genotyping Search Results


  • Logo
  • About
  • News
  • Press Release
  • Team
  • Advisors
  • Partners
  • Contact
  • Bioz Stars
  • Bioz vStars
  • 91
    Thermo Fisher rs12007907 genotyping
    Rs12007907 Genotyping, supplied by Thermo Fisher, used in various techniques. Bioz Stars score: 91/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/rs12007907 genotyping/product/Thermo Fisher
    Average 91 stars, based on 1 article reviews
    Price from $9.99 to $1999.99
    rs12007907 genotyping - by Bioz Stars, 2020-08
    91/100 stars
      Buy from Supplier

    93
    Thermo Fisher rs12979860 genotyping
    Rs12979860 Genotyping, supplied by Thermo Fisher, used in various techniques. Bioz Stars score: 93/100, based on 26 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/rs12979860 genotyping/product/Thermo Fisher
    Average 93 stars, based on 26 article reviews
    Price from $9.99 to $1999.99
    rs12979860 genotyping - by Bioz Stars, 2020-08
    93/100 stars
      Buy from Supplier

    90
    Thermo Fisher rs17315309 genotyping
    Rs17315309 Genotyping, supplied by Thermo Fisher, used in various techniques. Bioz Stars score: 90/100, based on 2 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/rs17315309 genotyping/product/Thermo Fisher
    Average 90 stars, based on 2 article reviews
    Price from $9.99 to $1999.99
    rs17315309 genotyping - by Bioz Stars, 2020-08
    90/100 stars
      Buy from Supplier

    85
    Thermo Fisher rs11555236 genotypes
    SIRT3 Western blotting in peripheral blood mononucleate cells. To assess a possible functional correlation of <t>rs11555236</t> variants with SIRT3 expression, we selected a group of subjects from the TRELONG study, balanced for gender, and evaluated SIRT3 protein
    Rs11555236 Genotypes, supplied by Thermo Fisher, used in various techniques. Bioz Stars score: 85/100, based on 4 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/rs11555236 genotypes/product/Thermo Fisher
    Average 85 stars, based on 4 article reviews
    Price from $9.99 to $1999.99
    rs11555236 genotypes - by Bioz Stars, 2020-08
    85/100 stars
      Buy from Supplier

    88
    Thermo Fisher rs12980275 genotype
    Agarose gel electrophoresis analysis of rs8099917 and <t>rs12980275</t> polymorphism in IL28 gene. A: TT genotype produced only one 400 bp band, GG genotype produced 2 small fragments at 234 and 166 bp, respectively, and TG genotype produced 3 bands: 400, 234, and 166 bp, respectively. M Lane represents the 50 bp molecular marker. B: The AA genotype produced two bands 178, 115 bp, the AG genotype produced 3 bands: 178, 148, and 115 bp, the GG genotype produced 2 small fragments at 148, 115 (30 bp band not appear) respectively. M lane represents the 50bp molecular marker.
    Rs12980275 Genotype, supplied by Thermo Fisher, used in various techniques. Bioz Stars score: 88/100, based on 17 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/rs12980275 genotype/product/Thermo Fisher
    Average 88 stars, based on 17 article reviews
    Price from $9.99 to $1999.99
    rs12980275 genotype - by Bioz Stars, 2020-08
    88/100 stars
      Buy from Supplier

    85
    Thermo Fisher rs12203592 genotypes
    Self-Reported Mole Scores in Queensland Melanoma Cases versus Age and Genotype at <t>rs12203592</t> in IRF4
    Rs12203592 Genotypes, supplied by Thermo Fisher, used in various techniques. Bioz Stars score: 85/100, based on 3 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/rs12203592 genotypes/product/Thermo Fisher
    Average 85 stars, based on 3 article reviews
    Price from $9.99 to $1999.99
    rs12203592 genotypes - by Bioz Stars, 2020-08
    85/100 stars
      Buy from Supplier

    91
    Thermo Fisher rs34457782 genotype
    Self-Reported Mole Scores in Queensland Melanoma Cases versus Age and Genotype at <t>rs12203592</t> in IRF4
    Rs34457782 Genotype, supplied by Thermo Fisher, used in various techniques. Bioz Stars score: 91/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/rs34457782 genotype/product/Thermo Fisher
    Average 91 stars, based on 1 article reviews
    Price from $9.99 to $1999.99
    rs34457782 genotype - by Bioz Stars, 2020-08
    91/100 stars
      Buy from Supplier

    95
    Thermo Fisher taqman openarray genotyping genotyping
    Flowchart showing the study design and analysis. Patients were classified according to their disease course into benign and aggressive MS, as described in the Methods. By means of exome sequencing, a total of 915 single-nucleotide polymorphisms (SNPs) were identified from 10 MS patients with benign and 10 with aggressive disease courses as being differentially distributed between both groups (discovery cohort). After applying several selection criteria on the list of 915 SNPs including odds ratio difference, phenotype prevalence, number of statistically significant SNPs per gene, type and variant effects on the predicted protein, and relevance of target genes to MS, a total of 16 SNPs were chosen for further validation in two independent cohorts of patients also classified into benign and aggressive phenotypes. The first validation cohort comprised 194 MS patients, 107 with benign and 87 with aggressive disease courses, and <t>genotyping</t> was conducting using an <t>OpenArray</t> technology. The second validation cohort consisted of 257 MS patients, 224 with benign and 33 with aggressive disease courses, and genotyping was performed on a MassArray iPLEX platform. Finally, a meta-analysis was performed in the two validation cohorts
    Taqman Openarray Genotyping Genotyping, supplied by Thermo Fisher, used in various techniques. Bioz Stars score: 95/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/taqman openarray genotyping genotyping/product/Thermo Fisher
    Average 95 stars, based on 1 article reviews
    Price from $9.99 to $1999.99
    taqman openarray genotyping genotyping - by Bioz Stars, 2020-08
    95/100 stars
      Buy from Supplier

