a mean ± SEM of three experiments. PDI activity was measured using the insulin transhydrogenase assay. ( B ) Effects of human sera (1∶50) on human recombinant PDI activity. Recombinant human PDI activity was measured in the presence of 20 µl/ml human sera positive or negative for anti-human PDI antibody. Results are presented as mean ± SEM. ( C ) Association of anti- human PDI status with serum insulin levels. Results are presented as mean ± SEM, adjusted for age, gender, education, BMI and smoking. ( D ) Association of anti-human PDI status with insulin resistance. Insulin resistance was estimated using HOMA (Homeostatic Model Assessment of insulin resistance). Results are presented as mean ± SEM, adjusted for age, gender, education, BMI and smoking. For details, see Table S2 .
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    Functional effect of <t>anti-PDI</t> antibodies on PDI activity and association of anti-PDI status with insulin resistance. ( A ) Effects of purified anti-PDI antibodies on purified bovine PDI activity. Purified Bovine PDI activity was measured in the presence of 0–60 µg/ml purified anti- bovine PDI from anti-PDI positive subjects (triangles) and 0–160 µg/ml total <t>IgG</t> from rabbit immuned against bovine PDI (diamonds). Pooled human and rabbit IgG (dashed lines) were used as control. Results are compared to activity in the presence of same volume of PBS (defined as 100%) and presented as a mean ± SEM of three experiments. PDI activity was measured using the insulin transhydrogenase assay. ( B ) Effects of human sera (1∶50) on human recombinant PDI activity. Recombinant human PDI activity was measured in the presence of 20 µl/ml human sera positive or negative for anti-human PDI antibody. Results are presented as mean ± SEM. ( C ) Association of anti- human PDI status with serum insulin levels. Results are presented as mean ± SEM, adjusted for age, gender, education, BMI and smoking. ( D ) Association of anti-human PDI status with insulin resistance. Insulin resistance was estimated using HOMA (Homeostatic Model Assessment of insulin resistance). Results are presented as mean ± SEM, adjusted for age, gender, education, BMI and smoking. For details, see Table S2 .
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    Functional effect of <t>anti-PDI</t> antibodies on PDI activity and association of anti-PDI status with insulin resistance. ( A ) Effects of purified anti-PDI antibodies on purified bovine PDI activity. Purified Bovine PDI activity was measured in the presence of 0–60 µg/ml purified anti- bovine PDI from anti-PDI positive subjects (triangles) and 0–160 µg/ml total <t>IgG</t> from rabbit immuned against bovine PDI (diamonds). Pooled human and rabbit IgG (dashed lines) were used as control. Results are compared to activity in the presence of same volume of PBS (defined as 100%) and presented as a mean ± SEM of three experiments. PDI activity was measured using the insulin transhydrogenase assay. ( B ) Effects of human sera (1∶50) on human recombinant PDI activity. Recombinant human PDI activity was measured in the presence of 20 µl/ml human sera positive or negative for anti-human PDI antibody. Results are presented as mean ± SEM. ( C ) Association of anti- human PDI status with serum insulin levels. Results are presented as mean ± SEM, adjusted for age, gender, education, BMI and smoking. ( D ) Association of anti-human PDI status with insulin resistance. Insulin resistance was estimated using HOMA (Homeostatic Model Assessment of insulin resistance). Results are presented as mean ± SEM, adjusted for age, gender, education, BMI and smoking. For details, see Table S2 .
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    Functional effect of <t>anti-PDI</t> antibodies on PDI activity and association of anti-PDI status with insulin resistance. ( A ) Effects of purified anti-PDI antibodies on purified bovine PDI activity. Purified Bovine PDI activity was measured in the presence of 0–60 µg/ml purified anti- bovine PDI from anti-PDI positive subjects (triangles) and 0–160 µg/ml total <t>IgG</t> from rabbit immuned against bovine PDI (diamonds). Pooled human and rabbit IgG (dashed lines) were used as control. Results are compared to activity in the presence of same volume of PBS (defined as 100%) and presented as a mean ± SEM of three experiments. PDI activity was measured using the insulin transhydrogenase assay. ( B ) Effects of human sera (1∶50) on human recombinant PDI activity. Recombinant human PDI activity was measured in the presence of 20 µl/ml human sera positive or negative for anti-human PDI antibody. Results are presented as mean ± SEM. ( C ) Association of anti- human PDI status with serum insulin levels. Results are presented as mean ± SEM, adjusted for age, gender, education, BMI and smoking. ( D ) Association of anti-human PDI status with insulin resistance. Insulin resistance was estimated using HOMA (Homeostatic Model Assessment of insulin resistance). Results are presented as mean ± SEM, adjusted for age, gender, education, BMI and smoking. For details, see Table S2 .
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    Functional effect of <t>anti-PDI</t> antibodies on PDI activity and association of anti-PDI status with insulin resistance. ( A ) Effects of purified anti-PDI antibodies on purified bovine PDI activity. Purified Bovine PDI activity was measured in the presence of 0–60 µg/ml purified anti- bovine PDI from anti-PDI positive subjects (triangles) and 0–160 µg/ml total <t>IgG</t> from rabbit immuned against bovine PDI (diamonds). Pooled human and rabbit IgG (dashed lines) were used as control. Results are compared to activity in the presence of same volume of PBS (defined as 100%) and presented as a mean ± SEM of three experiments. PDI activity was measured using the insulin transhydrogenase assay. ( B ) Effects of human sera (1∶50) on human recombinant PDI activity. Recombinant human PDI activity was measured in the presence of 20 µl/ml human sera positive or negative for anti-human PDI antibody. Results are presented as mean ± SEM. ( C ) Association of anti- human PDI status with serum insulin levels. Results are presented as mean ± SEM, adjusted for age, gender, education, BMI and smoking. ( D ) Association of anti-human PDI status with insulin resistance. Insulin resistance was estimated using HOMA (Homeostatic Model Assessment of insulin resistance). Results are presented as mean ± SEM, adjusted for age, gender, education, BMI and smoking. For details, see Table S2 .
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    Image Search Results


