gene cdna Search Results


  • Logo
  • About
  • News
  • Press Release
  • Team
  • Advisors
  • Partners
  • Contact
  • Bioz Stars
  • Bioz vStars
  • 98
    Sino Biological sars cov 2 rbd
    The structure of CT-P59 Fab in complex with <t>SARS-CoV-2</t> RBD. a The overall structure of the CT-P59 Fab/SARS-CoV-2 RBD complex. The RBD domain is green for the core subdomain, and orange for RBM. The heavy and light chains of CT-P59 are magenta and yellow, respectively. b Superposition of the neutralizing antibodies in complex with RBD. RBD is shown as a surface model. CT-P59 is shown as a cartoon, and the other antibodies (CR3022: PDB 6XC3, PB2-2F6: PDB 7BWJ, REGN10933: PDB 6XDG) are shown as a ribbon model. The heavy and light chains of Fab are magenta and yellow, respectively. c Assignment of the epitope residues for RBD-targeting neutralizing antibodies with a distance cutoff of 4.5 Å. RBD residues interacting with ACE2 are highlighted in red. d The detailed interactions between the RBD and CDR loops of CT-P59. The interfaces between RBD and CDR H1/2 or H3 are shown in the top and bottom panels, respectively. The RBD domain is shown as a surface model with a semi-transparent representation. The CDR loops and interacting residues on the interfaces are shown in ribbons and sticks, respectively. The residues are colored as in a . Dashed lines indicate hydrogen bonds. Water molecules are shown as red spheres.
    Sars Cov 2 Rbd, supplied by Sino Biological, used in various techniques. Bioz Stars score: 98/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/sars cov 2 rbd/product/Sino Biological
    Average 98 stars, based on 1 article reviews
    Price from $9.99 to $1999.99
    sars cov 2 rbd - by Bioz Stars, 2021-09
    98/100 stars
      Buy from Supplier

    95
    Sino Biological sars cov 2 s1
    <t>SARS-CoV-2</t> infected mothers with male fetuses have lower plasma levels of SARS-CoV-2-specific antibodies. A. Plots showing maternal Spike-, RBD-, S1-, S2-, and N-specific maternal blood IgG2 levels. Female neonates of mothers with SARS-CoV-2 are shown as white bars with an orange border while male neonates are shown as orange shaded bars with orange border. Two-way ANOVA followed by post-hoc analyses were performed to determine significance. There was a main effect of fetal/neonatal sex on maternal IgG1 levels (p = 0.003). *p
    Sars Cov 2 S1, supplied by Sino Biological, used in various techniques. Bioz Stars score: 95/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/sars cov 2 s1/product/Sino Biological
    Average 95 stars, based on 1 article reviews
    Price from $9.99 to $1999.99
    sars cov 2 s1 - by Bioz Stars, 2021-09
    95/100 stars
      Buy from Supplier

    95
    Sino Biological egf
    <t>SARS-CoV-2</t> infected mothers with male fetuses have lower plasma levels of SARS-CoV-2-specific antibodies. A. Plots showing maternal Spike-, RBD-, S1-, S2-, and N-specific maternal blood IgG2 levels. Female neonates of mothers with SARS-CoV-2 are shown as white bars with an orange border while male neonates are shown as orange shaded bars with orange border. Two-way ANOVA followed by post-hoc analyses were performed to determine significance. There was a main effect of fetal/neonatal sex on maternal IgG1 levels (p = 0.003). *p
    Egf, supplied by Sino Biological, used in various techniques. Bioz Stars score: 95/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/egf/product/Sino Biological
    Average 95 stars, based on 1 article reviews
    Price from $9.99 to $1999.99
    egf - by Bioz Stars, 2021-09
    95/100 stars
      Buy from Supplier

