Journal: PLoS ONE

Article Title: Autoantibodies against Phospholipase A 2 Receptor in Korean Patients with Membranous Nephropathy

doi: 10.1371/journal.pone.0062151

Figure Lengend Snippet: Human glomerular extracts were electrophoresed, and serum samples of idiopathic membranous nephropathy patients were used as the primary antibody at a dilution of 1∶100. The band detected by immunoblotting with commercial anti-PLA 2 R antibody was used to indicate the position of PLA 2 R. Serum samples from a number of patients with idiopathic MN (except MN5, MN9, and MN10) and the commercial anti-PLA 2 R antibody demonstrated positive bands at approximately 185kD.

Article Snippet: A goat polyclonal Ab against PLA 2 R (Sigma-Aldrich, St. Louis, MO, USA) was used at a dilution of 1∶400 to confirm the location of the PLA 2 R band.

Techniques: Western Blot

Journal: PLoS ONE

Article Title: Autoantibodies against Phospholipase A 2 Receptor in Korean Patients with Membranous Nephropathy

doi: 10.1371/journal.pone.0062151

Figure Lengend Snippet: A: Proteinuria was more severe in patients with higher anti-PLA 2 R level. *Dunn’s multiple pairwise comparison test after Kruskal-Wallis test. B Severity of hypoalbuminemia was increased depending on the anti-PLA 2 R levels. **ANOVA test with polynomial contrast. C: The proportion of patients with nephrotic range proteinuria was increased according to the anti-PLA 2 R levels. *** Mantel-Haenszel χ 2 test.

Article Snippet: A goat polyclonal Ab against PLA 2 R (Sigma-Aldrich, St. Louis, MO, USA) was used at a dilution of 1∶400 to confirm the location of the PLA 2 R band.

Techniques:

Journal: PLoS ONE

Article Title: Autoantibodies against Phospholipase A 2 Receptor in Korean Patients with Membranous Nephropathy

doi: 10.1371/journal.pone.0062151

Figure Lengend Snippet: The proportion of the high-risk group was gradually increased as anti-PLA 2 R levels increased. * Mantel-Haenszel χ 2 test.

Article Snippet: A goat polyclonal Ab against PLA 2 R (Sigma-Aldrich, St. Louis, MO, USA) was used at a dilution of 1∶400 to confirm the location of the PLA 2 R band.

Techniques:

Journal: Frontiers in Immunology

Article Title: Phospholipase A2 Receptor Autoantibodies as a Novel Serological Biomarker for Autoimmune Thyroid Disease Associated Nephropathy

doi: 10.3389/fimmu.2020.00837

Figure Lengend Snippet: Standard curve of anti-PLA2R-IgG-TRFIA.

Article Snippet: Goat anti-PLA2R antibodies were purchased from Abcam (Cambridge, UK).

Techniques:

Journal: Frontiers in Immunology

Article Title: Phospholipase A2 Receptor Autoantibodies as a Novel Serological Biomarker for Autoimmune Thyroid Disease Associated Nephropathy

doi: 10.3389/fimmu.2020.00837

Figure Lengend Snippet: Anti-PLA2R-IgG detection in the serum of patients. Sera from 46 patients with IMN, 40 patients with HT, 47 patients with Graves' disease, 10 patients with nodular goiter, and 64 healthy controls were examined.

Article Snippet: Goat anti-PLA2R antibodies were purchased from Abcam (Cambridge, UK).

Techniques:

Journal: Frontiers in Immunology

Article Title: Phospholipase A2 Receptor Autoantibodies as a Novel Serological Biomarker for Autoimmune Thyroid Disease Associated Nephropathy

doi: 10.3389/fimmu.2020.00837

Figure Lengend Snippet: Characteristics of healthy volunteers and different patients with thyroid disease or IMN.

Article Snippet: Goat anti-PLA2R antibodies were purchased from Abcam (Cambridge, UK).

Techniques: