goat anti-mouse igg2b cross-adsorbed secondary Search Results


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  • 99
    Thermo Fisher goat anti mouse igg h l cross adsorbed secondary antibody
    In vivo expression of non-tenocyte markers PPARγ, collagen type II, and osteocalcin in rats implanted with hTSCs treated with various concentrations of PGE 2 and their respective hematoxylin and eosin (H E) stained tissue sections. hTSCs cultured with three concentrations (0.1, 10, and 100 ng/ml) of PGE 2 were implanted subcutaneously into rats; later, immunohistochemical and histological analyses were performed on tissue sections. For the immunohistochemical staining, fixed tissue sections were incubated with mouse anti-human PPARγ antibody, mouse anti-collagen type II (Collagen II) antibody, or mouse anti-human osteocalcin antibody. Cy3-conjugated goat anti-mouse <t>IgG</t> was then used to detect primary binding. Nuclei were stained with Hoechst (blue). Expression levels of PPARγ, collagen type II, and osteocalcin (red) were lower in cells treated with 0.1 ng/ml PGE 2 ( A–C ) than those treated with the higher concentrations (10 and 100 ng/ml) of PGE 2 ( E–G, I–K ). H E staining was also performed on tissue sections ( D, H, L ). More cells (black dots; see insets in D , H , and L ) were observed in tissues implanted with hTSCs that had been treated with high concentrations of PGE 2 in culture ( H, L ). Specifically, at 100 ng/ml ( L ), cells were concentrated in a specific region (triangle). Additionally, semi-quantification of the stained cells was performed by counting immuno-positive cells and calculating percentage staining ( M ) (*p
    Goat Anti Mouse Igg H L Cross Adsorbed Secondary Antibody, supplied by Thermo Fisher, used in various techniques. Bioz Stars score: 99/100, based on 14081 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    91
    Thermo Fisher goat anti mouse igg1 cross adsorbed secondary antibody
    Immunoglobulin from healthy calves. (A) BAL sample; (B) blood serum. Means ± SEM from 10 calves during the 3 to 6 mo of life. *Statistical difference for <t>IgG1</t> BAL (T6 to T1, T2, and T3, P = 0.005).
    Goat Anti Mouse Igg1 Cross Adsorbed Secondary Antibody, supplied by Thermo Fisher, used in various techniques. Bioz Stars score: 91/100, based on 1246 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    95
    Thermo Fisher a21242
    Immunoglobulin from healthy calves. (A) BAL sample; (B) blood serum. Means ± SEM from 10 calves during the 3 to 6 mo of life. *Statistical difference for <t>IgG1</t> BAL (T6 to T1, T2, and T3, P = 0.005).
    A21242, supplied by Thermo Fisher, used in various techniques. Bioz Stars score: 95/100, based on 74 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    94
    Thermo Fisher goat anti rabbit igg h l highly cross adsorbed secondary antibody
    Immunoglobulin from healthy calves. (A) BAL sample; (B) blood serum. Means ± SEM from 10 calves during the 3 to 6 mo of life. *Statistical difference for <t>IgG1</t> BAL (T6 to T1, T2, and T3, P = 0.005).
    Goat Anti Rabbit Igg H L Highly Cross Adsorbed Secondary Antibody, supplied by Thermo Fisher, used in various techniques. Bioz Stars score: 94/100, based on 10173 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    Thermo Fisher goat anti mouse igg2b cross adsorbed secondary antibody hrp
    Immunoglobulin from healthy calves. (A) BAL sample; (B) blood serum. Means ± SEM from 10 calves during the 3 to 6 mo of life. *Statistical difference for <t>IgG1</t> BAL (T6 to T1, T2, and T3, P = 0.005).
    Goat Anti Mouse Igg2b Cross Adsorbed Secondary Antibody Hrp, supplied by Thermo Fisher, used in various techniques. Bioz Stars score: 93/100, based on 2 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    Thermo Fisher goat anti mouse igg2b secondary antibody
    Immunoglobulin from healthy calves. (A) BAL sample; (B) blood serum. Means ± SEM from 10 calves during the 3 to 6 mo of life. *Statistical difference for <t>IgG1</t> BAL (T6 to T1, T2, and T3, P = 0.005).
    Goat Anti Mouse Igg2b Secondary Antibody, supplied by Thermo Fisher, used in various techniques. Bioz Stars score: 99/100, based on 27 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    Thermo Fisher mouse anti goat igg
    Immunoglobulin from healthy calves. (A) BAL sample; (B) blood serum. Means ± SEM from 10 calves during the 3 to 6 mo of life. *Statistical difference for <t>IgG1</t> BAL (T6 to T1, T2, and T3, P = 0.005).
    Mouse Anti Goat Igg, supplied by Thermo Fisher, used in various techniques. Bioz Stars score: 93/100, based on 196 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    Thermo Fisher mouse igg3
    Immunoglobulin from healthy calves. (A) BAL sample; (B) blood serum. Means ± SEM from 10 calves during the 3 to 6 mo of life. *Statistical difference for <t>IgG1</t> BAL (T6 to T1, T2, and T3, P = 0.005).
    Mouse Igg3, supplied by Thermo Fisher, used in various techniques. Bioz Stars score: 94/100, based on 55 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    Thermo Fisher goat anti mouse igg h l highly cross adsorbed secondary antibody
    Immunoglobulin from healthy calves. (A) BAL sample; (B) blood serum. Means ± SEM from 10 calves during the 3 to 6 mo of life. *Statistical difference for <t>IgG1</t> BAL (T6 to T1, T2, and T3, P = 0.005).
    Goat Anti Mouse Igg H L Highly Cross Adsorbed Secondary Antibody, supplied by Thermo Fisher, used in various techniques. Bioz Stars score: 94/100, based on 9021 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    Thermo Fisher anti mouse igg
    Immunoglobulin from healthy calves. (A) BAL sample; (B) blood serum. Means ± SEM from 10 calves during the 3 to 6 mo of life. *Statistical difference for <t>IgG1</t> BAL (T6 to T1, T2, and T3, P = 0.005).
    Anti Mouse Igg, supplied by Thermo Fisher, used in various techniques. Bioz Stars score: 99/100, based on 6123 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    Thermo Fisher chicken anti goat igg h l cross adsorbed secondary antibody
    Immunoglobulin from healthy calves. (A) BAL sample; (B) blood serum. Means ± SEM from 10 calves during the 3 to 6 mo of life. *Statistical difference for <t>IgG1</t> BAL (T6 to T1, T2, and T3, P = 0.005).
    Chicken Anti Goat Igg H L Cross Adsorbed Secondary Antibody, supplied by Thermo Fisher, used in various techniques. Bioz Stars score: 99/100, based on 242 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    Image Search Results


