genomic dna Qiagen Search Results


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  • 95
    Qiagen 100 g genomic tip system
    100 G Genomic Tip System, supplied by Qiagen, used in various techniques. Bioz Stars score: 95/100, based on 10 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/100 g genomic tip system/product/Qiagen
    Average 95 stars, based on 10 article reviews
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    100 g genomic tip system - by Bioz Stars, 2020-02
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    95
    Millipore gdna
    Gdna, supplied by Millipore, used in various techniques. Bioz Stars score: 95/100, based on 714 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    Average 95 stars, based on 714 article reviews
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    gdna - by Bioz Stars, 2020-02
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    79
    Qiagen genomic dna gdna eliminator columns
    HDI do not alter <t>DNA</t> methylation and histone acetylation in the Aicda promoter. ( A and B ) CpG DNA methylation of the Aicda promoter was analyzed by bisulfite sequencing of genomic DNA from B cells stimulated for 4 d with LPS plus IL-4 in the presence of increasing doses of VPA. ( A ) DNA sequencing of PCR products of bisulfite-treated genomic DNA. The sequence signal from dCs in CpG motifs is framed. As unmethylated dC nucleotides can be converted to dU (read as dT in DNA sequence), while methylated dC cannot, the ratio of dC (blue) to dT (red) signal indicates the level of methylated dC at any given positions. ( B ) Methylation pattern at each of the four dCs within CpG motifs from individually cloned sequences (each row is a unique sequence and each dC is represented by a column of circles) is shown as an array of circles (closed circles represent methylated dCs; open circles represent unmethylated dCs). ( C ) Abundance of acetylated histone H3 (H3K9ac/K14ac) in the Aicda promoter in B cells stimulated with LPS plus IL-4 for 60 h in the presence of nil or VPA (1,000 μM) was measured by ChIP and qPCR. ( D ) Primary (pri-) miRNA transcripts of miR-155 and miR-181b in B cells cultured for 60 h with LPS or LPS plus IL-4 in the presence of nil or increasing doses of VPA, were measured by qRT-PCR and normalized to Cd79b expression. Values in B cells cultured in medium containing VPA are depicted as relative to the values in B cells cultured in the absence of HDI, set as 1. Data are presented as mean and SEM from three independent experiments. * p
    Genomic Dna Gdna Eliminator Columns, supplied by Qiagen, used in various techniques. Bioz Stars score: 79/100, based on 37 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    Average 79 stars, based on 37 article reviews
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    genomic dna gdna eliminator columns - by Bioz Stars, 2020-02
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    gdna  (Qiagen)
    99
    Qiagen gdna
    HDI do not alter <t>DNA</t> methylation and histone acetylation in the Aicda promoter. ( A and B ) CpG DNA methylation of the Aicda promoter was analyzed by bisulfite sequencing of genomic DNA from B cells stimulated for 4 d with LPS plus IL-4 in the presence of increasing doses of VPA. ( A ) DNA sequencing of PCR products of bisulfite-treated genomic DNA. The sequence signal from dCs in CpG motifs is framed. As unmethylated dC nucleotides can be converted to dU (read as dT in DNA sequence), while methylated dC cannot, the ratio of dC (blue) to dT (red) signal indicates the level of methylated dC at any given positions. ( B ) Methylation pattern at each of the four dCs within CpG motifs from individually cloned sequences (each row is a unique sequence and each dC is represented by a column of circles) is shown as an array of circles (closed circles represent methylated dCs; open circles represent unmethylated dCs). ( C ) Abundance of acetylated histone H3 (H3K9ac/K14ac) in the Aicda promoter in B cells stimulated with LPS plus IL-4 for 60 h in the presence of nil or VPA (1,000 μM) was measured by ChIP and qPCR. ( D ) Primary (pri-) miRNA transcripts of miR-155 and miR-181b in B cells cultured for 60 h with LPS or LPS plus IL-4 in the presence of nil or increasing doses of VPA, were measured by qRT-PCR and normalized to Cd79b expression. Values in B cells cultured in medium containing VPA are depicted as relative to the values in B cells cultured in the absence of HDI, set as 1. Data are presented as mean and SEM from three independent experiments. * p
