goat anti-mouse igg Search Results


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  • 99
    Vector Laboratories biotinylated goat anti mouse igg
    Biotinylated Goat Anti Mouse Igg, supplied by Vector Laboratories, used in various techniques. Bioz Stars score: 99/100, based on 2266 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    LI-COR irdye 800cw goat anti mouse igg h l
    Irdye 800cw Goat Anti Mouse Igg H L, supplied by LI-COR, used in various techniques. Bioz Stars score: 99/100, based on 2660 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    Thermo Fisher goat anti mouse igg h l cross adsorbed secondary antibody
    Goat Anti Mouse Igg H L Cross Adsorbed Secondary Antibody, supplied by Thermo Fisher, used in various techniques. Bioz Stars score: 99/100, based on 14216 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    Thermo Fisher goat anti mouse igg h l highly cross adsorbed secondary antibody
    Goat Anti Mouse Igg H L Highly Cross Adsorbed Secondary Antibody, supplied by Thermo Fisher, used in various techniques. Bioz Stars score: 99/100, based on 9004 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    Thermo Fisher goat anti mouse igg
    Goat Anti Mouse Igg, supplied by Thermo Fisher, used in various techniques. Bioz Stars score: 99/100, based on 9708 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    Santa Cruz Biotechnology goat anti mouse igg hrp
    Immunoblots of TFF1, TFF2 and TFF3 in a normal cell line and three lung cancer cell lines. (A) Total proteins were harvested, separated on SDS-PAGE gels, and subjected to immunoblot analyses. The primary antibodies against TFF1, TFF2, TFF3 and actin were purchased from Santa Cruz Biotechnology (USA). Secondary antibodies were donkey anti-goat <t>IgG-HRP</t> (cat# sc-2020, 1:5,000, Santa Cruz Biotechnology) and goat anti-mouse IgG-HRP (cat# sc-2005, 1:10,000, Santa Cruz Biotechnology). Bound antibodies were detected using the ECL system (Pierce Biotechnology). The size of the TFF proteins were approximately 7–10 kDa. Experiments were repeated more than 3 times. Histograms show mean normalized OD of TFF protein bands relative to the OD of actin band. Error bars show standard error of the mean (SEM) (P
    Goat Anti Mouse Igg Hrp, supplied by Santa Cruz Biotechnology, used in various techniques. Bioz Stars score: 94/100, based on 4245 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    Jackson Immuno peroxidase affinipure goat anti mouse igg
    Immunoblots of TFF1, TFF2 and TFF3 in a normal cell line and three lung cancer cell lines. (A) Total proteins were harvested, separated on SDS-PAGE gels, and subjected to immunoblot analyses. The primary antibodies against TFF1, TFF2, TFF3 and actin were purchased from Santa Cruz Biotechnology (USA). Secondary antibodies were donkey anti-goat <t>IgG-HRP</t> (cat# sc-2020, 1:5,000, Santa Cruz Biotechnology) and goat anti-mouse IgG-HRP (cat# sc-2005, 1:10,000, Santa Cruz Biotechnology). Bound antibodies were detected using the ECL system (Pierce Biotechnology). The size of the TFF proteins were approximately 7–10 kDa. Experiments were repeated more than 3 times. Histograms show mean normalized OD of TFF protein bands relative to the OD of actin band. Error bars show standard error of the mean (SEM) (P
    Peroxidase Affinipure Goat Anti Mouse Igg, supplied by Jackson Immuno, used in various techniques. Bioz Stars score: 99/100, based on 3302 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    Millipore anti mouse igg whole molecule peroxidase antibody
    Immunoblots of TFF1, TFF2 and TFF3 in a normal cell line and three lung cancer cell lines. (A) Total proteins were harvested, separated on SDS-PAGE gels, and subjected to immunoblot analyses. The primary antibodies against TFF1, TFF2, TFF3 and actin were purchased from Santa Cruz Biotechnology (USA). Secondary antibodies were donkey anti-goat <t>IgG-HRP</t> (cat# sc-2020, 1:5,000, Santa Cruz Biotechnology) and goat anti-mouse IgG-HRP (cat# sc-2005, 1:10,000, Santa Cruz Biotechnology). Bound antibodies were detected using the ECL system (Pierce Biotechnology). The size of the TFF proteins were approximately 7–10 kDa. Experiments were repeated more than 3 times. Histograms show mean normalized OD of TFF protein bands relative to the OD of actin band. Error bars show standard error of the mean (SEM) (P
    Anti Mouse Igg Whole Molecule Peroxidase Antibody, supplied by Millipore, used in various techniques. Bioz Stars score: 99/100, based on 5324 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    Santa Cruz Biotechnology goat anti mouse igg
    <t>HCt/E-specific</t> antibody production in mice immunized with BoNT/HCt/E DNA vaccine by intradermal electroporation. Mice were immunized 3 times (week 0, 2, 4) with 50 μg of DNA vaccine. Anti-HCt/E <t>IgG</t> levels are expressed as endpoint titers. Each symbol represents data from an individual mouse. The bar represents the mean titer for each group.
    Goat Anti Mouse Igg, supplied by Santa Cruz Biotechnology, used in various techniques. Bioz Stars score: 93/100, based on 2624 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    SouthernBiotech goat anti mouse igg1
    Induction of anti-HA humoral and cellular responses in mice vaccinated with VLP-HA/VSP-G. a – e Mice were orally vaccinated with VLPs and the humoral response against HA in different biological samples was evaluated. Levels of total serum anti-HA IgG ( a ), <t>IgG1</t> and IgG2a ( b ), n = 8 from two independent experiments. Levels of IgA anti-HA in fecal extracts and BAL, n = 6 from two independent experiments ( c ). Levels of serum IgG anti-HA measured at different times post immunization, n = 6 from two independent experiments ( d ). Levels of serum IgG anti-HA in animals immunized with a mixture of VLP-HA plus VLP-VSP-G, n = 6 from two independent experiments ( e ). f Levels of serum IgG and fecal IgA in mice vaccinated with VLPs via oral or subcutaneous (s.c.) route, n = 8 from two independent experiments. g Mice were orally immunized with four weekly doses of 50 μg of recombinant ΔVSP1267 or vehicle. One week after the last immunization the presence of anti-VSP1267 antibodies in blood and fecal samples was checked, then these animals were orally immunized with VLP-HA/VSP-G, according to the protocol previously used. One week after the last VLP dose, the levels of serum IgG and fecal IgA anti-HA Abs were measured, n = 6 from two independent experiments. h , i Mice were orally vaccinated with VLPs and the cellular response against HA was evaluated. The frequency of HA-specific IFN-γ-secreting T cells (spot-forming colonies, SFC) was determined after antigen-specific re-stimulation of splenocytes, n = 7 from two independent experiments ( h ). Cytokines were measured in splenocyte supernatants and only those cytokines with detectable levels are shown, n = 6 from two independent experiments ( i ). Data were analyzed by one-way ANOVA and Tukey’s multiple comparison test ( a – f , h , i ) or by two-way ANOVA and Bonferroni post-tests ( g ). Values represent mean ± s.e.m. * p
    Goat Anti Mouse Igg1, supplied by SouthernBiotech, used in various techniques. Bioz Stars score: 99/100, based on 707 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    Thermo Fisher goat anti mouse igg1 cross adsorbed secondary antibody
    Antibody response and GC defects caused by Prmt5 deficiency. a – i Cγ1-cre (Ctrl) and Prmt5 F/F Cγ1-cre (F/F) mice were used throughout. a Total anti-NP <t>IgG1</t> in the serum of mice, measured by ELISA 14 days after NP-CGG immunization. Mean ± s.d. OD values for serial dilutions are plotted for n mice from three experiments. b Representative pictures of ELISPOT for NP-specific IgG1 antibody secreting cells (ASC) at day 14 post-immunization. The number of ASC of individual mice (symbols) and means (bars) are plotted. c Anti-NP IgG1 in the serum of mice at various times post-immunization. Mean ± s.d. values for n mice at each time point from two experiments are plotted. d Total levels of antibody isotypes in the serum of n non-immunized mice. e Mean + s.d. number of lymphocytes per spleen at day 14 post-immunization, enumerated by flow cytometry for n mice from two experiments. f Representative flow cytometry plots (gated on B220 + ) of splenic GC B cell proportions at 14 days post-immunization with NP-CGG. The number of GC B cells per spleen for individual mice (symbol) and medians (bars) from three experiments are plotted. g As in f , for MLN of mice infected with H. polygyrus for 14 days. Data from two experiments are plotted. h Mean + s.d. number of lymphocytes per MLN in the mice from g . i Representative IF in MLN from mice infected with H. polygyrus from g , stained for the indicated antigens. Scale bar, 100 µm. GC numbers per MLN scored in individual mice (symbols) are plotted to the right. p -Values throughout are by an unpaired, two-tailed Student's t test
    Goat Anti Mouse Igg1 Cross Adsorbed Secondary Antibody, supplied by Thermo Fisher, used in various techniques. Bioz Stars score: 99/100, based on 1214 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    Bio-Rad goat anti mouse
    Antibody response and GC defects caused by Prmt5 deficiency. a – i Cγ1-cre (Ctrl) and Prmt5 F/F Cγ1-cre (F/F) mice were used throughout. a Total anti-NP <t>IgG1</t> in the serum of mice, measured by ELISA 14 days after NP-CGG immunization. Mean ± s.d. OD values for serial dilutions are plotted for n mice from three experiments. b Representative pictures of ELISPOT for NP-specific IgG1 antibody secreting cells (ASC) at day 14 post-immunization. The number of ASC of individual mice (symbols) and means (bars) are plotted. c Anti-NP IgG1 in the serum of mice at various times post-immunization. Mean ± s.d. values for n mice at each time point from two experiments are plotted. d Total levels of antibody isotypes in the serum of n non-immunized mice. e Mean + s.d. number of lymphocytes per spleen at day 14 post-immunization, enumerated by flow cytometry for n mice from two experiments. f Representative flow cytometry plots (gated on B220 + ) of splenic GC B cell proportions at 14 days post-immunization with NP-CGG. The number of GC B cells per spleen for individual mice (symbol) and medians (bars) from three experiments are plotted. g As in f , for MLN of mice infected with H. polygyrus for 14 days. Data from two experiments are plotted. h Mean + s.d. number of lymphocytes per MLN in the mice from g . i Representative IF in MLN from mice infected with H. polygyrus from g , stained for the indicated antigens. Scale bar, 100 µm. GC numbers per MLN scored in individual mice (symbols) are plotted to the right. p -Values throughout are by an unpaired, two-tailed Student's t test
    Goat Anti Mouse, supplied by Bio-Rad, used in various techniques. Bioz Stars score: 99/100, based on 1880 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    Thermo Fisher alexa fluor 594 goat anti mouse igg
    Antibody response and GC defects caused by Prmt5 deficiency. a – i Cγ1-cre (Ctrl) and Prmt5 F/F Cγ1-cre (F/F) mice were used throughout. a Total anti-NP <t>IgG1</t> in the serum of mice, measured by ELISA 14 days after NP-CGG immunization. Mean ± s.d. OD values for serial dilutions are plotted for n mice from three experiments. b Representative pictures of ELISPOT for NP-specific IgG1 antibody secreting cells (ASC) at day 14 post-immunization. The number of ASC of individual mice (symbols) and means (bars) are plotted. c Anti-NP IgG1 in the serum of mice at various times post-immunization. Mean ± s.d. values for n mice at each time point from two experiments are plotted. d Total levels of antibody isotypes in the serum of n non-immunized mice. e Mean + s.d. number of lymphocytes per spleen at day 14 post-immunization, enumerated by flow cytometry for n mice from two experiments. f Representative flow cytometry plots (gated on B220 + ) of splenic GC B cell proportions at 14 days post-immunization with NP-CGG. The number of GC B cells per spleen for individual mice (symbol) and medians (bars) from three experiments are plotted. g As in f , for MLN of mice infected with H. polygyrus for 14 days. Data from two experiments are plotted. h Mean + s.d. number of lymphocytes per MLN in the mice from g . i Representative IF in MLN from mice infected with H. polygyrus from g , stained for the indicated antigens. Scale bar, 100 µm. GC numbers per MLN scored in individual mice (symbols) are plotted to the right. p -Values throughout are by an unpaired, two-tailed Student's t test
    Alexa Fluor 594 Goat Anti Mouse Igg, supplied by Thermo Fisher, used in various techniques. Bioz Stars score: 99/100, based on 2504 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    Abcam goat anti mouse igg h l hrp
