geneticin selective antibiotic Search Results


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  • 98
    Thermo Fisher g 418 antibiotic selection
    Sperm of Ppatg5 lines are infertile whereas the Ppatg5 egg upon fertilization, can develop into a functional sporophyte that produces viable spores. (A) differential interference contrast micrographs of representative gametophore apices 40 d after induction of reproductive development. Self-fertilization of Ppatg5 lines always fails. The red mark highlights the difference in size between a nonfertilized archegonium and a young sporophyte from a successful fertilization. Sporophyte maturation after successful female Ppatg5 x male WT crosses progresses much slower than after self-fertilization of WT. Scale bar: 200 µm (B) Sporophyte development comparison when WT is fully mature and has already burst. Scale bar: 1 mm. (C) Germinated spores in the presence or absence of selection marker <t>(geneticin),</t> which selects for genotypes carrying the Ppatg5 KO construct. (D) RT-PCR confirmation of colony genotype using primers p5 and p6. For principal positions of annealing sites and sequence of primers, see Figure S2A and Table S1.
    G 418 Antibiotic Selection, supplied by Thermo Fisher, used in various techniques. Bioz Stars score: 98/100, based on 112 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/g 418 antibiotic selection/product/Thermo Fisher
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    g 418 antibiotic selection - by Bioz Stars, 2020-07
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    99
    Millipore geneticin selective antibiotic
    Sperm of Ppatg5 lines are infertile whereas the Ppatg5 egg upon fertilization, can develop into a functional sporophyte that produces viable spores. (A) differential interference contrast micrographs of representative gametophore apices 40 d after induction of reproductive development. Self-fertilization of Ppatg5 lines always fails. The red mark highlights the difference in size between a nonfertilized archegonium and a young sporophyte from a successful fertilization. Sporophyte maturation after successful female Ppatg5 x male WT crosses progresses much slower than after self-fertilization of WT. Scale bar: 200 µm (B) Sporophyte development comparison when WT is fully mature and has already burst. Scale bar: 1 mm. (C) Germinated spores in the presence or absence of selection marker <t>(geneticin),</t> which selects for genotypes carrying the Ppatg5 KO construct. (D) RT-PCR confirmation of colony genotype using primers p5 and p6. For principal positions of annealing sites and sequence of primers, see Figure S2A and Table S1.
    Geneticin Selective Antibiotic, supplied by Millipore, used in various techniques. Bioz Stars score: 99/100, based on 5 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/geneticin selective antibiotic/product/Millipore
    Average 99 stars, based on 5 article reviews
    Price from $9.99 to $1999.99
    geneticin selective antibiotic - by Bioz Stars, 2020-07
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    93
    Merck & Co geneticin selective antibiotic
    Sperm of Ppatg5 lines are infertile whereas the Ppatg5 egg upon fertilization, can develop into a functional sporophyte that produces viable spores. (A) differential interference contrast micrographs of representative gametophore apices 40 d after induction of reproductive development. Self-fertilization of Ppatg5 lines always fails. The red mark highlights the difference in size between a nonfertilized archegonium and a young sporophyte from a successful fertilization. Sporophyte maturation after successful female Ppatg5 x male WT crosses progresses much slower than after self-fertilization of WT. Scale bar: 200 µm (B) Sporophyte development comparison when WT is fully mature and has already burst. Scale bar: 1 mm. (C) Germinated spores in the presence or absence of selection marker <t>(geneticin),</t> which selects for genotypes carrying the Ppatg5 KO construct. (D) RT-PCR confirmation of colony genotype using primers p5 and p6. For principal positions of annealing sites and sequence of primers, see Figure S2A and Table S1.
    Geneticin Selective Antibiotic, supplied by Merck & Co, used in various techniques. Bioz Stars score: 93/100, based on 8 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/geneticin selective antibiotic/product/Merck & Co
    Average 93 stars, based on 8 article reviews
    Price from $9.99 to $1999.99
    geneticin selective antibiotic - by Bioz Stars, 2020-07
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    99
    Thermo Fisher geneticin antibiotic
    Sperm of Ppatg5 lines are infertile whereas the Ppatg5 egg upon fertilization, can develop into a functional sporophyte that produces viable spores. (A) differential interference contrast micrographs of representative gametophore apices 40 d after induction of reproductive development. Self-fertilization of Ppatg5 lines always fails. The red mark highlights the difference in size between a nonfertilized archegonium and a young sporophyte from a successful fertilization. Sporophyte maturation after successful female Ppatg5 x male WT crosses progresses much slower than after self-fertilization of WT. Scale bar: 200 µm (B) Sporophyte development comparison when WT is fully mature and has already burst. Scale bar: 1 mm. (C) Germinated spores in the presence or absence of selection marker <t>(geneticin),</t> which selects for genotypes carrying the Ppatg5 KO construct. (D) RT-PCR confirmation of colony genotype using primers p5 and p6. For principal positions of annealing sites and sequence of primers, see Figure S2A and Table S1.