    Gdna, supplied by Qiagen, used in various techniques. Bioz Stars score: 99/100, based on 4340 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/gdna/product/Qiagen
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    gdna - by Bioz Stars, 2020-02
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    79
    Qiagen gdna kit
    HDI do not alter <t>DNA</t> methylation and histone acetylation in the Aicda promoter. ( A and B ) CpG DNA methylation of the Aicda promoter was analyzed by bisulfite sequencing of genomic DNA from B cells stimulated for 4 d with LPS plus IL-4 in the presence of increasing doses of VPA. ( A ) DNA sequencing of PCR products of bisulfite-treated genomic DNA. The sequence signal from dCs in CpG motifs is framed. As unmethylated dC nucleotides can be converted to dU (read as dT in DNA sequence), while methylated dC cannot, the ratio of dC (blue) to dT (red) signal indicates the level of methylated dC at any given positions. ( B ) Methylation pattern at each of the four dCs within CpG motifs from individually cloned sequences (each row is a unique sequence and each dC is represented by a column of circles) is shown as an array of circles (closed circles represent methylated dCs; open circles represent unmethylated dCs). ( C ) Abundance of acetylated histone H3 (H3K9ac/K14ac) in the Aicda promoter in B cells stimulated with LPS plus IL-4 for 60 h in the presence of nil or VPA (1,000 μM) was measured by ChIP and qPCR. ( D ) Primary (pri-) miRNA transcripts of miR-155 and miR-181b in B cells cultured for 60 h with LPS or LPS plus IL-4 in the presence of nil or increasing doses of VPA, were measured by qRT-PCR and normalized to Cd79b expression. Values in B cells cultured in medium containing VPA are depicted as relative to the values in B cells cultured in the absence of HDI, set as 1. Data are presented as mean and SEM from three independent experiments. * p
    Gdna Kit, supplied by Qiagen, used in various techniques. Bioz Stars score: 79/100, based on 9 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    Average 79 stars, based on 9 article reviews
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    90
    Thermo Fisher easy dnatm gdna purification kit
    HDI do not alter <t>DNA</t> methylation and histone acetylation in the Aicda promoter. ( A and B ) CpG DNA methylation of the Aicda promoter was analyzed by bisulfite sequencing of genomic DNA from B cells stimulated for 4 d with LPS plus IL-4 in the presence of increasing doses of VPA. ( A ) DNA sequencing of PCR products of bisulfite-treated genomic DNA. The sequence signal from dCs in CpG motifs is framed. As unmethylated dC nucleotides can be converted to dU (read as dT in DNA sequence), while methylated dC cannot, the ratio of dC (blue) to dT (red) signal indicates the level of methylated dC at any given positions. ( B ) Methylation pattern at each of the four dCs within CpG motifs from individually cloned sequences (each row is a unique sequence and each dC is represented by a column of circles) is shown as an array of circles (closed circles represent methylated dCs; open circles represent unmethylated dCs). ( C ) Abundance of acetylated histone H3 (H3K9ac/K14ac) in the Aicda promoter in B cells stimulated with LPS plus IL-4 for 60 h in the presence of nil or VPA (1,000 μM) was measured by ChIP and qPCR. ( D ) Primary (pri-) miRNA transcripts of miR-155 and miR-181b in B cells cultured for 60 h with LPS or LPS plus IL-4 in the presence of nil or increasing doses of VPA, were measured by qRT-PCR and normalized to Cd79b expression. Values in B cells cultured in medium containing VPA are depicted as relative to the values in B cells cultured in the absence of HDI, set as 1. Data are presented as mean and SEM from three independent experiments. * p
    Easy Dnatm Gdna Purification Kit, supplied by Thermo Fisher, used in various techniques. Bioz Stars score: 90/100, based on 14 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    Average 90 stars, based on 14 article reviews
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    easy dnatm gdna purification kit - by Bioz Stars, 2020-02
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    85
    Qiagen rhesus macaque genomic dna gdna contamination
