Journal: eLife

Article Title: Structure of Mycobacterium tuberculosis cytochrome bcc in complex with Q203 and TB47, two anti-TB drug candidates

doi: 10.7554/eLife.69418

Figure Lengend Snippet: ( A ) Representative electron micrograph of the cryo-EM sample. ( B ) CTF fit of the motion-corrected micrographs. ( C ) Representative 2D classification averages calculated from selected particles. ( D ) Workflow of data processing for the apo hybrid supercomplex. ( E ) Fourier shell correlation (FSC) curves of 3D reconstructions. ( F ) View direction of all particles used in the final 3D reconstruction. ( G ) 3D FSC histogram of the final map. ( H ) The overall and Mtb cytochrome bcc maps, colored according to the local resolution.

Article Snippet: The following dataset was generated: Zhou S Wang W Gao Y Gong H Rao Z 2021 Cryo-EM structure of apo hybrid respiratory supercomplex consisting of Mycobacterium tuberculosis complexIII and Mycobacterium smegmatis complexIV Electron Microscopy Data Bank EMD-30943 Zhou S Wang W Gao Y Gong H Rao Z 2021 Cryo-EM structure of hybrid respiratory supercomplex consisting of Mycobacterium tuberculosis complexIII and Mycobacterium smegmatis complexIV in the presence of Q203 Electron Microscopy Data Bank EMD-30944 Zhou S Wang W Gao Y Gong H Rao Z 2021 Cryo-EM structure of hybrid respiratory supercomplex consisting of Mycobacterium tuberculosis complexIII and Mycobacterium smegmatis complexIV in presence of TB47 Electron Microscopy Data Bank EMD-30945 Zhou S Wang W Gao Y Gong H Rao Z 2021 Cryo-EM structure of apo hybrid respiratory supercomplex consisting of Mycobacterium tuberculosis complexIII and Mycobacterium smegmatis complexIV RCSB Protein Data Bank 7E1V Zhou S Wang W Gao Y Gong H Rao Z 2021 Cryo-EM structure of hybrid respiratory supercomplex consisting of Mycobacterium tuberculosis complexIII and Mycobacterium smegmatis complexIV in the presence of Q203 RCSB Protein Data Bank 7E1W Zhou S Wang W Gao Y Gong H Rao Z 2021 Cryo-EM structure of hybrid respiratory supercomplex consisting of Mycobacterium tuberculosis complexIII and Mycobacterium smegmatis complexIV in presence of TB47 RCSB Protein Data Bank 7E1X

Techniques: Cryo-EM Sample Prep

Journal: eLife

Article Title: Structure of Mycobacterium tuberculosis cytochrome bcc in complex with Q203 and TB47, two anti-TB drug candidates

doi: 10.7554/eLife.69418

Figure Lengend Snippet: Representative cryo-EM densities of regions within individual subunits and prosthetic groups. Corresponding subunits with residues and prosthetic groups shown in cartoon representation or as stick models. Map-model Fourier shell correlation (FSC).

Article Snippet: The following dataset was generated: Zhou S Wang W Gao Y Gong H Rao Z 2021 Cryo-EM structure of apo hybrid respiratory supercomplex consisting of Mycobacterium tuberculosis complexIII and Mycobacterium smegmatis complexIV Electron Microscopy Data Bank EMD-30943 Zhou S Wang W Gao Y Gong H Rao Z 2021 Cryo-EM structure of hybrid respiratory supercomplex consisting of Mycobacterium tuberculosis complexIII and Mycobacterium smegmatis complexIV in the presence of Q203 Electron Microscopy Data Bank EMD-30944 Zhou S Wang W Gao Y Gong H Rao Z 2021 Cryo-EM structure of hybrid respiratory supercomplex consisting of Mycobacterium tuberculosis complexIII and Mycobacterium smegmatis complexIV in presence of TB47 Electron Microscopy Data Bank EMD-30945 Zhou S Wang W Gao Y Gong H Rao Z 2021 Cryo-EM structure of apo hybrid respiratory supercomplex consisting of Mycobacterium tuberculosis complexIII and Mycobacterium smegmatis complexIV RCSB Protein Data Bank 7E1V Zhou S Wang W Gao Y Gong H Rao Z 2021 Cryo-EM structure of hybrid respiratory supercomplex consisting of Mycobacterium tuberculosis complexIII and Mycobacterium smegmatis complexIV in the presence of Q203 RCSB Protein Data Bank 7E1W Zhou S Wang W Gao Y Gong H Rao Z 2021 Cryo-EM structure of hybrid respiratory supercomplex consisting of Mycobacterium tuberculosis complexIII and Mycobacterium smegmatis complexIV in presence of TB47 RCSB Protein Data Bank 7E1X

