genomic dna Roche Search Results


  • Logo
  • About
  • News
  • Press Release
  • Team
  • Advisors
  • Partners
  • Contact
  • Bioz Stars
  • Bioz vStars
  • 82
    Roche human genomic dna gdna
    Agarose gel of PCR product and bis-treated PCR product following (A) 15 minutes and (B) 40 minutes of electrophoresis. Size of each PCR product is as indicated. <t>gDNA–untreated</t> genomic <t>DNA;</t> bis gDNA–bis-treated genomic DNA.
    Human Genomic Dna Gdna, supplied by Roche, used in various techniques. Bioz Stars score: 82/100, based on 2 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/human genomic dna gdna/product/Roche
    Average 82 stars, based on 2 article reviews
    Price from $9.99 to $1999.99
    human genomic dna gdna - by Bioz Stars, 2020-02
    82/100 stars
      Buy from Supplier

    gdna  (Roche)
    99
    Roche gdna
    GRN played a pivotal role in the <t>ALD-DNA-induced</t> M2b polarization in vitro . (A–B) Primary macrophages were stimulated with increasing amounts of ALD-DNA for 24 h. mRNA levels of GRN in macrophages were analyzed by real time <t>PCR</t> analysis (A), and protein levels of GRN in the supernatants of macrophages were analyzed by western blot (B). Above, quantitative results of western blots, the band intensity was measured by Image J; Below, representative western blots. Similar results were obtained in three independent experiments. Data are representative of results obtained in three independent experiments. Primary peritoneal macrophages were stimulated by ALD-DNA (50 µg/mL) with GRN (5 µg/mL) for 24 h (C and F), or were pretreated with elastase inhibitor (100 µM) or DMSO (0.1%) for 12 h (D and G), and then were exposed to ALD-DNA, UnALD-DNA, or PBS for another 24 h. Macrophages were transfected with control siRNA (200 nM) or GRN siRNA (siGRN, 200 nM). 36 h posttransfection, macrophages were stimulated with PBS, UnALD-DNA or ALD-DNA (50 µg/mL) (E and H). (C–E) ELISA assay was used to analyze the levels of TNF-α, IL-1β, IL-6, IL-10, IL-12, and MCP-1 in the culture supernatants of macrophages. Data are means ± SD of three independent experiments. (F–H) Western blot analysis was used to analyze the protein levels of iNOS in macrophages. Data are representative of three separate experiments. Similar results were obtained in three independent experiments. Band intensity was measured by Image J and the ratios of iNOS to β-actin were calculated. *, P
    Gdna, supplied by Roche, used in various techniques. Bioz Stars score: 99/100, based on 1094 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/gdna/product/Roche
    Average 99 stars, based on 1094 article reviews
    Price from $9.99 to $1999.99
    gdna - by Bioz Stars, 2020-02
    99/100 stars
      Buy from Supplier

    78
    Roche blood gdna
    GRN played a pivotal role in the <t>ALD-DNA-induced</t> M2b polarization in vitro . (A–B) Primary macrophages were stimulated with increasing amounts of ALD-DNA for 24 h. mRNA levels of GRN in macrophages were analyzed by real time <t>PCR</t> analysis (A), and protein levels of GRN in the supernatants of macrophages were analyzed by western blot (B). Above, quantitative results of western blots, the band intensity was measured by Image J; Below, representative western blots. Similar results were obtained in three independent experiments. Data are representative of results obtained in three independent experiments. Primary peritoneal macrophages were stimulated by ALD-DNA (50 µg/mL) with GRN (5 µg/mL) for 24 h (C and F), or were pretreated with elastase inhibitor (100 µM) or DMSO (0.1%) for 12 h (D and G), and then were exposed to ALD-DNA, UnALD-DNA, or PBS for another 24 h. Macrophages were transfected with control siRNA (200 nM) or GRN siRNA (siGRN, 200 nM). 36 h posttransfection, macrophages were stimulated with PBS, UnALD-DNA or ALD-DNA (50 µg/mL) (E and H). (C–E) ELISA assay was used to analyze the levels of TNF-α, IL-1β, IL-6, IL-10, IL-12, and MCP-1 in the culture supernatants of macrophages. Data are means ± SD of three independent experiments. (F–H) Western blot analysis was used to analyze the protein levels of iNOS in macrophages. Data are representative of three separate experiments. Similar results were obtained in three independent experiments. Band intensity was measured by Image J and the ratios of iNOS to β-actin were calculated. *, P
    Blood Gdna, supplied by Roche, used in various techniques. Bioz Stars score: 78/100, based on 2 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/blood gdna/product/Roche
    Average 78 stars, based on 2 article reviews
    Price from $9.99 to $1999.99
    blood gdna - by Bioz Stars, 2020-02
    78/100 stars
      Buy from Supplier

    90
    Roche sequencing genomic dna gdna
    GRN played a pivotal role in the <t>ALD-DNA-induced</t> M2b polarization in vitro . (A–B) Primary macrophages were stimulated with increasing amounts of ALD-DNA for 24 h. mRNA levels of GRN in macrophages were analyzed by real time <t>PCR</t> analysis (A), and protein levels of GRN in the supernatants of macrophages were analyzed by western blot (B). Above, quantitative results of western blots, the band intensity was measured by Image J; Below, representative western blots. Similar results were obtained in three independent experiments. Data are representative of results obtained in three independent experiments. Primary peritoneal macrophages were stimulated by ALD-DNA (50 µg/mL) with GRN (5 µg/mL) for 24 h (C and F), or were pretreated with elastase inhibitor (100 µM) or DMSO (0.1%) for 12 h (D and G), and then were exposed to ALD-DNA, UnALD-DNA, or PBS for another 24 h. Macrophages were transfected with control siRNA (200 nM) or GRN siRNA (siGRN, 200 nM). 36 h posttransfection, macrophages were stimulated with PBS, UnALD-DNA or ALD-DNA (50 µg/mL) (E and H). (C–E) ELISA assay was used to analyze the levels of TNF-α, IL-1β, IL-6, IL-10, IL-12, and MCP-1 in the culture supernatants of macrophages. Data are means ± SD of three independent experiments. (F–H) Western blot analysis was used to analyze the protein levels of iNOS in macrophages. Data are representative of three separate experiments. Similar results were obtained in three independent experiments. Band intensity was measured by Image J and the ratios of iNOS to β-actin were calculated. *, P
    Sequencing Genomic Dna Gdna, supplied by Roche, used in various techniques. Bioz Stars score: 90/100, based on 7 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/sequencing genomic dna gdna/product/Roche
    Average 90 stars, based on 7 article reviews
    Price from $9.99 to $1999.99
    sequencing genomic dna gdna - by Bioz Stars, 2020-02
    90/100 stars
      Buy from Supplier

    80
    Roche dna extraction genomic dna gdna
    GRN played a pivotal role in the <t>ALD-DNA-induced</t> M2b polarization in vitro . (A–B) Primary macrophages were stimulated with increasing amounts of ALD-DNA for 24 h. mRNA levels of GRN in macrophages were analyzed by real time <t>PCR</t> analysis (A), and protein levels of GRN in the supernatants of macrophages were analyzed by western blot (B). Above, quantitative results of western blots, the band intensity was measured by Image J; Below, representative western blots. Similar results were obtained in three independent experiments. Data are representative of results obtained in three independent experiments. Primary peritoneal macrophages were stimulated by ALD-DNA (50 µg/mL) with GRN (5 µg/mL) for 24 h (C and F), or were pretreated with elastase inhibitor (100 µM) or DMSO (0.1%) for 12 h (D and G), and then were exposed to ALD-DNA, UnALD-DNA, or PBS for another 24 h. Macrophages were transfected with control siRNA (200 nM) or GRN siRNA (siGRN, 200 nM). 36 h posttransfection, macrophages were stimulated with PBS, UnALD-DNA or ALD-DNA (50 µg/mL) (E and H). (C–E) ELISA assay was used to analyze the levels of TNF-α, IL-1β, IL-6, IL-10, IL-12, and MCP-1 in the culture supernatants of macrophages. Data are means ± SD of three independent experiments. (F–H) Western blot analysis was used to analyze the protein levels of iNOS in macrophages. Data are representative of three separate experiments. Similar results were obtained in three independent experiments. Band intensity was measured by Image J and the ratios of iNOS to β-actin were calculated. *, P
    Dna Extraction Genomic Dna Gdna, supplied by Roche, used in various techniques. Bioz Stars score: 80/100, based on 7 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/dna extraction genomic dna gdna/product/Roche
    Average 80 stars, based on 7 article reviews
    Price from $9.99 to $1999.99
    dna extraction genomic dna gdna - by Bioz Stars, 2020-02
    80/100 stars
      Buy from Supplier

    77
    Roche human gdna standard
    GRN played a pivotal role in the <t>ALD-DNA-induced</t> M2b polarization in vitro . (A–B) Primary macrophages were stimulated with increasing amounts of ALD-DNA for 24 h. mRNA levels of GRN in macrophages were analyzed by real time <t>PCR</t> analysis (A), and protein levels of GRN in the supernatants of macrophages were analyzed by western blot (B). Above, quantitative results of western blots, the band intensity was measured by Image J; Below, representative western blots. Similar results were obtained in three independent experiments. Data are representative of results obtained in three independent experiments. Primary peritoneal macrophages were stimulated by ALD-DNA (50 µg/mL) with GRN (5 µg/mL) for 24 h (C and F), or were pretreated with elastase inhibitor (100 µM) or DMSO (0.1%) for 12 h (D and G), and then were exposed to ALD-DNA, UnALD-DNA, or PBS for another 24 h. Macrophages were transfected with control siRNA (200 nM) or GRN siRNA (siGRN, 200 nM). 36 h posttransfection, macrophages were stimulated with PBS, UnALD-DNA or ALD-DNA (50 µg/mL) (E and H). (C–E) ELISA assay was used to analyze the levels of TNF-α, IL-1β, IL-6, IL-10, IL-12, and MCP-1 in the culture supernatants of macrophages. Data are means ± SD of three independent experiments. (F–H) Western blot analysis was used to analyze the protein levels of iNOS in macrophages. Data are representative of three separate experiments. Similar results were obtained in three independent experiments. Band intensity was measured by Image J and the ratios of iNOS to β-actin were calculated. *, P
    Human Gdna Standard, supplied by Roche, used in various techniques. Bioz Stars score: 77/100, based on 3 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/human gdna standard/product/Roche
    Average 77 stars, based on 3 article reviews
    Price from $9.99 to $1999.99
    human gdna standard - by Bioz Stars, 2020-02
    77/100 stars
      Buy from Supplier

    82
    Roche human gdna
    GRN played a pivotal role in the <t>ALD-DNA-induced</t> M2b polarization in vitro . (A–B) Primary macrophages were stimulated with increasing amounts of ALD-DNA for 24 h. mRNA levels of GRN in macrophages were analyzed by real time <t>PCR</t> analysis (A), and protein levels of GRN in the supernatants of macrophages were analyzed by western blot (B). Above, quantitative results of western blots, the band intensity was measured by Image J; Below, representative western blots. Similar results were obtained in three independent experiments. Data are representative of results obtained in three independent experiments. Primary peritoneal macrophages were stimulated by ALD-DNA (50 µg/mL) with GRN (5 µg/mL) for 24 h (C and F), or were pretreated with elastase inhibitor (100 µM) or DMSO (0.1%) for 12 h (D and G), and then were exposed to ALD-DNA, UnALD-DNA, or PBS for another 24 h. Macrophages were transfected with control siRNA (200 nM) or GRN siRNA (siGRN, 200 nM). 36 h posttransfection, macrophages were stimulated with PBS, UnALD-DNA or ALD-DNA (50 µg/mL) (E and H). (C–E) ELISA assay was used to analyze the levels of TNF-α, IL-1β, IL-6, IL-10, IL-12, and MCP-1 in the culture supernatants of macrophages. Data are means ± SD of three independent experiments. (F–H) Western blot analysis was used to analyze the protein levels of iNOS in macrophages. Data are representative of three separate experiments. Similar results were obtained in three independent experiments. Band intensity was measured by Image J and the ratios of iNOS to β-actin were calculated. *, P
    Human Gdna, supplied by Roche, used in various techniques. Bioz Stars score: 82/100, based on 8 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/human gdna/product/Roche
    Average 82 stars, based on 8 article reviews
    Price from $9.99 to $1999.99
    human gdna - by Bioz Stars, 2020-02
    82/100 stars
      Buy from Supplier

    79
    Roche high molecular weight gdna
    GRN played a pivotal role in the <t>ALD-DNA-induced</t> M2b polarization in vitro . (A–B) Primary macrophages were stimulated with increasing amounts of ALD-DNA for 24 h. mRNA levels of GRN in macrophages were analyzed by real time <t>PCR</t> analysis (A), and protein levels of GRN in the supernatants of macrophages were analyzed by western blot (B). Above, quantitative results of western blots, the band intensity was measured by Image J; Below, representative western blots. Similar results were obtained in three independent experiments. Data are representative of results obtained in three independent experiments. Primary peritoneal macrophages were stimulated by ALD-DNA (50 µg/mL) with GRN (5 µg/mL) for 24 h (C and F), or were pretreated with elastase inhibitor (100 µM) or DMSO (0.1%) for 12 h (D and G), and then were exposed to ALD-DNA, UnALD-DNA, or PBS for another 24 h. Macrophages were transfected with control siRNA (200 nM) or GRN siRNA (siGRN, 200 nM). 36 h posttransfection, macrophages were stimulated with PBS, UnALD-DNA or ALD-DNA (50 µg/mL) (E and H). (C–E) ELISA assay was used to analyze the levels of TNF-α, IL-1β, IL-6, IL-10, IL-12, and MCP-1 in the culture supernatants of macrophages. Data are means ± SD of three independent experiments. (F–H) Western blot analysis was used to analyze the protein levels of iNOS in macrophages. Data are representative of three separate experiments. Similar results were obtained in three independent experiments. Band intensity was measured by Image J and the ratios of iNOS to β-actin were calculated. *, P
    High Molecular Weight Gdna, supplied by Roche, used in various techniques. Bioz Stars score: 79/100, based on 11 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/high molecular weight gdna/product/Roche
    Average 79 stars, based on 11 article reviews
    Price from $9.99 to $1999.99
    high molecular weight gdna - by Bioz Stars, 2020-02
    79/100 stars
      Buy from Supplier

    79
    Roche human buffy coat gdna
    GRN played a pivotal role in the <t>ALD-DNA-induced</t> M2b polarization in vitro . (A–B) Primary macrophages were stimulated with increasing amounts of ALD-DNA for 24 h. mRNA levels of GRN in macrophages were analyzed by real time <t>PCR</t> analysis (A), and protein levels of GRN in the supernatants of macrophages were analyzed by western blot (B). Above, quantitative results of western blots, the band intensity was measured by Image J; Below, representative western blots. Similar results were obtained in three independent experiments. Data are representative of results obtained in three independent experiments. Primary peritoneal macrophages were stimulated by ALD-DNA (50 µg/mL) with GRN (5 µg/mL) for 24 h (C and F), or were pretreated with elastase inhibitor (100 µM) or DMSO (0.1%) for 12 h (D and G), and then were exposed to ALD-DNA, UnALD-DNA, or PBS for another 24 h. Macrophages were transfected with control siRNA (200 nM) or GRN siRNA (siGRN, 200 nM). 36 h posttransfection, macrophages were stimulated with PBS, UnALD-DNA or ALD-DNA (50 µg/mL) (E and H). (C–E) ELISA assay was used to analyze the levels of TNF-α, IL-1β, IL-6, IL-10, IL-12, and MCP-1 in the culture supernatants of macrophages. Data are means ± SD of three independent experiments. (F–H) Western blot analysis was used to analyze the protein levels of iNOS in macrophages. Data are representative of three separate experiments. Similar results were obtained in three independent experiments. Band intensity was measured by Image J and the ratios of iNOS to β-actin were calculated. *, P
    Human Buffy Coat Gdna, supplied by Roche, used in various techniques. Bioz Stars score: 79/100, based on 4 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/human buffy coat gdna/product/Roche
    Average 79 stars, based on 4 article reviews
    Price from $9.99 to $1999.99
    human buffy coat gdna - by Bioz Stars, 2020-02
    79/100 stars
      Buy from Supplier

    77
    Roche unmethylated genomic dna
    (A) Methylation‐specific melting curve analysis for the IMiD‐sensitive and IMiD‐resistant cell lines, showing the absence of promoter <t>DNA</t> methylation of CRBN. The blue curves are the fully unmethylated and fully methylated controls (left and right curve, respectively), and the green lines represent the cell samples. (B, C) ChIP for H3K4me3, H3K27Ac, and H3K27me3 around the transcription site of CRBN for OPM2 and NCI‐H929, respectively, revealed that IMiD‐resistant cell lines retained their activating histone marks (H3K4me3, H3K27Ac) without any significant increase in the repressive mark H3K27me3. (D) Chromatin accessibility values for the probes mapping to CRBN, assessed with Acce <t>SssI</t> ble. The difference in the accessibility of the resistant cell lines compared with their parental cell line (ΔAcc) was calculated by subtracting the accessibility value of the paternal cell line from the accessibility value of the resistant cell line for each individual probe. None of the probes covering CRBN showed significant accessibility changes in any of the IMiD‐resistant cell lines. (E, F) Kernel density plot of the combined DNA methylation changes and chromatin accessibility changes observed in the IMiD‐resistant cell lines. The OPM2 IMiD‐resistant cells exhibit primarily a decrease in the global chromatin accessibility when compared with the parental OPM2 (E), while H929 IMiD‐resistant cell lines exhibit a pattern of combined loss of chromatin accessibility with increased DNA methylation (F).
    Unmethylated Genomic Dna, supplied by Roche, used in various techniques. Bioz Stars score: 77/100, based on 3 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/unmethylated genomic dna/product/Roche
    Average 77 stars, based on 3 article reviews
    Price from $9.99 to $1999.99
    unmethylated genomic dna - by Bioz Stars, 2020-02
    77/100 stars
      Buy from Supplier

    88
    Roche genomic dna kit
    (A) Methylation‐specific melting curve analysis for the IMiD‐sensitive and IMiD‐resistant cell lines, showing the absence of promoter <t>DNA</t> methylation of CRBN. The blue curves are the fully unmethylated and fully methylated controls (left and right curve, respectively), and the green lines represent the cell samples. (B, C) ChIP for H3K4me3, H3K27Ac, and H3K27me3 around the transcription site of CRBN for OPM2 and NCI‐H929, respectively, revealed that IMiD‐resistant cell lines retained their activating histone marks (H3K4me3, H3K27Ac) without any significant increase in the repressive mark H3K27me3. (D) Chromatin accessibility values for the probes mapping to CRBN, assessed with Acce <t>SssI</t> ble. The difference in the accessibility of the resistant cell lines compared with their parental cell line (ΔAcc) was calculated by subtracting the accessibility value of the paternal cell line from the accessibility value of the resistant cell line for each individual probe. None of the probes covering CRBN showed significant accessibility changes in any of the IMiD‐resistant cell lines. (E, F) Kernel density plot of the combined DNA methylation changes and chromatin accessibility changes observed in the IMiD‐resistant cell lines. The OPM2 IMiD‐resistant cells exhibit primarily a decrease in the global chromatin accessibility when compared with the parental OPM2 (E), while H929 IMiD‐resistant cell lines exhibit a pattern of combined loss of chromatin accessibility with increased DNA methylation (F).
    Genomic Dna Kit, supplied by Roche, used in various techniques. Bioz Stars score: 88/100, based on 12 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/genomic dna kit/product/Roche
    Average 88 stars, based on 12 article reviews
    Price from $9.99 to $1999.99
    genomic dna kit - by Bioz Stars, 2020-02
    88/100 stars
      Buy from Supplier

    78
    Roche normal genomic dna
    (A) Methylation‐specific melting curve analysis for the IMiD‐sensitive and IMiD‐resistant cell lines, showing the absence of promoter <t>DNA</t> methylation of CRBN. The blue curves are the fully unmethylated and fully methylated controls (left and right curve, respectively), and the green lines represent the cell samples. (B, C) ChIP for H3K4me3, H3K27Ac, and H3K27me3 around the transcription site of CRBN for OPM2 and NCI‐H929, respectively, revealed that IMiD‐resistant cell lines retained their activating histone marks (H3K4me3, H3K27Ac) without any significant increase in the repressive mark H3K27me3. (D) Chromatin accessibility values for the probes mapping to CRBN, assessed with Acce <t>SssI</t> ble. The difference in the accessibility of the resistant cell lines compared with their parental cell line (ΔAcc) was calculated by subtracting the accessibility value of the paternal cell line from the accessibility value of the resistant cell line for each individual probe. None of the probes covering CRBN showed significant accessibility changes in any of the IMiD‐resistant cell lines. (E, F) Kernel density plot of the combined DNA methylation changes and chromatin accessibility changes observed in the IMiD‐resistant cell lines. The OPM2 IMiD‐resistant cells exhibit primarily a decrease in the global chromatin accessibility when compared with the parental OPM2 (E), while H929 IMiD‐resistant cell lines exhibit a pattern of combined loss of chromatin accessibility with increased DNA methylation (F).
    Normal Genomic Dna, supplied by Roche, used in various techniques. Bioz Stars score: 78/100, based on 2 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/normal genomic dna/product/Roche
    Average 78 stars, based on 2 article reviews
    Price from $9.99 to $1999.99
    normal genomic dna - by Bioz Stars, 2020-02
    78/100 stars
      Buy from Supplier

    80
    Roche dog genomic dna
    (A) Methylation‐specific melting curve analysis for the IMiD‐sensitive and IMiD‐resistant cell lines, showing the absence of promoter <t>DNA</t> methylation of CRBN. The blue curves are the fully unmethylated and fully methylated controls (left and right curve, respectively), and the green lines represent the cell samples. (B, C) ChIP for H3K4me3, H3K27Ac, and H3K27me3 around the transcription site of CRBN for OPM2 and NCI‐H929, respectively, revealed that IMiD‐resistant cell lines retained their activating histone marks (H3K4me3, H3K27Ac) without any significant increase in the repressive mark H3K27me3. (D) Chromatin accessibility values for the probes mapping to CRBN, assessed with Acce <t>SssI</t> ble. The difference in the accessibility of the resistant cell lines compared with their parental cell line (ΔAcc) was calculated by subtracting the accessibility value of the paternal cell line from the accessibility value of the resistant cell line for each individual probe. None of the probes covering CRBN showed significant accessibility changes in any of the IMiD‐resistant cell lines. (E, F) Kernel density plot of the combined DNA methylation changes and chromatin accessibility changes observed in the IMiD‐resistant cell lines. The OPM2 IMiD‐resistant cells exhibit primarily a decrease in the global chromatin accessibility when compared with the parental OPM2 (E), while H929 IMiD‐resistant cell lines exhibit a pattern of combined loss of chromatin accessibility with increased DNA methylation (F).
    Dog Genomic Dna, supplied by Roche, used in various techniques. Bioz Stars score: 80/100, based on 9 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/dog genomic dna/product/Roche
    Average 80 stars, based on 9 article reviews
    Price from $9.99 to $1999.99
    dog genomic dna - by Bioz Stars, 2020-02
    80/100 stars
      Buy from Supplier