    Antibody response and GC defects caused by Prmt5 deficiency. a – i Cγ1-cre (Ctrl) and Prmt5 F/F Cγ1-cre (F/F) mice were used throughout. a Total anti-NP <t>IgG1</t> in the serum of mice, measured by ELISA 14 days after NP-CGG immunization. Mean ± s.d. OD values for serial dilutions are plotted for n mice from three experiments. b Representative pictures of ELISPOT for NP-specific IgG1 antibody secreting cells (ASC) at day 14 post-immunization. The number of ASC of individual mice (symbols) and means (bars) are plotted. c Anti-NP IgG1 in the serum of mice at various times post-immunization. Mean ± s.d. values for n mice at each time point from two experiments are plotted. d Total levels of antibody isotypes in the serum of n non-immunized mice. e Mean + s.d. number of lymphocytes per spleen at day 14 post-immunization, enumerated by flow cytometry for n mice from two experiments. f Representative flow cytometry plots (gated on B220 + ) of splenic GC B cell proportions at 14 days post-immunization with NP-CGG. The number of GC B cells per spleen for individual mice (symbol) and medians (bars) from three experiments are plotted. g As in f , for MLN of mice infected with H. polygyrus for 14 days. Data from two experiments are plotted. h Mean + s.d. number of lymphocytes per MLN in the mice from g . i Representative IF in MLN from mice infected with H. polygyrus from g , stained for the indicated antigens. Scale bar, 100 µm. GC numbers per MLN scored in individual mice (symbols) are plotted to the right. p -Values throughout are by an unpaired, two-tailed Student's t test
    Goat Anti Mouse Igg H L Hrp, supplied by Abcam, used in various techniques. Bioz Stars score: 99/100, based on 729 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    Thermo Fisher goat anti mouse igg h l secondary antibody
    Antibody response and GC defects caused by Prmt5 deficiency. a – i Cγ1-cre (Ctrl) and Prmt5 F/F Cγ1-cre (F/F) mice were used throughout. a Total anti-NP <t>IgG1</t> in the serum of mice, measured by ELISA 14 days after NP-CGG immunization. Mean ± s.d. OD values for serial dilutions are plotted for n mice from three experiments. b Representative pictures of ELISPOT for NP-specific IgG1 antibody secreting cells (ASC) at day 14 post-immunization. The number of ASC of individual mice (symbols) and means (bars) are plotted. c Anti-NP IgG1 in the serum of mice at various times post-immunization. Mean ± s.d. values for n mice at each time point from two experiments are plotted. d Total levels of antibody isotypes in the serum of n non-immunized mice. e Mean + s.d. number of lymphocytes per spleen at day 14 post-immunization, enumerated by flow cytometry for n mice from two experiments. f Representative flow cytometry plots (gated on B220 + ) of splenic GC B cell proportions at 14 days post-immunization with NP-CGG. The number of GC B cells per spleen for individual mice (symbol) and medians (bars) from three experiments are plotted. g As in f , for MLN of mice infected with H. polygyrus for 14 days. Data from two experiments are plotted. h Mean + s.d. number of lymphocytes per MLN in the mice from g . i Representative IF in MLN from mice infected with H. polygyrus from g , stained for the indicated antigens. Scale bar, 100 µm. GC numbers per MLN scored in individual mice (symbols) are plotted to the right. p -Values throughout are by an unpaired, two-tailed Student's t test
    Goat Anti Mouse Igg H L Secondary Antibody, supplied by Thermo Fisher, used in various techniques. Bioz Stars score: 99/100, based on 953 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    Bio-Rad goat anti mouse igg
    Antibody response and GC defects caused by Prmt5 deficiency. a – i Cγ1-cre (Ctrl) and Prmt5 F/F Cγ1-cre (F/F) mice were used throughout. a Total anti-NP <t>IgG1</t> in the serum of mice, measured by ELISA 14 days after NP-CGG immunization. Mean ± s.d. OD values for serial dilutions are plotted for n mice from three experiments. b Representative pictures of ELISPOT for NP-specific IgG1 antibody secreting cells (ASC) at day 14 post-immunization. The number of ASC of individual mice (symbols) and means (bars) are plotted. c Anti-NP IgG1 in the serum of mice at various times post-immunization. Mean ± s.d. values for n mice at each time point from two experiments are plotted. d Total levels of antibody isotypes in the serum of n non-immunized mice. e Mean + s.d. number of lymphocytes per spleen at day 14 post-immunization, enumerated by flow cytometry for n mice from two experiments. f Representative flow cytometry plots (gated on B220 + ) of splenic GC B cell proportions at 14 days post-immunization with NP-CGG. The number of GC B cells per spleen for individual mice (symbol) and medians (bars) from three experiments are plotted. g As in f , for MLN of mice infected with H. polygyrus for 14 days. Data from two experiments are plotted. h Mean + s.d. number of lymphocytes per MLN in the mice from g . i Representative IF in MLN from mice infected with H. polygyrus from g , stained for the indicated antigens. Scale bar, 100 µm. GC numbers per MLN scored in individual mice (symbols) are plotted to the right. p -Values throughout are by an unpaired, two-tailed Student's t test
    Goat Anti Mouse Igg, supplied by Bio-Rad, used in various techniques. Bioz Stars score: 98/100, based on 1229 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    LI-COR irdye 680rd goat anti mouse igg h l
    Antibody response and GC defects caused by Prmt5 deficiency. a – i Cγ1-cre (Ctrl) and Prmt5 F/F Cγ1-cre (F/F) mice were used throughout. a Total anti-NP <t>IgG1</t> in the serum of mice, measured by ELISA 14 days after NP-CGG immunization. Mean ± s.d. OD values for serial dilutions are plotted for n mice from three experiments. b Representative pictures of ELISPOT for NP-specific IgG1 antibody secreting cells (ASC) at day 14 post-immunization. The number of ASC of individual mice (symbols) and means (bars) are plotted. c Anti-NP IgG1 in the serum of mice at various times post-immunization. Mean ± s.d. values for n mice at each time point from two experiments are plotted. d Total levels of antibody isotypes in the serum of n non-immunized mice. e Mean + s.d. number of lymphocytes per spleen at day 14 post-immunization, enumerated by flow cytometry for n mice from two experiments. f Representative flow cytometry plots (gated on B220 + ) of splenic GC B cell proportions at 14 days post-immunization with NP-CGG. The number of GC B cells per spleen for individual mice (symbol) and medians (bars) from three experiments are plotted. g As in f , for MLN of mice infected with H. polygyrus for 14 days. Data from two experiments are plotted. h Mean + s.d. number of lymphocytes per MLN in the mice from g . i Representative IF in MLN from mice infected with H. polygyrus from g , stained for the indicated antigens. Scale bar, 100 µm. GC numbers per MLN scored in individual mice (symbols) are plotted to the right. p -Values throughout are by an unpaired, two-tailed Student's t test