Techniques: Cryo-EM Sample Prep

Journal: eLife

Article Title: Structure of Mycobacterium tuberculosis cytochrome bcc in complex with Q203 and TB47, two anti-TB drug candidates

doi: 10.7554/eLife.69418

Figure Lengend Snippet: ( A ) Front view and ( B ) top view of the cryo-electron microscopy (cryo-EM) map of hybrid supercomplex at 2.68 Å resolution. QcrA, QcrB, and QcrC of the M. tuberculosis cytochrome bcc are colored teal, purple, and salmon, respectively. Other subunits of the hybrid supercomplex are in gray. ( C ) Cartoon representation of cytochrome bcc , using the same color scheme as above. The twofold symmetry of the dimer is depicted by the gray axis. The heme groups ( b H , b L , c D1 , and c D2 ) and menaquinone/menaquinol (MK P /MK N ) are shown as stick models. The [2Fe-2S] clusters are shown as spheres. ( D ) A cross-sectional view (top) of the helices in the cytochrome bcc dimer. Figure 2—source data 1. Oxygen consumption of the hybrid supercomplex measures using Clark-type oxygen electrode.

Article Snippet: The following dataset was generated: Zhou S Wang W Gao Y Gong H Rao Z 2021 Cryo-EM structure of apo hybrid respiratory supercomplex consisting of Mycobacterium tuberculosis complexIII and Mycobacterium smegmatis complexIV Electron Microscopy Data Bank EMD-30943 Zhou S Wang W Gao Y Gong H Rao Z 2021 Cryo-EM structure of hybrid respiratory supercomplex consisting of Mycobacterium tuberculosis complexIII and Mycobacterium smegmatis complexIV in the presence of Q203 Electron Microscopy Data Bank EMD-30944 Zhou S Wang W Gao Y Gong H Rao Z 2021 Cryo-EM structure of hybrid respiratory supercomplex consisting of Mycobacterium tuberculosis complexIII and Mycobacterium smegmatis complexIV in presence of TB47 Electron Microscopy Data Bank EMD-30945 Zhou S Wang W Gao Y Gong H Rao Z 2021 Cryo-EM structure of apo hybrid respiratory supercomplex consisting of Mycobacterium tuberculosis complexIII and Mycobacterium smegmatis complexIV RCSB Protein Data Bank 7E1V Zhou S Wang W Gao Y Gong H Rao Z 2021 Cryo-EM structure of hybrid respiratory supercomplex consisting of Mycobacterium tuberculosis complexIII and Mycobacterium smegmatis complexIV in the presence of Q203 RCSB Protein Data Bank 7E1W Zhou S Wang W Gao Y Gong H Rao Z 2021 Cryo-EM structure of hybrid respiratory supercomplex consisting of Mycobacterium tuberculosis complexIII and Mycobacterium smegmatis complexIV in presence of TB47 RCSB Protein Data Bank 7E1X

Techniques: Electron Microscopy, Cryo-EM Sample Prep

Journal: eLife

Article Title: Structure of Mycobacterium tuberculosis cytochrome bcc in complex with Q203 and TB47, two anti-TB drug candidates

doi: 10.7554/eLife.69418

Figure Lengend Snippet: The QcrB is extracted from the hybrid supercomplex with Q203 (purple) and MK9 (gray) bound is shown in cartoon representation.

Article Snippet: The following dataset was generated: Zhou S Wang W Gao Y Gong H Rao Z 2021 Cryo-EM structure of apo hybrid respiratory supercomplex consisting of Mycobacterium tuberculosis complexIII and Mycobacterium smegmatis complexIV Electron Microscopy Data Bank EMD-30943 Zhou S Wang W Gao Y Gong H Rao Z 2021 Cryo-EM structure of hybrid respiratory supercomplex consisting of Mycobacterium tuberculosis complexIII and Mycobacterium smegmatis complexIV in the presence of Q203 Electron Microscopy Data Bank EMD-30944 Zhou S Wang W Gao Y Gong H Rao Z 2021 Cryo-EM structure of hybrid respiratory supercomplex consisting of Mycobacterium tuberculosis complexIII and Mycobacterium smegmatis complexIV in presence of TB47 Electron Microscopy Data Bank EMD-30945 Zhou S Wang W Gao Y Gong H Rao Z 2021 Cryo-EM structure of apo hybrid respiratory supercomplex consisting of Mycobacterium tuberculosis complexIII and Mycobacterium smegmatis complexIV RCSB Protein Data Bank 7E1V Zhou S Wang W Gao Y Gong H Rao Z 2021 Cryo-EM structure of hybrid respiratory supercomplex consisting of Mycobacterium tuberculosis complexIII and Mycobacterium smegmatis complexIV in the presence of Q203 RCSB Protein Data Bank 7E1W Zhou S Wang W Gao Y Gong H Rao Z 2021 Cryo-EM structure of hybrid respiratory supercomplex consisting of Mycobacterium tuberculosis complexIII and Mycobacterium smegmatis complexIV in presence of TB47 RCSB Protein Data Bank 7E1X

Techniques:

Journal: eLife

Article Title: Structure of Mycobacterium tuberculosis cytochrome bcc in complex with Q203 and TB47, two anti-TB drug candidates

doi: 10.7554/eLife.69418

Figure Lengend Snippet: ( A ) Representative electron micrograph of the cryo-EM sample. ( B ) CTF fit of motion-corrected micrographs ( C ) Representative 2D classification averages calculated from selected particles. ( D ) Workflow of data processing for the Q203-bound hybrid supercomplex. ( E ) Fourier shell correlation (FSC) curves of 3D reconstructions. ( F ) Viewing direction of all particles used in the final 3D reconstruction. ( G ) 3D FSC histogram of final map. ( H ) The overall and Mtb cytochrome bcc maps, colored according to the local resolution.