    Functional effect of anti-PDI antibodies on PDI activity and association of anti-PDI status with insulin resistance. ( A ) Effects of purified anti-PDI antibodies on purified bovine PDI activity. Purified Bovine PDI activity was measured in the presence of 0–60 µg/ml purified anti- bovine PDI from anti-PDI positive subjects (triangles) and 0–160 µg/ml total IgG from rabbit immuned against bovine PDI (diamonds). Pooled human and rabbit IgG (dashed lines) were used as control. Results are compared to activity in the presence of same volume of PBS (defined as 100%) and presented as a mean ± SEM of three experiments. PDI activity was measured using the insulin transhydrogenase assay. ( B ) Effects of human sera (1∶50) on human recombinant PDI activity. Recombinant human PDI activity was measured in the presence of 20 µl/ml human sera positive or negative for anti-human PDI antibody. Results are presented as mean ± SEM. ( C ) Association of anti- human PDI status with serum insulin levels. Results are presented as mean ± SEM, adjusted for age, gender, education, BMI and smoking. ( D ) Association of anti-human PDI status with insulin resistance. Insulin resistance was estimated using HOMA (Homeostatic Model Assessment of insulin resistance). Results are presented as mean ± SEM, adjusted for age, gender, education, BMI and smoking. For details, see Table S2 .

    Journal: PLoS ONE

    Article Title: Post-Streptococcal Auto-Antibodies Inhibit Protein Disulfide Isomerase and Are Associated with Insulin Resistance

    doi: 10.1371/journal.pone.0012875

    Figure Lengend Snippet: Functional effect of anti-PDI antibodies on PDI activity and association of anti-PDI status with insulin resistance. ( A ) Effects of purified anti-PDI antibodies on purified bovine PDI activity. Purified Bovine PDI activity was measured in the presence of 0–60 µg/ml purified anti- bovine PDI from anti-PDI positive subjects (triangles) and 0–160 µg/ml total IgG from rabbit immuned against bovine PDI (diamonds). Pooled human and rabbit IgG (dashed lines) were used as control. Results are compared to activity in the presence of same volume of PBS (defined as 100%) and presented as a mean ± SEM of three experiments. PDI activity was measured using the insulin transhydrogenase assay. ( B ) Effects of human sera (1∶50) on human recombinant PDI activity. Recombinant human PDI activity was measured in the presence of 20 µl/ml human sera positive or negative for anti-human PDI antibody. Results are presented as mean ± SEM. ( C ) Association of anti- human PDI status with serum insulin levels. Results are presented as mean ± SEM, adjusted for age, gender, education, BMI and smoking. ( D ) Association of anti-human PDI status with insulin resistance. Insulin resistance was estimated using HOMA (Homeostatic Model Assessment of insulin resistance). Results are presented as mean ± SEM, adjusted for age, gender, education, BMI and smoking. For details, see Table S2 .

    Article Snippet: Antibodies: Rabbit polyclonal anti-bovine PDI, human IgG, and rabbit IgG from Sigma-Aldrich; Donkey anti-human IgG conjugated with horseradish peroxidase - from Jackson ImmunoResearch Laboratories.

    Techniques: Functional Assay, Activity Assay, Purification, Recombinant

    PDI is a target of post-streptococcal autoimmunity. Proteins of various sources (described below) were separated by SDS-PAGE, transferred to membranes, and probed with serum, total IgG, or affinity-purified antibodies extracted from participants. (A) Association of ASLO status with auto-immunoreactivity, as detected by a 58 KDa immunoreactive band. Rat urinary bladder extracts (15 µg/strip) were probed with selected ASLO positive (≥400IU; lanes 1–4, 6 and 8) and negative (

    Journal: PLoS ONE

    Article Title: Post-Streptococcal Auto-Antibodies Inhibit Protein Disulfide Isomerase and Are Associated with Insulin Resistance

    doi: 10.1371/journal.pone.0012875

    Figure Lengend Snippet: PDI is a target of post-streptococcal autoimmunity. Proteins of various sources (described below) were separated by SDS-PAGE, transferred to membranes, and probed with serum, total IgG, or affinity-purified antibodies extracted from participants. (A) Association of ASLO status with auto-immunoreactivity, as detected by a 58 KDa immunoreactive band. Rat urinary bladder extracts (15 µg/strip) were probed with selected ASLO positive (≥400IU; lanes 1–4, 6 and 8) and negative (

    Article Snippet: Antibodies: Rabbit polyclonal anti-bovine PDI, human IgG, and rabbit IgG from Sigma-Aldrich; Donkey anti-human IgG conjugated with horseradish peroxidase - from Jackson ImmunoResearch Laboratories.

    Techniques: SDS Page, Affinity Purification, Stripping Membranes