    93
    Thermo Fisher hsd17b13 rs72613567 genotyping
    Flowchart showing the study design and analysis. Patients were classified according to their disease course into benign and aggressive MS, as described in the Methods. By means of exome sequencing, a total of 915 single-nucleotide polymorphisms (SNPs) were identified from 10 MS patients with benign and 10 with aggressive disease courses as being differentially distributed between both groups (discovery cohort). After applying several selection criteria on the list of 915 SNPs including odds ratio difference, phenotype prevalence, number of statistically significant SNPs per gene, type and variant effects on the predicted protein, and relevance of target genes to MS, a total of 16 SNPs were chosen for further validation in two independent cohorts of patients also classified into benign and aggressive phenotypes. The first validation cohort comprised 194 MS patients, 107 with benign and 87 with aggressive disease courses, and <t>genotyping</t> was conducting using an <t>OpenArray</t> technology. The second validation cohort consisted of 257 MS patients, 224 with benign and 33 with aggressive disease courses, and genotyping was performed on a MassArray iPLEX platform. Finally, a meta-analysis was performed in the two validation cohorts
    Hsd17b13 Rs72613567 Genotyping, supplied by Thermo Fisher, used in various techniques. Bioz Stars score: 93/100, based on 2 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/hsd17b13 rs72613567 genotyping/product/Thermo Fisher
    Average 93 stars, based on 2 article reviews
    Price from $9.99 to $1999.99
    hsd17b13 rs72613567 genotyping - by Bioz Stars, 2020-08
    93/100 stars
      Buy from Supplier

    90
    Thermo Fisher rs12769205 genotypes
    IGV visualization of human liver CYP2C19 mRNA. RNA-Seq results for human livers that were heterozygous (half-filled box) or homozygous wild type (open box). (A) <t>rs12769205</t> were visualized across CYP2C19 exons 2-Exon3; and (B) rs4244285 were visualized
    Rs12769205 Genotypes, supplied by Thermo Fisher, used in various techniques. Bioz Stars score: 90/100, based on 3 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/rs12769205 genotypes/product/Thermo Fisher
    Average 90 stars, based on 3 article reviews
    Price from $9.99 to $1999.99
    rs12769205 genotypes - by Bioz Stars, 2020-08
    90/100 stars
      Buy from Supplier

    90
    Thermo Fisher rs80999917 genotypes
    IGV visualization of human liver CYP2C19 mRNA. RNA-Seq results for human livers that were heterozygous (half-filled box) or homozygous wild type (open box). (A) <t>rs12769205</t> were visualized across CYP2C19 exons 2-Exon3; and (B) rs4244285 were visualized
    Rs80999917 Genotypes, supplied by Thermo Fisher, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/rs80999917 genotypes/product/Thermo Fisher
    Average 90 stars, based on 1 article reviews
    Price from $9.99 to $1999.99
    rs80999917 genotypes - by Bioz Stars, 2020-08
    90/100 stars
      Buy from Supplier

    91
    Thermo Fisher rs12041331 genotype
    IGV visualization of human liver CYP2C19 mRNA. RNA-Seq results for human livers that were heterozygous (half-filled box) or homozygous wild type (open box). (A) <t>rs12769205</t> were visualized across CYP2C19 exons 2-Exon3; and (B) rs4244285 were visualized
    Rs12041331 Genotype, supplied by Thermo Fisher, used in various techniques. Bioz Stars score: 91/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/rs12041331 genotype/product/Thermo Fisher
    Average 91 stars, based on 1 article reviews
    Price from $9.99 to $1999.99
    rs12041331 genotype - by Bioz Stars, 2020-08
    91/100 stars
      Buy from Supplier

    90
    Thermo Fisher gadl1 rs17026688 genotyping
    IGV visualization of human liver CYP2C19 mRNA. RNA-Seq results for human livers that were heterozygous (half-filled box) or homozygous wild type (open box). (A) <t>rs12769205</t> were visualized across CYP2C19 exons 2-Exon3; and (B) rs4244285 were visualized
    Gadl1 Rs17026688 Genotyping, supplied by Thermo Fisher, used in various techniques. Bioz Stars score: 90/100, based on 2 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/gadl1 rs17026688 genotyping/product/Thermo Fisher
    Average 90 stars, based on 2 article reviews
    Price from $9.99 to $1999.99
    gadl1 rs17026688 genotyping - by Bioz Stars, 2020-08
    90/100 stars
      Buy from Supplier

    92
    Thermo Fisher rs12979860 genotypes
    3.2. Modification of IL-28B SNP rs8099917 and <t>rs12979860</t> Genotypes after LDLT
    Rs12979860 Genotypes, supplied by Thermo Fisher, used in various techniques. Bioz Stars score: 92/100, based on 2 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/rs12979860 genotypes/product/Thermo Fisher
    Average 92 stars, based on 2 article reviews
    Price from $9.99 to $1999.99
    rs12979860 genotypes - by Bioz Stars, 2020-08
    92/100 stars
      Buy from Supplier

    85
    Thermo Fisher rs17809012 genotype
    3.2. Modification of IL-28B SNP rs8099917 and <t>rs12979860</t> Genotypes after LDLT
    Rs17809012 Genotype, supplied by Thermo Fisher, used in various techniques. Bioz Stars score: 85/100, based on 5 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/rs17809012 genotype/product/Thermo Fisher
    Average 85 stars, based on 5 article reviews
    Price from $9.99 to $1999.99
    rs17809012 genotype - by Bioz Stars, 2020-08
    85/100 stars
      Buy from Supplier

    86
    Thermo Fisher rs12979860 snp genotyping
    3.2. Modification of IL-28B SNP rs8099917 and <t>rs12979860</t> Genotypes after LDLT
    Rs12979860 Snp Genotyping, supplied by Thermo Fisher, used in various techniques. Bioz Stars score: 86/100, based on 3 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/rs12979860 snp genotyping/product/Thermo Fisher
    Average 86 stars, based on 3 article reviews
    Price from $9.99 to $1999.99
    rs12979860 snp genotyping - by Bioz Stars, 2020-08
    86/100 stars
      Buy from Supplier

    92
    Thermo Fisher genotyper
    3.2. Modification of IL-28B SNP rs8099917 and <t>rs12979860</t> Genotypes after LDLT
    Genotyper, supplied by Thermo Fisher, used in various techniques. Bioz Stars score: 92/100, based on 803 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/genotyper/product/Thermo Fisher
    Average 92 stars, based on 803 article reviews
    Price from $9.99 to $1999.99
    genotyper - by Bioz Stars, 2020-08
    92/100 stars
      Buy from Supplier

