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GenScript corporation
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Image Search Results
Journal: International Journal of Molecular Sciences
Article Title: Production of Two Novel Methoxy-Isoflavones from Biotransformation of 8-Hydroxydaidzein by Recombinant Escherichia coli Expressing O -Methyltransferase SpOMT2884 from Streptomyces peucetius
doi: 10.3390/ijms161126070
Figure Lengend Snippet: Construction and expression of SpOMT2884 in E. coli . ( A ) Map of the expression vector pETDuet-SpOMT2884; ( B ) Sodium dodecyl sulfate polyacrylamide gel electrophoresis (SDS-PAGE) of recombinant E. coli harboring pETDuet-SpOMT2884. Crude proteins from whole cell lysis with 0 h induction (lane 1), 4 h induction (lane 2), and 8 h induction (lane 3) were separated with SDS-PAGE. Arrows indicate the expressed SpOMT2884. M represents the molecular weight markers.
Article Snippet: The
Techniques: Expressing, Plasmid Preparation, Polyacrylamide Gel Electrophoresis, SDS Page, Recombinant, Lysis, Molecular Weight
Journal: International Journal of Molecular Sciences
Article Title: Production of Two Novel Methoxy-Isoflavones from Biotransformation of 8-Hydroxydaidzein by Recombinant Escherichia coli Expressing O -Methyltransferase SpOMT2884 from Streptomyces peucetius
doi: 10.3390/ijms161126070
Figure Lengend Snippet: Ultra-performance liquid chromatography (UPLC) profiles of 8-hydroxydaidzein standard ( A ) and fermentation broth (( B ): 0 h induction; ( C ): 24 h induction) of recombinant E. coli expressing SpOMT2884. The recombinant strain was cultivated at 37 °C in shacking flasks with LeMaster and Richards minimal medium (LR medium) containing 100 μM 8-hydroxydaidzein. After induction with 0.5 mM isopropyl-β- d -thiogalactopyranoside (IPTG), aliquot samples of the fermentation were collected and analyzed with UPLC. The detailed protocols for fermentation and UPLC are described in Materials and Methods.
Article Snippet: The
Techniques: Liquid Chromatography, Recombinant, Expressing
Journal: International Journal of Molecular Sciences
Article Title: Production of Two Novel Methoxy-Isoflavones from Biotransformation of 8-Hydroxydaidzein by Recombinant Escherichia coli Expressing O -Methyltransferase SpOMT2884 from Streptomyces peucetius
doi: 10.3390/ijms161126070
Figure Lengend Snippet: Diagram of the biotransformation of 8-hydroxydaidzein by the recombinant E. coli expressing SpOMT2884.
Article Snippet: The
Techniques: Recombinant, Expressing