    Geneticin Antibiotic, supplied by Thermo Fisher, used in various techniques. Bioz Stars score: 99/100, based on 39 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/geneticin antibiotic/product/Thermo Fisher
    Average 99 stars, based on 39 article reviews
    Price from $9.99 to $1999.99
    geneticin antibiotic - by Bioz Stars, 2020-07
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    94
    Promega selection antibiotic g 418
    Sperm of Ppatg5 lines are infertile whereas the Ppatg5 egg upon fertilization, can develop into a functional sporophyte that produces viable spores. (A) differential interference contrast micrographs of representative gametophore apices 40 d after induction of reproductive development. Self-fertilization of Ppatg5 lines always fails. The red mark highlights the difference in size between a nonfertilized archegonium and a young sporophyte from a successful fertilization. Sporophyte maturation after successful female Ppatg5 x male WT crosses progresses much slower than after self-fertilization of WT. Scale bar: 200 µm (B) Sporophyte development comparison when WT is fully mature and has already burst. Scale bar: 1 mm. (C) Germinated spores in the presence or absence of selection marker <t>(geneticin),</t> which selects for genotypes carrying the Ppatg5 KO construct. (D) RT-PCR confirmation of colony genotype using primers p5 and p6. For principal positions of annealing sites and sequence of primers, see Figure S2A and Table S1.
    Selection Antibiotic G 418, supplied by Promega, used in various techniques. Bioz Stars score: 94/100, based on 7 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    95
    Thermo Fisher geneticin
    Establishment of murine-derived primary epithelial cells with constitutive expression of HPV58E7. (A) Schematic diagram showing the workflow of establishment of BRK-HPV58E7 cells. Kidneys of 9-day-old Wistar Hannover rats were extracted, excised, trypsinized, and plated onto tissue culture dishes. After overnight culture, the attached monolayer cells were transfected with plasmids expressing EJ-Ras and HPV58E7 (prototype or V1, V2, or V3 variant). The cells were cultured under <t>Geneticin</t> (G418) selection. Upon reaching 90% confluence, G418-resistant cells were transferred and cultured in tissue culture flasks. The cells were subcultured every 3 to 4 days under continuous G418 selection. During the culturing process, clones with keratinocyte-like morphology and HPV58E7 expression were maintained for more than 30 passages ( > P30). These cell lines were used for cell proliferation, migration and invasion, tumor formation and molecular mechanism analyses. (B) Morphology of the BRK-HPV58E7 prototype (58E7P) and its variants BRK-HPV58E7 T20I/G63S (58E7V1), BRK-HPV58E7 G41R/G63D (58E7V2), and BRK-HPV58E7 T74A/D76E (58E7V3) observed under ×100 magnification. Note that the cells display keratinocyte-like morphology and polygonal shape and grow in monolayers. A cell line transfected with pcDNA3.1 empty vector plus EJ-Ras expressed exogenously (BRK-pcDNA3.1) was included as a negative control. (C) Total cell lysates were collected and subjected to Western blotting with pan-keratin (cytokeratin marker), S100A4 (fibroblast marker), and HPV58E7-specific antibodies.
    Geneticin, supplied by Thermo Fisher, used in various techniques. Bioz Stars score: 95/100, based on 27838 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/geneticin/product/Thermo Fisher
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    90
    Millipore geneticin antibiotic
    Establishment of murine-derived primary epithelial cells with constitutive expression of HPV58E7. (A) Schematic diagram showing the workflow of establishment of BRK-HPV58E7 cells. Kidneys of 9-day-old Wistar Hannover rats were extracted, excised, trypsinized, and plated onto tissue culture dishes. After overnight culture, the attached monolayer cells were transfected with plasmids expressing EJ-Ras and HPV58E7 (prototype or V1, V2, or V3 variant). The cells were cultured under <t>Geneticin</t> (G418) selection. Upon reaching 90% confluence, G418-resistant cells were transferred and cultured in tissue culture flasks. The cells were subcultured every 3 to 4 days under continuous G418 selection. During the culturing process, clones with keratinocyte-like morphology and HPV58E7 expression were maintained for more than 30 passages ( > P30). These cell lines were used for cell proliferation, migration and invasion, tumor formation and molecular mechanism analyses. (B) Morphology of the BRK-HPV58E7 prototype (58E7P) and its variants BRK-HPV58E7 T20I/G63S (58E7V1), BRK-HPV58E7 G41R/G63D (58E7V2), and BRK-HPV58E7 T74A/D76E (58E7V3) observed under ×100 magnification. Note that the cells display keratinocyte-like morphology and polygonal shape and grow in monolayers. A cell line transfected with pcDNA3.1 empty vector plus EJ-Ras expressed exogenously (BRK-pcDNA3.1) was included as a negative control. (C) Total cell lysates were collected and subjected to Western blotting with pan-keratin (cytokeratin marker), S100A4 (fibroblast marker), and HPV58E7-specific antibodies.
    Geneticin Antibiotic, supplied by Millipore, used in various techniques. Bioz Stars score: 90/100, based on 28 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/geneticin antibiotic/product/Millipore
    Average 90 stars, based on 28 article reviews
    Price from $9.99 to $1999.99
    geneticin antibiotic - by Bioz Stars, 2020-07
    90/100 stars
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    Image Search Results


