glutathione sepharose 4b beads Ge Healthcare Search Results


  • Logo
  • About
  • News
  • Press Release
  • Team
  • Advisors
  • Partners
  • Contact
  • Bioz Stars
  • Bioz vStars
  • 99
    GE Healthcare glutathione sepharose 4b
    Glutathione Sepharose 4b, supplied by GE Healthcare, used in various techniques. Bioz Stars score: 99/100, based on 18170 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/glutathione sepharose 4b/product/GE Healthcare
    Average 99 stars, based on 18170 article reviews
    Price from $9.99 to $1999.99
    glutathione sepharose 4b - by Bioz Stars, 2020-01
    99/100 stars
      Buy from Supplier

    99
    GE Healthcare glutathione sepharose 4b beads
    TBK1 phosphorylates RKIP at serine 109 BMDM cells were infected with VSV (MOI 0.1) or HSV (MOI 5) for the indicated times. Cell lysates were analyzed by immunoblotting with the indicated antibodies. BMDM cells were transfected with control or TBK1 siRNA. 72 h after transfection, cells were analyzed by immunoblotting with antibodies for phospho‐RKIP or TBK1. Immunoblot analysis of RKIP phosphorylation in control or TBK1 −/− mouse embryonic fibroblasts after VSV (MOI 0.1) infection for the indicated times. Recombinant flag‐tagged TBK1 was immunoprecipitated with anti‐flag M2 beads in flag‐TBK1‐transfected 293T cells. Followed this, in vitro kinase assay was carried out in the reaction mixture containing 2 mM ATP, 100 μM GST‐RKIP, or 100 μM GST‐GFP together with 5 μM flag‐TBK1 at 30°C for 30 min. Assay mixtures were immunoblotted with the indicated antibodies for phospho‐RKIP, flag, GST, or his. Immunoblot analysis of extracts of RAW264.7 cells stably overexpressing flag‐RKIP, flag‐S109A, and flag‐S109D, and infected with VSV (MOI 0.1) for the indicated times, followed by immunoprecipitation with anti‐flag beads. BMDM cells were infected with VSV (MOI 0.1) for the indicated times. The cell lysates were immunoprecipitated with anti‐TBK1 (left) or anti‐RKIP (right). The immunoprecipitates were analyzed by immunoblot with anti‐RKIP or anti‐TBK1 antibody. The levels of the endogenous RKIP, TBK1, and p‐RKIP were detected by immunoblot analysis. Co‐immunoprecipitation and immunoblot analysis of 293T cells transfected with myc‐TBK1 alone or with flag‐RKIP, flag‐S109A, or flag‐S109D. GST pull‐down experiment was performed with 1 μg fusion protein GST‐GFP, GST‐his‐RKIP, or GST‐his‐S109D mixed with 20 μl pre‐cleared <t>agarose</t> beads in 800 μl reaction medium, followed by adding 1 μg flag‐TBK1 and incubating at 4°C for 3 h with gentle rotation. Pull‐down (lane 1–3) and input samples (lane 4) were separated by SDS–PAGE followed by immunoblotting with anti‐GST or anti‐flag antibody. Immunoprecipitation and immunoblot analysis of 293T cells transfected with indicated combinations of vector for myc‐TBK1, flag‐RKIP, flag‐S109A, flag‐S109D, HA‐GSK3β or flag‐GFP. In vitro assay was carried out in the reaction mixture containing 2 mM ATP, 50 μM GST‐TBK1 together with 100 μM GST‐his‐RKIP, 100 μM GST‐his‐S109D, 100 μM GST‐his‐S109A, or 100 μM GST, respectively, at 30°C for 30 min, followed by immunoblotting with anti‐p‐TBK1 or anti‐GST antibody. . Source data are available online for this figure.
    Glutathione Sepharose 4b Beads, supplied by GE Healthcare, used in various techniques. Bioz Stars score: 99/100, based on 16029 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/glutathione sepharose 4b beads/product/GE Healthcare
    Average 99 stars, based on 16029 article reviews
    Price from $9.99 to $1999.99
    glutathione sepharose 4b beads - by Bioz Stars, 2020-01
    99/100 stars
      Buy from Supplier

    95
    Millipore glutathione sepharose 4b beads
    TBK1 phosphorylates RKIP at serine 109 BMDM cells were infected with VSV (MOI 0.1) or HSV (MOI 5) for the indicated times. Cell lysates were analyzed by immunoblotting with the indicated antibodies. BMDM cells were transfected with control or TBK1 siRNA. 72 h after transfection, cells were analyzed by immunoblotting with antibodies for phospho‐RKIP or TBK1. Immunoblot analysis of RKIP phosphorylation in control or TBK1 −/− mouse embryonic fibroblasts after VSV (MOI 0.1) infection for the indicated times. Recombinant flag‐tagged TBK1 was immunoprecipitated with anti‐flag M2 beads in flag‐TBK1‐transfected 293T cells. Followed this, in vitro kinase assay was carried out in the reaction mixture containing 2 mM ATP, 100 μM GST‐RKIP, or 100 μM GST‐GFP together with 5 μM flag‐TBK1 at 30°C for 30 min. Assay mixtures were immunoblotted with the indicated antibodies for phospho‐RKIP, flag, GST, or his. Immunoblot analysis of extracts of RAW264.7 cells stably overexpressing flag‐RKIP, flag‐S109A, and flag‐S109D, and infected with VSV (MOI 0.1) for the indicated times, followed by immunoprecipitation with anti‐flag beads. BMDM cells were infected with VSV (MOI 0.1) for the indicated times. The cell lysates were immunoprecipitated with anti‐TBK1 (left) or anti‐RKIP (right). The immunoprecipitates were analyzed by immunoblot with anti‐RKIP or anti‐TBK1 antibody. The levels of the endogenous RKIP, TBK1, and p‐RKIP were detected by immunoblot analysis. Co‐immunoprecipitation and immunoblot analysis of 293T cells transfected with myc‐TBK1 alone or with flag‐RKIP, flag‐S109A, or flag‐S109D. GST pull‐down experiment was performed with 1 μg fusion protein GST‐GFP, GST‐his‐RKIP, or GST‐his‐S109D mixed with 20 μl pre‐cleared <t>agarose</t> beads in 800 μl reaction medium, followed by adding 1 μg flag‐TBK1 and incubating at 4°C for 3 h with gentle rotation. Pull‐down (lane 1–3) and input samples (lane 4) were separated by SDS–PAGE followed by immunoblotting with anti‐GST or anti‐flag antibody. Immunoprecipitation and immunoblot analysis of 293T cells transfected with indicated combinations of vector for myc‐TBK1, flag‐RKIP, flag‐S109A, flag‐S109D, HA‐GSK3β or flag‐GFP. In vitro assay was carried out in the reaction mixture containing 2 mM ATP, 50 μM GST‐TBK1 together with 100 μM GST‐his‐RKIP, 100 μM GST‐his‐S109D, 100 μM GST‐his‐S109A, or 100 μM GST, respectively, at 30°C for 30 min, followed by immunoblotting with anti‐p‐TBK1 or anti‐GST antibody. . Source data are available online for this figure.
    Glutathione Sepharose 4b Beads, supplied by Millipore, used in various techniques. Bioz Stars score: 95/100, based on 241 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/glutathione sepharose 4b beads/product/Millipore
    Average 95 stars, based on 241 article reviews
    Price from $9.99 to $1999.99
    glutathione sepharose 4b beads - by Bioz Stars, 2020-01
    95/100 stars
      Buy from Supplier

    93
    GE Healthcare glutathione sepharose 4b slurry beads
    TBK1 phosphorylates RKIP at serine 109 BMDM cells were infected with VSV (MOI 0.1) or HSV (MOI 5) for the indicated times. Cell lysates were analyzed by immunoblotting with the indicated antibodies. BMDM cells were transfected with control or TBK1 siRNA. 72 h after transfection, cells were analyzed by immunoblotting with antibodies for phospho‐RKIP or TBK1. Immunoblot analysis of RKIP phosphorylation in control or TBK1 −/− mouse embryonic fibroblasts after VSV (MOI 0.1) infection for the indicated times. Recombinant flag‐tagged TBK1 was immunoprecipitated with anti‐flag M2 beads in flag‐TBK1‐transfected 293T cells. Followed this, in vitro kinase assay was carried out in the reaction mixture containing 2 mM ATP, 100 μM GST‐RKIP, or 100 μM GST‐GFP together with 5 μM flag‐TBK1 at 30°C for 30 min. Assay mixtures were immunoblotted with the indicated antibodies for phospho‐RKIP, flag, GST, or his. Immunoblot analysis of extracts of RAW264.7 cells stably overexpressing flag‐RKIP, flag‐S109A, and flag‐S109D, and infected with VSV (MOI 0.1) for the indicated times, followed by immunoprecipitation with anti‐flag beads. BMDM cells were infected with VSV (MOI 0.1) for the indicated times. The cell lysates were immunoprecipitated with anti‐TBK1 (left) or anti‐RKIP (right). The immunoprecipitates were analyzed by immunoblot with anti‐RKIP or anti‐TBK1 antibody. The levels of the endogenous RKIP, TBK1, and p‐RKIP were detected by immunoblot analysis. Co‐immunoprecipitation and immunoblot analysis of 293T cells transfected with myc‐TBK1 alone or with flag‐RKIP, flag‐S109A, or flag‐S109D. GST pull‐down experiment was performed with 1 μg fusion protein GST‐GFP, GST‐his‐RKIP, or GST‐his‐S109D mixed with 20 μl pre‐cleared <t>agarose</t> beads in 800 μl reaction medium, followed by adding 1 μg flag‐TBK1 and incubating at 4°C for 3 h with gentle rotation. Pull‐down (lane 1–3) and input samples (lane 4) were separated by SDS–PAGE followed by immunoblotting with anti‐GST or anti‐flag antibody. Immunoprecipitation and immunoblot analysis of 293T cells transfected with indicated combinations of vector for myc‐TBK1, flag‐RKIP, flag‐S109A, flag‐S109D, HA‐GSK3β or flag‐GFP. In vitro assay was carried out in the reaction mixture containing 2 mM ATP, 50 μM GST‐TBK1 together with 100 μM GST‐his‐RKIP, 100 μM GST‐his‐S109D, 100 μM GST‐his‐S109A, or 100 μM GST, respectively, at 30°C for 30 min, followed by immunoblotting with anti‐p‐TBK1 or anti‐GST antibody. . Source data are available online for this figure.
    Glutathione Sepharose 4b Slurry Beads, supplied by GE Healthcare, used in various techniques. Bioz Stars score: 93/100, based on 78 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/glutathione sepharose 4b slurry beads/product/GE Healthcare
    Average 93 stars, based on 78 article reviews
    Price from $9.99 to $1999.99
    glutathione sepharose 4b slurry beads - by Bioz Stars, 2020-01
    93/100 stars
      Buy from Supplier

    94
    GE Healthcare glutathione sepharose 4b gs4b beads
    TBK1 phosphorylates RKIP at serine 109 BMDM cells were infected with VSV (MOI 0.1) or HSV (MOI 5) for the indicated times. Cell lysates were analyzed by immunoblotting with the indicated antibodies. BMDM cells were transfected with control or TBK1 siRNA. 72 h after transfection, cells were analyzed by immunoblotting with antibodies for phospho‐RKIP or TBK1. Immunoblot analysis of RKIP phosphorylation in control or TBK1 −/− mouse embryonic fibroblasts after VSV (MOI 0.1) infection for the indicated times. Recombinant flag‐tagged TBK1 was immunoprecipitated with anti‐flag M2 beads in flag‐TBK1‐transfected 293T cells. Followed this, in vitro kinase assay was carried out in the reaction mixture containing 2 mM ATP, 100 μM GST‐RKIP, or 100 μM GST‐GFP together with 5 μM flag‐TBK1 at 30°C for 30 min. Assay mixtures were immunoblotted with the indicated antibodies for phospho‐RKIP, flag, GST, or his. Immunoblot analysis of extracts of RAW264.7 cells stably overexpressing flag‐RKIP, flag‐S109A, and flag‐S109D, and infected with VSV (MOI 0.1) for the indicated times, followed by immunoprecipitation with anti‐flag beads. BMDM cells were infected with VSV (MOI 0.1) for the indicated times. The cell lysates were immunoprecipitated with anti‐TBK1 (left) or anti‐RKIP (right). The immunoprecipitates were analyzed by immunoblot with anti‐RKIP or anti‐TBK1 antibody. The levels of the endogenous RKIP, TBK1, and p‐RKIP were detected by immunoblot analysis. Co‐immunoprecipitation and immunoblot analysis of 293T cells transfected with myc‐TBK1 alone or with flag‐RKIP, flag‐S109A, or flag‐S109D. GST pull‐down experiment was performed with 1 μg fusion protein GST‐GFP, GST‐his‐RKIP, or GST‐his‐S109D mixed with 20 μl pre‐cleared <t>agarose</t> beads in 800 μl reaction medium, followed by adding 1 μg flag‐TBK1 and incubating at 4°C for 3 h with gentle rotation. Pull‐down (lane 1–3) and input samples (lane 4) were separated by SDS–PAGE followed by immunoblotting with anti‐GST or anti‐flag antibody. Immunoprecipitation and immunoblot analysis of 293T cells transfected with indicated combinations of vector for myc‐TBK1, flag‐RKIP, flag‐S109A, flag‐S109D, HA‐GSK3β or flag‐GFP. In vitro assay was carried out in the reaction mixture containing 2 mM ATP, 50 μM GST‐TBK1 together with 100 μM GST‐his‐RKIP, 100 μM GST‐his‐S109D, 100 μM GST‐his‐S109A, or 100 μM GST, respectively, at 30°C for 30 min, followed by immunoblotting with anti‐p‐TBK1 or anti‐GST antibody. . Source data are available online for this figure.
    Glutathione Sepharose 4b Gs4b Beads, supplied by GE Healthcare, used in various techniques. Bioz Stars score: 94/100, based on 65 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/glutathione sepharose 4b gs4b beads/product/GE Healthcare
    Average 94 stars, based on 65 article reviews
    Price from $9.99 to $1999.99
    glutathione sepharose 4b gs4b beads - by Bioz Stars, 2020-01
    94/100 stars
      Buy from Supplier

    84
    GE Healthcare glutathione sepharose 4b matrix beads
    TBK1 phosphorylates RKIP at serine 109 BMDM cells were infected with VSV (MOI 0.1) or HSV (MOI 5) for the indicated times. Cell lysates were analyzed by immunoblotting with the indicated antibodies. BMDM cells were transfected with control or TBK1 siRNA. 72 h after transfection, cells were analyzed by immunoblotting with antibodies for phospho‐RKIP or TBK1. Immunoblot analysis of RKIP phosphorylation in control or TBK1 −/− mouse embryonic fibroblasts after VSV (MOI 0.1) infection for the indicated times. Recombinant flag‐tagged TBK1 was immunoprecipitated with anti‐flag M2 beads in flag‐TBK1‐transfected 293T cells. Followed this, in vitro kinase assay was carried out in the reaction mixture containing 2 mM ATP, 100 μM GST‐RKIP, or 100 μM GST‐GFP together with 5 μM flag‐TBK1 at 30°C for 30 min. Assay mixtures were immunoblotted with the indicated antibodies for phospho‐RKIP, flag, GST, or his. Immunoblot analysis of extracts of RAW264.7 cells stably overexpressing flag‐RKIP, flag‐S109A, and flag‐S109D, and infected with VSV (MOI 0.1) for the indicated times, followed by immunoprecipitation with anti‐flag beads. BMDM cells were infected with VSV (MOI 0.1) for the indicated times. The cell lysates were immunoprecipitated with anti‐TBK1 (left) or anti‐RKIP (right). The immunoprecipitates were analyzed by immunoblot with anti‐RKIP or anti‐TBK1 antibody. The levels of the endogenous RKIP, TBK1, and p‐RKIP were detected by immunoblot analysis. Co‐immunoprecipitation and immunoblot analysis of 293T cells transfected with myc‐TBK1 alone or with flag‐RKIP, flag‐S109A, or flag‐S109D. GST pull‐down experiment was performed with 1 μg fusion protein GST‐GFP, GST‐his‐RKIP, or GST‐his‐S109D mixed with 20 μl pre‐cleared <t>agarose</t> beads in 800 μl reaction medium, followed by adding 1 μg flag‐TBK1 and incubating at 4°C for 3 h with gentle rotation. Pull‐down (lane 1–3) and input samples (lane 4) were separated by SDS–PAGE followed by immunoblotting with anti‐GST or anti‐flag antibody. Immunoprecipitation and immunoblot analysis of 293T cells transfected with indicated combinations of vector for myc‐TBK1, flag‐RKIP, flag‐S109A, flag‐S109D, HA‐GSK3β or flag‐GFP. In vitro assay was carried out in the reaction mixture containing 2 mM ATP, 50 μM GST‐TBK1 together with 100 μM GST‐his‐RKIP, 100 μM GST‐his‐S109D, 100 μM GST‐his‐S109A, or 100 μM GST, respectively, at 30°C for 30 min, followed by immunoblotting with anti‐p‐TBK1 or anti‐GST antibody. . Source data are available online for this figure.
    Glutathione Sepharose 4b Matrix Beads, supplied by GE Healthcare, used in various techniques. Bioz Stars score: 84/100, based on 21 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/glutathione sepharose 4b matrix beads/product/GE Healthcare
    Average 84 stars, based on 21 article reviews
    Price from $9.99 to $1999.99
    glutathione sepharose 4b matrix beads - by Bioz Stars, 2020-01
    84/100 stars
      Buy from Supplier

    93
    GE Healthcare glutathione gsh sepharose 4b beads
    Interaction of Siah2 with PHD3. ( a ) HEK293 cells were transfected in 60-mm cell culture plates for 2 days with the indicated expression plasmids. The cells were lysed, and the lysates were subjected to FLAG immunoprecipitation (IP), as described under “ Materials and Methods ”. Aliquots of the cell lysates and immunoprecipitates were analyzed by western blotting with the anti-HA antibody. Both full length Siah2 and Siah2 SBD bind to PHD3 to the same extent. In the IP, the presence of the faint band in the empty vector lane is due to non-specific binding of PHD3. The same membrane was reblotted with FLAG antibody to detect FLAG tagged Siah2 proteins. ( b ) GST-Siah2 SBD pulldown of HA-PHD3. Cell lysate of HEK293 cells transfected with HA-PHD3 was incubated with GST-Siah2 SBD immobilized on <t>GSH</t> <t>agarose</t> beads and the reaction was performed as described under “ Material and Methods ”. The empty expression vector alone was expressed as a GST control for non-specific binding of HA PHD3. After the incubation, the lysate was removed, the GSH-agarose beads were washed, and bound HA-PHD3 was analyzed by Western blotting using anti HA antibody. The pull down assay confirmed the interaction of Siah2 SBD with PHD3.
    Glutathione Gsh Sepharose 4b Beads, supplied by GE Healthcare, used in various techniques. Bioz Stars score: 93/100, based on 71 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/glutathione gsh sepharose 4b beads/product/GE Healthcare
    Average 93 stars, based on 71 article reviews
    Price from $9.99 to $1999.99
    glutathione gsh sepharose 4b beads - by Bioz Stars, 2020-01
    93/100 stars
      Buy from Supplier

    70
    GE Healthcare packed glutathione sepharose 4b beads
    Interaction of Siah2 with PHD3. ( a ) HEK293 cells were transfected in 60-mm cell culture plates for 2 days with the indicated expression plasmids. The cells were lysed, and the lysates were subjected to FLAG immunoprecipitation (IP), as described under “ Materials and Methods ”. Aliquots of the cell lysates and immunoprecipitates were analyzed by western blotting with the anti-HA antibody. Both full length Siah2 and Siah2 SBD bind to PHD3 to the same extent. In the IP, the presence of the faint band in the empty vector lane is due to non-specific binding of PHD3. The same membrane was reblotted with FLAG antibody to detect FLAG tagged Siah2 proteins. ( b ) GST-Siah2 SBD pulldown of HA-PHD3. Cell lysate of HEK293 cells transfected with HA-PHD3 was incubated with GST-Siah2 SBD immobilized on <t>GSH</t> <t>agarose</t> beads and the reaction was performed as described under “ Material and Methods ”. The empty expression vector alone was expressed as a GST control for non-specific binding of HA PHD3. After the incubation, the lysate was removed, the GSH-agarose beads were washed, and bound HA-PHD3 was analyzed by Western blotting using anti HA antibody. The pull down assay confirmed the interaction of Siah2 SBD with PHD3.
    Packed Glutathione Sepharose 4b Beads, supplied by GE Healthcare, used in various techniques. Bioz Stars score: 70/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/packed glutathione sepharose 4b beads/product/GE Healthcare
    Average 70 stars, based on 1 article reviews
    Price from $9.99 to $1999.99
    packed glutathione sepharose 4b beads - by Bioz Stars, 2020-01
    70/100 stars
      Buy from Supplier

    79
    GE Healthcare glutathione gst sepharose 4b beads
    The 2BC non-structural protein of EV-A71 interacts with syntaxin-17 (STX17) and synaptosome-associated protein of 29 kDa (SNAP29). ( A ) Yeast two-hybrid assays of 2BC and STX17. Yeasts were transformed with pGBKT7 or pGBKT7-STX17 together with pACT2 or pACT2–2BC. Yeasts were then grown on selective medium without tryptophan (-W) or without leucine, tryptophan, and histidine (-LWH); ( B ) HeLa cells were transfected with pDEST27 or pDEST27–2BC in combination with pCherry-STX17 for 48 h. The transfected cells were examined under a confocal microscope with objective 63× (scale bar 10µm) for the presence of co-localization; and ( C ) the Pearson’s correlation coefficient was determined; ( D ) HEK-293T cells were co-transfected with FLAG-STX17 and <t>GST-2BC</t> for 48 h. Lysates were collected for co-immunoprecipitation (IP) using glutathione (GST) <t>sepharose.</t> Western Blot was performed to detect FLAG-STX17 and GST fusion proteins; ( E ) RD cells were infected with EV-A71 for 8 hpi and lysates of infected cells were then harvested prior to co-immunoprecipitation (co-IP) assay using anti-STX17 and anti-SNAP29. The final IP eluates and discarded IP flow through were collected for WB to detect 2BC (anti-2C polyclonal antibody) and structural proteins (mAB979) of EV-A71. One-way ANOVA: * p
    Glutathione Gst Sepharose 4b Beads, supplied by GE Healthcare, used in various techniques. Bioz Stars score: 79/100, based on 6 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/glutathione gst sepharose 4b beads/product/GE Healthcare
    Average 79 stars, based on 6 article reviews
    Price from $9.99 to $1999.99
    glutathione gst sepharose 4b beads - by Bioz Stars, 2020-01
    79/100 stars
      Buy from Supplier

    97
    GE Healthcare pre washed glutathione sepharose 4b beads
    The 2BC non-structural protein of EV-A71 interacts with syntaxin-17 (STX17) and synaptosome-associated protein of 29 kDa (SNAP29). ( A ) Yeast two-hybrid assays of 2BC and STX17. Yeasts were transformed with pGBKT7 or pGBKT7-STX17 together with pACT2 or pACT2–2BC. Yeasts were then grown on selective medium without tryptophan (-W) or without leucine, tryptophan, and histidine (-LWH); ( B ) HeLa cells were transfected with pDEST27 or pDEST27–2BC in combination with pCherry-STX17 for 48 h. The transfected cells were examined under a confocal microscope with objective 63× (scale bar 10µm) for the presence of co-localization; and ( C ) the Pearson’s correlation coefficient was determined; ( D ) HEK-293T cells were co-transfected with FLAG-STX17 and <t>GST-2BC</t> for 48 h. Lysates were collected for co-immunoprecipitation (IP) using glutathione (GST) <t>sepharose.</t> Western Blot was performed to detect FLAG-STX17 and GST fusion proteins; ( E ) RD cells were infected with EV-A71 for 8 hpi and lysates of infected cells were then harvested prior to co-immunoprecipitation (co-IP) assay using anti-STX17 and anti-SNAP29. The final IP eluates and discarded IP flow through were collected for WB to detect 2BC (anti-2C polyclonal antibody) and structural proteins (mAB979) of EV-A71. One-way ANOVA: * p
    Pre Washed Glutathione Sepharose 4b Beads, supplied by GE Healthcare, used in various techniques. Bioz Stars score: 97/100, based on 35 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/pre washed glutathione sepharose 4b beads/product/GE Healthcare
    Average 97 stars, based on 35 article reviews
    Price from $9.99 to $1999.99
    pre washed glutathione sepharose 4b beads - by Bioz Stars, 2020-01
    97/100 stars
      Buy from Supplier

    99
    GE Healthcare glutathione sepharose 4b bead slurry
    The 2BC non-structural protein of EV-A71 interacts with syntaxin-17 (STX17) and synaptosome-associated protein of 29 kDa (SNAP29). ( A ) Yeast two-hybrid assays of 2BC and STX17. Yeasts were transformed with pGBKT7 or pGBKT7-STX17 together with pACT2 or pACT2–2BC. Yeasts were then grown on selective medium without tryptophan (-W) or without leucine, tryptophan, and histidine (-LWH); ( B ) HeLa cells were transfected with pDEST27 or pDEST27–2BC in combination with pCherry-STX17 for 48 h. The transfected cells were examined under a confocal microscope with objective 63× (scale bar 10µm) for the presence of co-localization; and ( C ) the Pearson’s correlation coefficient was determined; ( D ) HEK-293T cells were co-transfected with FLAG-STX17 and <t>GST-2BC</t> for 48 h. Lysates were collected for co-immunoprecipitation (IP) using glutathione (GST) <t>sepharose.</t> Western Blot was performed to detect FLAG-STX17 and GST fusion proteins; ( E ) RD cells were infected with EV-A71 for 8 hpi and lysates of infected cells were then harvested prior to co-immunoprecipitation (co-IP) assay using anti-STX17 and anti-SNAP29. The final IP eluates and discarded IP flow through were collected for WB to detect 2BC (anti-2C polyclonal antibody) and structural proteins (mAB979) of EV-A71. One-way ANOVA: * p
    Glutathione Sepharose 4b Bead Slurry, supplied by GE Healthcare, used in various techniques. Bioz Stars score: 99/100, based on 96 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/glutathione sepharose 4b bead slurry/product/GE Healthcare
    Average 99 stars, based on 96 article reviews
    Price from $9.99 to $1999.99
    glutathione sepharose 4b bead slurry - by Bioz Stars, 2020-01
    99/100 stars
      Buy from Supplier

    70
    GE Healthcare 50 50 glutathione sepharose 4b beads
    The 2BC non-structural protein of EV-A71 interacts with syntaxin-17 (STX17) and synaptosome-associated protein of 29 kDa (SNAP29). ( A ) Yeast two-hybrid assays of 2BC and STX17. Yeasts were transformed with pGBKT7 or pGBKT7-STX17 together with pACT2 or pACT2–2BC. Yeasts were then grown on selective medium without tryptophan (-W) or without leucine, tryptophan, and histidine (-LWH); ( B ) HeLa cells were transfected with pDEST27 or pDEST27–2BC in combination with pCherry-STX17 for 48 h. The transfected cells were examined under a confocal microscope with objective 63× (scale bar 10µm) for the presence of co-localization; and ( C ) the Pearson’s correlation coefficient was determined; ( D ) HEK-293T cells were co-transfected with FLAG-STX17 and <t>GST-2BC</t> for 48 h. Lysates were collected for co-immunoprecipitation (IP) using glutathione (GST) <t>sepharose.</t> Western Blot was performed to detect FLAG-STX17 and GST fusion proteins; ( E ) RD cells were infected with EV-A71 for 8 hpi and lysates of infected cells were then harvested prior to co-immunoprecipitation (co-IP) assay using anti-STX17 and anti-SNAP29. The final IP eluates and discarded IP flow through were collected for WB to detect 2BC (anti-2C polyclonal antibody) and structural proteins (mAB979) of EV-A71. One-way ANOVA: * p
    50 50 Glutathione Sepharose 4b Beads, supplied by GE Healthcare, used in various techniques. Bioz Stars score: 70/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/50 50 glutathione sepharose 4b beads/product/GE Healthcare
    Average 70 stars, based on 1 article reviews
    Price from $9.99 to $1999.99
    50 50 glutathione sepharose 4b beads - by Bioz Stars, 2020-01
    70/100 stars
      Buy from Supplier

