goat anti mouse immunoglobulin g igg Search Results


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  • 86
    Thermo Fisher goat anti mouse immunoglobulin g
    NM exposure reduces native FGF-1 levels in corneas cultured in vitro. Corneas were cultured and exposed to NM as described. Naive corneas or corneas 1 or 3 days after NM exposure were embedded in OCT, frozen, and 8- to 10-μm cryosections made and mounted on slides. Naive corneas were cultured in vitro for times matching the NM-exposed corneas. Slides were stained with (FGF-1) or without (negative control) anti-FGF-1 (Abcam 2E12) followed by donkey anti-mouse <t>IgG</t> (DyLight 549) as secondary antibody (red) and counterstained with DAPI (blue).
    Goat Anti Mouse Immunoglobulin G, supplied by Thermo Fisher, used in various techniques. Bioz Stars score: 86/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/goat anti mouse immunoglobulin g/product/Thermo Fisher
    Average 86 stars, based on 1 article reviews
    Price from $9.99 to $1999.99
    goat anti mouse immunoglobulin g - by Bioz Stars, 2021-06
    86/100 stars
      Buy from Supplier

    86
    Thermo Fisher alexa fluor 488 goat anti mouse immunoglobulin g
    Analysis of the region of JEV NS5 that determines its IFN antagonist activity. (A) Schematic diagram of the full-length and truncated NS5-Flag-tagged fusion constructs and a summary of their IFN antagonist activities. (B) Protein expression patterns of NS5 deletion constructs. BHK-21 cells transfected with the indicated NS5 constructs for 24 h were harvested and analyzed by Western blotting with anti-Flag antibody. (C) Predicted molecular sizes of NS5 truncated mutants. (D) BHK-21 cells were cotransfected with pStat1-DsRed and various plasmids expressing the full-length or truncated NS5 proteins. At 24 h after transfection, cells were stimulated with IFN-αA/D (2,500 U/ml) for 16 h and then fixed and permeabilized for immunofluorescence assay. NS5 proteins were detected by using anti-Flag antibody and <t>Alexa-Fluor</t> 488 goat anti-mouse antibody (green). The locations of Stat1-DsRed (red) in the Flag-positive cells were observed under a fluorescence microscope. Representative cells from the same field for each experimental group are shown.
    Alexa Fluor 488 Goat Anti Mouse Immunoglobulin G, supplied by Thermo Fisher, used in various techniques. Bioz Stars score: 86/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/alexa fluor 488 goat anti mouse immunoglobulin g/product/Thermo Fisher
    Average 86 stars, based on 1 article reviews
    Price from $9.99 to $1999.99
    alexa fluor 488 goat anti mouse immunoglobulin g - by Bioz Stars, 2021-06
    86/100 stars
      Buy from Supplier

    94
    Thermo Fisher goat anti mouse immunoglobulin g igg
    Analysis of the region of JEV NS5 that determines its IFN antagonist activity. (A) Schematic diagram of the full-length and truncated NS5-Flag-tagged fusion constructs and a summary of their IFN antagonist activities. (B) Protein expression patterns of NS5 deletion constructs. BHK-21 cells transfected with the indicated NS5 constructs for 24 h were harvested and analyzed by Western blotting with anti-Flag antibody. (C) Predicted molecular sizes of NS5 truncated mutants. (D) BHK-21 cells were cotransfected with pStat1-DsRed and various plasmids expressing the full-length or truncated NS5 proteins. At 24 h after transfection, cells were stimulated with IFN-αA/D (2,500 U/ml) for 16 h and then fixed and permeabilized for immunofluorescence assay. NS5 proteins were detected by using anti-Flag antibody and <t>Alexa-Fluor</t> 488 goat anti-mouse antibody (green). The locations of Stat1-DsRed (red) in the Flag-positive cells were observed under a fluorescence microscope. Representative cells from the same field for each experimental group are shown.
    Goat Anti Mouse Immunoglobulin G Igg, supplied by Thermo Fisher, used in various techniques. Bioz Stars score: 94/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/goat anti mouse immunoglobulin g igg/product/Thermo Fisher
    Average 94 stars, based on 1 article reviews
    Price from $9.99 to $1999.99
    goat anti mouse immunoglobulin g igg - by Bioz Stars, 2021-06
    94/100 stars
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    95
    Millipore goat anti mouse igg
    Analysis of the region of JEV NS5 that determines its IFN antagonist activity. (A) Schematic diagram of the full-length and truncated NS5-Flag-tagged fusion constructs and a summary of their IFN antagonist activities. (B) Protein expression patterns of NS5 deletion constructs. BHK-21 cells transfected with the indicated NS5 constructs for 24 h were harvested and analyzed by Western blotting with anti-Flag antibody. (C) Predicted molecular sizes of NS5 truncated mutants. (D) BHK-21 cells were cotransfected with pStat1-DsRed and various plasmids expressing the full-length or truncated NS5 proteins. At 24 h after transfection, cells were stimulated with IFN-αA/D (2,500 U/ml) for 16 h and then fixed and permeabilized for immunofluorescence assay. NS5 proteins were detected by using anti-Flag antibody and <t>Alexa-Fluor</t> 488 goat anti-mouse antibody (green). The locations of Stat1-DsRed (red) in the Flag-positive cells were observed under a fluorescence microscope. Representative cells from the same field for each experimental group are shown.
    Goat Anti Mouse Igg, supplied by Millipore, used in various techniques. Bioz Stars score: 95/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/goat anti mouse igg/product/Millipore
    Average 95 stars, based on 1 article reviews
    Price from $9.99 to $1999.99
    goat anti mouse igg - by Bioz Stars, 2021-06
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    Goat anti Mouse IgG antibody
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    Goat anti Mouse IgG IgA IgM H L secondary antibody
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    Goat anti Mouse IgG IgM H L secondary antibody
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    Goat anti Mouse IgG secondary antibody biotin
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    Image Search Results


