genomic dna Thermo Fisher Search Results


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  • 99
    Zymo Research quick gdna miniprep kit
    Quick Gdna Miniprep Kit, supplied by Zymo Research, used in various techniques. Bioz Stars score: 99/100, based on 825 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    Average 99 stars, based on 825 article reviews
    Price from $9.99 to $1999.99
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    80
    Thermo Fisher easy dna genomic dna gdna purification kit
    Easy Dna Genomic Dna Gdna Purification Kit, supplied by Thermo Fisher, used in various techniques. Bioz Stars score: 80/100, based on 8 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    Average 80 stars, based on 8 article reviews
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    94
    Thermo Fisher gdna eraser
    Gdna Eraser, supplied by Thermo Fisher, used in various techniques. Bioz Stars score: 94/100, based on 113 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    Average 94 stars, based on 113 article reviews
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    92
    Thermo Fisher gdna remover
    Gdna Remover, supplied by Thermo Fisher, used in various techniques. Bioz Stars score: 92/100, based on 1520 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    Average 92 stars, based on 1520 article reviews
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    90
    Thermo Fisher transscript one step genomic dna gdna removal
    Transscript One Step Genomic Dna Gdna Removal, supplied by Thermo Fisher, used in various techniques. Bioz Stars score: 90/100, based on 10 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    Average 90 stars, based on 10 article reviews
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    81
    Thermo Fisher gdna kit
    Gdna Kit, supplied by Thermo Fisher, used in various techniques. Bioz Stars score: 81/100, based on 9 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    Average 81 stars, based on 9 article reviews
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    gdna kit - by Bioz Stars, 2020-01
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    82
    Thermo Fisher chargeswitch gdna kit
    Chargeswitch Gdna Kit, supplied by Thermo Fisher, used in various techniques. Bioz Stars score: 82/100, based on 36 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    Average 82 stars, based on 36 article reviews
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    94
    Thermo Fisher genomic dna gdna
    Genomic Dna Gdna, supplied by Thermo Fisher, used in various techniques. Bioz Stars score: 94/100, based on 817 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    Average 94 stars, based on 817 article reviews
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    85
    Thermo Fisher chargeswitch gdna
    Chargeswitch Gdna, supplied by Thermo Fisher, used in various techniques. Bioz Stars score: 85/100, based on 20 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    Average 85 stars, based on 20 article reviews
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    88
    Thermo Fisher mouse gdna
    Mouse Gdna, supplied by Thermo Fisher, used in various techniques. Bioz Stars score: 88/100, based on 50 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    Average 88 stars, based on 50 article reviews
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    80
    Thermo Fisher gdna remover kits
    Gdna Remover Kits, supplied by Thermo Fisher, used in various techniques. Bioz Stars score: 80/100, based on 8 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    Average 80 stars, based on 8 article reviews
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    91
    Thermo Fisher chargeswitch genomic dna gdna mini bacteria kit
    Chargeswitch Genomic Dna Gdna Mini Bacteria Kit, supplied by Thermo Fisher, used in various techniques. Bioz Stars score: 91/100, based on 41 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    Average 91 stars, based on 41 article reviews
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    94
    Thermo Fisher human gdna
    Gel and quantitative polymerase chain reaction (PCR) assays utilizing both existing and new PCR primers. A , Gel picture showing PCR products for the genomic DNA <t>(gDNA)</t> of a Solomon Islands (SI) sample WP0022.7liq, and a non-SI sample, Treponema <t>pallidum</t> subsp pertenue Gauthier, with the use of the generic forward and reverse primers as indicated. The reverse primer of the Centers for Disease Control and Prevention (CDC) 2015 real-time (RT) PCR assay is labeled reverse primer in Figure 2 . Forward primer (2015 CDC RT-PCR): 5ʹ-CGGCCACCAACTTGGGATTGAC-3ʹ. Reverse primer (2015 CDC RT-PCR): 5ʹ-CGTACACCGAACCTTTGTCTT-3ʹ. Reverse primer (modified PCR-1): 5ʹ- GTGCGGTGAGCCCGGCGTT-3ʹ. Reverse primer (modified PCR-2): 5ʹ-GTGAGCCCGGCGTT-3ʹ. B , Quantitative PCR amplification curves of new PCR products obtained for the gDNA of the non-SI sample, an SI sample, and both samples (all as in A ) combined by using specific reverse primers. Forward primer (2015 CDC RT-PCR): 5ʹ-CGGCCACCAACTTGGGATTGAC-3ʹ. Probe: 5ʹ-FAM-GCTGCAAGGAGAAGTCCTGCTGC-TAMRA-3ʹ. Reverse primer (2015 CDC real-time PCR): 5ʹ-CGTACACCGAACCTTTGTCTT-3ʹ. Reverse primer (modified PCR-2): 5ʹ-GTGAGCCCGGCGTT-3ʹ. Abbreviations: ∆Rn, normalized reporter signal; CDC, Centers for Disease Control and Prevention; DDW, double distilled water; RT-PCR, real-time polymerase chain reaction; SI, Solomon Islands.
    Human Gdna, supplied by Thermo Fisher, used in various techniques. Bioz Stars score: 94/100, based on 71 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    Average 94 stars, based on 71 article reviews