    92
    Illumina Inc genotyping
    <t>Genotyping</t> and characterization of linkage disequilibrium
    Genotyping, supplied by Illumina Inc, used in various techniques. Bioz Stars score: 92/100, based on 5933 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/genotyping/product/Illumina Inc
    Average 92 stars, based on 5933 article reviews
    Price from $9.99 to $1999.99
    genotyping - by Bioz Stars, 2020-08
    92/100 stars
      Buy from Supplier

    92
    Oxford Nanopore genotyping
    <t>Genotyping</t> and characterization of linkage disequilibrium
    Genotyping, supplied by Oxford Nanopore, used in various techniques. Bioz Stars score: 92/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/genotyping/product/Oxford Nanopore
    Average 92 stars, based on 1 article reviews
    Price from $9.99 to $1999.99
    genotyping - by Bioz Stars, 2020-08
    92/100 stars
      Buy from Supplier

    93
    QuintilesIMS genotyping
    <t>Genotyping</t> and characterization of linkage disequilibrium
    Genotyping, supplied by QuintilesIMS, used in various techniques. Bioz Stars score: 93/100, based on 5 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/genotyping/product/QuintilesIMS
    Average 93 stars, based on 5 article reviews
    Price from $9.99 to $1999.99
    genotyping - by Bioz Stars, 2020-08
    93/100 stars
      Buy from Supplier

    94
    Sequenom genotyping
    Genomic structure and pair-wise marker LD in ADIPOR1 ( a ) and ADIPOR2 ( b ). The location of SNPs identified in this study and/or genotyped is represented along the gene ( bold type : SNPs selected for <t>genotyping,</t> and failed or monomorphic SNPs ). Exons are represented in boxes ( black for coding and open for untranslated ). Introns and flanking sequences appear as lines . The pair-wise marker LD measured by r 2 statistics is shown below the genomic structures and indicated by the shade of grey blocks ( white to black ) and the r 2 value. a , b , isoforms a and b in ADIPOR2
    Genotyping, supplied by Sequenom, used in various techniques. Bioz Stars score: 94/100, based on 3330 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/genotyping/product/Sequenom
    Average 94 stars, based on 3330 article reviews
    Price from $9.99 to $1999.99
    genotyping - by Bioz Stars, 2020-08
    94/100 stars
      Buy from Supplier

    94
    Thermo Fisher genotyping
    SNP calling pattern, genome density and polymorphism of SNPs in ‘Reference Set’ using Axiom_ Arachis SNP array. This figure shows ( a , b ) monomorphic SNPs identified in the <t>genotyping</t> data, ( c ) polymorphic SNPs without heterozygosity i.e., homozygous SNPs, ( d ) polymorphic SNPs with heterozygosity, ( e ) genome-wide distribution of SNPs, and ( f ) pseudomolecule-wise distribution of SNPs on array and polymorphic SNPs in the ‘Reference Set’.
    Genotyping, supplied by Thermo Fisher, used in various techniques. Bioz Stars score: 94/100, based on 12616 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/genotyping/product/Thermo Fisher
    Average 94 stars, based on 12616 article reviews
    Price from $9.99 to $1999.99
    genotyping - by Bioz Stars, 2020-08
    94/100 stars
      Buy from Supplier

    94
    Transnetyx genotyping
    FOXC2 is able to functionally substitute for FOXC1 during lymphatic valve development. ( a ) Schematic representation of the targeting vector and targeted allele. The entire protein coding region of Foxc1 is replaced with that of Foxc2 . ACN, self-excision cassette including Cre driven by the testis-specific promoter. ( b ) Southern blot analysis to detect double-resistant ES cell colonies using 5’ and 3’ probes. ( c ) PCR <t>genotyping</t> of F1 heterozygotes to detect the Foxc1 c2 allele. ( d, e ) Representative images of lymphatic valves in mesenteric collecting vessels immunostained with antibodies targeted to FOXC1 and VEGFR-3 from P12 P6 Foxc1 +/+ ( d ) and Foxc1 c2/c2 ( e ) mice. Scale bars are 25 µm. (f – h) Representative images of lymphatic valves in mesenteric collecting vessels immunostained with antibodies targeted to FOXC1, FOXC2, and VE-Cadherin from P6 Foxc1 +/+ ( f ) , Foxc1 c2/+ ( g ), and Foxc1 c2/c2 mice ( h ). Scale bars are 50 μm. (i – k) Representative images of the mesenteric vasculature immunostained with antibodies targeted to PROX1 and CD31 in P6 Foxc1 +/+ ( i ) , Foxc1 c2/+ ( j ), and Foxc1 c2/c2 mice ( k ). Scale bars are 200 μm. (l – n) Representative images of P6 mesenteric vasculature from P6 Foxc1 +/+ ( l ) , Foxc1 c2/+ ( m ), and Foxc1 c2/c2 mice ( n ) immunostained with antibodies targeted to FOXC1, FOXC2, and VE-Cadherin show gradual loss of FOXC1 expression in the blood and lymphatic vasculature and smooth muscle cells and conversely the increase of FOXC2 expression in blood vasculature and smooth muscle. Scale bars are 200 μm. ( o ) Quantification of total lymphatic valve number in lymphatic collecting vessels of P6 Foxc1 +/+ , Foxc1 c2/+ , and Foxc1 c2/c2 individuals. N = 6 for Foxc1 +/+ , N = 8 for Foxc1 c2/+ , and N = 8 for Foxc1 c2/c2 individuals. ( p ) Percentage of mature and immature lymphatic valves normalized to total valves counted in P6 Foxc1 +/+ , Foxc1 c2/+ , and Foxc1 c2/c2 individuals. Data are presented as mean (± SD) and analyzed using Student’s t-test. NS denotes no significance.
    Genotyping, supplied by Transnetyx, used in various techniques. Bioz Stars score: 94/100, based on 341 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/genotyping/product/Transnetyx
    Average 94 stars, based on 341 article reviews
    Price from $9.99 to $1999.99
    genotyping - by Bioz Stars, 2020-08
    94/100 stars
      Buy from Supplier

