Journal: Chemical Science
Article Title: pH- and ligand-induced release of loads from DNA–acrylamide hydrogel microcapsules and ligand-induced release of loads from DNA–acrylamide hydrogel microcapsules †Electronic supplementary information (ESI) available: Nucleic acid sequences; characterization of nucleic acid-modified copolymers; SEM images, confocal images of microcapsules; calibration curves of TMR-D, TR-D, and DOX-D; synthesis of DOX-D; CD spectra of pH-responsive hydrogel; characterization of hydrogel membranes. See DOI: 10.1039/c6sc04770jClick here for additional data file.
Figure Lengend Snippet: (A) Time-dependent release of DOX-D from the ATP-responsive hydrogel microcapsules treated with 50 mM ATP (a) and without added ATP (b). The released DOX-D was acidified to enable the cleavage of fluorescent doxorubicin from dextran. (B) Fluorescence spectra corresponding to the release of doxorubicin upon exposing the ATP-responsive hydrogel microcapsules to different concentrations of ATP for a fixed time interval of 30 min: (a) 0, (b) 5, (c) 10, (d) 20, (e) 40, (f) 50, and (g) 75 mM. Inset: derived calibration curve of the fluorescence intensities of the released doxorubicin at λ em = 600 nm upon treatment with different concentrations of ATP. (C) Fluorescence spectra corresponding to the release of doxorubicin from the ATP-responsive microcapsules loaded with doxorubicin-dextran and exposed to different nucleoside triphosphates for a fixed time interval of 30 minutes: (a) 25 mM ATP, (b) 25 mM CTP, (c) 25 mM GTP, and (d) untreated.
Article Snippet: Reagents and materials Magnesium chloride, sodium chloride, doxorubicin hydrochloride (DOX), 4-carboxyphenylboronic acid, dextran (MW = 40 kDa), 4-(2-hydroxyethyl)piperazine-1-ethanesulfonic acid sodium salt (HEPES base), 4-(2-hydroxyethyl)piperazine-1-ethanesulfonic acid (HEPES acid), N -(3-dimethylaminopropyl)-N ′-ethylcarbodiimide hydrochloride (EDC), N -hydroxysulfosuccinimide sodium salt (sulfo-NHS), poly(acrylic acid) (MW = 30, 450, and 1250 kDa), 2-(N -morpholino)ethanesulfonic acid (MES), ammonium persulfate (APS), N ,N ,N ′,N ′-tetramethylethylenediamine (TEMED), acrylamide solution (40%), poly(allylamine hydrochloride) (PAH, MW = 58 kDa), ethylenediaminetetraacetic acid disodium salt dihydrate (EDTA), adenosine triphosphate (ATP), guanosine triphosphate (GTP), cytidine triphosphate (CTP), and thymidine triphosphate (TTP) were purchased from Sigma-Aldrich.
Techniques: Fluorescence, Derivative Assay