    99
    Eurofins genomic dna
    (A) Methylation‐specific melting curve analysis for the IMiD‐sensitive and IMiD‐resistant cell lines, showing the absence of promoter <t>DNA</t> methylation of CRBN. The blue curves are the fully unmethylated and fully methylated controls (left and right curve, respectively), and the green lines represent the cell samples. (B, C) ChIP for H3K4me3, H3K27Ac, and H3K27me3 around the transcription site of CRBN for OPM2 and NCI‐H929, respectively, revealed that IMiD‐resistant cell lines retained their activating histone marks (H3K4me3, H3K27Ac) without any significant increase in the repressive mark H3K27me3. (D) Chromatin accessibility values for the probes mapping to CRBN, assessed with Acce <t>SssI</t> ble. The difference in the accessibility of the resistant cell lines compared with their parental cell line (ΔAcc) was calculated by subtracting the accessibility value of the paternal cell line from the accessibility value of the resistant cell line for each individual probe. None of the probes covering CRBN showed significant accessibility changes in any of the IMiD‐resistant cell lines. (E, F) Kernel density plot of the combined DNA methylation changes and chromatin accessibility changes observed in the IMiD‐resistant cell lines. The OPM2 IMiD‐resistant cells exhibit primarily a decrease in the global chromatin accessibility when compared with the parental OPM2 (E), while H929 IMiD‐resistant cell lines exhibit a pattern of combined loss of chromatin accessibility with increased DNA methylation (F).
    Genomic Dna, supplied by Eurofins, used in various techniques. Bioz Stars score: 99/100, based on 87 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/genomic dna/product/Eurofins
    Average 99 stars, based on 87 article reviews
    Price from $9.99 to $1999.99
    genomic dna - by Bioz Stars, 2020-02
    99/100 stars
      Buy from Supplier

    80
    Roche genomic dna isolation genomic dna
    (A) Methylation‐specific melting curve analysis for the IMiD‐sensitive and IMiD‐resistant cell lines, showing the absence of promoter <t>DNA</t> methylation of CRBN. The blue curves are the fully unmethylated and fully methylated controls (left and right curve, respectively), and the green lines represent the cell samples. (B, C) ChIP for H3K4me3, H3K27Ac, and H3K27me3 around the transcription site of CRBN for OPM2 and NCI‐H929, respectively, revealed that IMiD‐resistant cell lines retained their activating histone marks (H3K4me3, H3K27Ac) without any significant increase in the repressive mark H3K27me3. (D) Chromatin accessibility values for the probes mapping to CRBN, assessed with Acce <t>SssI</t> ble. The difference in the accessibility of the resistant cell lines compared with their parental cell line (ΔAcc) was calculated by subtracting the accessibility value of the paternal cell line from the accessibility value of the resistant cell line for each individual probe. None of the probes covering CRBN showed significant accessibility changes in any of the IMiD‐resistant cell lines. (E, F) Kernel density plot of the combined DNA methylation changes and chromatin accessibility changes observed in the IMiD‐resistant cell lines. The OPM2 IMiD‐resistant cells exhibit primarily a decrease in the global chromatin accessibility when compared with the parental OPM2 (E), while H929 IMiD‐resistant cell lines exhibit a pattern of combined loss of chromatin accessibility with increased DNA methylation (F).
    Genomic Dna Isolation Genomic Dna, supplied by Roche, used in various techniques. Bioz Stars score: 80/100, based on 4 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/genomic dna isolation genomic dna/product/Roche
    Average 80 stars, based on 4 article reviews
    Price from $9.99 to $1999.99
    genomic dna isolation genomic dna - by Bioz Stars, 2020-02
    80/100 stars
      Buy from Supplier

    77
    Roche bacteriophage genomic dna templates
    Real-time PCR detection of the amplification of a 533-bp fragment of the <t>DNA</t> packaging protein of enterobacteria phage λ using TaqMan® probe detection. Reactions were performed in quadruplicate and compared the performance of unmodified and double OXP-modified primers for their ability to detect 10, 50, 100, 500, 1000, 5000 and 10 000 copies of <t>λ</t> genomic DNA. This figure displays the amplification plots for unmodified ( A ) and double OXP-modified ( B ) primers as well as the corresponding standard curve ( C ).
    Bacteriophage Genomic Dna Templates, supplied by Roche, used in various techniques. Bioz Stars score: 77/100, based on 5 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/bacteriophage genomic dna templates/product/Roche
    Average 77 stars, based on 5 article reviews
    Price from $9.99 to $1999.99
    bacteriophage genomic dna templates - by Bioz Stars, 2020-02
    77/100 stars
      Buy from Supplier

    77
    Roche viral genomic dna purification kit
    Real-time PCR detection of the amplification of a 533-bp fragment of the <t>DNA</t> packaging protein of enterobacteria phage λ using TaqMan® probe detection. Reactions were performed in quadruplicate and compared the performance of unmodified and double OXP-modified primers for their ability to detect 10, 50, 100, 500, 1000, 5000 and 10 000 copies of <t>λ</t> genomic DNA. This figure displays the amplification plots for unmodified ( A ) and double OXP-modified ( B ) primers as well as the corresponding standard curve ( C ).
    Viral Genomic Dna Purification Kit, supplied by Roche, used in various techniques. Bioz Stars score: 77/100, based on 11 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/viral genomic dna purification kit/product/Roche
    Average 77 stars, based on 11 article reviews
    Price from $9.99 to $1999.99
    viral genomic dna purification kit - by Bioz Stars, 2020-02
    77/100 stars
      Buy from Supplier

    80
    Roche bacterial genomic dna purification kit
    Real-time PCR detection of the amplification of a 533-bp fragment of the <t>DNA</t> packaging protein of enterobacteria phage λ using TaqMan® probe detection. Reactions were performed in quadruplicate and compared the performance of unmodified and double OXP-modified primers for their ability to detect 10, 50, 100, 500, 1000, 5000 and 10 000 copies of <t>λ</t> genomic DNA. This figure displays the amplification plots for unmodified ( A ) and double OXP-modified ( B ) primers as well as the corresponding standard curve ( C ).
    Bacterial Genomic Dna Purification Kit, supplied by Roche, used in various techniques. Bioz Stars score: 80/100, based on 6 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/bacterial genomic dna purification kit/product/Roche
    Average 80 stars, based on 6 article reviews
    Price from $9.99 to $1999.99
    bacterial genomic dna purification kit - by Bioz Stars, 2020-02
    80/100 stars
      Buy from Supplier

    81
    ATUM biotin labelled genomic dna
    Real-time PCR detection of the amplification of a 533-bp fragment of the <t>DNA</t> packaging protein of enterobacteria phage λ using TaqMan® probe detection. Reactions were performed in quadruplicate and compared the performance of unmodified and double OXP-modified primers for their ability to detect 10, 50, 100, 500, 1000, 5000 and 10 000 copies of <t>λ</t> genomic DNA. This figure displays the amplification plots for unmodified ( A ) and double OXP-modified ( B ) primers as well as the corresponding standard curve ( C ).
    Biotin Labelled Genomic Dna, supplied by ATUM, used in various techniques. Bioz Stars score: 81/100, based on 7 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/biotin labelled genomic dna/product/ATUM
    Average 81 stars, based on 7 article reviews
    Price from $9.99 to $1999.99
    biotin labelled genomic dna - by Bioz Stars, 2020-02
    81/100 stars
      Buy from Supplier

    83
    Roche genomic dna extraction peripheral blood genomic dna
    Real-time PCR detection of the amplification of a 533-bp fragment of the <t>DNA</t> packaging protein of enterobacteria phage λ using TaqMan® probe detection. Reactions were performed in quadruplicate and compared the performance of unmodified and double OXP-modified primers for their ability to detect 10, 50, 100, 500, 1000, 5000 and 10 000 copies of <t>λ</t> genomic DNA. This figure displays the amplification plots for unmodified ( A ) and double OXP-modified ( B ) primers as well as the corresponding standard curve ( C ).
    Genomic Dna Extraction Peripheral Blood Genomic Dna, supplied by Roche, used in various techniques. Bioz Stars score: 83/100, based on 3 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/genomic dna extraction peripheral blood genomic dna/product/Roche
    Average 83 stars, based on 3 article reviews
    Price from $9.99 to $1999.99
    genomic dna extraction peripheral blood genomic dna - by Bioz Stars, 2020-02
    83/100 stars
      Buy from Supplier

    Image Search Results


    Agarose gel of PCR product and bis-treated PCR product following (A) 15 minutes and (B) 40 minutes of electrophoresis. Size of each PCR product is as indicated. gDNA–untreated genomic DNA; bis gDNA–bis-treated genomic DNA.

