gfp Affibody Search Results


  • Logo
  • About
  • News
  • Press Release
  • Team
  • Advisors
  • Partners
  • Contact
  • Bioz Stars
  • Bioz vStars
  • 95
    Millipore bl21
    Bl21, supplied by Millipore, used in various techniques. Bioz Stars score: 95/100, based on 6857 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/bl21/product/Millipore
    Average 95 stars, based on 6857 article reviews
    Price from $9.99 to $1999.99
    bl21 - by Bioz Stars, 2020-02
    95/100 stars
      Buy from Supplier

    79
    Affibody egfr gfp
    Egfr Gfp, supplied by Affibody, used in various techniques. Bioz Stars score: 79/100, based on 2 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/egfr gfp/product/Affibody
    Average 79 stars, based on 2 article reviews
    Price from $9.99 to $1999.99
    egfr gfp - by Bioz Stars, 2020-02
    79/100 stars
      Buy from Supplier

    93
    Affibody affibody peptide
    Affibody Peptide, supplied by Affibody, used in various techniques. Bioz Stars score: 93/100, based on 68 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/affibody peptide/product/Affibody
    Average 93 stars, based on 68 article reviews
    Price from $9.99 to $1999.99
    affibody peptide - by Bioz Stars, 2020-02
    93/100 stars
      Buy from Supplier

    77
    Affibody anti egfr affibody conjugated to alexa fluor 488
    Effect of logD and charge on <t>affibody</t> conjugate mobility. Plots of mean instantaneous D fit for different <t>anti-EGFR</t> Affibody conjugates vs charge at pH 7.4 ( A ), and logD ( B ). C) Plot of spot density for selected anti-EGFR Affibody conjugates vs charge at logD. Each datapoint corresponds to mean ± SEM of at least 10 independent areas. Lines show linear regression fit to the data, R 2 values indicating goodness of fit. <t>Alexa</t> 555 is not included in this figure as the structure is not published and charge and logD values are unavailable.
    Anti Egfr Affibody Conjugated To Alexa Fluor 488, supplied by Affibody, used in various techniques. Bioz Stars score: 77/100, based on 5 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/anti egfr affibody conjugated to alexa fluor 488/product/Affibody
    Average 77 stars, based on 5 article reviews
    Price from $9.99 to $1999.99
    anti egfr affibody conjugated to alexa fluor 488 - by Bioz Stars, 2020-02
    77/100 stars
      Buy from Supplier

    80
    Affibody anti egfr targeted affibody
    Effect of logD and charge on <t>affibody</t> conjugate mobility. Plots of mean instantaneous D fit for different <t>anti-EGFR</t> Affibody conjugates vs charge at pH 7.4 ( A ), and logD ( B ). C) Plot of spot density for selected anti-EGFR Affibody conjugates vs charge at logD. Each datapoint corresponds to mean ± SEM of at least 10 independent areas. Lines show linear regression fit to the data, R 2 values indicating goodness of fit. <t>Alexa</t> 555 is not included in this figure as the structure is not published and charge and logD values are unavailable.
    Anti Egfr Targeted Affibody, supplied by Affibody, used in various techniques. Bioz Stars score: 80/100, based on 10 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/anti egfr targeted affibody/product/Affibody
    Average 80 stars, based on 10 article reviews
    Price from $9.99 to $1999.99
    anti egfr targeted affibody - by Bioz Stars, 2020-02
    80/100 stars
      Buy from Supplier

    85
    Affibody sel tagged affibody molecules
    PET images, summed 30–60 min, and TACs from a Balbc nu/nu mouse (prone) bearing tumors ( white arrows ): a one A431 xenograft (1 × 10 7 cells, 15 days) or b two A431 xenografts ( left : 1 × 10 7 cells, 28 days; right : 1 × 10 7 cells, 25 days). Comparison A shows a 7-times higher uptake with targeting [methyl- 11 C]-Z EGFR:2377 -ST-CH 3 compared to the non-targeting [methyl- 11 C]-Z Taq:3638 -ST-CH 3 . Comparison B illustrates uptake of the targeting <t>Affibody</t> increasing as the tumors grow from time from inoculation
    Sel Tagged Affibody Molecules, supplied by Affibody, used in various techniques. Bioz Stars score: 85/100, based on 20 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/sel tagged affibody molecules/product/Affibody
    Average 85 stars, based on 20 article reviews
    Price from $9.99 to $1999.99
    sel tagged affibody molecules - by Bioz Stars, 2020-02
    85/100 stars
      Buy from Supplier