    HDI do not alter <t>DNA</t> methylation and histone acetylation in the Aicda promoter. ( A and B ) CpG DNA methylation of the Aicda promoter was analyzed by bisulfite sequencing of genomic DNA from B cells stimulated for 4 d with LPS plus IL-4 in the presence of increasing doses of VPA. ( A ) DNA sequencing of PCR products of bisulfite-treated genomic DNA. The sequence signal from dCs in CpG motifs is framed. As unmethylated dC nucleotides can be converted to dU (read as dT in DNA sequence), while methylated dC cannot, the ratio of dC (blue) to dT (red) signal indicates the level of methylated dC at any given positions. ( B ) Methylation pattern at each of the four dCs within CpG motifs from individually cloned sequences (each row is a unique sequence and each dC is represented by a column of circles) is shown as an array of circles (closed circles represent methylated dCs; open circles represent unmethylated dCs). ( C ) Abundance of acetylated histone H3 (H3K9ac/K14ac) in the Aicda promoter in B cells stimulated with LPS plus IL-4 for 60 h in the presence of nil or VPA (1,000 μM) was measured by ChIP and qPCR. ( D ) Primary (pri-) miRNA transcripts of miR-155 and miR-181b in B cells cultured for 60 h with LPS or LPS plus IL-4 in the presence of nil or increasing doses of VPA, were measured by qRT-PCR and normalized to Cd79b expression. Values in B cells cultured in medium containing VPA are depicted as relative to the values in B cells cultured in the absence of HDI, set as 1. Data are presented as mean and SEM from three independent experiments. * p
    Rhesus Macaque Genomic Dna Gdna Contamination, supplied by Qiagen, used in various techniques. Bioz Stars score: 85/100, based on 9 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/rhesus macaque genomic dna gdna contamination/product/Qiagen
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    99
    Qiagen gdna wipeout buffer
    HDI do not alter <t>DNA</t> methylation and histone acetylation in the Aicda promoter. ( A and B ) CpG DNA methylation of the Aicda promoter was analyzed by bisulfite sequencing of genomic DNA from B cells stimulated for 4 d with LPS plus IL-4 in the presence of increasing doses of VPA. ( A ) DNA sequencing of PCR products of bisulfite-treated genomic DNA. The sequence signal from dCs in CpG motifs is framed. As unmethylated dC nucleotides can be converted to dU (read as dT in DNA sequence), while methylated dC cannot, the ratio of dC (blue) to dT (red) signal indicates the level of methylated dC at any given positions. ( B ) Methylation pattern at each of the four dCs within CpG motifs from individually cloned sequences (each row is a unique sequence and each dC is represented by a column of circles) is shown as an array of circles (closed circles represent methylated dCs; open circles represent unmethylated dCs). ( C ) Abundance of acetylated histone H3 (H3K9ac/K14ac) in the Aicda promoter in B cells stimulated with LPS plus IL-4 for 60 h in the presence of nil or VPA (1,000 μM) was measured by ChIP and qPCR. ( D ) Primary (pri-) miRNA transcripts of miR-155 and miR-181b in B cells cultured for 60 h with LPS or LPS plus IL-4 in the presence of nil or increasing doses of VPA, were measured by qRT-PCR and normalized to Cd79b expression. Values in B cells cultured in medium containing VPA are depicted as relative to the values in B cells cultured in the absence of HDI, set as 1. Data are presented as mean and SEM from three independent experiments. * p
    Gdna Wipeout Buffer, supplied by Qiagen, used in various techniques. Bioz Stars score: 99/100, based on 166 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    78
    Qiagen qiaquick gdna columns
    HDI do not alter <t>DNA</t> methylation and histone acetylation in the Aicda promoter. ( A and B ) CpG DNA methylation of the Aicda promoter was analyzed by bisulfite sequencing of genomic DNA from B cells stimulated for 4 d with LPS plus IL-4 in the presence of increasing doses of VPA. ( A ) DNA sequencing of PCR products of bisulfite-treated genomic DNA. The sequence signal from dCs in CpG motifs is framed. As unmethylated dC nucleotides can be converted to dU (read as dT in DNA sequence), while methylated dC cannot, the ratio of dC (blue) to dT (red) signal indicates the level of methylated dC at any given positions. ( B ) Methylation pattern at each of the four dCs within CpG motifs from individually cloned sequences (each row is a unique sequence and each dC is represented by a column of circles) is shown as an array of circles (closed circles represent methylated dCs; open circles represent unmethylated dCs). ( C ) Abundance of acetylated histone H3 (H3K9ac/K14ac) in the Aicda promoter in B cells stimulated with LPS plus IL-4 for 60 h in the presence of nil or VPA (1,000 μM) was measured by ChIP and qPCR. ( D ) Primary (pri-) miRNA transcripts of miR-155 and miR-181b in B cells cultured for 60 h with LPS or LPS plus IL-4 in the presence of nil or increasing doses of VPA, were measured by qRT-PCR and normalized to Cd79b expression. Values in B cells cultured in medium containing VPA are depicted as relative to the values in B cells cultured in the absence of HDI, set as 1. Data are presented as mean and SEM from three independent experiments. * p
