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  • 99
    Qiagen genereader actionable insights panel
    Mutation detection in HCT116 cancer cells with different DNA input using <t>GeneRead</t> <t>DNAseq</t> targeted <t>CRC</t> Panel. Forty and 1 ng DNA extracted from fresh HCT116 cells were compared to 1.0, 0.5, and 0.2 ng of DNA extracted from HCT116 cells fixed with 4% PFA. In all conditions, DNA was subjected to multiplex PCR, library preparation and MiSeq sequencing. Mutations and corresponding allele frequencies were analyzed and compared
    Genereader Actionable Insights Panel, supplied by Qiagen, used in various techniques. Bioz Stars score: 99/100, based on 17 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/genereader actionable insights panel/product/Qiagen
    Average 99 stars, based on 17 article reviews
    Price from $9.99 to $1999.99
    genereader actionable insights panel - by Bioz Stars, 2020-08
    99/100 stars
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    95
    Qiagen generead qiaact brca 1 2 panel
    Mutation detection in HCT116 cancer cells with different DNA input using <t>GeneRead</t> <t>DNAseq</t> targeted <t>CRC</t> Panel. Forty and 1 ng DNA extracted from fresh HCT116 cells were compared to 1.0, 0.5, and 0.2 ng of DNA extracted from HCT116 cells fixed with 4% PFA. In all conditions, DNA was subjected to multiplex PCR, library preparation and MiSeq sequencing. Mutations and corresponding allele frequencies were analyzed and compared
    Generead Qiaact Brca 1 2 Panel, supplied by Qiagen, used in various techniques. Bioz Stars score: 95/100, based on 3 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/generead qiaact brca 1 2 panel/product/Qiagen
    Average 95 stars, based on 3 article reviews
    Price from $9.99 to $1999.99
    generead qiaact brca 1 2 panel - by Bioz Stars, 2020-08
    95/100 stars
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    99
    Qiagen generead dnaseq panel pcr kit
    Scheme of primer design and the multiplex <t>PCR-based</t> approach to mt-genome enrichment. Primer sets (Table S2 ) were designed ( A ) generating 108 amplicons spanning the whole mitochondrial genome ( B ). Four primer pools, each including 27 primer sets, were applied to multiplex PCR according to the <t>GeneRead</t> multiplex PCR design of Qiagen, to ensure that overlapping amplicons were generated in separate reaction mixes.
    Generead Dnaseq Panel Pcr Kit, supplied by Qiagen, used in various techniques. Bioz Stars score: 99/100, based on 73 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/generead dnaseq panel pcr kit/product/Qiagen
    Average 99 stars, based on 73 article reviews
    Price from $9.99 to $1999.99
    generead dnaseq panel pcr kit - by Bioz Stars, 2020-08
    99/100 stars
      Buy from Supplier

    94
    Qiagen generead qiaact lung dna panel
    Scheme of primer design and the multiplex <t>PCR-based</t> approach to mt-genome enrichment. Primer sets (Table S2 ) were designed ( A ) generating 108 amplicons spanning the whole mitochondrial genome ( B ). Four primer pools, each including 27 primer sets, were applied to multiplex PCR according to the <t>GeneRead</t> multiplex PCR design of Qiagen, to ensure that overlapping amplicons were generated in separate reaction mixes.
    Generead Qiaact Lung Dna Panel, supplied by Qiagen, used in various techniques. Bioz Stars score: 94/100, based on 6 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/generead qiaact lung dna panel/product/Qiagen
    Average 94 stars, based on 6 article reviews
    Price from $9.99 to $1999.99
    generead qiaact lung dna panel - by Bioz Stars, 2020-08
    94/100 stars
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    99
    Qiagen generead gene panel mastermix
    Scheme of primer design and the multiplex <t>PCR-based</t> approach to mt-genome enrichment. Primer sets (Table S2 ) were designed ( A ) generating 108 amplicons spanning the whole mitochondrial genome ( B ). Four primer pools, each including 27 primer sets, were applied to multiplex PCR according to the <t>GeneRead</t> multiplex PCR design of Qiagen, to ensure that overlapping amplicons were generated in separate reaction mixes.
    Generead Gene Panel Mastermix, supplied by Qiagen, used in various techniques. Bioz Stars score: 99/100, based on 8 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/generead gene panel mastermix/product/Qiagen
    Average 99 stars, based on 8 article reviews
    Price from $9.99 to $1999.99
    generead gene panel mastermix - by Bioz Stars, 2020-08
    99/100 stars
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    91
    Qiagen generead qiaact actionable insight tumor panel
    Scheme of primer design and the multiplex <t>PCR-based</t> approach to mt-genome enrichment. Primer sets (Table S2 ) were designed ( A ) generating 108 amplicons spanning the whole mitochondrial genome ( B ). Four primer pools, each including 27 primer sets, were applied to multiplex PCR according to the <t>GeneRead</t> multiplex PCR design of Qiagen, to ensure that overlapping amplicons were generated in separate reaction mixes.
    Generead Qiaact Actionable Insight Tumor Panel, supplied by Qiagen, used in various techniques. Bioz Stars score: 91/100, based on 4 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/generead qiaact actionable insight tumor panel/product/Qiagen
    Average 91 stars, based on 4 article reviews
    Price from $9.99 to $1999.99
    generead qiaact actionable insight tumor panel - by Bioz Stars, 2020-08
    91/100 stars
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    Image Search Results


