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  • 93
    Alomone Labs galr3
    Receptor specificity for SPX regulation of hypothalamic expression of orexigenic factors and their receptors. IP injection of SPX (5 nmol/mouse) was performed in mice with/without the co-treatment of the GalR2 antagonist M871 (50 nmol/mouse) or <t>GalR3</t> antagonist SNAP (50 nmol/mouse). The hypothalamus was harvested one hour later after IP injection. Total RNA was isolated and subjected to real-time PCR for transcript expression of NPY (A) , AgRP (B) , NPY5R (C) and GHSR (D) , respectively with β actin mRNA as internal control. The groups denoted by different letters represent a significant difference at p
    Galr3, supplied by Alomone Labs, used in various techniques. Bioz Stars score: 93/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/galr3/product/Alomone Labs
    Average 93 stars, based on 1 article reviews
    Price from $9.99 to $1999.99
    galr3 - by Bioz Stars, 2022-11
    93/100 stars
      Buy from Supplier

    93
    GeneTex anti galr3 rabbit polyclonal antibody
    Receptor specificity for SPX regulation of hypothalamic expression of orexigenic factors and their receptors. IP injection of SPX (5 nmol/mouse) was performed in mice with/without the co-treatment of the GalR2 antagonist M871 (50 nmol/mouse) or <t>GalR3</t> antagonist SNAP (50 nmol/mouse). The hypothalamus was harvested one hour later after IP injection. Total RNA was isolated and subjected to real-time PCR for transcript expression of NPY (A) , AgRP (B) , NPY5R (C) and GHSR (D) , respectively with β actin mRNA as internal control. The groups denoted by different letters represent a significant difference at p
    Anti Galr3 Rabbit Polyclonal Antibody, supplied by GeneTex, used in various techniques. Bioz Stars score: 93/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/anti galr3 rabbit polyclonal antibody/product/GeneTex
    Average 93 stars, based on 1 article reviews
    Price from $9.99 to $1999.99
    anti galr3 rabbit polyclonal antibody - by Bioz Stars, 2022-11
    93/100 stars
      Buy from Supplier

    93
    Alomone Labs anti galr3 extracellular antibody
    Receptor specificity for SPX regulation of hypothalamic expression of orexigenic factors and their receptors. IP injection of SPX (5 nmol/mouse) was performed in mice with/without the co-treatment of the GalR2 antagonist M871 (50 nmol/mouse) or <t>GalR3</t> antagonist SNAP (50 nmol/mouse). The hypothalamus was harvested one hour later after IP injection. Total RNA was isolated and subjected to real-time PCR for transcript expression of NPY (A) , AgRP (B) , NPY5R (C) and GHSR (D) , respectively with β actin mRNA as internal control. The groups denoted by different letters represent a significant difference at p
    Anti Galr3 Extracellular Antibody, supplied by Alomone Labs, used in various techniques. Bioz Stars score: 93/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/anti galr3 extracellular antibody/product/Alomone Labs
    Average 93 stars, based on 1 article reviews
    Price from $9.99 to $1999.99
    anti galr3 extracellular antibody - by Bioz Stars, 2022-11
    93/100 stars
      Buy from Supplier

    91
    Aviva Systems rabbit anti galr3
    Spexin inhibited bile acid synthesis through GALR2 and <t>GALR3</t> receptors. (A) Expression of spexin, GALR2 and GALR2 in hepatocytes characterized by confocal imaging. To investigate the role of GALR2/3 in spexin-regulated bile acid synthesis, the mice were i.p . injected with GALR2/3 antagonists 15 min before spexin treatment. One hour after drug treatment, serum and liver samples from each mouse were collected for further analysis. Control ( i.p . with saline); Spexin ( i.p . with spexin 300 μg/kg); M871 ( i.p . with M871 1000 μg/kg); Spexin+M871 ( i.p . with M871 15 mins before spexin treatment); SNAP37889 ( i.p . with SNAP37889 1 mg/kg); Spexin+SNAP37889 ( i.p . with SNAP37889 15 min before spexin treatment). Hepatic level of TBA (B) , hepatic expression of CYP7A1 (C) and serum concentration of C4 (D) were measured as described. Results are shown as Mean ± SEM ( n = 10/group). * p
    Rabbit Anti Galr3, supplied by Aviva Systems, used in various techniques. Bioz Stars score: 91/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/rabbit anti galr3/product/Aviva Systems
    Average 91 stars, based on 1 article reviews
    Price from $9.99 to $1999.99
    rabbit anti galr3 - by Bioz Stars, 2022-11
    91/100 stars
      Buy from Supplier