    79
    GE Healthcare pulldown assay glutathione sepharose 4b beads
    The 2BC non-structural protein of EV-A71 interacts with syntaxin-17 (STX17) and synaptosome-associated protein of 29 kDa (SNAP29). ( A ) Yeast two-hybrid assays of 2BC and STX17. Yeasts were transformed with pGBKT7 or pGBKT7-STX17 together with pACT2 or pACT2–2BC. Yeasts were then grown on selective medium without tryptophan (-W) or without leucine, tryptophan, and histidine (-LWH); ( B ) HeLa cells were transfected with pDEST27 or pDEST27–2BC in combination with pCherry-STX17 for 48 h. The transfected cells were examined under a confocal microscope with objective 63× (scale bar 10µm) for the presence of co-localization; and ( C ) the Pearson’s correlation coefficient was determined; ( D ) HEK-293T cells were co-transfected with FLAG-STX17 and <t>GST-2BC</t> for 48 h. Lysates were collected for co-immunoprecipitation (IP) using glutathione (GST) <t>sepharose.</t> Western Blot was performed to detect FLAG-STX17 and GST fusion proteins; ( E ) RD cells were infected with EV-A71 for 8 hpi and lysates of infected cells were then harvested prior to co-immunoprecipitation (co-IP) assay using anti-STX17 and anti-SNAP29. The final IP eluates and discarded IP flow through were collected for WB to detect 2BC (anti-2C polyclonal antibody) and structural proteins (mAB979) of EV-A71. One-way ANOVA: * p
    Pulldown Assay Glutathione Sepharose 4b Beads, supplied by GE Healthcare, used in various techniques. Bioz Stars score: 79/100, based on 4 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/pulldown assay glutathione sepharose 4b beads/product/GE Healthcare
    Average 79 stars, based on 4 article reviews
    Price from $9.99 to $1999.99
    pulldown assay glutathione sepharose 4b beads - by Bioz Stars, 2020-01
    79/100 stars
      Buy from Supplier

    83
    GE Healthcare pbsti equilibrated glutathione sepharose 4b beads
    The 2BC non-structural protein of EV-A71 interacts with syntaxin-17 (STX17) and synaptosome-associated protein of 29 kDa (SNAP29). ( A ) Yeast two-hybrid assays of 2BC and STX17. Yeasts were transformed with pGBKT7 or pGBKT7-STX17 together with pACT2 or pACT2–2BC. Yeasts were then grown on selective medium without tryptophan (-W) or without leucine, tryptophan, and histidine (-LWH); ( B ) HeLa cells were transfected with pDEST27 or pDEST27–2BC in combination with pCherry-STX17 for 48 h. The transfected cells were examined under a confocal microscope with objective 63× (scale bar 10µm) for the presence of co-localization; and ( C ) the Pearson’s correlation coefficient was determined; ( D ) HEK-293T cells were co-transfected with FLAG-STX17 and <t>GST-2BC</t> for 48 h. Lysates were collected for co-immunoprecipitation (IP) using glutathione (GST) <t>sepharose.</t> Western Blot was performed to detect FLAG-STX17 and GST fusion proteins; ( E ) RD cells were infected with EV-A71 for 8 hpi and lysates of infected cells were then harvested prior to co-immunoprecipitation (co-IP) assay using anti-STX17 and anti-SNAP29. The final IP eluates and discarded IP flow through were collected for WB to detect 2BC (anti-2C polyclonal antibody) and structural proteins (mAB979) of EV-A71. One-way ANOVA: * p
    Pbsti Equilibrated Glutathione Sepharose 4b Beads, supplied by GE Healthcare, used in various techniques. Bioz Stars score: 83/100, based on 8 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/pbsti equilibrated glutathione sepharose 4b beads/product/GE Healthcare
    Average 83 stars, based on 8 article reviews
    Price from $9.99 to $1999.99
    pbsti equilibrated glutathione sepharose 4b beads - by Bioz Stars, 2020-01
    83/100 stars
      Buy from Supplier

    90
    GE Healthcare glutathione conjugated sepharose 4b beads
    The 2BC non-structural protein of EV-A71 interacts with syntaxin-17 (STX17) and synaptosome-associated protein of 29 kDa (SNAP29). ( A ) Yeast two-hybrid assays of 2BC and STX17. Yeasts were transformed with pGBKT7 or pGBKT7-STX17 together with pACT2 or pACT2–2BC. Yeasts were then grown on selective medium without tryptophan (-W) or without leucine, tryptophan, and histidine (-LWH); ( B ) HeLa cells were transfected with pDEST27 or pDEST27–2BC in combination with pCherry-STX17 for 48 h. The transfected cells were examined under a confocal microscope with objective 63× (scale bar 10µm) for the presence of co-localization; and ( C ) the Pearson’s correlation coefficient was determined; ( D ) HEK-293T cells were co-transfected with FLAG-STX17 and <t>GST-2BC</t> for 48 h. Lysates were collected for co-immunoprecipitation (IP) using glutathione (GST) <t>sepharose.</t> Western Blot was performed to detect FLAG-STX17 and GST fusion proteins; ( E ) RD cells were infected with EV-A71 for 8 hpi and lysates of infected cells were then harvested prior to co-immunoprecipitation (co-IP) assay using anti-STX17 and anti-SNAP29. The final IP eluates and discarded IP flow through were collected for WB to detect 2BC (anti-2C polyclonal antibody) and structural proteins (mAB979) of EV-A71. One-way ANOVA: * p
    Glutathione Conjugated Sepharose 4b Beads, supplied by GE Healthcare, used in various techniques. Bioz Stars score: 90/100, based on 43 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/glutathione conjugated sepharose 4b beads/product/GE Healthcare
    Average 90 stars, based on 43 article reviews
    Price from $9.99 to $1999.99
    glutathione conjugated sepharose 4b beads - by Bioz Stars, 2020-01
    90/100 stars
      Buy from Supplier

    77
    GE Healthcare mixture glutathione sepharose 4b beads
    Pull-down assays of NtWRKY12, TGA1a, TGA2.1, TGA2.2, and NtNPR1 . GST-proteins were incubated with Strep/HIS purified fusion proteins and complexes were pulled down with <t>Streptactin–Sepharose</t> beads (A) or Glutathione–Sepharose <t>4B</t> beads (B,C) . After SDS-PAGE and Western blotting fusion proteins were detected with anti-GST antibodies (A) or anti-HIS antibodies (B,C) . Plus and minus signs denote the presence or absence in the incubation mixtures of the proteins indicated at the left. The input protein was loaded separately on gel and is indicated by (I). The table in (D) summarizes the results of the pull-down assays. Plus-sign, interaction, minus-sign, no interaction, N.D., not determined.
    Mixture Glutathione Sepharose 4b Beads, supplied by GE Healthcare, used in various techniques. Bioz Stars score: 77/100, based on 5 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/mixture glutathione sepharose 4b beads/product/GE Healthcare
    Average 77 stars, based on 5 article reviews
    Price from $9.99 to $1999.99
    mixture glutathione sepharose 4b beads - by Bioz Stars, 2020-01
    77/100 stars
      Buy from Supplier

    94
    GE Healthcare glutathion sepharose 4b beads
    Pull-down assays of NtWRKY12, TGA1a, TGA2.1, TGA2.2, and NtNPR1 . GST-proteins were incubated with Strep/HIS purified fusion proteins and complexes were pulled down with <t>Streptactin–Sepharose</t> beads (A) or Glutathione–Sepharose <t>4B</t> beads (B,C) . After SDS-PAGE and Western blotting fusion proteins were detected with anti-GST antibodies (A) or anti-HIS antibodies (B,C) . Plus and minus signs denote the presence or absence in the incubation mixtures of the proteins indicated at the left. The input protein was loaded separately on gel and is indicated by (I). The table in (D) summarizes the results of the pull-down assays. Plus-sign, interaction, minus-sign, no interaction, N.D., not determined.
    Glutathion Sepharose 4b Beads, supplied by GE Healthcare, used in various techniques. Bioz Stars score: 94/100, based on 49 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/glutathion sepharose 4b beads/product/GE Healthcare
    Average 94 stars, based on 49 article reviews
    Price from $9.99 to $1999.99
    glutathion sepharose 4b beads - by Bioz Stars, 2020-01
    94/100 stars
      Buy from Supplier

    77
    GE Healthcare glutathione sepharose 4b aga rose beads
    Pull-down assays of NtWRKY12, TGA1a, TGA2.1, TGA2.2, and NtNPR1 . GST-proteins were incubated with Strep/HIS purified fusion proteins and complexes were pulled down with <t>Streptactin–Sepharose</t> beads (A) or Glutathione–Sepharose <t>4B</t> beads (B,C) . After SDS-PAGE and Western blotting fusion proteins were detected with anti-GST antibodies (A) or anti-HIS antibodies (B,C) . Plus and minus signs denote the presence or absence in the incubation mixtures of the proteins indicated at the left. The input protein was loaded separately on gel and is indicated by (I). The table in (D) summarizes the results of the pull-down assays. Plus-sign, interaction, minus-sign, no interaction, N.D., not determined.
    Glutathione Sepharose 4b Aga Rose Beads, supplied by GE Healthcare, used in various techniques. Bioz Stars score: 77/100, based on 2 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/glutathione sepharose 4b aga rose beads/product/GE Healthcare
    Average 77 stars, based on 2 article reviews
    Price from $9.99 to $1999.99
    glutathione sepharose 4b aga rose beads - by Bioz Stars, 2020-01
    77/100 stars
      Buy from Supplier

