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  • 99
    Thermo Fisher dmem glutamaxtm
    Dmem Glutamaxtm, supplied by Thermo Fisher, used in various techniques. Bioz Stars score: 99/100, based on 137 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    Thermo Fisher glutamaxtm
    Glutamaxtm, supplied by Thermo Fisher, used in various techniques. Bioz Stars score: 93/100, based on 1245 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    Thermo Fisher glutamaxtm i
    Glutamaxtm I, supplied by Thermo Fisher, used in various techniques. Bioz Stars score: 89/100, based on 117 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    Thermo Fisher rpmi 1640 glutamaxtm
    Rpmi 1640 Glutamaxtm, supplied by Thermo Fisher, used in various techniques. Bioz Stars score: 99/100, based on 63 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    Thermo Fisher dmem f12 glutamaxtm
    MUC20 knockdown inhibits HGF/MET signalling in PDAC cells. a MUC20 knockdown decreased phospho-MET levels. MUC20 was knocked down with siRNA (si-MUC20-1) in HPAF-II cells cultured with PSC-conditioned medium. Changes in 49 phospho-RTKs were analysed by western blotting of human phospho-RTK array (R D Systems). b MUC20 knockdown inhibited phosphorylation of MET and AKT in HPAC and HPAF-II cells treated with 25 ng/ml HGF. c MUC20 knockdown inhibited HGF-triggered migration and invasion in HPAC and HPAF-II cells analysed by Transwell migration assay and Matrigel invasion assay, respectively. Scale bars indicate 1 mm. d MUC20 knockdown significantly reversed HGF-enhanced viability in HPAC and HPAF-II cells. Cells cultured in 10% <t>FBS/DMEM-F12</t> were treated with PBS, MET inhibitor PHA665752 (2.5 µM) or HGF (25 ng/ml), as indicated. Viability was analysed by the MTT assay. Data are presented as mean ( n = 3) ± SD. * P
    Dmem F12 Glutamaxtm, supplied by Thermo Fisher, used in various techniques. Bioz Stars score: 99/100, based on 32 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    Thermo Fisher dmem f 12 glutamax supplement
    MUC20 knockdown inhibits HGF/MET signalling in PDAC cells. a MUC20 knockdown decreased phospho-MET levels. MUC20 was knocked down with siRNA (si-MUC20-1) in HPAF-II cells cultured with PSC-conditioned medium. Changes in 49 phospho-RTKs were analysed by western blotting of human phospho-RTK array (R D Systems). b MUC20 knockdown inhibited phosphorylation of MET and AKT in HPAC and HPAF-II cells treated with 25 ng/ml HGF. c MUC20 knockdown inhibited HGF-triggered migration and invasion in HPAC and HPAF-II cells analysed by Transwell migration assay and Matrigel invasion assay, respectively. Scale bars indicate 1 mm. d MUC20 knockdown significantly reversed HGF-enhanced viability in HPAC and HPAF-II cells. Cells cultured in 10% <t>FBS/DMEM-F12</t> were treated with PBS, MET inhibitor PHA665752 (2.5 µM) or HGF (25 ng/ml), as indicated. Viability was analysed by the MTT assay. Data are presented as mean ( n = 3) ± SD. * P
    Dmem F 12 Glutamax Supplement, supplied by Thermo Fisher, used in various techniques. Bioz Stars score: 99/100, based on 335 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    Thermo Fisher glutamaxtm 1
    MUC20 knockdown inhibits HGF/MET signalling in PDAC cells. a MUC20 knockdown decreased phospho-MET levels. MUC20 was knocked down with siRNA (si-MUC20-1) in HPAF-II cells cultured with PSC-conditioned medium. Changes in 49 phospho-RTKs were analysed by western blotting of human phospho-RTK array (R D Systems). b MUC20 knockdown inhibited phosphorylation of MET and AKT in HPAC and HPAF-II cells treated with 25 ng/ml HGF. c MUC20 knockdown inhibited HGF-triggered migration and invasion in HPAC and HPAF-II cells analysed by Transwell migration assay and Matrigel invasion assay, respectively. Scale bars indicate 1 mm. d MUC20 knockdown significantly reversed HGF-enhanced viability in HPAC and HPAF-II cells. Cells cultured in 10% <t>FBS/DMEM-F12</t> were treated with PBS, MET inhibitor PHA665752 (2.5 µM) or HGF (25 ng/ml), as indicated. Viability was analysed by the MTT assay. Data are presented as mean ( n = 3) ± SD. * P
    Glutamaxtm 1, supplied by Thermo Fisher, used in various techniques. Bioz Stars score: 88/100, based on 52 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    Thermo Fisher dulbecco s modified eagle s medium glutamaxtm