    94
    Thermo Fisher complementary dna
    <t>SARS-CoV-2</t> infected mothers with male fetuses have lower plasma levels of SARS-CoV-2-specific antibodies. A. Plots showing maternal Spike-, RBD-, S1-, S2-, and N-specific maternal blood IgG2 levels. Female neonates of mothers with SARS-CoV-2 are shown as white bars with an orange border while male neonates are shown as orange shaded bars with orange border. Two-way ANOVA followed by post-hoc analyses were performed to determine significance. There was a main effect of fetal/neonatal sex on maternal IgG1 levels (p = 0.003). *p
    Complementary Dna, supplied by Thermo Fisher, used in various techniques. Bioz Stars score: 94/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/complementary dna/product/Thermo Fisher
    Average 94 stars, based on 1 article reviews
    Price from $9.99 to $1999.99
    complementary dna - by Bioz Stars, 2021-09
    94/100 stars
      Buy from Supplier

    Image Search Results


    The structure of CT-P59 Fab in complex with SARS-CoV-2 RBD. a The overall structure of the CT-P59 Fab/SARS-CoV-2 RBD complex. The RBD domain is green for the core subdomain, and orange for RBM. The heavy and light chains of CT-P59 are magenta and yellow, respectively. b Superposition of the neutralizing antibodies in complex with RBD. RBD is shown as a surface model. CT-P59 is shown as a cartoon, and the other antibodies (CR3022: PDB 6XC3, PB2-2F6: PDB 7BWJ, REGN10933: PDB 6XDG) are shown as a ribbon model. The heavy and light chains of Fab are magenta and yellow, respectively. c Assignment of the epitope residues for RBD-targeting neutralizing antibodies with a distance cutoff of 4.5 Å. RBD residues interacting with ACE2 are highlighted in red. d The detailed interactions between the RBD and CDR loops of CT-P59. The interfaces between RBD and CDR H1/2 or H3 are shown in the top and bottom panels, respectively. The RBD domain is shown as a surface model with a semi-transparent representation. The CDR loops and interacting residues on the interfaces are shown in ribbons and sticks, respectively. The residues are colored as in a . Dashed lines indicate hydrogen bonds. Water molecules are shown as red spheres.

    Journal: Nature Communications

    Article Title: A therapeutic neutralizing antibody targeting receptor binding domain of SARS-CoV-2 spike protein

    doi: 10.1038/s41467-020-20602-5

    Figure Lengend Snippet: The structure of CT-P59 Fab in complex with SARS-CoV-2 RBD. a The overall structure of the CT-P59 Fab/SARS-CoV-2 RBD complex. The RBD domain is green for the core subdomain, and orange for RBM. The heavy and light chains of CT-P59 are magenta and yellow, respectively. b Superposition of the neutralizing antibodies in complex with RBD. RBD is shown as a surface model. CT-P59 is shown as a cartoon, and the other antibodies (CR3022: PDB 6XC3, PB2-2F6: PDB 7BWJ, REGN10933: PDB 6XDG) are shown as a ribbon model. The heavy and light chains of Fab are magenta and yellow, respectively. c Assignment of the epitope residues for RBD-targeting neutralizing antibodies with a distance cutoff of 4.5 Å. RBD residues interacting with ACE2 are highlighted in red. d The detailed interactions between the RBD and CDR loops of CT-P59. The interfaces between RBD and CDR H1/2 or H3 are shown in the top and bottom panels, respectively. The RBD domain is shown as a surface model with a semi-transparent representation. The CDR loops and interacting residues on the interfaces are shown in ribbons and sticks, respectively. The residues are colored as in a . Dashed lines indicate hydrogen bonds. Water molecules are shown as red spheres.

    Article Snippet: For the production of SARS-CoV-2 RBD, DNA encoding the SARS-CoV-2 S protein RBD (YP_009724390.1: Arg319-Asn536) with a polyhistidine tag at the C-terminus was cloned into the MarEx vector and transiently expressed in CHO cells.