    90
    Thermo Fisher snp genotyping rs12979860
    FOXC2 is able to functionally substitute for FOXC1 during lymphatic valve development. ( a ) Schematic representation of the targeting vector and targeted allele. The entire protein coding region of Foxc1 is replaced with that of Foxc2 . ACN, self-excision cassette including Cre driven by the testis-specific promoter. ( b ) Southern blot analysis to detect double-resistant ES cell colonies using 5’ and 3’ probes. ( c ) PCR <t>genotyping</t> of F1 heterozygotes to detect the Foxc1 c2 allele. ( d, e ) Representative images of lymphatic valves in mesenteric collecting vessels immunostained with antibodies targeted to FOXC1 and VEGFR-3 from P12 P6 Foxc1 +/+ ( d ) and Foxc1 c2/c2 ( e ) mice. Scale bars are 25 µm. (f – h) Representative images of lymphatic valves in mesenteric collecting vessels immunostained with antibodies targeted to FOXC1, FOXC2, and VE-Cadherin from P6 Foxc1 +/+ ( f ) , Foxc1 c2/+ ( g ), and Foxc1 c2/c2 mice ( h ). Scale bars are 50 μm. (i – k) Representative images of the mesenteric vasculature immunostained with antibodies targeted to PROX1 and CD31 in P6 Foxc1 +/+ ( i ) , Foxc1 c2/+ ( j ), and Foxc1 c2/c2 mice ( k ). Scale bars are 200 μm. (l – n) Representative images of P6 mesenteric vasculature from P6 Foxc1 +/+ ( l ) , Foxc1 c2/+ ( m ), and Foxc1 c2/c2 mice ( n ) immunostained with antibodies targeted to FOXC1, FOXC2, and VE-Cadherin show gradual loss of FOXC1 expression in the blood and lymphatic vasculature and smooth muscle cells and conversely the increase of FOXC2 expression in blood vasculature and smooth muscle. Scale bars are 200 μm. ( o ) Quantification of total lymphatic valve number in lymphatic collecting vessels of P6 Foxc1 +/+ , Foxc1 c2/+ , and Foxc1 c2/c2 individuals. N = 6 for Foxc1 +/+ , N = 8 for Foxc1 c2/+ , and N = 8 for Foxc1 c2/c2 individuals. ( p ) Percentage of mature and immature lymphatic valves normalized to total valves counted in P6 Foxc1 +/+ , Foxc1 c2/+ , and Foxc1 c2/c2 individuals. Data are presented as mean (± SD) and analyzed using Student’s t-test. NS denotes no significance.
    Snp Genotyping Rs12979860, supplied by Thermo Fisher, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/snp genotyping rs12979860/product/Thermo Fisher
    Average 90 stars, based on 1 article reviews
    Price from $9.99 to $1999.99
    snp genotyping rs12979860 - by Bioz Stars, 2020-08
    90/100 stars
      Buy from Supplier

    94
    Roche genotyping
    Characterisation of MCK-tTA-hYAP1 S127A mouse model. A) Schematic outline of the inducible ‘Tet-Off’ transgenic system. B) <t>Genotyping</t> of MCK-tTA and TRE-hYAP1 S127A alleles in single and double transgenic mice. C) Yap protein levels in skeletal muscles of transgenic mice of indicated genotypes following 25 days with (+) or without (−) doxycycline (dox). Tibialis anterior (TA), gastrocnemius (Gas) and extensor digitorum longus (EDL). D) Muscle specific expression of hYAP mRNA in MCK-tTA-hYAP1 S127A mice 25 days after doxycycline withdrawal. E) Time course of Yap protein expression in tibialis anterior of MCK-tTA-hYAP1 S127A mice following doxycycline removal.
    Genotyping, supplied by Roche, used in various techniques. Bioz Stars score: 94/100, based on 1423 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/genotyping/product/Roche
    Average 94 stars, based on 1423 article reviews
    Price from $9.99 to $1999.99
    genotyping - by Bioz Stars, 2020-08
    94/100 stars
      Buy from Supplier

    Image Search Results


    SIRT3 Western blotting in peripheral blood mononucleate cells. To assess a possible functional correlation of rs11555236 variants with SIRT3 expression, we selected a group of subjects from the TRELONG study, balanced for gender, and evaluated SIRT3 protein

    Journal: Age

    Article Title: Modulation of human longevity by SIRT3 single nucleotide polymorphisms in the prospective study “Treviso Longeva (TRELONG)”

    doi: 10.1007/s11357-013-9559-2

    Figure Lengend Snippet: SIRT3 Western blotting in peripheral blood mononucleate cells. To assess a possible functional correlation of rs11555236 variants with SIRT3 expression, we selected a group of subjects from the TRELONG study, balanced for gender, and evaluated SIRT3 protein

    Article Snippet: To assess rs3825075, rs4980329, and rs11555236 genotypes, a gDNA aliquot (about 20 ng) was used in an allelic discrimination assay using a real-time PCR apparatus and TaqMan technology according to the manufacturer's instructions (Applied Biosystems, Foster City, CA).

    Techniques: Western Blot, Functional Assay, Expressing

    Agarose gel electrophoresis analysis of rs8099917 and rs12980275 polymorphism in IL28 gene. A: TT genotype produced only one 400 bp band, GG genotype produced 2 small fragments at 234 and 166 bp, respectively, and TG genotype produced 3 bands: 400, 234, and 166 bp, respectively. M Lane represents the 50 bp molecular marker. B: The AA genotype produced two bands 178, 115 bp, the AG genotype produced 3 bands: 178, 148, and 115 bp, the GG genotype produced 2 small fragments at 148, 115 (30 bp band not appear) respectively. M lane represents the 50bp molecular marker.

    Journal: Journal of Biomedical Research

    Article Title: Impact of IL28B gene polymorphisms rs8099917 and rs12980275 on response to pegylated interferon-α/ribavirin therapy in chronic hepatitis C genotype 4 patients

    doi: 10.7555/JBR.30.20150002

    Figure Lengend Snippet: Agarose gel electrophoresis analysis of rs8099917 and rs12980275 polymorphism in IL28 gene. A: TT genotype produced only one 400 bp band, GG genotype produced 2 small fragments at 234 and 166 bp, respectively, and TG genotype produced 3 bands: 400, 234, and 166 bp, respectively. M Lane represents the 50 bp molecular marker. B: The AA genotype produced two bands 178, 115 bp, the AG genotype produced 3 bands: 178, 148, and 115 bp, the GG genotype produced 2 small fragments at 148, 115 (30 bp band not appear) respectively. M lane represents the 50bp molecular marker.