    Irdye 680rd Goat Anti Mouse Igg H L, supplied by LI-COR, used in various techniques. Bioz Stars score: 99/100, based on 1203 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    95
    Millipore igg
    Antibody response and GC defects caused by Prmt5 deficiency. a – i Cγ1-cre (Ctrl) and Prmt5 F/F Cγ1-cre (F/F) mice were used throughout. a Total anti-NP <t>IgG1</t> in the serum of mice, measured by ELISA 14 days after NP-CGG immunization. Mean ± s.d. OD values for serial dilutions are plotted for n mice from three experiments. b Representative pictures of ELISPOT for NP-specific IgG1 antibody secreting cells (ASC) at day 14 post-immunization. The number of ASC of individual mice (symbols) and means (bars) are plotted. c Anti-NP IgG1 in the serum of mice at various times post-immunization. Mean ± s.d. values for n mice at each time point from two experiments are plotted. d Total levels of antibody isotypes in the serum of n non-immunized mice. e Mean + s.d. number of lymphocytes per spleen at day 14 post-immunization, enumerated by flow cytometry for n mice from two experiments. f Representative flow cytometry plots (gated on B220 + ) of splenic GC B cell proportions at 14 days post-immunization with NP-CGG. The number of GC B cells per spleen for individual mice (symbol) and medians (bars) from three experiments are plotted. g As in f , for MLN of mice infected with H. polygyrus for 14 days. Data from two experiments are plotted. h Mean + s.d. number of lymphocytes per MLN in the mice from g . i Representative IF in MLN from mice infected with H. polygyrus from g , stained for the indicated antigens. Scale bar, 100 µm. GC numbers per MLN scored in individual mice (symbols) are plotted to the right. p -Values throughout are by an unpaired, two-tailed Student's t test
    Igg, supplied by Millipore, used in various techniques. Bioz Stars score: 95/100, based on 10101 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    Thermo Fisher f ab 2 goat anti mouse igg h l cross adsorbed secondary antibody
    Antibody response and GC defects caused by Prmt5 deficiency. a – i Cγ1-cre (Ctrl) and Prmt5 F/F Cγ1-cre (F/F) mice were used throughout. a Total anti-NP <t>IgG1</t> in the serum of mice, measured by ELISA 14 days after NP-CGG immunization. Mean ± s.d. OD values for serial dilutions are plotted for n mice from three experiments. b Representative pictures of ELISPOT for NP-specific IgG1 antibody secreting cells (ASC) at day 14 post-immunization. The number of ASC of individual mice (symbols) and means (bars) are plotted. c Anti-NP IgG1 in the serum of mice at various times post-immunization. Mean ± s.d. values for n mice at each time point from two experiments are plotted. d Total levels of antibody isotypes in the serum of n non-immunized mice. e Mean + s.d. number of lymphocytes per spleen at day 14 post-immunization, enumerated by flow cytometry for n mice from two experiments. f Representative flow cytometry plots (gated on B220 + ) of splenic GC B cell proportions at 14 days post-immunization with NP-CGG. The number of GC B cells per spleen for individual mice (symbol) and medians (bars) from three experiments are plotted. g As in f , for MLN of mice infected with H. polygyrus for 14 days. Data from two experiments are plotted. h Mean + s.d. number of lymphocytes per MLN in the mice from g . i Representative IF in MLN from mice infected with H. polygyrus from g , stained for the indicated antigens. Scale bar, 100 µm. GC numbers per MLN scored in individual mice (symbols) are plotted to the right. p -Values throughout are by an unpaired, two-tailed Student's t test
    F Ab 2 Goat Anti Mouse Igg H L Cross Adsorbed Secondary Antibody, supplied by Thermo Fisher, used in various techniques. Bioz Stars score: 99/100, based on 1371 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    99
    Abcam goat anti mouse igg
    Antibody response and GC defects caused by Prmt5 deficiency. a – i Cγ1-cre (Ctrl) and Prmt5 F/F Cγ1-cre (F/F) mice were used throughout. a Total anti-NP <t>IgG1</t> in the serum of mice, measured by ELISA 14 days after NP-CGG immunization. Mean ± s.d. OD values for serial dilutions are plotted for n mice from three experiments. b Representative pictures of ELISPOT for NP-specific IgG1 antibody secreting cells (ASC) at day 14 post-immunization. The number of ASC of individual mice (symbols) and means (bars) are plotted. c Anti-NP IgG1 in the serum of mice at various times post-immunization. Mean ± s.d. values for n mice at each time point from two experiments are plotted. d Total levels of antibody isotypes in the serum of n non-immunized mice. e Mean + s.d. number of lymphocytes per spleen at day 14 post-immunization, enumerated by flow cytometry for n mice from two experiments. f Representative flow cytometry plots (gated on B220 + ) of splenic GC B cell proportions at 14 days post-immunization with NP-CGG. The number of GC B cells per spleen for individual mice (symbol) and medians (bars) from three experiments are plotted. g As in f , for MLN of mice infected with H. polygyrus for 14 days. Data from two experiments are plotted. h Mean + s.d. number of lymphocytes per MLN in the mice from g . i Representative IF in MLN from mice infected with H. polygyrus from g , stained for the indicated antigens. Scale bar, 100 µm. GC numbers per MLN scored in individual mice (symbols) are plotted to the right. p -Values throughout are by an unpaired, two-tailed Student's t test
    Goat Anti Mouse Igg, supplied by Abcam, used in various techniques. Bioz Stars score: 99/100, based on 1173 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    91
    Santa Cruz Biotechnology hrp conjugated goat anti mouse igg1
    Specific binding ELISA of hE16 to plant-derived DIII. Serial dilutions of hE16 purified from mammalian or plant cells were incubated in sample wells coated with plant-produced WNV DIII and detected with an <t>HRP-conjugated</t> anti-human gamma antibody. A commercial generic human <t>IgG</t> was used as a negative control. Mean ± SD of samples from three independent experiments is presented.
    Hrp Conjugated Goat Anti Mouse Igg1, supplied by Santa Cruz Biotechnology, used in various techniques. Bioz Stars score: 91/100, based on 22 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    Image Search Results