    Techniques:

    Virus titration and quantitation in the lower respiratory tract of animal models. Three ferrets per group were euthanized at 3 and 7 dpi, and lungs were collected to measure viral titers ( a ) and the number of viral RNA copies ( d ). Golden Syrian hamsters ( n = 12/group) were challenged intranasally with 6.4 × 10 4 PFU/80 μL of SARS-CoV-2. Vehicle and 15, 30, 60, and 90 mg/kg of CT-P59 were intraperitoneally administered 24 h after virus inoculation. Four animals were euthanized for virus titration ( b ) and quantitation of viral RNA copies ( e ) from each group at 3 and 5 dpi. Rhesus monkeys (control n = 3, 45 mg/kg n = 2, 90 mg/kg n = 3) were infected with 10 6.4 TCID 50 /ml of SARS-CoV-2 via in a combination of intranasal (0.5 ml), intratracheal (4 ml), ocular (0.25 ml/eye), and oral (1 ml) routes. Vehicle, 45 mg/kg and 90 mg/kg of CT-P59 were administered intravenously after 24 h of virus infection. All rhesus monkeys were euthanized at 6 dpi, and lungs were collected to measure viral titers ( c ) and the number of viral RNA copies ( f ). Viral titers in the lung were determined by TCID 50 assessment in Vero cells and viral RNA copy number measurement using qRT-PCR. Viral titers and RNA copy numbers are shown as mean value + /− SEM and titers below the limit of detection are shown as 0.8 log 10 TCID 50 /ml or 0.3 log 10 viral RNA copies/ml (dashed lines). Asterisks indicate statistical significance between the control and each group as determined by two-way ANOVA and subsequent Dunnett’s test. * P = 0.0309, ** P = 0.0131, and *** P

    Journal: Nature Communications

    Article Title: A therapeutic neutralizing antibody targeting receptor binding domain of SARS-CoV-2 spike protein

    doi: 10.1038/s41467-020-20602-5

    Figure Lengend Snippet: Virus titration and quantitation in the lower respiratory tract of animal models. Three ferrets per group were euthanized at 3 and 7 dpi, and lungs were collected to measure viral titers ( a ) and the number of viral RNA copies ( d ). Golden Syrian hamsters ( n = 12/group) were challenged intranasally with 6.4 × 10 4 PFU/80 μL of SARS-CoV-2. Vehicle and 15, 30, 60, and 90 mg/kg of CT-P59 were intraperitoneally administered 24 h after virus inoculation. Four animals were euthanized for virus titration ( b ) and quantitation of viral RNA copies ( e ) from each group at 3 and 5 dpi. Rhesus monkeys (control n = 3, 45 mg/kg n = 2, 90 mg/kg n = 3) were infected with 10 6.4 TCID 50 /ml of SARS-CoV-2 via in a combination of intranasal (0.5 ml), intratracheal (4 ml), ocular (0.25 ml/eye), and oral (1 ml) routes. Vehicle, 45 mg/kg and 90 mg/kg of CT-P59 were administered intravenously after 24 h of virus infection. All rhesus monkeys were euthanized at 6 dpi, and lungs were collected to measure viral titers ( c ) and the number of viral RNA copies ( f ). Viral titers in the lung were determined by TCID 50 assessment in Vero cells and viral RNA copy number measurement using qRT-PCR. Viral titers and RNA copy numbers are shown as mean value + /− SEM and titers below the limit of detection are shown as 0.8 log 10 TCID 50 /ml or 0.3 log 10 viral RNA copies/ml (dashed lines). Asterisks indicate statistical significance between the control and each group as determined by two-way ANOVA and subsequent Dunnett’s test. * P = 0.0309, ** P = 0.0131, and *** P

    Article Snippet: For the production of SARS-CoV-2 RBD, DNA encoding the SARS-CoV-2 S protein RBD (YP_009724390.1: Arg319-Asn536) with a polyhistidine tag at the C-terminus was cloned into the MarEx vector and transiently expressed in CHO cells.