    Price from $9.99 to $1999.99
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    94/100 stars
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    81
    Thermo Fisher genecatcher gdna kit
    Gel and quantitative polymerase chain reaction (PCR) assays utilizing both existing and new PCR primers. A , Gel picture showing PCR products for the genomic DNA <t>(gDNA)</t> of a Solomon Islands (SI) sample WP0022.7liq, and a non-SI sample, Treponema <t>pallidum</t> subsp pertenue Gauthier, with the use of the generic forward and reverse primers as indicated. The reverse primer of the Centers for Disease Control and Prevention (CDC) 2015 real-time (RT) PCR assay is labeled reverse primer in Figure 2 . Forward primer (2015 CDC RT-PCR): 5ʹ-CGGCCACCAACTTGGGATTGAC-3ʹ. Reverse primer (2015 CDC RT-PCR): 5ʹ-CGTACACCGAACCTTTGTCTT-3ʹ. Reverse primer (modified PCR-1): 5ʹ- GTGCGGTGAGCCCGGCGTT-3ʹ. Reverse primer (modified PCR-2): 5ʹ-GTGAGCCCGGCGTT-3ʹ. B , Quantitative PCR amplification curves of new PCR products obtained for the gDNA of the non-SI sample, an SI sample, and both samples (all as in A ) combined by using specific reverse primers. Forward primer (2015 CDC RT-PCR): 5ʹ-CGGCCACCAACTTGGGATTGAC-3ʹ. Probe: 5ʹ-FAM-GCTGCAAGGAGAAGTCCTGCTGC-TAMRA-3ʹ. Reverse primer (2015 CDC real-time PCR): 5ʹ-CGTACACCGAACCTTTGTCTT-3ʹ. Reverse primer (modified PCR-2): 5ʹ-GTGAGCCCGGCGTT-3ʹ. Abbreviations: ∆Rn, normalized reporter signal; CDC, Centers for Disease Control and Prevention; DDW, double distilled water; RT-PCR, real-time polymerase chain reaction; SI, Solomon Islands.
    Genecatcher Gdna Kit, supplied by Thermo Fisher, used in various techniques. Bioz Stars score: 81/100, based on 7 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    Average 81 stars, based on 7 article reviews
    Price from $9.99 to $1999.99
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    78
    Thermo Fisher iprep purelink gdna kit
    Gel and quantitative polymerase chain reaction (PCR) assays utilizing both existing and new PCR primers. A , Gel picture showing PCR products for the genomic DNA <t>(gDNA)</t> of a Solomon Islands (SI) sample WP0022.7liq, and a non-SI sample, Treponema <t>pallidum</t> subsp pertenue Gauthier, with the use of the generic forward and reverse primers as indicated. The reverse primer of the Centers for Disease Control and Prevention (CDC) 2015 real-time (RT) PCR assay is labeled reverse primer in Figure 2 . Forward primer (2015 CDC RT-PCR): 5ʹ-CGGCCACCAACTTGGGATTGAC-3ʹ. Reverse primer (2015 CDC RT-PCR): 5ʹ-CGTACACCGAACCTTTGTCTT-3ʹ. Reverse primer (modified PCR-1): 5ʹ- GTGCGGTGAGCCCGGCGTT-3ʹ. Reverse primer (modified PCR-2): 5ʹ-GTGAGCCCGGCGTT-3ʹ. B , Quantitative PCR amplification curves of new PCR products obtained for the gDNA of the non-SI sample, an SI sample, and both samples (all as in A ) combined by using specific reverse primers. Forward primer (2015 CDC RT-PCR): 5ʹ-CGGCCACCAACTTGGGATTGAC-3ʹ. Probe: 5ʹ-FAM-GCTGCAAGGAGAAGTCCTGCTGC-TAMRA-3ʹ. Reverse primer (2015 CDC real-time PCR): 5ʹ-CGTACACCGAACCTTTGTCTT-3ʹ. Reverse primer (modified PCR-2): 5ʹ-GTGAGCCCGGCGTT-3ʹ. Abbreviations: ∆Rn, normalized reporter signal; CDC, Centers for Disease Control and Prevention; DDW, double distilled water; RT-PCR, real-time polymerase chain reaction; SI, Solomon Islands.
    Iprep Purelink Gdna Kit, supplied by Thermo Fisher, used in various techniques. Bioz Stars score: 78/100, based on 4 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    Average 78 stars, based on 4 article reviews
    Price from $9.99 to $1999.99
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    78
    Thermo Fisher genomic dna plant chargeswitch kit
    Gel and quantitative polymerase chain reaction (PCR) assays utilizing both existing and new PCR primers. A , Gel picture showing PCR products for the genomic DNA <t>(gDNA)</t> of a Solomon Islands (SI) sample WP0022.7liq, and a non-SI sample, Treponema <t>pallidum</t> subsp pertenue Gauthier, with the use of the generic forward and reverse primers as indicated. The reverse primer of the Centers for Disease Control and Prevention (CDC) 2015 real-time (RT) PCR assay is labeled reverse primer in Figure 2 . Forward primer (2015 CDC RT-PCR): 5ʹ-CGGCCACCAACTTGGGATTGAC-3ʹ. Reverse primer (2015 CDC RT-PCR): 5ʹ-CGTACACCGAACCTTTGTCTT-3ʹ. Reverse primer (modified PCR-1): 5ʹ- GTGCGGTGAGCCCGGCGTT-3ʹ. Reverse primer (modified PCR-2): 5ʹ-GTGAGCCCGGCGTT-3ʹ. B , Quantitative PCR amplification curves of new PCR products obtained for the gDNA of the non-SI sample, an SI sample, and both samples (all as in A ) combined by using specific reverse primers. Forward primer (2015 CDC RT-PCR): 5ʹ-CGGCCACCAACTTGGGATTGAC-3ʹ. Probe: 5ʹ-FAM-GCTGCAAGGAGAAGTCCTGCTGC-TAMRA-3ʹ. Reverse primer (2015 CDC real-time PCR): 5ʹ-CGTACACCGAACCTTTGTCTT-3ʹ. Reverse primer (modified PCR-2): 5ʹ-GTGAGCCCGGCGTT-3ʹ. Abbreviations: ∆Rn, normalized reporter signal; CDC, Centers for Disease Control and Prevention; DDW, double distilled water; RT-PCR, real-time polymerase chain reaction; SI, Solomon Islands.
    Genomic Dna Plant Chargeswitch Kit, supplied by Thermo Fisher, used in various techniques. Bioz Stars score: 78/100, based on 7 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/genomic dna plant chargeswitch kit/product/Thermo Fisher
    Average 78 stars, based on 7 article reviews
    Price from $9.99 to $1999.99
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    78/100 stars
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    78
    Thermo Fisher purelink genomic dna gdna