    Journal: PLoS ONE

    Article Title: Circulating cell-free DNA from plasma undergoes less fragmentation during bisulfite treatment than genomic DNA due to low molecular weight

    doi: 10.1371/journal.pone.0224338

    Figure Lengend Snippet: Agarose gel of PCR product and bis-treated PCR product following (A) 15 minutes and (B) 40 minutes of electrophoresis. Size of each PCR product is as indicated. gDNA–untreated genomic DNA; bis gDNA–bis-treated genomic DNA.

    Article Snippet: Human genomic DNA (gDNA) purchased from Roche (Cat #11691112001) and stored at 4°C as specified by the manufacturer was used as the high molecular weight DNA sample.

    Techniques: Agarose Gel Electrophoresis, Polymerase Chain Reaction, Electrophoresis

    Agarose gel of cirDNA and bis-treated cirDNA following (A) 15 minutes and (B) 40 minutes of electrophoresis. The amount of cirDNA in each lane corresponds to the total cirDNA extracted from the plasma volumes indicated. gDNA–untreated genomic DNA.

    Journal: PLoS ONE

    Article Title: Circulating cell-free DNA from plasma undergoes less fragmentation during bisulfite treatment than genomic DNA due to low molecular weight

    doi: 10.1371/journal.pone.0224338

    Figure Lengend Snippet: Agarose gel of cirDNA and bis-treated cirDNA following (A) 15 minutes and (B) 40 minutes of electrophoresis. The amount of cirDNA in each lane corresponds to the total cirDNA extracted from the plasma volumes indicated. gDNA–untreated genomic DNA.

    Article Snippet: Human genomic DNA (gDNA) purchased from Roche (Cat #11691112001) and stored at 4°C as specified by the manufacturer was used as the high molecular weight DNA sample.

    Techniques: Agarose Gel Electrophoresis, Electrophoresis

    GRN played a pivotal role in the ALD-DNA-induced M2b polarization in vitro . (A–B) Primary macrophages were stimulated with increasing amounts of ALD-DNA for 24 h. mRNA levels of GRN in macrophages were analyzed by real time PCR analysis (A), and protein levels of GRN in the supernatants of macrophages were analyzed by western blot (B). Above, quantitative results of western blots, the band intensity was measured by Image J; Below, representative western blots. Similar results were obtained in three independent experiments. Data are representative of results obtained in three independent experiments. Primary peritoneal macrophages were stimulated by ALD-DNA (50 µg/mL) with GRN (5 µg/mL) for 24 h (C and F), or were pretreated with elastase inhibitor (100 µM) or DMSO (0.1%) for 12 h (D and G), and then were exposed to ALD-DNA, UnALD-DNA, or PBS for another 24 h. Macrophages were transfected with control siRNA (200 nM) or GRN siRNA (siGRN, 200 nM). 36 h posttransfection, macrophages were stimulated with PBS, UnALD-DNA or ALD-DNA (50 µg/mL) (E and H). (C–E) ELISA assay was used to analyze the levels of TNF-α, IL-1β, IL-6, IL-10, IL-12, and MCP-1 in the culture supernatants of macrophages. Data are means ± SD of three independent experiments. (F–H) Western blot analysis was used to analyze the protein levels of iNOS in macrophages. Data are representative of three separate experiments. Similar results were obtained in three independent experiments. Band intensity was measured by Image J and the ratios of iNOS to β-actin were calculated. *, P

    Journal: PLoS ONE

    Article Title: Granulin Exacerbates Lupus Nephritis via Enhancing Macrophage M2b Polarization

    doi: 10.1371/journal.pone.0065542

    Figure Lengend Snippet: GRN played a pivotal role in the ALD-DNA-induced M2b polarization in vitro . (A–B) Primary macrophages were stimulated with increasing amounts of ALD-DNA for 24 h. mRNA levels of GRN in macrophages were analyzed by real time PCR analysis (A), and protein levels of GRN in the supernatants of macrophages were analyzed by western blot (B). Above, quantitative results of western blots, the band intensity was measured by Image J; Below, representative western blots. Similar results were obtained in three independent experiments. Data are representative of results obtained in three independent experiments. Primary peritoneal macrophages were stimulated by ALD-DNA (50 µg/mL) with GRN (5 µg/mL) for 24 h (C and F), or were pretreated with elastase inhibitor (100 µM) or DMSO (0.1%) for 12 h (D and G), and then were exposed to ALD-DNA, UnALD-DNA, or PBS for another 24 h. Macrophages were transfected with control siRNA (200 nM) or GRN siRNA (siGRN, 200 nM). 36 h posttransfection, macrophages were stimulated with PBS, UnALD-DNA or ALD-DNA (50 µg/mL) (E and H). (C–E) ELISA assay was used to analyze the levels of TNF-α, IL-1β, IL-6, IL-10, IL-12, and MCP-1 in the culture supernatants of macrophages. Data are means ± SD of three independent experiments. (F–H) Western blot analysis was used to analyze the protein levels of iNOS in macrophages. Data are representative of three separate experiments. Similar results were obtained in three independent experiments. Band intensity was measured by Image J and the ratios of iNOS to β-actin were calculated. *, P

    Article Snippet: Genomic DNA was subjected to quantitative real-time PCR using a Lightcycler480 and SYBR Green system (Roche Diagnostic Systems) following the manufacturer’s protocol.

    Techniques: In Vitro, Real-time Polymerase Chain Reaction, Western Blot, Transfection, Enzyme-linked Immunosorbent Assay

    GRN aggravated lupus nephritis in lupus model by enhancing ALD-DNA-induced M2b polarization. CD11b + /F4/80 high renal macrophages were sorted from nephritic single-cell suspensions from pGRN-treated, pcDNA3.1-treated, or LV-shGRN-injected, LV-shNC-injected lupus model by flow cytometry. (A) mRNA levels of TNF-α , IL-1β , IL-6 , IL-10 , IL-12 , MCP-1 , Nos2 (iNOS) and Agr1 in the renal macrophages screened from pGRN or pcDNA3.1-treated lupus mice were evaluated by real-time PCR. Data are means ± SD from 8 mice in each group. (B) Real time analysis for the mRNA levels of TNF-α , IL-1β , IL-6 , IL-10 , IL-12 , MCP-1 , Nos2 (iNOS) and Agr1 in renal macrophages from LV-shGRN-injected lupus mice and control mice. Data are means ± SD from 8 mice in each group. *, P

    Journal: PLoS ONE

    Article Title: Granulin Exacerbates Lupus Nephritis via Enhancing Macrophage M2b Polarization

    doi: 10.1371/journal.pone.0065542

    Figure Lengend Snippet: GRN aggravated lupus nephritis in lupus model by enhancing ALD-DNA-induced M2b polarization. CD11b + /F4/80 high renal macrophages were sorted from nephritic single-cell suspensions from pGRN-treated, pcDNA3.1-treated, or LV-shGRN-injected, LV-shNC-injected lupus model by flow cytometry. (A) mRNA levels of TNF-α , IL-1β , IL-6 , IL-10 , IL-12 , MCP-1 , Nos2 (iNOS) and Agr1 in the renal macrophages screened from pGRN or pcDNA3.1-treated lupus mice were evaluated by real-time PCR. Data are means ± SD from 8 mice in each group. (B) Real time analysis for the mRNA levels of TNF-α , IL-1β , IL-6 , IL-10 , IL-12 , MCP-1 , Nos2 (iNOS) and Agr1 in renal macrophages from LV-shGRN-injected lupus mice and control mice. Data are means ± SD from 8 mice in each group. *, P

    Article Snippet: Genomic DNA was subjected to quantitative real-time PCR using a Lightcycler480 and SYBR Green system (Roche Diagnostic Systems) following the manufacturer’s protocol.

