Journal: Oncology Reports
Article Title: Upregulation of GNL3 expression promotes colon cancer cell proliferation, migration, invasion and epithelial-mesenchymal transition via the Wnt/β-catenin signaling pathway
Figure Lengend Snippet: Effects of GNL3 knockdown and overexpression on the levels of the EMT-related markers, β-catenin and GNL3 in vitro . (A and B) In HCT-116 cells, GNL3 knockdown (GNL3 shRNA) increased E-cadherin levels and reduced N-cadherin, vimentin, nuclear β-catenin (β-catenin-N) and GNL3 levels compared with controls (Con). (A and C) In HT-29 cells, GNL3 overexpression (GNL3) increased the N-cadherin, vimentin, β-catenin-N and GNL3 levels and reduced the E-cadherin levels compared with the negative controls (NC). **P
Article Snippet: GNL3 knockdown plasmids were purchased from Sigma-Aldrich (St. Louis, MO, USA; TRCN0000293740) and GNL3 expression plasmids were purchased from Sino Biological Inc. (Beijing, China; HG12415-UT).
Techniques: Over Expression, In Vitro, shRNA