    Gel and quantitative polymerase chain reaction (PCR) assays utilizing both existing and new PCR primers. A , Gel picture showing PCR products for the genomic DNA <t>(gDNA)</t> of a Solomon Islands (SI) sample WP0022.7liq, and a non-SI sample, Treponema <t>pallidum</t> subsp pertenue Gauthier, with the use of the generic forward and reverse primers as indicated. The reverse primer of the Centers for Disease Control and Prevention (CDC) 2015 real-time (RT) PCR assay is labeled reverse primer in Figure 2 . Forward primer (2015 CDC RT-PCR): 5ʹ-CGGCCACCAACTTGGGATTGAC-3ʹ. Reverse primer (2015 CDC RT-PCR): 5ʹ-CGTACACCGAACCTTTGTCTT-3ʹ. Reverse primer (modified PCR-1): 5ʹ- GTGCGGTGAGCCCGGCGTT-3ʹ. Reverse primer (modified PCR-2): 5ʹ-GTGAGCCCGGCGTT-3ʹ. B , Quantitative PCR amplification curves of new PCR products obtained for the gDNA of the non-SI sample, an SI sample, and both samples (all as in A ) combined by using specific reverse primers. Forward primer (2015 CDC RT-PCR): 5ʹ-CGGCCACCAACTTGGGATTGAC-3ʹ. Probe: 5ʹ-FAM-GCTGCAAGGAGAAGTCCTGCTGC-TAMRA-3ʹ. Reverse primer (2015 CDC real-time PCR): 5ʹ-CGTACACCGAACCTTTGTCTT-3ʹ. Reverse primer (modified PCR-2): 5ʹ-GTGAGCCCGGCGTT-3ʹ. Abbreviations: ∆Rn, normalized reporter signal; CDC, Centers for Disease Control and Prevention; DDW, double distilled water; RT-PCR, real-time polymerase chain reaction; SI, Solomon Islands.
    Purelink Genomic Dna Gdna, supplied by Thermo Fisher, used in various techniques. Bioz Stars score: 78/100, based on 10 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/purelink genomic dna gdna/product/Thermo Fisher
    Average 78 stars, based on 10 article reviews
    Price from $9.99 to $1999.99
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    78/100 stars
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    70
    Thermo Fisher genejet gdna purification kit
    Gel and quantitative polymerase chain reaction (PCR) assays utilizing both existing and new PCR primers. A , Gel picture showing PCR products for the genomic DNA <t>(gDNA)</t> of a Solomon Islands (SI) sample WP0022.7liq, and a non-SI sample, Treponema <t>pallidum</t> subsp pertenue Gauthier, with the use of the generic forward and reverse primers as indicated. The reverse primer of the Centers for Disease Control and Prevention (CDC) 2015 real-time (RT) PCR assay is labeled reverse primer in Figure 2 . Forward primer (2015 CDC RT-PCR): 5ʹ-CGGCCACCAACTTGGGATTGAC-3ʹ. Reverse primer (2015 CDC RT-PCR): 5ʹ-CGTACACCGAACCTTTGTCTT-3ʹ. Reverse primer (modified PCR-1): 5ʹ- GTGCGGTGAGCCCGGCGTT-3ʹ. Reverse primer (modified PCR-2): 5ʹ-GTGAGCCCGGCGTT-3ʹ. B , Quantitative PCR amplification curves of new PCR products obtained for the gDNA of the non-SI sample, an SI sample, and both samples (all as in A ) combined by using specific reverse primers. Forward primer (2015 CDC RT-PCR): 5ʹ-CGGCCACCAACTTGGGATTGAC-3ʹ. Probe: 5ʹ-FAM-GCTGCAAGGAGAAGTCCTGCTGC-TAMRA-3ʹ. Reverse primer (2015 CDC real-time PCR): 5ʹ-CGTACACCGAACCTTTGTCTT-3ʹ. Reverse primer (modified PCR-2): 5ʹ-GTGAGCCCGGCGTT-3ʹ. Abbreviations: ∆Rn, normalized reporter signal; CDC, Centers for Disease Control and Prevention; DDW, double distilled water; RT-PCR, real-time polymerase chain reaction; SI, Solomon Islands.
    Genejet Gdna Purification Kit, supplied by Thermo Fisher, used in various techniques. Bioz Stars score: 70/100, based on 121 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/genejet gdna purification kit/product/Thermo Fisher
    Average 70 stars, based on 121 article reviews
    Price from $9.99 to $1999.99
    genejet gdna purification kit - by Bioz Stars, 2020-01
    70/100 stars
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    70
    Thermo Fisher chargeswitch gdna extraction kit
    Gel and quantitative polymerase chain reaction (PCR) assays utilizing both existing and new PCR primers. A , Gel picture showing PCR products for the genomic DNA <t>(gDNA)</t> of a Solomon Islands (SI) sample WP0022.7liq, and a non-SI sample, Treponema <t>pallidum</t> subsp pertenue Gauthier, with the use of the generic forward and reverse primers as indicated. The reverse primer of the Centers for Disease Control and Prevention (CDC) 2015 real-time (RT) PCR assay is labeled reverse primer in Figure 2 . Forward primer (2015 CDC RT-PCR): 5ʹ-CGGCCACCAACTTGGGATTGAC-3ʹ. Reverse primer (2015 CDC RT-PCR): 5ʹ-CGTACACCGAACCTTTGTCTT-3ʹ. Reverse primer (modified PCR-1): 5ʹ- GTGCGGTGAGCCCGGCGTT-3ʹ. Reverse primer (modified PCR-2): 5ʹ-GTGAGCCCGGCGTT-3ʹ. B , Quantitative PCR amplification curves of new PCR products obtained for the gDNA of the non-SI sample, an SI sample, and both samples (all as in A ) combined by using specific reverse primers. Forward primer (2015 CDC RT-PCR): 5ʹ-CGGCCACCAACTTGGGATTGAC-3ʹ. Probe: 5ʹ-FAM-GCTGCAAGGAGAAGTCCTGCTGC-TAMRA-3ʹ. Reverse primer (2015 CDC real-time PCR): 5ʹ-CGTACACCGAACCTTTGTCTT-3ʹ. Reverse primer (modified PCR-2): 5ʹ-GTGAGCCCGGCGTT-3ʹ. Abbreviations: ∆Rn, normalized reporter signal; CDC, Centers for Disease Control and Prevention; DDW, double distilled water; RT-PCR, real-time polymerase chain reaction; SI, Solomon Islands.
    Chargeswitch Gdna Extraction Kit, supplied by Thermo Fisher, used in various techniques. Bioz Stars score: 70/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/chargeswitch gdna extraction kit/product/Thermo Fisher
    Average 70 stars, based on 1 article reviews
    Price from $9.99 to $1999.99
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    70/100 stars
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    78
    Thermo Fisher chargeswitch gdna mini kit