Article Snippet: The following dataset was generated: Zhou S Wang W Gao Y Gong H Rao Z 2021 Cryo-EM structure of apo hybrid respiratory supercomplex consisting of Mycobacterium tuberculosis complexIII and Mycobacterium smegmatis complexIV Electron Microscopy Data Bank EMD-30943 Zhou S Wang W Gao Y Gong H Rao Z 2021 Cryo-EM structure of hybrid respiratory supercomplex consisting of Mycobacterium tuberculosis complexIII and Mycobacterium smegmatis complexIV in the presence of Q203 Electron Microscopy Data Bank EMD-30944 Zhou S Wang W Gao Y Gong H Rao Z 2021 Cryo-EM structure of hybrid respiratory supercomplex consisting of Mycobacterium tuberculosis complexIII and Mycobacterium smegmatis complexIV in presence of TB47 Electron Microscopy Data Bank EMD-30945 Zhou S Wang W Gao Y Gong H Rao Z 2021 Cryo-EM structure of apo hybrid respiratory supercomplex consisting of Mycobacterium tuberculosis complexIII and Mycobacterium smegmatis complexIV RCSB Protein Data Bank 7E1V Zhou S Wang W Gao Y Gong H Rao Z 2021 Cryo-EM structure of hybrid respiratory supercomplex consisting of Mycobacterium tuberculosis complexIII and Mycobacterium smegmatis complexIV in the presence of Q203 RCSB Protein Data Bank 7E1W Zhou S Wang W Gao Y Gong H Rao Z 2021 Cryo-EM structure of hybrid respiratory supercomplex consisting of Mycobacterium tuberculosis complexIII and Mycobacterium smegmatis complexIV in presence of TB47 RCSB Protein Data Bank 7E1X

Techniques: Cryo-EM Sample Prep

Journal: eLife

Article Title: Structure of Mycobacterium tuberculosis cytochrome bcc in complex with Q203 and TB47, two anti-TB drug candidates

doi: 10.7554/eLife.69418

Figure Lengend Snippet: Representative cryo-EM densities and structures of individual subunits, prosthetic groups, and inhibitors. Map-model Fourier shell correlation (FSC).

Article Snippet: The following dataset was generated: Zhou S Wang W Gao Y Gong H Rao Z 2021 Cryo-EM structure of apo hybrid respiratory supercomplex consisting of Mycobacterium tuberculosis complexIII and Mycobacterium smegmatis complexIV Electron Microscopy Data Bank EMD-30943 Zhou S Wang W Gao Y Gong H Rao Z 2021 Cryo-EM structure of hybrid respiratory supercomplex consisting of Mycobacterium tuberculosis complexIII and Mycobacterium smegmatis complexIV in the presence of Q203 Electron Microscopy Data Bank EMD-30944 Zhou S Wang W Gao Y Gong H Rao Z 2021 Cryo-EM structure of hybrid respiratory supercomplex consisting of Mycobacterium tuberculosis complexIII and Mycobacterium smegmatis complexIV in presence of TB47 Electron Microscopy Data Bank EMD-30945 Zhou S Wang W Gao Y Gong H Rao Z 2021 Cryo-EM structure of apo hybrid respiratory supercomplex consisting of Mycobacterium tuberculosis complexIII and Mycobacterium smegmatis complexIV RCSB Protein Data Bank 7E1V Zhou S Wang W Gao Y Gong H Rao Z 2021 Cryo-EM structure of hybrid respiratory supercomplex consisting of Mycobacterium tuberculosis complexIII and Mycobacterium smegmatis complexIV in the presence of Q203 RCSB Protein Data Bank 7E1W Zhou S Wang W Gao Y Gong H Rao Z 2021 Cryo-EM structure of hybrid respiratory supercomplex consisting of Mycobacterium tuberculosis complexIII and Mycobacterium smegmatis complexIV in presence of TB47 RCSB Protein Data Bank 7E1X

Techniques: Cryo-EM Sample Prep

Journal: eLife

Article Title: Structure of Mycobacterium tuberculosis cytochrome bcc in complex with Q203 and TB47, two anti-TB drug candidates

doi: 10.7554/eLife.69418

Figure Lengend Snippet: Cryo-electron microscopy data collection, refinement, and validation statistics for the Q203-bound hybrid supercomplex.

