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  • 99
    Millipore glyceraldehyde 3 phosphate dehydrogenase gapdh
    Effects of ethanol and androgenization on profibrotic gene expression. Quantitative Real-Time PCR analysis of Interleukin-6 (IL-6) (black bar) and collagen Type 3 (COL3α1) (striped bar) gene expression in control (Ctrl), androgenized (Andro) and Andro + estrogen (Andro + E2) animals maintained on control Lieber-DeCarli (C-LDC) or ethanol-LDC (E-LDC) diet. Gene expression was normalized to <t>glyceraldehyde-3-phosphate</t> dehydrogenase <t>(GAPDH).</t> Data are presented as mean values ± SEM. *P
    Glyceraldehyde 3 Phosphate Dehydrogenase Gapdh, supplied by Millipore, used in various techniques. Bioz Stars score: 99/100, based on 1528 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    Proteintech glyceraldehyde 3 phosphate dehydrogenase gapdh
    Effects of DACT1 on β-catenin in myocardial cells. Effect of DACT1 overexpression on β-catenin concentration A) and distribution B) in H9C2 and HL-1 cells. C) Correlation between DACT1 and β-catenin expression in the myocardial tissues of patients with valvular heart disease. D) Colocation of DACT1 and β-catenin in the myocardial cells. H9C2 and HL-1 cells were overexpressed with the two isoforms of the DACT1 coding sequence, and then immunofluorescence was used to detect the DACT1 (DACT1-V1 or DACT1-V2) (OriGene) and β-catenin (CST) location. The secondary antibody Alexa Fluor 647 was used for DACT1 detection and Alexa Fluor 568 for β-catenin. The ZEN (Zeiss) software was used for further analysis. Alexa Fluor 647 was represented by the green color and Alexa Fluor 568 by the red color, while channel 488 was closed to avoid interference. DACT1=dishevelled binding antagonist of beta catenin 1; DAPI=2-(4-amidinophenyl)-6-indolecarbamidine dihydrochloride; <t>GAPDH=glyceraldehyde-3-phosphate</t> dehydrogenase
    Glyceraldehyde 3 Phosphate Dehydrogenase Gapdh, supplied by Proteintech, used in various techniques. Bioz Stars score: 92/100, based on 188 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    Merck KGaA glyceraldehyde 3 phosphate dehydrogenase gapdh
    Effects of DACT1 on β-catenin in myocardial cells. Effect of DACT1 overexpression on β-catenin concentration A) and distribution B) in H9C2 and HL-1 cells. C) Correlation between DACT1 and β-catenin expression in the myocardial tissues of patients with valvular heart disease. D) Colocation of DACT1 and β-catenin in the myocardial cells. H9C2 and HL-1 cells were overexpressed with the two isoforms of the DACT1 coding sequence, and then immunofluorescence was used to detect the DACT1 (DACT1-V1 or DACT1-V2) (OriGene) and β-catenin (CST) location. The secondary antibody Alexa Fluor 647 was used for DACT1 detection and Alexa Fluor 568 for β-catenin. The ZEN (Zeiss) software was used for further analysis. Alexa Fluor 647 was represented by the green color and Alexa Fluor 568 by the red color, while channel 488 was closed to avoid interference. DACT1=dishevelled binding antagonist of beta catenin 1; DAPI=2-(4-amidinophenyl)-6-indolecarbamidine dihydrochloride; <t>GAPDH=glyceraldehyde-3-phosphate</t> dehydrogenase
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    Thermo Fisher glyceraldehyde 3 phosphate dehydrogenase gapdh
    Comparison of RNase expression and HIV inhibition in T h 17-polarized cells with those in T h 0-polarized cells. (A) Representative flow cytometry plots depicting IL-17 expression among T h 0-polarized, CCR6 − cells and T h 17-polarized, CCR6 + cells. SSC, side scatter. (B) Box plot showing differential gene expression between T h 0- and T h 17-polarized cells. Total RNA from T h 0- or T h 17-polarized cells was analyzed by using microarrays. Relative levels of gene expression in T h 17-polarized cells and T h 0-polarized cells are shown. (C) Thirty micrograms of total protein from cell lysates of CCR6 − , T h 0-polarized and CCR6 + , T h 17-polarized CD4 T cells from three donors was denatured and separated according to size by SDS-PAGE, transferred to a PVDF membrane, and then probed for the indicated RNase expression with anti-RNase antibodies. The loading control was glyceraldehyde 3-phosphate dehydrogenase <t>(GAPDH).</t> (D) T h 0- and T h 17-polarized CD4 T cells were infected with HIV IIIB , washed, and then resuspended in medium containing the indicated RNases. Percent inhibition was calculated from the percentage of p24 + cells, as measured by flow cytometry.
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    Santa Cruz Biotechnology glyceraldehyde 3 phosphate dehydrogenase gapdh
    Atorvastatin effect on the expression of smooth muscle actin. <t>GAPDH:</t> <t>Glyceraldehyde-3-phosphate</t> dehydrogenase; SMA: Smooth muscle actin.
    Glyceraldehyde 3 Phosphate Dehydrogenase Gapdh, supplied by Santa Cruz Biotechnology, used in various techniques. Bioz Stars score: 93/100, based on 2247 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    Sangon Biotech glyceraldehyde 3 phosphate dehydrogenase gapdh
    Efficient differentiation of mesenchymal stem cells (MSCs) from human induced pluripotent stem cells (iPSCs) and characterization of iMSCs-Exo. (A) Phase-contrast image of long-term cultured iPSC clone before differentiation (i), intermediate phase of induced iPSCs (ii), and iMSCs (iii). (B) Quantitative reverse-transcriptase polymerase chain reaction analysis of the expression level of pluripotent genes (Nanog, Oct4, and Msx1) in iPSCs during differentiation (i) and in iPSCs and iMSCs (ii). (C) Flow cytometric analysis of mesenchymal positive markers, such as CD29, CD44, CD73, CD90, CD105, and CD146, and negative markers, such as CD34, CD45, CD133, and HLA-DR. Black histograms represent the isotype controls, and the red solid peak represents the marker indicated. (D) Multi-differentiation potential of iMSCs. (i) Alizarin Red staining of osteogenic mineralization (day 14). (ii) Oil Red O staining of small lipid droplets (day 21). (iii) Toluidine Blue staining of cartilaginous extracellular matrix (day 21). (E) Morphology of iMSCs-Exo under transmission electron microscopy. (F) Western blotting analysis of exosomal CD63, CD81, and CD9 protein in iMSCs and iMSCs-Exo. The culture medium served as the control. <t>GAPDH,</t> glyceraldehyde 3-phosphate dehydrogenase; iMSC, induced pluripotent stem cell-derived mesenchymal stem cell; iMSCs-Exo, exosomes derived from induced pluripotent stem cell-derived mesenchymal stem cells.
    Glyceraldehyde 3 Phosphate Dehydrogenase Gapdh, supplied by Sangon Biotech, used in various techniques. Bioz Stars score: 92/100, based on 142 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    Active Motif glyceraldehyde 3 phosphate dehydrogenase gapdh
    Efficient differentiation of mesenchymal stem cells (MSCs) from human induced pluripotent stem cells (iPSCs) and characterization of iMSCs-Exo. (A) Phase-contrast image of long-term cultured iPSC clone before differentiation (i), intermediate phase of induced iPSCs (ii), and iMSCs (iii). (B) Quantitative reverse-transcriptase polymerase chain reaction analysis of the expression level of pluripotent genes (Nanog, Oct4, and Msx1) in iPSCs during differentiation (i) and in iPSCs and iMSCs (ii). (C) Flow cytometric analysis of mesenchymal positive markers, such as CD29, CD44, CD73, CD90, CD105, and CD146, and negative markers, such as CD34, CD45, CD133, and HLA-DR. Black histograms represent the isotype controls, and the red solid peak represents the marker indicated. (D) Multi-differentiation potential of iMSCs. (i) Alizarin Red staining of osteogenic mineralization (day 14). (ii) Oil Red O staining of small lipid droplets (day 21). (iii) Toluidine Blue staining of cartilaginous extracellular matrix (day 21). (E) Morphology of iMSCs-Exo under transmission electron microscopy. (F) Western blotting analysis of exosomal CD63, CD81, and CD9 protein in iMSCs and iMSCs-Exo. The culture medium served as the control. <t>GAPDH,</t> glyceraldehyde 3-phosphate dehydrogenase; iMSC, induced pluripotent stem cell-derived mesenchymal stem cell; iMSCs-Exo, exosomes derived from induced pluripotent stem cell-derived mesenchymal stem cells.