    Qiaquick Gdna Columns, supplied by Qiagen, used in various techniques. Bioz Stars score: 78/100, based on 7 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    Average 78 stars, based on 7 article reviews
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    80
    Qiagen gdna filter column
    HDI do not alter <t>DNA</t> methylation and histone acetylation in the Aicda promoter. ( A and B ) CpG DNA methylation of the Aicda promoter was analyzed by bisulfite sequencing of genomic DNA from B cells stimulated for 4 d with LPS plus IL-4 in the presence of increasing doses of VPA. ( A ) DNA sequencing of PCR products of bisulfite-treated genomic DNA. The sequence signal from dCs in CpG motifs is framed. As unmethylated dC nucleotides can be converted to dU (read as dT in DNA sequence), while methylated dC cannot, the ratio of dC (blue) to dT (red) signal indicates the level of methylated dC at any given positions. ( B ) Methylation pattern at each of the four dCs within CpG motifs from individually cloned sequences (each row is a unique sequence and each dC is represented by a column of circles) is shown as an array of circles (closed circles represent methylated dCs; open circles represent unmethylated dCs). ( C ) Abundance of acetylated histone H3 (H3K9ac/K14ac) in the Aicda promoter in B cells stimulated with LPS plus IL-4 for 60 h in the presence of nil or VPA (1,000 μM) was measured by ChIP and qPCR. ( D ) Primary (pri-) miRNA transcripts of miR-155 and miR-181b in B cells cultured for 60 h with LPS or LPS plus IL-4 in the presence of nil or increasing doses of VPA, were measured by qRT-PCR and normalized to Cd79b expression. Values in B cells cultured in medium containing VPA are depicted as relative to the values in B cells cultured in the absence of HDI, set as 1. Data are presented as mean and SEM from three independent experiments. * p
    Gdna Filter Column, supplied by Qiagen, used in various techniques. Bioz Stars score: 80/100, based on 9 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    77
    Qiagen gdna wipeout reagent
    HDI do not alter <t>DNA</t> methylation and histone acetylation in the Aicda promoter. ( A and B ) CpG DNA methylation of the Aicda promoter was analyzed by bisulfite sequencing of genomic DNA from B cells stimulated for 4 d with LPS plus IL-4 in the presence of increasing doses of VPA. ( A ) DNA sequencing of PCR products of bisulfite-treated genomic DNA. The sequence signal from dCs in CpG motifs is framed. As unmethylated dC nucleotides can be converted to dU (read as dT in DNA sequence), while methylated dC cannot, the ratio of dC (blue) to dT (red) signal indicates the level of methylated dC at any given positions. ( B ) Methylation pattern at each of the four dCs within CpG motifs from individually cloned sequences (each row is a unique sequence and each dC is represented by a column of circles) is shown as an array of circles (closed circles represent methylated dCs; open circles represent unmethylated dCs). ( C ) Abundance of acetylated histone H3 (H3K9ac/K14ac) in the Aicda promoter in B cells stimulated with LPS plus IL-4 for 60 h in the presence of nil or VPA (1,000 μM) was measured by ChIP and qPCR. ( D ) Primary (pri-) miRNA transcripts of miR-155 and miR-181b in B cells cultured for 60 h with LPS or LPS plus IL-4 in the presence of nil or increasing doses of VPA, were measured by qRT-PCR and normalized to Cd79b expression. Values in B cells cultured in medium containing VPA are depicted as relative to the values in B cells cultured in the absence of HDI, set as 1. Data are presented as mean and SEM from three independent experiments. * p
    Gdna Wipeout Reagent, supplied by Qiagen, used in various techniques. Bioz Stars score: 77/100, based on 5 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    78
    Qiagen gdna eliminator system
