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  • 93
    Bio-Techne corporation gap27
    Gap27, supplied by Bio-Techne corporation, used in various techniques. Bioz Stars score: 93/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/gap27/product/Bio-Techne corporation
    Average 93 stars, based on 1 article reviews
    Price from $9.99 to $1999.99
    gap27 - by Bioz Stars, 2024-05
    93/100 stars
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    86
    AnaSpec gap 27
    Gap 27, supplied by AnaSpec, used in various techniques. Bioz Stars score: 86/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/gap 27/product/AnaSpec
    Average 86 stars, based on 1 article reviews
    Price from $9.99 to $1999.99
    gap 27 - by Bioz Stars, 2024-05
    86/100 stars
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    86
    Zealand Pharma gap27
    <t>Gap27</t> (100 μM) influences the speed of Juvenile Foreskin Fibroblasts (JFF) cell migration. ( A ) time-lapse migration data of JFF cells; ( B ) Average cell velocity of JFF cells; ( C ) Cell velocity of JFF cells at the leading edge of the scrape wound; ( D ) Cell velocity of JFF cells 0–50 μm behind the wound edge; ( E ) Cell velocity of JFF cells 50–100 μm behind the wound edge. n = 18 cells were tracked in total with 6 cells from each specific area. *** p < 0.005.
    Gap27, supplied by Zealand Pharma, used in various techniques. Bioz Stars score: 86/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/gap27/product/Zealand Pharma
    Average 86 stars, based on 1 article reviews
    Price from $9.99 to $1999.99
    gap27 - by Bioz Stars, 2024-05
    86/100 stars
      Buy from Supplier

    86
    Millipore gap27
    <t>Gap27</t> (100 μM) influences the speed of Juvenile Foreskin Fibroblasts (JFF) cell migration. ( A ) time-lapse migration data of JFF cells; ( B ) Average cell velocity of JFF cells; ( C ) Cell velocity of JFF cells at the leading edge of the scrape wound; ( D ) Cell velocity of JFF cells 0–50 μm behind the wound edge; ( E ) Cell velocity of JFF cells 50–100 μm behind the wound edge. n = 18 cells were tracked in total with 6 cells from each specific area. *** p < 0.005.
    Gap27, supplied by Millipore, used in various techniques. Bioz Stars score: 86/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/gap27/product/Millipore
    Average 86 stars, based on 1 article reviews
    Price from $9.99 to $1999.99
    gap27 - by Bioz Stars, 2024-05
    86/100 stars
      Buy from Supplier

    86
    LifeTein Inc gap27
    ( A ) The effect of <t>Gap27</t> on PI3K signaling. U87MG cells were treated with 100 μM TMZ or 100 μM Gap27. ( B ) ATP release from Cx43-high/MGMT-deficient/TMZ-resistant SF295 cells. Cells were treated with 100 μM αCT1. Culture media were collected at different time points. ATP was measured using a colorimetric assay. Oneway ANOVA was used to determine statistical significance. ( C ) Glutamate release in Cx43-low/MGMT-deficient/TMZ-sensitive LN229 or Cx43-high/MGMT-deficient/TMZ-resistant LN229/GSC cells. Cells were treated with 100 μM TMZ and/or 100 μM αCT1. Glutamate in culture media was determined using a colorimetric assay. ( D ) ATP release in LN229 and LN229/GSC cells. ( E ) ATP within SF295 cells. ( F ) Glutamate within LN229 and LN229/GSC cells. GSC: glioblastoma stem cells.
    Gap27, supplied by LifeTein Inc, used in various techniques. Bioz Stars score: 86/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/gap27/product/LifeTein Inc
    Average 86 stars, based on 1 article reviews
    Price from $9.99 to $1999.99
    gap27 - by Bioz Stars, 2024-05
    86/100 stars
      Buy from Supplier

    Image Search Results


    Gap27 (100 μM) influences the speed of Juvenile Foreskin Fibroblasts (JFF) cell migration. ( A ) time-lapse migration data of JFF cells; ( B ) Average cell velocity of JFF cells; ( C ) Cell velocity of JFF cells at the leading edge of the scrape wound; ( D ) Cell velocity of JFF cells 0–50 μm behind the wound edge; ( E ) Cell velocity of JFF cells 50–100 μm behind the wound edge. n = 18 cells were tracked in total with 6 cells from each specific area. *** p < 0.005.