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    Cell Signaling Technology Inc glyceraldehyde 3 phosphate dehydrogenase gapdh
    P2X7 and EGF Receptor Stimulation Regulates the Expression of DUSP6 in Cerebellar Granule Neurons and Astrocytes ) for different times and DUSP6 and <t>Glyceraldehyde-3-phosphate</t> dehydrogenase <t>(GAPDH)</t> were detected in the total lysates by immunoblotting. (A) Immunoblots of a representative time-course experiment for each effector. (B) Cells were incubated with or without the effectors, 300 μM BzATP or 100 ng/mL EGF, for 30 min, fixed and the presence of DUSP6 was detected by immunocytochemistry. Representative immunofluorescence images are shown. Scale bars represent 50 μm. (C) The diagrams represent the quantification of the time courses obtained by normalization to the corresponding GAPDH levels. The data are represented as the means ± SEM from three independent experiments performed on different cultures. *** p
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    SABiosciences glyceraldehyde 3 phosphate dehydrogenase gapdh
    P2X7 and EGF Receptor Stimulation Regulates the Expression of DUSP6 in Cerebellar Granule Neurons and Astrocytes ) for different times and DUSP6 and <t>Glyceraldehyde-3-phosphate</t> dehydrogenase <t>(GAPDH)</t> were detected in the total lysates by immunoblotting. (A) Immunoblots of a representative time-course experiment for each effector. (B) Cells were incubated with or without the effectors, 300 μM BzATP or 100 ng/mL EGF, for 30 min, fixed and the presence of DUSP6 was detected by immunocytochemistry. Representative immunofluorescence images are shown. Scale bars represent 50 μm. (C) The diagrams represent the quantification of the time courses obtained by normalization to the corresponding GAPDH levels. The data are represented as the means ± SEM from three independent experiments performed on different cultures. *** p
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    GeneTex glyceraldehyde 3 phosphate dehydrogenase gapdh
    Effects of increases in dietary LA and dietary OXLAMs on 4-HNE in mouse cerebral cortex. N = 8 tissue samples per group. Immunoblot analysis of cerebral cortex protein lysates for the detection of 4-HNE-modified proteins. Glyceraldehyde 3-phos-phate dehydrogenase <t>(GAPDH)</t> was used as a loading control. Densitometric analysis was performed on background-subtracted blots and normalized to GAPDH. The Low LA group was used as a reference control and set to 1.
    Glyceraldehyde 3 Phosphate Dehydrogenase Gapdh, supplied by GeneTex, used in various techniques. Bioz Stars score: 93/100, based on 95 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    Bioworld Antibodies glyceraldehyde 3 phosphate dehydrogenase gapdh
    Effects of increases in dietary LA and dietary OXLAMs on 4-HNE in mouse cerebral cortex. N = 8 tissue samples per group. Immunoblot analysis of cerebral cortex protein lysates for the detection of 4-HNE-modified proteins. Glyceraldehyde 3-phos-phate dehydrogenase <t>(GAPDH)</t> was used as a loading control. Densitometric analysis was performed on background-subtracted blots and normalized to GAPDH. The Low LA group was used as a reference control and set to 1.
    Glyceraldehyde 3 Phosphate Dehydrogenase Gapdh, supplied by Bioworld Antibodies, used in various techniques. Bioz Stars score: 90/100, based on 56 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    Trinity Biotech glyceraldehyde 3 phosphate dehydrogenase gapdh
    Effects of increases in dietary LA and dietary OXLAMs on 4-HNE in mouse cerebral cortex. N = 8 tissue samples per group. Immunoblot analysis of cerebral cortex protein lysates for the detection of 4-HNE-modified proteins. Glyceraldehyde 3-phos-phate dehydrogenase <t>(GAPDH)</t> was used as a loading control. Densitometric analysis was performed on background-subtracted blots and normalized to GAPDH. The Low LA group was used as a reference control and set to 1.
    Glyceraldehyde 3 Phosphate Dehydrogenase Gapdh, supplied by Trinity Biotech, used in various techniques. Bioz Stars score: 92/100, based on 75 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    R&D Systems glyceraldehyde 3 phosphate dehydrogenase gapdh
    Effects of increases in dietary LA and dietary OXLAMs on 4-HNE in mouse cerebral cortex. N = 8 tissue samples per group. Immunoblot analysis of cerebral cortex protein lysates for the detection of 4-HNE-modified proteins. Glyceraldehyde 3-phos-phate dehydrogenase <t>(GAPDH)</t> was used as a loading control. Densitometric analysis was performed on background-subtracted blots and normalized to GAPDH. The Low LA group was used as a reference control and set to 1.
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    rdi research diagnostics glyceraldehyde 3 phosphate dehydrogenase gapdh
    Effects of increases in dietary LA and dietary OXLAMs on 4-HNE in mouse cerebral cortex. N = 8 tissue samples per group. Immunoblot analysis of cerebral cortex protein lysates for the detection of 4-HNE-modified proteins. Glyceraldehyde 3-phos-phate dehydrogenase <t>(GAPDH)</t> was used as a loading control. Densitometric analysis was performed on background-subtracted blots and normalized to GAPDH. The Low LA group was used as a reference control and set to 1.
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    Bioss glyceraldehyde 3 phosphate dehydrogenase gapdh
    Effects of increases in dietary LA and dietary OXLAMs on 4-HNE in mouse cerebral cortex. N = 8 tissue samples per group. Immunoblot analysis of cerebral cortex protein lysates for the detection of 4-HNE-modified proteins. Glyceraldehyde 3-phos-phate dehydrogenase <t>(GAPDH)</t> was used as a loading control. Densitometric analysis was performed on background-subtracted blots and normalized to GAPDH. The Low LA group was used as a reference control and set to 1.
    Glyceraldehyde 3 Phosphate Dehydrogenase Gapdh, supplied by Bioss, used in various techniques. Bioz Stars score: 90/100, based on 15 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    ABclonal glyceraldehyde 3 phosphate dehydrogenase gapdh
    Effect of Phosphoglycerate Kinase 1 (PGK1) on the proliferation of Hepatocellular Carcinoma (HCC) cells. ( A ) Endogenous expression of PGK1 in nomal and five HCC cell lines by Western blotting. Glyceraldehyde 3-phosphate dehydrogenase <t>(GAPDH)</t> was used as internal control. ( B ) Transfection efficiency of PGK1 overexpress and knockdown in HCC. GAPDH was used as internal control. ( C ) Effect of PGK1 on proliferation of SNU182 and JHH5 cells by cell counting kit-8 (CCK8) assay. ( D ) Effect of PGK1 knockdown on proliferation of SNU449 and HCCLM3 cells by CCK8 assay. ( E ) Effect of PGK1 on proliferation of SNU182 and JHH5 cells by plate colony formation assay. ( F ) Effect of PGK1 knockdown on proliferation of SNU449 and HCCLM3 cells by plate colony formation assay. ** p
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    TaKaRa glyceraldehyde 3 phosphate dehydrogenase gapdh
    Expression and induction of IL-17R and IL-17RB in IL-17-stimulated FLS from six RA patients. (a) IL-17 dose-dependent changes in the levels of IL-17R and IL-17RB mRNAs. Three independent RA FLS cell lines were stimulated with various amounts of recombinant IL-17 (0 to 20 ng/ml), and subsequent changes in the mRNA levels of IL-17R and IL-17RB were assessed by RT-PCR at 6 hours after the onset of in vitro culture. The relative intensity of each PCR band was normalized against that of <t>GAPDH.</t> Values are the fold increase from the unstimulated cell in each FLS line. (b) Time-dependent changes in the level of IL-17R and IL-17RB mRNAs. Three independent RA FLS cell lines were stimulated with recombinant IL-17, and subsequent changes in the mRNA level of IL-17R and IL-17RB were assessed by RT-PCR 0, 1, 3, 6, 9, and 24 hours after the start of in vitro culture. The relative intensity of each PCR band was normalized against that of GAPDH. Values are the fold increase from the 0 hour measurement in each FLS line. FLS, fibroblast-like synoviocytes; GAPDH, <t>glyceraldehyde-3-phosphate</t> dehydrogenase; IL-17R, IL-17 receptor; IL-17RB, IL-17 receptor B; RA, rheumatoid arthritis.