    In vivo expression of non-tenocyte markers PPARγ, collagen type II, and osteocalcin in rats implanted with hTSCs treated with various concentrations of PGE 2 and their respective hematoxylin and eosin (H E) stained tissue sections. hTSCs cultured with three concentrations (0.1, 10, and 100 ng/ml) of PGE 2 were implanted subcutaneously into rats; later, immunohistochemical and histological analyses were performed on tissue sections. For the immunohistochemical staining, fixed tissue sections were incubated with mouse anti-human PPARγ antibody, mouse anti-collagen type II (Collagen II) antibody, or mouse anti-human osteocalcin antibody. Cy3-conjugated goat anti-mouse IgG was then used to detect primary binding. Nuclei were stained with Hoechst (blue). Expression levels of PPARγ, collagen type II, and osteocalcin (red) were lower in cells treated with 0.1 ng/ml PGE 2 ( A–C ) than those treated with the higher concentrations (10 and 100 ng/ml) of PGE 2 ( E–G, I–K ). H E staining was also performed on tissue sections ( D, H, L ). More cells (black dots; see insets in D , H , and L ) were observed in tissues implanted with hTSCs that had been treated with high concentrations of PGE 2 in culture ( H, L ). Specifically, at 100 ng/ml ( L ), cells were concentrated in a specific region (triangle). Additionally, semi-quantification of the stained cells was performed by counting immuno-positive cells and calculating percentage staining ( M ) (*p