    Immunoblots of TFF1, TFF2 and TFF3 in a normal cell line and three lung cancer cell lines. (A) Total proteins were harvested, separated on SDS-PAGE gels, and subjected to immunoblot analyses. The primary antibodies against TFF1, TFF2, TFF3 and actin were purchased from Santa Cruz Biotechnology (USA). Secondary antibodies were donkey anti-goat IgG-HRP (cat# sc-2020, 1:5,000, Santa Cruz Biotechnology) and goat anti-mouse IgG-HRP (cat# sc-2005, 1:10,000, Santa Cruz Biotechnology). Bound antibodies were detected using the ECL system (Pierce Biotechnology). The size of the TFF proteins were approximately 7–10 kDa. Experiments were repeated more than 3 times. Histograms show mean normalized OD of TFF protein bands relative to the OD of actin band. Error bars show standard error of the mean (SEM) (P

    Journal: Oncology Reports

    Article Title: Increased trefoil factor 3 levels in the serum of patients with three major histological subtypes of lung cancer

    doi: 10.3892/or.2012.1627

    Figure Lengend Snippet: Immunoblots of TFF1, TFF2 and TFF3 in a normal cell line and three lung cancer cell lines. (A) Total proteins were harvested, separated on SDS-PAGE gels, and subjected to immunoblot analyses. The primary antibodies against TFF1, TFF2, TFF3 and actin were purchased from Santa Cruz Biotechnology (USA). Secondary antibodies were donkey anti-goat IgG-HRP (cat# sc-2020, 1:5,000, Santa Cruz Biotechnology) and goat anti-mouse IgG-HRP (cat# sc-2005, 1:10,000, Santa Cruz Biotechnology). Bound antibodies were detected using the ECL system (Pierce Biotechnology). The size of the TFF proteins were approximately 7–10 kDa. Experiments were repeated more than 3 times. Histograms show mean normalized OD of TFF protein bands relative to the OD of actin band. Error bars show standard error of the mean (SEM) (P

    Article Snippet: Secondary antibodies used in this study were donkey anti-goat IgG-HRP (cat# sc-2020, 1:5,000, Santa Cruz Biotechnology) and goat anti-mouse IgG-HRP (Cat# sc-2005, 1:10,000, Santa Cruz Biotechnology).