    Techniques: Injection, Flow Cytometry, Cytometry, Mouse Assay, Real-time Polymerase Chain Reaction

    PCR amplification of class 1 integron variable regions in ESBL producing Klebsiella pneumoniae isolates (Lanes 1–5 and 7–12). Lane 6, DNA molecular weight marker (100 bp, Fermentas).

    Journal: Brazilian Journal of Microbiology

    Article Title: Integron mediated multidrug resistance in extended spectrum beta-lactamase producing clinical isolates of Klebsiella pneumoniae

    doi:

    Figure Lengend Snippet: PCR amplification of class 1 integron variable regions in ESBL producing Klebsiella pneumoniae isolates (Lanes 1–5 and 7–12). Lane 6, DNA molecular weight marker (100 bp, Fermentas).

    Article Snippet: Detection of class 1, 2 and 3 integrons by PCR Isolates were grown overnight (18 h) in Luria Bertani (LB) broth (Difco, Detroit, MI, USA) at 37 °C and genomic DNA was extracted using High Pure PCR template Prep kit for Genomic DNA extraction (Roche Diagnostics, Mannheim, Germany).

    Techniques: Polymerase Chain Reaction, Amplification, Molecular Weight, Marker

    PCR amplification product of class 1 (A) and class 2 (B) integrase genes in a number of ESBL producing Klebsiella pneumoniae clinical isolates. Ladder, DNA molecular weight marker (100 bp, Fermentas); C+, positive control for class 1 integron; C − , negative control (no DNA template).

    Journal: Brazilian Journal of Microbiology

    Article Title: Integron mediated multidrug resistance in extended spectrum beta-lactamase producing clinical isolates of Klebsiella pneumoniae

    doi:

    Figure Lengend Snippet: PCR amplification product of class 1 (A) and class 2 (B) integrase genes in a number of ESBL producing Klebsiella pneumoniae clinical isolates. Ladder, DNA molecular weight marker (100 bp, Fermentas); C+, positive control for class 1 integron; C − , negative control (no DNA template).

    Article Snippet: Detection of class 1, 2 and 3 integrons by PCR Isolates were grown overnight (18 h) in Luria Bertani (LB) broth (Difco, Detroit, MI, USA) at 37 °C and genomic DNA was extracted using High Pure PCR template Prep kit for Genomic DNA extraction (Roche Diagnostics, Mannheim, Germany).

    Techniques: Polymerase Chain Reaction, Amplification, Molecular Weight, Marker, Positive Control, Negative Control

    Distribution of cytokeratine (CK; (a)–(c)) and DNA methyltransferase 3A (DNMT3A; (d)–(f)) immunoreactive HT29 cells. (a) and (d) x -axes represent the intensities of immunoreactions from negative (−) to strong (+++). y -axes represent the distribution (percentage) of cells showing different immunoreaction intensities (blue columns: control cells; red columns: type 3. DNA-treated cells). (b) and (c) CK expression before (b) and after (c) type 3. DNA incubation (immunoreactive cells/arrows/display brownish cytoplasmic reaction, 200x magnification, hematoxylin costaining). (e) and (f) DNMT3A expression before (e) and after (f) type 3. DNA incubation (immunoreactive cells/arrows/displayed brownish cytoplasmic reaction, 200x magnification, hematoxylin costaining).

    Journal: The Scientific World Journal

    Article Title: Association of Self-DNA Mediated TLR9-Related Gene, DNA Methyltransferase, and Cytokeratin Protein Expression Alterations in HT29-Cells to DNA Fragment Length and Methylation Status

    doi: 10.1155/2013/293296

    Figure Lengend Snippet: Distribution of cytokeratine (CK; (a)–(c)) and DNA methyltransferase 3A (DNMT3A; (d)–(f)) immunoreactive HT29 cells. (a) and (d) x -axes represent the intensities of immunoreactions from negative (−) to strong (+++). y -axes represent the distribution (percentage) of cells showing different immunoreaction intensities (blue columns: control cells; red columns: type 3. DNA-treated cells). (b) and (c) CK expression before (b) and after (c) type 3. DNA incubation (immunoreactive cells/arrows/display brownish cytoplasmic reaction, 200x magnification, hematoxylin costaining). (e) and (f) DNMT3A expression before (e) and after (f) type 3. DNA incubation (immunoreactive cells/arrows/displayed brownish cytoplasmic reaction, 200x magnification, hematoxylin costaining).

    Article Snippet: Isolation, Methylation, and Fragmentation of Genomic DNA Genomic DNA was isolated from 5 × 107 HT29 cells with High Pure PCR template preparation kit containing proteinase K (Roche GmbH, Germany).

    Techniques: Expressing, Incubation

    RT-PCR of total RNA of HCECs and human mononuclear cells for COL8A2 and β-actin . Agarose gel shows in lane 1: 1 kb plus ladder, lane 2: COL8A2 with HCECs, lane 3: COL8A2 with HCECs, pretreated with DNase (control to rule out DNA contamination), lane 4: β-actin (housekeeping gene serving as positive control), lane 5: COL8A2 with human mononuclear cells (negative control), lane 6; β-actin with human mononuclear cells (positive control), lane 7: no template (negative control to rule out contamination of reagents), lane 8: PCR with HiFi Taq of HCEC total RNA using COL8A2 primers (negative control to rule out contamination of HCEC total RNA with genomic DNA).

    Journal: Molecular Vision

    Article Title: Comparison of non-viral methods to genetically modify and enrich populations of primary human corneal endothelial cells

    doi:

    Figure Lengend Snippet: RT-PCR of total RNA of HCECs and human mononuclear cells for COL8A2 and β-actin . Agarose gel shows in lane 1: 1 kb plus ladder, lane 2: COL8A2 with HCECs, lane 3: COL8A2 with HCECs, pretreated with DNase (control to rule out DNA contamination), lane 4: β-actin (housekeeping gene serving as positive control), lane 5: COL8A2 with human mononuclear cells (negative control), lane 6; β-actin with human mononuclear cells (positive control), lane 7: no template (negative control to rule out contamination of reagents), lane 8: PCR with HiFi Taq of HCEC total RNA using COL8A2 primers (negative control to rule out contamination of HCEC total RNA with genomic DNA).

    Article Snippet: DNase treatment of RNA samples was performed to rule out genomic DNA contamination following manufacturer’s protocols (Roche Applied Diagnostics, Mannheim, Germany).

    Techniques: Reverse Transcription Polymerase Chain Reaction, Agarose Gel Electrophoresis, Positive Control, Negative Control, Polymerase Chain Reaction

    Mitochondrial DNA genes and gene expression in PD59 cybrid, 10 weeks after a single treatment with MTD–TFAM or MTD–TFAM complexed with mtDNA. Top : mtDNA gene copy numbers normalized to 18S rRNA gene levels for basal conditions and after treatment with MTD–TFAM alone or with MTD–TFAM complexed with human mtDNA. Total genomic DNA was analyzed by qPCR. Bottom .

    Journal: Human Gene Therapy

    Article Title: Mitochondrial Gene Therapy Augments Mitochondrial Physiology in a Parkinson's Disease Cell Model

    doi: 10.1089/hum.2009.023

    Figure Lengend Snippet: Mitochondrial DNA genes and gene expression in PD59 cybrid, 10 weeks after a single treatment with MTD–TFAM or MTD–TFAM complexed with mtDNA. Top : mtDNA gene copy numbers normalized to 18S rRNA gene levels for basal conditions and after treatment with MTD–TFAM alone or with MTD–TFAM complexed with human mtDNA. Total genomic DNA was analyzed by qPCR. Bottom .

    Article Snippet: Human fetal brain cDNA and Roche genomic DNA served as standards for qPCR assays of PGC (peroxisome proliferator-activated receptor [PPAR]-γ-related cofactor)-1α and 18S rRNA gene, respectively.