    Article Snippet: For RFLP assay for rs12980275 genotype, amplicons were digested with 2 U BseL I restriction endonuclease (Fermentas) at 55°C for 12 hours.

    Techniques: Agarose Gel Electrophoresis, Produced, Marker

    Self-Reported Mole Scores in Queensland Melanoma Cases versus Age and Genotype at rs12203592 in IRF4

    Journal: American Journal of Human Genetics

    Article Title: IRF4 Variants Have Age-Specific Effects on Nevus Count and Predispose to Melanoma

    doi: 10.1016/j.ajhg.2010.05.017

    Figure Lengend Snippet: Self-Reported Mole Scores in Queensland Melanoma Cases versus Age and Genotype at rs12203592 in IRF4

    Article Snippet: The rs12203592 genotypes were assessed with the use of the Taqman SNP genotyping assay C_31918199_10 (Applied Biosystems).

    Techniques:

    Adolescent Macular and Papular Mole Counts versus rs12203592 Genotype, Stratified on the Four Levels of Freckling Score

    Journal: American Journal of Human Genetics

    Article Title: IRF4 Variants Have Age-Specific Effects on Nevus Count and Predispose to Melanoma

    doi: 10.1016/j.ajhg.2010.05.017

    Figure Lengend Snippet: Adolescent Macular and Papular Mole Counts versus rs12203592 Genotype, Stratified on the Four Levels of Freckling Score

    Article Snippet: The rs12203592 genotypes were assessed with the use of the Taqman SNP genotyping assay C_31918199_10 (Applied Biosystems).

    Techniques:

    Combined Analysis of Melanoma Case-Control Data from Australia, the UK, and Sweden for rs12203592 by Tumor Site, Showing the Strongest Association with Melanoma on the Trunk: OR = 1.32, p = 2.5 × 10 −5

    Journal: American Journal of Human Genetics

    Article Title: IRF4 Variants Have Age-Specific Effects on Nevus Count and Predispose to Melanoma

    doi: 10.1016/j.ajhg.2010.05.017

    Figure Lengend Snippet: Combined Analysis of Melanoma Case-Control Data from Australia, the UK, and Sweden for rs12203592 by Tumor Site, Showing the Strongest Association with Melanoma on the Trunk: OR = 1.32, p = 2.5 × 10 −5

    Article Snippet: The rs12203592 genotypes were assessed with the use of the Taqman SNP genotyping assay C_31918199_10 (Applied Biosystems).

    Techniques:

    Flowchart showing the study design and analysis. Patients were classified according to their disease course into benign and aggressive MS, as described in the Methods. By means of exome sequencing, a total of 915 single-nucleotide polymorphisms (SNPs) were identified from 10 MS patients with benign and 10 with aggressive disease courses as being differentially distributed between both groups (discovery cohort). After applying several selection criteria on the list of 915 SNPs including odds ratio difference, phenotype prevalence, number of statistically significant SNPs per gene, type and variant effects on the predicted protein, and relevance of target genes to MS, a total of 16 SNPs were chosen for further validation in two independent cohorts of patients also classified into benign and aggressive phenotypes. The first validation cohort comprised 194 MS patients, 107 with benign and 87 with aggressive disease courses, and genotyping was conducting using an OpenArray technology. The second validation cohort consisted of 257 MS patients, 224 with benign and 33 with aggressive disease courses, and genotyping was performed on a MassArray iPLEX platform. Finally, a meta-analysis was performed in the two validation cohorts

    Journal: Journal of Neuroinflammation

    Article Title: Exome sequencing study in patients with multiple sclerosis reveals variants associated with disease course

    doi: 10.1186/s12974-018-1307-1

    Figure Lengend Snippet: Flowchart showing the study design and analysis. Patients were classified according to their disease course into benign and aggressive MS, as described in the Methods. By means of exome sequencing, a total of 915 single-nucleotide polymorphisms (SNPs) were identified from 10 MS patients with benign and 10 with aggressive disease courses as being differentially distributed between both groups (discovery cohort). After applying several selection criteria on the list of 915 SNPs including odds ratio difference, phenotype prevalence, number of statistically significant SNPs per gene, type and variant effects on the predicted protein, and relevance of target genes to MS, a total of 16 SNPs were chosen for further validation in two independent cohorts of patients also classified into benign and aggressive phenotypes. The first validation cohort comprised 194 MS patients, 107 with benign and 87 with aggressive disease courses, and genotyping was conducting using an OpenArray technology. The second validation cohort consisted of 257 MS patients, 224 with benign and 33 with aggressive disease courses, and genotyping was performed on a MassArray iPLEX platform. Finally, a meta-analysis was performed in the two validation cohorts

    Article Snippet: TaqMan OpenArray genotyping Genotyping of selected variants in the first validation cohort was performed using an OpenArray technology (Thermo Fisher Scientific, Massachusetts, USA) and following the manufacturer’s instructions.

    Techniques: Mass Spectrometry, Sequencing, Selection, Variant Assay

    IGV visualization of human liver CYP2C19 mRNA. RNA-Seq results for human livers that were heterozygous (half-filled box) or homozygous wild type (open box). (A) rs12769205 were visualized across CYP2C19 exons 2-Exon3; and (B) rs4244285 were visualized

    Journal: Drug Metabolism and Disposition

    Article Title: The CYP2C19 Intron 2 Branch Point SNP is the Ancestral Polymorphism Contributing to the Poor Metabolizer Phenotype in Livers with CYP2C19*35 and CYP2C19*2

    doi: 10.1124/dmd.115.064428

    Figure Lengend Snippet: IGV visualization of human liver CYP2C19 mRNA. RNA-Seq results for human livers that were heterozygous (half-filled box) or homozygous wild type (open box). (A) rs12769205 were visualized across CYP2C19 exons 2-Exon3; and (B) rs4244285 were visualized

    Article Snippet: Total RNA was extracted from human livers with rs4244285 and rs12769205 genotypes using TRIzol reagent (Invitrogen, Carlsbad, CA).