    Techniques: Western Blot, SDS Page

    Immunoblots of TFF1, TFF2 and TFF3 in healthy individuals and lung cancer patients. (A) Total proteins were extracted from lung tissues, separated on SDS-PAGE gels, and subjected to immunoblot analyses. The primary antibodies against TFF1, TFF2, TFF3 and actin were purchased from Santa Cruz Biotechnology (USA). Secondary antibodies were donkey anti-goat IgG-HRP (cat# sc-2020, 1:5,000, Santa Cruz Biotechnology) and goat anti-mouse IgG-HRP (cat# sc-2005, 1:10,000, Santa Cruz Biotechnology). Bound antibodies were detected using the ECL system (Pierce Biotechnology). The size of the TFF proteins was approximately 7–10 kDa. Histograms show mean normalized optical density (OD) of TFF protein bands relative to the OD of the actin band from the same individual. Error bars show the standard error of the mean (SEM) (P

    Journal: Oncology Reports

    Article Title: Increased trefoil factor 3 levels in the serum of patients with three major histological subtypes of lung cancer

    doi: 10.3892/or.2012.1627

    Figure Lengend Snippet: Immunoblots of TFF1, TFF2 and TFF3 in healthy individuals and lung cancer patients. (A) Total proteins were extracted from lung tissues, separated on SDS-PAGE gels, and subjected to immunoblot analyses. The primary antibodies against TFF1, TFF2, TFF3 and actin were purchased from Santa Cruz Biotechnology (USA). Secondary antibodies were donkey anti-goat IgG-HRP (cat# sc-2020, 1:5,000, Santa Cruz Biotechnology) and goat anti-mouse IgG-HRP (cat# sc-2005, 1:10,000, Santa Cruz Biotechnology). Bound antibodies were detected using the ECL system (Pierce Biotechnology). The size of the TFF proteins was approximately 7–10 kDa. Histograms show mean normalized optical density (OD) of TFF protein bands relative to the OD of the actin band from the same individual. Error bars show the standard error of the mean (SEM) (P

    Article Snippet: Secondary antibodies used in this study were donkey anti-goat IgG-HRP (cat# sc-2020, 1:5,000, Santa Cruz Biotechnology) and goat anti-mouse IgG-HRP (Cat# sc-2005, 1:10,000, Santa Cruz Biotechnology).

    Techniques: Western Blot, SDS Page

    HCt/E-specific antibody production in mice immunized with BoNT/HCt/E DNA vaccine by intradermal electroporation. Mice were immunized 3 times (week 0, 2, 4) with 50 μg of DNA vaccine. Anti-HCt/E IgG levels are expressed as endpoint titers. Each symbol represents data from an individual mouse. The bar represents the mean titer for each group.

    Journal: Human Vaccines & Immunotherapeutics

    Article Title: Intradermal immunization with botulinum neurotoxin serotype E DNA vaccine induces humoral and cellular immunity and protects against lethal toxin challenge

    doi: 10.1080/21645515.2018.1526554

    Figure Lengend Snippet: HCt/E-specific antibody production in mice immunized with BoNT/HCt/E DNA vaccine by intradermal electroporation. Mice were immunized 3 times (week 0, 2, 4) with 50 μg of DNA vaccine. Anti-HCt/E IgG levels are expressed as endpoint titers. Each symbol represents data from an individual mouse. The bar represents the mean titer for each group.

    Article Snippet: The HCt/E protein was detected using a mouse anti-HCt/E antibody and goat anti-mouse IgG conjugated to horseradish peroxidase (Santa Cruz Biotech, Santa Cruz, USA).

    Techniques: Mouse Assay, Electroporation

    Survival and antibody titers following immunization with HCt/E protein vaccine. Mice were immunized three times at 2-week intervals with 10 μg or 20 μg of purified HCt/E vaccine intramuscularly, followed by intraperitoneal challenge with 200 LD 50 of active BoNT/E. The number of survivors are shown. Anti-HCt/E IgG levels are expressed as endpoint titers. Sera were obtained one week after last immunization. Each symbol represents data from an individual mouse. The bar represents the mean titer for each group.

    Journal: Human Vaccines & Immunotherapeutics

    Article Title: Intradermal immunization with botulinum neurotoxin serotype E DNA vaccine induces humoral and cellular immunity and protects against lethal toxin challenge

    doi: 10.1080/21645515.2018.1526554

    Figure Lengend Snippet: Survival and antibody titers following immunization with HCt/E protein vaccine. Mice were immunized three times at 2-week intervals with 10 μg or 20 μg of purified HCt/E vaccine intramuscularly, followed by intraperitoneal challenge with 200 LD 50 of active BoNT/E. The number of survivors are shown. Anti-HCt/E IgG levels are expressed as endpoint titers. Sera were obtained one week after last immunization. Each symbol represents data from an individual mouse. The bar represents the mean titer for each group.

    Article Snippet: The HCt/E protein was detected using a mouse anti-HCt/E antibody and goat anti-mouse IgG conjugated to horseradish peroxidase (Santa Cruz Biotech, Santa Cruz, USA).