    Techniques: Expressing, Real-time Polymerase Chain Reaction

    Gel Electrophoresis of PCR Products Lane 1: 100bp DNA ladder Cinagen; Lane 2: negative control; Lane 3, 4 and 5: PCR products for fliC gene (approximately 1500bp) of Salmonella enterica serovar typhimurium ; Lane 6: 1Kbp DNA ladder vivantis.

    Journal: Jundishapur Journal of Microbiology

    Article Title: Cloning of fliC Gene From Salmonella typhimurium in the Expression Vector pVAX1 and Evaluation of its Expression in Eukaryotic Cells

    doi: 10.5812/jjm.12351

    Figure Lengend Snippet: Gel Electrophoresis of PCR Products Lane 1: 100bp DNA ladder Cinagen; Lane 2: negative control; Lane 3, 4 and 5: PCR products for fliC gene (approximately 1500bp) of Salmonella enterica serovar typhimurium ; Lane 6: 1Kbp DNA ladder vivantis.

    Article Snippet: Genomic DNA Extraction Genomic DNA of Salmonella enterica was extracted by High Pure PCR Template Preparation Kit (Roche, Germany), according to the manufacturer’s instruction.

    Techniques: Nucleic Acid Electrophoresis, Polymerase Chain Reaction, Negative Control

    2% agarose gel analysis of PCR mixtures with Lambda phage DNA at 10,000 copies. Each lane contained dATP+dCTP+dGTP (200 μM each). To each reaction 3’-protected dTTP was added at 200 μM final concentration. Lane 1: control (no dTTP or 3’-protected dTTP); lane 2: 3’-Ac dTTP; lane 3: 3’-THP dTTP; lane 4: 3’-MTHP dTTP; lane 5: 3’-THF dTTP; lane 6: 3’-Pac dTTP; lanes 7 and 8: dTTP. Lanes 1-7: with Lambda DNA + HG DNA; lane 8: HG DNA only. At bottom: ratio of amplicon to off-target products estimated by integration of UV-bands.

    Journal: Analytical chemistry

    Article Title: 3'-Protected 2'-Deoxynucleoside 5'-Triphosphates as a Novel Tool for Heat-Triggered Activation of PCR

    doi: 10.1021/ac8026977

    Figure Lengend Snippet: 2% agarose gel analysis of PCR mixtures with Lambda phage DNA at 10,000 copies. Each lane contained dATP+dCTP+dGTP (200 μM each). To each reaction 3’-protected dTTP was added at 200 μM final concentration. Lane 1: control (no dTTP or 3’-protected dTTP); lane 2: 3’-Ac dTTP; lane 3: 3’-THP dTTP; lane 4: 3’-MTHP dTTP; lane 5: 3’-THF dTTP; lane 6: 3’-Pac dTTP; lanes 7 and 8: dTTP. Lanes 1-7: with Lambda DNA + HG DNA; lane 8: HG DNA only. At bottom: ratio of amplicon to off-target products estimated by integration of UV-bands.

    Article Snippet: Bacteriophage lambda genomic DNA was purchased from Roche Applied Science, HIV-1 recombinant genomic DNA was a component of the Gene Amplimer kit purchased from Applied Biosystems, and Human genomic DNA was purchased from Promega.

    Techniques: Agarose Gel Electrophoresis, Polymerase Chain Reaction, Concentration Assay, Lambda DNA Preparation, Amplification

    Lanes: (L) 50-bp DNA ladder; (1), Negative Control for GSTO1; (2), PCR product for GSTO1: 254bp fragment; (3 and 4), homozygote AA: 254 bp fragment; (5), heterozygote AD: 254, 186, and 68-bp fragments; (6), Negative Control for GSTO2; (7), PCR product for GSTO2: 185 bp fragment; (8 and 9), homozygote NN: 185-bp fragment; (10), heterozygote ND: 185, 122 and 63 fragments; (11), homozygote DD: 122 and 63 bp fragments; (L) 50-bp DNA ladder PCR: Polymerase chain reaction

    Journal: Journal of the Turkish German Gynecological Association

    Article Title: Glutathione S-transferase omega gene polymorphism as a biomarker for human papilloma virus and cervical cancer in Iranian women

    doi: 10.4274/jtgga.2018.0056

    Figure Lengend Snippet: Lanes: (L) 50-bp DNA ladder; (1), Negative Control for GSTO1; (2), PCR product for GSTO1: 254bp fragment; (3 and 4), homozygote AA: 254 bp fragment; (5), heterozygote AD: 254, 186, and 68-bp fragments; (6), Negative Control for GSTO2; (7), PCR product for GSTO2: 185 bp fragment; (8 and 9), homozygote NN: 185-bp fragment; (10), heterozygote ND: 185, 122 and 63 fragments; (11), homozygote DD: 122 and 63 bp fragments; (L) 50-bp DNA ladder PCR: Polymerase chain reaction

    Article Snippet: DNA extraction Genomic DNA was extracted using a High Pure PCR Template Preparation Kit (Roche, Germany).

    Techniques: Negative Control, Polymerase Chain Reaction

    Molecular typing and growth curves of serially isolated strains of B. petrii. . (A) The DiversiLab Non fermentor typing kit was used for rep-PCR typing of B. petrii using DNA from clinical and reference strains. Amplicons were detected with the Agilent 2100 bioanalyzer (Agilent Technologies, Palo Alto, CA) and data analyzed with the DiversiLab software (version 3.3). Results generated include a dendrogram (left) and virtual gel images (right). (B) Genomic DNA was digested with the restriction endonuclease XbaI and separated by PFGE with a CHEF Mapper system. Asterisks indicate band differences among the patient strains. Ladder: Lambda DNA Ladder 48.5 KB–1 MB kb plugs (Lonza). (C) Growth curves were performed on LB broth at 37°C and growth assessed by colony-forming units (CFU) performed with serially diluted aliquots plated on SBA plates. Graph shows mean and SEM from three experiments. B. petrii 1–5: strains of B. petrii serially isolated from our patient; BAA-461: type strain of B. petrii (ATCC BAA-461); 13363: first described clinical strain of B. petrii (NCTC 13363).

    Journal: PLoS ONE

    Article Title: Adaptability and Persistence of the Emerging Pathogen Bordetella petrii

    doi: 10.1371/journal.pone.0065102

    Figure Lengend Snippet: Molecular typing and growth curves of serially isolated strains of B. petrii. . (A) The DiversiLab Non fermentor typing kit was used for rep-PCR typing of B. petrii using DNA from clinical and reference strains. Amplicons were detected with the Agilent 2100 bioanalyzer (Agilent Technologies, Palo Alto, CA) and data analyzed with the DiversiLab software (version 3.3). Results generated include a dendrogram (left) and virtual gel images (right). (B) Genomic DNA was digested with the restriction endonuclease XbaI and separated by PFGE with a CHEF Mapper system. Asterisks indicate band differences among the patient strains. Ladder: Lambda DNA Ladder 48.5 KB–1 MB kb plugs (Lonza). (C) Growth curves were performed on LB broth at 37°C and growth assessed by colony-forming units (CFU) performed with serially diluted aliquots plated on SBA plates. Graph shows mean and SEM from three experiments. B. petrii 1–5: strains of B. petrii serially isolated from our patient; BAA-461: type strain of B. petrii (ATCC BAA-461); 13363: first described clinical strain of B. petrii (NCTC 13363).

    Article Snippet: Sequencing Genomic DNA from B. petrii 1, B. petrii 3, B. petrii 4, and B. petrii 5 was sequenced as both fragment and paired end (3 kb-insert) libraries on a 454 FLX pyrosequencer (Roche Branford, CT).