    Gel and quantitative polymerase chain reaction (PCR) assays utilizing both existing and new PCR primers. A , Gel picture showing PCR products for the genomic DNA <t>(gDNA)</t> of a Solomon Islands (SI) sample WP0022.7liq, and a non-SI sample, Treponema <t>pallidum</t> subsp pertenue Gauthier, with the use of the generic forward and reverse primers as indicated. The reverse primer of the Centers for Disease Control and Prevention (CDC) 2015 real-time (RT) PCR assay is labeled reverse primer in Figure 2 . Forward primer (2015 CDC RT-PCR): 5ʹ-CGGCCACCAACTTGGGATTGAC-3ʹ. Reverse primer (2015 CDC RT-PCR): 5ʹ-CGTACACCGAACCTTTGTCTT-3ʹ. Reverse primer (modified PCR-1): 5ʹ- GTGCGGTGAGCCCGGCGTT-3ʹ. Reverse primer (modified PCR-2): 5ʹ-GTGAGCCCGGCGTT-3ʹ. B , Quantitative PCR amplification curves of new PCR products obtained for the gDNA of the non-SI sample, an SI sample, and both samples (all as in A ) combined by using specific reverse primers. Forward primer (2015 CDC RT-PCR): 5ʹ-CGGCCACCAACTTGGGATTGAC-3ʹ. Probe: 5ʹ-FAM-GCTGCAAGGAGAAGTCCTGCTGC-TAMRA-3ʹ. Reverse primer (2015 CDC real-time PCR): 5ʹ-CGTACACCGAACCTTTGTCTT-3ʹ. Reverse primer (modified PCR-2): 5ʹ-GTGAGCCCGGCGTT-3ʹ. Abbreviations: ∆Rn, normalized reporter signal; CDC, Centers for Disease Control and Prevention; DDW, double distilled water; RT-PCR, real-time polymerase chain reaction; SI, Solomon Islands.
    Chargeswitch Gdna Mini Kit, supplied by Thermo Fisher, used in various techniques. Bioz Stars score: 78/100, based on 6 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/chargeswitch gdna mini kit/product/Thermo Fisher
    Average 78 stars, based on 6 article reviews
    Price from $9.99 to $1999.99
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    78/100 stars
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    79
    Thermo Fisher genecatcher gdna automated blood kit
    Gel and quantitative polymerase chain reaction (PCR) assays utilizing both existing and new PCR primers. A , Gel picture showing PCR products for the genomic DNA <t>(gDNA)</t> of a Solomon Islands (SI) sample WP0022.7liq, and a non-SI sample, Treponema <t>pallidum</t> subsp pertenue Gauthier, with the use of the generic forward and reverse primers as indicated. The reverse primer of the Centers for Disease Control and Prevention (CDC) 2015 real-time (RT) PCR assay is labeled reverse primer in Figure 2 . Forward primer (2015 CDC RT-PCR): 5ʹ-CGGCCACCAACTTGGGATTGAC-3ʹ. Reverse primer (2015 CDC RT-PCR): 5ʹ-CGTACACCGAACCTTTGTCTT-3ʹ. Reverse primer (modified PCR-1): 5ʹ- GTGCGGTGAGCCCGGCGTT-3ʹ. Reverse primer (modified PCR-2): 5ʹ-GTGAGCCCGGCGTT-3ʹ. B , Quantitative PCR amplification curves of new PCR products obtained for the gDNA of the non-SI sample, an SI sample, and both samples (all as in A ) combined by using specific reverse primers. Forward primer (2015 CDC RT-PCR): 5ʹ-CGGCCACCAACTTGGGATTGAC-3ʹ. Probe: 5ʹ-FAM-GCTGCAAGGAGAAGTCCTGCTGC-TAMRA-3ʹ. Reverse primer (2015 CDC real-time PCR): 5ʹ-CGTACACCGAACCTTTGTCTT-3ʹ. Reverse primer (modified PCR-2): 5ʹ-GTGAGCCCGGCGTT-3ʹ. Abbreviations: ∆Rn, normalized reporter signal; CDC, Centers for Disease Control and Prevention; DDW, double distilled water; RT-PCR, real-time polymerase chain reaction; SI, Solomon Islands.
    Genecatcher Gdna Automated Blood Kit, supplied by Thermo Fisher, used in various techniques. Bioz Stars score: 79/100, based on 4 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    Average 79 stars, based on 4 article reviews
    Price from $9.99 to $1999.99
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    79/100 stars
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    80
    Thermo Fisher genejet genomic dna gdna isolation kit
    Gel and quantitative polymerase chain reaction (PCR) assays utilizing both existing and new PCR primers. A , Gel picture showing PCR products for the genomic DNA <t>(gDNA)</t> of a Solomon Islands (SI) sample WP0022.7liq, and a non-SI sample, Treponema <t>pallidum</t> subsp pertenue Gauthier, with the use of the generic forward and reverse primers as indicated. The reverse primer of the Centers for Disease Control and Prevention (CDC) 2015 real-time (RT) PCR assay is labeled reverse primer in Figure 2 . Forward primer (2015 CDC RT-PCR): 5ʹ-CGGCCACCAACTTGGGATTGAC-3ʹ. Reverse primer (2015 CDC RT-PCR): 5ʹ-CGTACACCGAACCTTTGTCTT-3ʹ. Reverse primer (modified PCR-1): 5ʹ- GTGCGGTGAGCCCGGCGTT-3ʹ. Reverse primer (modified PCR-2): 5ʹ-GTGAGCCCGGCGTT-3ʹ. B , Quantitative PCR amplification curves of new PCR products obtained for the gDNA of the non-SI sample, an SI sample, and both samples (all as in A ) combined by using specific reverse primers. Forward primer (2015 CDC RT-PCR): 5ʹ-CGGCCACCAACTTGGGATTGAC-3ʹ. Probe: 5ʹ-FAM-GCTGCAAGGAGAAGTCCTGCTGC-TAMRA-3ʹ. Reverse primer (2015 CDC real-time PCR): 5ʹ-CGTACACCGAACCTTTGTCTT-3ʹ. Reverse primer (modified PCR-2): 5ʹ-GTGAGCCCGGCGTT-3ʹ. Abbreviations: ∆Rn, normalized reporter signal; CDC, Centers for Disease Control and Prevention; DDW, double distilled water; RT-PCR, real-time polymerase chain reaction; SI, Solomon Islands.
    Genejet Genomic Dna Gdna Isolation Kit, supplied by Thermo Fisher, used in various techniques. Bioz Stars score: 80/100, based on 5 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/genejet genomic dna gdna isolation kit/product/Thermo Fisher
    Average 80 stars, based on 5 article reviews
    Price from $9.99 to $1999.99
    genejet genomic dna gdna isolation kit - by Bioz Stars, 2020-01
    80/100 stars
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    Thermo Fisher jurkat cell line gdna