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    Stratagene glyceraldehyde 3 phosphate dehydrogenase gapdh
    Expression and induction of IL-17R and IL-17RB in IL-17-stimulated FLS from six RA patients. (a) IL-17 dose-dependent changes in the levels of IL-17R and IL-17RB mRNAs. Three independent RA FLS cell lines were stimulated with various amounts of recombinant IL-17 (0 to 20 ng/ml), and subsequent changes in the mRNA levels of IL-17R and IL-17RB were assessed by RT-PCR at 6 hours after the onset of in vitro culture. The relative intensity of each PCR band was normalized against that of <t>GAPDH.</t> Values are the fold increase from the unstimulated cell in each FLS line. (b) Time-dependent changes in the level of IL-17R and IL-17RB mRNAs. Three independent RA FLS cell lines were stimulated with recombinant IL-17, and subsequent changes in the mRNA level of IL-17R and IL-17RB were assessed by RT-PCR 0, 1, 3, 6, 9, and 24 hours after the start of in vitro culture. The relative intensity of each PCR band was normalized against that of GAPDH. Values are the fold increase from the 0 hour measurement in each FLS line. FLS, fibroblast-like synoviocytes; GAPDH, <t>glyceraldehyde-3-phosphate</t> dehydrogenase; IL-17R, IL-17 receptor; IL-17RB, IL-17 receptor B; RA, rheumatoid arthritis.
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    Abmart glyceraldehyde 3 phosphate dehydrogenase gapdh
    Expression and induction of IL-17R and IL-17RB in IL-17-stimulated FLS from six RA patients. (a) IL-17 dose-dependent changes in the levels of IL-17R and IL-17RB mRNAs. Three independent RA FLS cell lines were stimulated with various amounts of recombinant IL-17 (0 to 20 ng/ml), and subsequent changes in the mRNA levels of IL-17R and IL-17RB were assessed by RT-PCR at 6 hours after the onset of in vitro culture. The relative intensity of each PCR band was normalized against that of <t>GAPDH.</t> Values are the fold increase from the unstimulated cell in each FLS line. (b) Time-dependent changes in the level of IL-17R and IL-17RB mRNAs. Three independent RA FLS cell lines were stimulated with recombinant IL-17, and subsequent changes in the mRNA level of IL-17R and IL-17RB were assessed by RT-PCR 0, 1, 3, 6, 9, and 24 hours after the start of in vitro culture. The relative intensity of each PCR band was normalized against that of GAPDH. Values are the fold increase from the 0 hour measurement in each FLS line. FLS, fibroblast-like synoviocytes; GAPDH, <t>glyceraldehyde-3-phosphate</t> dehydrogenase; IL-17R, IL-17 receptor; IL-17RB, IL-17 receptor B; RA, rheumatoid arthritis.
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    Bio-Rad glyceraldehyde 3 phosphate dehydrogenase gapdh
    GROβ mRNA levels in LSCC tissues were significantly increased compared with corresponding non-cancerous tissues, as determined by reverse transcription-quantitative polymerase chain reaction, with data normalized to <t>GAPDH</t> mRNA levels (P=0.009). GROβ, growth-related gene product β; LSCC, laryngeal squamous cell carcinoma; GADPH, glyceraldehyde 3-phosphate dehydrogenase.
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    Millipore human glyceraldehyde 3 phosphate dehydrogenase gapdh
    GROβ mRNA levels in LSCC tissues were significantly increased compared with corresponding non-cancerous tissues, as determined by reverse transcription-quantitative polymerase chain reaction, with data normalized to <t>GAPDH</t> mRNA levels (P=0.009). GROβ, growth-related gene product β; LSCC, laryngeal squamous cell carcinoma; GADPH, glyceraldehyde 3-phosphate dehydrogenase.
    Human Glyceraldehyde 3 Phosphate Dehydrogenase Gapdh, supplied by Millipore, used in various techniques. Bioz Stars score: 99/100, based on 6 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    Abfrontier glyceraldehyde 3 phosphate dehydrogenase gapdh
    The effect of esculetin on the expression of cell cycle arrest- and apoptosis-related proteins in human malignant melanoma. (A) The G361 cells were incubated with esculetin (20, 40, and 80 μg/mL) or without for 48 hours. The cell lysates were determined using Western blot analysis with antibodies against p27, p21, and cyclin D1. Equal loading protein was confirmed using <t>glyceraldehyde-3-phosphate</t> de-hydrogenase <t>(GAPDH).</t> (B) The G361 cells were treated with esculetin (0, 20, 40, and 80 μg/mL) for 48 hours. The cell lysates were then determined via Western blot analysis with antibodies against procaspase 3, active caspase 3, Bax, and PARP. Equal loading protein was confirmed using GAPDH. The values were measured using Image J densitometry, and the data were expressed as a representative of two or three independent experiments.
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    Valiant glyceraldehyde 3 phosphate dehydrogenase gapdh
    The effect of esculetin on the expression of cell cycle arrest- and apoptosis-related proteins in human malignant melanoma. (A) The G361 cells were incubated with esculetin (20, 40, and 80 μg/mL) or without for 48 hours. The cell lysates were determined using Western blot analysis with antibodies against p27, p21, and cyclin D1. Equal loading protein was confirmed using <t>glyceraldehyde-3-phosphate</t> de-hydrogenase <t>(GAPDH).</t> (B) The G361 cells were treated with esculetin (0, 20, 40, and 80 μg/mL) for 48 hours. The cell lysates were then determined via Western blot analysis with antibodies against procaspase 3, active caspase 3, Bax, and PARP. Equal loading protein was confirmed using GAPDH. The values were measured using Image J densitometry, and the data were expressed as a representative of two or three independent experiments.
    Glyceraldehyde 3 Phosphate Dehydrogenase Gapdh, supplied by Valiant, used in various techniques. Bioz Stars score: 92/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    Millipore chloroplast gapdh
    The effect of esculetin on the expression of cell cycle arrest- and apoptosis-related proteins in human malignant melanoma. (A) The G361 cells were incubated with esculetin (20, 40, and 80 μg/mL) or without for 48 hours. The cell lysates were determined using Western blot analysis with antibodies against p27, p21, and cyclin D1. Equal loading protein was confirmed using <t>glyceraldehyde-3-phosphate</t> de-hydrogenase <t>(GAPDH).</t> (B) The G361 cells were treated with esculetin (0, 20, 40, and 80 μg/mL) for 48 hours. The cell lysates were then determined via Western blot analysis with antibodies against procaspase 3, active caspase 3, Bax, and PARP. Equal loading protein was confirmed using GAPDH. The values were measured using Image J densitometry, and the data were expressed as a representative of two or three independent experiments.
    Chloroplast Gapdh, supplied by Millipore, used in various techniques. Bioz Stars score: 99/100, based on 3 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    Agilent technologies glyceraldehyde 3 phosphate dehydrogenase gapdh
    The effect of esculetin on the expression of cell cycle arrest- and apoptosis-related proteins in human malignant melanoma. (A) The G361 cells were incubated with esculetin (20, 40, and 80 μg/mL) or without for 48 hours. The cell lysates were determined using Western blot analysis with antibodies against p27, p21, and cyclin D1. Equal loading protein was confirmed using <t>glyceraldehyde-3-phosphate</t> de-hydrogenase <t>(GAPDH).</t> (B) The G361 cells were treated with esculetin (0, 20, 40, and 80 μg/mL) for 48 hours. The cell lysates were then determined via Western blot analysis with antibodies against procaspase 3, active caspase 3, Bax, and PARP. Equal loading protein was confirmed using GAPDH. The values were measured using Image J densitometry, and the data were expressed as a representative of two or three independent experiments.
    Glyceraldehyde 3 Phosphate Dehydrogenase Gapdh, supplied by Agilent technologies, used in various techniques. Bioz Stars score: 92/100, based on 64 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    Image Search Results


