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  • 98
    Addgene inc pcscmv tdtomato
    Pcscmv Tdtomato, supplied by Addgene inc, used in various techniques. Bioz Stars score: 98/100, based on 48 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/pcscmv tdtomato/product/Addgene inc
    Average 98 stars, based on 48 article reviews
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    pcscmv tdtomato - by Bioz Stars, 2020-02
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    81
    Addgene inc tdp 43 5fl
    RNA binding ability of <t>TDP-43</t> is important for regulating dendritic branching ( a ) (top) Schematic of TDP-43 protein demonstrating the C-terminal fragment (CTF, amino acid residues 170–414) being overexpressed. RRM2: RNA Recognition Motif, NES: Nuclear Export Signal, GRD: Glycine Rich Domain. (bottom) Sholl analysis for indicated conditions (N = 60 for all conditions). The colour of the *represents the experimental condition being tested against control. ( b ) (top) Schematic of TDP-43 protein demonstrating TDP-43 ∆N (amino acid residues 10–414). NLS: Nuclear Localization Signal, RRM1: RNA Recognition Motif. (bottom) Sholl analysis for indicated conditions (Control, N = 57, TDP-43 overexpression, N = 45, TDP-43 ∆N overexpression, N = 54). ( c ) (top) Schematic of TDP-43 protein demonstrating TDP-43 <t>5FL</t> (F147L/F149L/F194L/F229L/F231L). (bottom) Sholl analysis for indicated conditions (Control, N = 87, TDP-43 overexpression, N = 76, TDP-43 5FL overexpression, N = 90). The colour of the *represent the experimental condition being tested against control. ( a–c ) Grey vs pink = not significant. Two-Way ANOVA with Tukey’s test, *p
    Tdp 43 5fl, supplied by Addgene inc, used in various techniques. Bioz Stars score: 81/100, based on 6 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    84
    Addgene inc rfp lamp1
    RNA binding ability of <t>TDP-43</t> is important for regulating dendritic branching ( a ) (top) Schematic of TDP-43 protein demonstrating the C-terminal fragment (CTF, amino acid residues 170–414) being overexpressed. RRM2: RNA Recognition Motif, NES: Nuclear Export Signal, GRD: Glycine Rich Domain. (bottom) Sholl analysis for indicated conditions (N = 60 for all conditions). The colour of the *represents the experimental condition being tested against control. ( b ) (top) Schematic of TDP-43 protein demonstrating TDP-43 ∆N (amino acid residues 10–414). NLS: Nuclear Localization Signal, RRM1: RNA Recognition Motif. (bottom) Sholl analysis for indicated conditions (Control, N = 57, TDP-43 overexpression, N = 45, TDP-43 ∆N overexpression, N = 54). ( c ) (top) Schematic of TDP-43 protein demonstrating TDP-43 <t>5FL</t> (F147L/F149L/F194L/F229L/F231L). (bottom) Sholl analysis for indicated conditions (Control, N = 87, TDP-43 overexpression, N = 76, TDP-43 5FL overexpression, N = 90). The colour of the *represent the experimental condition being tested against control. ( a–c ) Grey vs pink = not significant. Two-Way ANOVA with Tukey’s test, *p
    Rfp Lamp1, supplied by Addgene inc, used in various techniques. Bioz Stars score: 84/100, based on 30 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/rfp lamp1/product/Addgene inc
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    80
    Addgene inc full length human fus
    RNA binding ability of <t>TDP-43</t> is important for regulating dendritic branching ( a ) (top) Schematic of TDP-43 protein demonstrating the C-terminal fragment (CTF, amino acid residues 170–414) being overexpressed. RRM2: RNA Recognition Motif, NES: Nuclear Export Signal, GRD: Glycine Rich Domain. (bottom) Sholl analysis for indicated conditions (N = 60 for all conditions). The colour of the *represents the experimental condition being tested against control. ( b ) (top) Schematic of TDP-43 protein demonstrating TDP-43 ∆N (amino acid residues 10–414). NLS: Nuclear Localization Signal, RRM1: RNA Recognition Motif. (bottom) Sholl analysis for indicated conditions (Control, N = 57, TDP-43 overexpression, N = 45, TDP-43 ∆N overexpression, N = 54). ( c ) (top) Schematic of TDP-43 protein demonstrating TDP-43 <t>5FL</t> (F147L/F149L/F194L/F229L/F231L). (bottom) Sholl analysis for indicated conditions (Control, N = 87, TDP-43 overexpression, N = 76, TDP-43 5FL overexpression, N = 90). The colour of the *represent the experimental condition being tested against control. ( a–c ) Grey vs pink = not significant. Two-Way ANOVA with Tukey’s test, *p
    Full Length Human Fus, supplied by Addgene inc, used in various techniques. Bioz Stars score: 80/100, based on 6 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    94
    Addgene inc α synuclein
    FUS and <t>α</t> -synuclein proteinopathies are associated with changes in histone H2B phosphorylation levels. Shown are representative immunoblots displaying the levels of H2BT129ph for (a) TDP-43 and FUS and (b) α -synuclein yeast proteinopathy models. Quantitation histograms compiling multiple biological replicates are presented alongside the blots. All of the graphs display the mean fold change in modification levels for each group based on densitometric analysis of Western blots. Error bars indicate +SEM ( n ≥ 3) for each modification. *, p
    α Synuclein, supplied by Addgene inc, used in various techniques. Bioz Stars score: 94/100, based on 24 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    Image Search Results