    Gel and quantitative polymerase chain reaction (PCR) assays utilizing both existing and new PCR primers. A , Gel picture showing PCR products for the genomic DNA <t>(gDNA)</t> of a Solomon Islands (SI) sample WP0022.7liq, and a non-SI sample, Treponema <t>pallidum</t> subsp pertenue Gauthier, with the use of the generic forward and reverse primers as indicated. The reverse primer of the Centers for Disease Control and Prevention (CDC) 2015 real-time (RT) PCR assay is labeled reverse primer in Figure 2 . Forward primer (2015 CDC RT-PCR): 5ʹ-CGGCCACCAACTTGGGATTGAC-3ʹ. Reverse primer (2015 CDC RT-PCR): 5ʹ-CGTACACCGAACCTTTGTCTT-3ʹ. Reverse primer (modified PCR-1): 5ʹ- GTGCGGTGAGCCCGGCGTT-3ʹ. Reverse primer (modified PCR-2): 5ʹ-GTGAGCCCGGCGTT-3ʹ. B , Quantitative PCR amplification curves of new PCR products obtained for the gDNA of the non-SI sample, an SI sample, and both samples (all as in A ) combined by using specific reverse primers. Forward primer (2015 CDC RT-PCR): 5ʹ-CGGCCACCAACTTGGGATTGAC-3ʹ. Probe: 5ʹ-FAM-GCTGCAAGGAGAAGTCCTGCTGC-TAMRA-3ʹ. Reverse primer (2015 CDC real-time PCR): 5ʹ-CGTACACCGAACCTTTGTCTT-3ʹ. Reverse primer (modified PCR-2): 5ʹ-GTGAGCCCGGCGTT-3ʹ. Abbreviations: ∆Rn, normalized reporter signal; CDC, Centers for Disease Control and Prevention; DDW, double distilled water; RT-PCR, real-time polymerase chain reaction; SI, Solomon Islands.
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    Gel and quantitative polymerase chain reaction (PCR) assays utilizing both existing and new PCR primers. A , Gel picture showing PCR products for the genomic DNA <t>(gDNA)</t> of a Solomon Islands (SI) sample WP0022.7liq, and a non-SI sample, Treponema <t>pallidum</t> subsp pertenue Gauthier, with the use of the generic forward and reverse primers as indicated. The reverse primer of the Centers for Disease Control and Prevention (CDC) 2015 real-time (RT) PCR assay is labeled reverse primer in Figure 2 . Forward primer (2015 CDC RT-PCR): 5ʹ-CGGCCACCAACTTGGGATTGAC-3ʹ. Reverse primer (2015 CDC RT-PCR): 5ʹ-CGTACACCGAACCTTTGTCTT-3ʹ. Reverse primer (modified PCR-1): 5ʹ- GTGCGGTGAGCCCGGCGTT-3ʹ. Reverse primer (modified PCR-2): 5ʹ-GTGAGCCCGGCGTT-3ʹ. B , Quantitative PCR amplification curves of new PCR products obtained for the gDNA of the non-SI sample, an SI sample, and both samples (all as in A ) combined by using specific reverse primers. Forward primer (2015 CDC RT-PCR): 5ʹ-CGGCCACCAACTTGGGATTGAC-3ʹ. Probe: 5ʹ-FAM-GCTGCAAGGAGAAGTCCTGCTGC-TAMRA-3ʹ. Reverse primer (2015 CDC real-time PCR): 5ʹ-CGTACACCGAACCTTTGTCTT-3ʹ. Reverse primer (modified PCR-2): 5ʹ-GTGAGCCCGGCGTT-3ʹ. Abbreviations: ∆Rn, normalized reporter signal; CDC, Centers for Disease Control and Prevention; DDW, double distilled water; RT-PCR, real-time polymerase chain reaction; SI, Solomon Islands.
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    Experimental verification of a 16-compartment digital assay with comparison to the performance of qPCR assays. ( A ) The graph shows the results for the same amount of reference <t>DNA</t> suspended in different elution buffers and quantified with conventional qPCR and with the synergistic PCR algorithm. Tests performed on Applied Biosystems 7500 Fast RT System on the IVD Cytomegalovirus PCR kit (GeneProof) according to the prescription. Elution buffers: (1) water, (2) AE elution buffer QIAamp DNA Mini Kit (Quiagen), (3) MBL5 NucleoMag Blood 200 uL (MACHEREY-NAGEL), (4) <t>MagJET</t> Whole Blood Genomic DNA Kit (Thermo Scientific). The gray line shows the expected distribution of results for Real-Time assay. ( B ) The graph shows the result of 24 runs of the synergistic assay, each on 16 partitions of the amplification mix. We conducted two series of 12 assays on the two elution buffers (1 and 4) that provided the largest difference in the result of the conventional qPCR analysis. The gray line shows the expected distribution of results from the synergistic assay used in the experiment, which should provide 60% precision of assessment. This distribution was also verified using 10,000 Monte-Carlo simulations.
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    Experimental verification of a 16-compartment digital assay with comparison to the performance of qPCR assays. ( A ) The graph shows the results for the same amount of reference <t>DNA</t> suspended in different elution buffers and quantified with conventional qPCR and with the synergistic PCR algorithm. Tests performed on Applied Biosystems 7500 Fast RT System on the IVD Cytomegalovirus PCR kit (GeneProof) according to the prescription. Elution buffers: (1) water, (2) AE elution buffer QIAamp DNA Mini Kit (Quiagen), (3) MBL5 NucleoMag Blood 200 uL (MACHEREY-NAGEL), (4) <t>MagJET</t> Whole Blood Genomic DNA Kit (Thermo Scientific). The gray line shows the expected distribution of results for Real-Time assay. ( B ) The graph shows the result of 24 runs of the synergistic assay, each on 16 partitions of the amplification mix. We conducted two series of 12 assays on the two elution buffers (1 and 4) that provided the largest difference in the result of the conventional qPCR analysis. The gray line shows the expected distribution of results from the synergistic assay used in the experiment, which should provide 60% precision of assessment. This distribution was also verified using 10,000 Monte-Carlo simulations.
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    Experimental verification of a 16-compartment digital assay with comparison to the performance of qPCR assays. ( A ) The graph shows the results for the same amount of reference <t>DNA</t> suspended in different elution buffers and quantified with conventional qPCR and with the synergistic PCR algorithm. Tests performed on Applied Biosystems 7500 Fast RT System on the IVD Cytomegalovirus PCR kit (GeneProof) according to the prescription. Elution buffers: (1) water, (2) AE elution buffer QIAamp DNA Mini Kit (Quiagen), (3) MBL5 NucleoMag Blood 200 uL (MACHEREY-NAGEL), (4) <t>MagJET</t> Whole Blood Genomic DNA Kit (Thermo Scientific). The gray line shows the expected distribution of results for Real-Time assay. ( B ) The graph shows the result of 24 runs of the synergistic assay, each on 16 partitions of the amplification mix. We conducted two series of 12 assays on the two elution buffers (1 and 4) that provided the largest difference in the result of the conventional qPCR analysis. The gray line shows the expected distribution of results from the synergistic assay used in the experiment, which should provide 60% precision of assessment. This distribution was also verified using 10,000 Monte-Carlo simulations.