    Effects of ethanol and androgenization on profibrotic gene expression. Quantitative Real-Time PCR analysis of Interleukin-6 (IL-6) (black bar) and collagen Type 3 (COL3α1) (striped bar) gene expression in control (Ctrl), androgenized (Andro) and Andro + estrogen (Andro + E2) animals maintained on control Lieber-DeCarli (C-LDC) or ethanol-LDC (E-LDC) diet. Gene expression was normalized to glyceraldehyde-3-phosphate dehydrogenase (GAPDH). Data are presented as mean values ± SEM. *P

    Journal: PLoS ONE

    Article Title: Neonatal Androgenization Exacerbates Alcohol-Induced Liver Injury in Adult Rats, an Effect Abrogated by Estrogen

    doi: 10.1371/journal.pone.0029463

    Figure Lengend Snippet: Effects of ethanol and androgenization on profibrotic gene expression. Quantitative Real-Time PCR analysis of Interleukin-6 (IL-6) (black bar) and collagen Type 3 (COL3α1) (striped bar) gene expression in control (Ctrl), androgenized (Andro) and Andro + estrogen (Andro + E2) animals maintained on control Lieber-DeCarli (C-LDC) or ethanol-LDC (E-LDC) diet. Gene expression was normalized to glyceraldehyde-3-phosphate dehydrogenase (GAPDH). Data are presented as mean values ± SEM. *P

    Article Snippet: For Western blot analyses, antibodies against cytochrome P450 2E1 (CYP2E1), cytochrome P450 1A2 (CYP1A2) and glyceraldehyde-3-phosphate dehydrogenase (GAPDH) were purchased from Millipore (Billerica, MA).

    Techniques: Expressing, Real-time Polymerase Chain Reaction

    Effects of DACT1 on β-catenin in myocardial cells. Effect of DACT1 overexpression on β-catenin concentration A) and distribution B) in H9C2 and HL-1 cells. C) Correlation between DACT1 and β-catenin expression in the myocardial tissues of patients with valvular heart disease. D) Colocation of DACT1 and β-catenin in the myocardial cells. H9C2 and HL-1 cells were overexpressed with the two isoforms of the DACT1 coding sequence, and then immunofluorescence was used to detect the DACT1 (DACT1-V1 or DACT1-V2) (OriGene) and β-catenin (CST) location. The secondary antibody Alexa Fluor 647 was used for DACT1 detection and Alexa Fluor 568 for β-catenin. The ZEN (Zeiss) software was used for further analysis. Alexa Fluor 647 was represented by the green color and Alexa Fluor 568 by the red color, while channel 488 was closed to avoid interference. DACT1=dishevelled binding antagonist of beta catenin 1; DAPI=2-(4-amidinophenyl)-6-indolecarbamidine dihydrochloride; GAPDH=glyceraldehyde-3-phosphate dehydrogenase