    RNA binding ability of TDP-43 is important for regulating dendritic branching ( a ) (top) Schematic of TDP-43 protein demonstrating the C-terminal fragment (CTF, amino acid residues 170–414) being overexpressed. RRM2: RNA Recognition Motif, NES: Nuclear Export Signal, GRD: Glycine Rich Domain. (bottom) Sholl analysis for indicated conditions (N = 60 for all conditions). The colour of the *represents the experimental condition being tested against control. ( b ) (top) Schematic of TDP-43 protein demonstrating TDP-43 ∆N (amino acid residues 10–414). NLS: Nuclear Localization Signal, RRM1: RNA Recognition Motif. (bottom) Sholl analysis for indicated conditions (Control, N = 57, TDP-43 overexpression, N = 45, TDP-43 ∆N overexpression, N = 54). ( c ) (top) Schematic of TDP-43 protein demonstrating TDP-43 5FL (F147L/F149L/F194L/F229L/F231L). (bottom) Sholl analysis for indicated conditions (Control, N = 87, TDP-43 overexpression, N = 76, TDP-43 5FL overexpression, N = 90). The colour of the *represent the experimental condition being tested against control. ( a–c ) Grey vs pink = not significant. Two-Way ANOVA with Tukey’s test, *p

    Journal: Scientific Reports

    Article Title: TDP-43 misexpression causes defects in dendritic growth

    doi: 10.1038/s41598-017-15914-4

    Figure Lengend Snippet: RNA binding ability of TDP-43 is important for regulating dendritic branching ( a ) (top) Schematic of TDP-43 protein demonstrating the C-terminal fragment (CTF, amino acid residues 170–414) being overexpressed. RRM2: RNA Recognition Motif, NES: Nuclear Export Signal, GRD: Glycine Rich Domain. (bottom) Sholl analysis for indicated conditions (N = 60 for all conditions). The colour of the *represents the experimental condition being tested against control. ( b ) (top) Schematic of TDP-43 protein demonstrating TDP-43 ∆N (amino acid residues 10–414). NLS: Nuclear Localization Signal, RRM1: RNA Recognition Motif. (bottom) Sholl analysis for indicated conditions (Control, N = 57, TDP-43 overexpression, N = 45, TDP-43 ∆N overexpression, N = 54). ( c ) (top) Schematic of TDP-43 protein demonstrating TDP-43 5FL (F147L/F149L/F194L/F229L/F231L). (bottom) Sholl analysis for indicated conditions (Control, N = 87, TDP-43 overexpression, N = 76, TDP-43 5FL overexpression, N = 90). The colour of the *represent the experimental condition being tested against control. ( a–c ) Grey vs pink = not significant. Two-Way ANOVA with Tukey’s test, *p

    Article Snippet: Plasmids expressing full-length human FUS (Addgene plasmid # 29609) and TDP-43 5FL (Addgene plasmid # 84914) was a gift from Aaron Gitler (Stanford University School of Medicine, Stanford CA). pCSCMV:tdTomato was a gift from Gerhart Ryffel (Addgene plasmid # 30530).

    Techniques: RNA Binding Assay, Over Expression

    FUS and α -synuclein proteinopathies are associated with changes in histone H2B phosphorylation levels. Shown are representative immunoblots displaying the levels of H2BT129ph for (a) TDP-43 and FUS and (b) α -synuclein yeast proteinopathy models. Quantitation histograms compiling multiple biological replicates are presented alongside the blots. All of the graphs display the mean fold change in modification levels for each group based on densitometric analysis of Western blots. Error bars indicate +SEM ( n ≥ 3) for each modification. *, p

    Journal: ACS chemical neuroscience

    Article Title: Neurodegenerative Disease Proteinopathies Are Connected to Distinct Histone Post-translational Modification Landscapes

    doi: 10.1021/acschemneuro.7b00297

    Figure Lengend Snippet: FUS and α -synuclein proteinopathies are associated with changes in histone H2B phosphorylation levels. Shown are representative immunoblots displaying the levels of H2BT129ph for (a) TDP-43 and FUS and (b) α -synuclein yeast proteinopathy models. Quantitation histograms compiling multiple biological replicates are presented alongside the blots. All of the graphs display the mean fold change in modification levels for each group based on densitometric analysis of Western blots. Error bars indicate +SEM ( n ≥ 3) for each modification. *, p

