Journal: Nutrition & Diabetes
Article Title: Human mediastinal adipose tissue displays certain characteristics of brown fat
Figure Lengend Snippet: Comparison of gene expression in human subcutaneous and mediastinal adipose tissue. Real-time PCR validation of genes selected from the microarray analysis. Each dot represents one individual ( n =23). Box plots represent median (thick black lines), first and third quartiles (outlined boxes), the lowest data point still within 1.5 times the interquartile range from the first quartile (lower whiskers) and the highest data point still within 1.5 times the interquartile range from the third quartile (upper whiskers) of the expression levels of UCP1 , PPARGC1A , CIDEA , PRDM16 , S HOX2 and HOXC8 in subcutaneous and mediastinal adipose tissue. Gene expression was normalized to reference gene PPIA . P -values were calculated according to Wilcoxon paired-sample test.
Article Snippet: Quantitative PCR RNA samples from a separate group of 23 patients (see description in ) were used for complementary DNA synthesis with SuperScript III (Invitrogen) and analysed with TaqMan gene expression assays (PPIA : Hs99999904_m1, TBP : Hs00427620_m1, UCP1 : Hs00222453_m1, PRDM16 : Hs00223161_m1, COBL: Hs00391205_m1, CIDEA : Hs00154455_m1, PPARGC1A : Hs00222453_m1, SHOX2 : Hs00243203_m1 and HOXC8 : Hs00224073_m1; Applied Biosystems, Foster City, CA, USA).
Techniques: Expressing, Real-time Polymerase Chain Reaction, Microarray