    Techniques: RNA Sequencing Assay

    rs12769205 Is in LD with rs4244285 on CYP2C19*2 , but Occurs Independently on the CYP2C19*35 Allele in Some Blacks.

    Journal: Drug Metabolism and Disposition

    Article Title: The CYP2C19 Intron 2 Branch Point SNP is the Ancestral Polymorphism Contributing to the Poor Metabolizer Phenotype in Livers with CYP2C19*35 and CYP2C19*2

    doi: 10.1124/dmd.115.064428

    Figure Lengend Snippet: rs12769205 Is in LD with rs4244285 on CYP2C19*2 , but Occurs Independently on the CYP2C19*35 Allele in Some Blacks.

    Article Snippet: Total RNA was extracted from human livers with rs4244285 and rs12769205 genotypes using TRIzol reagent (Invitrogen, Carlsbad, CA).

    Techniques:

    CYP2C19 haplotype frequencies, extended haplotype homozygosity, and ancestral tree in 216 YRI chromosomes. (A) Sweep was used to determine CYP2C19 haplotypes (SNP positions rs12769205, rs4244285, rs4417205, and rs3758580) and their frequencies. The online

    Journal: Drug Metabolism and Disposition

    Article Title: The CYP2C19 Intron 2 Branch Point SNP is the Ancestral Polymorphism Contributing to the Poor Metabolizer Phenotype in Livers with CYP2C19*35 and CYP2C19*2

    doi: 10.1124/dmd.115.064428

    Figure Lengend Snippet: CYP2C19 haplotype frequencies, extended haplotype homozygosity, and ancestral tree in 216 YRI chromosomes. (A) Sweep was used to determine CYP2C19 haplotypes (SNP positions rs12769205, rs4244285, rs4417205, and rs3758580) and their frequencies. The online

    Article Snippet: Total RNA was extracted from human livers with rs4244285 and rs12769205 genotypes using TRIzol reagent (Invitrogen, Carlsbad, CA).

    Techniques:

    Effect of rs4244285 and rs12769205 on CYP2C19 splicing. (A) Eight liver samples were analyzed by PCR for (A) the CYP2C19 exon 5–40 bp deletion, caused by rs4244285, using primers in exons 4 and 6, and (B) the CYP2C19 exon 2B insertion, caused

    Journal: Drug Metabolism and Disposition

    Article Title: The CYP2C19 Intron 2 Branch Point SNP is the Ancestral Polymorphism Contributing to the Poor Metabolizer Phenotype in Livers with CYP2C19*35 and CYP2C19*2

    doi: 10.1124/dmd.115.064428

    Figure Lengend Snippet: Effect of rs4244285 and rs12769205 on CYP2C19 splicing. (A) Eight liver samples were analyzed by PCR for (A) the CYP2C19 exon 5–40 bp deletion, caused by rs4244285, using primers in exons 4 and 6, and (B) the CYP2C19 exon 2B insertion, caused

    Article Snippet: Total RNA was extracted from human livers with rs4244285 and rs12769205 genotypes using TRIzol reagent (Invitrogen, Carlsbad, CA).

    Techniques: Polymerase Chain Reaction

    Structure of alternative CYP2C19 mRNAs in livers with rs4244285 and rs12769205 genotypes. CYP2C19 was PCR amplified using exon 2 and 6 primers in liver samples with indicated genotypes and the products were analyzed on agarose gels. Arrows indicate the

    Journal: Drug Metabolism and Disposition

    Article Title: The CYP2C19 Intron 2 Branch Point SNP is the Ancestral Polymorphism Contributing to the Poor Metabolizer Phenotype in Livers with CYP2C19*35 and CYP2C19*2

    doi: 10.1124/dmd.115.064428

    Figure Lengend Snippet: Structure of alternative CYP2C19 mRNAs in livers with rs4244285 and rs12769205 genotypes. CYP2C19 was PCR amplified using exon 2 and 6 primers in liver samples with indicated genotypes and the products were analyzed on agarose gels. Arrows indicate the

    Article Snippet: Total RNA was extracted from human livers with rs4244285 and rs12769205 genotypes using TRIzol reagent (Invitrogen, Carlsbad, CA).

    Techniques: Polymerase Chain Reaction, Amplification

    CYP2C19 peptides downstream from exon 2 failed to detect residual CYP2C19 wild-type protein in CYP2C19*2 and CYP2C19*35 liver microsomes. (A) The location of the peptide probes used relative to CYP2C19 exons and to rs12769205 and rs4244285. (B) Expression

    Journal: Drug Metabolism and Disposition

    Article Title: The CYP2C19 Intron 2 Branch Point SNP is the Ancestral Polymorphism Contributing to the Poor Metabolizer Phenotype in Livers with CYP2C19*35 and CYP2C19*2

    doi: 10.1124/dmd.115.064428

    Figure Lengend Snippet: CYP2C19 peptides downstream from exon 2 failed to detect residual CYP2C19 wild-type protein in CYP2C19*2 and CYP2C19*35 liver microsomes. (A) The location of the peptide probes used relative to CYP2C19 exons and to rs12769205 and rs4244285. (B) Expression

    Article Snippet: Total RNA was extracted from human livers with rs4244285 and rs12769205 genotypes using TRIzol reagent (Invitrogen, Carlsbad, CA).

    Techniques: Expressing

    3.2. Modification of IL-28B SNP rs8099917 and rs12979860 Genotypes after LDLT

    Journal: BioMed Research International

    Article Title: Identification of IL-28B Genotype Modification in Hepatocytes after Living Donor Liver Transplantation by Laser Capture Microdissection and Pyrosequencing Analysis

    doi: 10.1155/2018/1826140

    Figure Lengend Snippet: 3.2. Modification of IL-28B SNP rs8099917 and rs12979860 Genotypes after LDLT

    Article Snippet: IL-28B SNP rs8099917 and rs12979860 genotypes were studied in liver tissues, donor's blood samples, and recipient's blood samples on POD0, POD7, and POD30, using a 7500 Fast Real-Time PCR System (Applied Biosystems, Foster City, CA, USA) with Custom TaqMan SNP Genotyping Assays (Applied Biosystems) for allele discrimination.

