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  • 94
    Millipore gdp
    Gdp, supplied by Millipore, used in various techniques. Bioz Stars score: 94/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/gdp/product/Millipore
    Average 94 stars, based on 1 article reviews
    Price from $9.99 to $1999.99
    gdp - by Bioz Stars, 2021-09
    94/100 stars
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    93
    Jena Bioscience gmpcpp
    FCF does not affect the thermostability of SEPT2 dimers in the presence of GDP, but increases SEPT2 stability in the presence of <t>GmpCpp.</t> Recombinant His-tagged SEPT2 dimers were purified using size exclusion chromatography and SEPT2 dimer stability was assayed by differential scanning fluorimetry. (A and B) Curves show the negative first derivative of SYPRO ORANGE fluorescence plotted against temperature for SEPT2 dimers in the presence of <t>DMSO</t> (control), FCF (100 µM), GDP (100 µM), and FCF plus GDP (100 µM each). Plot (B) shows the median melting temperature of SEPT2 from three independent experiments. Error bars represent the highest and lowest values obtained from these experiments. (C and D) Curves show the negative first derivative of SYPRO ORANGE fluorescence plotted against temperature for SEPT2 dimers in the presence of DMSO (control), FCF (100 µM), GmpCpp (100 µM), and FCF plus CmpCpp (100 µM each). Plot (D) shows the median melting temperature of SEPT2 from three independent experiments. Error bars represent the highest and lowest values obtained from these independent experiments.
    Gmpcpp, supplied by Jena Bioscience, used in various techniques. Bioz Stars score: 93/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/gmpcpp/product/Jena Bioscience
    Average 93 stars, based on 1 article reviews
    Price from $9.99 to $1999.99
    gmpcpp - by Bioz Stars, 2021-09
    93/100 stars
      Buy from Supplier

    94
    Millipore guanosine 5
    FCF does not affect the thermostability of SEPT2 dimers in the presence of GDP, but increases SEPT2 stability in the presence of <t>GmpCpp.</t> Recombinant His-tagged SEPT2 dimers were purified using size exclusion chromatography and SEPT2 dimer stability was assayed by differential scanning fluorimetry. (A and B) Curves show the negative first derivative of SYPRO ORANGE fluorescence plotted against temperature for SEPT2 dimers in the presence of <t>DMSO</t> (control), FCF (100 µM), GDP (100 µM), and FCF plus GDP (100 µM each). Plot (B) shows the median melting temperature of SEPT2 from three independent experiments. Error bars represent the highest and lowest values obtained from these experiments. (C and D) Curves show the negative first derivative of SYPRO ORANGE fluorescence plotted against temperature for SEPT2 dimers in the presence of DMSO (control), FCF (100 µM), GmpCpp (100 µM), and FCF plus CmpCpp (100 µM each). Plot (D) shows the median melting temperature of SEPT2 from three independent experiments. Error bars represent the highest and lowest values obtained from these independent experiments.
    Guanosine 5, supplied by Millipore, used in various techniques. Bioz Stars score: 94/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/guanosine 5/product/Millipore
    Average 94 stars, based on 1 article reviews
    Price from $9.99 to $1999.99
    guanosine 5 - by Bioz Stars, 2021-09
    94/100 stars
      Buy from Supplier

    97
    Millipore guanosine 5 o
    FCF does not affect the thermostability of SEPT2 dimers in the presence of GDP, but increases SEPT2 stability in the presence of <t>GmpCpp.</t> Recombinant His-tagged SEPT2 dimers were purified using size exclusion chromatography and SEPT2 dimer stability was assayed by differential scanning fluorimetry. (A and B) Curves show the negative first derivative of SYPRO ORANGE fluorescence plotted against temperature for SEPT2 dimers in the presence of <t>DMSO</t> (control), FCF (100 µM), GDP (100 µM), and FCF plus GDP (100 µM each). Plot (B) shows the median melting temperature of SEPT2 from three independent experiments. Error bars represent the highest and lowest values obtained from these experiments. (C and D) Curves show the negative first derivative of SYPRO ORANGE fluorescence plotted against temperature for SEPT2 dimers in the presence of DMSO (control), FCF (100 µM), GmpCpp (100 µM), and FCF plus CmpCpp (100 µM each). Plot (D) shows the median melting temperature of SEPT2 from three independent experiments. Error bars represent the highest and lowest values obtained from these independent experiments.
    Guanosine 5 O, supplied by Millipore, used in various techniques. Bioz Stars score: 97/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/guanosine 5 o/product/Millipore
    Average 97 stars, based on 1 article reviews
    Price from $9.99 to $1999.99
    guanosine 5 o - by Bioz Stars, 2021-09
    97/100 stars
      Buy from Supplier

    Image Search Results


    FCF does not affect the thermostability of SEPT2 dimers in the presence of GDP, but increases SEPT2 stability in the presence of GmpCpp. Recombinant His-tagged SEPT2 dimers were purified using size exclusion chromatography and SEPT2 dimer stability was assayed by differential scanning fluorimetry. (A and B) Curves show the negative first derivative of SYPRO ORANGE fluorescence plotted against temperature for SEPT2 dimers in the presence of DMSO (control), FCF (100 µM), GDP (100 µM), and FCF plus GDP (100 µM each). Plot (B) shows the median melting temperature of SEPT2 from three independent experiments. Error bars represent the highest and lowest values obtained from these experiments. (C and D) Curves show the negative first derivative of SYPRO ORANGE fluorescence plotted against temperature for SEPT2 dimers in the presence of DMSO (control), FCF (100 µM), GmpCpp (100 µM), and FCF plus CmpCpp (100 µM each). Plot (D) shows the median melting temperature of SEPT2 from three independent experiments. Error bars represent the highest and lowest values obtained from these independent experiments.

    Journal: PLoS ONE

    Article Title: In Silico Docking of Forchlorfenuron (FCF) to Septins Suggests that FCF Interferes with GTP Binding

    doi: 10.1371/journal.pone.0096390

    Figure Lengend Snippet: FCF does not affect the thermostability of SEPT2 dimers in the presence of GDP, but increases SEPT2 stability in the presence of GmpCpp. Recombinant His-tagged SEPT2 dimers were purified using size exclusion chromatography and SEPT2 dimer stability was assayed by differential scanning fluorimetry. (A and B) Curves show the negative first derivative of SYPRO ORANGE fluorescence plotted against temperature for SEPT2 dimers in the presence of DMSO (control), FCF (100 µM), GDP (100 µM), and FCF plus GDP (100 µM each). Plot (B) shows the median melting temperature of SEPT2 from three independent experiments. Error bars represent the highest and lowest values obtained from these experiments. (C and D) Curves show the negative first derivative of SYPRO ORANGE fluorescence plotted against temperature for SEPT2 dimers in the presence of DMSO (control), FCF (100 µM), GmpCpp (100 µM), and FCF plus CmpCpp (100 µM each). Plot (D) shows the median melting temperature of SEPT2 from three independent experiments. Error bars represent the highest and lowest values obtained from these independent experiments.

    Article Snippet: SYPRO Orange (Invitrogen) was added at a final concentration of 5× (1∶1,000 dilution) into 50 µl of reaction buffer (50 mM Tris pH 8.0, 50 mM NaCl) containing purified recombinant His-SEPT2 in the presence of DMSO (Sigma-Aldrich), FCF (Sigma-Aldrich), GDP (guanosine 5′-diphosphate; Sigma-Aldrich) or GmpCpp (guanosine-5′-[(a,β)-methylenotriphosphate; Jena Bioscience).

    Techniques: Recombinant, Purification, Size-exclusion Chromatography, Fluorescence