Article Snippet: The following dataset was generated: Zhou S Wang W Gao Y Gong H Rao Z 2021 Cryo-EM structure of apo hybrid respiratory supercomplex consisting of Mycobacterium tuberculosis complexIII and Mycobacterium smegmatis complexIV Electron Microscopy Data Bank EMD-30943 Zhou S Wang W Gao Y Gong H Rao Z 2021 Cryo-EM structure of hybrid respiratory supercomplex consisting of Mycobacterium tuberculosis complexIII and Mycobacterium smegmatis complexIV in the presence of Q203 Electron Microscopy Data Bank EMD-30944 Zhou S Wang W Gao Y Gong H Rao Z 2021 Cryo-EM structure of hybrid respiratory supercomplex consisting of Mycobacterium tuberculosis complexIII and Mycobacterium smegmatis complexIV in presence of TB47 Electron Microscopy Data Bank EMD-30945 Zhou S Wang W Gao Y Gong H Rao Z 2021 Cryo-EM structure of apo hybrid respiratory supercomplex consisting of Mycobacterium tuberculosis complexIII and Mycobacterium smegmatis complexIV RCSB Protein Data Bank 7E1V Zhou S Wang W Gao Y Gong H Rao Z 2021 Cryo-EM structure of hybrid respiratory supercomplex consisting of Mycobacterium tuberculosis complexIII and Mycobacterium smegmatis complexIV in the presence of Q203 RCSB Protein Data Bank 7E1W Zhou S Wang W Gao Y Gong H Rao Z 2021 Cryo-EM structure of hybrid respiratory supercomplex consisting of Mycobacterium tuberculosis complexIII and Mycobacterium smegmatis complexIV in presence of TB47 RCSB Protein Data Bank 7E1X

Techniques: Microscopy, Software

Journal: eLife

Article Title: Structure of Mycobacterium tuberculosis cytochrome bcc in complex with Q203 and TB47, two anti-TB drug candidates

doi: 10.7554/eLife.69418

Figure Lengend Snippet: ( A ) Side (left) and top (right) views of the cryo-EM structure of the M. tuberculosis cytochrome bcc complex presented as a cartoon representation. Q203 (orange) is bound to the Qp site. ( B ) Visualization of densities for Q203. Hydrogen bonds are shown as dotted lines. ( C ) Plot of distances of various parts of Q203 to residues in the Qp site as determined using LIGPLOT ( https://www.ebi.ac.uk/thornton-srv/software/LIGPLOT/ ).

Article Snippet: The following dataset was generated: Zhou S Wang W Gao Y Gong H Rao Z 2021 Cryo-EM structure of apo hybrid respiratory supercomplex consisting of Mycobacterium tuberculosis complexIII and Mycobacterium smegmatis complexIV Electron Microscopy Data Bank EMD-30943 Zhou S Wang W Gao Y Gong H Rao Z 2021 Cryo-EM structure of hybrid respiratory supercomplex consisting of Mycobacterium tuberculosis complexIII and Mycobacterium smegmatis complexIV in the presence of Q203 Electron Microscopy Data Bank EMD-30944 Zhou S Wang W Gao Y Gong H Rao Z 2021 Cryo-EM structure of hybrid respiratory supercomplex consisting of Mycobacterium tuberculosis complexIII and Mycobacterium smegmatis complexIV in presence of TB47 Electron Microscopy Data Bank EMD-30945 Zhou S Wang W Gao Y Gong H Rao Z 2021 Cryo-EM structure of apo hybrid respiratory supercomplex consisting of Mycobacterium tuberculosis complexIII and Mycobacterium smegmatis complexIV RCSB Protein Data Bank 7E1V Zhou S Wang W Gao Y Gong H Rao Z 2021 Cryo-EM structure of hybrid respiratory supercomplex consisting of Mycobacterium tuberculosis complexIII and Mycobacterium smegmatis complexIV in the presence of Q203 RCSB Protein Data Bank 7E1W Zhou S Wang W Gao Y Gong H Rao Z 2021 Cryo-EM structure of hybrid respiratory supercomplex consisting of Mycobacterium tuberculosis complexIII and Mycobacterium smegmatis complexIV in presence of TB47 RCSB Protein Data Bank 7E1X

Techniques: Cryo-EM Sample Prep, Software

Journal: eLife

Article Title: Structure of Mycobacterium tuberculosis cytochrome bcc in complex with Q203 and TB47, two anti-TB drug candidates

doi: 10.7554/eLife.69418

Figure Lengend Snippet: Continuous density is observed between the head of Q203, a likely bridging water molecule, and the side chain of QcrB Tyr 164 .