    Journal: International Journal of Molecular Sciences

    Article Title: The Connexin Mimetic Peptide Gap27 and Cx43-Knockdown Reveal Differential Roles for Connexin43 in Wound Closure Events in Skin Model Systems

    doi: 10.3390/ijms19020604

    Figure Lengend Snippet: Gap27 (100 μM) influences the speed of Juvenile Foreskin Fibroblasts (JFF) cell migration. ( A ) time-lapse migration data of JFF cells; ( B ) Average cell velocity of JFF cells; ( C ) Cell velocity of JFF cells at the leading edge of the scrape wound; ( D ) Cell velocity of JFF cells 0–50 μm behind the wound edge; ( E ) Cell velocity of JFF cells 50–100 μm behind the wound edge. n = 18 cells were tracked in total with 6 cells from each specific area. *** p < 0.005.

    Article Snippet: For the purpose of this study, Gap27 (MW 1305) (Zealand Pharma, Glostrop, Denmark) was used in aqueous solution at doses ranging from 1–100 μM for 15 min–48 h depending on experimental requirements.

    Techniques: Migration

    Gap27 and SiRNA targeted to Cx43 have differential effects on scrape wound closure rates in skin cells. Dose response of Gap27 in AK cells ( A ); SiRNA targeted to Cx43 and 100 nM Gap27 enhance scrape wound closure in AK ( B ) and JFF cells ( C ); Gap27 does not enhance cell migration rates in ADF or HNDF cells ( D – F ). n = 3, ** p < 0.01; *** p < 0.005.

    Journal: International Journal of Molecular Sciences

    Article Title: The Connexin Mimetic Peptide Gap27 and Cx43-Knockdown Reveal Differential Roles for Connexin43 in Wound Closure Events in Skin Model Systems

    doi: 10.3390/ijms19020604

    Figure Lengend Snippet: Gap27 and SiRNA targeted to Cx43 have differential effects on scrape wound closure rates in skin cells. Dose response of Gap27 in AK cells ( A ); SiRNA targeted to Cx43 and 100 nM Gap27 enhance scrape wound closure in AK ( B ) and JFF cells ( C ); Gap27 does not enhance cell migration rates in ADF or HNDF cells ( D – F ). n = 3, ** p < 0.01; *** p < 0.005.

    Article Snippet: For the purpose of this study, Gap27 (MW 1305) (Zealand Pharma, Glostrop, Denmark) was used in aqueous solution at doses ranging from 1–100 μM for 15 min–48 h depending on experimental requirements.

    Techniques: Migration

    Gap27 inhibits hemichannel signalling. JFF cells ( A ); Keratinocytes ( B ); ADF ( C ) and HNDF ( D ) cells were exposed to a dose response of Gap27 and ATP release assays performed following calcium deprivation. Data are presented at the Fold change in ATP release over control cells that were not subject to calcium challenge. n = 3, *** p < 0.005, ** p < 0.01.

    Journal: International Journal of Molecular Sciences

    Article Title: The Connexin Mimetic Peptide Gap27 and Cx43-Knockdown Reveal Differential Roles for Connexin43 in Wound Closure Events in Skin Model Systems

    doi: 10.3390/ijms19020604

    Figure Lengend Snippet: Gap27 inhibits hemichannel signalling. JFF cells ( A ); Keratinocytes ( B ); ADF ( C ) and HNDF ( D ) cells were exposed to a dose response of Gap27 and ATP release assays performed following calcium deprivation. Data are presented at the Fold change in ATP release over control cells that were not subject to calcium challenge. n = 3, *** p < 0.005, ** p < 0.01.

    Article Snippet: For the purpose of this study, Gap27 (MW 1305) (Zealand Pharma, Glostrop, Denmark) was used in aqueous solution at doses ranging from 1–100 μM for 15 min–48 h depending on experimental requirements.

    Techniques:

    The impact of Gap27 and SiRNA targeted to Cx43 on gene expression. At the end of scrape wound closure assays, RNA and protein was harvested from cells and subject to real time PCR or Western blot analysis to determine levels of Cx43 expression. JFF ( A ); AK ( B ); AF ( C ) and HNDF ( D ). Panel 1 represents changes in gene expression, Panel 2 represents a typical Western blot, Panel 3 represents densitometric analysis of three Western blots. n = 3, *** p < 0.005, ** p < 0.01 a threshold of two fold increase or decrease in expression was considered significant.