    Techniques: Modification

    3.2. Modification of IL-28B SNP rs8099917 and rs12979860 Genotypes after LDLT

    Journal: BioMed Research International

    Article Title: Identification of IL-28B Genotype Modification in Hepatocytes after Living Donor Liver Transplantation by Laser Capture Microdissection and Pyrosequencing Analysis

    doi: 10.1155/2018/1826140

    Figure Lengend Snippet: 3.2. Modification of IL-28B SNP rs8099917 and rs12979860 Genotypes after LDLT

    Article Snippet: IL-28B SNP rs8099917 and rs12979860 genotypes were studied in liver tissues, donor's blood samples, and recipient's blood samples on POD0, POD7, and POD30, using a 7500 Fast Real-Time PCR System (Applied Biosystems, Foster City, CA, USA) with Custom TaqMan SNP Genotyping Assays (Applied Biosystems) for allele discrimination.

    Techniques: Modification

    Genotyping and characterization of linkage disequilibrium

    Journal: Molecular Ecology Resources

    Article Title: Estimation of linkage disequilibrium and interspecific gene flow in Ficedula flycatchers by a newly developed 50k single-nucleotide polymorphism array

    doi: 10.1111/1755-0998.12270

    Figure Lengend Snippet: Genotyping and characterization of linkage disequilibrium

    Article Snippet: Genotyping and characterization of linkage disequilibrium Genotyping was performed on an Illumina iScan instrument at the SNP & Seq Technology Platform at Uppsala University ( http://www.molmed.medsci.uu.se/SNP+SEQ+Technology+Platform/ ).

    Techniques:

    Genomic structure and pair-wise marker LD in ADIPOR1 ( a ) and ADIPOR2 ( b ). The location of SNPs identified in this study and/or genotyped is represented along the gene ( bold type : SNPs selected for genotyping, and failed or monomorphic SNPs ). Exons are represented in boxes ( black for coding and open for untranslated ). Introns and flanking sequences appear as lines . The pair-wise marker LD measured by r 2 statistics is shown below the genomic structures and indicated by the shade of grey blocks ( white to black ) and the r 2 value. a , b , isoforms a and b in ADIPOR2

    Journal: Diabetologia

    Article Title: Adiponectin receptor genes: mutation screening in syndromes of insulin resistance and association studies for type 2 diabetes and metabolic traits in UK populations

    doi: 10.1007/s00125-006-0534-7

    Figure Lengend Snippet: Genomic structure and pair-wise marker LD in ADIPOR1 ( a ) and ADIPOR2 ( b ). The location of SNPs identified in this study and/or genotyped is represented along the gene ( bold type : SNPs selected for genotyping, and failed or monomorphic SNPs ). Exons are represented in boxes ( black for coding and open for untranslated ). Introns and flanking sequences appear as lines . The pair-wise marker LD measured by r 2 statistics is shown below the genomic structures and indicated by the shade of grey blocks ( white to black ) and the r 2 value. a , b , isoforms a and b in ADIPOR2

    Article Snippet: Genotyping of samples was performed in 384-well plates at the Wellcome Trust Sanger Institute, Cambridge, using an adaptation of the homogenous MassExtend protocol for the MassArray system (Sequenom, San Diego, CA, USA) [ ].

    Techniques: Marker

    SNP calling pattern, genome density and polymorphism of SNPs in ‘Reference Set’ using Axiom_ Arachis SNP array. This figure shows ( a , b ) monomorphic SNPs identified in the genotyping data, ( c ) polymorphic SNPs without heterozygosity i.e., homozygous SNPs, ( d ) polymorphic SNPs with heterozygosity, ( e ) genome-wide distribution of SNPs, and ( f ) pseudomolecule-wise distribution of SNPs on array and polymorphic SNPs in the ‘Reference Set’.

    Journal: Scientific Reports

    Article Title: Development and Evaluation of a High Density Genotyping ‘Axiom_Arachis’ Array with 58 K SNPs for Accelerating Genetics and Breeding in Groundnut

    doi: 10.1038/srep40577

    Figure Lengend Snippet: SNP calling pattern, genome density and polymorphism of SNPs in ‘Reference Set’ using Axiom_ Arachis SNP array. This figure shows ( a , b ) monomorphic SNPs identified in the genotyping data, ( c ) polymorphic SNPs without heterozygosity i.e., homozygous SNPs, ( d ) polymorphic SNPs with heterozygosity, ( e ) genome-wide distribution of SNPs, and ( f ) pseudomolecule-wise distribution of SNPs on array and polymorphic SNPs in the ‘Reference Set’.

    Article Snippet: SNP allele calling and data analysis Allele calling was done using Axiom™ Analysis Suite version 1.0 using its three workflows i.e., Best Practices, Sample QC, Genotyping and Summary Only ( http://media.affymetrix.com/support/downloads/manuals/axiom_analysis_suite_user_guide.pdf ).

    Techniques: Genome Wide

    FOXC2 is able to functionally substitute for FOXC1 during lymphatic valve development. ( a ) Schematic representation of the targeting vector and targeted allele. The entire protein coding region of Foxc1 is replaced with that of Foxc2 . ACN, self-excision cassette including Cre driven by the testis-specific promoter. ( b ) Southern blot analysis to detect double-resistant ES cell colonies using 5’ and 3’ probes. ( c ) PCR genotyping of F1 heterozygotes to detect the Foxc1 c2 allele. ( d, e ) Representative images of lymphatic valves in mesenteric collecting vessels immunostained with antibodies targeted to FOXC1 and VEGFR-3 from P12 P6 Foxc1 +/+ ( d ) and Foxc1 c2/c2 ( e ) mice. Scale bars are 25 µm. (f – h) Representative images of lymphatic valves in mesenteric collecting vessels immunostained with antibodies targeted to FOXC1, FOXC2, and VE-Cadherin from P6 Foxc1 +/+ ( f ) , Foxc1 c2/+ ( g ), and Foxc1 c2/c2 mice ( h ). Scale bars are 50 μm. (i – k) Representative images of the mesenteric vasculature immunostained with antibodies targeted to PROX1 and CD31 in P6 Foxc1 +/+ ( i ) , Foxc1 c2/+ ( j ), and Foxc1 c2/c2 mice ( k ). Scale bars are 200 μm. (l – n) Representative images of P6 mesenteric vasculature from P6 Foxc1 +/+ ( l ) , Foxc1 c2/+ ( m ), and Foxc1 c2/c2 mice ( n ) immunostained with antibodies targeted to FOXC1, FOXC2, and VE-Cadherin show gradual loss of FOXC1 expression in the blood and lymphatic vasculature and smooth muscle cells and conversely the increase of FOXC2 expression in blood vasculature and smooth muscle. Scale bars are 200 μm. ( o ) Quantification of total lymphatic valve number in lymphatic collecting vessels of P6 Foxc1 +/+ , Foxc1 c2/+ , and Foxc1 c2/c2 individuals. N = 6 for Foxc1 +/+ , N = 8 for Foxc1 c2/+ , and N = 8 for Foxc1 c2/c2 individuals. ( p ) Percentage of mature and immature lymphatic valves normalized to total valves counted in P6 Foxc1 +/+ , Foxc1 c2/+ , and Foxc1 c2/c2 individuals. Data are presented as mean (± SD) and analyzed using Student’s t-test. NS denotes no significance.