    Image Search Results


    Receptor specificity for SPX regulation of hypothalamic expression of orexigenic factors and their receptors. IP injection of SPX (5 nmol/mouse) was performed in mice with/without the co-treatment of the GalR2 antagonist M871 (50 nmol/mouse) or GalR3 antagonist SNAP (50 nmol/mouse). The hypothalamus was harvested one hour later after IP injection. Total RNA was isolated and subjected to real-time PCR for transcript expression of NPY (A) , AgRP (B) , NPY5R (C) and GHSR (D) , respectively with β actin mRNA as internal control. The groups denoted by different letters represent a significant difference at p

    Journal: Frontiers in Endocrinology

    Article Title: Mouse Spexin: (II) Functional Role as a Satiety Factor inhibiting Food Intake by Regulatory Actions Within the Hypothalamus

    doi: 10.3389/fendo.2021.681647

    Figure Lengend Snippet: Receptor specificity for SPX regulation of hypothalamic expression of orexigenic factors and their receptors. IP injection of SPX (5 nmol/mouse) was performed in mice with/without the co-treatment of the GalR2 antagonist M871 (50 nmol/mouse) or GalR3 antagonist SNAP (50 nmol/mouse). The hypothalamus was harvested one hour later after IP injection. Total RNA was isolated and subjected to real-time PCR for transcript expression of NPY (A) , AgRP (B) , NPY5R (C) and GHSR (D) , respectively with β actin mRNA as internal control. The groups denoted by different letters represent a significant difference at p

    Article Snippet: In previous studies, SPX was shown to activate GalR2 and GalR3 but not GalR1 expressed in HEK293 cells ( ).

    Techniques: Expressing, Injection, Mouse Assay, Isolation, Real-time Polymerase Chain Reaction

    Receptor specificity for SPX inhibition on food consumption in mice. (A) RT-PCR for GalR2 and GalR3 expression in the liver, brain and hypothalamus. Total RNA isolated from target tissues was used for RT-PCR with primers for GalR2 and GalR3, respectively, with GAPDH as internal control and PCR with no template as negative control (“-ve Ctrl”). (B) Western blot of GalR2 and GalR3 expression in the brain and hypothalamus. Tissue lysate was prepared from mouse brain and hypothalamus and subjected to SDS- PAGE and Western blot using th e antibody (Ab) for GalR2 and GalR3, respectively, with β actin as loading control. To confirm the specificity of immunoblotting, Western blot was also conducted with antibodies pre-absorbed with the synthetic peptides for GalR2/3 used to raise the antibody provided by the company. (C) GalR2 and GalR3 blockade on the inhibitory effect of SPX on food intake in mice. IP injection of SPX (5 nmol/mouse) was performed at 10:00 am with/without co-treatment of the GalR2 antagonist M871 (50 nmol/mouse) or GalR3 antagonist SNAP (50 nmol/mouse) to test the effect on food consumption occurred during the first hour in dark phase. (D) Effect of GalR2 activation on food consumption in mice. In parallel experiment, IP injection of the GalR2 agonist dN1-Qu (10 nmol/mouse) was conducted with SPX treatment (10 nmol/mouse) as positive control. For the data presented, the groups denoted by different letters represent a significant difference at p

