Journal: Molecular & Cellular Proteomics : MCP
Article Title: Systematic Analysis of the Phosphoproteome and Kinase-substrate Networks in the Mouse Testis *
Figure Lengend Snippet: The phosphorylation level of PLK1 and inhibition assay of the PLKs in GC2 cells. A , The expression and pT210 levels of PLK1 in testes and mixed tissues of different developmental stages were analyzed by Western blot, with β actin as a loading control. Mixed tissues is the pooling of equal amount of brain, brown fat, heart, liver, lung, kidney, pancreas, and spleen from 3-week or 8-week mice. The distribution patterns of GC2 cells in different phases of the cell cycle were compared after treatment with BI2536 and/or OA for 2 h B , 4 h C , and 6 h D .
Article Snippet: Mouse GC2 cells (ATCC catalog number CRL-2196, Manassas, VA, USA) were cultured for 16 h at 37 °C in 5% CO2 in DMEM culture medium supplemented with 10% fetal bovine serum, 0.5% penicillin and streptomycin.
Techniques: Inhibition, Expressing, Western Blot, Mouse Assay