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  • 90
    OriGene recombinant g6pd protein
    Aspirin-mediated acetylation of glucose-6-phosphate dehydrogenase <t>(G6PD)</t> is greater in (A) HCT 116 cells compared with in (B) HT-29 cells. Subconfluent cells were left untreated or were treated with aspirin for 24 h, lysates were prepared, and equal amounts of protein were immunoprecipitated with rabbit agarose-conjugated anti-acetyl lysine antibody. Agarose-bound proteins were eluted and immunoblotted with anti-G6PD antibody. (C) HCT-116 and (D) HT-29 samples were immunoblotted with anti-G6PD antibody. The experiments were repeated three times.
    Recombinant G6pd Protein, supplied by OriGene, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/recombinant g6pd protein/product/OriGene
    Average 90 stars, based on 1 article reviews
    Price from $9.99 to $1999.99
    recombinant g6pd protein - by Bioz Stars, 2024-06
    90/100 stars
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    94
    Cell Signaling Technology Inc g6pd
    Butyrate suppressed <t>G6PD</t> abundance and DNA synthesis of colon cancer cells. (A) The concentration of intracellular metabolites associated with glucose metabolism in HCT116 cells being incubated with 2 mM butyrate (labeled as “NaB”) or PBS vehicle (marked as “Con”) for 24 h were measured by liquid chromatograph-mass spectrometer/mass spectrometer (LC-MS/MS). (B) The variation of central metabolites in HCT116 cells being incubated with 2 mM butyrate or PBS vehicle for 24 h was annotated in the schematic diagram of glycolysis pathway. (C) After HCT116 cells were incubated with 2 mM butyrate for 24 h, the expression levels of genes that regulate glucose metabolism were analyzed by qPCR. ( D) The protein levels of G6PD in HCT116 and LoVo cells after being treated with 2 mM butyrate for 24 h were tested by western blotting. (E) After incubating HCT116 and LoVo cells with 2 mM butyrate or PBS vehicle for 24 h, DNA synthesis efficiency in both cell lines was measured by BrdU incorporation assay. The error bars showed standard deviations from independent triplicates. * p < 0.05, ** p < 0.01, *** p < 0.001. G6P, D-Glucose 6-phosphate; F6P, Beta-D-Fructose 6-phosphate; F1,6BP, D-Fructose 1,6-bisphosphate; PEP, Phosphoenolpyruvate; PYR, Pyruvate; LAC, Lactate; R5P, Ribose-5-phosphate.
    G6pd, supplied by Cell Signaling Technology Inc, used in various techniques. Bioz Stars score: 94/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/g6pd/product/Cell Signaling Technology Inc
    Average 94 stars, based on 1 article reviews
    Price from $9.99 to $1999.99
    g6pd - by Bioz Stars, 2024-06
    94/100 stars
      Buy from Supplier