    Techniques: Titration, Quantitation Assay, Infection, Quantitative RT-PCR

    In vivo efficacy of CT-P59 in the upper respiratory tract in an animal model. Female ferrets ( n = 6/group), and rhesus monkeys (three control; two 45 mg/kg; three 90 mg/kg) were challenged with 10 5.8 TCID 50 /ml and 10 6.4 TCID 50 of SARS-CoV-2, respectively. Control (ferrets: 30 mg/kg of human IgG isotype and rhesus monkeys: vehicle) and CT-P59 (ferrets: 3, and 30 mg/kg, rhesus monkeys: 45, and 90 mg/kg) were administered intravenously after 24 h of virus inoculation, respectively. To compare the efficacy of CT-P59, remdesivir (18 mg/kg per ferret) was administered daily via oral gavage after 24 h of virus inoculation in ferrets for 5 days. To detect viral load in the upper respiratory tract, nasal wash specimens were collected from ferrets at 2, 4, and 6 dpi, and nose and throat swab specimens from monkeys were collected daily up to 6 dpi. Virus titers (TCID 50 ) were measured in nasal wash/swab and throat swabs specimens from each group of ( a ) ferrets and, c , d rhesus monkeys. The viral RNA copy numbers were measured in nasal washes from ( b ) ferrets. Viral titers and RNA copy numbers are shown as mean values + /− SEM and titers below the limit of detection are shown as 0.8 log 10 TCID 50 /ml or 0.3 log 10 viral RNA copies/ml (dashed lines). The asterisks and daggers indicate significance between the control and each group as determined by two-way ANOVA and subsequent Dunnett’s test. * P = 0.0001 and ** P

    Journal: Nature Communications

    Article Title: A therapeutic neutralizing antibody targeting receptor binding domain of SARS-CoV-2 spike protein

    doi: 10.1038/s41467-020-20602-5

    Figure Lengend Snippet: In vivo efficacy of CT-P59 in the upper respiratory tract in an animal model. Female ferrets ( n = 6/group), and rhesus monkeys (three control; two 45 mg/kg; three 90 mg/kg) were challenged with 10 5.8 TCID 50 /ml and 10 6.4 TCID 50 of SARS-CoV-2, respectively. Control (ferrets: 30 mg/kg of human IgG isotype and rhesus monkeys: vehicle) and CT-P59 (ferrets: 3, and 30 mg/kg, rhesus monkeys: 45, and 90 mg/kg) were administered intravenously after 24 h of virus inoculation, respectively. To compare the efficacy of CT-P59, remdesivir (18 mg/kg per ferret) was administered daily via oral gavage after 24 h of virus inoculation in ferrets for 5 days. To detect viral load in the upper respiratory tract, nasal wash specimens were collected from ferrets at 2, 4, and 6 dpi, and nose and throat swab specimens from monkeys were collected daily up to 6 dpi. Virus titers (TCID 50 ) were measured in nasal wash/swab and throat swabs specimens from each group of ( a ) ferrets and, c , d rhesus monkeys. The viral RNA copy numbers were measured in nasal washes from ( b ) ferrets. Viral titers and RNA copy numbers are shown as mean values + /− SEM and titers below the limit of detection are shown as 0.8 log 10 TCID 50 /ml or 0.3 log 10 viral RNA copies/ml (dashed lines). The asterisks and daggers indicate significance between the control and each group as determined by two-way ANOVA and subsequent Dunnett’s test. * P = 0.0001 and ** P

    Article Snippet: For the production of SARS-CoV-2 RBD, DNA encoding the SARS-CoV-2 S protein RBD (YP_009724390.1: Arg319-Asn536) with a polyhistidine tag at the C-terminus was cloned into the MarEx vector and transiently expressed in CHO cells.

    Techniques: In Vivo, Animal Model

    CT-P59 can effectively neutralize SARS-CoV-2 in vitro by blocking RBD-ACE2 binding. a Serial twofold-diluted CT-P59 were incubated with SARS-CoV-2 live viruses; wild type (blue) and D614G (red). The mixture was added to VeroE6 cells. After 2–3 days of incubation, the neutralization activity was evaluated by counting plaques. Two independent experiments were performed in duplicate. b SARS-CoV-2 RBD immobilized on biosensor was saturated with CT-P59. Then, CT-P59 flowed over the biosensor surface in the presence (red) or absence (blue) of the ACE2 receptor. As a positive control, the buffer was loaded onto SARS-CoV-2 RBD immobilized biosensor, and ACE2 flowed over the biosensor surface (black).