    Mutation detection in HCT116 cancer cells with different DNA input using GeneRead DNAseq targeted CRC Panel. Forty and 1 ng DNA extracted from fresh HCT116 cells were compared to 1.0, 0.5, and 0.2 ng of DNA extracted from HCT116 cells fixed with 4% PFA. In all conditions, DNA was subjected to multiplex PCR, library preparation and MiSeq sequencing. Mutations and corresponding allele frequencies were analyzed and compared

    Journal: NPJ Genomic Medicine

    Article Title: Workflow optimization of whole genome amplification and targeted panel sequencing for CTC mutation detection

    doi: 10.1038/s41525-017-0034-3

    Figure Lengend Snippet: Mutation detection in HCT116 cancer cells with different DNA input using GeneRead DNAseq targeted CRC Panel. Forty and 1 ng DNA extracted from fresh HCT116 cells were compared to 1.0, 0.5, and 0.2 ng of DNA extracted from HCT116 cells fixed with 4% PFA. In all conditions, DNA was subjected to multiplex PCR, library preparation and MiSeq sequencing. Mutations and corresponding allele frequencies were analyzed and compared

    Article Snippet: When DNA amount is higher than 40 ng The whole workflow, including Qiagen GeneRead DNAseq CRC Targeted Panel v2 (NGHS-002X, Qiagen) and MiSeq NGS, was tested on HCT116 DNA, human genomic DNA and tumor tissue DNA with a DNA input of 40 ng as recommended (Fig. , Supplementary Figure , Fig. ).

    Techniques: Mutagenesis, Multiplex Assay, Polymerase Chain Reaction, Sequencing

    Mutation detection using GeneRead DNAseq targeted CRC panel sequencing. a Mutation detection in HCT116 cancer cells. Forty nanogram of DNA extracted from fresh HCT116 cells were subjected to multiplex PCR, library preparation and MiSeq sequencing. Mutations and corresponding allele frequencies were called using Ingenuity variant analysis software and compared to results reported from Cosmic* (catalog of somatic mutations in cancer) and ATCC websites. b Mutation detection in CRC patient biopsy tissues. Forty nanogram of DNA extracted from primary tumor and liver metastasis were subjected to multiplex PCR, library preparation and MiSeq sequencing. Mutations and corresponding Mutation Allele Fraction (MAF) were analyzed and compared between primary tumor and liver metastasis. In both tables, the blue color highlights the detected mutation and the number inside each cell represents the MAF of the mutation

    Journal: NPJ Genomic Medicine

    Article Title: Workflow optimization of whole genome amplification and targeted panel sequencing for CTC mutation detection

    doi: 10.1038/s41525-017-0034-3

    Figure Lengend Snippet: Mutation detection using GeneRead DNAseq targeted CRC panel sequencing. a Mutation detection in HCT116 cancer cells. Forty nanogram of DNA extracted from fresh HCT116 cells were subjected to multiplex PCR, library preparation and MiSeq sequencing. Mutations and corresponding allele frequencies were called using Ingenuity variant analysis software and compared to results reported from Cosmic* (catalog of somatic mutations in cancer) and ATCC websites. b Mutation detection in CRC patient biopsy tissues. Forty nanogram of DNA extracted from primary tumor and liver metastasis were subjected to multiplex PCR, library preparation and MiSeq sequencing. Mutations and corresponding Mutation Allele Fraction (MAF) were analyzed and compared between primary tumor and liver metastasis. In both tables, the blue color highlights the detected mutation and the number inside each cell represents the MAF of the mutation

    Article Snippet: When DNA amount is higher than 40 ng The whole workflow, including Qiagen GeneRead DNAseq CRC Targeted Panel v2 (NGHS-002X, Qiagen) and MiSeq NGS, was tested on HCT116 DNA, human genomic DNA and tumor tissue DNA with a DNA input of 40 ng as recommended (Fig. , Supplementary Figure , Fig. ).

    Techniques: Mutagenesis, Sequencing, Multiplex Assay, Polymerase Chain Reaction, Variant Assay, Software

    Scheme of primer design and the multiplex PCR-based approach to mt-genome enrichment. Primer sets (Table S2 ) were designed ( A ) generating 108 amplicons spanning the whole mitochondrial genome ( B ). Four primer pools, each including 27 primer sets, were applied to multiplex PCR according to the GeneRead multiplex PCR design of Qiagen, to ensure that overlapping amplicons were generated in separate reaction mixes.

    Journal: Scientific Reports

    Article Title: Evolution analysis of heterogeneous non-small cell lung carcinoma by ultra-deep sequencing of the mitochondrial genome

    doi: 10.1038/s41598-017-11345-3

    Figure Lengend Snippet: Scheme of primer design and the multiplex PCR-based approach to mt-genome enrichment. Primer sets (Table S2 ) were designed ( A ) generating 108 amplicons spanning the whole mitochondrial genome ( B ). Four primer pools, each including 27 primer sets, were applied to multiplex PCR according to the GeneRead multiplex PCR design of Qiagen, to ensure that overlapping amplicons were generated in separate reaction mixes.

    Article Snippet: For enrichment of the mt-genome by a multiplex PCR, primer sets were pooled in four primer mixes of 2 µM and in each reaction, 10 ng of PCR accessible DNA -representing DNA of around 1500 cells was used (Fig. ). mtDNA was then amplified in four separate multiplex PCR reactions per sample using the GeneRead DNAseq Panel PCR Kit (QIAGEN Inc., Hilden, GER) in accordance with the manufacturer´s protocol.

    Techniques: Multiplex Assay, Polymerase Chain Reaction, Generated