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    Alomone Labs guinea pig anti scn11a nav1 9 antibody
    <t>Nav1.9</t> knockout affects ribbon synapse density and survival of spiral ganglion neurons. Representative images of ribbon synapse immunostained with Ctbp2 (green) from WT ( a ) and Nav1.9 −/− mice ( b ). c Quantitative analysis of ribbon synapse counts per IHC from five randomly selected visual fields for each mouse. n = 7 for WT group, n = 5 for Nav1.9 −/− group. * p = 0.034 by Mann–Whitney test. Representative images of spiral ganglion neurons in Rosenthal’s canal from WT ( d ) and Nav1.9 −/− mice ( e ). f Spiral ganglion neuron counts in the basal turn, the middle turn and apical turn together in 3 midmodiolar sections for each animal. n = 5 for WT group, n = 4 for Nav1.9 −/− group. * p = 0.014 by Mann–Whitney test. Data are expressed as mean ± s.d
    Guinea Pig Anti Scn11a Nav1 9 Antibody, supplied by Alomone Labs, used in various techniques. Bioz Stars score: 93/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    Nav1.9 knockout affects ribbon synapse density and survival of spiral ganglion neurons. Representative images of ribbon synapse immunostained with Ctbp2 (green) from WT ( a ) and Nav1.9 −/− mice ( b ). c Quantitative analysis of ribbon synapse counts per IHC from five randomly selected visual fields for each mouse. n = 7 for WT group, n = 5 for Nav1.9 −/− group. * p = 0.034 by Mann–Whitney test. Representative images of spiral ganglion neurons in Rosenthal’s canal from WT ( d ) and Nav1.9 −/− mice ( e ). f Spiral ganglion neuron counts in the basal turn, the middle turn and apical turn together in 3 midmodiolar sections for each animal. n = 5 for WT group, n = 4 for Nav1.9 −/− group. * p = 0.014 by Mann–Whitney test. Data are expressed as mean ± s.d

    Journal: BMC Neuroscience

    Article Title: SCN11A gene deletion causes sensorineural hearing loss by impairing the ribbon synapses and auditory nerves

    doi: 10.1186/s12868-021-00613-8

    Figure Lengend Snippet: Nav1.9 knockout affects ribbon synapse density and survival of spiral ganglion neurons. Representative images of ribbon synapse immunostained with Ctbp2 (green) from WT ( a ) and Nav1.9 −/− mice ( b ). c Quantitative analysis of ribbon synapse counts per IHC from five randomly selected visual fields for each mouse. n = 7 for WT group, n = 5 for Nav1.9 −/− group. * p = 0.034 by Mann–Whitney test. Representative images of spiral ganglion neurons in Rosenthal’s canal from WT ( d ) and Nav1.9 −/− mice ( e ). f Spiral ganglion neuron counts in the basal turn, the middle turn and apical turn together in 3 midmodiolar sections for each animal. n = 5 for WT group, n = 4 for Nav1.9 −/− group. * p = 0.014 by Mann–Whitney test. Data are expressed as mean ± s.d

    Article Snippet: Immunohistochemistry and synaptic counts After washes with 0.1% Triton X-100 in PBS, sections on adhesion microscope slides were blocked with 10% normal goat serum (ZLI-9021, ZSGB-BIO) and incubated with rabbit anti-SCN11A polyclonal antibody (AT322395, 1:200, OriGene, Rockville, MD), guinea pig anti-Nav1.9 polyclonal antibody (AGP-030, 1:200, alomone labs, Israel), mouse anti-CtBP2 (612044, 1:100, BD Biosciences), in 10% goat serum diluted in 0.1 M PBS at 4 ℃ overnight and then incubated with secondary antibodies containing anti-mouse Alexa Fluo™ 488 (lot 1810918, 1:400, goat, Thermo Fisher), anti-rabbit Alexa Fluor™ 568 (lot 1494753, 1:400, goat, Thermo Fisher), or anti-guinea pig Alexa FluroTM647 (A-21450, 1:400, goat, Thermo Fisher).