    Sperm of Ppatg5 lines are infertile whereas the Ppatg5 egg upon fertilization, can develop into a functional sporophyte that produces viable spores. (A) differential interference contrast micrographs of representative gametophore apices 40 d after induction of reproductive development. Self-fertilization of Ppatg5 lines always fails. The red mark highlights the difference in size between a nonfertilized archegonium and a young sporophyte from a successful fertilization. Sporophyte maturation after successful female Ppatg5 x male WT crosses progresses much slower than after self-fertilization of WT. Scale bar: 200 µm (B) Sporophyte development comparison when WT is fully mature and has already burst. Scale bar: 1 mm. (C) Germinated spores in the presence or absence of selection marker (geneticin), which selects for genotypes carrying the Ppatg5 KO construct. (D) RT-PCR confirmation of colony genotype using primers p5 and p6. For principal positions of annealing sites and sequence of primers, see Figure S2A and Table S1.

    Journal: Autophagy

    Article Title: Autophagy is required for gamete differentiation in the moss Physcomitrella patens

    doi: 10.1080/15548627.2017.1366406

    Figure Lengend Snippet: Sperm of Ppatg5 lines are infertile whereas the Ppatg5 egg upon fertilization, can develop into a functional sporophyte that produces viable spores. (A) differential interference contrast micrographs of representative gametophore apices 40 d after induction of reproductive development. Self-fertilization of Ppatg5 lines always fails. The red mark highlights the difference in size between a nonfertilized archegonium and a young sporophyte from a successful fertilization. Sporophyte maturation after successful female Ppatg5 x male WT crosses progresses much slower than after self-fertilization of WT. Scale bar: 200 µm (B) Sporophyte development comparison when WT is fully mature and has already burst. Scale bar: 1 mm. (C) Germinated spores in the presence or absence of selection marker (geneticin), which selects for genotypes carrying the Ppatg5 KO construct. (D) RT-PCR confirmation of colony genotype using primers p5 and p6. For principal positions of annealing sites and sequence of primers, see Figure S2A and Table S1.