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    Experimental verification of a 16-compartment digital assay with comparison to the performance of qPCR assays. ( A ) The graph shows the results for the same amount of reference <t>DNA</t> suspended in different elution buffers and quantified with conventional qPCR and with the synergistic PCR algorithm. Tests performed on Applied Biosystems 7500 Fast RT System on the IVD Cytomegalovirus PCR kit (GeneProof) according to the prescription. Elution buffers: (1) water, (2) AE elution buffer QIAamp DNA Mini Kit (Quiagen), (3) MBL5 NucleoMag Blood 200 uL (MACHEREY-NAGEL), (4) <t>MagJET</t> Whole Blood Genomic DNA Kit (Thermo Scientific). The gray line shows the expected distribution of results for Real-Time assay. ( B ) The graph shows the result of 24 runs of the synergistic assay, each on 16 partitions of the amplification mix. We conducted two series of 12 assays on the two elution buffers (1 and 4) that provided the largest difference in the result of the conventional qPCR analysis. The gray line shows the expected distribution of results from the synergistic assay used in the experiment, which should provide 60% precision of assessment. This distribution was also verified using 10,000 Monte-Carlo simulations.
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    Experimental verification of a 16-compartment digital assay with comparison to the performance of qPCR assays. ( A ) The graph shows the results for the same amount of reference <t>DNA</t> suspended in different elution buffers and quantified with conventional qPCR and with the synergistic PCR algorithm. Tests performed on Applied Biosystems 7500 Fast RT System on the IVD Cytomegalovirus PCR kit (GeneProof) according to the prescription. Elution buffers: (1) water, (2) AE elution buffer QIAamp DNA Mini Kit (Quiagen), (3) MBL5 NucleoMag Blood 200 uL (MACHEREY-NAGEL), (4) <t>MagJET</t> Whole Blood Genomic DNA Kit (Thermo Scientific). The gray line shows the expected distribution of results for Real-Time assay. ( B ) The graph shows the result of 24 runs of the synergistic assay, each on 16 partitions of the amplification mix. We conducted two series of 12 assays on the two elution buffers (1 and 4) that provided the largest difference in the result of the conventional qPCR analysis. The gray line shows the expected distribution of results from the synergistic assay used in the experiment, which should provide 60% precision of assessment. This distribution was also verified using 10,000 Monte-Carlo simulations.
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    Experimental verification of a 16-compartment digital assay with comparison to the performance of qPCR assays. ( A ) The graph shows the results for the same amount of reference <t>DNA</t> suspended in different elution buffers and quantified with conventional qPCR and with the synergistic PCR algorithm. Tests performed on Applied Biosystems 7500 Fast RT System on the IVD Cytomegalovirus PCR kit (GeneProof) according to the prescription. Elution buffers: (1) water, (2) AE elution buffer QIAamp DNA Mini Kit (Quiagen), (3) MBL5 NucleoMag Blood 200 uL (MACHEREY-NAGEL), (4) <t>MagJET</t> Whole Blood Genomic DNA Kit (Thermo Scientific). The gray line shows the expected distribution of results for Real-Time assay. ( B ) The graph shows the result of 24 runs of the synergistic assay, each on 16 partitions of the amplification mix. We conducted two series of 12 assays on the two elution buffers (1 and 4) that provided the largest difference in the result of the conventional qPCR analysis. The gray line shows the expected distribution of results from the synergistic assay used in the experiment, which should provide 60% precision of assessment. This distribution was also verified using 10,000 Monte-Carlo simulations.
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    Experimental verification of a 16-compartment digital assay with comparison to the performance of qPCR assays. ( A ) The graph shows the results for the same amount of reference <t>DNA</t> suspended in different elution buffers and quantified with conventional qPCR and with the synergistic PCR algorithm. Tests performed on Applied Biosystems 7500 Fast RT System on the IVD Cytomegalovirus PCR kit (GeneProof) according to the prescription. Elution buffers: (1) water, (2) AE elution buffer QIAamp DNA Mini Kit (Quiagen), (3) MBL5 NucleoMag Blood 200 uL (MACHEREY-NAGEL), (4) <t>MagJET</t> Whole Blood Genomic DNA Kit (Thermo Scientific). The gray line shows the expected distribution of results for Real-Time assay. ( B ) The graph shows the result of 24 runs of the synergistic assay, each on 16 partitions of the amplification mix. We conducted two series of 12 assays on the two elution buffers (1 and 4) that provided the largest difference in the result of the conventional qPCR analysis. The gray line shows the expected distribution of results from the synergistic assay used in the experiment, which should provide 60% precision of assessment. This distribution was also verified using 10,000 Monte-Carlo simulations.
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    Image Search Results