    Techniques: Knock-Out, Mouse Assay, Immunohistochemistry, MANN-WHITNEY

    Nav1.9 knockout does not affect the morphology of hair cells. a The digital image of a dissected cochlea including the hook region (left) from a 4 months old mouse. Schematic drawing of the same cochlea with percent distance from the apex plotted (right). b Scale is showing frequency, percent distance from the apex, and distance (mm), according to Müller et al. [ 22 ]: x = 100 − (156.53 − 82.46 * log(F)). The full basilar membrane length is 6.3 mm for this particular cochlea. C, Images of organ of Cortis from 4 months old mice stained by DAPI (blue), with the apical turn (0–25% distance from the apex), the middle turn (30–55% distance from the apex) and the basal turn (60–85% distance from the apex). d Images of the organ of Corti of Nav1.9 −/− mice at postnatal ages of 2 months by SEM, containing the apical turn ( b1 – b3 ), the middle turn ( b4 – b6 ), and the basal turn ( b7 – b9 )

    Journal: BMC Neuroscience

    Article Title: SCN11A gene deletion causes sensorineural hearing loss by impairing the ribbon synapses and auditory nerves

    doi: 10.1186/s12868-021-00613-8

    Figure Lengend Snippet: Nav1.9 knockout does not affect the morphology of hair cells. a The digital image of a dissected cochlea including the hook region (left) from a 4 months old mouse. Schematic drawing of the same cochlea with percent distance from the apex plotted (right). b Scale is showing frequency, percent distance from the apex, and distance (mm), according to Müller et al. [ 22 ]: x = 100 − (156.53 − 82.46 * log(F)). The full basilar membrane length is 6.3 mm for this particular cochlea. C, Images of organ of Cortis from 4 months old mice stained by DAPI (blue), with the apical turn (0–25% distance from the apex), the middle turn (30–55% distance from the apex) and the basal turn (60–85% distance from the apex). d Images of the organ of Corti of Nav1.9 −/− mice at postnatal ages of 2 months by SEM, containing the apical turn ( b1 – b3 ), the middle turn ( b4 – b6 ), and the basal turn ( b7 – b9 )

    Article Snippet: Immunohistochemistry and synaptic counts After washes with 0.1% Triton X-100 in PBS, sections on adhesion microscope slides were blocked with 10% normal goat serum (ZLI-9021, ZSGB-BIO) and incubated with rabbit anti-SCN11A polyclonal antibody (AT322395, 1:200, OriGene, Rockville, MD), guinea pig anti-Nav1.9 polyclonal antibody (AGP-030, 1:200, alomone labs, Israel), mouse anti-CtBP2 (612044, 1:100, BD Biosciences), in 10% goat serum diluted in 0.1 M PBS at 4 ℃ overnight and then incubated with secondary antibodies containing anti-mouse Alexa Fluo™ 488 (lot 1810918, 1:400, goat, Thermo Fisher), anti-rabbit Alexa Fluor™ 568 (lot 1494753, 1:400, goat, Thermo Fisher), or anti-guinea pig Alexa FluroTM647 (A-21450, 1:400, goat, Thermo Fisher).

    Techniques: Knock-Out, Mouse Assay, Staining

    CRISPR/Cas 9-mediated generation of a Nav1.9 −/− mouse model. a A representative illustration of the CRISPR/Cas9 targeting strategy for generating Nav1.9 knockout (KO) mice. The Cas9 mRNA and two single guide RNAs targeting a region from SCN11A exon 3 to 5, were microinjected into mouse zygotes. b Schematic diagram of primer pair design for PCR genotyping, a representative PCR genotyping result for Nav1.9 wild-type (WT), homozygous (Nav1.9 −/− ) and heterozygous (Nav1.9 +/− ), the region of junction of DSB is absent in the WT mice. Primer 2: primer pairs containing forward primer and KO specific reverse primer; Primer 1: primer pairs containing forward primer and WT specific reverse primer. c This successfully eliminated all of exon 3, 4 and 5, as confirmed by Sanger sequencing, induces reading frame shift and thus a premature translational- termination codon during the truncated protein expression. d The protein expression of Nav1.9 in the cochleas of Nav1.9 −/− mice (n = 3) or WT mice (n = 4) was measured by western blot