    Journal: PLoS ONE

    Article Title: Prostaglandin E2 (PGE2) Exerts Biphasic Effects on Human Tendon Stem Cells

    doi: 10.1371/journal.pone.0087706

    Figure Lengend Snippet: In vivo expression of non-tenocyte markers PPARγ, collagen type II, and osteocalcin in rats implanted with hTSCs treated with various concentrations of PGE 2 and their respective hematoxylin and eosin (H E) stained tissue sections. hTSCs cultured with three concentrations (0.1, 10, and 100 ng/ml) of PGE 2 were implanted subcutaneously into rats; later, immunohistochemical and histological analyses were performed on tissue sections. For the immunohistochemical staining, fixed tissue sections were incubated with mouse anti-human PPARγ antibody, mouse anti-collagen type II (Collagen II) antibody, or mouse anti-human osteocalcin antibody. Cy3-conjugated goat anti-mouse IgG was then used to detect primary binding. Nuclei were stained with Hoechst (blue). Expression levels of PPARγ, collagen type II, and osteocalcin (red) were lower in cells treated with 0.1 ng/ml PGE 2 ( A–C ) than those treated with the higher concentrations (10 and 100 ng/ml) of PGE 2 ( E–G, I–K ). H E staining was also performed on tissue sections ( D, H, L ). More cells (black dots; see insets in D , H , and L ) were observed in tissues implanted with hTSCs that had been treated with high concentrations of PGE 2 in culture ( H, L ). Specifically, at 100 ng/ml ( L ), cells were concentrated in a specific region (triangle). Additionally, semi-quantification of the stained cells was performed by counting immuno-positive cells and calculating percentage staining ( M ) (*p

    Article Snippet: The cells were then washed three times with PBS, followed by incubation with Cy3-conjugated goat anti-mouse IgG (1∶500; Invitrogen, Cat. # A10521) secondary antibody at room temperature for 2 hrs.

    Techniques: In Vivo, Expressing, Staining, Cell Culture, Immunohistochemistry, Incubation, Binding Assay

    Induction of anti-HA humoral and cellular responses in mice vaccinated with VLP-HA/VSP-G. a – e Mice were orally vaccinated with VLPs and the humoral response against HA in different biological samples was evaluated. Levels of total serum anti-HA IgG ( a ), IgG1 and IgG2a ( b ), n = 8 from two independent experiments. Levels of IgA anti-HA in fecal extracts and BAL, n = 6 from two independent experiments ( c ). Levels of serum IgG anti-HA measured at different times post immunization, n = 6 from two independent experiments ( d ). Levels of serum IgG anti-HA in animals immunized with a mixture of VLP-HA plus VLP-VSP-G, n = 6 from two independent experiments ( e ). f Levels of serum IgG and fecal IgA in mice vaccinated with VLPs via oral or subcutaneous (s.c.) route, n = 8 from two independent experiments. g Mice were orally immunized with four weekly doses of 50 μg of recombinant ΔVSP1267 or vehicle. One week after the last immunization the presence of anti-VSP1267 antibodies in blood and fecal samples was checked, then these animals were orally immunized with VLP-HA/VSP-G, according to the protocol previously used. One week after the last VLP dose, the levels of serum IgG and fecal IgA anti-HA Abs were measured, n = 6 from two independent experiments. h , i Mice were orally vaccinated with VLPs and the cellular response against HA was evaluated. The frequency of HA-specific IFN-γ-secreting T cells (spot-forming colonies, SFC) was determined after antigen-specific re-stimulation of splenocytes, n = 7 from two independent experiments ( h ). Cytokines were measured in splenocyte supernatants and only those cytokines with detectable levels are shown, n = 6 from two independent experiments ( i ). Data were analyzed by one-way ANOVA and Tukey’s multiple comparison test ( a – f , h , i ) or by two-way ANOVA and Bonferroni post-tests ( g ). Values represent mean ± s.e.m. * p

    Journal: Nature Communications

    Article Title: Efficient oral vaccination by bioengineering virus-like particles with protozoan surface proteins