    Journal: Nature Communications

    Article Title: A therapeutic neutralizing antibody targeting receptor binding domain of SARS-CoV-2 spike protein

    doi: 10.1038/s41467-020-20602-5

    Figure Lengend Snippet: CT-P59 can effectively neutralize SARS-CoV-2 in vitro by blocking RBD-ACE2 binding. a Serial twofold-diluted CT-P59 were incubated with SARS-CoV-2 live viruses; wild type (blue) and D614G (red). The mixture was added to VeroE6 cells. After 2–3 days of incubation, the neutralization activity was evaluated by counting plaques. Two independent experiments were performed in duplicate. b SARS-CoV-2 RBD immobilized on biosensor was saturated with CT-P59. Then, CT-P59 flowed over the biosensor surface in the presence (red) or absence (blue) of the ACE2 receptor. As a positive control, the buffer was loaded onto SARS-CoV-2 RBD immobilized biosensor, and ACE2 flowed over the biosensor surface (black).

    Article Snippet: For the production of SARS-CoV-2 RBD, DNA encoding the SARS-CoV-2 S protein RBD (YP_009724390.1: Arg319-Asn536) with a polyhistidine tag at the C-terminus was cloned into the MarEx vector and transiently expressed in CHO cells.

    Techniques: In Vitro, Blocking Assay, Binding Assay, Incubation, Neutralization, Activity Assay, Positive Control

    No evidence of ADE caused by CT-P59 bound to SARS-CoV-2 in permissive cells and Fc receptor (FcR)-bearing cells. a FcR-independent ADE. SARS-CoV-2 was mixed with a wide range of antibodies; CT-P59 (red circle), CR3022 (blue square), and CT-P27 (black triangle). VeroE6 was infected with a virus–antibody complex. The virus titers were determined by optical density (OD) using an anti-nucleocapsid antibody. b FcγR II-dependent ADE. In vitro ADE assay was performed as described in ( a ) except Raji cells. c FcγR I II-dependent ADE. In vitro ADE assay was carried out as described in ( a ), except U937 cells. The experiments were performed in triplicates (CR3022 and CT-P27) or in quadruplicates (CT-P59). Average and standard deviation of virus titers are depicted as dot and error bar, respectively.

    Journal: Nature Communications

    Article Title: A therapeutic neutralizing antibody targeting receptor binding domain of SARS-CoV-2 spike protein

    doi: 10.1038/s41467-020-20602-5

    Figure Lengend Snippet: No evidence of ADE caused by CT-P59 bound to SARS-CoV-2 in permissive cells and Fc receptor (FcR)-bearing cells. a FcR-independent ADE. SARS-CoV-2 was mixed with a wide range of antibodies; CT-P59 (red circle), CR3022 (blue square), and CT-P27 (black triangle). VeroE6 was infected with a virus–antibody complex. The virus titers were determined by optical density (OD) using an anti-nucleocapsid antibody. b FcγR II-dependent ADE. In vitro ADE assay was performed as described in ( a ) except Raji cells. c FcγR I II-dependent ADE. In vitro ADE assay was carried out as described in ( a ), except U937 cells. The experiments were performed in triplicates (CR3022 and CT-P27) or in quadruplicates (CT-P59). Average and standard deviation of virus titers are depicted as dot and error bar, respectively.

    Article Snippet: For the production of SARS-CoV-2 RBD, DNA encoding the SARS-CoV-2 S protein RBD (YP_009724390.1: Arg319-Asn536) with a polyhistidine tag at the C-terminus was cloned into the MarEx vector and transiently expressed in CHO cells.