    MUC20 knockdown inhibits HGF/MET signalling in PDAC cells. a MUC20 knockdown decreased phospho-MET levels. MUC20 was knocked down with siRNA (si-MUC20-1) in HPAF-II cells cultured with PSC-conditioned medium. Changes in 49 phospho-RTKs were analysed by western blotting of human phospho-RTK array (R D Systems). b MUC20 knockdown inhibited phosphorylation of MET and AKT in HPAC and HPAF-II cells treated with 25 ng/ml HGF. c MUC20 knockdown inhibited HGF-triggered migration and invasion in HPAC and HPAF-II cells analysed by Transwell migration assay and Matrigel invasion assay, respectively. Scale bars indicate 1 mm. d MUC20 knockdown significantly reversed HGF-enhanced viability in HPAC and HPAF-II cells. Cells cultured in 10% <t>FBS/DMEM-F12</t> were treated with PBS, MET inhibitor PHA665752 (2.5 µM) or HGF (25 ng/ml), as indicated. Viability was analysed by the MTT assay. Data are presented as mean ( n = 3) ± SD. * P
    Dulbecco S Modified Eagle S Medium Glutamaxtm, supplied by Thermo Fisher, used in various techniques. Bioz Stars score: 99/100, based on 15 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    Thermo Fisher neurobasaltm b27 glutamaxtm
    MUC20 knockdown inhibits HGF/MET signalling in PDAC cells. a MUC20 knockdown decreased phospho-MET levels. MUC20 was knocked down with siRNA (si-MUC20-1) in HPAF-II cells cultured with PSC-conditioned medium. Changes in 49 phospho-RTKs were analysed by western blotting of human phospho-RTK array (R D Systems). b MUC20 knockdown inhibited phosphorylation of MET and AKT in HPAC and HPAF-II cells treated with 25 ng/ml HGF. c MUC20 knockdown inhibited HGF-triggered migration and invasion in HPAC and HPAF-II cells analysed by Transwell migration assay and Matrigel invasion assay, respectively. Scale bars indicate 1 mm. d MUC20 knockdown significantly reversed HGF-enhanced viability in HPAC and HPAF-II cells. Cells cultured in 10% <t>FBS/DMEM-F12</t> were treated with PBS, MET inhibitor PHA665752 (2.5 µM) or HGF (25 ng/ml), as indicated. Viability was analysed by the MTT assay. Data are presented as mean ( n = 3) ± SD. * P
    Neurobasaltm B27 Glutamaxtm, supplied by Thermo Fisher, used in various techniques. Bioz Stars score: 99/100, based on 9 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    Thermo Fisher imdm glutamaxtm
    MUC20 knockdown inhibits HGF/MET signalling in PDAC cells. a MUC20 knockdown decreased phospho-MET levels. MUC20 was knocked down with siRNA (si-MUC20-1) in HPAF-II cells cultured with PSC-conditioned medium. Changes in 49 phospho-RTKs were analysed by western blotting of human phospho-RTK array (R D Systems). b MUC20 knockdown inhibited phosphorylation of MET and AKT in HPAC and HPAF-II cells treated with 25 ng/ml HGF. c MUC20 knockdown inhibited HGF-triggered migration and invasion in HPAC and HPAF-II cells analysed by Transwell migration assay and Matrigel invasion assay, respectively. Scale bars indicate 1 mm. d MUC20 knockdown significantly reversed HGF-enhanced viability in HPAC and HPAF-II cells. Cells cultured in 10% <t>FBS/DMEM-F12</t> were treated with PBS, MET inhibitor PHA665752 (2.5 µM) or HGF (25 ng/ml), as indicated. Viability was analysed by the MTT assay. Data are presented as mean ( n = 3) ± SD. * P
    Imdm Glutamaxtm, supplied by Thermo Fisher, used in various techniques. Bioz Stars score: 99/100, based on 5 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    Thermo Fisher dmem glutamaxtm i medium
    MUC20 knockdown inhibits HGF/MET signalling in PDAC cells. a MUC20 knockdown decreased phospho-MET levels. MUC20 was knocked down with siRNA (si-MUC20-1) in HPAF-II cells cultured with PSC-conditioned medium. Changes in 49 phospho-RTKs were analysed by western blotting of human phospho-RTK array (R D Systems). b MUC20 knockdown inhibited phosphorylation of MET and AKT in HPAC and HPAF-II cells treated with 25 ng/ml HGF. c MUC20 knockdown inhibited HGF-triggered migration and invasion in HPAC and HPAF-II cells analysed by Transwell migration assay and Matrigel invasion assay, respectively. Scale bars indicate 1 mm. d MUC20 knockdown significantly reversed HGF-enhanced viability in HPAC and HPAF-II cells. Cells cultured in 10% <t>FBS/DMEM-F12</t> were treated with PBS, MET inhibitor PHA665752 (2.5 µM) or HGF (25 ng/ml), as indicated. Viability was analysed by the MTT assay. Data are presented as mean ( n = 3) ± SD. * P
    Dmem Glutamaxtm I Medium, supplied by Thermo Fisher, used in various techniques. Bioz Stars score: 99/100, based on 41 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    Image Search Results