    90
    Abcam goat polyclonal antibody
    Plant-produced τGFP particles bind GFP. a) Ultracentrifuge tubes containing sucrose cushions photographed under UV light after ultracentrifugation. GFP-associated fluorescence remains in the supernatant when GFP-containing plant lysate is centrifuged alone or mixed with tEL-containing plant lysate; but migrates through the cushion when GFP-containing and τGFP-containing plant lysates are mixed. b) Detection of GFP by sandwich ELISA, after coating wells with τGFP (green), τglyc (orange) or an anti-GFP <t>polyclonal</t> IgG (blue) and adding GFP to the wells at four different concentrations after blocking. Detection is horseradish peroxidase—mediated ECL, and signal is net of background. Error bars are standard error. c) Electron micrograph of plant-produced τGFP particles in the presence of GFP, purified by sucrose cushion and size exclusion chromatography. Scale bar 100 nm.
    Goat Polyclonal Antibody, supplied by Abcam, used in various techniques. Bioz Stars score: 90/100, based on 739 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/goat polyclonal antibody/product/Abcam
    Average 90 stars, based on 739 article reviews
    Price from $9.99 to $1999.99
    goat polyclonal antibody - by Bioz Stars, 2020-02
    90/100 stars
      Buy from Supplier

    78
    Affibody raw fluorescence
    Plant-produced τGFP particles bind GFP. a) Ultracentrifuge tubes containing sucrose cushions photographed under UV light after ultracentrifugation. GFP-associated fluorescence remains in the supernatant when GFP-containing plant lysate is centrifuged alone or mixed with tEL-containing plant lysate; but migrates through the cushion when GFP-containing and τGFP-containing plant lysates are mixed. b) Detection of GFP by sandwich ELISA, after coating wells with τGFP (green), τglyc (orange) or an anti-GFP <t>polyclonal</t> IgG (blue) and adding GFP to the wells at four different concentrations after blocking. Detection is horseradish peroxidase—mediated ECL, and signal is net of background. Error bars are standard error. c) Electron micrograph of plant-produced τGFP particles in the presence of GFP, purified by sucrose cushion and size exclusion chromatography. Scale bar 100 nm.
    Raw Fluorescence, supplied by Affibody, used in various techniques. Bioz Stars score: 78/100, based on 3 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/raw fluorescence/product/Affibody
    Average 78 stars, based on 3 article reviews
    Price from $9.99 to $1999.99
    raw fluorescence - by Bioz Stars, 2020-02
    78/100 stars
      Buy from Supplier

    91
    Affibody plasma membrane
    Plant-produced τGFP particles bind GFP. a) Ultracentrifuge tubes containing sucrose cushions photographed under UV light after ultracentrifugation. GFP-associated fluorescence remains in the supernatant when GFP-containing plant lysate is centrifuged alone or mixed with tEL-containing plant lysate; but migrates through the cushion when GFP-containing and τGFP-containing plant lysates are mixed. b) Detection of GFP by sandwich ELISA, after coating wells with τGFP (green), τglyc (orange) or an anti-GFP <t>polyclonal</t> IgG (blue) and adding GFP to the wells at four different concentrations after blocking. Detection is horseradish peroxidase—mediated ECL, and signal is net of background. Error bars are standard error. c) Electron micrograph of plant-produced τGFP particles in the presence of GFP, purified by sucrose cushion and size exclusion chromatography. Scale bar 100 nm.
    Plasma Membrane, supplied by Affibody, used in various techniques. Bioz Stars score: 91/100, based on 19 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/plasma membrane/product/Affibody
    Average 91 stars, based on 19 article reviews
    Price from $9.99 to $1999.99
    plasma membrane - by Bioz Stars, 2020-02
    91/100 stars
      Buy from Supplier

