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    Thermo Fisher gene exp gapdh hs02758991 g1
    A1M expression is induced in human aortic smooth muscle cells (HAoSMCs). HAoSMCs were exposed to heme (10 µM), OxyHb (10 µM), or FerrylHb (10 µM) in the presence of 5% foetal calf serum. A1M ( A ) and HO-1 ( B ) mRNA expression was quantified by qPCR after 4, 8, 24 and 48 h. ( C ) Secreted A1M protein was detected by immunoblotting in cell culture supernatants, while HO-1 protein expression ( D ) was examined in cell lysates after 24 and 48 h. GM: growth medium, BSA: bovine serum albumin, <t>GAPDH:</t> glyceraldehyde 3-phosphate dehydrogenase.
    Gene Exp Gapdh Hs02758991 G1, supplied by Thermo Fisher, used in various techniques. Bioz Stars score: 99/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/gene exp gapdh hs02758991 g1/product/Thermo Fisher
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    99
    Bio-Rad gene exp gapdh hs02758991 g1
    A1M expression is induced in human aortic smooth muscle cells (HAoSMCs). HAoSMCs were exposed to heme (10 µM), OxyHb (10 µM), or FerrylHb (10 µM) in the presence of 5% foetal calf serum. A1M ( A ) and HO-1 ( B ) mRNA expression was quantified by qPCR after 4, 8, 24 and 48 h. ( C ) Secreted A1M protein was detected by immunoblotting in cell culture supernatants, while HO-1 protein expression ( D ) was examined in cell lysates after 24 and 48 h. GM: growth medium, BSA: bovine serum albumin, <t>GAPDH:</t> glyceraldehyde 3-phosphate dehydrogenase.
    Gene Exp Gapdh Hs02758991 G1, supplied by Bio-Rad, used in various techniques. Bioz Stars score: 99/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/gene exp gapdh hs02758991 g1/product/Bio-Rad
    Average 99 stars, based on 1 article reviews
    Price from $9.99 to $1999.99
    gene exp gapdh hs02758991 g1 - by Bioz Stars, 2021-07
    99/100 stars
      Buy from Supplier

    86
    Roche gene exp gapdh hs02758991 g1
    ABT-751 inhibits SKP2 transcription and subsequent translation in UBUC cells through dysregulation of the AKT–CHUK–NFKBIA–NFKB signaling pathway. ( A ) BFTC905 cells were treated with ABT-751 (0.6 μM) for 24 h and the levels of the components of SKP2 E3 ligase (CDC20 and FZR1 proteins) were determined by immunoblots. ( B ) The cells were pretreated with 4 μM of MG132, a proteasome inhibitor, for 6 h, then treated with a specified concentration of ABT-751 (BFTC905, 0.6 μM and J82, 0.7 μM) for another 18 h. Next, nuclear/cytosol fractionation along with immunoblot analysis were performed. ( C ) The mRNA levels of SKP2 , TP 53, CDKN 1 A , CDKN 1 B , RB 1, E 2 F 1 and TFDP 1 were analyzed by quantitative RT-PCR. ( D ) The expression levels of the SKP2 gene regulator-associated proteins were determined by immunoblots in BFTC905 and J82 cells. Cells were treated with a specified concentration of ABT-751 (BFTC905, 0.6 μM and J82, 0.7 μM) for 24 h, ( E ) AKT1 kinase activity via a co-immunoprecipitation assay and ( F ) nuclear/cytosol fractionation along with immunoblot analysis were performed. ( G ) The cells were treated with a specified concentration of ABT-751 (BFTC905, 0.6 μM and J82, 0.7 μM) for 24 h, cell lysates were subjected to the co-immunoprecipitation assay with anti-RELA antibody. ( H ) Immunoblot analysis was used to compare the alterations of SKP2 -regulatory protein levels after treatment with a PI3K-AKT inhibitor, LY294002 (10 μM) for 2 h and ABT-751 (BFTC905, 0.6 μM and J82, 0.7 μM) for 24 h. ( I ) BFTC905 cells were treated with ABT-751 (0.6 μM) or LY294002 (10 μM) and SKP2 mRNA levels were analyzed by quantitative RT-PCR. ( J ) Transfection of a constitutive active AKT1 plasmid, pHRIG-AKT1, into BFTC905 cells for 24 h, suppressed AKT1-induced SKP2 mRNA levels. Relative protein levels were quantified using Image J software. The results are expressed as the means ± SD. Pan-actin, <t>GAPDH</t> and PARP1 served as loading, cytosolic and nuclear controls, respectively, in each gel. Statistical significance: * p
    Gene Exp Gapdh Hs02758991 G1, supplied by Roche, used in various techniques. Bioz Stars score: 86/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    Average 86 stars, based on 1 article reviews
    Price from $9.99 to $1999.99
    gene exp gapdh hs02758991 g1 - by Bioz Stars, 2021-07
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    Image Search Results