    HDI do not alter <t>DNA</t> methylation and histone acetylation in the Aicda promoter. ( A and B ) CpG DNA methylation of the Aicda promoter was analyzed by bisulfite sequencing of genomic DNA from B cells stimulated for 4 d with LPS plus IL-4 in the presence of increasing doses of VPA. ( A ) DNA sequencing of PCR products of bisulfite-treated genomic DNA. The sequence signal from dCs in CpG motifs is framed. As unmethylated dC nucleotides can be converted to dU (read as dT in DNA sequence), while methylated dC cannot, the ratio of dC (blue) to dT (red) signal indicates the level of methylated dC at any given positions. ( B ) Methylation pattern at each of the four dCs within CpG motifs from individually cloned sequences (each row is a unique sequence and each dC is represented by a column of circles) is shown as an array of circles (closed circles represent methylated dCs; open circles represent unmethylated dCs). ( C ) Abundance of acetylated histone H3 (H3K9ac/K14ac) in the Aicda promoter in B cells stimulated with LPS plus IL-4 for 60 h in the presence of nil or VPA (1,000 μM) was measured by ChIP and qPCR. ( D ) Primary (pri-) miRNA transcripts of miR-155 and miR-181b in B cells cultured for 60 h with LPS or LPS plus IL-4 in the presence of nil or increasing doses of VPA, were measured by qRT-PCR and normalized to Cd79b expression. Values in B cells cultured in medium containing VPA are depicted as relative to the values in B cells cultured in the absence of HDI, set as 1. Data are presented as mean and SEM from three independent experiments. * p
    Gdna Eliminator System, supplied by Qiagen, used in various techniques. Bioz Stars score: 78/100, based on 8 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    78
    Qiagen gdna whipeout buffer
    HDI do not alter <t>DNA</t> methylation and histone acetylation in the Aicda promoter. ( A and B ) CpG DNA methylation of the Aicda promoter was analyzed by bisulfite sequencing of genomic DNA from B cells stimulated for 4 d with LPS plus IL-4 in the presence of increasing doses of VPA. ( A ) DNA sequencing of PCR products of bisulfite-treated genomic DNA. The sequence signal from dCs in CpG motifs is framed. As unmethylated dC nucleotides can be converted to dU (read as dT in DNA sequence), while methylated dC cannot, the ratio of dC (blue) to dT (red) signal indicates the level of methylated dC at any given positions. ( B ) Methylation pattern at each of the four dCs within CpG motifs from individually cloned sequences (each row is a unique sequence and each dC is represented by a column of circles) is shown as an array of circles (closed circles represent methylated dCs; open circles represent unmethylated dCs). ( C ) Abundance of acetylated histone H3 (H3K9ac/K14ac) in the Aicda promoter in B cells stimulated with LPS plus IL-4 for 60 h in the presence of nil or VPA (1,000 μM) was measured by ChIP and qPCR. ( D ) Primary (pri-) miRNA transcripts of miR-155 and miR-181b in B cells cultured for 60 h with LPS or LPS plus IL-4 in the presence of nil or increasing doses of VPA, were measured by qRT-PCR and normalized to Cd79b expression. Values in B cells cultured in medium containing VPA are depicted as relative to the values in B cells cultured in the absence of HDI, set as 1. Data are presented as mean and SEM from three independent experiments. * p
    Gdna Whipeout Buffer, supplied by Qiagen, used in various techniques. Bioz Stars score: 78/100, based on 6 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/gdna whipeout buffer/product/Qiagen
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    79
    Qiagen gdna amplified fragment
    HDI do not alter <t>DNA</t> methylation and histone acetylation in the Aicda promoter. ( A and B ) CpG DNA methylation of the Aicda promoter was analyzed by bisulfite sequencing of genomic DNA from B cells stimulated for 4 d with LPS plus IL-4 in the presence of increasing doses of VPA. ( A ) DNA sequencing of PCR products of bisulfite-treated genomic DNA. The sequence signal from dCs in CpG motifs is framed. As unmethylated dC nucleotides can be converted to dU (read as dT in DNA sequence), while methylated dC cannot, the ratio of dC (blue) to dT (red) signal indicates the level of methylated dC at any given positions. ( B ) Methylation pattern at each of the four dCs within CpG motifs from individually cloned sequences (each row is a unique sequence and each dC is represented by a column of circles) is shown as an array of circles (closed circles represent methylated dCs; open circles represent unmethylated dCs). ( C ) Abundance of acetylated histone H3 (H3K9ac/K14ac) in the Aicda promoter in B cells stimulated with LPS plus IL-4 for 60 h in the presence of nil or VPA (1,000 μM) was measured by ChIP and qPCR. ( D ) Primary (pri-) miRNA transcripts of miR-155 and miR-181b in B cells cultured for 60 h with LPS or LPS plus IL-4 in the presence of nil or increasing doses of VPA, were measured by qRT-PCR and normalized to Cd79b expression. Values in B cells cultured in medium containing VPA are depicted as relative to the values in B cells cultured in the absence of HDI, set as 1. Data are presented as mean and SEM from three independent experiments. * p