    Journal: International Journal of Molecular Sciences

    Article Title: The Connexin Mimetic Peptide Gap27 and Cx43-Knockdown Reveal Differential Roles for Connexin43 in Wound Closure Events in Skin Model Systems

    doi: 10.3390/ijms19020604

    Figure Lengend Snippet: The impact of Gap27 and SiRNA targeted to Cx43 on gene expression. At the end of scrape wound closure assays, RNA and protein was harvested from cells and subject to real time PCR or Western blot analysis to determine levels of Cx43 expression. JFF ( A ); AK ( B ); AF ( C ) and HNDF ( D ). Panel 1 represents changes in gene expression, Panel 2 represents a typical Western blot, Panel 3 represents densitometric analysis of three Western blots. n = 3, *** p < 0.005, ** p < 0.01 a threshold of two fold increase or decrease in expression was considered significant.

    Article Snippet: For the purpose of this study, Gap27 (MW 1305) (Zealand Pharma, Glostrop, Denmark) was used in aqueous solution at doses ranging from 1–100 μM for 15 min–48 h depending on experimental requirements.

    Techniques: Expressing, Real-time Polymerase Chain Reaction, Western Blot

    The impact of Gap27 and SiRNA targeted to Cx43 on Ki67 and TGF-β1 gene expression. At the end of scrape wound closure assays RNA was harvested from cells and subject to real time PCR analysis to determine changes in gene expression of ( A ) Ki67 and ( B ) TGF-β1 n = 3, *** p < 0.005, ** p < 0.01 a threshold of two fold increase or decrease in gene expression was considered significant.

    Journal: International Journal of Molecular Sciences

    Article Title: The Connexin Mimetic Peptide Gap27 and Cx43-Knockdown Reveal Differential Roles for Connexin43 in Wound Closure Events in Skin Model Systems

    doi: 10.3390/ijms19020604

    Figure Lengend Snippet: The impact of Gap27 and SiRNA targeted to Cx43 on Ki67 and TGF-β1 gene expression. At the end of scrape wound closure assays RNA was harvested from cells and subject to real time PCR analysis to determine changes in gene expression of ( A ) Ki67 and ( B ) TGF-β1 n = 3, *** p < 0.005, ** p < 0.01 a threshold of two fold increase or decrease in gene expression was considered significant.

    Article Snippet: For the purpose of this study, Gap27 (MW 1305) (Zealand Pharma, Glostrop, Denmark) was used in aqueous solution at doses ranging from 1–100 μM for 15 min–48 h depending on experimental requirements.

    Techniques: Expressing, Real-time Polymerase Chain Reaction

    The impact of Gap27 and SiRNA targeted to Cx43 on pSmad3 expression. At the end of scrape, wound closure assays protein was harvested from cells and subject to Western blot analysis. Blots were probed with an antibody targeted to pSmad3 and GAPDH. Representative blots are presented for each cell type JFF ( A ); AK ( B ); AF ( C ) and HNDF ( D ) (panel 1). Panel 2 represents densitometric analysis of triplicate blots and relative pSmad3 protein levels compared to GAPDH. n = 3, *** p < 0.005, ** p < 0.01; * p < 0.5.

    Journal: International Journal of Molecular Sciences

    Article Title: The Connexin Mimetic Peptide Gap27 and Cx43-Knockdown Reveal Differential Roles for Connexin43 in Wound Closure Events in Skin Model Systems

    doi: 10.3390/ijms19020604

    Figure Lengend Snippet: The impact of Gap27 and SiRNA targeted to Cx43 on pSmad3 expression. At the end of scrape, wound closure assays protein was harvested from cells and subject to Western blot analysis. Blots were probed with an antibody targeted to pSmad3 and GAPDH. Representative blots are presented for each cell type JFF ( A ); AK ( B ); AF ( C ) and HNDF ( D ) (panel 1). Panel 2 represents densitometric analysis of triplicate blots and relative pSmad3 protein levels compared to GAPDH. n = 3, *** p < 0.005, ** p < 0.01; * p < 0.5.