    A1M expression is induced in human aortic smooth muscle cells (HAoSMCs). HAoSMCs were exposed to heme (10 µM), OxyHb (10 µM), or FerrylHb (10 µM) in the presence of 5% foetal calf serum. A1M ( A ) and HO-1 ( B ) mRNA expression was quantified by qPCR after 4, 8, 24 and 48 h. ( C ) Secreted A1M protein was detected by immunoblotting in cell culture supernatants, while HO-1 protein expression ( D ) was examined in cell lysates after 24 and 48 h. GM: growth medium, BSA: bovine serum albumin, GAPDH: glyceraldehyde 3-phosphate dehydrogenase.

    Journal: International Journal of Molecular Sciences

    Article Title: Ferryl Hemoglobin and Heme Induce A1-Microglobulin in Hemorrhaged Atherosclerotic Lesions with Inhibitory Function against Hemoglobin and Lipid Oxidation

    doi: 10.3390/ijms22136668

    Figure Lengend Snippet: A1M expression is induced in human aortic smooth muscle cells (HAoSMCs). HAoSMCs were exposed to heme (10 µM), OxyHb (10 µM), or FerrylHb (10 µM) in the presence of 5% foetal calf serum. A1M ( A ) and HO-1 ( B ) mRNA expression was quantified by qPCR after 4, 8, 24 and 48 h. ( C ) Secreted A1M protein was detected by immunoblotting in cell culture supernatants, while HO-1 protein expression ( D ) was examined in cell lysates after 24 and 48 h. GM: growth medium, BSA: bovine serum albumin, GAPDH: glyceraldehyde 3-phosphate dehydrogenase.

    Article Snippet: Assay IDs for predesigned TaqMan Gene Expression Assays are as follows: AMBP: Hs00155697_m1; HO-1: Hs01110250; GAPDH: Hs02758991_g1.

    Techniques: Expressing, Real-time Polymerase Chain Reaction, Cell Culture

    A1M expression is induced in human aortic endothelial cells (HAoECs). HAoECs were exposed to heme (10 µM), OxyHb (10 µM), or FerrylHb (10 µM) in the presence of 5% foetal calf serum. A1M ( A ) and HO-1 ( B ) mRNA expression was quantified by qPCR after 4, 8, 24 and 48 h. ( C ) Secreted A1M protein was detected by immunoblotting in cell culture supernatants, while HO-1 protein level ( D ) was examined in cell lysates after 24 and 48 h. GM: growth medium, BSA: bovine serum albumin, GAPDH: glyceraldehyde 3-phosphate dehydrogenase.

    Journal: International Journal of Molecular Sciences

    Article Title: Ferryl Hemoglobin and Heme Induce A1-Microglobulin in Hemorrhaged Atherosclerotic Lesions with Inhibitory Function against Hemoglobin and Lipid Oxidation

    doi: 10.3390/ijms22136668

    Figure Lengend Snippet: A1M expression is induced in human aortic endothelial cells (HAoECs). HAoECs were exposed to heme (10 µM), OxyHb (10 µM), or FerrylHb (10 µM) in the presence of 5% foetal calf serum. A1M ( A ) and HO-1 ( B ) mRNA expression was quantified by qPCR after 4, 8, 24 and 48 h. ( C ) Secreted A1M protein was detected by immunoblotting in cell culture supernatants, while HO-1 protein level ( D ) was examined in cell lysates after 24 and 48 h. GM: growth medium, BSA: bovine serum albumin, GAPDH: glyceraldehyde 3-phosphate dehydrogenase.

    Article Snippet: Assay IDs for predesigned TaqMan Gene Expression Assays are as follows: AMBP: Hs00155697_m1; HO-1: Hs01110250; GAPDH: Hs02758991_g1.