    Journal: BMC Neuroscience

    Article Title: SCN11A gene deletion causes sensorineural hearing loss by impairing the ribbon synapses and auditory nerves

    doi: 10.1186/s12868-021-00613-8

    Figure Lengend Snippet: CRISPR/Cas 9-mediated generation of a Nav1.9 −/− mouse model. a A representative illustration of the CRISPR/Cas9 targeting strategy for generating Nav1.9 knockout (KO) mice. The Cas9 mRNA and two single guide RNAs targeting a region from SCN11A exon 3 to 5, were microinjected into mouse zygotes. b Schematic diagram of primer pair design for PCR genotyping, a representative PCR genotyping result for Nav1.9 wild-type (WT), homozygous (Nav1.9 −/− ) and heterozygous (Nav1.9 +/− ), the region of junction of DSB is absent in the WT mice. Primer 2: primer pairs containing forward primer and KO specific reverse primer; Primer 1: primer pairs containing forward primer and WT specific reverse primer. c This successfully eliminated all of exon 3, 4 and 5, as confirmed by Sanger sequencing, induces reading frame shift and thus a premature translational- termination codon during the truncated protein expression. d The protein expression of Nav1.9 in the cochleas of Nav1.9 −/− mice (n = 3) or WT mice (n = 4) was measured by western blot

    Article Snippet: Immunohistochemistry and synaptic counts After washes with 0.1% Triton X-100 in PBS, sections on adhesion microscope slides were blocked with 10% normal goat serum (ZLI-9021, ZSGB-BIO) and incubated with rabbit anti-SCN11A polyclonal antibody (AT322395, 1:200, OriGene, Rockville, MD), guinea pig anti-Nav1.9 polyclonal antibody (AGP-030, 1:200, alomone labs, Israel), mouse anti-CtBP2 (612044, 1:100, BD Biosciences), in 10% goat serum diluted in 0.1 M PBS at 4 ℃ overnight and then incubated with secondary antibodies containing anti-mouse Alexa Fluo™ 488 (lot 1810918, 1:400, goat, Thermo Fisher), anti-rabbit Alexa Fluor™ 568 (lot 1494753, 1:400, goat, Thermo Fisher), or anti-guinea pig Alexa FluroTM647 (A-21450, 1:400, goat, Thermo Fisher).

    Techniques: CRISPR, Knock-Out, Mouse Assay, Polymerase Chain Reaction, Sequencing, Expressing, Western Blot

    Distribution of Nav1.9 in primary auditory afferents. a The voltage-gated sodium channel Nav1.9 and Nav1.1 mRNA levels in modiolus of WT ICR mice at the postnatal 0, 7th, 14th, 21th, 28th and 60th day. Each time point contains 5 mice. * p = 0.028, ** p = 0.004. b A schematic representing the localization of Nav1.9 channels at primary afferent peripheral nerve endings on hair cells in cochlea, in SGN somata, in the auditory nerve located within the modiolus, and in the cochlear nuclei. c Nav1.9 is present in cochlea basilar membrane by surface preparation technique and immunofluorescence staining in cryo-section. c1 Horizontal section showing three rows of OHCs and one row of IHCs. In a linear distribution below the IHCs, Nav1.9 (purple) is in the afferent endings beneath the IHC bases. Also stained are the afferent radial fibers leading through the tunnel of Corti to their first hemi-nodes beneath the foramina nervosa. Scales = 75 μm. c2 The diagram of the cochlea’s afferent innervations pattern. c3 Nav1.9 is in the nerve endings of internal spiral fibers or radial fibers beneath IHC (red), the cilia of which exhibit phalloidin labeling (green). Scales = 50 μm. c4 The high magnification image of c3. Scale = 10 μm. d The expression of Nav1.9 in the SGNs of P60 WT mouse was measured by immunofluorescence. Nav1.9, MBP, and cell nucleus are stained as red, green and blue, respectively. e Some neurons from the dorsal cochlear nucleus are labeled by Nav1.9 (red)