Article Snippet: The following dataset was generated: Zhou S Wang W Gao Y Gong H Rao Z 2021 Cryo-EM structure of apo hybrid respiratory supercomplex consisting of Mycobacterium tuberculosis complexIII and Mycobacterium smegmatis complexIV Electron Microscopy Data Bank EMD-30943 Zhou S Wang W Gao Y Gong H Rao Z 2021 Cryo-EM structure of hybrid respiratory supercomplex consisting of Mycobacterium tuberculosis complexIII and Mycobacterium smegmatis complexIV in the presence of Q203 Electron Microscopy Data Bank EMD-30944 Zhou S Wang W Gao Y Gong H Rao Z 2021 Cryo-EM structure of hybrid respiratory supercomplex consisting of Mycobacterium tuberculosis complexIII and Mycobacterium smegmatis complexIV in presence of TB47 Electron Microscopy Data Bank EMD-30945 Zhou S Wang W Gao Y Gong H Rao Z 2021 Cryo-EM structure of apo hybrid respiratory supercomplex consisting of Mycobacterium tuberculosis complexIII and Mycobacterium smegmatis complexIV RCSB Protein Data Bank 7E1V Zhou S Wang W Gao Y Gong H Rao Z 2021 Cryo-EM structure of hybrid respiratory supercomplex consisting of Mycobacterium tuberculosis complexIII and Mycobacterium smegmatis complexIV in the presence of Q203 RCSB Protein Data Bank 7E1W Zhou S Wang W Gao Y Gong H Rao Z 2021 Cryo-EM structure of hybrid respiratory supercomplex consisting of Mycobacterium tuberculosis complexIII and Mycobacterium smegmatis complexIV in presence of TB47 RCSB Protein Data Bank 7E1X

Techniques:

Journal: eLife

Article Title: Structure of Mycobacterium tuberculosis cytochrome bcc in complex with Q203 and TB47, two anti-TB drug candidates

doi: 10.7554/eLife.69418

Figure Lengend Snippet: ( A ) Side (left) and top (right) views of the cryo-EM structure of the M. tuberculosis cytochrome bcc complex presented as a cartoon representation. TB47 (green) and Q203 (orange) are bound to the Qp site. ( B ) Visualization of the density for TB47. Hydrogen bonds are shown as dotted lines. ( C ) Plot of distances of various parts of TB47 to residues in the Qp site were determined using LIGPLOT ( https://www.ebi.ac.uk/thornton-srv/software/LIGPLOT/ ).

Article Snippet: The following dataset was generated: Zhou S Wang W Gao Y Gong H Rao Z 2021 Cryo-EM structure of apo hybrid respiratory supercomplex consisting of Mycobacterium tuberculosis complexIII and Mycobacterium smegmatis complexIV Electron Microscopy Data Bank EMD-30943 Zhou S Wang W Gao Y Gong H Rao Z 2021 Cryo-EM structure of hybrid respiratory supercomplex consisting of Mycobacterium tuberculosis complexIII and Mycobacterium smegmatis complexIV in the presence of Q203 Electron Microscopy Data Bank EMD-30944 Zhou S Wang W Gao Y Gong H Rao Z 2021 Cryo-EM structure of hybrid respiratory supercomplex consisting of Mycobacterium tuberculosis complexIII and Mycobacterium smegmatis complexIV in presence of TB47 Electron Microscopy Data Bank EMD-30945 Zhou S Wang W Gao Y Gong H Rao Z 2021 Cryo-EM structure of apo hybrid respiratory supercomplex consisting of Mycobacterium tuberculosis complexIII and Mycobacterium smegmatis complexIV RCSB Protein Data Bank 7E1V Zhou S Wang W Gao Y Gong H Rao Z 2021 Cryo-EM structure of hybrid respiratory supercomplex consisting of Mycobacterium tuberculosis complexIII and Mycobacterium smegmatis complexIV in the presence of Q203 RCSB Protein Data Bank 7E1W Zhou S Wang W Gao Y Gong H Rao Z 2021 Cryo-EM structure of hybrid respiratory supercomplex consisting of Mycobacterium tuberculosis complexIII and Mycobacterium smegmatis complexIV in presence of TB47 RCSB Protein Data Bank 7E1X

Techniques: Cryo-EM Sample Prep, Software

Journal: eLife

Article Title: Structure of Mycobacterium tuberculosis cytochrome bcc in complex with Q203 and TB47, two anti-TB drug candidates

doi: 10.7554/eLife.69418

Figure Lengend Snippet: ( A ) Representative electron micrograph of the cryo-EM sample. ( B ) CTF fit of motion-corrected micrographs ( C ) Representative 2D classification averages calculated from selected particles. ( D ) Workflow of the data processing for the TB47-bound hybrid supercomplex. ( E ) Fourier shell correlation (FSC) curves of 3D reconstructions. ( F ) Viewing direction of all particles used in the final 3D reconstruction. ( G ) 3D FSC histogram of final map. ( H ) The overall and M. tuberculosis cytochrome bcc maps, colored according to the local resolution.