    Article Snippet: Vectors encoding TDP-43, FUS, and α -synuclein (pAG303GAL-TDP-43, pAG303GAL-FUS, and pAG303GAL- α -synu-clein-GFP, respectively) were a gift from A. Gitler., , A pAG303GAL-ccdB vector, which was used as a control, was a gift from Susan Lindquist (Addgene plasmid no. 14133).

    Techniques: Western Blot, Quantitation Assay, Modification

    α -Synuclein and TDP-43 proteinopathy models display decreases in histone H3 di- and trimethylation, respectively, on lysine 36. Shown are representative immunoblots displaying the levels of (a, c) H3K36me2 and (b, d) H3K36me3 for TDP-43 and FUS and for α -synuclein yeast proteinopathy models, respectively. Quantitation histograms compiling multiple biological replicates are presented alongside the blots. All of the graphs display the mean fold change in modification levels for each group based on densitometric analysis of Western blots. Error bars indicate the +SEM ( n = 3–7) for each modification. **, p

    Journal: ACS chemical neuroscience

    Article Title: Neurodegenerative Disease Proteinopathies Are Connected to Distinct Histone Post-translational Modification Landscapes

    doi: 10.1021/acschemneuro.7b00297

    Figure Lengend Snippet: α -Synuclein and TDP-43 proteinopathy models display decreases in histone H3 di- and trimethylation, respectively, on lysine 36. Shown are representative immunoblots displaying the levels of (a, c) H3K36me2 and (b, d) H3K36me3 for TDP-43 and FUS and for α -synuclein yeast proteinopathy models, respectively. Quantitation histograms compiling multiple biological replicates are presented alongside the blots. All of the graphs display the mean fold change in modification levels for each group based on densitometric analysis of Western blots. Error bars indicate the +SEM ( n = 3–7) for each modification. **, p

    Article Snippet: Vectors encoding TDP-43, FUS, and α -synuclein (pAG303GAL-TDP-43, pAG303GAL-FUS, and pAG303GAL- α -synu-clein-GFP, respectively) were a gift from A. Gitler., , A pAG303GAL-ccdB vector, which was used as a control, was a gift from Susan Lindquist (Addgene plasmid no. 14133).

    Techniques: Western Blot, Quantitation Assay, Modification

    FUS overexpression correlates with changes in phosphorylation on serine 10 and acetylation on lysine 14 and lysine 56 of histone H3. Shown are representative immunoblots displaying the levels of (a, d) H3S10ph, (b, e) H3K14ac, and (c, f) H3K56ac for TDP-43 and FUS and for α -synuclein yeast proteinopathy models, respectively. Quantitation histograms compiling multiple biological replicates are presented alongside the blots. All of the graphs display the mean fold change in modification levels for each group based on densitometric analysis of Western blots. Error bars indicate +SEM ( n = 3–6) for each modification. **, p

    Journal: ACS chemical neuroscience

    Article Title: Neurodegenerative Disease Proteinopathies Are Connected to Distinct Histone Post-translational Modification Landscapes

    doi: 10.1021/acschemneuro.7b00297

    Figure Lengend Snippet: FUS overexpression correlates with changes in phosphorylation on serine 10 and acetylation on lysine 14 and lysine 56 of histone H3. Shown are representative immunoblots displaying the levels of (a, d) H3S10ph, (b, e) H3K14ac, and (c, f) H3K56ac for TDP-43 and FUS and for α -synuclein yeast proteinopathy models, respectively. Quantitation histograms compiling multiple biological replicates are presented alongside the blots. All of the graphs display the mean fold change in modification levels for each group based on densitometric analysis of Western blots. Error bars indicate +SEM ( n = 3–6) for each modification. **, p

    Article Snippet: Vectors encoding TDP-43, FUS, and α -synuclein (pAG303GAL-TDP-43, pAG303GAL-FUS, and pAG303GAL- α -synu-clein-GFP, respectively) were a gift from A. Gitler., , A pAG303GAL-ccdB vector, which was used as a control, was a gift from Susan Lindquist (Addgene plasmid no. 14133).