    Techniques: Expressing, Real-time Polymerase Chain Reaction, Cell Culture

    A1M expression is induced in human peripheral blood mononuclear cells (PBMCs). PBMCs were exposed to heme (10 µM), OxyHb (10 µM), or FerrylHb (10 µM) in the presence of 5% foetal calf serum. A1M ( A ) and HO-1 ( B ) mRNA expression was quantified by qPCR after 4, 8, 24 and 48 h. ( C ) Secreted A1M protein was detected by immunoblotting in cell culture supernatants, while HO-1 protein expression ( D ) was examined in cell lysates after 24 and 48 h. GM: growth medium, BSA: bovine serum albumin, GAPDH: glyceraldehyde 3-phosphate dehydrogenase.

    Journal: International Journal of Molecular Sciences

    Article Title: Ferryl Hemoglobin and Heme Induce A1-Microglobulin in Hemorrhaged Atherosclerotic Lesions with Inhibitory Function against Hemoglobin and Lipid Oxidation

    doi: 10.3390/ijms22136668

    Figure Lengend Snippet: A1M expression is induced in human peripheral blood mononuclear cells (PBMCs). PBMCs were exposed to heme (10 µM), OxyHb (10 µM), or FerrylHb (10 µM) in the presence of 5% foetal calf serum. A1M ( A ) and HO-1 ( B ) mRNA expression was quantified by qPCR after 4, 8, 24 and 48 h. ( C ) Secreted A1M protein was detected by immunoblotting in cell culture supernatants, while HO-1 protein expression ( D ) was examined in cell lysates after 24 and 48 h. GM: growth medium, BSA: bovine serum albumin, GAPDH: glyceraldehyde 3-phosphate dehydrogenase.

    Article Snippet: Assay IDs for predesigned TaqMan Gene Expression Assays are as follows: AMBP: Hs00155697_m1; HO-1: Hs01110250; GAPDH: Hs02758991_g1.

    Techniques: Expressing, Real-time Polymerase Chain Reaction, Cell Culture

    ABT-751 inhibits SKP2 transcription and subsequent translation in UBUC cells through dysregulation of the AKT–CHUK–NFKBIA–NFKB signaling pathway. ( A ) BFTC905 cells were treated with ABT-751 (0.6 μM) for 24 h and the levels of the components of SKP2 E3 ligase (CDC20 and FZR1 proteins) were determined by immunoblots. ( B ) The cells were pretreated with 4 μM of MG132, a proteasome inhibitor, for 6 h, then treated with a specified concentration of ABT-751 (BFTC905, 0.6 μM and J82, 0.7 μM) for another 18 h. Next, nuclear/cytosol fractionation along with immunoblot analysis were performed. ( C ) The mRNA levels of SKP2 , TP 53, CDKN 1 A , CDKN 1 B , RB 1, E 2 F 1 and TFDP 1 were analyzed by quantitative RT-PCR. ( D ) The expression levels of the SKP2 gene regulator-associated proteins were determined by immunoblots in BFTC905 and J82 cells. Cells were treated with a specified concentration of ABT-751 (BFTC905, 0.6 μM and J82, 0.7 μM) for 24 h, ( E ) AKT1 kinase activity via a co-immunoprecipitation assay and ( F ) nuclear/cytosol fractionation along with immunoblot analysis were performed. ( G ) The cells were treated with a specified concentration of ABT-751 (BFTC905, 0.6 μM and J82, 0.7 μM) for 24 h, cell lysates were subjected to the co-immunoprecipitation assay with anti-RELA antibody. ( H ) Immunoblot analysis was used to compare the alterations of SKP2 -regulatory protein levels after treatment with a PI3K-AKT inhibitor, LY294002 (10 μM) for 2 h and ABT-751 (BFTC905, 0.6 μM and J82, 0.7 μM) for 24 h. ( I ) BFTC905 cells were treated with ABT-751 (0.6 μM) or LY294002 (10 μM) and SKP2 mRNA levels were analyzed by quantitative RT-PCR. ( J ) Transfection of a constitutive active AKT1 plasmid, pHRIG-AKT1, into BFTC905 cells for 24 h, suppressed AKT1-induced SKP2 mRNA levels. Relative protein levels were quantified using Image J software. The results are expressed as the means ± SD. Pan-actin, GAPDH and PARP1 served as loading, cytosolic and nuclear controls, respectively, in each gel. Statistical significance: * p