    Gdna Amplified Fragment, supplied by Qiagen, used in various techniques. Bioz Stars score: 79/100, based on 10 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    78
    Qiagen gdna elimination step
    HDI do not alter <t>DNA</t> methylation and histone acetylation in the Aicda promoter. ( A and B ) CpG DNA methylation of the Aicda promoter was analyzed by bisulfite sequencing of genomic DNA from B cells stimulated for 4 d with LPS plus IL-4 in the presence of increasing doses of VPA. ( A ) DNA sequencing of PCR products of bisulfite-treated genomic DNA. The sequence signal from dCs in CpG motifs is framed. As unmethylated dC nucleotides can be converted to dU (read as dT in DNA sequence), while methylated dC cannot, the ratio of dC (blue) to dT (red) signal indicates the level of methylated dC at any given positions. ( B ) Methylation pattern at each of the four dCs within CpG motifs from individually cloned sequences (each row is a unique sequence and each dC is represented by a column of circles) is shown as an array of circles (closed circles represent methylated dCs; open circles represent unmethylated dCs). ( C ) Abundance of acetylated histone H3 (H3K9ac/K14ac) in the Aicda promoter in B cells stimulated with LPS plus IL-4 for 60 h in the presence of nil or VPA (1,000 μM) was measured by ChIP and qPCR. ( D ) Primary (pri-) miRNA transcripts of miR-155 and miR-181b in B cells cultured for 60 h with LPS or LPS plus IL-4 in the presence of nil or increasing doses of VPA, were measured by qRT-PCR and normalized to Cd79b expression. Values in B cells cultured in medium containing VPA are depicted as relative to the values in B cells cultured in the absence of HDI, set as 1. Data are presented as mean and SEM from three independent experiments. * p
    Gdna Elimination Step, supplied by Qiagen, used in various techniques. Bioz Stars score: 78/100, based on 5 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    77
    Zymo Research quick gdna prep
    HDI do not alter <t>DNA</t> methylation and histone acetylation in the Aicda promoter. ( A and B ) CpG DNA methylation of the Aicda promoter was analyzed by bisulfite sequencing of genomic DNA from B cells stimulated for 4 d with LPS plus IL-4 in the presence of increasing doses of VPA. ( A ) DNA sequencing of PCR products of bisulfite-treated genomic DNA. The sequence signal from dCs in CpG motifs is framed. As unmethylated dC nucleotides can be converted to dU (read as dT in DNA sequence), while methylated dC cannot, the ratio of dC (blue) to dT (red) signal indicates the level of methylated dC at any given positions. ( B ) Methylation pattern at each of the four dCs within CpG motifs from individually cloned sequences (each row is a unique sequence and each dC is represented by a column of circles) is shown as an array of circles (closed circles represent methylated dCs; open circles represent unmethylated dCs). ( C ) Abundance of acetylated histone H3 (H3K9ac/K14ac) in the Aicda promoter in B cells stimulated with LPS plus IL-4 for 60 h in the presence of nil or VPA (1,000 μM) was measured by ChIP and qPCR. ( D ) Primary (pri-) miRNA transcripts of miR-155 and miR-181b in B cells cultured for 60 h with LPS or LPS plus IL-4 in the presence of nil or increasing doses of VPA, were measured by qRT-PCR and normalized to Cd79b expression. Values in B cells cultured in medium containing VPA are depicted as relative to the values in B cells cultured in the absence of HDI, set as 1. Data are presented as mean and SEM from three independent experiments. * p
    Quick Gdna Prep, supplied by Zymo Research, used in various techniques. Bioz Stars score: 77/100, based on 4 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    87
    Qiagen gdna purification kit
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    Image Search Results