Article Snippet: The following dataset was generated: Zhou S Wang W Gao Y Gong H Rao Z 2021 Cryo-EM structure of apo hybrid respiratory supercomplex consisting of Mycobacterium tuberculosis complexIII and Mycobacterium smegmatis complexIV Electron Microscopy Data Bank EMD-30943 Zhou S Wang W Gao Y Gong H Rao Z 2021 Cryo-EM structure of hybrid respiratory supercomplex consisting of Mycobacterium tuberculosis complexIII and Mycobacterium smegmatis complexIV in the presence of Q203 Electron Microscopy Data Bank EMD-30944 Zhou S Wang W Gao Y Gong H Rao Z 2021 Cryo-EM structure of hybrid respiratory supercomplex consisting of Mycobacterium tuberculosis complexIII and Mycobacterium smegmatis complexIV in presence of TB47 Electron Microscopy Data Bank EMD-30945 Zhou S Wang W Gao Y Gong H Rao Z 2021 Cryo-EM structure of apo hybrid respiratory supercomplex consisting of Mycobacterium tuberculosis complexIII and Mycobacterium smegmatis complexIV RCSB Protein Data Bank 7E1V Zhou S Wang W Gao Y Gong H Rao Z 2021 Cryo-EM structure of hybrid respiratory supercomplex consisting of Mycobacterium tuberculosis complexIII and Mycobacterium smegmatis complexIV in the presence of Q203 RCSB Protein Data Bank 7E1W Zhou S Wang W Gao Y Gong H Rao Z 2021 Cryo-EM structure of hybrid respiratory supercomplex consisting of Mycobacterium tuberculosis complexIII and Mycobacterium smegmatis complexIV in presence of TB47 RCSB Protein Data Bank 7E1X

Techniques: Cryo-EM Sample Prep

Journal: eLife

Article Title: Structure of Mycobacterium tuberculosis cytochrome bcc in complex with Q203 and TB47, two anti-TB drug candidates

doi: 10.7554/eLife.69418

Figure Lengend Snippet: Representative cryo-EM densities and structures of individual subunits, prosthetic groups, and inhibitors. Map-model Fourier shell correlation (FSC).

Article Snippet: The following dataset was generated: Zhou S Wang W Gao Y Gong H Rao Z 2021 Cryo-EM structure of apo hybrid respiratory supercomplex consisting of Mycobacterium tuberculosis complexIII and Mycobacterium smegmatis complexIV Electron Microscopy Data Bank EMD-30943 Zhou S Wang W Gao Y Gong H Rao Z 2021 Cryo-EM structure of hybrid respiratory supercomplex consisting of Mycobacterium tuberculosis complexIII and Mycobacterium smegmatis complexIV in the presence of Q203 Electron Microscopy Data Bank EMD-30944 Zhou S Wang W Gao Y Gong H Rao Z 2021 Cryo-EM structure of hybrid respiratory supercomplex consisting of Mycobacterium tuberculosis complexIII and Mycobacterium smegmatis complexIV in presence of TB47 Electron Microscopy Data Bank EMD-30945 Zhou S Wang W Gao Y Gong H Rao Z 2021 Cryo-EM structure of apo hybrid respiratory supercomplex consisting of Mycobacterium tuberculosis complexIII and Mycobacterium smegmatis complexIV RCSB Protein Data Bank 7E1V Zhou S Wang W Gao Y Gong H Rao Z 2021 Cryo-EM structure of hybrid respiratory supercomplex consisting of Mycobacterium tuberculosis complexIII and Mycobacterium smegmatis complexIV in the presence of Q203 RCSB Protein Data Bank 7E1W Zhou S Wang W Gao Y Gong H Rao Z 2021 Cryo-EM structure of hybrid respiratory supercomplex consisting of Mycobacterium tuberculosis complexIII and Mycobacterium smegmatis complexIV in presence of TB47 RCSB Protein Data Bank 7E1X

Techniques: Cryo-EM Sample Prep

Journal: eLife

Article Title: Structure of Mycobacterium tuberculosis cytochrome bcc in complex with Q203 and TB47, two anti-TB drug candidates

doi: 10.7554/eLife.69418

Figure Lengend Snippet: Relative binding free energy (kcal/mol) for Q203 in three mutants of QcrB compared to the wild-type (WT).