    Article Snippet: For the purpose of this study, Gap27 (MW 1305) (Zealand Pharma, Glostrop, Denmark) was used in aqueous solution at doses ranging from 1–100 μM for 15 min–48 h depending on experimental requirements.

    Techniques: Expressing, Western Blot

    Summary of the effect of 100 nM Gap27  (Gap27)  and Cx43-SiRNA (SiRNA) on cellular events related to wound closure in JFF, AK, ADF and HDNF cells. Hemichannel activity was monitored following 90 min exposure to peptide and 15 min challenge with Calcium free media. All other assays were recorded 24 h post scrape wounding in the presence or absence of 100 nM Gap27 or Cx43-SiRNA. n = 3 in all cases. ND: not determined; NE: no effect; ↑ enhanced effect; ↓ inhibitory effect. For details of experimental design and statistics see text and figures.

    Journal: International Journal of Molecular Sciences

    Article Title: The Connexin Mimetic Peptide Gap27 and Cx43-Knockdown Reveal Differential Roles for Connexin43 in Wound Closure Events in Skin Model Systems

    doi: 10.3390/ijms19020604

    Figure Lengend Snippet: Summary of the effect of 100 nM Gap27 (Gap27) and Cx43-SiRNA (SiRNA) on cellular events related to wound closure in JFF, AK, ADF and HDNF cells. Hemichannel activity was monitored following 90 min exposure to peptide and 15 min challenge with Calcium free media. All other assays were recorded 24 h post scrape wounding in the presence or absence of 100 nM Gap27 or Cx43-SiRNA. n = 3 in all cases. ND: not determined; NE: no effect; ↑ enhanced effect; ↓ inhibitory effect. For details of experimental design and statistics see text and figures.

    Article Snippet: For the purpose of this study, Gap27 (MW 1305) (Zealand Pharma, Glostrop, Denmark) was used in aqueous solution at doses ranging from 1–100 μM for 15 min–48 h depending on experimental requirements.

    Techniques: Activity Assay, Migration

    ( A ) The effect of Gap27 on PI3K signaling. U87MG cells were treated with 100 μM TMZ or 100 μM Gap27. ( B ) ATP release from Cx43-high/MGMT-deficient/TMZ-resistant SF295 cells. Cells were treated with 100 μM αCT1. Culture media were collected at different time points. ATP was measured using a colorimetric assay. Oneway ANOVA was used to determine statistical significance. ( C ) Glutamate release in Cx43-low/MGMT-deficient/TMZ-sensitive LN229 or Cx43-high/MGMT-deficient/TMZ-resistant LN229/GSC cells. Cells were treated with 100 μM TMZ and/or 100 μM αCT1. Glutamate in culture media was determined using a colorimetric assay. ( D ) ATP release in LN229 and LN229/GSC cells. ( E ) ATP within SF295 cells. ( F ) Glutamate within LN229 and LN229/GSC cells. GSC: glioblastoma stem cells.

    Journal: bioRxiv

    Article Title: Connexin 43 confers chemoresistance through activating PI3K

    doi: 10.1101/2020.10.14.339275

    Figure Lengend Snippet: ( A ) The effect of Gap27 on PI3K signaling. U87MG cells were treated with 100 μM TMZ or 100 μM Gap27. ( B ) ATP release from Cx43-high/MGMT-deficient/TMZ-resistant SF295 cells. Cells were treated with 100 μM αCT1. Culture media were collected at different time points. ATP was measured using a colorimetric assay. Oneway ANOVA was used to determine statistical significance. ( C ) Glutamate release in Cx43-low/MGMT-deficient/TMZ-sensitive LN229 or Cx43-high/MGMT-deficient/TMZ-resistant LN229/GSC cells. Cells were treated with 100 μM TMZ and/or 100 μM αCT1. Glutamate in culture media was determined using a colorimetric assay. ( D ) ATP release in LN229 and LN229/GSC cells. ( E ) ATP within SF295 cells. ( F ) Glutamate within LN229 and LN229/GSC cells. GSC: glioblastoma stem cells.

    Article Snippet: TMZ (AbMole BioScience), GSK2636771 (AdooQ Bioscience), TGX-221 (AdooQ Bioscience) were reconstituted in dimethyl sulfoxide (DMSO) at a concentration of 50-80 mM. αCT1 and Gap27 were purchased from LifeTein, LLC.

    Techniques: Colorimetric Assay