    NM exposure reduces native FGF-1 levels in corneas cultured in vitro. Corneas were cultured and exposed to NM as described. Naive corneas or corneas 1 or 3 days after NM exposure were embedded in OCT, frozen, and 8- to 10-μm cryosections made and mounted on slides. Naive corneas were cultured in vitro for times matching the NM-exposed corneas. Slides were stained with (FGF-1) or without (negative control) anti-FGF-1 (Abcam 2E12) followed by donkey anti-mouse IgG (DyLight 549) as secondary antibody (red) and counterstained with DAPI (blue).

    Journal: Investigative Ophthalmology & Visual Science

    Article Title: An Engineered Human Fibroblast Growth Factor-1 Derivative, TTHX1114, Ameliorates Short-term Corneal Nitrogen Mustard Injury in Rabbit Organ Cultures

    doi: 10.1167/iovs.18-24568

    Figure Lengend Snippet: NM exposure reduces native FGF-1 levels in corneas cultured in vitro. Corneas were cultured and exposed to NM as described. Naive corneas or corneas 1 or 3 days after NM exposure were embedded in OCT, frozen, and 8- to 10-μm cryosections made and mounted on slides. Naive corneas were cultured in vitro for times matching the NM-exposed corneas. Slides were stained with (FGF-1) or without (negative control) anti-FGF-1 (Abcam 2E12) followed by donkey anti-mouse IgG (DyLight 549) as secondary antibody (red) and counterstained with DAPI (blue).

    Article Snippet: Sections were incubated for 1 hour in 5 μg/mL antibody to human ADAM-17 (MAB 9304; R & D Systems, Minneapolis, MN, USA) in PBS with 1.5% NGS at room temperature, washed three times with PBS with 0.05% Tween-20, and incubated for 1 hour with goat anti-mouse immunoglobulin G (IgG)–Alexa Fluor 488 conjugate (Invitrogen) 1:1000 in PBS with 1.5% NGS.

    Techniques: Cell Culture, In Vitro, Staining, Negative Control

    Analysis of the region of JEV NS5 that determines its IFN antagonist activity. (A) Schematic diagram of the full-length and truncated NS5-Flag-tagged fusion constructs and a summary of their IFN antagonist activities. (B) Protein expression patterns of NS5 deletion constructs. BHK-21 cells transfected with the indicated NS5 constructs for 24 h were harvested and analyzed by Western blotting with anti-Flag antibody. (C) Predicted molecular sizes of NS5 truncated mutants. (D) BHK-21 cells were cotransfected with pStat1-DsRed and various plasmids expressing the full-length or truncated NS5 proteins. At 24 h after transfection, cells were stimulated with IFN-αA/D (2,500 U/ml) for 16 h and then fixed and permeabilized for immunofluorescence assay. NS5 proteins were detected by using anti-Flag antibody and Alexa-Fluor 488 goat anti-mouse antibody (green). The locations of Stat1-DsRed (red) in the Flag-positive cells were observed under a fluorescence microscope. Representative cells from the same field for each experimental group are shown.