    Techniques: Infection, In Vitro, Standard Deviation

    SARS-CoV-2 infected mothers with male fetuses have lower plasma levels of SARS-CoV-2-specific antibodies. A. Plots showing maternal Spike-, RBD-, S1-, S2-, and N-specific maternal blood IgG2 levels. Female neonates of mothers with SARS-CoV-2 are shown as white bars with an orange border while male neonates are shown as orange shaded bars with orange border. Two-way ANOVA followed by post-hoc analyses were performed to determine significance. There was a main effect of fetal/neonatal sex on maternal IgG1 levels (p = 0.003). *p

    Journal: bioRxiv

    Article Title: Sexually dimorphic placental responses to maternal SARS-CoV-2 infection

    doi: 10.1101/2021.03.29.437516

    Figure Lengend Snippet: SARS-CoV-2 infected mothers with male fetuses have lower plasma levels of SARS-CoV-2-specific antibodies. A. Plots showing maternal Spike-, RBD-, S1-, S2-, and N-specific maternal blood IgG2 levels. Female neonates of mothers with SARS-CoV-2 are shown as white bars with an orange border while male neonates are shown as orange shaded bars with orange border. Two-way ANOVA followed by post-hoc analyses were performed to determine significance. There was a main effect of fetal/neonatal sex on maternal IgG1 levels (p = 0.003). *p

    Article Snippet: SARS-CoV-2 S1 (Sino Biological), SARS-CoV-2 S2 (Sino Biological), pertussis pertactin (List Reagents) and a mix of HA A/Michigan/45/2015 (H1N1), HA A/Singapore/INFIMH-16-0019/2016 (H3N2), B/Phuket/3073/2013 (Immunetech).

    Techniques: Infection

    SARS-CoV-2 infected mothers with male fetuses demonstrate reduced placental transfer of SARS-CoV-2 antibodies compared to those with female fetuses. A. Dot plots showing relative Spike-, RBD-, S1-, S2-, N-, and influenza (HA)-specific maternal blood (M) and cord blood (C) titers of IgG1. Female neonates of SARS-CoV-2 negative mothers are shown in light blue, female neonates of SARS-CoV-2 positive mothers are shown in light orange. Males born to SARS-CoV-2 negative mothers are shown in dark blue, and males born to SARS-CoV-2 positive mothers are shown in dark orange. Units are Mean Fluorescence Intensity or MFI. All values reflect PBS background correction. Wilcoxon matched-pairs signed rank test was performed to determine significance. * p

    Journal: bioRxiv

    Article Title: Sexually dimorphic placental responses to maternal SARS-CoV-2 infection

    doi: 10.1101/2021.03.29.437516

    Figure Lengend Snippet: SARS-CoV-2 infected mothers with male fetuses demonstrate reduced placental transfer of SARS-CoV-2 antibodies compared to those with female fetuses. A. Dot plots showing relative Spike-, RBD-, S1-, S2-, N-, and influenza (HA)-specific maternal blood (M) and cord blood (C) titers of IgG1. Female neonates of SARS-CoV-2 negative mothers are shown in light blue, female neonates of SARS-CoV-2 positive mothers are shown in light orange. Males born to SARS-CoV-2 negative mothers are shown in dark blue, and males born to SARS-CoV-2 positive mothers are shown in dark orange. Units are Mean Fluorescence Intensity or MFI. All values reflect PBS background correction. Wilcoxon matched-pairs signed rank test was performed to determine significance. * p

    Article Snippet: SARS-CoV-2 S1 (Sino Biological), SARS-CoV-2 S2 (Sino Biological), pertussis pertactin (List Reagents) and a mix of HA A/Michigan/45/2015 (H1N1), HA A/Singapore/INFIMH-16-0019/2016 (H3N2), B/Phuket/3073/2013 (Immunetech).