Article Snippet: The following dataset was generated: Zhou S Wang W Gao Y Gong H Rao Z 2021 Cryo-EM structure of apo hybrid respiratory supercomplex consisting of Mycobacterium tuberculosis complexIII and Mycobacterium smegmatis complexIV Electron Microscopy Data Bank EMD-30943 Zhou S Wang W Gao Y Gong H Rao Z 2021 Cryo-EM structure of hybrid respiratory supercomplex consisting of Mycobacterium tuberculosis complexIII and Mycobacterium smegmatis complexIV in the presence of Q203 Electron Microscopy Data Bank EMD-30944 Zhou S Wang W Gao Y Gong H Rao Z 2021 Cryo-EM structure of hybrid respiratory supercomplex consisting of Mycobacterium tuberculosis complexIII and Mycobacterium smegmatis complexIV in presence of TB47 Electron Microscopy Data Bank EMD-30945 Zhou S Wang W Gao Y Gong H Rao Z 2021 Cryo-EM structure of apo hybrid respiratory supercomplex consisting of Mycobacterium tuberculosis complexIII and Mycobacterium smegmatis complexIV RCSB Protein Data Bank 7E1V Zhou S Wang W Gao Y Gong H Rao Z 2021 Cryo-EM structure of hybrid respiratory supercomplex consisting of Mycobacterium tuberculosis complexIII and Mycobacterium smegmatis complexIV in the presence of Q203 RCSB Protein Data Bank 7E1W Zhou S Wang W Gao Y Gong H Rao Z 2021 Cryo-EM structure of hybrid respiratory supercomplex consisting of Mycobacterium tuberculosis complexIII and Mycobacterium smegmatis complexIV in presence of TB47 RCSB Protein Data Bank 7E1X

Techniques: Binding Assay, Standard Deviation

Journal: eLife

Article Title: Structure of Mycobacterium tuberculosis cytochrome bcc in complex with Q203 and TB47, two anti-TB drug candidates

doi: 10.7554/eLife.69418

Figure Lengend Snippet: ( A ) Superposition of apo (gray), Q203-bound (cyan), and TB47-bound (magenta) structures of M. tuberculosis cytochromes bcc . The Q203 (orange), TB47 (green), and MK (yellow) molecules are shown as stick models, respectively. Water molecules are shown as spheres. ( B ) Comparison of residues surrounding Q203 (cyan sticks) and TB47 (magenta stick models) with those in apo form (gray sticks). The residues from subunits A and B are labeled with superscript A and B, respectively.

Article Snippet: The following dataset was generated: Zhou S Wang W Gao Y Gong H Rao Z 2021 Cryo-EM structure of apo hybrid respiratory supercomplex consisting of Mycobacterium tuberculosis complexIII and Mycobacterium smegmatis complexIV Electron Microscopy Data Bank EMD-30943 Zhou S Wang W Gao Y Gong H Rao Z 2021 Cryo-EM structure of hybrid respiratory supercomplex consisting of Mycobacterium tuberculosis complexIII and Mycobacterium smegmatis complexIV in the presence of Q203 Electron Microscopy Data Bank EMD-30944 Zhou S Wang W Gao Y Gong H Rao Z 2021 Cryo-EM structure of hybrid respiratory supercomplex consisting of Mycobacterium tuberculosis complexIII and Mycobacterium smegmatis complexIV in presence of TB47 Electron Microscopy Data Bank EMD-30945 Zhou S Wang W Gao Y Gong H Rao Z 2021 Cryo-EM structure of apo hybrid respiratory supercomplex consisting of Mycobacterium tuberculosis complexIII and Mycobacterium smegmatis complexIV RCSB Protein Data Bank 7E1V Zhou S Wang W Gao Y Gong H Rao Z 2021 Cryo-EM structure of hybrid respiratory supercomplex consisting of Mycobacterium tuberculosis complexIII and Mycobacterium smegmatis complexIV in the presence of Q203 RCSB Protein Data Bank 7E1W Zhou S Wang W Gao Y Gong H Rao Z 2021 Cryo-EM structure of hybrid respiratory supercomplex consisting of Mycobacterium tuberculosis complexIII and Mycobacterium smegmatis complexIV in presence of TB47 RCSB Protein Data Bank 7E1X

Techniques: Labeling

Journal: eLife

Article Title: Structure of Mycobacterium tuberculosis cytochrome bcc in complex with Q203 and TB47, two anti-TB drug candidates

doi: 10.7554/eLife.69418

Figure Lengend Snippet: The two monomers of M. tuberculosis cytochrome bcc are colored magenta and cyan, respectively. The binding of Q203 (orange spheres) or TB47 (green spheres) prevents substrate access (gray spheres).

Article Snippet: The following dataset was generated: Zhou S Wang W Gao Y Gong H Rao Z 2021 Cryo-EM structure of apo hybrid respiratory supercomplex consisting of Mycobacterium tuberculosis complexIII and Mycobacterium smegmatis complexIV Electron Microscopy Data Bank EMD-30943 Zhou S Wang W Gao Y Gong H Rao Z 2021 Cryo-EM structure of hybrid respiratory supercomplex consisting of Mycobacterium tuberculosis complexIII and Mycobacterium smegmatis complexIV in the presence of Q203 Electron Microscopy Data Bank EMD-30944 Zhou S Wang W Gao Y Gong H Rao Z 2021 Cryo-EM structure of hybrid respiratory supercomplex consisting of Mycobacterium tuberculosis complexIII and Mycobacterium smegmatis complexIV in presence of TB47 Electron Microscopy Data Bank EMD-30945 Zhou S Wang W Gao Y Gong H Rao Z 2021 Cryo-EM structure of apo hybrid respiratory supercomplex consisting of Mycobacterium tuberculosis complexIII and Mycobacterium smegmatis complexIV RCSB Protein Data Bank 7E1V Zhou S Wang W Gao Y Gong H Rao Z 2021 Cryo-EM structure of hybrid respiratory supercomplex consisting of Mycobacterium tuberculosis complexIII and Mycobacterium smegmatis complexIV in the presence of Q203 RCSB Protein Data Bank 7E1W Zhou S Wang W Gao Y Gong H Rao Z 2021 Cryo-EM structure of hybrid respiratory supercomplex consisting of Mycobacterium tuberculosis complexIII and Mycobacterium smegmatis complexIV in presence of TB47 RCSB Protein Data Bank 7E1X

