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  • 99
    Thermo Fisher gene jet pcr purification kit
    Gene Jet Pcr Purification Kit, supplied by Thermo Fisher, used in various techniques. Bioz Stars score: 99/100, based on 973 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    Thermo Fisher gene jet gel extraction kit
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    Thermo Fisher gene jet rna purification kit
    Absence of neuroinflammation in RNase H2 Δ GFAP mice. (A) Immunohistochemistry for the astrocyte marker GFAP reveals absence of significant astrogliosis in the hippocampus, cerebral cortex, cerebellum, and thalamus of RNase H2 ΔGFAP mice. Astrogliosis would present as increased expression of GFAP, cellular hypertrophy with changes of astrocyte morphology or proliferation of astrocytes. (B) Comparable Iba-1 staining between RNase H2 ΔGFAP and control brains indicates absence of microgliosis in RNase H2 ΔGFAP mice (age 3 months). Inserts represent magnifications of individual hippocampal microglia showing similar morphology in both genotypes. Scale bar = 150 µm. (C) Comparable mRNA expression of IFIT1, IRF7, and CXCL10 in RNase H2 ΔGFAP and control brains ( x = 1), as quantified by <t>qPCR.</t> ISG expression was normalized to GAPDH. Whole brain <t>RNA</t> was prepared from P3-4 pups. Error bars are SEM, t -test ( n = 5 brains/genotype).
    Gene Jet Rna Purification Kit, supplied by Thermo Fisher, used in various techniques. Bioz Stars score: 99/100, based on 991 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/gene jet rna purification kit/product/Thermo Fisher
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    Thermo Fisher gene jet genomic dna purification kit
    Absence of neuroinflammation in RNase H2 Δ GFAP mice. (A) Immunohistochemistry for the astrocyte marker GFAP reveals absence of significant astrogliosis in the hippocampus, cerebral cortex, cerebellum, and thalamus of RNase H2 ΔGFAP mice. Astrogliosis would present as increased expression of GFAP, cellular hypertrophy with changes of astrocyte morphology or proliferation of astrocytes. (B) Comparable Iba-1 staining between RNase H2 ΔGFAP and control brains indicates absence of microgliosis in RNase H2 ΔGFAP mice (age 3 months). Inserts represent magnifications of individual hippocampal microglia showing similar morphology in both genotypes. Scale bar = 150 µm. (C) Comparable mRNA expression of IFIT1, IRF7, and CXCL10 in RNase H2 ΔGFAP and control brains ( x = 1), as quantified by <t>qPCR.</t> ISG expression was normalized to GAPDH. Whole brain <t>RNA</t> was prepared from P3-4 pups. Error bars are SEM, t -test ( n = 5 brains/genotype).
    Gene Jet Genomic Dna Purification Kit, supplied by Thermo Fisher, used in various techniques. Bioz Stars score: 99/100, based on 415 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    Thermo Fisher gene jet plasmid miniprep kit
    Absence of neuroinflammation in RNase H2 Δ GFAP mice. (A) Immunohistochemistry for the astrocyte marker GFAP reveals absence of significant astrogliosis in the hippocampus, cerebral cortex, cerebellum, and thalamus of RNase H2 ΔGFAP mice. Astrogliosis would present as increased expression of GFAP, cellular hypertrophy with changes of astrocyte morphology or proliferation of astrocytes. (B) Comparable Iba-1 staining between RNase H2 ΔGFAP and control brains indicates absence of microgliosis in RNase H2 ΔGFAP mice (age 3 months). Inserts represent magnifications of individual hippocampal microglia showing similar morphology in both genotypes. Scale bar = 150 µm. (C) Comparable mRNA expression of IFIT1, IRF7, and CXCL10 in RNase H2 ΔGFAP and control brains ( x = 1), as quantified by <t>qPCR.</t> ISG expression was normalized to GAPDH. Whole brain <t>RNA</t> was prepared from P3-4 pups. Error bars are SEM, t -test ( n = 5 brains/genotype).
    Gene Jet Plasmid Miniprep Kit, supplied by Thermo Fisher, used in various techniques. Bioz Stars score: 99/100, based on 1536 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    Thermo Fisher gene jet extraction kit
    Absence of neuroinflammation in RNase H2 Δ GFAP mice. (A) Immunohistochemistry for the astrocyte marker GFAP reveals absence of significant astrogliosis in the hippocampus, cerebral cortex, cerebellum, and thalamus of RNase H2 ΔGFAP mice. Astrogliosis would present as increased expression of GFAP, cellular hypertrophy with changes of astrocyte morphology or proliferation of astrocytes. (B) Comparable Iba-1 staining between RNase H2 ΔGFAP and control brains indicates absence of microgliosis in RNase H2 ΔGFAP mice (age 3 months). Inserts represent magnifications of individual hippocampal microglia showing similar morphology in both genotypes. Scale bar = 150 µm. (C) Comparable mRNA expression of IFIT1, IRF7, and CXCL10 in RNase H2 ΔGFAP and control brains ( x = 1), as quantified by <t>qPCR.</t> ISG expression was normalized to GAPDH. Whole brain <t>RNA</t> was prepared from P3-4 pups. Error bars are SEM, t -test ( n = 5 brains/genotype).
    Gene Jet Extraction Kit, supplied by Thermo Fisher, used in various techniques. Bioz Stars score: 99/100, based on 766 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    Thermo Fisher invitrap spin plant mini kit
    Absence of neuroinflammation in RNase H2 Δ GFAP mice. (A) Immunohistochemistry for the astrocyte marker GFAP reveals absence of significant astrogliosis in the hippocampus, cerebral cortex, cerebellum, and thalamus of RNase H2 ΔGFAP mice. Astrogliosis would present as increased expression of GFAP, cellular hypertrophy with changes of astrocyte morphology or proliferation of astrocytes. (B) Comparable Iba-1 staining between RNase H2 ΔGFAP and control brains indicates absence of microgliosis in RNase H2 ΔGFAP mice (age 3 months). Inserts represent magnifications of individual hippocampal microglia showing similar morphology in both genotypes. Scale bar = 150 µm. (C) Comparable mRNA expression of IFIT1, IRF7, and CXCL10 in RNase H2 ΔGFAP and control brains ( x = 1), as quantified by <t>qPCR.</t> ISG expression was normalized to GAPDH. Whole brain <t>RNA</t> was prepared from P3-4 pups. Error bars are SEM, t -test ( n = 5 brains/genotype).
    Invitrap Spin Plant Mini Kit, supplied by Thermo Fisher, used in various techniques. Bioz Stars score: 91/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    Thermo Fisher using gene jet whole blood genomic dna purification mini kit
    Absence of neuroinflammation in RNase H2 Δ GFAP mice. (A) Immunohistochemistry for the astrocyte marker GFAP reveals absence of significant astrogliosis in the hippocampus, cerebral cortex, cerebellum, and thalamus of RNase H2 ΔGFAP mice. Astrogliosis would present as increased expression of GFAP, cellular hypertrophy with changes of astrocyte morphology or proliferation of astrocytes. (B) Comparable Iba-1 staining between RNase H2 ΔGFAP and control brains indicates absence of microgliosis in RNase H2 ΔGFAP mice (age 3 months). Inserts represent magnifications of individual hippocampal microglia showing similar morphology in both genotypes. Scale bar = 150 µm. (C) Comparable mRNA expression of IFIT1, IRF7, and CXCL10 in RNase H2 ΔGFAP and control brains ( x = 1), as quantified by <t>qPCR.</t> ISG expression was normalized to GAPDH. Whole brain <t>RNA</t> was prepared from P3-4 pups. Error bars are SEM, t -test ( n = 5 brains/genotype).
    Using Gene Jet Whole Blood Genomic Dna Purification Mini Kit, supplied by Thermo Fisher, used in various techniques. Bioz Stars score: 99/100, based on 4 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    Image Search Results