    HDI do not alter DNA methylation and histone acetylation in the Aicda promoter. ( A and B ) CpG DNA methylation of the Aicda promoter was analyzed by bisulfite sequencing of genomic DNA from B cells stimulated for 4 d with LPS plus IL-4 in the presence of increasing doses of VPA. ( A ) DNA sequencing of PCR products of bisulfite-treated genomic DNA. The sequence signal from dCs in CpG motifs is framed. As unmethylated dC nucleotides can be converted to dU (read as dT in DNA sequence), while methylated dC cannot, the ratio of dC (blue) to dT (red) signal indicates the level of methylated dC at any given positions. ( B ) Methylation pattern at each of the four dCs within CpG motifs from individually cloned sequences (each row is a unique sequence and each dC is represented by a column of circles) is shown as an array of circles (closed circles represent methylated dCs; open circles represent unmethylated dCs). ( C ) Abundance of acetylated histone H3 (H3K9ac/K14ac) in the Aicda promoter in B cells stimulated with LPS plus IL-4 for 60 h in the presence of nil or VPA (1,000 μM) was measured by ChIP and qPCR. ( D ) Primary (pri-) miRNA transcripts of miR-155 and miR-181b in B cells cultured for 60 h with LPS or LPS plus IL-4 in the presence of nil or increasing doses of VPA, were measured by qRT-PCR and normalized to Cd79b expression. Values in B cells cultured in medium containing VPA are depicted as relative to the values in B cells cultured in the absence of HDI, set as 1. Data are presented as mean and SEM from three independent experiments. * p