    Article Snippet: Transformants were selected on MM plates supplemented with geneticin (Thermo Fisher Scientific, 11811023) at a concentration of 50 µg/ml.

    Techniques: Functional Assay, Selection, Marker, Construct, Reverse Transcription Polymerase Chain Reaction, Sequencing

    Establishment of murine-derived primary epithelial cells with constitutive expression of HPV58E7. (A) Schematic diagram showing the workflow of establishment of BRK-HPV58E7 cells. Kidneys of 9-day-old Wistar Hannover rats were extracted, excised, trypsinized, and plated onto tissue culture dishes. After overnight culture, the attached monolayer cells were transfected with plasmids expressing EJ-Ras and HPV58E7 (prototype or V1, V2, or V3 variant). The cells were cultured under Geneticin (G418) selection. Upon reaching 90% confluence, G418-resistant cells were transferred and cultured in tissue culture flasks. The cells were subcultured every 3 to 4 days under continuous G418 selection. During the culturing process, clones with keratinocyte-like morphology and HPV58E7 expression were maintained for more than 30 passages ( > P30). These cell lines were used for cell proliferation, migration and invasion, tumor formation and molecular mechanism analyses. (B) Morphology of the BRK-HPV58E7 prototype (58E7P) and its variants BRK-HPV58E7 T20I/G63S (58E7V1), BRK-HPV58E7 G41R/G63D (58E7V2), and BRK-HPV58E7 T74A/D76E (58E7V3) observed under ×100 magnification. Note that the cells display keratinocyte-like morphology and polygonal shape and grow in monolayers. A cell line transfected with pcDNA3.1 empty vector plus EJ-Ras expressed exogenously (BRK-pcDNA3.1) was included as a negative control. (C) Total cell lysates were collected and subjected to Western blotting with pan-keratin (cytokeratin marker), S100A4 (fibroblast marker), and HPV58E7-specific antibodies.

    Journal: Journal of Virology

    Article Title: Human Papillomavirus 58 E7 T20I/G63S Variant Isolated from an East Asian Population Possesses High Oncogenicity

    doi: 10.1128/JVI.00090-20

    Figure Lengend Snippet: Establishment of murine-derived primary epithelial cells with constitutive expression of HPV58E7. (A) Schematic diagram showing the workflow of establishment of BRK-HPV58E7 cells. Kidneys of 9-day-old Wistar Hannover rats were extracted, excised, trypsinized, and plated onto tissue culture dishes. After overnight culture, the attached monolayer cells were transfected with plasmids expressing EJ-Ras and HPV58E7 (prototype or V1, V2, or V3 variant). The cells were cultured under Geneticin (G418) selection. Upon reaching 90% confluence, G418-resistant cells were transferred and cultured in tissue culture flasks. The cells were subcultured every 3 to 4 days under continuous G418 selection. During the culturing process, clones with keratinocyte-like morphology and HPV58E7 expression were maintained for more than 30 passages ( > P30). These cell lines were used for cell proliferation, migration and invasion, tumor formation and molecular mechanism analyses. (B) Morphology of the BRK-HPV58E7 prototype (58E7P) and its variants BRK-HPV58E7 T20I/G63S (58E7V1), BRK-HPV58E7 G41R/G63D (58E7V2), and BRK-HPV58E7 T74A/D76E (58E7V3) observed under ×100 magnification. Note that the cells display keratinocyte-like morphology and polygonal shape and grow in monolayers. A cell line transfected with pcDNA3.1 empty vector plus EJ-Ras expressed exogenously (BRK-pcDNA3.1) was included as a negative control. (C) Total cell lysates were collected and subjected to Western blotting with pan-keratin (cytokeratin marker), S100A4 (fibroblast marker), and HPV58E7-specific antibodies.

    Article Snippet: The cells were also cultured under a continuous selection of 220 μg/ml Geneticin (G418; ThermoFisher Scientific).

    Techniques: Derivative Assay, Expressing, Transfection, Variant Assay, Cell Culture, Selection, Clone Assay, Migration, Plasmid Preparation, Negative Control, Western Blot, Marker