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  • 99
    Promega gotaq dna polymerase
    Optimization of cloning conditions . (A) PCR amplification of a target cDNA (human nAChR α9 subunit) and the pGEMHE vector using different <t>DNA</t> polymerases: Pfu Turbo, PfuUltra and Phusion. (B) Comparison of number of colonies grown on the plates after transformation. Three different vector-to-insert ratios (1:1, 1:2, and 1:4) during Dpn I digestion and three amounts of vector-insert mixtures (2, 4, and 8 μl) for transformation were tested. See text for details. (C) Clone validation by PCR using <t>GoTaq</t> DNA polymerase. Lanes 1 to 12: target clones to be validated; Lane 13: 1 Kb plus DNA ladder; Lane 14: pGEMHE vector control; Lane 15: negative control using pCR4-TOPO-α9 parent plasmid. (D) Clone validation by restriction digestion to exclude unusual constructs. Lane 1: 1 Kb plus DNA ladder, Lanes 2-11: target clones double digested with Kpn I and Nhe I. Note that this digestion resulted in a pGEM vector and an insert with α9 nAChR plus the 5'UTR and 3'UTR of Xenopus β-globin.
    Gotaq Dna Polymerase, supplied by Promega, used in various techniques. Bioz Stars score: 99/100, based on 13947 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/gotaq dna polymerase/product/Promega
    Average 99 stars, based on 13947 article reviews
    Price from $9.99 to $1999.99
    gotaq dna polymerase - by Bioz Stars, 2020-08
    99/100 stars
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    90
    Promega gotaq g2 dna polymerase
    Optimization of cloning conditions . (A) PCR amplification of a target cDNA (human nAChR α9 subunit) and the pGEMHE vector using different <t>DNA</t> polymerases: Pfu Turbo, PfuUltra and Phusion. (B) Comparison of number of colonies grown on the plates after transformation. Three different vector-to-insert ratios (1:1, 1:2, and 1:4) during Dpn I digestion and three amounts of vector-insert mixtures (2, 4, and 8 μl) for transformation were tested. See text for details. (C) Clone validation by PCR using <t>GoTaq</t> DNA polymerase. Lanes 1 to 12: target clones to be validated; Lane 13: 1 Kb plus DNA ladder; Lane 14: pGEMHE vector control; Lane 15: negative control using pCR4-TOPO-α9 parent plasmid. (D) Clone validation by restriction digestion to exclude unusual constructs. Lane 1: 1 Kb plus DNA ladder, Lanes 2-11: target clones double digested with Kpn I and Nhe I. Note that this digestion resulted in a pGEM vector and an insert with α9 nAChR plus the 5'UTR and 3'UTR of Xenopus β-globin.
    Gotaq G2 Dna Polymerase, supplied by Promega, used in various techniques. Bioz Stars score: 90/100, based on 507 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/gotaq g2 dna polymerase/product/Promega
    Average 90 stars, based on 507 article reviews
    Price from $9.99 to $1999.99
    gotaq g2 dna polymerase - by Bioz Stars, 2020-08
    90/100 stars
      Buy from Supplier

    Image Search Results


    Optimization of cloning conditions . (A) PCR amplification of a target cDNA (human nAChR α9 subunit) and the pGEMHE vector using different DNA polymerases: Pfu Turbo, PfuUltra and Phusion. (B) Comparison of number of colonies grown on the plates after transformation. Three different vector-to-insert ratios (1:1, 1:2, and 1:4) during Dpn I digestion and three amounts of vector-insert mixtures (2, 4, and 8 μl) for transformation were tested. See text for details. (C) Clone validation by PCR using GoTaq DNA polymerase. Lanes 1 to 12: target clones to be validated; Lane 13: 1 Kb plus DNA ladder; Lane 14: pGEMHE vector control; Lane 15: negative control using pCR4-TOPO-α9 parent plasmid. (D) Clone validation by restriction digestion to exclude unusual constructs. Lane 1: 1 Kb plus DNA ladder, Lanes 2-11: target clones double digested with Kpn I and Nhe I. Note that this digestion resulted in a pGEM vector and an insert with α9 nAChR plus the 5'UTR and 3'UTR of Xenopus β-globin.

    Journal: BMC Biotechnology

    Article Title: FastCloning: a highly simplified, purification-free, sequence- and ligation-independent PCR cloning method

    doi: 10.1186/1472-6750-11-92

    Figure Lengend Snippet: Optimization of cloning conditions . (A) PCR amplification of a target cDNA (human nAChR α9 subunit) and the pGEMHE vector using different DNA polymerases: Pfu Turbo, PfuUltra and Phusion. (B) Comparison of number of colonies grown on the plates after transformation. Three different vector-to-insert ratios (1:1, 1:2, and 1:4) during Dpn I digestion and three amounts of vector-insert mixtures (2, 4, and 8 μl) for transformation were tested. See text for details. (C) Clone validation by PCR using GoTaq DNA polymerase. Lanes 1 to 12: target clones to be validated; Lane 13: 1 Kb plus DNA ladder; Lane 14: pGEMHE vector control; Lane 15: negative control using pCR4-TOPO-α9 parent plasmid. (D) Clone validation by restriction digestion to exclude unusual constructs. Lane 1: 1 Kb plus DNA ladder, Lanes 2-11: target clones double digested with Kpn I and Nhe I. Note that this digestion resulted in a pGEM vector and an insert with α9 nAChR plus the 5'UTR and 3'UTR of Xenopus β-globin.

    Article Snippet: The PCR confirmation of the insert in the target vector (Figure ) was performed with the GoTaq DNA polymerase (Promega Corporation, Madison, WI) using the pGEMHE vector specific primer pair.

    Techniques: Clone Assay, Polymerase Chain Reaction, Amplification, Plasmid Preparation, Transformation Assay, Negative Control, Construct

    Expression of At. ferrivorans CF27-specific genes. RT-PCR experiments were performed on RNA extracted from At. ferrivorans CF27 Fe(II)-grown cells (F) or sulfur attached cells (S) without reverse transcriptase (–), with reverse transcriptase (+), and on genomic DNA from At. ferrivorans CF27 (D). AFERRI_v2 (or v1) number of each gene is shown with the corresponding cluster number in square brackets. The size of the expected PCR products is indicated. M is the 1 Kbp plus DNA ladder from Invitrogen.

    Journal: Frontiers in Microbiology

    Article Title: Comparative Genome Analysis Provides Insights into Both the Lifestyle of Acidithiobacillus ferrivorans Strain CF27 and the Chimeric Nature of the Iron-Oxidizing Acidithiobacilli Genomes

    doi: 10.3389/fmicb.2017.01009

    Figure Lengend Snippet: Expression of At. ferrivorans CF27-specific genes. RT-PCR experiments were performed on RNA extracted from At. ferrivorans CF27 Fe(II)-grown cells (F) or sulfur attached cells (S) without reverse transcriptase (–), with reverse transcriptase (+), and on genomic DNA from At. ferrivorans CF27 (D). AFERRI_v2 (or v1) number of each gene is shown with the corresponding cluster number in square brackets. The size of the expected PCR products is indicated. M is the 1 Kbp plus DNA ladder from Invitrogen.

    Article Snippet: For each RT-PCR experiment, the hybridisation (55–66°C) and elongation (68 or 72°C) temperatures, RNA concentration (from 0.1 to 20 ng), DNA polymerase (GoTaq or Tfl from Promega), and the number of cycles (30, 35, or 40) were adjusted.

    Techniques: Expressing, Reverse Transcription Polymerase Chain Reaction, Polymerase Chain Reaction