    doi: 10.1038/s41467-018-08265-9

    Figure Lengend Snippet: Induction of anti-HA humoral and cellular responses in mice vaccinated with VLP-HA/VSP-G. a – e Mice were orally vaccinated with VLPs and the humoral response against HA in different biological samples was evaluated. Levels of total serum anti-HA IgG ( a ), IgG1 and IgG2a ( b ), n = 8 from two independent experiments. Levels of IgA anti-HA in fecal extracts and BAL, n = 6 from two independent experiments ( c ). Levels of serum IgG anti-HA measured at different times post immunization, n = 6 from two independent experiments ( d ). Levels of serum IgG anti-HA in animals immunized with a mixture of VLP-HA plus VLP-VSP-G, n = 6 from two independent experiments ( e ). f Levels of serum IgG and fecal IgA in mice vaccinated with VLPs via oral or subcutaneous (s.c.) route, n = 8 from two independent experiments. g Mice were orally immunized with four weekly doses of 50 μg of recombinant ΔVSP1267 or vehicle. One week after the last immunization the presence of anti-VSP1267 antibodies in blood and fecal samples was checked, then these animals were orally immunized with VLP-HA/VSP-G, according to the protocol previously used. One week after the last VLP dose, the levels of serum IgG and fecal IgA anti-HA Abs were measured, n = 6 from two independent experiments. h , i Mice were orally vaccinated with VLPs and the cellular response against HA was evaluated. The frequency of HA-specific IFN-γ-secreting T cells (spot-forming colonies, SFC) was determined after antigen-specific re-stimulation of splenocytes, n = 7 from two independent experiments ( h ). Cytokines were measured in splenocyte supernatants and only those cytokines with detectable levels are shown, n = 6 from two independent experiments ( i ). Data were analyzed by one-way ANOVA and Tukey’s multiple comparison test ( a – f , h , i ) or by two-way ANOVA and Bonferroni post-tests ( g ). Values represent mean ± s.e.m. * p

    Article Snippet: The following horseradish peroxidase-conjugated antibodies were used: goat anti-mouse IgG1 (SouthernBiotech, Cat. # 1070-05, dilution 1/2000); goat anti-mouse IgG2 (SouthernBiotech, Cat. # 1080-05, dilution 1/2000); goat anti-mouse IgG (Molecular Probes, Cat. # G-21040, dilution 1/2000).

    Techniques: Mouse Assay, Recombinant

    Immunoglobulin from healthy calves. (A) BAL sample; (B) blood serum. Means ± SEM from 10 calves during the 3 to 6 mo of life. *Statistical difference for IgG1 BAL (T6 to T1, T2, and T3, P = 0.005).

    Journal: Journal of Animal Science

    Article Title: Alveolar macrophage functions during the transition phase to active immunity in calves

    doi: 10.1093/jas/sky261

    Figure Lengend Snippet: Immunoglobulin from healthy calves. (A) BAL sample; (B) blood serum. Means ± SEM from 10 calves during the 3 to 6 mo of life. *Statistical difference for IgG1 BAL (T6 to T1, T2, and T3, P = 0.005).

    Article Snippet: Subsequently, we marked the surface molecules CD14 (anti-bovine CD14 antibody, Cat. No. A10530, Life Technologies, San Diego, CA, USA) and IgG1-APC (Cat. No. A10530, Life Technologies, San Diego, CA, USA) for 30 min each.

    Techniques:

    Indirect fluorescence for binding of serum antibody to normal brain tissue. Serum from EAE affected WT mice show diffuse, strong white matter associated signal consistent with the presence of MOG specific IgG1. By contrast, corresponding signal is absent using serum from MOG induced KO mice, or healthy controls. There is no difference of signal intensity or distribution for IgM binding to brain, regardless of MOG induction or genotype. Representative images are shown.

    Journal: Autoimmunity

    Article Title: Critical role of activation induced cytidine deaminase in Experimental Autoimmune Encephalomyelitis

    doi: 10.3109/08916934.2012.750301

    Figure Lengend Snippet: Indirect fluorescence for binding of serum antibody to normal brain tissue. Serum from EAE affected WT mice show diffuse, strong white matter associated signal consistent with the presence of MOG specific IgG1. By contrast, corresponding signal is absent using serum from MOG induced KO mice, or healthy controls. There is no difference of signal intensity or distribution for IgM binding to brain, regardless of MOG induction or genotype. Representative images are shown.

    Article Snippet: After washing with PBS, binding of IgG1 or IgM on brain tissue was detected using IgG1 and IgM specific secondary antibodies (Invitrogen, A21121 and A21042; 5 ug/ml) conjugated to Alexa Fluor 488.