    Techniques: Mouse Assay, Purification

    Survival and antibody titers following immunization with BoNT/HCt/E DNA vaccine. Mice were immunized two or three times at 2-week intervals with various doses of DNA vaccine intradermally via electroporation, followed by intraperitoneal challenge with 50 LD 50 of active BoNT/E. (A) Survival curves show the percentage of mice alive after challenge. Data shown are from one of two experiments. (B) Anti-HCt/E IgG levels are expressed as endpoint titers. Sera were obtained one week before challenged. Each symbol represents data from an individual mouse. The bar represents the mean titer for each group. Control group mice were immunized with vector only. Significant results are marked asterisks: ** p

    Journal: Human Vaccines & Immunotherapeutics

    Article Title: Intradermal immunization with botulinum neurotoxin serotype E DNA vaccine induces humoral and cellular immunity and protects against lethal toxin challenge

    doi: 10.1080/21645515.2018.1526554

    Figure Lengend Snippet: Survival and antibody titers following immunization with BoNT/HCt/E DNA vaccine. Mice were immunized two or three times at 2-week intervals with various doses of DNA vaccine intradermally via electroporation, followed by intraperitoneal challenge with 50 LD 50 of active BoNT/E. (A) Survival curves show the percentage of mice alive after challenge. Data shown are from one of two experiments. (B) Anti-HCt/E IgG levels are expressed as endpoint titers. Sera were obtained one week before challenged. Each symbol represents data from an individual mouse. The bar represents the mean titer for each group. Control group mice were immunized with vector only. Significant results are marked asterisks: ** p

    Article Snippet: The HCt/E protein was detected using a mouse anti-HCt/E antibody and goat anti-mouse IgG conjugated to horseradish peroxidase (Santa Cruz Biotech, Santa Cruz, USA).

    Techniques: Mouse Assay, Electroporation, Plasmid Preparation

    Induction of anti-HA humoral and cellular responses in mice vaccinated with VLP-HA/VSP-G. a – e Mice were orally vaccinated with VLPs and the humoral response against HA in different biological samples was evaluated. Levels of total serum anti-HA IgG ( a ), IgG1 and IgG2a ( b ), n = 8 from two independent experiments. Levels of IgA anti-HA in fecal extracts and BAL, n = 6 from two independent experiments ( c ). Levels of serum IgG anti-HA measured at different times post immunization, n = 6 from two independent experiments ( d ). Levels of serum IgG anti-HA in animals immunized with a mixture of VLP-HA plus VLP-VSP-G, n = 6 from two independent experiments ( e ). f Levels of serum IgG and fecal IgA in mice vaccinated with VLPs via oral or subcutaneous (s.c.) route, n = 8 from two independent experiments. g Mice were orally immunized with four weekly doses of 50 μg of recombinant ΔVSP1267 or vehicle. One week after the last immunization the presence of anti-VSP1267 antibodies in blood and fecal samples was checked, then these animals were orally immunized with VLP-HA/VSP-G, according to the protocol previously used. One week after the last VLP dose, the levels of serum IgG and fecal IgA anti-HA Abs were measured, n = 6 from two independent experiments. h , i Mice were orally vaccinated with VLPs and the cellular response against HA was evaluated. The frequency of HA-specific IFN-γ-secreting T cells (spot-forming colonies, SFC) was determined after antigen-specific re-stimulation of splenocytes, n = 7 from two independent experiments ( h ). Cytokines were measured in splenocyte supernatants and only those cytokines with detectable levels are shown, n = 6 from two independent experiments ( i ). Data were analyzed by one-way ANOVA and Tukey’s multiple comparison test ( a – f , h , i ) or by two-way ANOVA and Bonferroni post-tests ( g ). Values represent mean ± s.e.m. * p

    Journal: Nature Communications

    Article Title: Efficient oral vaccination by bioengineering virus-like particles with protozoan surface proteins

    doi: 10.1038/s41467-018-08265-9

    Figure Lengend Snippet: Induction of anti-HA humoral and cellular responses in mice vaccinated with VLP-HA/VSP-G. a – e Mice were orally vaccinated with VLPs and the humoral response against HA in different biological samples was evaluated. Levels of total serum anti-HA IgG ( a ), IgG1 and IgG2a ( b ), n = 8 from two independent experiments. Levels of IgA anti-HA in fecal extracts and BAL, n = 6 from two independent experiments ( c ). Levels of serum IgG anti-HA measured at different times post immunization, n = 6 from two independent experiments ( d ). Levels of serum IgG anti-HA in animals immunized with a mixture of VLP-HA plus VLP-VSP-G, n = 6 from two independent experiments ( e ). f Levels of serum IgG and fecal IgA in mice vaccinated with VLPs via oral or subcutaneous (s.c.) route, n = 8 from two independent experiments. g Mice were orally immunized with four weekly doses of 50 μg of recombinant ΔVSP1267 or vehicle. One week after the last immunization the presence of anti-VSP1267 antibodies in blood and fecal samples was checked, then these animals were orally immunized with VLP-HA/VSP-G, according to the protocol previously used. One week after the last VLP dose, the levels of serum IgG and fecal IgA anti-HA Abs were measured, n = 6 from two independent experiments. h , i Mice were orally vaccinated with VLPs and the cellular response against HA was evaluated. The frequency of HA-specific IFN-γ-secreting T cells (spot-forming colonies, SFC) was determined after antigen-specific re-stimulation of splenocytes, n = 7 from two independent experiments ( h ). Cytokines were measured in splenocyte supernatants and only those cytokines with detectable levels are shown, n = 6 from two independent experiments ( i ). Data were analyzed by one-way ANOVA and Tukey’s multiple comparison test ( a – f , h , i ) or by two-way ANOVA and Bonferroni post-tests ( g ). Values represent mean ± s.e.m. * p

    Article Snippet: The following horseradish peroxidase-conjugated antibodies were used: goat anti-mouse IgG1 (SouthernBiotech, Cat. # 1070-05, dilution 1/2000); goat anti-mouse IgG2 (SouthernBiotech, Cat. # 1080-05, dilution 1/2000); goat anti-mouse IgG (Molecular Probes, Cat. # G-21040, dilution 1/2000).

