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Image Search Results
Journal: Thoracic Cancer
Article Title: Knockdown of circ‐RAD23B inhibits non‐small cell lung cancer progression via the miR ‐142‐3p/ MAP4K3 axis
doi: 10.1111/1759-7714.14319
Figure Lengend Snippet: The circ‐RAD23B/miR‐142‐3p/MAP4K3 axis regulated the MAPK signaling pathway. (a) The protein level of MAP4K3 in H1299 and A549 cells transfected with si‐circ‐RAD23B‐1 or si‐circ‐RAD23B‐1 + miR‐142‐3p inhibitor was detected by western blot. (b and c) The protein levels of p‐ERK1/2, p‐JNK and p‐p38 in H1299 and A549 cells transfected with si‐circ‐RAD23B‐1 or si‐circ‐RAD23B‐1 + miR‐142‐3p inhibitor was detected by western blot. * p < 0.05
Article Snippet: The membranes were next incubated with the primary and secondary antibodies, including anti‐MAP4K3 (#92427; Cell Signaling Technology), antiproliferating cell nuclear antigen (anti‐PCNA; ab18197; Abcam), anti‐Bcl‐2‐associated X protein (anti‐Bax; ab182733), anti‐ERK1/2 (ab17942; Abcam), anti‐p‐ERK1/2 (ab223500; Abcam), anti‐JNK (AB208035; Abcam), anti‐p‐JNK (ab76572; Abcam), anti‐p38 (ab32142; Abcam),
Techniques: Transfection, Western Blot