    95
    Cell Signaling Technology Inc glucose 6 phosphate dehydrogenase
    Butyrate suppressed <t>G6PD</t> abundance and DNA synthesis of colon cancer cells. (A) The concentration of intracellular metabolites associated with glucose metabolism in HCT116 cells being incubated with 2 mM butyrate (labeled as “NaB”) or PBS vehicle (marked as “Con”) for 24 h were measured by liquid chromatograph-mass spectrometer/mass spectrometer (LC-MS/MS). (B) The variation of central metabolites in HCT116 cells being incubated with 2 mM butyrate or PBS vehicle for 24 h was annotated in the schematic diagram of glycolysis pathway. (C) After HCT116 cells were incubated with 2 mM butyrate for 24 h, the expression levels of genes that regulate glucose metabolism were analyzed by qPCR. ( D) The protein levels of G6PD in HCT116 and LoVo cells after being treated with 2 mM butyrate for 24 h were tested by western blotting. (E) After incubating HCT116 and LoVo cells with 2 mM butyrate or PBS vehicle for 24 h, DNA synthesis efficiency in both cell lines was measured by BrdU incorporation assay. The error bars showed standard deviations from independent triplicates. * p < 0.05, ** p < 0.01, *** p < 0.001. G6P, D-Glucose 6-phosphate; F6P, Beta-D-Fructose 6-phosphate; F1,6BP, D-Fructose 1,6-bisphosphate; PEP, Phosphoenolpyruvate; PYR, Pyruvate; LAC, Lactate; R5P, Ribose-5-phosphate.
    Glucose 6 Phosphate Dehydrogenase, supplied by Cell Signaling Technology Inc, used in various techniques. Bioz Stars score: 95/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/glucose 6 phosphate dehydrogenase/product/Cell Signaling Technology Inc
    Average 95 stars, based on 1 article reviews
    Price from $9.99 to $1999.99
    glucose 6 phosphate dehydrogenase - by Bioz Stars, 2024-06
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    94
    Valiant Co Ltd glucose-6-phosphate dehydrogenase
    Butyrate suppressed <t>G6PD</t> abundance and DNA synthesis of colon cancer cells. (A) The concentration of intracellular metabolites associated with glucose metabolism in HCT116 cells being incubated with 2 mM butyrate (labeled as “NaB”) or PBS vehicle (marked as “Con”) for 24 h were measured by liquid chromatograph-mass spectrometer/mass spectrometer (LC-MS/MS). (B) The variation of central metabolites in HCT116 cells being incubated with 2 mM butyrate or PBS vehicle for 24 h was annotated in the schematic diagram of glycolysis pathway. (C) After HCT116 cells were incubated with 2 mM butyrate for 24 h, the expression levels of genes that regulate glucose metabolism were analyzed by qPCR. ( D) The protein levels of G6PD in HCT116 and LoVo cells after being treated with 2 mM butyrate for 24 h were tested by western blotting. (E) After incubating HCT116 and LoVo cells with 2 mM butyrate or PBS vehicle for 24 h, DNA synthesis efficiency in both cell lines was measured by BrdU incorporation assay. The error bars showed standard deviations from independent triplicates. * p < 0.05, ** p < 0.01, *** p < 0.001. G6P, D-Glucose 6-phosphate; F6P, Beta-D-Fructose 6-phosphate; F1,6BP, D-Fructose 1,6-bisphosphate; PEP, Phosphoenolpyruvate; PYR, Pyruvate; LAC, Lactate; R5P, Ribose-5-phosphate.
    Glucose 6 Phosphate Dehydrogenase, supplied by Valiant Co Ltd, used in various techniques. Bioz Stars score: 94/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/glucose-6-phosphate dehydrogenase/product/Valiant Co Ltd
    Average 94 stars, based on 1 article reviews
    Price from $9.99 to $1999.99
    glucose-6-phosphate dehydrogenase - by Bioz Stars, 2024-06
    94/100 stars
      Buy from Supplier

    90
    OriGene glucose 6 phosphate dehydrogenase (g6pd) human qpcr primer pair
    Butyrate suppressed <t>G6PD</t> abundance and DNA synthesis of colon cancer cells. (A) The concentration of intracellular metabolites associated with glucose metabolism in HCT116 cells being incubated with 2 mM butyrate (labeled as “NaB”) or PBS vehicle (marked as “Con”) for 24 h were measured by liquid chromatograph-mass spectrometer/mass spectrometer (LC-MS/MS). (B) The variation of central metabolites in HCT116 cells being incubated with 2 mM butyrate or PBS vehicle for 24 h was annotated in the schematic diagram of glycolysis pathway. (C) After HCT116 cells were incubated with 2 mM butyrate for 24 h, the expression levels of genes that regulate glucose metabolism were analyzed by qPCR. ( D) The protein levels of G6PD in HCT116 and LoVo cells after being treated with 2 mM butyrate for 24 h were tested by western blotting. (E) After incubating HCT116 and LoVo cells with 2 mM butyrate or PBS vehicle for 24 h, DNA synthesis efficiency in both cell lines was measured by BrdU incorporation assay. The error bars showed standard deviations from independent triplicates. * p < 0.05, ** p < 0.01, *** p < 0.001. G6P, D-Glucose 6-phosphate; F6P, Beta-D-Fructose 6-phosphate; F1,6BP, D-Fructose 1,6-bisphosphate; PEP, Phosphoenolpyruvate; PYR, Pyruvate; LAC, Lactate; R5P, Ribose-5-phosphate.
    Glucose 6 Phosphate Dehydrogenase (G6pd) Human Qpcr Primer Pair, supplied by OriGene, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/glucose 6 phosphate dehydrogenase (g6pd) human qpcr primer pair/product/OriGene
    Average 90 stars, based on 1 article reviews
    Price from $9.99 to $1999.99
    glucose 6 phosphate dehydrogenase (g6pd) human qpcr primer pair - by Bioz Stars, 2024-06
    90/100 stars
      Buy from Supplier