    Journal: Brazilian Journal of Cardiovascular Surgery

    Article Title: DACT1 Involvement in the Cytoskeletal Arrangement of Cardiomyocytes in Atrial Fibrillation by Regulating Cx43

    doi: 10.21470/1678-9741-2019-0033

    Figure Lengend Snippet: Effects of DACT1 on β-catenin in myocardial cells. Effect of DACT1 overexpression on β-catenin concentration A) and distribution B) in H9C2 and HL-1 cells. C) Correlation between DACT1 and β-catenin expression in the myocardial tissues of patients with valvular heart disease. D) Colocation of DACT1 and β-catenin in the myocardial cells. H9C2 and HL-1 cells were overexpressed with the two isoforms of the DACT1 coding sequence, and then immunofluorescence was used to detect the DACT1 (DACT1-V1 or DACT1-V2) (OriGene) and β-catenin (CST) location. The secondary antibody Alexa Fluor 647 was used for DACT1 detection and Alexa Fluor 568 for β-catenin. The ZEN (Zeiss) software was used for further analysis. Alexa Fluor 647 was represented by the green color and Alexa Fluor 568 by the red color, while channel 488 was closed to avoid interference. DACT1=dishevelled binding antagonist of beta catenin 1; DAPI=2-(4-amidinophenyl)-6-indolecarbamidine dihydrochloride; GAPDH=glyceraldehyde-3-phosphate dehydrogenase

    Article Snippet: Glyceraldehyde-3-phosphate dehydrogenase (GAPDH) (Proteintech) or β-actin (Wuhan GoodBio Tech.

    Techniques: Over Expression, Concentration Assay, Expressing, Sequencing, Immunofluorescence, Software, Binding Assay

    The effect of DACT1 on Cx43 in myocardial cells. The effect of DACT1 overexpression on Cx43 concentration A) and distribution B) in H9C2 and HL-1 cells. C) Cx43 expression in the myocardial tissues of patients with valvular heart disease. DACT1=dishevelled binding antagonist of beta catenin 1; GAPDH=glyceraldehyde-3-phosphate dehydrogenase

    Journal: Brazilian Journal of Cardiovascular Surgery

    Article Title: DACT1 Involvement in the Cytoskeletal Arrangement of Cardiomyocytes in Atrial Fibrillation by Regulating Cx43

    doi: 10.21470/1678-9741-2019-0033

    Figure Lengend Snippet: The effect of DACT1 on Cx43 in myocardial cells. The effect of DACT1 overexpression on Cx43 concentration A) and distribution B) in H9C2 and HL-1 cells. C) Cx43 expression in the myocardial tissues of patients with valvular heart disease. DACT1=dishevelled binding antagonist of beta catenin 1; GAPDH=glyceraldehyde-3-phosphate dehydrogenase

    Article Snippet: Glyceraldehyde-3-phosphate dehydrogenase (GAPDH) (Proteintech) or β-actin (Wuhan GoodBio Tech.

    Techniques: Over Expression, Concentration Assay, Expressing, Binding Assay

    Comparison of RNase expression and HIV inhibition in T h 17-polarized cells with those in T h 0-polarized cells. (A) Representative flow cytometry plots depicting IL-17 expression among T h 0-polarized, CCR6 − cells and T h 17-polarized, CCR6 + cells. SSC, side scatter. (B) Box plot showing differential gene expression between T h 0- and T h 17-polarized cells. Total RNA from T h 0- or T h 17-polarized cells was analyzed by using microarrays. Relative levels of gene expression in T h 17-polarized cells and T h 0-polarized cells are shown. (C) Thirty micrograms of total protein from cell lysates of CCR6 − , T h 0-polarized and CCR6 + , T h 17-polarized CD4 T cells from three donors was denatured and separated according to size by SDS-PAGE, transferred to a PVDF membrane, and then probed for the indicated RNase expression with anti-RNase antibodies. The loading control was glyceraldehyde 3-phosphate dehydrogenase (GAPDH). (D) T h 0- and T h 17-polarized CD4 T cells were infected with HIV IIIB , washed, and then resuspended in medium containing the indicated RNases. Percent inhibition was calculated from the percentage of p24 + cells, as measured by flow cytometry.

    Journal: Journal of Virology

    Article Title: Human Th17 Cells Lack HIV-Inhibitory RNases and Are Highly Permissive to Productive HIV Infection

    doi: 10.1128/JVI.02869-15

    Figure Lengend Snippet: Comparison of RNase expression and HIV inhibition in T h 17-polarized cells with those in T h 0-polarized cells. (A) Representative flow cytometry plots depicting IL-17 expression among T h 0-polarized, CCR6 − cells and T h 17-polarized, CCR6 + cells. SSC, side scatter. (B) Box plot showing differential gene expression between T h 0- and T h 17-polarized cells. Total RNA from T h 0- or T h 17-polarized cells was analyzed by using microarrays. Relative levels of gene expression in T h 17-polarized cells and T h 0-polarized cells are shown. (C) Thirty micrograms of total protein from cell lysates of CCR6 − , T h 0-polarized and CCR6 + , T h 17-polarized CD4 T cells from three donors was denatured and separated according to size by SDS-PAGE, transferred to a PVDF membrane, and then probed for the indicated RNase expression with anti-RNase antibodies. The loading control was glyceraldehyde 3-phosphate dehydrogenase (GAPDH). (D) T h 0- and T h 17-polarized CD4 T cells were infected with HIV IIIB , washed, and then resuspended in medium containing the indicated RNases. Percent inhibition was calculated from the percentage of p24 + cells, as measured by flow cytometry.

    Article Snippet: As a loading control, blots were also probed with an HRP-conjugated antibody to glyceraldehyde 3-phosphate dehydrogenase (GAPDH) (ThermoFisher Scientific Inc., Rockville, MD).

    Techniques: Expressing, Inhibition, Flow Cytometry, Cytometry, SDS Page, Infection

    Atorvastatin effect on the expression of smooth muscle actin. GAPDH: Glyceraldehyde-3-phosphate dehydrogenase; SMA: Smooth muscle actin.

    Journal: World Journal of Gastroenterology : WJG

    Article Title: Atorvastatin and rosuvastatin do not prevent thioacetamide induced liver cirrhosis in rats

    doi: 10.3748/wjg.v19.i2.241

    Figure Lengend Snippet: Atorvastatin effect on the expression of smooth muscle actin. GAPDH: Glyceraldehyde-3-phosphate dehydrogenase; SMA: Smooth muscle actin.

    Article Snippet: Equal amounts of total protein-were separated in 4%-12% bis-tris (BT) gels (NuPAGE, Gibco-BRL Life Technologies, Grand Island, NY), blotted onto Hybond C extra membranes, blocked overnight in 5% milk, and incubated with antibodies against α smooth muscle actin (αSMA), glyceraldehyde-3-phosphate dehydrogenase (GAPDH) (Santa Cruz Biotechnology, Santa Cruz, CA, United States) and then incubated with horseradish peroxidase-conjugated secondary antibody.