    Techniques: Mouse Assay, Recombinant

    Antibody response and GC defects caused by Prmt5 deficiency. a – i Cγ1-cre (Ctrl) and Prmt5 F/F Cγ1-cre (F/F) mice were used throughout. a Total anti-NP IgG1 in the serum of mice, measured by ELISA 14 days after NP-CGG immunization. Mean ± s.d. OD values for serial dilutions are plotted for n mice from three experiments. b Representative pictures of ELISPOT for NP-specific IgG1 antibody secreting cells (ASC) at day 14 post-immunization. The number of ASC of individual mice (symbols) and means (bars) are plotted. c Anti-NP IgG1 in the serum of mice at various times post-immunization. Mean ± s.d. values for n mice at each time point from two experiments are plotted. d Total levels of antibody isotypes in the serum of n non-immunized mice. e Mean + s.d. number of lymphocytes per spleen at day 14 post-immunization, enumerated by flow cytometry for n mice from two experiments. f Representative flow cytometry plots (gated on B220 + ) of splenic GC B cell proportions at 14 days post-immunization with NP-CGG. The number of GC B cells per spleen for individual mice (symbol) and medians (bars) from three experiments are plotted. g As in f , for MLN of mice infected with H. polygyrus for 14 days. Data from two experiments are plotted. h Mean + s.d. number of lymphocytes per MLN in the mice from g . i Representative IF in MLN from mice infected with H. polygyrus from g , stained for the indicated antigens. Scale bar, 100 µm. GC numbers per MLN scored in individual mice (symbols) are plotted to the right. p -Values throughout are by an unpaired, two-tailed Student's t test

    Journal: Nature Communications

    Article Title: PRMT5 is essential for B cell development and germinal center dynamics

    doi: 10.1038/s41467-018-07884-6

    Figure Lengend Snippet: Antibody response and GC defects caused by Prmt5 deficiency. a – i Cγ1-cre (Ctrl) and Prmt5 F/F Cγ1-cre (F/F) mice were used throughout. a Total anti-NP IgG1 in the serum of mice, measured by ELISA 14 days after NP-CGG immunization. Mean ± s.d. OD values for serial dilutions are plotted for n mice from three experiments. b Representative pictures of ELISPOT for NP-specific IgG1 antibody secreting cells (ASC) at day 14 post-immunization. The number of ASC of individual mice (symbols) and means (bars) are plotted. c Anti-NP IgG1 in the serum of mice at various times post-immunization. Mean ± s.d. values for n mice at each time point from two experiments are plotted. d Total levels of antibody isotypes in the serum of n non-immunized mice. e Mean + s.d. number of lymphocytes per spleen at day 14 post-immunization, enumerated by flow cytometry for n mice from two experiments. f Representative flow cytometry plots (gated on B220 + ) of splenic GC B cell proportions at 14 days post-immunization with NP-CGG. The number of GC B cells per spleen for individual mice (symbol) and medians (bars) from three experiments are plotted. g As in f , for MLN of mice infected with H. polygyrus for 14 days. Data from two experiments are plotted. h Mean + s.d. number of lymphocytes per MLN in the mice from g . i Representative IF in MLN from mice infected with H. polygyrus from g , stained for the indicated antigens. Scale bar, 100 µm. GC numbers per MLN scored in individual mice (symbols) are plotted to the right. p -Values throughout are by an unpaired, two-tailed Student's t test

    Article Snippet: Plates with cells were incubated in a humid chamber 12 h at 37 °C, 5% CO2 , then washed 6× with PBS 0.01% Tween-20, followed by incubation with goat anti-mouse IgG1-HRP (A10551, Life Technologies, 1/2000) diluted in culture media for 2 h at RT.

    Techniques: Mouse Assay, Enzyme-linked Immunosorbent Assay, Enzyme-linked Immunospot, Flow Cytometry, Cytometry, Infection, Staining, Two Tailed Test

    Specific binding ELISA of hE16 to plant-derived DIII. Serial dilutions of hE16 purified from mammalian or plant cells were incubated in sample wells coated with plant-produced WNV DIII and detected with an HRP-conjugated anti-human gamma antibody. A commercial generic human IgG was used as a negative control. Mean ± SD of samples from three independent experiments is presented.

    Journal: BioMed Research International

    Article Title: A Plant-Produced Antigen Elicits Potent Immune Responses against West Nile Virus in Mice

    doi: 10.1155/2014/952865

    Figure Lengend Snippet: Specific binding ELISA of hE16 to plant-derived DIII. Serial dilutions of hE16 purified from mammalian or plant cells were incubated in sample wells coated with plant-produced WNV DIII and detected with an HRP-conjugated anti-human gamma antibody. A commercial generic human IgG was used as a negative control. Mean ± SD of samples from three independent experiments is presented.

    Article Snippet: After washing with PBST, the plates were incubated with an HRP-conjugated goat anti-mouse IgG1 (Santa Cruz Biotech) or anti-mouse IgG2a (Southern Biotech).

    Techniques: Binding Assay, Enzyme-linked Immunosorbent Assay, Derivative Assay, Purification, Incubation, Produced, Negative Control