    86
    Affibody anti fluorescein scfv
    Plant-produced τGFP particles bind GFP. a) Ultracentrifuge tubes containing sucrose cushions photographed under UV light after ultracentrifugation. GFP-associated fluorescence remains in the supernatant when GFP-containing plant lysate is centrifuged alone or mixed with tEL-containing plant lysate; but migrates through the cushion when GFP-containing and τGFP-containing plant lysates are mixed. b) Detection of GFP by sandwich ELISA, after coating wells with τGFP (green), τglyc (orange) or an anti-GFP <t>polyclonal</t> IgG (blue) and adding GFP to the wells at four different concentrations after blocking. Detection is horseradish peroxidase—mediated ECL, and signal is net of background. Error bars are standard error. c) Electron micrograph of plant-produced τGFP particles in the presence of GFP, purified by sucrose cushion and size exclusion chromatography. Scale bar 100 nm.
    Anti Fluorescein Scfv, supplied by Affibody, used in various techniques. Bioz Stars score: 86/100, based on 11 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/anti fluorescein scfv/product/Affibody
    Average 86 stars, based on 11 article reviews
    Price from $9.99 to $1999.99
    anti fluorescein scfv - by Bioz Stars, 2020-02
    86/100 stars
      Buy from Supplier

    Image Search Results


    Effect of logD and charge on affibody conjugate mobility. Plots of mean instantaneous D fit for different anti-EGFR Affibody conjugates vs charge at pH 7.4 ( A ), and logD ( B ). C) Plot of spot density for selected anti-EGFR Affibody conjugates vs charge at logD. Each datapoint corresponds to mean ± SEM of at least 10 independent areas. Lines show linear regression fit to the data, R 2 values indicating goodness of fit. Alexa 555 is not included in this figure as the structure is not published and charge and logD values are unavailable.

    Journal: PLoS ONE

    Article Title: Hydrophobic Fluorescent Probes Introduce Artifacts into Single Molecule Tracking Experiments Due to Non-Specific Binding

    doi: 10.1371/journal.pone.0074200

    Figure Lengend Snippet: Effect of logD and charge on affibody conjugate mobility. Plots of mean instantaneous D fit for different anti-EGFR Affibody conjugates vs charge at pH 7.4 ( A ), and logD ( B ). C) Plot of spot density for selected anti-EGFR Affibody conjugates vs charge at logD. Each datapoint corresponds to mean ± SEM of at least 10 independent areas. Lines show linear regression fit to the data, R 2 values indicating goodness of fit. Alexa 555 is not included in this figure as the structure is not published and charge and logD values are unavailable.

    Article Snippet: In our previous work we used mean instantaneous diffusion coefficient (D ) values as a measure of the mobility of dye conjugates bound to receptors in the plasma membrane of T47D cells, and showed that anti-EGFR affibody conjugated to Alexa Fluor 488 exhibits similar levels of mobility when bound to EGFR in cells to endogenously labelled EGFR-GFP.

    Techniques:

    PET images, summed 30–60 min, and TACs from a Balbc nu/nu mouse (prone) bearing tumors ( white arrows ): a one A431 xenograft (1 × 10 7 cells, 15 days) or b two A431 xenografts ( left : 1 × 10 7 cells, 28 days; right : 1 × 10 7 cells, 25 days). Comparison A shows a 7-times higher uptake with targeting [methyl- 11 C]-Z EGFR:2377 -ST-CH 3 compared to the non-targeting [methyl- 11 C]-Z Taq:3638 -ST-CH 3 . Comparison B illustrates uptake of the targeting Affibody increasing as the tumors grow from time from inoculation

    Journal: EJNMMI Research

    Article Title: Preclinical PET imaging of EGFR levels: pairing a targeting with a non-targeting Sel-tagged Affibody-based tracer to estimate the specific uptake

    doi: 10.1186/s13550-016-0213-8

    Figure Lengend Snippet: PET images, summed 30–60 min, and TACs from a Balbc nu/nu mouse (prone) bearing tumors ( white arrows ): a one A431 xenograft (1 × 10 7 cells, 15 days) or b two A431 xenografts ( left : 1 × 10 7 cells, 28 days; right : 1 × 10 7 cells, 25 days). Comparison A shows a 7-times higher uptake with targeting [methyl- 11 C]-Z EGFR:2377 -ST-CH 3 compared to the non-targeting [methyl- 11 C]-Z Taq:3638 -ST-CH 3 . Comparison B illustrates uptake of the targeting Affibody increasing as the tumors grow from time from inoculation

    Article Snippet: Preparation and labeling of the Sel-tagged Affibody molecules The Sel-tagged Affibody molecules were successfully obtained by expression in E. coli as C-terminal fusions to green fluorescent protein (GFP), then recovered with immobilized metal ion affinity chromatography (IMAC), released by tobacco etch virus (TEV)-protease cleavage, and purified by high-performance liquid chromatography (HPLC).