    Techniques: Expressing

    Efficient differentiation of mesenchymal stem cells (MSCs) from human induced pluripotent stem cells (iPSCs) and characterization of iMSCs-Exo. (A) Phase-contrast image of long-term cultured iPSC clone before differentiation (i), intermediate phase of induced iPSCs (ii), and iMSCs (iii). (B) Quantitative reverse-transcriptase polymerase chain reaction analysis of the expression level of pluripotent genes (Nanog, Oct4, and Msx1) in iPSCs during differentiation (i) and in iPSCs and iMSCs (ii). (C) Flow cytometric analysis of mesenchymal positive markers, such as CD29, CD44, CD73, CD90, CD105, and CD146, and negative markers, such as CD34, CD45, CD133, and HLA-DR. Black histograms represent the isotype controls, and the red solid peak represents the marker indicated. (D) Multi-differentiation potential of iMSCs. (i) Alizarin Red staining of osteogenic mineralization (day 14). (ii) Oil Red O staining of small lipid droplets (day 21). (iii) Toluidine Blue staining of cartilaginous extracellular matrix (day 21). (E) Morphology of iMSCs-Exo under transmission electron microscopy. (F) Western blotting analysis of exosomal CD63, CD81, and CD9 protein in iMSCs and iMSCs-Exo. The culture medium served as the control. GAPDH, glyceraldehyde 3-phosphate dehydrogenase; iMSC, induced pluripotent stem cell-derived mesenchymal stem cell; iMSCs-Exo, exosomes derived from induced pluripotent stem cell-derived mesenchymal stem cells.

    Journal: Stem Cell Research & Therapy

    Article Title: Exosomes secreted by human-induced pluripotent stem cell-derived mesenchymal stem cells attenuate limb ischemia by promoting angiogenesis in mice

    doi: 10.1186/scrt546

    Figure Lengend Snippet: Efficient differentiation of mesenchymal stem cells (MSCs) from human induced pluripotent stem cells (iPSCs) and characterization of iMSCs-Exo. (A) Phase-contrast image of long-term cultured iPSC clone before differentiation (i), intermediate phase of induced iPSCs (ii), and iMSCs (iii). (B) Quantitative reverse-transcriptase polymerase chain reaction analysis of the expression level of pluripotent genes (Nanog, Oct4, and Msx1) in iPSCs during differentiation (i) and in iPSCs and iMSCs (ii). (C) Flow cytometric analysis of mesenchymal positive markers, such as CD29, CD44, CD73, CD90, CD105, and CD146, and negative markers, such as CD34, CD45, CD133, and HLA-DR. Black histograms represent the isotype controls, and the red solid peak represents the marker indicated. (D) Multi-differentiation potential of iMSCs. (i) Alizarin Red staining of osteogenic mineralization (day 14). (ii) Oil Red O staining of small lipid droplets (day 21). (iii) Toluidine Blue staining of cartilaginous extracellular matrix (day 21). (E) Morphology of iMSCs-Exo under transmission electron microscopy. (F) Western blotting analysis of exosomal CD63, CD81, and CD9 protein in iMSCs and iMSCs-Exo. The culture medium served as the control. GAPDH, glyceraldehyde 3-phosphate dehydrogenase; iMSC, induced pluripotent stem cell-derived mesenchymal stem cell; iMSCs-Exo, exosomes derived from induced pluripotent stem cell-derived mesenchymal stem cells.

    Article Snippet: The following human primers were used: Nanog, Oct4, Msx1, hypoxia-inducible factor-1α (HIF-1α), vascular endothelial growth factor-A (VEGFA), VEGFB, placental growth factor (PGF), basic fibroblast growth factor (bFGF), transforming growth factor beta 1 (TGFB1), Angiogenin (Angio), VEGF receptor 2 (kinase insert domain receptor, or KDR), bFGF receptor (bFGFR), and glyceraldehyde 3-phosphate dehydrogenase (GAPDH) (all from Sangon Biotech, Shanghai, China).

    Techniques: Cell Culture, Polymerase Chain Reaction, Expressing, Flow Cytometry, Marker, Staining, Transmission Assay, Electron Microscopy, Western Blot, Derivative Assay

    P2X7 and EGF Receptor Stimulation Regulates the Expression of DUSP6 in Cerebellar Granule Neurons and Astrocytes ) for different times and DUSP6 and Glyceraldehyde-3-phosphate dehydrogenase (GAPDH) were detected in the total lysates by immunoblotting. (A) Immunoblots of a representative time-course experiment for each effector. (B) Cells were incubated with or without the effectors, 300 μM BzATP or 100 ng/mL EGF, for 30 min, fixed and the presence of DUSP6 was detected by immunocytochemistry. Representative immunofluorescence images are shown. Scale bars represent 50 μm. (C) The diagrams represent the quantification of the time courses obtained by normalization to the corresponding GAPDH levels. The data are represented as the means ± SEM from three independent experiments performed on different cultures. *** p

    Journal: Frontiers in Molecular Neuroscience

    Article Title: P2X7 Nucleotide and EGF Receptors Exert Dual Modulation of the Dual-Specificity Phosphatase 6 (MKP-3) in Granule Neurons and Astrocytes, Contributing to Negative Feedback on ERK Signaling

    doi: 10.3389/fnmol.2017.00448

    Figure Lengend Snippet: P2X7 and EGF Receptor Stimulation Regulates the Expression of DUSP6 in Cerebellar Granule Neurons and Astrocytes ) for different times and DUSP6 and Glyceraldehyde-3-phosphate dehydrogenase (GAPDH) were detected in the total lysates by immunoblotting. (A) Immunoblots of a representative time-course experiment for each effector. (B) Cells were incubated with or without the effectors, 300 μM BzATP or 100 ng/mL EGF, for 30 min, fixed and the presence of DUSP6 was detected by immunocytochemistry. Representative immunofluorescence images are shown. Scale bars represent 50 μm. (C) The diagrams represent the quantification of the time courses obtained by normalization to the corresponding GAPDH levels. The data are represented as the means ± SEM from three independent experiments performed on different cultures. *** p

    Article Snippet: Antisera for Glyceraldehyde-3-phosphate dehydrogenase (GAPDH) was generated in rabbit and purchased from Cell Signaling Technology (Beverly, MA, USA).

    Techniques: Expressing, Western Blot, Incubation, Immunocytochemistry, Immunofluorescence

    Effects of increases in dietary LA and dietary OXLAMs on 4-HNE in mouse cerebral cortex. N = 8 tissue samples per group. Immunoblot analysis of cerebral cortex protein lysates for the detection of 4-HNE-modified proteins. Glyceraldehyde 3-phos-phate dehydrogenase (GAPDH) was used as a loading control. Densitometric analysis was performed on background-subtracted blots and normalized to GAPDH. The Low LA group was used as a reference control and set to 1.