    Techniques: Isolation, Polymerase Chain Reaction, Software, Generated, Lambda DNA Preparation

    Disruption and expression of ERG3 in C. albicans. (A) Southern blot analysis of the ERG3 deletion in C. albicans. Five micrograms of genomic DNA was extracted from each strain, digested with Xba I, separated by electrophoresis, blotted onto a GeneScreen membrane (Dupont NEN), and probed with an Xba I- Pst I fragment from pDS546, as indicated in the schematic restriction map. The following restriction fragment sizes are expected for each hybridization signal: wild-type allele, approximately 23 kb; erg3A Δ:: hisG-URA3-hisG and erg3A Δ:: hisG alleles, 0.9 kb; erg3B Δ:: hisG-URA3-hisG and erg3B Δ:: hisG alleles, 0.5 kb. These sizes correspond to those obtained by Southern blotting for each mutant constructed. (B) ERG3 expression in diverse erg mutants. Total RNA was extracted from C. albicans , and 5 μg of total RNA was separated by electrophoresis and blotted onto GeneScreen membranes. After hybridization with the same probe used for the experiment whose results are shown in panel A and washing steps, the membranes were exposed to Fuji X-ray film and were revealed after exposure to −80°C. The TEF3 -specific probe was hybridized to the same membrane after removal of the ERG3 ).

    Journal: Antimicrobial Agents and Chemotherapy

    Article Title: Candida albicans Mutations in the Ergosterol Biosynthetic Pathway and Resistance to Several Antifungal Agents

    doi: 10.1128/AAC.47.8.2404-2412.2003

    Figure Lengend Snippet: Disruption and expression of ERG3 in C. albicans. (A) Southern blot analysis of the ERG3 deletion in C. albicans. Five micrograms of genomic DNA was extracted from each strain, digested with Xba I, separated by electrophoresis, blotted onto a GeneScreen membrane (Dupont NEN), and probed with an Xba I- Pst I fragment from pDS546, as indicated in the schematic restriction map. The following restriction fragment sizes are expected for each hybridization signal: wild-type allele, approximately 23 kb; erg3A Δ:: hisG-URA3-hisG and erg3A Δ:: hisG alleles, 0.9 kb; erg3B Δ:: hisG-URA3-hisG and erg3B Δ:: hisG alleles, 0.5 kb. These sizes correspond to those obtained by Southern blotting for each mutant constructed. (B) ERG3 expression in diverse erg mutants. Total RNA was extracted from C. albicans , and 5 μg of total RNA was separated by electrophoresis and blotted onto GeneScreen membranes. After hybridization with the same probe used for the experiment whose results are shown in panel A and washing steps, the membranes were exposed to Fuji X-ray film and were revealed after exposure to −80°C. The TEF3 -specific probe was hybridized to the same membrane after removal of the ERG3 ).

    Article Snippet: For the second ERG3 disruption cassette, a fragment corresponding to the second ERG3 allele ( ERG3B ) was amplified from C. albicans genomic DNA with primers ERG3-XBA (5′-TACAATCTAGATATCTTTGGACATTCT-3′) and ERG3-XHO (5′-GCGCAAACTCGAGAAGATTATTTTCAA TTATCAACACCAAATTG-3′) and cloned into a compatible restriction site of pBluescript KS(+) to yield pDS749.

    Techniques: Expressing, Southern Blot, Electrophoresis, Hybridization, Mutagenesis, Construct

    (A) Methylation‐specific melting curve analysis for the IMiD‐sensitive and IMiD‐resistant cell lines, showing the absence of promoter DNA methylation of CRBN. The blue curves are the fully unmethylated and fully methylated controls (left and right curve, respectively), and the green lines represent the cell samples. (B, C) ChIP for H3K4me3, H3K27Ac, and H3K27me3 around the transcription site of CRBN for OPM2 and NCI‐H929, respectively, revealed that IMiD‐resistant cell lines retained their activating histone marks (H3K4me3, H3K27Ac) without any significant increase in the repressive mark H3K27me3. (D) Chromatin accessibility values for the probes mapping to CRBN, assessed with Acce SssI ble. The difference in the accessibility of the resistant cell lines compared with their parental cell line (ΔAcc) was calculated by subtracting the accessibility value of the paternal cell line from the accessibility value of the resistant cell line for each individual probe. None of the probes covering CRBN showed significant accessibility changes in any of the IMiD‐resistant cell lines. (E, F) Kernel density plot of the combined DNA methylation changes and chromatin accessibility changes observed in the IMiD‐resistant cell lines. The OPM2 IMiD‐resistant cells exhibit primarily a decrease in the global chromatin accessibility when compared with the parental OPM2 (E), while H929 IMiD‐resistant cell lines exhibit a pattern of combined loss of chromatin accessibility with increased DNA methylation (F).

    Journal: Molecular Oncology

    Article Title: Dual inhibition of DNMTs and EZH2 can overcome both intrinsic and acquired resistance of myeloma cells to IMiDs in a cereblon‐independent manner

    doi: 10.1002/1878-0261.12157

    Figure Lengend Snippet: (A) Methylation‐specific melting curve analysis for the IMiD‐sensitive and IMiD‐resistant cell lines, showing the absence of promoter DNA methylation of CRBN. The blue curves are the fully unmethylated and fully methylated controls (left and right curve, respectively), and the green lines represent the cell samples. (B, C) ChIP for H3K4me3, H3K27Ac, and H3K27me3 around the transcription site of CRBN for OPM2 and NCI‐H929, respectively, revealed that IMiD‐resistant cell lines retained their activating histone marks (H3K4me3, H3K27Ac) without any significant increase in the repressive mark H3K27me3. (D) Chromatin accessibility values for the probes mapping to CRBN, assessed with Acce SssI ble. The difference in the accessibility of the resistant cell lines compared with their parental cell line (ΔAcc) was calculated by subtracting the accessibility value of the paternal cell line from the accessibility value of the resistant cell line for each individual probe. None of the probes covering CRBN showed significant accessibility changes in any of the IMiD‐resistant cell lines. (E, F) Kernel density plot of the combined DNA methylation changes and chromatin accessibility changes observed in the IMiD‐resistant cell lines. The OPM2 IMiD‐resistant cells exhibit primarily a decrease in the global chromatin accessibility when compared with the parental OPM2 (E), while H929 IMiD‐resistant cell lines exhibit a pattern of combined loss of chromatin accessibility with increased DNA methylation (F).

    Article Snippet: SssI‐treated DNA (Millipore, Burlington, MA, USA) and unmethylated genomic DNA (Roche) were also bisulfite‐converted and used as positive (methylated) and negative (unmethylated) control, respectively.

    Techniques: Methylation, DNA Methylation Assay, Chromatin Immunoprecipitation

    Real-time PCR detection of the amplification of a 533-bp fragment of the DNA packaging protein of enterobacteria phage λ using TaqMan® probe detection. Reactions were performed in quadruplicate and compared the performance of unmodified and double OXP-modified primers for their ability to detect 10, 50, 100, 500, 1000, 5000 and 10 000 copies of λ genomic DNA. This figure displays the amplification plots for unmodified ( A ) and double OXP-modified ( B ) primers as well as the corresponding standard curve ( C ).

    Journal: Nucleic Acids Research

    Article Title: Hot Start PCR with heat-activatable primers: a novel approach for improved PCR performance

    doi: 10.1093/nar/gkn575

    Figure Lengend Snippet: Real-time PCR detection of the amplification of a 533-bp fragment of the DNA packaging protein of enterobacteria phage λ using TaqMan® probe detection. Reactions were performed in quadruplicate and compared the performance of unmodified and double OXP-modified primers for their ability to detect 10, 50, 100, 500, 1000, 5000 and 10 000 copies of λ genomic DNA. This figure displays the amplification plots for unmodified ( A ) and double OXP-modified ( B ) primers as well as the corresponding standard curve ( C ).

    Article Snippet: Bacteriophage λ genomic DNA was purchased from Roche Applied Science (Indianapolis, IN, USA), HIV-1 genomic DNA was a component of the Gene Amplimer kit purchased from Applied Biosystems (Foster City, CA, USA), and Human genomic DNA was purchased from Promega (Fitchburg, WI, USA).

    Techniques: Real-time Polymerase Chain Reaction, Amplification, Modification