    MUC20 knockdown inhibits HGF/MET signalling in PDAC cells. a MUC20 knockdown decreased phospho-MET levels. MUC20 was knocked down with siRNA (si-MUC20-1) in HPAF-II cells cultured with PSC-conditioned medium. Changes in 49 phospho-RTKs were analysed by western blotting of human phospho-RTK array (R D Systems). b MUC20 knockdown inhibited phosphorylation of MET and AKT in HPAC and HPAF-II cells treated with 25 ng/ml HGF. c MUC20 knockdown inhibited HGF-triggered migration and invasion in HPAC and HPAF-II cells analysed by Transwell migration assay and Matrigel invasion assay, respectively. Scale bars indicate 1 mm. d MUC20 knockdown significantly reversed HGF-enhanced viability in HPAC and HPAF-II cells. Cells cultured in 10% FBS/DMEM-F12 were treated with PBS, MET inhibitor PHA665752 (2.5 µM) or HGF (25 ng/ml), as indicated. Viability was analysed by the MTT assay. Data are presented as mean ( n = 3) ± SD. * P

    Journal: Oncogene

    Article Title: Silencing of MUC20 suppresses the malignant character of pancreatic ductal adenocarcinoma cells through inhibition of the HGF/MET pathway

    doi: 10.1038/s41388-018-0403-0

    Figure Lengend Snippet: MUC20 knockdown inhibits HGF/MET signalling in PDAC cells. a MUC20 knockdown decreased phospho-MET levels. MUC20 was knocked down with siRNA (si-MUC20-1) in HPAF-II cells cultured with PSC-conditioned medium. Changes in 49 phospho-RTKs were analysed by western blotting of human phospho-RTK array (R D Systems). b MUC20 knockdown inhibited phosphorylation of MET and AKT in HPAC and HPAF-II cells treated with 25 ng/ml HGF. c MUC20 knockdown inhibited HGF-triggered migration and invasion in HPAC and HPAF-II cells analysed by Transwell migration assay and Matrigel invasion assay, respectively. Scale bars indicate 1 mm. d MUC20 knockdown significantly reversed HGF-enhanced viability in HPAC and HPAF-II cells. Cells cultured in 10% FBS/DMEM-F12 were treated with PBS, MET inhibitor PHA665752 (2.5 µM) or HGF (25 ng/ml), as indicated. Viability was analysed by the MTT assay. Data are presented as mean ( n = 3) ± SD. * P

    Article Snippet: DMEM-F12 GlutaMAXTM (Invitrogen) was used for CFPAC-1, Capan-2, HPAC, HPAF-II, and PSC cells.

    Techniques: Cell Culture, Western Blot, Migration, Transwell Migration Assay, Invasion Assay, MTT Assay