    Journal: Biochimica et biophysica acta. Molecular and cell biology of lipids

    Article Title: Effects of diets enriched in linoleic acid and its peroxidation products on brain fatty acids, oxylipins, and aldehydes in mice

    doi: 10.1016/j.bbalip.2018.07.007

    Figure Lengend Snippet: Effects of increases in dietary LA and dietary OXLAMs on 4-HNE in mouse cerebral cortex. N = 8 tissue samples per group. Immunoblot analysis of cerebral cortex protein lysates for the detection of 4-HNE-modified proteins. Glyceraldehyde 3-phos-phate dehydrogenase (GAPDH) was used as a loading control. Densitometric analysis was performed on background-subtracted blots and normalized to GAPDH. The Low LA group was used as a reference control and set to 1.

    Article Snippet: For im-munoblot analysis 40 μg of protein lysate was resolved on Any kD™ Mini-PROTEAN® TGX™ Precast polyacrylamide gels (Biorad, Hercules, CA, USA), transferred to nitrocellulose membrane, blocked in 5% Blottinggrade Blocker (Biorad, Hercules, CA, USA), and incubated with anti-4-HNE antibody (1:1000, Abcam, Cambridge, UK) and Glyceraldehyde 3-phosphate dehydrogenase (GAPDH) (1:10000, Genetex, Irvine, CA, USA) for normalization, followed by incubation with peroxidase-conjugated secondary antibody (Cell Signaling, Danvers, MA, USA).

    Techniques: Modification

    Effect of Phosphoglycerate Kinase 1 (PGK1) on the proliferation of Hepatocellular Carcinoma (HCC) cells. ( A ) Endogenous expression of PGK1 in nomal and five HCC cell lines by Western blotting. Glyceraldehyde 3-phosphate dehydrogenase (GAPDH) was used as internal control. ( B ) Transfection efficiency of PGK1 overexpress and knockdown in HCC. GAPDH was used as internal control. ( C ) Effect of PGK1 on proliferation of SNU182 and JHH5 cells by cell counting kit-8 (CCK8) assay. ( D ) Effect of PGK1 knockdown on proliferation of SNU449 and HCCLM3 cells by CCK8 assay. ( E ) Effect of PGK1 on proliferation of SNU182 and JHH5 cells by plate colony formation assay. ( F ) Effect of PGK1 knockdown on proliferation of SNU449 and HCCLM3 cells by plate colony formation assay. ** p

    Journal: International Journal of Molecular Sciences

    Article Title: PGK1 Drives Hepatocellular Carcinoma Metastasis by Enhancing Metabolic Process

    doi: 10.3390/ijms18081630

    Figure Lengend Snippet: Effect of Phosphoglycerate Kinase 1 (PGK1) on the proliferation of Hepatocellular Carcinoma (HCC) cells. ( A ) Endogenous expression of PGK1 in nomal and five HCC cell lines by Western blotting. Glyceraldehyde 3-phosphate dehydrogenase (GAPDH) was used as internal control. ( B ) Transfection efficiency of PGK1 overexpress and knockdown in HCC. GAPDH was used as internal control. ( C ) Effect of PGK1 on proliferation of SNU182 and JHH5 cells by cell counting kit-8 (CCK8) assay. ( D ) Effect of PGK1 knockdown on proliferation of SNU449 and HCCLM3 cells by CCK8 assay. ( E ) Effect of PGK1 on proliferation of SNU182 and JHH5 cells by plate colony formation assay. ( F ) Effect of PGK1 knockdown on proliferation of SNU449 and HCCLM3 cells by plate colony formation assay. ** p

    Article Snippet: Third, the membrane was blocked with 5% fat-free milk, then incubated with PGK1 (ABclonal Biotech Co., Woburn, MA, USA, 1:100), MYC (ABclonal Biotech Co., Woburn, MA, USA, 1:100), LDHA (ABclonal Biotech Co., Woburn, MA, USA, 1:100), HK2 (ABclonal Biotech Co., Woburn, MA, USA, 1:100), GLUT4 (ABclonal Biotech Co., Woburn, MA, USA, 1:100), or glyceraldehyde 3-phosphate dehydrogenase (GAPDH) (ABclonal Biotech Co., Woburn, MA, USA, 1:1000) at 4 °C overnight.

    Techniques: Expressing, Western Blot, Transfection, Cell Counting, CCK-8 Assay, Colony Assay

    Expression and induction of IL-17R and IL-17RB in IL-17-stimulated FLS from six RA patients. (a) IL-17 dose-dependent changes in the levels of IL-17R and IL-17RB mRNAs. Three independent RA FLS cell lines were stimulated with various amounts of recombinant IL-17 (0 to 20 ng/ml), and subsequent changes in the mRNA levels of IL-17R and IL-17RB were assessed by RT-PCR at 6 hours after the onset of in vitro culture. The relative intensity of each PCR band was normalized against that of GAPDH. Values are the fold increase from the unstimulated cell in each FLS line. (b) Time-dependent changes in the level of IL-17R and IL-17RB mRNAs. Three independent RA FLS cell lines were stimulated with recombinant IL-17, and subsequent changes in the mRNA level of IL-17R and IL-17RB were assessed by RT-PCR 0, 1, 3, 6, 9, and 24 hours after the start of in vitro culture. The relative intensity of each PCR band was normalized against that of GAPDH. Values are the fold increase from the 0 hour measurement in each FLS line. FLS, fibroblast-like synoviocytes; GAPDH, glyceraldehyde-3-phosphate dehydrogenase; IL-17R, IL-17 receptor; IL-17RB, IL-17 receptor B; RA, rheumatoid arthritis.

    Journal: Arthritis Research & Therapy

    Article Title: IL-17 induces production of IL-6 and IL-8 in rheumatoid arthritis synovial fibroblasts via NF-?B- and PI3-kinase/Akt-dependent pathways

    doi: 10.1186/ar1038

    Figure Lengend Snippet: Expression and induction of IL-17R and IL-17RB in IL-17-stimulated FLS from six RA patients. (a) IL-17 dose-dependent changes in the levels of IL-17R and IL-17RB mRNAs. Three independent RA FLS cell lines were stimulated with various amounts of recombinant IL-17 (0 to 20 ng/ml), and subsequent changes in the mRNA levels of IL-17R and IL-17RB were assessed by RT-PCR at 6 hours after the onset of in vitro culture. The relative intensity of each PCR band was normalized against that of GAPDH. Values are the fold increase from the unstimulated cell in each FLS line. (b) Time-dependent changes in the level of IL-17R and IL-17RB mRNAs. Three independent RA FLS cell lines were stimulated with recombinant IL-17, and subsequent changes in the mRNA level of IL-17R and IL-17RB were assessed by RT-PCR 0, 1, 3, 6, 9, and 24 hours after the start of in vitro culture. The relative intensity of each PCR band was normalized against that of GAPDH. Values are the fold increase from the 0 hour measurement in each FLS line. FLS, fibroblast-like synoviocytes; GAPDH, glyceraldehyde-3-phosphate dehydrogenase; IL-17R, IL-17 receptor; IL-17RB, IL-17 receptor B; RA, rheumatoid arthritis.