    Techniques: Over Expression, Western Blot, Quantitation Assay, Modification

    FUS and TDP-43 overexpression correlate with changes in arginine dimethylation and lysine acetylation of histone H4. Shown are representative immunoblots displaying the levels of (a, d) H4R3me2, (b, e) H4K12ac, and (c, f) H4K16ac for TDP-43 and FUS and for α -synuclein yeast proteinopathy models, respectively. Quantitation histograms compiling multiple biological replicates are presented alongside the blots. All of the graphs display the mean fold change in modification levels for each group based on densitometric analysis of Western blots. Error bars indicate +SEM ( n = 3–6) for each modification. *, p

    Journal: ACS chemical neuroscience

    Article Title: Neurodegenerative Disease Proteinopathies Are Connected to Distinct Histone Post-translational Modification Landscapes

    doi: 10.1021/acschemneuro.7b00297

    Figure Lengend Snippet: FUS and TDP-43 overexpression correlate with changes in arginine dimethylation and lysine acetylation of histone H4. Shown are representative immunoblots displaying the levels of (a, d) H4R3me2, (b, e) H4K12ac, and (c, f) H4K16ac for TDP-43 and FUS and for α -synuclein yeast proteinopathy models, respectively. Quantitation histograms compiling multiple biological replicates are presented alongside the blots. All of the graphs display the mean fold change in modification levels for each group based on densitometric analysis of Western blots. Error bars indicate +SEM ( n = 3–6) for each modification. *, p

    Article Snippet: Vectors encoding TDP-43, FUS, and α -synuclein (pAG303GAL-TDP-43, pAG303GAL-FUS, and pAG303GAL- α -synu-clein-GFP, respectively) were a gift from A. Gitler., , A pAG303GAL-ccdB vector, which was used as a control, was a gift from Susan Lindquist (Addgene plasmid no. 14133).

    Techniques: Over Expression, Western Blot, Quantitation Assay, Modification

    RNA levels are decreased in a FUS overexpression ALS model. (a) Total RNA levels in yeast control cells and cells overexpressing TDP-43 and FUS. (b) Total RNA levels in yeast control cells and cells overexpressing  α -synuclein. All of the graphs display the mean fold change in total RNA levels for each group. Error bars indicate +SEM ( n  = 3) for each group. *,  p

    Journal: ACS chemical neuroscience

    Article Title: Neurodegenerative Disease Proteinopathies Are Connected to Distinct Histone Post-translational Modification Landscapes

    doi: 10.1021/acschemneuro.7b00297

    Figure Lengend Snippet: RNA levels are decreased in a FUS overexpression ALS model. (a) Total RNA levels in yeast control cells and cells overexpressing TDP-43 and FUS. (b) Total RNA levels in yeast control cells and cells overexpressing α -synuclein. All of the graphs display the mean fold change in total RNA levels for each group. Error bars indicate +SEM ( n = 3) for each group. *, p

    Article Snippet: Vectors encoding TDP-43, FUS, and α -synuclein (pAG303GAL-TDP-43, pAG303GAL-FUS, and pAG303GAL- α -synu-clein-GFP, respectively) were a gift from A. Gitler., , A pAG303GAL-ccdB vector, which was used as a control, was a gift from Susan Lindquist (Addgene plasmid no. 14133).

    Techniques: Over Expression

    Histone modifications are altered in the context of yeast overexpressing TDP-43, FUS, and α -synuclein ( α -Syn). Shown are p values for the ratios of histone H2A, H2B, H3, and H4 PTM abundances in yeast strains expressing TDP-43, FUS, and α -Syn relative to control cells. The scale is based on p values derived from statistical analysis of Western blotting experiments. The p values were calculated using a two-tailed t test with Welch’s modification. Green indicates more statistically significant decreased modification levels, while red indicates more statistically significant increased modification levels.

    Journal: ACS chemical neuroscience

    Article Title: Neurodegenerative Disease Proteinopathies Are Connected to Distinct Histone Post-translational Modification Landscapes

    doi: 10.1021/acschemneuro.7b00297

    Figure Lengend Snippet: Histone modifications are altered in the context of yeast overexpressing TDP-43, FUS, and α -synuclein ( α -Syn). Shown are p values for the ratios of histone H2A, H2B, H3, and H4 PTM abundances in yeast strains expressing TDP-43, FUS, and α -Syn relative to control cells. The scale is based on p values derived from statistical analysis of Western blotting experiments. The p values were calculated using a two-tailed t test with Welch’s modification. Green indicates more statistically significant decreased modification levels, while red indicates more statistically significant increased modification levels.

    Article Snippet: Vectors encoding TDP-43, FUS, and α -synuclein (pAG303GAL-TDP-43, pAG303GAL-FUS, and pAG303GAL- α -synu-clein-GFP, respectively) were a gift from A. Gitler., , A pAG303GAL-ccdB vector, which was used as a control, was a gift from Susan Lindquist (Addgene plasmid no. 14133).

    Techniques: Expressing, Derivative Assay, Western Blot, Two Tailed Test, Modification