    70
    GE Healthcare glutathione sepharose 4b beads affinity chromatography
    Pull-down assays of NtWRKY12, TGA1a, TGA2.1, TGA2.2, and NtNPR1 . GST-proteins were incubated with Strep/HIS purified fusion proteins and complexes were pulled down with <t>Streptactin–Sepharose</t> beads (A) or Glutathione–Sepharose <t>4B</t> beads (B,C) . After SDS-PAGE and Western blotting fusion proteins were detected with anti-GST antibodies (A) or anti-HIS antibodies (B,C) . Plus and minus signs denote the presence or absence in the incubation mixtures of the proteins indicated at the left. The input protein was loaded separately on gel and is indicated by (I). The table in (D) summarizes the results of the pull-down assays. Plus-sign, interaction, minus-sign, no interaction, N.D., not determined.
    Glutathione Sepharose 4b Beads Affinity Chromatography, supplied by GE Healthcare, used in various techniques. Bioz Stars score: 70/100, based on 2 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/glutathione sepharose 4b beads affinity chromatography/product/GE Healthcare
    Average 70 stars, based on 2 article reviews
    Price from $9.99 to $1999.99
    glutathione sepharose 4b beads affinity chromatography - by Bioz Stars, 2020-01
    70/100 stars
      Buy from Supplier

    91
    GE Healthcare tbs equilibrated glutathione 48 sepharose 4b beads
    Pull-down assays of NtWRKY12, TGA1a, TGA2.1, TGA2.2, and NtNPR1 . GST-proteins were incubated with Strep/HIS purified fusion proteins and complexes were pulled down with <t>Streptactin–Sepharose</t> beads (A) or Glutathione–Sepharose <t>4B</t> beads (B,C) . After SDS-PAGE and Western blotting fusion proteins were detected with anti-GST antibodies (A) or anti-HIS antibodies (B,C) . Plus and minus signs denote the presence or absence in the incubation mixtures of the proteins indicated at the left. The input protein was loaded separately on gel and is indicated by (I). The table in (D) summarizes the results of the pull-down assays. Plus-sign, interaction, minus-sign, no interaction, N.D., not determined.
    Tbs Equilibrated Glutathione 48 Sepharose 4b Beads, supplied by GE Healthcare, used in various techniques. Bioz Stars score: 91/100, based on 18 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/tbs equilibrated glutathione 48 sepharose 4b beads/product/GE Healthcare
    Average 91 stars, based on 18 article reviews
    Price from $9.99 to $1999.99
    tbs equilibrated glutathione 48 sepharose 4b beads - by Bioz Stars, 2020-01
    91/100 stars
      Buy from Supplier

    79
    GE Healthcare gst pull down assay glutathione sepharose 4b beads
    Sds22, PPP1R11, and PP1γ2 are bound to each other in crude testis protein extracts. A . The recombinant proteins <t>GST-PPP1R11</t> and control GST were incubated with testis cell lysates in the presence of <t>Glutathione-Sepharose</t> beads. The eluted proteins and testis extracts alone were resolved by SDS-PAGE and subjected to western blot analysis with anti-Sds22 and anti-PP1γ2 antibodies. B . Testis protein extracts and buffer controls were incubated with anti-PP1γ2, anti-PPP1R11, or preimmune serum immobilized on Protein G-Sepharose-4 beads, as indicated at the top of the figure. The immunoprecipitates were separated by SDS-PAGE and immunoblotted for the proteins indicated at the bottom of the figure. (ND: not done).
    Gst Pull Down Assay Glutathione Sepharose 4b Beads, supplied by GE Healthcare, used in various techniques. Bioz Stars score: 79/100, based on 7 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/gst pull down assay glutathione sepharose 4b beads/product/GE Healthcare
    Average 79 stars, based on 7 article reviews
    Price from $9.99 to $1999.99
    gst pull down assay glutathione sepharose 4b beads - by Bioz Stars, 2020-01
    79/100 stars
      Buy from Supplier

    77
    GE Healthcare antigen coupled beads glutathione sepharose 4b beads
    Sds22, PPP1R11, and PP1γ2 are bound to each other in crude testis protein extracts. A . The recombinant proteins <t>GST-PPP1R11</t> and control GST were incubated with testis cell lysates in the presence of <t>Glutathione-Sepharose</t> beads. The eluted proteins and testis extracts alone were resolved by SDS-PAGE and subjected to western blot analysis with anti-Sds22 and anti-PP1γ2 antibodies. B . Testis protein extracts and buffer controls were incubated with anti-PP1γ2, anti-PPP1R11, or preimmune serum immobilized on Protein G-Sepharose-4 beads, as indicated at the top of the figure. The immunoprecipitates were separated by SDS-PAGE and immunoblotted for the proteins indicated at the bottom of the figure. (ND: not done).
    Antigen Coupled Beads Glutathione Sepharose 4b Beads, supplied by GE Healthcare, used in various techniques. Bioz Stars score: 77/100, based on 3 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/antigen coupled beads glutathione sepharose 4b beads/product/GE Healthcare
    Average 77 stars, based on 3 article reviews
    Price from $9.99 to $1999.99
    antigen coupled beads glutathione sepharose 4b beads - by Bioz Stars, 2020-01
    77/100 stars
      Buy from Supplier

    78
    GE Healthcare gamma glutathione sepharose beads
    Sds22, PPP1R11, and PP1γ2 are bound to each other in crude testis protein extracts. A . The recombinant proteins <t>GST-PPP1R11</t> and control GST were incubated with testis cell lysates in the presence of <t>Glutathione-Sepharose</t> beads. The eluted proteins and testis extracts alone were resolved by SDS-PAGE and subjected to western blot analysis with anti-Sds22 and anti-PP1γ2 antibodies. B . Testis protein extracts and buffer controls were incubated with anti-PP1γ2, anti-PPP1R11, or preimmune serum immobilized on Protein G-Sepharose-4 beads, as indicated at the top of the figure. The immunoprecipitates were separated by SDS-PAGE and immunoblotted for the proteins indicated at the bottom of the figure. (ND: not done).
    Gamma Glutathione Sepharose Beads, supplied by GE Healthcare, used in various techniques. Bioz Stars score: 78/100, based on 4 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/gamma glutathione sepharose beads/product/GE Healthcare
    Average 78 stars, based on 4 article reviews
    Price from $9.99 to $1999.99
    gamma glutathione sepharose beads - by Bioz Stars, 2020-01
    78/100 stars
      Buy from Supplier

    85
    GE Healthcare glutathione sepharose 4ff beads
    Sds22, PPP1R11, and PP1γ2 are bound to each other in crude testis protein extracts. A . The recombinant proteins <t>GST-PPP1R11</t> and control GST were incubated with testis cell lysates in the presence of <t>Glutathione-Sepharose</t> beads. The eluted proteins and testis extracts alone were resolved by SDS-PAGE and subjected to western blot analysis with anti-Sds22 and anti-PP1γ2 antibodies. B . Testis protein extracts and buffer controls were incubated with anti-PP1γ2, anti-PPP1R11, or preimmune serum immobilized on Protein G-Sepharose-4 beads, as indicated at the top of the figure. The immunoprecipitates were separated by SDS-PAGE and immunoblotted for the proteins indicated at the bottom of the figure. (ND: not done).
    Glutathione Sepharose 4ff Beads, supplied by GE Healthcare, used in various techniques. Bioz Stars score: 85/100, based on 25 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/glutathione sepharose 4ff beads/product/GE Healthcare
    Average 85 stars, based on 25 article reviews
    Price from $9.99 to $1999.99
    glutathione sepharose 4ff beads - by Bioz Stars, 2020-01
    85/100 stars
      Buy from Supplier

    97
    GE Healthcare glutathione coated sepharose beads
    Sds22, PPP1R11, and PP1γ2 are bound to each other in crude testis protein extracts. A . The recombinant proteins <t>GST-PPP1R11</t> and control GST were incubated with testis cell lysates in the presence of <t>Glutathione-Sepharose</t> beads. The eluted proteins and testis extracts alone were resolved by SDS-PAGE and subjected to western blot analysis with anti-Sds22 and anti-PP1γ2 antibodies. B . Testis protein extracts and buffer controls were incubated with anti-PP1γ2, anti-PPP1R11, or preimmune serum immobilized on Protein G-Sepharose-4 beads, as indicated at the top of the figure. The immunoprecipitates were separated by SDS-PAGE and immunoblotted for the proteins indicated at the bottom of the figure. (ND: not done).
    Glutathione Coated Sepharose Beads, supplied by GE Healthcare, used in various techniques. Bioz Stars score: 97/100, based on 76 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/glutathione coated sepharose beads/product/GE Healthcare
    Average 97 stars, based on 76 article reviews
    Price from $9.99 to $1999.99
    glutathione coated sepharose beads - by Bioz Stars, 2020-01
    97/100 stars
      Buy from Supplier

