Journal: International Journal of Molecular Medicine
Article Title: Aspirin promotes apoptosis and inhibits proliferation by blocking G0/G1 into S phase in rheumatoid arthritis fibroblast-like synoviocytes via downregulation of JAK/STAT3 and NF-κB signaling pathway
Figure Lengend Snippet: Effects of aspirin on Bcl-2, BAX, PARP1, Cyclin D1 and P21 expression in RA-FLS. (A) After RA-FLS were exposed to various concentration of aspirin for 24 h, western blotting was used to determine Bcl-2, BAX, PARP1, Cyclin D1 and P21 protein levels. The corresponding internal control was GADPH. Changes in Bcl-2, BAX, PARP1, Cyclin D1 and P21 protein expression in RA-FLS treated with aspirin are shown: Bcl-2, PARP1, Cyclin D1 and P21 were decreased while BAX was increased, each with increasing aspirin concentration. (B) Bar graph shows the gray value analysis of Bcl-2, BAX, PARP1, Cyclin D1 and P21. Data are presented as the means ± SD (error bars) from three independent experiments. * P
Article Snippet: The membranes were blocked with 5% BSA in TBS-T for 1 h, and then incubated with rabbit anti-Bax (1:2,000 dilution, Proteintech Group; #S0599-2-lg), anti-Bcl-2 (1:1,000 dilution, Santa Cruz Biotechnology, Inc.; #sc-7382), anti-PARP1 (1:2,000 dilution, Proteintech Group; #13371-1-AP), anti-P21 (1:1,000 dilution, #EPR3993), anti-cyclin D1 (1:10,000 dilution, #EPR2241; both from Abcam), anti-P65 (1:2,000 dilution; #8242), anti-p-P65 (1:2,000 dilution; #3033; both from Cell Signaling Technology, Inc.), anti-P50/P105 (1:1,000 dilution, Abcam: #E381), anti-p-P50/105 (1:1,000 dilution, Affinity Company: #AF3219), anti-STAT3 (1:2,000 dilution, #EPR787Y) and anti-p-STAT3 (1:200,000 dilution, #EP2147Y; both from Abcam) antibodies for 2 h at room temperature.
Techniques: Expressing, Concentration Assay, Western Blot