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    Aspirin-mediated acetylation of glucose-6-phosphate dehydrogenase (G6PD) is greater in (A) HCT 116 cells compared with in (B) HT-29 cells. Subconfluent cells were left untreated or were treated with aspirin for 24 h, lysates were prepared, and equal amounts of protein were immunoprecipitated with rabbit agarose-conjugated anti-acetyl lysine antibody. Agarose-bound proteins were eluted and immunoblotted with anti-G6PD antibody. (C) HCT-116 and (D) HT-29 samples were immunoblotted with anti-G6PD antibody. The experiments were repeated three times.

    Journal: Molecular Medicine Reports

    Article Title: Aspirin inhibits glucose-6-phosphate dehydrogenase activity in HCT 116 cells through acetylation: Identification of aspirin-acetylated sites

    doi: 10.3892/mmr.2016.5449

    Figure Lengend Snippet: Aspirin-mediated acetylation of glucose-6-phosphate dehydrogenase (G6PD) is greater in (A) HCT 116 cells compared with in (B) HT-29 cells. Subconfluent cells were left untreated or were treated with aspirin for 24 h, lysates were prepared, and equal amounts of protein were immunoprecipitated with rabbit agarose-conjugated anti-acetyl lysine antibody. Agarose-bound proteins were eluted and immunoblotted with anti-G6PD antibody. (C) HCT-116 and (D) HT-29 samples were immunoblotted with anti-G6PD antibody. The experiments were repeated three times.

    Article Snippet: The recombinant G6PD protein (isoform a ) was obtained from Origene Technologies, Inc. (Rockville, MD, USA).

    Techniques: Immunoprecipitation

    Effects of aspirin on glucose-6-phosphate dehydrogenase (G6PD) activity in HCT 116 and HT-29 cells. Cells were cultured and left untreated or were treated with aspirin for 24 h. Protein (100 µ g) was used to conduct a G6PD assay. The reaction mixture was incubated at 37°C for 30 min, and absorbance was measured at 450 nm. G6PD activity was expressed as a percentage of control. The experiments were repeated three times. Data are represented as mean ± standard deviation. * P<0.05, ** P<0.01, *** P<0.001 vs. the control.

    Journal: Molecular Medicine Reports

    Article Title: Aspirin inhibits glucose-6-phosphate dehydrogenase activity in HCT 116 cells through acetylation: Identification of aspirin-acetylated sites

    doi: 10.3892/mmr.2016.5449

    Figure Lengend Snippet: Effects of aspirin on glucose-6-phosphate dehydrogenase (G6PD) activity in HCT 116 and HT-29 cells. Cells were cultured and left untreated or were treated with aspirin for 24 h. Protein (100 µ g) was used to conduct a G6PD assay. The reaction mixture was incubated at 37°C for 30 min, and absorbance was measured at 450 nm. G6PD activity was expressed as a percentage of control. The experiments were repeated three times. Data are represented as mean ± standard deviation. * P<0.05, ** P<0.01, *** P<0.001 vs. the control.

    Article Snippet: The recombinant G6PD protein (isoform a ) was obtained from Origene Technologies, Inc. (Rockville, MD, USA).

    Techniques: Activity Assay, Cell Culture, G6PD Assay, Incubation, Standard Deviation

    Mass spectrometry (MS) analysis of recombinant glucose-6-phosphate dehydrogenase (G6PD) isoform a . In vitro acetylation of recombinant G6PD by aspirin. (A) A total of 5 ng in vitro acetylated recombinant G6PD was immunoblotted with anti-acetyl lysine antibody and the protein band was detected by enhanced chemiluminescence. (B-D) MS/MS fragmentation spectra showing acetyl modification of (B) K77, (C) K201 and (D) K235.