    80
    GE Healthcare glutathione sepharose 4g beads
    Sds22, PPP1R11, and PP1γ2 are bound to each other in crude testis protein extracts. A . The recombinant proteins <t>GST-PPP1R11</t> and control GST were incubated with testis cell lysates in the presence of <t>Glutathione-Sepharose</t> beads. The eluted proteins and testis extracts alone were resolved by SDS-PAGE and subjected to western blot analysis with anti-Sds22 and anti-PP1γ2 antibodies. B . Testis protein extracts and buffer controls were incubated with anti-PP1γ2, anti-PPP1R11, or preimmune serum immobilized on Protein G-Sepharose-4 beads, as indicated at the top of the figure. The immunoprecipitates were separated by SDS-PAGE and immunoblotted for the proteins indicated at the bottom of the figure. (ND: not done).
    Glutathione Sepharose 4g Beads, supplied by GE Healthcare, used in various techniques. Bioz Stars score: 80/100, based on 7 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/glutathione sepharose 4g beads/product/GE Healthcare
    Average 80 stars, based on 7 article reviews
    Price from $9.99 to $1999.99
    glutathione sepharose 4g beads - by Bioz Stars, 2020-01
    80/100 stars
      Buy from Supplier

    81
    GE Healthcare glutathione sepharose cl4b beads
    Sds22, PPP1R11, and PP1γ2 are bound to each other in crude testis protein extracts. A . The recombinant proteins <t>GST-PPP1R11</t> and control GST were incubated with testis cell lysates in the presence of <t>Glutathione-Sepharose</t> beads. The eluted proteins and testis extracts alone were resolved by SDS-PAGE and subjected to western blot analysis with anti-Sds22 and anti-PP1γ2 antibodies. B . Testis protein extracts and buffer controls were incubated with anti-PP1γ2, anti-PPP1R11, or preimmune serum immobilized on Protein G-Sepharose-4 beads, as indicated at the top of the figure. The immunoprecipitates were separated by SDS-PAGE and immunoblotted for the proteins indicated at the bottom of the figure. (ND: not done).
    Glutathione Sepharose Cl4b Beads, supplied by GE Healthcare, used in various techniques. Bioz Stars score: 81/100, based on 12 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/glutathione sepharose cl4b beads/product/GE Healthcare
    Average 81 stars, based on 12 article reviews
    Price from $9.99 to $1999.99
    glutathione sepharose cl4b beads - by Bioz Stars, 2020-01
    81/100 stars
      Buy from Supplier

    79
    GE Healthcare prepared glutathione sepharose beads
    Sds22, PPP1R11, and PP1γ2 are bound to each other in crude testis protein extracts. A . The recombinant proteins <t>GST-PPP1R11</t> and control GST were incubated with testis cell lysates in the presence of <t>Glutathione-Sepharose</t> beads. The eluted proteins and testis extracts alone were resolved by SDS-PAGE and subjected to western blot analysis with anti-Sds22 and anti-PP1γ2 antibodies. B . Testis protein extracts and buffer controls were incubated with anti-PP1γ2, anti-PPP1R11, or preimmune serum immobilized on Protein G-Sepharose-4 beads, as indicated at the top of the figure. The immunoprecipitates were separated by SDS-PAGE and immunoblotted for the proteins indicated at the bottom of the figure. (ND: not done).
    Prepared Glutathione Sepharose Beads, supplied by GE Healthcare, used in various techniques. Bioz Stars score: 79/100, based on 9 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/prepared glutathione sepharose beads/product/GE Healthcare
    Average 79 stars, based on 9 article reviews
    Price from $9.99 to $1999.99
    prepared glutathione sepharose beads - by Bioz Stars, 2020-01
    79/100 stars
      Buy from Supplier

    82
    GE Healthcare glutathione sepharose 6b beads
    Sds22, PPP1R11, and PP1γ2 are bound to each other in crude testis protein extracts. A . The recombinant proteins <t>GST-PPP1R11</t> and control GST were incubated with testis cell lysates in the presence of <t>Glutathione-Sepharose</t> beads. The eluted proteins and testis extracts alone were resolved by SDS-PAGE and subjected to western blot analysis with anti-Sds22 and anti-PP1γ2 antibodies. B . Testis protein extracts and buffer controls were incubated with anti-PP1γ2, anti-PPP1R11, or preimmune serum immobilized on Protein G-Sepharose-4 beads, as indicated at the top of the figure. The immunoprecipitates were separated by SDS-PAGE and immunoblotted for the proteins indicated at the bottom of the figure. (ND: not done).
    Glutathione Sepharose 6b Beads, supplied by GE Healthcare, used in various techniques. Bioz Stars score: 82/100, based on 13 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/glutathione sepharose 6b beads/product/GE Healthcare
    Average 82 stars, based on 13 article reviews
    Price from $9.99 to $1999.99
    glutathione sepharose 6b beads - by Bioz Stars, 2020-01
    82/100 stars
      Buy from Supplier

    70
    GE Healthcare glutathione sepharose tm4b beads
    Sds22, PPP1R11, and PP1γ2 are bound to each other in crude testis protein extracts. A . The recombinant proteins <t>GST-PPP1R11</t> and control GST were incubated with testis cell lysates in the presence of <t>Glutathione-Sepharose</t> beads. The eluted proteins and testis extracts alone were resolved by SDS-PAGE and subjected to western blot analysis with anti-Sds22 and anti-PP1γ2 antibodies. B . Testis protein extracts and buffer controls were incubated with anti-PP1γ2, anti-PPP1R11, or preimmune serum immobilized on Protein G-Sepharose-4 beads, as indicated at the top of the figure. The immunoprecipitates were separated by SDS-PAGE and immunoblotted for the proteins indicated at the bottom of the figure. (ND: not done).
    Glutathione Sepharose Tm4b Beads, supplied by GE Healthcare, used in various techniques. Bioz Stars score: 70/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/glutathione sepharose tm4b beads/product/GE Healthcare
    Average 70 stars, based on 1 article reviews
    Price from $9.99 to $1999.99
    glutathione sepharose tm4b beads - by Bioz Stars, 2020-01
    70/100 stars
      Buy from Supplier

    70
    GE Healthcare prebounded glutathione sepharose beads
    Sds22, PPP1R11, and PP1γ2 are bound to each other in crude testis protein extracts. A . The recombinant proteins <t>GST-PPP1R11</t> and control GST were incubated with testis cell lysates in the presence of <t>Glutathione-Sepharose</t> beads. The eluted proteins and testis extracts alone were resolved by SDS-PAGE and subjected to western blot analysis with anti-Sds22 and anti-PP1γ2 antibodies. B . Testis protein extracts and buffer controls were incubated with anti-PP1γ2, anti-PPP1R11, or preimmune serum immobilized on Protein G-Sepharose-4 beads, as indicated at the top of the figure. The immunoprecipitates were separated by SDS-PAGE and immunoblotted for the proteins indicated at the bottom of the figure. (ND: not done).
    Prebounded Glutathione Sepharose Beads, supplied by GE Healthcare, used in various techniques. Bioz Stars score: 70/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/prebounded glutathione sepharose beads/product/GE Healthcare
    Average 70 stars, based on 1 article reviews
    Price from $9.99 to $1999.99
    prebounded glutathione sepharose beads - by Bioz Stars, 2020-01
    70/100 stars
      Buy from Supplier

    79
    GE Healthcare glutathione sepharose gth beads
    Mechanism of D-loop formation by HLTF. ( A ) Self-association of HLTF. GST pull-down experiment was carried out using FLAG-HLTF and GST-HLTF or GST followed by immobilization on <t>GTH</t> beads. The beads were washed, bound proteins were eluted and reactions were analysed on 10% SDS–PAGE gels. ( B ) Western blot analyses of the GST pull-down experiment. We sequentially developed the filter by anti-FLAG, anti-GST and finally anti-HLTF antibodies as indicated on the bottom of the gels together with the bands corresponding to individual proteins. ( C ) HLTF mediates change in DNA conformation in ATP-dependent manner. Increasing amounts of HLTF (22, 66 and 200 nM) were incubated with topologically relaxed DNA and topoisomerase I with (lanes 2–5) or without (lanes 6–8) ATP as indicated. Lane 1 represents control reaction in the absence of topoisomerase I. All reactions were stopped by addition of SDS/proteinase K and reaction products resolved on 0.8% native <t>agarose</t> gel. Position of Relaxed and Form U DNA is indicated.
    Glutathione Sepharose Gth Beads, supplied by GE Healthcare, used in various techniques. Bioz Stars score: 79/100, based on 5 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/glutathione sepharose gth beads/product/GE Healthcare
    Average 79 stars, based on 5 article reviews
    Price from $9.99 to $1999.99
    glutathione sepharose gth beads - by Bioz Stars, 2020-01
    79/100 stars
      Buy from Supplier