    Techniques: Positron Emission Tomography

    PET images, summed 30–60 min, and TACs from a SCID mouse (prone) bearing tumors ( white arrows ): a one FaDu xenograft (1 × 10 6 cells, 12 days) or b two FaDu xenografts ( left : (1 × 10 6 cells, 12 days); right : (0.5 × 10 6 cells, 12 days). Comparison A illustrates the higher uptake with targeting [methyl- 11 C]-Z EGFR:2377 -ST-CH 3 but with a ≈60 % non-targeting uptake of [methyl- 11 C]-Z Taq:3638 -ST-CH 3 . Comparison B illustrates the visually discernable heterogeneous uptake of the targeting Affibody in the larger tumor on the left. SUV mean is affected by whether the entire (1) or only central ROI (2) of the left tumor is used

    Journal: EJNMMI Research

    Article Title: Preclinical PET imaging of EGFR levels: pairing a targeting with a non-targeting Sel-tagged Affibody-based tracer to estimate the specific uptake

    doi: 10.1186/s13550-016-0213-8

    Figure Lengend Snippet: PET images, summed 30–60 min, and TACs from a SCID mouse (prone) bearing tumors ( white arrows ): a one FaDu xenograft (1 × 10 6 cells, 12 days) or b two FaDu xenografts ( left : (1 × 10 6 cells, 12 days); right : (0.5 × 10 6 cells, 12 days). Comparison A illustrates the higher uptake with targeting [methyl- 11 C]-Z EGFR:2377 -ST-CH 3 but with a ≈60 % non-targeting uptake of [methyl- 11 C]-Z Taq:3638 -ST-CH 3 . Comparison B illustrates the visually discernable heterogeneous uptake of the targeting Affibody in the larger tumor on the left. SUV mean is affected by whether the entire (1) or only central ROI (2) of the left tumor is used

    Article Snippet: Preparation and labeling of the Sel-tagged Affibody molecules The Sel-tagged Affibody molecules were successfully obtained by expression in E. coli as C-terminal fusions to green fluorescent protein (GFP), then recovered with immobilized metal ion affinity chromatography (IMAC), released by tobacco etch virus (TEV)-protease cleavage, and purified by high-performance liquid chromatography (HPLC).

    Techniques: Positron Emission Tomography

    Plant-produced τGFP particles bind GFP. a) Ultracentrifuge tubes containing sucrose cushions photographed under UV light after ultracentrifugation. GFP-associated fluorescence remains in the supernatant when GFP-containing plant lysate is centrifuged alone or mixed with tEL-containing plant lysate; but migrates through the cushion when GFP-containing and τGFP-containing plant lysates are mixed. b) Detection of GFP by sandwich ELISA, after coating wells with τGFP (green), τglyc (orange) or an anti-GFP polyclonal IgG (blue) and adding GFP to the wells at four different concentrations after blocking. Detection is horseradish peroxidase—mediated ECL, and signal is net of background. Error bars are standard error. c) Electron micrograph of plant-produced τGFP particles in the presence of GFP, purified by sucrose cushion and size exclusion chromatography. Scale bar 100 nm.

    Journal: PLoS ONE

    Article Title: Tandem Fusion of Hepatitis B Core Antigen Allows Assembly of Virus-Like Particles in Bacteria and Plants with Enhanced Capacity to Accommodate Foreign Proteins

    doi: 10.1371/journal.pone.0120751

    Figure Lengend Snippet: Plant-produced τGFP particles bind GFP. a) Ultracentrifuge tubes containing sucrose cushions photographed under UV light after ultracentrifugation. GFP-associated fluorescence remains in the supernatant when GFP-containing plant lysate is centrifuged alone or mixed with tEL-containing plant lysate; but migrates through the cushion when GFP-containing and τGFP-containing plant lysates are mixed. b) Detection of GFP by sandwich ELISA, after coating wells with τGFP (green), τglyc (orange) or an anti-GFP polyclonal IgG (blue) and adding GFP to the wells at four different concentrations after blocking. Detection is horseradish peroxidase—mediated ECL, and signal is net of background. Error bars are standard error. c) Electron micrograph of plant-produced τGFP particles in the presence of GFP, purified by sucrose cushion and size exclusion chromatography. Scale bar 100 nm.

    Article Snippet: These particles, along with a goat polyclonal antibody specific for GFP (Abcam ab6673; positive control), were used to coat the wells of a 96-well plate (F96 Maxisorp, Nunc) at 25 ng/well.

    Techniques: Produced, Fluorescence, Sandwich ELISA, Blocking Assay, Purification, Size-exclusion Chromatography