    Journal: BMC Neuroscience

    Article Title: SCN11A gene deletion causes sensorineural hearing loss by impairing the ribbon synapses and auditory nerves

    doi: 10.1186/s12868-021-00613-8

    Figure Lengend Snippet: Distribution of Nav1.9 in primary auditory afferents. a The voltage-gated sodium channel Nav1.9 and Nav1.1 mRNA levels in modiolus of WT ICR mice at the postnatal 0, 7th, 14th, 21th, 28th and 60th day. Each time point contains 5 mice. * p = 0.028, ** p = 0.004. b A schematic representing the localization of Nav1.9 channels at primary afferent peripheral nerve endings on hair cells in cochlea, in SGN somata, in the auditory nerve located within the modiolus, and in the cochlear nuclei. c Nav1.9 is present in cochlea basilar membrane by surface preparation technique and immunofluorescence staining in cryo-section. c1 Horizontal section showing three rows of OHCs and one row of IHCs. In a linear distribution below the IHCs, Nav1.9 (purple) is in the afferent endings beneath the IHC bases. Also stained are the afferent radial fibers leading through the tunnel of Corti to their first hemi-nodes beneath the foramina nervosa. Scales = 75 μm. c2 The diagram of the cochlea’s afferent innervations pattern. c3 Nav1.9 is in the nerve endings of internal spiral fibers or radial fibers beneath IHC (red), the cilia of which exhibit phalloidin labeling (green). Scales = 50 μm. c4 The high magnification image of c3. Scale = 10 μm. d The expression of Nav1.9 in the SGNs of P60 WT mouse was measured by immunofluorescence. Nav1.9, MBP, and cell nucleus are stained as red, green and blue, respectively. e Some neurons from the dorsal cochlear nucleus are labeled by Nav1.9 (red)

    Article Snippet: Immunohistochemistry and synaptic counts After washes with 0.1% Triton X-100 in PBS, sections on adhesion microscope slides were blocked with 10% normal goat serum (ZLI-9021, ZSGB-BIO) and incubated with rabbit anti-SCN11A polyclonal antibody (AT322395, 1:200, OriGene, Rockville, MD), guinea pig anti-Nav1.9 polyclonal antibody (AGP-030, 1:200, alomone labs, Israel), mouse anti-CtBP2 (612044, 1:100, BD Biosciences), in 10% goat serum diluted in 0.1 M PBS at 4 ℃ overnight and then incubated with secondary antibodies containing anti-mouse Alexa Fluo™ 488 (lot 1810918, 1:400, goat, Thermo Fisher), anti-rabbit Alexa Fluor™ 568 (lot 1494753, 1:400, goat, Thermo Fisher), or anti-guinea pig Alexa FluroTM647 (A-21450, 1:400, goat, Thermo Fisher).

    Techniques: Mouse Assay, Immunofluorescence, Staining, Immunohistochemistry, Labeling, Expressing

    Auditory compound action potentials are affected by Nav1.9 knockout. Nav1.9 knockout induces higher CAP P1 threshold, * p = 0.013 with Mann–Whitney test ( a ), lower CAP P1 amplitude, * p = 0.041 with independent samples t test ( b ), compared with WT group; c the CAP P1 latency is not affected at the time point of postnatal day 60, p = 0.242 with independent samples t test. d Representative CAP waveforms from a WT mouse. e Representative CAP waveforms from a Nav1.9 −/− mice. Data are expressed as mean ± s.d

    Journal: BMC Neuroscience

    Article Title: SCN11A gene deletion causes sensorineural hearing loss by impairing the ribbon synapses and auditory nerves

    doi: 10.1186/s12868-021-00613-8

    Figure Lengend Snippet: Auditory compound action potentials are affected by Nav1.9 knockout. Nav1.9 knockout induces higher CAP P1 threshold, * p = 0.013 with Mann–Whitney test ( a ), lower CAP P1 amplitude, * p = 0.041 with independent samples t test ( b ), compared with WT group; c the CAP P1 latency is not affected at the time point of postnatal day 60, p = 0.242 with independent samples t test. d Representative CAP waveforms from a WT mouse. e Representative CAP waveforms from a Nav1.9 −/− mice. Data are expressed as mean ± s.d