    Journal: Frontiers in Endocrinology

    Article Title: Mouse Spexin: (II) Functional Role as a Satiety Factor inhibiting Food Intake by Regulatory Actions Within the Hypothalamus

    doi: 10.3389/fendo.2021.681647

    Figure Lengend Snippet: Receptor specificity for SPX inhibition on food consumption in mice. (A) RT-PCR for GalR2 and GalR3 expression in the liver, brain and hypothalamus. Total RNA isolated from target tissues was used for RT-PCR with primers for GalR2 and GalR3, respectively, with GAPDH as internal control and PCR with no template as negative control (“-ve Ctrl”). (B) Western blot of GalR2 and GalR3 expression in the brain and hypothalamus. Tissue lysate was prepared from mouse brain and hypothalamus and subjected to SDS- PAGE and Western blot using th e antibody (Ab) for GalR2 and GalR3, respectively, with β actin as loading control. To confirm the specificity of immunoblotting, Western blot was also conducted with antibodies pre-absorbed with the synthetic peptides for GalR2/3 used to raise the antibody provided by the company. (C) GalR2 and GalR3 blockade on the inhibitory effect of SPX on food intake in mice. IP injection of SPX (5 nmol/mouse) was performed at 10:00 am with/without co-treatment of the GalR2 antagonist M871 (50 nmol/mouse) or GalR3 antagonist SNAP (50 nmol/mouse) to test the effect on food consumption occurred during the first hour in dark phase. (D) Effect of GalR2 activation on food consumption in mice. In parallel experiment, IP injection of the GalR2 agonist dN1-Qu (10 nmol/mouse) was conducted with SPX treatment (10 nmol/mouse) as positive control. For the data presented, the groups denoted by different letters represent a significant difference at p

    Article Snippet: In previous studies, SPX was shown to activate GalR2 and GalR3 but not GalR1 expressed in HEK293 cells ( ).

    Techniques: Inhibition, Mouse Assay, Reverse Transcription Polymerase Chain Reaction, Expressing, Isolation, Polymerase Chain Reaction, Negative Control, Western Blot, SDS Page, Injection, Activation Assay, Positive Control

    Spexin inhibited bile acid synthesis through GALR2 and GALR3 receptors. (A) Expression of spexin, GALR2 and GALR2 in hepatocytes characterized by confocal imaging. To investigate the role of GALR2/3 in spexin-regulated bile acid synthesis, the mice were i.p . injected with GALR2/3 antagonists 15 min before spexin treatment. One hour after drug treatment, serum and liver samples from each mouse were collected for further analysis. Control ( i.p . with saline); Spexin ( i.p . with spexin 300 μg/kg); M871 ( i.p . with M871 1000 μg/kg); Spexin+M871 ( i.p . with M871 15 mins before spexin treatment); SNAP37889 ( i.p . with SNAP37889 1 mg/kg); Spexin+SNAP37889 ( i.p . with SNAP37889 15 min before spexin treatment). Hepatic level of TBA (B) , hepatic expression of CYP7A1 (C) and serum concentration of C4 (D) were measured as described. Results are shown as Mean ± SEM ( n = 10/group). * p

    Journal: Frontiers in Physiology

    Article Title: Spexin Acts as Novel Regulator for Bile Acid Synthesis

    doi: 10.3389/fphys.2018.00378

    Figure Lengend Snippet: Spexin inhibited bile acid synthesis through GALR2 and GALR3 receptors. (A) Expression of spexin, GALR2 and GALR2 in hepatocytes characterized by confocal imaging. To investigate the role of GALR2/3 in spexin-regulated bile acid synthesis, the mice were i.p . injected with GALR2/3 antagonists 15 min before spexin treatment. One hour after drug treatment, serum and liver samples from each mouse were collected for further analysis. Control ( i.p . with saline); Spexin ( i.p . with spexin 300 μg/kg); M871 ( i.p . with M871 1000 μg/kg); Spexin+M871 ( i.p . with M871 15 mins before spexin treatment); SNAP37889 ( i.p . with SNAP37889 1 mg/kg); Spexin+SNAP37889 ( i.p . with SNAP37889 15 min before spexin treatment). Hepatic level of TBA (B) , hepatic expression of CYP7A1 (C) and serum concentration of C4 (D) were measured as described. Results are shown as Mean ± SEM ( n = 10/group). * p

    Article Snippet: Primary and secondary antibodies used: rabbit anti-spexin (Bachem, T-4858, 1:500), rabbit anti-GALR2 (Aviva Systems Biology, OABF00517, 1:100), rabbit anti-GALR3 (Aviva Systems Biology, OAAF04899, 1:100), AlexaFluor 594-conjugated goat anti-rabbit IgG (Thermofisher Scientific, R37117, 1:500).

    Techniques: Expressing, Imaging, Mouse Assay, Injection, Concentration Assay