    Techniques: Infection, Fluorescence

    Sexually dimorphic regulation of Fc receptor gene expression, protein expression, and colocalization. (A-C) RTqPCR analyses of male or female placental expression of FCGRT (A) , FCGR1 (B), and FCGR3A (C) in placental biopsies from SARS-CoV-2 negative (blue) or SARS-CoV-2 positive (orange) pregnancies. Expression levels shown are relative to reference gene YWHAZ . (D-F) Representative immunoblots and quantification of fetal female or fetal male expression of FcRn (A), FCγR1 (B), and FCγR3 (C) in placental biopsies from SARS-CoV-2 negative (blue) or SARS-CoV-2 positive (orange) pregnancies. Neg and Pos on western blot designates SARS-CoV-2 negative and positive pregnancies. (G) Placental tissue sections from SARS-CoV-2 + and SARS-CoV-2 – mothers were stained for FCγR3 (red), FcRn (purple), and placental alkaline phosphatase (PLAP, green), a trophoblast marker, and DAPI (blue). (H) Box-and-whisker plots showing FCγR3/FcRn co-localization in placental villi. (I) Placental tissue sections from SARS-CoV-2 + and SARS-CoV-2 – mothers were stained for FCγR1 (purple), FcRn (red), and placental alkaline phosphatase (PLAP, green), a trophoblast marker, and DAPI (blue). (J) Box-and-whisker plots showing (J) FCγR1/FcRn or (K) FCγR2/FcRn co-localization in placental villi. Two-way ANOVA followed by Bonferroni’s post-hoc analyses were performed to determine significance. * p

    Journal: bioRxiv

    Article Title: Sexually dimorphic placental responses to maternal SARS-CoV-2 infection

    doi: 10.1101/2021.03.29.437516

    Figure Lengend Snippet: Sexually dimorphic regulation of Fc receptor gene expression, protein expression, and colocalization. (A-C) RTqPCR analyses of male or female placental expression of FCGRT (A) , FCGR1 (B), and FCGR3A (C) in placental biopsies from SARS-CoV-2 negative (blue) or SARS-CoV-2 positive (orange) pregnancies. Expression levels shown are relative to reference gene YWHAZ . (D-F) Representative immunoblots and quantification of fetal female or fetal male expression of FcRn (A), FCγR1 (B), and FCγR3 (C) in placental biopsies from SARS-CoV-2 negative (blue) or SARS-CoV-2 positive (orange) pregnancies. Neg and Pos on western blot designates SARS-CoV-2 negative and positive pregnancies. (G) Placental tissue sections from SARS-CoV-2 + and SARS-CoV-2 – mothers were stained for FCγR3 (red), FcRn (purple), and placental alkaline phosphatase (PLAP, green), a trophoblast marker, and DAPI (blue). (H) Box-and-whisker plots showing FCγR3/FcRn co-localization in placental villi. (I) Placental tissue sections from SARS-CoV-2 + and SARS-CoV-2 – mothers were stained for FCγR1 (purple), FcRn (red), and placental alkaline phosphatase (PLAP, green), a trophoblast marker, and DAPI (blue). (J) Box-and-whisker plots showing (J) FCγR1/FcRn or (K) FCγR2/FcRn co-localization in placental villi. Two-way ANOVA followed by Bonferroni’s post-hoc analyses were performed to determine significance. * p

    Article Snippet: SARS-CoV-2 S1 (Sino Biological), SARS-CoV-2 S2 (Sino Biological), pertussis pertactin (List Reagents) and a mix of HA A/Michigan/45/2015 (H1N1), HA A/Singapore/INFIMH-16-0019/2016 (H3N2), B/Phuket/3073/2013 (Immunetech).

    Techniques: Expressing, Western Blot, Staining, Marker, Whisker Assay

    Male-specific upregulation of interferon stimulated genes in placentas exposed to maternal SARS-CoV-2 infection. (A) Interferon stimulated gene pathway diagram. Production of interferon stimulated genes (ISGs) of interest can occur through activation of Type I IFN (left) or Type III IFN (right). Image created using Biorender. (B-G) RTqPCR analyses of male or female placental expression of (B) IFI6, (C) CXCL10, (D) OAS1 , (E) CCL2 , (F) MX1 , and (G) IL10 in placental biopsies from SARS-CoV-2 negative (blue) or SARS-CoV-2 positive (orange) pregnancies. Expression levels shown are relative to reference gene YWHAZ. (H-I) Representative immunohistochemistry images and quantification of CD163-positive cells in placental slices from SARS-CoV-2 negative (blue) or SARS-CoV-2 positive (orange) pregnancies. Two-way ANOVA followed by Bonferroni’s post-hoc analyses were performed to determine significance. * p