    Article Snippet: Immunohistochemistry and synaptic counts After washes with 0.1% Triton X-100 in PBS, sections on adhesion microscope slides were blocked with 10% normal goat serum (ZLI-9021, ZSGB-BIO) and incubated with rabbit anti-SCN11A polyclonal antibody (AT322395, 1:200, OriGene, Rockville, MD), guinea pig anti-Nav1.9 polyclonal antibody (AGP-030, 1:200, alomone labs, Israel), mouse anti-CtBP2 (612044, 1:100, BD Biosciences), in 10% goat serum diluted in 0.1 M PBS at 4 ℃ overnight and then incubated with secondary antibodies containing anti-mouse Alexa Fluo™ 488 (lot 1810918, 1:400, goat, Thermo Fisher), anti-rabbit Alexa Fluor™ 568 (lot 1494753, 1:400, goat, Thermo Fisher), or anti-guinea pig Alexa FluroTM647 (A-21450, 1:400, goat, Thermo Fisher).

    Techniques: Knock-Out, MANN-WHITNEY, Mouse Assay

    Audiological characterization of Nav1.9 −/− mice. a Mean ABR thresholds of six wild-type, four heterozygous and seven homozygous versus sound frequency, ** p = 0.002, ** p = 0.001 at 12 kHz compared with homozygous, * p = 0.01, *** p = 0.000 at 16 kHz compared with homozygous by one-way ANOVA with Bonferroni’s post-hoc test. b Example of ABR waveforms at 16 kHz in one ear of a wild-type superimposed on an example of ABR waveforms in one ear of Nav1.9 −/− mice. c ABR thresholds of WT and homozygous mice of postnatal day 21 to 60 at 8 kHz. p = 0.807 at P21; p = 0.932 at P30; p = 0.504 with independent samples t test; n.s.: not significant. d ABR threshold of WT and homozygous mice of postnatal day 21 to 60 at 16 kHz. * p = 0.016, ** p = 0.006, *** p = 0.000 with Mann–Whitney test. Data are expressed as mean ± s.d

    Journal: BMC Neuroscience

    Article Title: SCN11A gene deletion causes sensorineural hearing loss by impairing the ribbon synapses and auditory nerves

    doi: 10.1186/s12868-021-00613-8

    Figure Lengend Snippet: Audiological characterization of Nav1.9 −/− mice. a Mean ABR thresholds of six wild-type, four heterozygous and seven homozygous versus sound frequency, ** p = 0.002, ** p = 0.001 at 12 kHz compared with homozygous, * p = 0.01, *** p = 0.000 at 16 kHz compared with homozygous by one-way ANOVA with Bonferroni’s post-hoc test. b Example of ABR waveforms at 16 kHz in one ear of a wild-type superimposed on an example of ABR waveforms in one ear of Nav1.9 −/− mice. c ABR thresholds of WT and homozygous mice of postnatal day 21 to 60 at 8 kHz. p = 0.807 at P21; p = 0.932 at P30; p = 0.504 with independent samples t test; n.s.: not significant. d ABR threshold of WT and homozygous mice of postnatal day 21 to 60 at 16 kHz. * p = 0.016, ** p = 0.006, *** p = 0.000 with Mann–Whitney test. Data are expressed as mean ± s.d

    Article Snippet: Immunohistochemistry and synaptic counts After washes with 0.1% Triton X-100 in PBS, sections on adhesion microscope slides were blocked with 10% normal goat serum (ZLI-9021, ZSGB-BIO) and incubated with rabbit anti-SCN11A polyclonal antibody (AT322395, 1:200, OriGene, Rockville, MD), guinea pig anti-Nav1.9 polyclonal antibody (AGP-030, 1:200, alomone labs, Israel), mouse anti-CtBP2 (612044, 1:100, BD Biosciences), in 10% goat serum diluted in 0.1 M PBS at 4 ℃ overnight and then incubated with secondary antibodies containing anti-mouse Alexa Fluo™ 488 (lot 1810918, 1:400, goat, Thermo Fisher), anti-rabbit Alexa Fluor™ 568 (lot 1494753, 1:400, goat, Thermo Fisher), or anti-guinea pig Alexa FluroTM647 (A-21450, 1:400, goat, Thermo Fisher).

    Techniques: Mouse Assay, MANN-WHITNEY