    Image Search Results


    TBK1 phosphorylates RKIP at serine 109 BMDM cells were infected with VSV (MOI 0.1) or HSV (MOI 5) for the indicated times. Cell lysates were analyzed by immunoblotting with the indicated antibodies. BMDM cells were transfected with control or TBK1 siRNA. 72 h after transfection, cells were analyzed by immunoblotting with antibodies for phospho‐RKIP or TBK1. Immunoblot analysis of RKIP phosphorylation in control or TBK1 −/− mouse embryonic fibroblasts after VSV (MOI 0.1) infection for the indicated times. Recombinant flag‐tagged TBK1 was immunoprecipitated with anti‐flag M2 beads in flag‐TBK1‐transfected 293T cells. Followed this, in vitro kinase assay was carried out in the reaction mixture containing 2 mM ATP, 100 μM GST‐RKIP, or 100 μM GST‐GFP together with 5 μM flag‐TBK1 at 30°C for 30 min. Assay mixtures were immunoblotted with the indicated antibodies for phospho‐RKIP, flag, GST, or his. Immunoblot analysis of extracts of RAW264.7 cells stably overexpressing flag‐RKIP, flag‐S109A, and flag‐S109D, and infected with VSV (MOI 0.1) for the indicated times, followed by immunoprecipitation with anti‐flag beads. BMDM cells were infected with VSV (MOI 0.1) for the indicated times. The cell lysates were immunoprecipitated with anti‐TBK1 (left) or anti‐RKIP (right). The immunoprecipitates were analyzed by immunoblot with anti‐RKIP or anti‐TBK1 antibody. The levels of the endogenous RKIP, TBK1, and p‐RKIP were detected by immunoblot analysis. Co‐immunoprecipitation and immunoblot analysis of 293T cells transfected with myc‐TBK1 alone or with flag‐RKIP, flag‐S109A, or flag‐S109D. GST pull‐down experiment was performed with 1 μg fusion protein GST‐GFP, GST‐his‐RKIP, or GST‐his‐S109D mixed with 20 μl pre‐cleared agarose beads in 800 μl reaction medium, followed by adding 1 μg flag‐TBK1 and incubating at 4°C for 3 h with gentle rotation. Pull‐down (lane 1–3) and input samples (lane 4) were separated by SDS–PAGE followed by immunoblotting with anti‐GST or anti‐flag antibody. Immunoprecipitation and immunoblot analysis of 293T cells transfected with indicated combinations of vector for myc‐TBK1, flag‐RKIP, flag‐S109A, flag‐S109D, HA‐GSK3β or flag‐GFP. In vitro assay was carried out in the reaction mixture containing 2 mM ATP, 50 μM GST‐TBK1 together with 100 μM GST‐his‐RKIP, 100 μM GST‐his‐S109D, 100 μM GST‐his‐S109A, or 100 μM GST, respectively, at 30°C for 30 min, followed by immunoblotting with anti‐p‐TBK1 or anti‐GST antibody. . Source data are available online for this figure.

    Journal: The EMBO Journal

    Article Title: RKIP and TBK1 form a positive feedback loop to promote type I interferon production in innate immunity

    doi: 10.15252/embj.201694060

    Figure Lengend Snippet: TBK1 phosphorylates RKIP at serine 109 BMDM cells were infected with VSV (MOI 0.1) or HSV (MOI 5) for the indicated times. Cell lysates were analyzed by immunoblotting with the indicated antibodies. BMDM cells were transfected with control or TBK1 siRNA. 72 h after transfection, cells were analyzed by immunoblotting with antibodies for phospho‐RKIP or TBK1. Immunoblot analysis of RKIP phosphorylation in control or TBK1 −/− mouse embryonic fibroblasts after VSV (MOI 0.1) infection for the indicated times. Recombinant flag‐tagged TBK1 was immunoprecipitated with anti‐flag M2 beads in flag‐TBK1‐transfected 293T cells. Followed this, in vitro kinase assay was carried out in the reaction mixture containing 2 mM ATP, 100 μM GST‐RKIP, or 100 μM GST‐GFP together with 5 μM flag‐TBK1 at 30°C for 30 min. Assay mixtures were immunoblotted with the indicated antibodies for phospho‐RKIP, flag, GST, or his. Immunoblot analysis of extracts of RAW264.7 cells stably overexpressing flag‐RKIP, flag‐S109A, and flag‐S109D, and infected with VSV (MOI 0.1) for the indicated times, followed by immunoprecipitation with anti‐flag beads. BMDM cells were infected with VSV (MOI 0.1) for the indicated times. The cell lysates were immunoprecipitated with anti‐TBK1 (left) or anti‐RKIP (right). The immunoprecipitates were analyzed by immunoblot with anti‐RKIP or anti‐TBK1 antibody. The levels of the endogenous RKIP, TBK1, and p‐RKIP were detected by immunoblot analysis. Co‐immunoprecipitation and immunoblot analysis of 293T cells transfected with myc‐TBK1 alone or with flag‐RKIP, flag‐S109A, or flag‐S109D. GST pull‐down experiment was performed with 1 μg fusion protein GST‐GFP, GST‐his‐RKIP, or GST‐his‐S109D mixed with 20 μl pre‐cleared agarose beads in 800 μl reaction medium, followed by adding 1 μg flag‐TBK1 and incubating at 4°C for 3 h with gentle rotation. Pull‐down (lane 1–3) and input samples (lane 4) were separated by SDS–PAGE followed by immunoblotting with anti‐GST or anti‐flag antibody. Immunoprecipitation and immunoblot analysis of 293T cells transfected with indicated combinations of vector for myc‐TBK1, flag‐RKIP, flag‐S109A, flag‐S109D, HA‐GSK3β or flag‐GFP. In vitro assay was carried out in the reaction mixture containing 2 mM ATP, 50 μM GST‐TBK1 together with 100 μM GST‐his‐RKIP, 100 μM GST‐his‐S109D, 100 μM GST‐his‐S109A, or 100 μM GST, respectively, at 30°C for 30 min, followed by immunoblotting with anti‐p‐TBK1 or anti‐GST antibody. . Source data are available online for this figure.

    Article Snippet: The fusion proteins of GST‐GFP, GST‐his‐RKIP, and GST‐his‐S109D were expressed in E. coli and purified according to standard protocols with pre‐equilibrated glutathione–Sepharose beads (GE Healthcare).

    Techniques: Infection, Transfection, Recombinant, Immunoprecipitation, In Vitro, Kinase Assay, Stable Transfection, SDS Page, Plasmid Preparation

    Interaction of Siah2 with PHD3. ( a ) HEK293 cells were transfected in 60-mm cell culture plates for 2 days with the indicated expression plasmids. The cells were lysed, and the lysates were subjected to FLAG immunoprecipitation (IP), as described under “ Materials and Methods ”. Aliquots of the cell lysates and immunoprecipitates were analyzed by western blotting with the anti-HA antibody. Both full length Siah2 and Siah2 SBD bind to PHD3 to the same extent. In the IP, the presence of the faint band in the empty vector lane is due to non-specific binding of PHD3. The same membrane was reblotted with FLAG antibody to detect FLAG tagged Siah2 proteins. ( b ) GST-Siah2 SBD pulldown of HA-PHD3. Cell lysate of HEK293 cells transfected with HA-PHD3 was incubated with GST-Siah2 SBD immobilized on GSH agarose beads and the reaction was performed as described under “ Material and Methods ”. The empty expression vector alone was expressed as a GST control for non-specific binding of HA PHD3. After the incubation, the lysate was removed, the GSH-agarose beads were washed, and bound HA-PHD3 was analyzed by Western blotting using anti HA antibody. The pull down assay confirmed the interaction of Siah2 SBD with PHD3.

    Journal: PLoS ONE

    Article Title: Investigating the Molecular Basis of Siah1 and Siah2 E3 Ubiquitin Ligase Substrate Specificity

    doi: 10.1371/journal.pone.0106547

    Figure Lengend Snippet: Interaction of Siah2 with PHD3. ( a ) HEK293 cells were transfected in 60-mm cell culture plates for 2 days with the indicated expression plasmids. The cells were lysed, and the lysates were subjected to FLAG immunoprecipitation (IP), as described under “ Materials and Methods ”. Aliquots of the cell lysates and immunoprecipitates were analyzed by western blotting with the anti-HA antibody. Both full length Siah2 and Siah2 SBD bind to PHD3 to the same extent. In the IP, the presence of the faint band in the empty vector lane is due to non-specific binding of PHD3. The same membrane was reblotted with FLAG antibody to detect FLAG tagged Siah2 proteins. ( b ) GST-Siah2 SBD pulldown of HA-PHD3. Cell lysate of HEK293 cells transfected with HA-PHD3 was incubated with GST-Siah2 SBD immobilized on GSH agarose beads and the reaction was performed as described under “ Material and Methods ”. The empty expression vector alone was expressed as a GST control for non-specific binding of HA PHD3. After the incubation, the lysate was removed, the GSH-agarose beads were washed, and bound HA-PHD3 was analyzed by Western blotting using anti HA antibody. The pull down assay confirmed the interaction of Siah2 SBD with PHD3.

    Article Snippet: GST pull down For GST pull down assay, GST-Siah2 SBD was allowed to bind to glutathione sepharose beads (GSH) (GE Healthcare) for 30 min at 4°C in binding buffer containing 50 mM Tris-HCl (pH 8), 150 mM NaCl, 1 mM DTT, 5% glycerol, 0.1% Triton X-100.