    Gel and quantitative polymerase chain reaction (PCR) assays utilizing both existing and new PCR primers. A , Gel picture showing PCR products for the genomic DNA (gDNA) of a Solomon Islands (SI) sample WP0022.7liq, and a non-SI sample, Treponema pallidum subsp pertenue Gauthier, with the use of the generic forward and reverse primers as indicated. The reverse primer of the Centers for Disease Control and Prevention (CDC) 2015 real-time (RT) PCR assay is labeled reverse primer in Figure 2 . Forward primer (2015 CDC RT-PCR): 5ʹ-CGGCCACCAACTTGGGATTGAC-3ʹ. Reverse primer (2015 CDC RT-PCR): 5ʹ-CGTACACCGAACCTTTGTCTT-3ʹ. Reverse primer (modified PCR-1): 5ʹ- GTGCGGTGAGCCCGGCGTT-3ʹ. Reverse primer (modified PCR-2): 5ʹ-GTGAGCCCGGCGTT-3ʹ. B , Quantitative PCR amplification curves of new PCR products obtained for the gDNA of the non-SI sample, an SI sample, and both samples (all as in A ) combined by using specific reverse primers. Forward primer (2015 CDC RT-PCR): 5ʹ-CGGCCACCAACTTGGGATTGAC-3ʹ. Probe: 5ʹ-FAM-GCTGCAAGGAGAAGTCCTGCTGC-TAMRA-3ʹ. Reverse primer (2015 CDC real-time PCR): 5ʹ-CGTACACCGAACCTTTGTCTT-3ʹ. Reverse primer (modified PCR-2): 5ʹ-GTGAGCCCGGCGTT-3ʹ. Abbreviations: ∆Rn, normalized reporter signal; CDC, Centers for Disease Control and Prevention; DDW, double distilled water; RT-PCR, real-time polymerase chain reaction; SI, Solomon Islands.