    Journal: bioRxiv

    Article Title: Sexually dimorphic placental responses to maternal SARS-CoV-2 infection

    doi: 10.1101/2021.03.29.437516

    Figure Lengend Snippet: Male-specific upregulation of interferon stimulated genes in placentas exposed to maternal SARS-CoV-2 infection. (A) Interferon stimulated gene pathway diagram. Production of interferon stimulated genes (ISGs) of interest can occur through activation of Type I IFN (left) or Type III IFN (right). Image created using Biorender. (B-G) RTqPCR analyses of male or female placental expression of (B) IFI6, (C) CXCL10, (D) OAS1 , (E) CCL2 , (F) MX1 , and (G) IL10 in placental biopsies from SARS-CoV-2 negative (blue) or SARS-CoV-2 positive (orange) pregnancies. Expression levels shown are relative to reference gene YWHAZ. (H-I) Representative immunohistochemistry images and quantification of CD163-positive cells in placental slices from SARS-CoV-2 negative (blue) or SARS-CoV-2 positive (orange) pregnancies. Two-way ANOVA followed by Bonferroni’s post-hoc analyses were performed to determine significance. * p

    Article Snippet: SARS-CoV-2 S1 (Sino Biological), SARS-CoV-2 S2 (Sino Biological), pertussis pertactin (List Reagents) and a mix of HA A/Michigan/45/2015 (H1N1), HA A/Singapore/INFIMH-16-0019/2016 (H3N2), B/Phuket/3073/2013 (Immunetech).

    Techniques: Infection, Activation Assay, Expressing, Immunohistochemistry

    No effect of maternal SARS-CoV-2 infection on expression or localization of FCγR2. (A-C) qPCR analyses of fetal male or fetal female expression of ( A ) FCGR3B, ( B ) FCGR2A, and ( C ) FCGR2B in placental biopsies from SARS-CoV-2 negative (blue) or SARS-CoV-2 positive (orange) pregnancies. Expression levels shown are relative to reference gene YWHAZ . (D) Representative immunoblots and quantification of FCγR2 in female or male placental biopsies from mothers testing negative (blue) or positive (orange) for SARS-CoV-2. (E) Placental tissue sections from SARS-CoV-2 + and SARS-CoV-2 – mothers were stained for FCγR2 (purple), FcRn (red), and placental alkaline phosphatase (PLAP, green), a trophoblast marker, and DAPI (blue). (F) Box-and-whisker plots showing FCγR2/FcRn co-localization in placental villi. Two-way ANOVA followed by post-hoc analyses were performed to determine significance. * p

    Journal: bioRxiv

    Article Title: Sexually dimorphic placental responses to maternal SARS-CoV-2 infection

    doi: 10.1101/2021.03.29.437516

    Figure Lengend Snippet: No effect of maternal SARS-CoV-2 infection on expression or localization of FCγR2. (A-C) qPCR analyses of fetal male or fetal female expression of ( A ) FCGR3B, ( B ) FCGR2A, and ( C ) FCGR2B in placental biopsies from SARS-CoV-2 negative (blue) or SARS-CoV-2 positive (orange) pregnancies. Expression levels shown are relative to reference gene YWHAZ . (D) Representative immunoblots and quantification of FCγR2 in female or male placental biopsies from mothers testing negative (blue) or positive (orange) for SARS-CoV-2. (E) Placental tissue sections from SARS-CoV-2 + and SARS-CoV-2 – mothers were stained for FCγR2 (purple), FcRn (red), and placental alkaline phosphatase (PLAP, green), a trophoblast marker, and DAPI (blue). (F) Box-and-whisker plots showing FCγR2/FcRn co-localization in placental villi. Two-way ANOVA followed by post-hoc analyses were performed to determine significance. * p

    Article Snippet: SARS-CoV-2 S1 (Sino Biological), SARS-CoV-2 S2 (Sino Biological), pertussis pertactin (List Reagents) and a mix of HA A/Michigan/45/2015 (H1N1), HA A/Singapore/INFIMH-16-0019/2016 (H3N2), B/Phuket/3073/2013 (Immunetech).

    Techniques: Infection, Expressing, Real-time Polymerase Chain Reaction, Western Blot, Staining, Marker, Whisker Assay