    Absence of neuroinflammation in RNase H2 Δ GFAP mice. (A) Immunohistochemistry for the astrocyte marker GFAP reveals absence of significant astrogliosis in the hippocampus, cerebral cortex, cerebellum, and thalamus of RNase H2 ΔGFAP mice. Astrogliosis would present as increased expression of GFAP, cellular hypertrophy with changes of astrocyte morphology or proliferation of astrocytes. (B) Comparable Iba-1 staining between RNase H2 ΔGFAP and control brains indicates absence of microgliosis in RNase H2 ΔGFAP mice (age 3 months). Inserts represent magnifications of individual hippocampal microglia showing similar morphology in both genotypes. Scale bar = 150 µm. (C) Comparable mRNA expression of IFIT1, IRF7, and CXCL10 in RNase H2 ΔGFAP and control brains ( x = 1), as quantified by qPCR. ISG expression was normalized to GAPDH. Whole brain RNA was prepared from P3-4 pups. Error bars are SEM, t -test ( n = 5 brains/genotype).

    Journal: Frontiers in Immunology

    Article Title: RNase H2 Loss in Murine Astrocytes Results in Cellular Defects Reminiscent of Nucleic Acid-Mediated Autoinflammation

    doi: 10.3389/fimmu.2018.00587

    Figure Lengend Snippet: Absence of neuroinflammation in RNase H2 Δ GFAP mice. (A) Immunohistochemistry for the astrocyte marker GFAP reveals absence of significant astrogliosis in the hippocampus, cerebral cortex, cerebellum, and thalamus of RNase H2 ΔGFAP mice. Astrogliosis would present as increased expression of GFAP, cellular hypertrophy with changes of astrocyte morphology or proliferation of astrocytes. (B) Comparable Iba-1 staining between RNase H2 ΔGFAP and control brains indicates absence of microgliosis in RNase H2 ΔGFAP mice (age 3 months). Inserts represent magnifications of individual hippocampal microglia showing similar morphology in both genotypes. Scale bar = 150 µm. (C) Comparable mRNA expression of IFIT1, IRF7, and CXCL10 in RNase H2 ΔGFAP and control brains ( x = 1), as quantified by qPCR. ISG expression was normalized to GAPDH. Whole brain RNA was prepared from P3-4 pups. Error bars are SEM, t -test ( n = 5 brains/genotype).

    Article Snippet: qPCR, Western Blotting, and FACS Total RNA was isolated using the Gene Jet RNA Purification Kit (Thermo Fisher Scientific), while cDNA was generated with Revert Aid Reverse Transcriptase (Thermo Fisher Scientific) according to the manufacturer’s instructions, except a mixture of oligo(dT)18 and random hexamer primers (1:2, Thermo Fisher Scientific) was used.

    Techniques: Mouse Assay, Immunohistochemistry, Marker, Expressing, Staining, Real-time Polymerase Chain Reaction