    Article Snippet: PCR amplification of IL-17 receptors, as well as glyceraldehyde-3-phosphate dehydrogenase (GAPDH) as a quantitation control, were done by rTaq polymerase (Takara Shuzo, Shiga, Japan) and the following primers: IL-17R, sense 5'-GGGATTACAGGCGTGAGCCA-3', antisense 5'-GCGGTCTGGTTATCGTCTAT-3'; IL-17RB, sense 5'-TCATCTGCACAACTCCGTGG-3', antisense 5'-TCGAATGTTAAGGCTACATT-3'; and GAPDH, sense 5'-CGATGCTGGGCGTGAGTAC-3', antisense 5'-CGT-TCAGCTCAGGGATGACC-3'.

    Techniques: Expressing, Recombinant, Reverse Transcription Polymerase Chain Reaction, In Vitro, Polymerase Chain Reaction

    GROβ mRNA levels in LSCC tissues were significantly increased compared with corresponding non-cancerous tissues, as determined by reverse transcription-quantitative polymerase chain reaction, with data normalized to GAPDH mRNA levels (P=0.009). GROβ, growth-related gene product β; LSCC, laryngeal squamous cell carcinoma; GADPH, glyceraldehyde 3-phosphate dehydrogenase.

    Journal: Oncology Letters

    Article Title: The prognostic implications of growth-related gene product β in laryngeal squamous cell carcinoma

    doi: 10.3892/ol.2017.6604

    Figure Lengend Snippet: GROβ mRNA levels in LSCC tissues were significantly increased compared with corresponding non-cancerous tissues, as determined by reverse transcription-quantitative polymerase chain reaction, with data normalized to GAPDH mRNA levels (P=0.009). GROβ, growth-related gene product β; LSCC, laryngeal squamous cell carcinoma; GADPH, glyceraldehyde 3-phosphate dehydrogenase.

    Article Snippet: Expression levels of GROβ and glyceraldehyde 3-phosphate dehydrogenase (GAPDH) were determined by RT-qPCR using the iQ5 detection system (Bio-Rad Laboratories, Inc., Hercules, CA, USA) and the SensiMix One-Step SYBR-Green kit (Quantace Ltd., London, UK).

    Techniques: Real-time Polymerase Chain Reaction

    The effect of esculetin on the expression of cell cycle arrest- and apoptosis-related proteins in human malignant melanoma. (A) The G361 cells were incubated with esculetin (20, 40, and 80 μg/mL) or without for 48 hours. The cell lysates were determined using Western blot analysis with antibodies against p27, p21, and cyclin D1. Equal loading protein was confirmed using glyceraldehyde-3-phosphate de-hydrogenase (GAPDH). (B) The G361 cells were treated with esculetin (0, 20, 40, and 80 μg/mL) for 48 hours. The cell lysates were then determined via Western blot analysis with antibodies against procaspase 3, active caspase 3, Bax, and PARP. Equal loading protein was confirmed using GAPDH. The values were measured using Image J densitometry, and the data were expressed as a representative of two or three independent experiments.

    Journal: Journal of Cancer Prevention

    Article Title: Esculetin, a Coumarin Derivative, Exhibits Anti-proliferative and Pro-apoptotic Activity in G361 Human Malignant Melanoma

    doi: 10.15430/JCP.2015.20.2.106

    Figure Lengend Snippet: The effect of esculetin on the expression of cell cycle arrest- and apoptosis-related proteins in human malignant melanoma. (A) The G361 cells were incubated with esculetin (20, 40, and 80 μg/mL) or without for 48 hours. The cell lysates were determined using Western blot analysis with antibodies against p27, p21, and cyclin D1. Equal loading protein was confirmed using glyceraldehyde-3-phosphate de-hydrogenase (GAPDH). (B) The G361 cells were treated with esculetin (0, 20, 40, and 80 μg/mL) for 48 hours. The cell lysates were then determined via Western blot analysis with antibodies against procaspase 3, active caspase 3, Bax, and PARP. Equal loading protein was confirmed using GAPDH. The values were measured using Image J densitometry, and the data were expressed as a representative of two or three independent experiments.

    Article Snippet: The primary antibodies used in this study were as followings: α-Sp1, α-p27, α-p21, α-cyclinD1 (Santa Cruz Biotechnology Inc., Santa Cruz, CA, USA), α-procaspase 3, α-cleaved caspase 3 (Cell Signaling Technology, Denvers, MA, USA), and glyceraldehyde-3-phosphate dehydrogenase (GAPDH) (AbFrontier, Seoul, Korea).

    Techniques: Expressing, Incubation, Western Blot

    The effect of esculetin on Sp1 expression in human malignant melanoma. (A) The G361 cells were treated with different concentrations of esculetin (0, 20, 40, and 80 μg/mL) for 48 hours. The cell lysates were separated by SDS PAGE, and then the membranes were transferred from SDS PAGE gels subjected to Western blot analysis for Sp1. An equal loading protein was confirmed using glyceraldehyde-3-phosphate dehydrogenase (GAPDH). The histogram showed the ratio of Sp1 to GAPDH expression, and the results were expressed as the average of a triplicate sample from three independent experiments. The asterisk suggested P

    Journal: Journal of Cancer Prevention

    Article Title: Esculetin, a Coumarin Derivative, Exhibits Anti-proliferative and Pro-apoptotic Activity in G361 Human Malignant Melanoma

    doi: 10.15430/JCP.2015.20.2.106

    Figure Lengend Snippet: The effect of esculetin on Sp1 expression in human malignant melanoma. (A) The G361 cells were treated with different concentrations of esculetin (0, 20, 40, and 80 μg/mL) for 48 hours. The cell lysates were separated by SDS PAGE, and then the membranes were transferred from SDS PAGE gels subjected to Western blot analysis for Sp1. An equal loading protein was confirmed using glyceraldehyde-3-phosphate dehydrogenase (GAPDH). The histogram showed the ratio of Sp1 to GAPDH expression, and the results were expressed as the average of a triplicate sample from three independent experiments. The asterisk suggested P

    Article Snippet: The primary antibodies used in this study were as followings: α-Sp1, α-p27, α-p21, α-cyclinD1 (Santa Cruz Biotechnology Inc., Santa Cruz, CA, USA), α-procaspase 3, α-cleaved caspase 3 (Cell Signaling Technology, Denvers, MA, USA), and glyceraldehyde-3-phosphate dehydrogenase (GAPDH) (AbFrontier, Seoul, Korea).

    Techniques: Expressing, SDS Page, Western Blot