    Journal: Molecular Medicine Reports

    Article Title: Aspirin inhibits glucose-6-phosphate dehydrogenase activity in HCT 116 cells through acetylation: Identification of aspirin-acetylated sites

    doi: 10.3892/mmr.2016.5449

    Figure Lengend Snippet: Mass spectrometry (MS) analysis of recombinant glucose-6-phosphate dehydrogenase (G6PD) isoform a . In vitro acetylation of recombinant G6PD by aspirin. (A) A total of 5 ng in vitro acetylated recombinant G6PD was immunoblotted with anti-acetyl lysine antibody and the protein band was detected by enhanced chemiluminescence. (B-D) MS/MS fragmentation spectra showing acetyl modification of (B) K77, (C) K201 and (D) K235.

    Article Snippet: The recombinant G6PD protein (isoform a ) was obtained from Origene Technologies, Inc. (Rockville, MD, USA).

    Techniques: Mass Spectrometry, Recombinant, In Vitro, Tandem Mass Spectroscopy, Modification

    Location of aspirin-acetylated lysine residues identified in the present study and the naturally acetylated lysines on  G6PD  ( <xref ref-type= 25 )." width="100%" height="100%">

    Journal: Molecular Medicine Reports

    Article Title: Aspirin inhibits glucose-6-phosphate dehydrogenase activity in HCT 116 cells through acetylation: Identification of aspirin-acetylated sites

    doi: 10.3892/mmr.2016.5449

    Figure Lengend Snippet: Location of aspirin-acetylated lysine residues identified in the present study and the naturally acetylated lysines on G6PD ( 25 ).

    Article Snippet: The recombinant G6PD protein (isoform a ) was obtained from Origene Technologies, Inc. (Rockville, MD, USA).

    Techniques:

    3-Dimension space-filling model of recombinant glucose-6-phosphate dehydrogenase (G6PD; NP_000393), is shown. The location of aspirin-acetylated lysine residues are highlighted in blue (K77, K112, K119, K201, K235, K390, K396, K416, K438, K459, K462, K527, K538, K544).

    Journal: Molecular Medicine Reports

    Article Title: Aspirin inhibits glucose-6-phosphate dehydrogenase activity in HCT 116 cells through acetylation: Identification of aspirin-acetylated sites

    doi: 10.3892/mmr.2016.5449

    Figure Lengend Snippet: 3-Dimension space-filling model of recombinant glucose-6-phosphate dehydrogenase (G6PD; NP_000393), is shown. The location of aspirin-acetylated lysine residues are highlighted in blue (K77, K112, K119, K201, K235, K390, K396, K416, K438, K459, K462, K527, K538, K544).

    Article Snippet: The recombinant G6PD protein (isoform a ) was obtained from Origene Technologies, Inc. (Rockville, MD, USA).

    Techniques: Recombinant

    Butyrate suppressed G6PD abundance and DNA synthesis of colon cancer cells. (A) The concentration of intracellular metabolites associated with glucose metabolism in HCT116 cells being incubated with 2 mM butyrate (labeled as “NaB”) or PBS vehicle (marked as “Con”) for 24 h were measured by liquid chromatograph-mass spectrometer/mass spectrometer (LC-MS/MS). (B) The variation of central metabolites in HCT116 cells being incubated with 2 mM butyrate or PBS vehicle for 24 h was annotated in the schematic diagram of glycolysis pathway. (C) After HCT116 cells were incubated with 2 mM butyrate for 24 h, the expression levels of genes that regulate glucose metabolism were analyzed by qPCR. ( D) The protein levels of G6PD in HCT116 and LoVo cells after being treated with 2 mM butyrate for 24 h were tested by western blotting. (E) After incubating HCT116 and LoVo cells with 2 mM butyrate or PBS vehicle for 24 h, DNA synthesis efficiency in both cell lines was measured by BrdU incorporation assay. The error bars showed standard deviations from independent triplicates. * p < 0.05, ** p < 0.01, *** p < 0.001. G6P, D-Glucose 6-phosphate; F6P, Beta-D-Fructose 6-phosphate; F1,6BP, D-Fructose 1,6-bisphosphate; PEP, Phosphoenolpyruvate; PYR, Pyruvate; LAC, Lactate; R5P, Ribose-5-phosphate.

