Journal: ACS chemical neuroscience
Article Title: Neurodegenerative Disease Proteinopathies Are Connected to Distinct Histone Post-translational Modification Landscapes
Figure Lengend Snippet: Histone modifications are altered in the context of yeast overexpressing TDP-43, FUS, and α -synuclein ( α -Syn). Shown are p values for the ratios of histone H2A, H2B, H3, and H4 PTM abundances in yeast strains expressing TDP-43, FUS, and α -Syn relative to control cells. The scale is based on p values derived from statistical analysis of Western blotting experiments. The p values were calculated using a two-tailed t test with Welch’s modification. Green indicates more statistically significant decreased modification levels, while red indicates more statistically significant increased modification levels.
Article Snippet: Vectors encoding TDP-43, FUS, and α -synuclein (pAG303GAL-TDP-43, pAG303GAL-FUS, and pAG303GAL- α -synu-clein-GFP, respectively) were a gift from A. Gitler., , A pAG303GAL-ccdB vector, which was used as a control, was a gift from Susan Lindquist (Addgene plasmid no. 14133).
Techniques: Expressing, Derivative Assay, Western Blot, Two Tailed Test, Modification