    Techniques: Transfection, Cell Culture, Expressing, Immunoprecipitation, Western Blot, Plasmid Preparation, Binding Assay, Incubation, Pull Down Assay

    The 2BC non-structural protein of EV-A71 interacts with syntaxin-17 (STX17) and synaptosome-associated protein of 29 kDa (SNAP29). ( A ) Yeast two-hybrid assays of 2BC and STX17. Yeasts were transformed with pGBKT7 or pGBKT7-STX17 together with pACT2 or pACT2–2BC. Yeasts were then grown on selective medium without tryptophan (-W) or without leucine, tryptophan, and histidine (-LWH); ( B ) HeLa cells were transfected with pDEST27 or pDEST27–2BC in combination with pCherry-STX17 for 48 h. The transfected cells were examined under a confocal microscope with objective 63× (scale bar 10µm) for the presence of co-localization; and ( C ) the Pearson’s correlation coefficient was determined; ( D ) HEK-293T cells were co-transfected with FLAG-STX17 and GST-2BC for 48 h. Lysates were collected for co-immunoprecipitation (IP) using glutathione (GST) sepharose. Western Blot was performed to detect FLAG-STX17 and GST fusion proteins; ( E ) RD cells were infected with EV-A71 for 8 hpi and lysates of infected cells were then harvested prior to co-immunoprecipitation (co-IP) assay using anti-STX17 and anti-SNAP29. The final IP eluates and discarded IP flow through were collected for WB to detect 2BC (anti-2C polyclonal antibody) and structural proteins (mAB979) of EV-A71. One-way ANOVA: * p

    Journal: Viruses

    Article Title: 2BC Non-Structural Protein of Enterovirus A71 Interacts with SNARE Proteins to Trigger Autolysosome Formation

    doi: 10.3390/v9070169

    Figure Lengend Snippet: The 2BC non-structural protein of EV-A71 interacts with syntaxin-17 (STX17) and synaptosome-associated protein of 29 kDa (SNAP29). ( A ) Yeast two-hybrid assays of 2BC and STX17. Yeasts were transformed with pGBKT7 or pGBKT7-STX17 together with pACT2 or pACT2–2BC. Yeasts were then grown on selective medium without tryptophan (-W) or without leucine, tryptophan, and histidine (-LWH); ( B ) HeLa cells were transfected with pDEST27 or pDEST27–2BC in combination with pCherry-STX17 for 48 h. The transfected cells were examined under a confocal microscope with objective 63× (scale bar 10µm) for the presence of co-localization; and ( C ) the Pearson’s correlation coefficient was determined; ( D ) HEK-293T cells were co-transfected with FLAG-STX17 and GST-2BC for 48 h. Lysates were collected for co-immunoprecipitation (IP) using glutathione (GST) sepharose. Western Blot was performed to detect FLAG-STX17 and GST fusion proteins; ( E ) RD cells were infected with EV-A71 for 8 hpi and lysates of infected cells were then harvested prior to co-immunoprecipitation (co-IP) assay using anti-STX17 and anti-SNAP29. The final IP eluates and discarded IP flow through were collected for WB to detect 2BC (anti-2C polyclonal antibody) and structural proteins (mAB979) of EV-A71. One-way ANOVA: * p

    Article Snippet: Co-Affinity Purification (Co-AP) Each expression vector (1.5 µg) was transfected into HEK293T cells for 48 h. After cell lysis, Glutathione (GST)-sepharose 4B beads (GE Healthcare, Chalfont St Giles, UK) were used for the co-affinity purification (co-AP) [ ].

    Techniques: Transformation Assay, Transfection, Microscopy, Immunoprecipitation, Western Blot, Infection, Co-Immunoprecipitation Assay, Flow Cytometry

    Pull-down assays of NtWRKY12, TGA1a, TGA2.1, TGA2.2, and NtNPR1 . GST-proteins were incubated with Strep/HIS purified fusion proteins and complexes were pulled down with Streptactin–Sepharose beads (A) or Glutathione–Sepharose 4B beads (B,C) . After SDS-PAGE and Western blotting fusion proteins were detected with anti-GST antibodies (A) or anti-HIS antibodies (B,C) . Plus and minus signs denote the presence or absence in the incubation mixtures of the proteins indicated at the left. The input protein was loaded separately on gel and is indicated by (I). The table in (D) summarizes the results of the pull-down assays. Plus-sign, interaction, minus-sign, no interaction, N.D., not determined.

    Journal: Frontiers in plant science

    Article Title: Tobacco Transcription Factor NtWRKY12 Interacts with TGA2.2 in vitro and in vivo

    doi: 10.3389/fpls.2011.00032

    Figure Lengend Snippet: Pull-down assays of NtWRKY12, TGA1a, TGA2.1, TGA2.2, and NtNPR1 . GST-proteins were incubated with Strep/HIS purified fusion proteins and complexes were pulled down with Streptactin–Sepharose beads (A) or Glutathione–Sepharose 4B beads (B,C) . After SDS-PAGE and Western blotting fusion proteins were detected with anti-GST antibodies (A) or anti-HIS antibodies (B,C) . Plus and minus signs denote the presence or absence in the incubation mixtures of the proteins indicated at the left. The input protein was loaded separately on gel and is indicated by (I). The table in (D) summarizes the results of the pull-down assays. Plus-sign, interaction, minus-sign, no interaction, N.D., not determined.

    Article Snippet: To this mixture Glutathione–Sepharose 4B beads (GE Healthcare) or Strep–Tactin Sepharose beads (IBA) in buffer W (100 mM Tris pH 8.0, 150 mM NaCl, 1 mM EDTA) were added, and incubation was continued for an additional hour.

    Techniques: Incubation, Purification, SDS Page, Western Blot

    Sds22, PPP1R11, and PP1γ2 are bound to each other in crude testis protein extracts. A . The recombinant proteins GST-PPP1R11 and control GST were incubated with testis cell lysates in the presence of Glutathione-Sepharose beads. The eluted proteins and testis extracts alone were resolved by SDS-PAGE and subjected to western blot analysis with anti-Sds22 and anti-PP1γ2 antibodies. B . Testis protein extracts and buffer controls were incubated with anti-PP1γ2, anti-PPP1R11, or preimmune serum immobilized on Protein G-Sepharose-4 beads, as indicated at the top of the figure. The immunoprecipitates were separated by SDS-PAGE and immunoblotted for the proteins indicated at the bottom of the figure. (ND: not done).

    Journal: PLoS ONE

    Article Title: PP1?2 and PPP1R11 Are Parts of a Multimeric Complex in Developing Testicular Germ Cells in which their Steady State Levels Are Reciprocally Related

    doi: 10.1371/journal.pone.0004861

    Figure Lengend Snippet: Sds22, PPP1R11, and PP1γ2 are bound to each other in crude testis protein extracts. A . The recombinant proteins GST-PPP1R11 and control GST were incubated with testis cell lysates in the presence of Glutathione-Sepharose beads. The eluted proteins and testis extracts alone were resolved by SDS-PAGE and subjected to western blot analysis with anti-Sds22 and anti-PP1γ2 antibodies. B . Testis protein extracts and buffer controls were incubated with anti-PP1γ2, anti-PPP1R11, or preimmune serum immobilized on Protein G-Sepharose-4 beads, as indicated at the top of the figure. The immunoprecipitates were separated by SDS-PAGE and immunoblotted for the proteins indicated at the bottom of the figure. (ND: not done).

    Article Snippet: GST Pull-Down Assay Glutathione Sepharose 4B beads (GE Healthcare, Piscataway, NJ, USA) bound to GST-PPP1R11 or GST alone (as a negative control) were incubated with mouse testis extracts with rocking for 2 h at 4°C.

    Techniques: Recombinant, Incubation, SDS Page, Western Blot

    Mechanism of D-loop formation by HLTF. ( A ) Self-association of HLTF. GST pull-down experiment was carried out using FLAG-HLTF and GST-HLTF or GST followed by immobilization on GTH beads. The beads were washed, bound proteins were eluted and reactions were analysed on 10% SDS–PAGE gels. ( B ) Western blot analyses of the GST pull-down experiment. We sequentially developed the filter by anti-FLAG, anti-GST and finally anti-HLTF antibodies as indicated on the bottom of the gels together with the bands corresponding to individual proteins. ( C ) HLTF mediates change in DNA conformation in ATP-dependent manner. Increasing amounts of HLTF (22, 66 and 200 nM) were incubated with topologically relaxed DNA and topoisomerase I with (lanes 2–5) or without (lanes 6–8) ATP as indicated. Lane 1 represents control reaction in the absence of topoisomerase I. All reactions were stopped by addition of SDS/proteinase K and reaction products resolved on 0.8% native agarose gel. Position of Relaxed and Form U DNA is indicated.

    Journal: Nucleic Acids Research

    Article Title: Strand invasion by HLTF as a mechanism for template switch in fork rescue

    doi: 10.1093/nar/gkt1040

    Figure Lengend Snippet: Mechanism of D-loop formation by HLTF. ( A ) Self-association of HLTF. GST pull-down experiment was carried out using FLAG-HLTF and GST-HLTF or GST followed by immobilization on GTH beads. The beads were washed, bound proteins were eluted and reactions were analysed on 10% SDS–PAGE gels. ( B ) Western blot analyses of the GST pull-down experiment. We sequentially developed the filter by anti-FLAG, anti-GST and finally anti-HLTF antibodies as indicated on the bottom of the gels together with the bands corresponding to individual proteins. ( C ) HLTF mediates change in DNA conformation in ATP-dependent manner. Increasing amounts of HLTF (22, 66 and 200 nM) were incubated with topologically relaxed DNA and topoisomerase I with (lanes 2–5) or without (lanes 6–8) ATP as indicated. Lane 1 represents control reaction in the absence of topoisomerase I. All reactions were stopped by addition of SDS/proteinase K and reaction products resolved on 0.8% native agarose gel. Position of Relaxed and Form U DNA is indicated.

    Article Snippet: GST pull-down experiment Purified GST or GST-HLTF proteins (3 μg) were incubated with Glutathione-Sepharose (GTH) beads (GE Healthcare) for 4 h at 4°C with FLAG-HLTF (1 μg) in buffer E (40 mM Tris–HCl, pH 7.5, 100 mM NaCl, 0.1 mM DTT, 10% glycerol, 0.01% NP40).

    Techniques: SDS Page, Western Blot, Incubation, Agarose Gel Electrophoresis