Journal: Scientific Reports
Article Title: Cytokines regulate complement receptor immunoglobulin expression and phagocytosis of Candida albicans in human macrophages: A control point in anti-microbial immunity
Figure Lengend Snippet: Effects of cytokines on CRIg expression in matured macrophages (MDM). In these studies MDM were prepared by culturing human monocytes for 3 days. MDM from 3 day cultures were treated with LT-α ( a ) (0, 5, 10, 20 and 40 ng/ml) or IFN-γ ( b ) (0, 5, 20 and 40 ng/ml) or IL-4 ( c ) (0, 1, 3, 5, 10 and 40 ng/ml) or IL-13 ( d ) (0, 5, 10, 20 and 40 ng/ml), TGF-β1 ( e ) (0, 2, 5 and 15 ng/ml) or ( f ) IL-10 (0, 5, 10, 20 and 40 ng/ml) or M-CSF/GM-CSF ( g , h ) (0, 5, 10, 20 and 40 ng/ml) or dexamethasone ( i ) (0, 10, 30 and 50 ng/ml) for 24 h and then CRIg mRNA levels relative to GAPDH mRNA were assessed by qPCR. Data are expressed as fold-change over GAPDH-normalised CRIg mRNA in the absence of cytokine set as 1. Data are presented as means ± SD of three experiments, each conducted with cells from different individuals, *p
Article Snippet: Cytokines and cell culture reagents Recombinant granulocyte-macrophage colony stimulating factor (GM-CSF), macrophage (M)-CSF, interleukin (IL)-1β, IL-6, IL-4, IL-10, IL-13, interferon (IFN)-γ, lymphotoxin (LT)-α, tumor necrosis factor (TNF), M-CSF and GM-CSF were purchased from ProSpec-Tany Technogene (Rehovot, Israel), transforming growth factor (TGF)-β1 from R & D Systems (Minneapolis, MN), and dexamethasone was purchased from Sigma-Aldrich (St. Louis, MO).
Techniques: Expressing, Real-time Polymerase Chain Reaction