Techniques: Binding Assay

Journal: eLife

Article Title: Structure of Mycobacterium tuberculosis cytochrome bcc in complex with Q203 and TB47, two anti-TB drug candidates

doi: 10.7554/eLife.69418

Figure Lengend Snippet: Partial sequence alignment at the Qp site. The red triangles indicate the conservative residues involved with the hydrogen-bond formation between Q203 and TB47.

Article Snippet: The following dataset was generated: Zhou S Wang W Gao Y Gong H Rao Z 2021 Cryo-EM structure of apo hybrid respiratory supercomplex consisting of Mycobacterium tuberculosis complexIII and Mycobacterium smegmatis complexIV Electron Microscopy Data Bank EMD-30943 Zhou S Wang W Gao Y Gong H Rao Z 2021 Cryo-EM structure of hybrid respiratory supercomplex consisting of Mycobacterium tuberculosis complexIII and Mycobacterium smegmatis complexIV in the presence of Q203 Electron Microscopy Data Bank EMD-30944 Zhou S Wang W Gao Y Gong H Rao Z 2021 Cryo-EM structure of hybrid respiratory supercomplex consisting of Mycobacterium tuberculosis complexIII and Mycobacterium smegmatis complexIV in presence of TB47 Electron Microscopy Data Bank EMD-30945 Zhou S Wang W Gao Y Gong H Rao Z 2021 Cryo-EM structure of apo hybrid respiratory supercomplex consisting of Mycobacterium tuberculosis complexIII and Mycobacterium smegmatis complexIV RCSB Protein Data Bank 7E1V Zhou S Wang W Gao Y Gong H Rao Z 2021 Cryo-EM structure of hybrid respiratory supercomplex consisting of Mycobacterium tuberculosis complexIII and Mycobacterium smegmatis complexIV in the presence of Q203 RCSB Protein Data Bank 7E1W Zhou S Wang W Gao Y Gong H Rao Z 2021 Cryo-EM structure of hybrid respiratory supercomplex consisting of Mycobacterium tuberculosis complexIII and Mycobacterium smegmatis complexIV in presence of TB47 RCSB Protein Data Bank 7E1X

Techniques: Sequencing

Journal: eLife

Article Title: Structure of Mycobacterium tuberculosis cytochrome bcc in complex with Q203 and TB47, two anti-TB drug candidates

doi: 10.7554/eLife.69418

Figure Lengend Snippet:

Article Snippet: The following dataset was generated: Zhou S Wang W Gao Y Gong H Rao Z 2021 Cryo-EM structure of apo hybrid respiratory supercomplex consisting of Mycobacterium tuberculosis complexIII and Mycobacterium smegmatis complexIV Electron Microscopy Data Bank EMD-30943 Zhou S Wang W Gao Y Gong H Rao Z 2021 Cryo-EM structure of hybrid respiratory supercomplex consisting of Mycobacterium tuberculosis complexIII and Mycobacterium smegmatis complexIV in the presence of Q203 Electron Microscopy Data Bank EMD-30944 Zhou S Wang W Gao Y Gong H Rao Z 2021 Cryo-EM structure of hybrid respiratory supercomplex consisting of Mycobacterium tuberculosis complexIII and Mycobacterium smegmatis complexIV in presence of TB47 Electron Microscopy Data Bank EMD-30945 Zhou S Wang W Gao Y Gong H Rao Z 2021 Cryo-EM structure of apo hybrid respiratory supercomplex consisting of Mycobacterium tuberculosis complexIII and Mycobacterium smegmatis complexIV RCSB Protein Data Bank 7E1V Zhou S Wang W Gao Y Gong H Rao Z 2021 Cryo-EM structure of hybrid respiratory supercomplex consisting of Mycobacterium tuberculosis complexIII and Mycobacterium smegmatis complexIV in the presence of Q203 RCSB Protein Data Bank 7E1W Zhou S Wang W Gao Y Gong H Rao Z 2021 Cryo-EM structure of hybrid respiratory supercomplex consisting of Mycobacterium tuberculosis complexIII and Mycobacterium smegmatis complexIV in presence of TB47 RCSB Protein Data Bank 7E1X

Techniques: Construct, Clone Assay, Software