    Journal: International Journal of Molecular Sciences

    Article Title: ABT-751 Induces Multiple Anticancer Effects in Urinary Bladder Urothelial Carcinoma-Derived Cells: Highlighting the Induction of Cytostasis through the Inhibition of SKP2 at Both Transcriptional and Post-Translational Levels

    doi: 10.3390/ijms22020945

    Figure Lengend Snippet: ABT-751 inhibits SKP2 transcription and subsequent translation in UBUC cells through dysregulation of the AKT–CHUK–NFKBIA–NFKB signaling pathway. ( A ) BFTC905 cells were treated with ABT-751 (0.6 μM) for 24 h and the levels of the components of SKP2 E3 ligase (CDC20 and FZR1 proteins) were determined by immunoblots. ( B ) The cells were pretreated with 4 μM of MG132, a proteasome inhibitor, for 6 h, then treated with a specified concentration of ABT-751 (BFTC905, 0.6 μM and J82, 0.7 μM) for another 18 h. Next, nuclear/cytosol fractionation along with immunoblot analysis were performed. ( C ) The mRNA levels of SKP2 , TP 53, CDKN 1 A , CDKN 1 B , RB 1, E 2 F 1 and TFDP 1 were analyzed by quantitative RT-PCR. ( D ) The expression levels of the SKP2 gene regulator-associated proteins were determined by immunoblots in BFTC905 and J82 cells. Cells were treated with a specified concentration of ABT-751 (BFTC905, 0.6 μM and J82, 0.7 μM) for 24 h, ( E ) AKT1 kinase activity via a co-immunoprecipitation assay and ( F ) nuclear/cytosol fractionation along with immunoblot analysis were performed. ( G ) The cells were treated with a specified concentration of ABT-751 (BFTC905, 0.6 μM and J82, 0.7 μM) for 24 h, cell lysates were subjected to the co-immunoprecipitation assay with anti-RELA antibody. ( H ) Immunoblot analysis was used to compare the alterations of SKP2 -regulatory protein levels after treatment with a PI3K-AKT inhibitor, LY294002 (10 μM) for 2 h and ABT-751 (BFTC905, 0.6 μM and J82, 0.7 μM) for 24 h. ( I ) BFTC905 cells were treated with ABT-751 (0.6 μM) or LY294002 (10 μM) and SKP2 mRNA levels were analyzed by quantitative RT-PCR. ( J ) Transfection of a constitutive active AKT1 plasmid, pHRIG-AKT1, into BFTC905 cells for 24 h, suppressed AKT1-induced SKP2 mRNA levels. Relative protein levels were quantified using Image J software. The results are expressed as the means ± SD. Pan-actin, GAPDH and PARP1 served as loading, cytosolic and nuclear controls, respectively, in each gel. Statistical significance: * p

    Article Snippet: Quantitative Reverse Transcription-Polymerase Chain Reaction Quantitative reverse transcription-polymerase chain reaction (RT-PCR) was applied to quantify the mRNA expression levels of several genes using predesigned TaqMan® reagents from ThermoFisher (SKP2 : Hs01021864_m1 (59 bp); TP 53: Hs01034249_m1 (108 bp); CDKN 1A : Hs00355782_m1 (66 bp); CDKN 1B : Hs01597588_m1 (151 bp); RB 1: Hs01078066_m1 (72 bp); E 2F 1: Hs00153451_m1 (84 bp); TFDP 1: Hs00955488_g1 (102 bp); glyceraldehyde- 3-phosphate dehydrogenase (GAPDH ): Hs02758991_g1 (93 bp); CD 44 molecule, Indian blood group (CD 44): Hs01075861_m1 (70 bp); cadherin 1 (CDH 1): Hs01023895_m1 (80 bp); vimentin (VIM ): Hs00185584_m1 (73 bp); MTOR : Hs00234508_m1 (103 bp)) along with LightCycler® 96 System (Roche, Basel, Switzerland) and ΔΔCT calculation.

    Techniques: Western Blot, Concentration Assay, Fractionation, Quantitative RT-PCR, Expressing, Activity Assay, Co-Immunoprecipitation Assay, Transfection, Plasmid Preparation, Software