    Journal: Frontiers in Molecular Biosciences

    Article Title: Butyrate Suppresses Glucose Metabolism of Colorectal Cancer Cells via GPR109a-AKT Signaling Pathway and Enhances Chemotherapy

    doi: 10.3389/fmolb.2021.634874

    Figure Lengend Snippet: Butyrate suppressed G6PD abundance and DNA synthesis of colon cancer cells. (A) The concentration of intracellular metabolites associated with glucose metabolism in HCT116 cells being incubated with 2 mM butyrate (labeled as “NaB”) or PBS vehicle (marked as “Con”) for 24 h were measured by liquid chromatograph-mass spectrometer/mass spectrometer (LC-MS/MS). (B) The variation of central metabolites in HCT116 cells being incubated with 2 mM butyrate or PBS vehicle for 24 h was annotated in the schematic diagram of glycolysis pathway. (C) After HCT116 cells were incubated with 2 mM butyrate for 24 h, the expression levels of genes that regulate glucose metabolism were analyzed by qPCR. ( D) The protein levels of G6PD in HCT116 and LoVo cells after being treated with 2 mM butyrate for 24 h were tested by western blotting. (E) After incubating HCT116 and LoVo cells with 2 mM butyrate or PBS vehicle for 24 h, DNA synthesis efficiency in both cell lines was measured by BrdU incorporation assay. The error bars showed standard deviations from independent triplicates. * p < 0.05, ** p < 0.01, *** p < 0.001. G6P, D-Glucose 6-phosphate; F6P, Beta-D-Fructose 6-phosphate; F1,6BP, D-Fructose 1,6-bisphosphate; PEP, Phosphoenolpyruvate; PYR, Pyruvate; LAC, Lactate; R5P, Ribose-5-phosphate.

    Article Snippet: Antibodies against GLUT1 (1:1000) and G6PD (1:1000) were purchased from Cell Signaling Technology (United States).

    Techniques: DNA Synthesis, Concentration Assay, Incubation, Labeling, Mass Spectrometry, Liquid Chromatography with Mass Spectroscopy, Expressing, Western Blot, BrdU Incorporation Assay

    Butyrate regulated G6PD abundance and DNA synthesis of colon cancer cells through the AKT pathway. (A ) The protein levels of G6PD were measured by western blotting in HCT116 and LoVo cells being treated with 2 mM butyrate alone or co-incubated with 2 mM butyrate and 10.96 μM SC79 for 24 h. (B) The effects of 10.96 μM SC79 (marked as “NaB + SC79”) or PBS vehicle (labeled as “NaB + Con”) on cell proliferation were tested by CCK-8 assay in HCT116 and LoVo cells which were incubated with 2 mM butyrate for 24 h. (C) After being treated with a combination of 2 mM butyrate and PBS vehicle (labeled as “NaB + Con”) or 2 mM butyrate and 10.96 μM SC79 (marked as “NaB + SC79”) for 24 h, the DNA synthesis efficiency in HCT116 and LoVo cells was assayed by BrdU incorporation experiment. The error bars showed standard deviations from independent triplicates. * p < 0.05, *** p < 0.001.

    Journal: Frontiers in Molecular Biosciences

    Article Title: Butyrate Suppresses Glucose Metabolism of Colorectal Cancer Cells via GPR109a-AKT Signaling Pathway and Enhances Chemotherapy

    doi: 10.3389/fmolb.2021.634874

    Figure Lengend Snippet: Butyrate regulated G6PD abundance and DNA synthesis of colon cancer cells through the AKT pathway. (A ) The protein levels of G6PD were measured by western blotting in HCT116 and LoVo cells being treated with 2 mM butyrate alone or co-incubated with 2 mM butyrate and 10.96 μM SC79 for 24 h. (B) The effects of 10.96 μM SC79 (marked as “NaB + SC79”) or PBS vehicle (labeled as “NaB + Con”) on cell proliferation were tested by CCK-8 assay in HCT116 and LoVo cells which were incubated with 2 mM butyrate for 24 h. (C) After being treated with a combination of 2 mM butyrate and PBS vehicle (labeled as “NaB + Con”) or 2 mM butyrate and 10.96 μM SC79 (marked as “NaB + SC79”) for 24 h, the DNA synthesis efficiency in HCT116 and LoVo cells was assayed by BrdU incorporation experiment. The error bars showed standard deviations from independent triplicates. * p < 0.05, *** p < 0.001.

    Article Snippet: Antibodies against GLUT1 (1:1000) and G6PD (1:1000) were purchased from Cell Signaling Technology (United States).

    Techniques: DNA Synthesis, Western Blot, Incubation, Labeling, CCK-8 Assay, BrdU Incorporation Assay