    Techniques: Fluorescence, Binding Assay, Mouse Assay

    Generation and functional characterization of Aicda deficient mice. A) Gene targeting strategy used to delete the catalytic domain (exon-3) of Aicda locus. The structure of the AID domains is shown in top. The location of the homology arms in the targeting vector is indicated. The schematic of targeting construct used to delete the catalytic domain of AID (exon-3) is shown in the middle. The solid triangles represent Cre recombination sites and the Neomycin cassette was subsequently removed by expression of Cre-recombinase. Representative WT or KO PCR products using 3′ or 5′ arm analysis at the Aicda locus is shown in the right panel. The size of Aicda KO PCR product is indicated and corresponds to the modified locus. Notice that one primer is located in the Neomycin cassette and therefore only detects the targeted Aicda locus. B) FACS analysis of LPS/IL4 stimulated WT or AID KO B cells in vitro. Percentage of IgM or IgG1 are indicated in each quadrant. B220-FITC is used as a B-cell marker.

    Journal: Autoimmunity

    Article Title: Critical role of activation induced cytidine deaminase in Experimental Autoimmune Encephalomyelitis

    doi: 10.3109/08916934.2012.750301

    Figure Lengend Snippet: Generation and functional characterization of Aicda deficient mice. A) Gene targeting strategy used to delete the catalytic domain (exon-3) of Aicda locus. The structure of the AID domains is shown in top. The location of the homology arms in the targeting vector is indicated. The schematic of targeting construct used to delete the catalytic domain of AID (exon-3) is shown in the middle. The solid triangles represent Cre recombination sites and the Neomycin cassette was subsequently removed by expression of Cre-recombinase. Representative WT or KO PCR products using 3′ or 5′ arm analysis at the Aicda locus is shown in the right panel. The size of Aicda KO PCR product is indicated and corresponds to the modified locus. Notice that one primer is located in the Neomycin cassette and therefore only detects the targeted Aicda locus. B) FACS analysis of LPS/IL4 stimulated WT or AID KO B cells in vitro. Percentage of IgM or IgG1 are indicated in each quadrant. B220-FITC is used as a B-cell marker.

    Article Snippet: After washing with PBS, binding of IgG1 or IgM on brain tissue was detected using IgG1 and IgM specific secondary antibodies (Invitrogen, A21121 and A21042; 5 ug/ml) conjugated to Alexa Fluor 488.

    Techniques: Functional Assay, Mouse Assay, Plasmid Preparation, Construct, Expressing, Polymerase Chain Reaction, Modification, FACS, In Vitro, Marker

    Characterization of anti-MOG antibodies using ELISA at day zero and 28. A) IgG anti-rhMOG, B) IgM anti-rhMOG, C) IgG anti-MOG peptide 35–55, D) IgM anti-MOG peptide 35–55. Mixed day 28 sera of WT or KO immunized mice were used as standard (arbitrarily defined as 100 U) for IgG or IgM antibodies, respectively. E) IgM binding to rhMOG coated at different concentrations. Sera from 8 mice were pooled for each of the following sample sets: Day 0 WT, Day 0 KO, Day 28 WT, and Day 28 KO. Pooled mice sera IgM Abs normalized at 0.1 mg/ml in the assay. Anti-mouse IgM was used as detection to exclusively measure the binding of IgM antibodies in serum.

    Journal: Autoimmunity

    Article Title: Critical role of activation induced cytidine deaminase in Experimental Autoimmune Encephalomyelitis

    doi: 10.3109/08916934.2012.750301

    Figure Lengend Snippet: Characterization of anti-MOG antibodies using ELISA at day zero and 28. A) IgG anti-rhMOG, B) IgM anti-rhMOG, C) IgG anti-MOG peptide 35–55, D) IgM anti-MOG peptide 35–55. Mixed day 28 sera of WT or KO immunized mice were used as standard (arbitrarily defined as 100 U) for IgG or IgM antibodies, respectively. E) IgM binding to rhMOG coated at different concentrations. Sera from 8 mice were pooled for each of the following sample sets: Day 0 WT, Day 0 KO, Day 28 WT, and Day 28 KO. Pooled mice sera IgM Abs normalized at 0.1 mg/ml in the assay. Anti-mouse IgM was used as detection to exclusively measure the binding of IgM antibodies in serum.

    Article Snippet: After washing with PBS, binding of IgG1 or IgM on brain tissue was detected using IgG1 and IgM specific secondary antibodies (Invitrogen, A21121 and A21042; 5 ug/ml) conjugated to Alexa Fluor 488.

    Techniques: Enzyme-linked Immunosorbent Assay, Mouse Assay, Binding Assay