    Journal: Clinical Infectious Diseases: An Official Publication of the Infectious Diseases Society of America

    Article Title: Diagnostics for Yaws Eradication: Insights From Direct Next-Generation Sequencing of Cutaneous Strains of Treponema pallidum

    doi: 10.1093/cid/cix892

    Figure Lengend Snippet: Gel and quantitative polymerase chain reaction (PCR) assays utilizing both existing and new PCR primers. A , Gel picture showing PCR products for the genomic DNA (gDNA) of a Solomon Islands (SI) sample WP0022.7liq, and a non-SI sample, Treponema pallidum subsp pertenue Gauthier, with the use of the generic forward and reverse primers as indicated. The reverse primer of the Centers for Disease Control and Prevention (CDC) 2015 real-time (RT) PCR assay is labeled reverse primer in Figure 2 . Forward primer (2015 CDC RT-PCR): 5ʹ-CGGCCACCAACTTGGGATTGAC-3ʹ. Reverse primer (2015 CDC RT-PCR): 5ʹ-CGTACACCGAACCTTTGTCTT-3ʹ. Reverse primer (modified PCR-1): 5ʹ- GTGCGGTGAGCCCGGCGTT-3ʹ. Reverse primer (modified PCR-2): 5ʹ-GTGAGCCCGGCGTT-3ʹ. B , Quantitative PCR amplification curves of new PCR products obtained for the gDNA of the non-SI sample, an SI sample, and both samples (all as in A ) combined by using specific reverse primers. Forward primer (2015 CDC RT-PCR): 5ʹ-CGGCCACCAACTTGGGATTGAC-3ʹ. Probe: 5ʹ-FAM-GCTGCAAGGAGAAGTCCTGCTGC-TAMRA-3ʹ. Reverse primer (2015 CDC real-time PCR): 5ʹ-CGTACACCGAACCTTTGTCTT-3ʹ. Reverse primer (modified PCR-2): 5ʹ-GTGAGCCCGGCGTT-3ʹ. Abbreviations: ∆Rn, normalized reporter signal; CDC, Centers for Disease Control and Prevention; DDW, double distilled water; RT-PCR, real-time polymerase chain reaction; SI, Solomon Islands.

    Article Snippet: Whole-Genome Sequencing and Analysis Treponema pallidum genomic DNA (gDNA) was quantified, per sample, and supplemented with human gDNA (Invitrogen) to make a final total DNA concentration of 500 ng.

    Techniques: Real-time Polymerase Chain Reaction, Polymerase Chain Reaction, Quantitative RT-PCR, Labeling, Reverse Transcription Polymerase Chain Reaction, Modification, Amplification

    Experimental verification of a 16-compartment digital assay with comparison to the performance of qPCR assays. ( A ) The graph shows the results for the same amount of reference DNA suspended in different elution buffers and quantified with conventional qPCR and with the synergistic PCR algorithm. Tests performed on Applied Biosystems 7500 Fast RT System on the IVD Cytomegalovirus PCR kit (GeneProof) according to the prescription. Elution buffers: (1) water, (2) AE elution buffer QIAamp DNA Mini Kit (Quiagen), (3) MBL5 NucleoMag Blood 200 uL (MACHEREY-NAGEL), (4) MagJET Whole Blood Genomic DNA Kit (Thermo Scientific). The gray line shows the expected distribution of results for Real-Time assay. ( B ) The graph shows the result of 24 runs of the synergistic assay, each on 16 partitions of the amplification mix. We conducted two series of 12 assays on the two elution buffers (1 and 4) that provided the largest difference in the result of the conventional qPCR analysis. The gray line shows the expected distribution of results from the synergistic assay used in the experiment, which should provide 60% precision of assessment. This distribution was also verified using 10,000 Monte-Carlo simulations.

    Journal: Scientific Reports

    Article Title: Calibration-free assays on standard real-time PCR devices

    doi: 10.1038/srep44854

    Figure Lengend Snippet: Experimental verification of a 16-compartment digital assay with comparison to the performance of qPCR assays. ( A ) The graph shows the results for the same amount of reference DNA suspended in different elution buffers and quantified with conventional qPCR and with the synergistic PCR algorithm. Tests performed on Applied Biosystems 7500 Fast RT System on the IVD Cytomegalovirus PCR kit (GeneProof) according to the prescription. Elution buffers: (1) water, (2) AE elution buffer QIAamp DNA Mini Kit (Quiagen), (3) MBL5 NucleoMag Blood 200 uL (MACHEREY-NAGEL), (4) MagJET Whole Blood Genomic DNA Kit (Thermo Scientific). The gray line shows the expected distribution of results for Real-Time assay. ( B ) The graph shows the result of 24 runs of the synergistic assay, each on 16 partitions of the amplification mix. We conducted two series of 12 assays on the two elution buffers (1 and 4) that provided the largest difference in the result of the conventional qPCR analysis. The gray line shows the expected distribution of results from the synergistic assay used in the experiment, which should provide 60% precision of assessment. This distribution was also verified using 10,000 Monte-Carlo simulations.

    Article Snippet: Positive control DNA from this kit were diluted in three elution buffers from DNA isolation kits (AE elution buffer QIAamp DNA Mini Kit (Qiagen), MBL5 NucleoMag Blood (MACHEREY-NAGEL) and MagJET Whole Blood Genomic DNA Kit (Thermo Scientific) and water to obtain model samples with 25 000 copies of the target DNA per mL.

    Techniques: Real-time Polymerase Chain Reaction, Polymerase Chain Reaction, Amplification