    Journal: eLife

    Article Title: Shear stimulation of FOXC1 and FOXC2 differentially regulates cytoskeletal activity during lymphatic valve maturation

    doi: 10.7554/eLife.53814

    Figure Lengend Snippet: FOXC2 is able to functionally substitute for FOXC1 during lymphatic valve development. ( a ) Schematic representation of the targeting vector and targeted allele. The entire protein coding region of Foxc1 is replaced with that of Foxc2 . ACN, self-excision cassette including Cre driven by the testis-specific promoter. ( b ) Southern blot analysis to detect double-resistant ES cell colonies using 5’ and 3’ probes. ( c ) PCR genotyping of F1 heterozygotes to detect the Foxc1 c2 allele. ( d, e ) Representative images of lymphatic valves in mesenteric collecting vessels immunostained with antibodies targeted to FOXC1 and VEGFR-3 from P12 P6 Foxc1 +/+ ( d ) and Foxc1 c2/c2 ( e ) mice. Scale bars are 25 µm. (f – h) Representative images of lymphatic valves in mesenteric collecting vessels immunostained with antibodies targeted to FOXC1, FOXC2, and VE-Cadherin from P6 Foxc1 +/+ ( f ) , Foxc1 c2/+ ( g ), and Foxc1 c2/c2 mice ( h ). Scale bars are 50 μm. (i – k) Representative images of the mesenteric vasculature immunostained with antibodies targeted to PROX1 and CD31 in P6 Foxc1 +/+ ( i ) , Foxc1 c2/+ ( j ), and Foxc1 c2/c2 mice ( k ). Scale bars are 200 μm. (l – n) Representative images of P6 mesenteric vasculature from P6 Foxc1 +/+ ( l ) , Foxc1 c2/+ ( m ), and Foxc1 c2/c2 mice ( n ) immunostained with antibodies targeted to FOXC1, FOXC2, and VE-Cadherin show gradual loss of FOXC1 expression in the blood and lymphatic vasculature and smooth muscle cells and conversely the increase of FOXC2 expression in blood vasculature and smooth muscle. Scale bars are 200 μm. ( o ) Quantification of total lymphatic valve number in lymphatic collecting vessels of P6 Foxc1 +/+ , Foxc1 c2/+ , and Foxc1 c2/c2 individuals. N = 6 for Foxc1 +/+ , N = 8 for Foxc1 c2/+ , and N = 8 for Foxc1 c2/c2 individuals. ( p ) Percentage of mature and immature lymphatic valves normalized to total valves counted in P6 Foxc1 +/+ , Foxc1 c2/+ , and Foxc1 c2/c2 individuals. Data are presented as mean (± SD) and analyzed using Student’s t-test. NS denotes no significance.

    Article Snippet: Genotyping of mice for use in analysis was performed by Transnetyx Inc (Cordova, TN) using real-time PCR.

    Techniques: Plasmid Preparation, Southern Blot, Polymerase Chain Reaction, Mouse Assay, Expressing

    Characterisation of MCK-tTA-hYAP1 S127A mouse model. A) Schematic outline of the inducible ‘Tet-Off’ transgenic system. B) Genotyping of MCK-tTA and TRE-hYAP1 S127A alleles in single and double transgenic mice. C) Yap protein levels in skeletal muscles of transgenic mice of indicated genotypes following 25 days with (+) or without (−) doxycycline (dox). Tibialis anterior (TA), gastrocnemius (Gas) and extensor digitorum longus (EDL). D) Muscle specific expression of hYAP mRNA in MCK-tTA-hYAP1 S127A mice 25 days after doxycycline withdrawal. E) Time course of Yap protein expression in tibialis anterior of MCK-tTA-hYAP1 S127A mice following doxycycline removal.

    Journal: PLoS ONE

    Article Title: Constitutive Expression of Yes-Associated Protein (Yap) in Adult Skeletal Muscle Fibres Induces Muscle Atrophy and Myopathy

    doi: 10.1371/journal.pone.0059622

    Figure Lengend Snippet: Characterisation of MCK-tTA-hYAP1 S127A mouse model. A) Schematic outline of the inducible ‘Tet-Off’ transgenic system. B) Genotyping of MCK-tTA and TRE-hYAP1 S127A alleles in single and double transgenic mice. C) Yap protein levels in skeletal muscles of transgenic mice of indicated genotypes following 25 days with (+) or without (−) doxycycline (dox). Tibialis anterior (TA), gastrocnemius (Gas) and extensor digitorum longus (EDL). D) Muscle specific expression of hYAP mRNA in MCK-tTA-hYAP1 S127A mice 25 days after doxycycline withdrawal. E) Time course of Yap protein expression in tibialis anterior of MCK-tTA-hYAP1 S127A mice following doxycycline removal.

    Article Snippet: PCR primers for genotyping, end-point PCR primers for hYAP and Gapdh mRNA and qRT-PCR primers and probes (Roche Universal Probe Library). (DOCX) Click here for additional data file.

    Techniques: Transgenic Assay, Mouse Assay, Expressing