    Journal: Journal of Virology

    Article Title: Blocking of Interferon-Induced Jak-Stat Signaling by Japanese Encephalitis Virus NS5 through a Protein Tyrosine Phosphatase-Mediated Mechanism

    doi: 10.1128/JVI.02714-05

    Figure Lengend Snippet: Analysis of the region of JEV NS5 that determines its IFN antagonist activity. (A) Schematic diagram of the full-length and truncated NS5-Flag-tagged fusion constructs and a summary of their IFN antagonist activities. (B) Protein expression patterns of NS5 deletion constructs. BHK-21 cells transfected with the indicated NS5 constructs for 24 h were harvested and analyzed by Western blotting with anti-Flag antibody. (C) Predicted molecular sizes of NS5 truncated mutants. (D) BHK-21 cells were cotransfected with pStat1-DsRed and various plasmids expressing the full-length or truncated NS5 proteins. At 24 h after transfection, cells were stimulated with IFN-αA/D (2,500 U/ml) for 16 h and then fixed and permeabilized for immunofluorescence assay. NS5 proteins were detected by using anti-Flag antibody and Alexa-Fluor 488 goat anti-mouse antibody (green). The locations of Stat1-DsRed (red) in the Flag-positive cells were observed under a fluorescence microscope. Representative cells from the same field for each experimental group are shown.

    Article Snippet: The cells were probed with anti-Flag M2 antibody and subsequently with Alexa-Fluor 488 goat anti-mouse immunoglobulin G (Molecular Probes).

    Techniques: Activity Assay, Construct, Expressing, Transfection, Western Blot, Immunofluorescence, Fluorescence, Microscopy

    Inhibition of IFN-stimulated nuclear translocation of Stat1-DsRed by JEV NS5 in BHK-21 cells. BHK-21 cells were cotransfected with plasmids expressing the indicated JEV proteins and pStat1-DsRed. Twenty-four hours after transfection, cells were stimulated with or without IFN-αA/D (2,500 U/ml) for 16 h. Cells were then fixed and permeabilized for immunofluorescence assay. JEV proteins (C, prM, E, NS1, NS2A, NS2B, NS2B3, NS4A, NS4B, and NS5) were detected using a mouse anti-Flag antibody and Alexa-Fluor 488 goat anti-mouse antibody (green). JEV prM-E and NS3 were detected using anti-JEV E and NS3 antibodies, respectively, and then stained with Alexa-Fluor 488 goat anti-mouse antibody (green). Nuclear translocation of Stat1-DsRed (red) was observed under a fluorescence microscope. Representative cells from the same field for each experimental group are shown.

    Journal: Journal of Virology

    Article Title: Blocking of Interferon-Induced Jak-Stat Signaling by Japanese Encephalitis Virus NS5 through a Protein Tyrosine Phosphatase-Mediated Mechanism

    doi: 10.1128/JVI.02714-05

    Figure Lengend Snippet: Inhibition of IFN-stimulated nuclear translocation of Stat1-DsRed by JEV NS5 in BHK-21 cells. BHK-21 cells were cotransfected with plasmids expressing the indicated JEV proteins and pStat1-DsRed. Twenty-four hours after transfection, cells were stimulated with or without IFN-αA/D (2,500 U/ml) for 16 h. Cells were then fixed and permeabilized for immunofluorescence assay. JEV proteins (C, prM, E, NS1, NS2A, NS2B, NS2B3, NS4A, NS4B, and NS5) were detected using a mouse anti-Flag antibody and Alexa-Fluor 488 goat anti-mouse antibody (green). JEV prM-E and NS3 were detected using anti-JEV E and NS3 antibodies, respectively, and then stained with Alexa-Fluor 488 goat anti-mouse antibody (green). Nuclear translocation of Stat1-DsRed (red) was observed under a fluorescence microscope. Representative cells from the same field for each experimental group are shown.

    Article Snippet: The cells were probed with anti-Flag M2 antibody and subsequently with Alexa-Fluor 488 goat anti-mouse immunoglobulin G (Molecular Probes).

    Techniques: Inhibition, Translocation Assay, Expressing, Transfection, Immunofluorescence, Staining, Fluorescence, Microscopy