    Journal: Journal of immunology (Baltimore, Md. : 1950)

    Article Title: HDAC Inhibitors Upregulate B Cell microRNAs that Silence AID and Blimp-1 Expression for Epigenetic Modulation of Antibody and Autoantibody Responses

    doi: 10.4049/jimmunol.1401702

    Figure Lengend Snippet: HDI do not alter DNA methylation and histone acetylation in the Aicda promoter. ( A and B ) CpG DNA methylation of the Aicda promoter was analyzed by bisulfite sequencing of genomic DNA from B cells stimulated for 4 d with LPS plus IL-4 in the presence of increasing doses of VPA. ( A ) DNA sequencing of PCR products of bisulfite-treated genomic DNA. The sequence signal from dCs in CpG motifs is framed. As unmethylated dC nucleotides can be converted to dU (read as dT in DNA sequence), while methylated dC cannot, the ratio of dC (blue) to dT (red) signal indicates the level of methylated dC at any given positions. ( B ) Methylation pattern at each of the four dCs within CpG motifs from individually cloned sequences (each row is a unique sequence and each dC is represented by a column of circles) is shown as an array of circles (closed circles represent methylated dCs; open circles represent unmethylated dCs). ( C ) Abundance of acetylated histone H3 (H3K9ac/K14ac) in the Aicda promoter in B cells stimulated with LPS plus IL-4 for 60 h in the presence of nil or VPA (1,000 μM) was measured by ChIP and qPCR. ( D ) Primary (pri-) miRNA transcripts of miR-155 and miR-181b in B cells cultured for 60 h with LPS or LPS plus IL-4 in the presence of nil or increasing doses of VPA, were measured by qRT-PCR and normalized to Cd79b expression. Values in B cells cultured in medium containing VPA are depicted as relative to the values in B cells cultured in the absence of HDI, set as 1. Data are presented as mean and SEM from three independent experiments. * p

    Article Snippet: Residual DNA was removed from the extracted RNA with gDNA eliminator columns (Qiagen). cDNA was synthesized from total RNA with the SuperScript™ III First-Strand Synthesis System (Invitrogen) using oligo-dT primer.

    Techniques: DNA Methylation Assay, Methylation Sequencing, DNA Sequencing, Polymerase Chain Reaction, Sequencing, Methylation, Clone Assay, Chromatin Immunoprecipitation, Real-time Polymerase Chain Reaction, Cell Culture, Quantitative RT-PCR, Expressing

    Circular genome map of S. algae ACCC. Circles from the outside to inside showing: (1) DNA coordinates; (2, 3) function-based color coded mapping of the CDSs predicted on the forward and reverse strands. Functions are color-code; (4) tRNA genes; (5) rRNA genes; (6) GC plot showing regions above the average (green) and below (violet); (7) GC skew showing regions above average (yellow) and below (light blue)

    Journal: Gut Pathogens

    Article Title: Genome characterization of bile-isolated Shewanella algae ACCC

    doi: 10.1186/s13099-018-0267-4

    Figure Lengend Snippet: Circular genome map of S. algae ACCC. Circles from the outside to inside showing: (1) DNA coordinates; (2, 3) function-based color coded mapping of the CDSs predicted on the forward and reverse strands. Functions are color-code; (4) tRNA genes; (5) rRNA genes; (6) GC plot showing regions above the average (green) and below (violet); (7) GC skew showing regions above average (yellow) and below (light blue)

    Article Snippet: Library preparation, whole-genome sequence archive, and de novo assembly The bacterial genomic DNA was extracted from overnight culture of the S. algae ACCC using the QIAGEN Genomic-tip 100/G kit and Genomic DNA Buffer Set (QIAGEN, Valencia, CA) according to the manufacturer’s instructions.

    Techniques: