galactosidase-treated iga Search Results


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  • 94
    New England Biolabs galactosidase
    Galactosidase, supplied by New England Biolabs, used in various techniques. Bioz Stars score: 94/100, based on 27 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/galactosidase/product/New England Biolabs
    Average 94 stars, based on 27 article reviews
    Price from $9.99 to $1999.99
    galactosidase - by Bioz Stars, 2020-08
    94/100 stars
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    94
    Millipore α galactosidase
    α Galactosidase, supplied by Millipore, used in various techniques. Bioz Stars score: 94/100, based on 182 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/α galactosidase/product/Millipore
    Average 94 stars, based on 182 article reviews
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    88
    Boehringer Mannheim β d galactosidase
    β D Galactosidase, supplied by Boehringer Mannheim, used in various techniques. Bioz Stars score: 88/100, based on 5 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/β d galactosidase/product/Boehringer Mannheim
    Average 88 stars, based on 5 article reviews
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    95
    Millipore β gal
    Effect of HCV core on B and T lymphocyte functions. (a) HCV core on B and T lymphocyte function. Peripheral blood mononuclear cells (PBMC) were stimulated with PMA and calcium ionophore or phytohaemagglutinin (PHA) in the presence of <t>β-gal-core</t> or β-gal at 37º for 24 hr. The intracellular IFN-γ production in CD8 + T cells or IgM/IgG production on the surface of B cells was analysed by fluorescence-activated cell sorting (FACS) as described in Methods . (b) The effect of HCV core on the expression of co-stimulatory molecules and chemokine receptors on the surface of B lymphocytes. 1 × 10 6 PBMC were activated with PHA in the presence of β-gal-core or β-gal at 37º for 24 hr. PE–anti-CD86, PE–anti-CD154, or PE–anti-CD195 and fluorescein isothiocyanate (FITC)–anti-CD20 conjugate were used to examine the expression of these molecules on the surface of treated CD20 + B cells by FACS analysis.
    β Gal, supplied by Millipore, used in various techniques. Bioz Stars score: 95/100, based on 639 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    β gal - by Bioz Stars, 2020-08
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    99
    Millipore recombinant streptococcus pneumoniae β1 4 galactosidase
    Effect of HCV core on B and T lymphocyte functions. (a) HCV core on B and T lymphocyte function. Peripheral blood mononuclear cells (PBMC) were stimulated with PMA and calcium ionophore or phytohaemagglutinin (PHA) in the presence of <t>β-gal-core</t> or β-gal at 37º for 24 hr. The intracellular IFN-γ production in CD8 + T cells or IgM/IgG production on the surface of B cells was analysed by fluorescence-activated cell sorting (FACS) as described in Methods . (b) The effect of HCV core on the expression of co-stimulatory molecules and chemokine receptors on the surface of B lymphocytes. 1 × 10 6 PBMC were activated with PHA in the presence of β-gal-core or β-gal at 37º for 24 hr. PE–anti-CD86, PE–anti-CD154, or PE–anti-CD195 and fluorescein isothiocyanate (FITC)–anti-CD20 conjugate were used to examine the expression of these molecules on the surface of treated CD20 + B cells by FACS analysis.
    Recombinant Streptococcus Pneumoniae β1 4 Galactosidase, supplied by Millipore, used in various techniques. Bioz Stars score: 99/100, based on 6 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    Average 99 stars, based on 6 article reviews
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    96
    Promega anti β galactosidase
    Effect of HCV core on B and T lymphocyte functions. (a) HCV core on B and T lymphocyte function. Peripheral blood mononuclear cells (PBMC) were stimulated with PMA and calcium ionophore or phytohaemagglutinin (PHA) in the presence of <t>β-gal-core</t> or β-gal at 37º for 24 hr. The intracellular IFN-γ production in CD8 + T cells or IgM/IgG production on the surface of B cells was analysed by fluorescence-activated cell sorting (FACS) as described in Methods . (b) The effect of HCV core on the expression of co-stimulatory molecules and chemokine receptors on the surface of B lymphocytes. 1 × 10 6 PBMC were activated with PHA in the presence of β-gal-core or β-gal at 37º for 24 hr. PE–anti-CD86, PE–anti-CD154, or PE–anti-CD195 and fluorescein isothiocyanate (FITC)–anti-CD20 conjugate were used to examine the expression of these molecules on the surface of treated CD20 + B cells by FACS analysis.
    Anti β Galactosidase, supplied by Promega, used in various techniques. Bioz Stars score: 96/100, based on 377 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    85
    Bethyl iga elisa kit
    The <t>ELISA</t> of total (A, C, D) and HMW (B, E) adiponectin after stimulation of HMCs with native or deSial/deGal IgA1. In HMCs, native <t>IgA</t> upregulated the total (A) and high molecular weight (HMW) (B) adiponectin release to the supernatant in a dose- (A, B) and time-dependent manner (C). Native IgA also increased the total and HMW adiponectin concentration in cell lysates in a dose-dependent manner (D, E). HMCs were cultured with different concentrations of native or deSial/deGal IgA1 (0, 6, 12.5, 25 or 50 µg/ml) for 48 h. For the time course study, HMCs were cultured with 25 µg/ml of native or deSial/deGal IgA1 for 0, 1, 6, 24 or 48 h. The concentrations of adiponectin were expressed as the means ± SE. Open diamonds, native IgA; closed circles, deSial/deGal IgA1. * P = 0.05 vs. medium control; # P = 0.01, native IgA vs. deSial/deGal IgA1.
    Iga Elisa Kit, supplied by Bethyl, used in various techniques. Bioz Stars score: 85/100, based on 24 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    92
    Millipore anti β galactosidase antibody
    The <t>ELISA</t> of total (A, C, D) and HMW (B, E) adiponectin after stimulation of HMCs with native or deSial/deGal IgA1. In HMCs, native <t>IgA</t> upregulated the total (A) and high molecular weight (HMW) (B) adiponectin release to the supernatant in a dose- (A, B) and time-dependent manner (C). Native IgA also increased the total and HMW adiponectin concentration in cell lysates in a dose-dependent manner (D, E). HMCs were cultured with different concentrations of native or deSial/deGal IgA1 (0, 6, 12.5, 25 or 50 µg/ml) for 48 h. For the time course study, HMCs were cultured with 25 µg/ml of native or deSial/deGal IgA1 for 0, 1, 6, 24 or 48 h. The concentrations of adiponectin were expressed as the means ± SE. Open diamonds, native IgA; closed circles, deSial/deGal IgA1. * P = 0.05 vs. medium control; # P = 0.01, native IgA vs. deSial/deGal IgA1.
    Anti β Galactosidase Antibody, supplied by Millipore, used in various techniques. Bioz Stars score: 92/100, based on 75 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    Average 92 stars, based on 75 article reviews
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    anti β galactosidase antibody - by Bioz Stars, 2020-08
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    92
    Seikagaku β galactosidase
    The <t>ELISA</t> of total (A, C, D) and HMW (B, E) adiponectin after stimulation of HMCs with native or deSial/deGal IgA1. In HMCs, native <t>IgA</t> upregulated the total (A) and high molecular weight (HMW) (B) adiponectin release to the supernatant in a dose- (A, B) and time-dependent manner (C). Native IgA also increased the total and HMW adiponectin concentration in cell lysates in a dose-dependent manner (D, E). HMCs were cultured with different concentrations of native or deSial/deGal IgA1 (0, 6, 12.5, 25 or 50 µg/ml) for 48 h. For the time course study, HMCs were cultured with 25 µg/ml of native or deSial/deGal IgA1 for 0, 1, 6, 24 or 48 h. The concentrations of adiponectin were expressed as the means ± SE. Open diamonds, native IgA; closed circles, deSial/deGal IgA1. * P = 0.05 vs. medium control; # P = 0.01, native IgA vs. deSial/deGal IgA1.
    β Galactosidase, supplied by Seikagaku, used in various techniques. Bioz Stars score: 92/100, based on 27 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    92
    ProZyme β galactosidase
    The <t>ELISA</t> of total (A, C, D) and HMW (B, E) adiponectin after stimulation of HMCs with native or deSial/deGal IgA1. In HMCs, native <t>IgA</t> upregulated the total (A) and high molecular weight (HMW) (B) adiponectin release to the supernatant in a dose- (A, B) and time-dependent manner (C). Native IgA also increased the total and HMW adiponectin concentration in cell lysates in a dose-dependent manner (D, E). HMCs were cultured with different concentrations of native or deSial/deGal IgA1 (0, 6, 12.5, 25 or 50 µg/ml) for 48 h. For the time course study, HMCs were cultured with 25 µg/ml of native or deSial/deGal IgA1 for 0, 1, 6, 24 or 48 h. The concentrations of adiponectin were expressed as the means ± SE. Open diamonds, native IgA; closed circles, deSial/deGal IgA1. * P = 0.05 vs. medium control; # P = 0.01, native IgA vs. deSial/deGal IgA1.
    β Galactosidase, supplied by ProZyme, used in various techniques. Bioz Stars score: 92/100, based on 79 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    89
    Seikagaku endo β galactosidase
    The <t>ELISA</t> of total (A, C, D) and HMW (B, E) adiponectin after stimulation of HMCs with native or deSial/deGal IgA1. In HMCs, native <t>IgA</t> upregulated the total (A) and high molecular weight (HMW) (B) adiponectin release to the supernatant in a dose- (A, B) and time-dependent manner (C). Native IgA also increased the total and HMW adiponectin concentration in cell lysates in a dose-dependent manner (D, E). HMCs were cultured with different concentrations of native or deSial/deGal IgA1 (0, 6, 12.5, 25 or 50 µg/ml) for 48 h. For the time course study, HMCs were cultured with 25 µg/ml of native or deSial/deGal IgA1 for 0, 1, 6, 24 or 48 h. The concentrations of adiponectin were expressed as the means ± SE. Open diamonds, native IgA; closed circles, deSial/deGal IgA1. * P = 0.05 vs. medium control; # P = 0.01, native IgA vs. deSial/deGal IgA1.
    Endo β Galactosidase, supplied by Seikagaku, used in various techniques. Bioz Stars score: 89/100, based on 62 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    95
    SouthernBiotech goat anti human iga
    The <t>ELISA</t> of total (A, C, D) and HMW (B, E) adiponectin after stimulation of HMCs with native or deSial/deGal IgA1. In HMCs, native <t>IgA</t> upregulated the total (A) and high molecular weight (HMW) (B) adiponectin release to the supernatant in a dose- (A, B) and time-dependent manner (C). Native IgA also increased the total and HMW adiponectin concentration in cell lysates in a dose-dependent manner (D, E). HMCs were cultured with different concentrations of native or deSial/deGal IgA1 (0, 6, 12.5, 25 or 50 µg/ml) for 48 h. For the time course study, HMCs were cultured with 25 µg/ml of native or deSial/deGal IgA1 for 0, 1, 6, 24 or 48 h. The concentrations of adiponectin were expressed as the means ± SE. Open diamonds, native IgA; closed circles, deSial/deGal IgA1. * P = 0.05 vs. medium control; # P = 0.01, native IgA vs. deSial/deGal IgA1.
    Goat Anti Human Iga, supplied by SouthernBiotech, used in various techniques. Bioz Stars score: 95/100, based on 89 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/goat anti human iga/product/SouthernBiotech
    Average 95 stars, based on 89 article reviews
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    90
    US Biological Life Sciences anti β galactosidase β gal antibody
    The <t>ELISA</t> of total (A, C, D) and HMW (B, E) adiponectin after stimulation of HMCs with native or deSial/deGal IgA1. In HMCs, native <t>IgA</t> upregulated the total (A) and high molecular weight (HMW) (B) adiponectin release to the supernatant in a dose- (A, B) and time-dependent manner (C). Native IgA also increased the total and HMW adiponectin concentration in cell lysates in a dose-dependent manner (D, E). HMCs were cultured with different concentrations of native or deSial/deGal IgA1 (0, 6, 12.5, 25 or 50 µg/ml) for 48 h. For the time course study, HMCs were cultured with 25 µg/ml of native or deSial/deGal IgA1 for 0, 1, 6, 24 or 48 h. The concentrations of adiponectin were expressed as the means ± SE. Open diamonds, native IgA; closed circles, deSial/deGal IgA1. * P = 0.05 vs. medium control; # P = 0.01, native IgA vs. deSial/deGal IgA1.
    Anti β Galactosidase β Gal Antibody, supplied by US Biological Life Sciences, used in various techniques. Bioz Stars score: 90/100, based on 9 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    80
    Thermo Fisher mouse anti β galactosidase igg1 monoclonal antibody
    The <t>ELISA</t> of total (A, C, D) and HMW (B, E) adiponectin after stimulation of HMCs with native or deSial/deGal IgA1. In HMCs, native <t>IgA</t> upregulated the total (A) and high molecular weight (HMW) (B) adiponectin release to the supernatant in a dose- (A, B) and time-dependent manner (C). Native IgA also increased the total and HMW adiponectin concentration in cell lysates in a dose-dependent manner (D, E). HMCs were cultured with different concentrations of native or deSial/deGal IgA1 (0, 6, 12.5, 25 or 50 µg/ml) for 48 h. For the time course study, HMCs were cultured with 25 µg/ml of native or deSial/deGal IgA1 for 0, 1, 6, 24 or 48 h. The concentrations of adiponectin were expressed as the means ± SE. Open diamonds, native IgA; closed circles, deSial/deGal IgA1. * P = 0.05 vs. medium control; # P = 0.01, native IgA vs. deSial/deGal IgA1.
    Mouse Anti β Galactosidase Igg1 Monoclonal Antibody, supplied by Thermo Fisher, used in various techniques. Bioz Stars score: 80/100, based on 5 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/mouse anti β galactosidase igg1 monoclonal antibody/product/Thermo Fisher
    Average 80 stars, based on 5 article reviews
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    85
    Bio-Rad goat anti murine polyclonal igg β galactosidase
    The <t>ELISA</t> of total (A, C, D) and HMW (B, E) adiponectin after stimulation of HMCs with native or deSial/deGal IgA1. In HMCs, native <t>IgA</t> upregulated the total (A) and high molecular weight (HMW) (B) adiponectin release to the supernatant in a dose- (A, B) and time-dependent manner (C). Native IgA also increased the total and HMW adiponectin concentration in cell lysates in a dose-dependent manner (D, E). HMCs were cultured with different concentrations of native or deSial/deGal IgA1 (0, 6, 12.5, 25 or 50 µg/ml) for 48 h. For the time course study, HMCs were cultured with 25 µg/ml of native or deSial/deGal IgA1 for 0, 1, 6, 24 or 48 h. The concentrations of adiponectin were expressed as the means ± SE. Open diamonds, native IgA; closed circles, deSial/deGal IgA1. * P = 0.05 vs. medium control; # P = 0.01, native IgA vs. deSial/deGal IgA1.
    Goat Anti Murine Polyclonal Igg β Galactosidase, supplied by Bio-Rad, used in various techniques. Bioz Stars score: 85/100, based on 7 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/goat anti murine polyclonal igg β galactosidase/product/Bio-Rad
    Average 85 stars, based on 7 article reviews
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    85
    ProZyme β n acetylhexosaminidase treated
    The <t>ELISA</t> of total (A, C, D) and HMW (B, E) adiponectin after stimulation of HMCs with native or deSial/deGal IgA1. In HMCs, native <t>IgA</t> upregulated the total (A) and high molecular weight (HMW) (B) adiponectin release to the supernatant in a dose- (A, B) and time-dependent manner (C). Native IgA also increased the total and HMW adiponectin concentration in cell lysates in a dose-dependent manner (D, E). HMCs were cultured with different concentrations of native or deSial/deGal IgA1 (0, 6, 12.5, 25 or 50 µg/ml) for 48 h. For the time course study, HMCs were cultured with 25 µg/ml of native or deSial/deGal IgA1 for 0, 1, 6, 24 or 48 h. The concentrations of adiponectin were expressed as the means ± SE. Open diamonds, native IgA; closed circles, deSial/deGal IgA1. * P = 0.05 vs. medium control; # P = 0.01, native IgA vs. deSial/deGal IgA1.
    β N Acetylhexosaminidase Treated, supplied by ProZyme, used in various techniques. Bioz Stars score: 85/100, based on 2 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    90
    Santa Cruz Biotechnology non immune mouse immunoglobulin
    The <t>ELISA</t> of total (A, C, D) and HMW (B, E) adiponectin after stimulation of HMCs with native or deSial/deGal IgA1. In HMCs, native <t>IgA</t> upregulated the total (A) and high molecular weight (HMW) (B) adiponectin release to the supernatant in a dose- (A, B) and time-dependent manner (C). Native IgA also increased the total and HMW adiponectin concentration in cell lysates in a dose-dependent manner (D, E). HMCs were cultured with different concentrations of native or deSial/deGal IgA1 (0, 6, 12.5, 25 or 50 µg/ml) for 48 h. For the time course study, HMCs were cultured with 25 µg/ml of native or deSial/deGal IgA1 for 0, 1, 6, 24 or 48 h. The concentrations of adiponectin were expressed as the means ± SE. Open diamonds, native IgA; closed circles, deSial/deGal IgA1. * P = 0.05 vs. medium control; # P = 0.01, native IgA vs. deSial/deGal IgA1.
    Non Immune Mouse Immunoglobulin, supplied by Santa Cruz Biotechnology, used in various techniques. Bioz Stars score: 90/100, based on 9 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/non immune mouse immunoglobulin/product/Santa Cruz Biotechnology
    Average 90 stars, based on 9 article reviews
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    88
    GE Healthcare donkey anti rabbit immunoglobulin g
    The <t>ELISA</t> of total (A, C, D) and HMW (B, E) adiponectin after stimulation of HMCs with native or deSial/deGal IgA1. In HMCs, native <t>IgA</t> upregulated the total (A) and high molecular weight (HMW) (B) adiponectin release to the supernatant in a dose- (A, B) and time-dependent manner (C). Native IgA also increased the total and HMW adiponectin concentration in cell lysates in a dose-dependent manner (D, E). HMCs were cultured with different concentrations of native or deSial/deGal IgA1 (0, 6, 12.5, 25 or 50 µg/ml) for 48 h. For the time course study, HMCs were cultured with 25 µg/ml of native or deSial/deGal IgA1 for 0, 1, 6, 24 or 48 h. The concentrations of adiponectin were expressed as the means ± SE. Open diamonds, native IgA; closed circles, deSial/deGal IgA1. * P = 0.05 vs. medium control; # P = 0.01, native IgA vs. deSial/deGal IgA1.
    Donkey Anti Rabbit Immunoglobulin G, supplied by GE Healthcare, used in various techniques. Bioz Stars score: 88/100, based on 51 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    80
    Thermo Fisher biotin labeled rabbit anti mouse immunoglobulin g1
    The <t>ELISA</t> of total (A, C, D) and HMW (B, E) adiponectin after stimulation of HMCs with native or deSial/deGal IgA1. In HMCs, native <t>IgA</t> upregulated the total (A) and high molecular weight (HMW) (B) adiponectin release to the supernatant in a dose- (A, B) and time-dependent manner (C). Native IgA also increased the total and HMW adiponectin concentration in cell lysates in a dose-dependent manner (D, E). HMCs were cultured with different concentrations of native or deSial/deGal IgA1 (0, 6, 12.5, 25 or 50 µg/ml) for 48 h. For the time course study, HMCs were cultured with 25 µg/ml of native or deSial/deGal IgA1 for 0, 1, 6, 24 or 48 h. The concentrations of adiponectin were expressed as the means ± SE. Open diamonds, native IgA; closed circles, deSial/deGal IgA1. * P = 0.05 vs. medium control; # P = 0.01, native IgA vs. deSial/deGal IgA1.
    Biotin Labeled Rabbit Anti Mouse Immunoglobulin G1, supplied by Thermo Fisher, used in various techniques. Bioz Stars score: 80/100, based on 6 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    88
    Thermo Fisher fluorescein isothiocyanate conjugated goat anti mouse immunoglobulin g igg
    The <t>ELISA</t> of total (A, C, D) and HMW (B, E) adiponectin after stimulation of HMCs with native or deSial/deGal IgA1. In HMCs, native <t>IgA</t> upregulated the total (A) and high molecular weight (HMW) (B) adiponectin release to the supernatant in a dose- (A, B) and time-dependent manner (C). Native IgA also increased the total and HMW adiponectin concentration in cell lysates in a dose-dependent manner (D, E). HMCs were cultured with different concentrations of native or deSial/deGal IgA1 (0, 6, 12.5, 25 or 50 µg/ml) for 48 h. For the time course study, HMCs were cultured with 25 µg/ml of native or deSial/deGal IgA1 for 0, 1, 6, 24 or 48 h. The concentrations of adiponectin were expressed as the means ± SE. Open diamonds, native IgA; closed circles, deSial/deGal IgA1. * P = 0.05 vs. medium control; # P = 0.01, native IgA vs. deSial/deGal IgA1.
    Fluorescein Isothiocyanate Conjugated Goat Anti Mouse Immunoglobulin G Igg, supplied by Thermo Fisher, used in various techniques. Bioz Stars score: 88/100, based on 27 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/fluorescein isothiocyanate conjugated goat anti mouse immunoglobulin g igg/product/Thermo Fisher
    Average 88 stars, based on 27 article reviews
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    90
    Millipore neurminidase
    The <t>ELISA</t> of total (A, C, D) and HMW (B, E) adiponectin after stimulation of HMCs with native or deSial/deGal IgA1. In HMCs, native <t>IgA</t> upregulated the total (A) and high molecular weight (HMW) (B) adiponectin release to the supernatant in a dose- (A, B) and time-dependent manner (C). Native IgA also increased the total and HMW adiponectin concentration in cell lysates in a dose-dependent manner (D, E). HMCs were cultured with different concentrations of native or deSial/deGal IgA1 (0, 6, 12.5, 25 or 50 µg/ml) for 48 h. For the time course study, HMCs were cultured with 25 µg/ml of native or deSial/deGal IgA1 for 0, 1, 6, 24 or 48 h. The concentrations of adiponectin were expressed as the means ± SE. Open diamonds, native IgA; closed circles, deSial/deGal IgA1. * P = 0.05 vs. medium control; # P = 0.01, native IgA vs. deSial/deGal IgA1.
    Neurminidase, supplied by Millipore, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/neurminidase/product/Millipore
    Average 90 stars, based on 1 article reviews
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    92
    Roche neuraminidase
    The <t>ELISA</t> of total (A, C, D) and HMW (B, E) adiponectin after stimulation of HMCs with native or deSial/deGal IgA1. In HMCs, native <t>IgA</t> upregulated the total (A) and high molecular weight (HMW) (B) adiponectin release to the supernatant in a dose- (A, B) and time-dependent manner (C). Native IgA also increased the total and HMW adiponectin concentration in cell lysates in a dose-dependent manner (D, E). HMCs were cultured with different concentrations of native or deSial/deGal IgA1 (0, 6, 12.5, 25 or 50 µg/ml) for 48 h. For the time course study, HMCs were cultured with 25 µg/ml of native or deSial/deGal IgA1 for 0, 1, 6, 24 or 48 h. The concentrations of adiponectin were expressed as the means ± SE. Open diamonds, native IgA; closed circles, deSial/deGal IgA1. * P = 0.05 vs. medium control; # P = 0.01, native IgA vs. deSial/deGal IgA1.
    Neuraminidase, supplied by Roche, used in various techniques. Bioz Stars score: 92/100, based on 445 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    92
    Shire Plc agalsidase alfa
    Serum <t>anti-agalsidase</t> IgG titers at baseline. Titers of antibodies to agalsidase alfa and agalsidase beta at baseline were not significantly different even in patients who had not yet been exposed to agalsidase beta (patients 1, 3, 5, 8, 13, and 14).
    Agalsidase Alfa, supplied by Shire Plc, used in various techniques. Bioz Stars score: 92/100, based on 945 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    99
    Millipore rabbit polyclonal igg anti galactosylceramidase
    Serum <t>anti-agalsidase</t> IgG titers at baseline. Titers of antibodies to agalsidase alfa and agalsidase beta at baseline were not significantly different even in patients who had not yet been exposed to agalsidase beta (patients 1, 3, 5, 8, 13, and 14).
    Rabbit Polyclonal Igg Anti Galactosylceramidase, supplied by Millipore, used in various techniques. Bioz Stars score: 99/100, based on 36 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    Image Search Results


    Effect of HCV core on B and T lymphocyte functions. (a) HCV core on B and T lymphocyte function. Peripheral blood mononuclear cells (PBMC) were stimulated with PMA and calcium ionophore or phytohaemagglutinin (PHA) in the presence of β-gal-core or β-gal at 37º for 24 hr. The intracellular IFN-γ production in CD8 + T cells or IgM/IgG production on the surface of B cells was analysed by fluorescence-activated cell sorting (FACS) as described in Methods . (b) The effect of HCV core on the expression of co-stimulatory molecules and chemokine receptors on the surface of B lymphocytes. 1 × 10 6 PBMC were activated with PHA in the presence of β-gal-core or β-gal at 37º for 24 hr. PE–anti-CD86, PE–anti-CD154, or PE–anti-CD195 and fluorescein isothiocyanate (FITC)–anti-CD20 conjugate were used to examine the expression of these molecules on the surface of treated CD20 + B cells by FACS analysis.

    Journal: Immunology

    Article Title: Differential regulation of SOCS-1 signalling in B and T lymphocytes by hepatitis C virus core protein

    doi: 10.1111/j.1365-2567.2008.02829.x

    Figure Lengend Snippet: Effect of HCV core on B and T lymphocyte functions. (a) HCV core on B and T lymphocyte function. Peripheral blood mononuclear cells (PBMC) were stimulated with PMA and calcium ionophore or phytohaemagglutinin (PHA) in the presence of β-gal-core or β-gal at 37º for 24 hr. The intracellular IFN-γ production in CD8 + T cells or IgM/IgG production on the surface of B cells was analysed by fluorescence-activated cell sorting (FACS) as described in Methods . (b) The effect of HCV core on the expression of co-stimulatory molecules and chemokine receptors on the surface of B lymphocytes. 1 × 10 6 PBMC were activated with PHA in the presence of β-gal-core or β-gal at 37º for 24 hr. PE–anti-CD86, PE–anti-CD154, or PE–anti-CD195 and fluorescein isothiocyanate (FITC)–anti-CD20 conjugate were used to examine the expression of these molecules on the surface of treated CD20 + B cells by FACS analysis.

    Article Snippet: To further characterize the specificity of the effect of core protein on CD69 expression on resting B cells, MACS-purified CD20+ B lymphocytes were treated with either HCV core (2 μg/ml, ViroGen), HCV NS3 (2 μg/ml, ViroGen), β-gal (2 μg/ml, Sigma), or C1q (2 μg/ml; Sigma) for 24 hr, and CD69 expression was measured as above.

    Techniques: Fluorescence, FACS, Expressing

    Differential regulation of B and T lymphocyte activation by HCV core protein. (a) CD69 expression on activated CD20 + B cells and CD4 + T cells in the presence of core. PBMC were activated with 5 μg/ml of phytohaemagglutinin (PHA) in the presence or absence of 2 μg/ml of core protein at 37º for 24 hr in a 5% CO 2 atmosphere. CD69 expression on CD20 + B cells (upper panel) and CD4 + T cells (lower panel) was determined by fluorescence-activated cell sorting (FACS) with PE-conjugated anti-CD69 antibody and either fluorescein isothiocyanate (FITC)-conjugated anti-CD4 or FITC-conjugated anti-CD20 mcAb. The percentages of CD20 + and CD4 + lymphocytes positive for CD69 are shown. The results were reproducible in two independent experiments using different donors. (b) Abrogation of core-mediated differential regulation of B and T lymphocyte activation by α-gC1qR antibody. PHA-activated PBMC were treated with 2 μg/ml of HCV core in the presence of either 1:10 (volume/volume) α-gC1qR or control Ab at 37º for 24 h. CD69 expression on CD20 + B cells and CD4 + T cells was examined and shown as above. (c) Specificity of CD69 upregulation by HCV core. Magnetic antibody cell sorting (MACS)-purified CD20 + B lymphocytes were treated with either HCV core (2 μg/ml), HCV NS3 (2 μg/ml), β-gal (2 μg/ml), or C1q (2 μg/ml) for 24 hr, and CD69 expression was measured as above.

    Journal: Immunology

    Article Title: Differential regulation of SOCS-1 signalling in B and T lymphocytes by hepatitis C virus core protein

    doi: 10.1111/j.1365-2567.2008.02829.x

    Figure Lengend Snippet: Differential regulation of B and T lymphocyte activation by HCV core protein. (a) CD69 expression on activated CD20 + B cells and CD4 + T cells in the presence of core. PBMC were activated with 5 μg/ml of phytohaemagglutinin (PHA) in the presence or absence of 2 μg/ml of core protein at 37º for 24 hr in a 5% CO 2 atmosphere. CD69 expression on CD20 + B cells (upper panel) and CD4 + T cells (lower panel) was determined by fluorescence-activated cell sorting (FACS) with PE-conjugated anti-CD69 antibody and either fluorescein isothiocyanate (FITC)-conjugated anti-CD4 or FITC-conjugated anti-CD20 mcAb. The percentages of CD20 + and CD4 + lymphocytes positive for CD69 are shown. The results were reproducible in two independent experiments using different donors. (b) Abrogation of core-mediated differential regulation of B and T lymphocyte activation by α-gC1qR antibody. PHA-activated PBMC were treated with 2 μg/ml of HCV core in the presence of either 1:10 (volume/volume) α-gC1qR or control Ab at 37º for 24 h. CD69 expression on CD20 + B cells and CD4 + T cells was examined and shown as above. (c) Specificity of CD69 upregulation by HCV core. Magnetic antibody cell sorting (MACS)-purified CD20 + B lymphocytes were treated with either HCV core (2 μg/ml), HCV NS3 (2 μg/ml), β-gal (2 μg/ml), or C1q (2 μg/ml) for 24 hr, and CD69 expression was measured as above.

    Article Snippet: To further characterize the specificity of the effect of core protein on CD69 expression on resting B cells, MACS-purified CD20+ B lymphocytes were treated with either HCV core (2 μg/ml, ViroGen), HCV NS3 (2 μg/ml, ViroGen), β-gal (2 μg/ml, Sigma), or C1q (2 μg/ml; Sigma) for 24 hr, and CD69 expression was measured as above.

    Techniques: Activation Assay, Expressing, Fluorescence, FACS, Magnetic Cell Separation, Purification

    Differential regulation of suppressor of cytokine signalling-1 (SOCS-1) and signal transducer and activator of transcription-1 (STAT1) in B and T lymphocytes by HCV core. (a) gC1qR is involved in HCV core-induced induction of SOCS-1 gene expression in TCR-stimulated peripheral blood mononuclear cells (PBMCs). Left panel, up-regulation of SOCS-1 in anti-CD3/CD28-stimulated T cells by HCV core. PBMC were incubated with 1 μg/ml of anti-CD3/CD28 in the presence or absence of 2 μg/ml of β-gal-core for 24 hr, and SOCS-1 and β-actin genes were amplified as described in Materials and Methods . The fold changes of the amplified genes in cells treated with or without core were quantitatively analyzed by real-time reverse transcription–polymerase chain reaction (RT-PCR) and shown below, with the absence of core treatment deemed as one after normalization by β-actin reference control. *represents P

    Journal: Immunology

    Article Title: Differential regulation of SOCS-1 signalling in B and T lymphocytes by hepatitis C virus core protein

    doi: 10.1111/j.1365-2567.2008.02829.x

    Figure Lengend Snippet: Differential regulation of suppressor of cytokine signalling-1 (SOCS-1) and signal transducer and activator of transcription-1 (STAT1) in B and T lymphocytes by HCV core. (a) gC1qR is involved in HCV core-induced induction of SOCS-1 gene expression in TCR-stimulated peripheral blood mononuclear cells (PBMCs). Left panel, up-regulation of SOCS-1 in anti-CD3/CD28-stimulated T cells by HCV core. PBMC were incubated with 1 μg/ml of anti-CD3/CD28 in the presence or absence of 2 μg/ml of β-gal-core for 24 hr, and SOCS-1 and β-actin genes were amplified as described in Materials and Methods . The fold changes of the amplified genes in cells treated with or without core were quantitatively analyzed by real-time reverse transcription–polymerase chain reaction (RT-PCR) and shown below, with the absence of core treatment deemed as one after normalization by β-actin reference control. *represents P

    Article Snippet: To further characterize the specificity of the effect of core protein on CD69 expression on resting B cells, MACS-purified CD20+ B lymphocytes were treated with either HCV core (2 μg/ml, ViroGen), HCV NS3 (2 μg/ml, ViroGen), β-gal (2 μg/ml, Sigma), or C1q (2 μg/ml; Sigma) for 24 hr, and CD69 expression was measured as above.

    Techniques: Expressing, Incubation, Amplification, Reverse Transcription Polymerase Chain Reaction

    HCV core promotes B cell proliferation. 1 × 10 6 magnetic antibody cell sorting (MACS)-purified and carboxy-fluorescein diacetate succinimidyl ester (CFSE)-labeled CD20 + B cells were stimulated with PHA (5 μg/ml) and IL-2 (50 μg/ml) in the presence of β-gal (2 μg/ml) or HCV core (2 μg/ml) at 37º, 5% CO 2 for 5 days. Data were collected by flow cytometer and represented as histograms. Percentages of distinct cell division peaks were gated as M1, M2, and M3. During each round of cell division, the relative intensity of the fluorescent dye is decreased by half.

    Journal: Immunology

    Article Title: Differential regulation of SOCS-1 signalling in B and T lymphocytes by hepatitis C virus core protein

    doi: 10.1111/j.1365-2567.2008.02829.x

    Figure Lengend Snippet: HCV core promotes B cell proliferation. 1 × 10 6 magnetic antibody cell sorting (MACS)-purified and carboxy-fluorescein diacetate succinimidyl ester (CFSE)-labeled CD20 + B cells were stimulated with PHA (5 μg/ml) and IL-2 (50 μg/ml) in the presence of β-gal (2 μg/ml) or HCV core (2 μg/ml) at 37º, 5% CO 2 for 5 days. Data were collected by flow cytometer and represented as histograms. Percentages of distinct cell division peaks were gated as M1, M2, and M3. During each round of cell division, the relative intensity of the fluorescent dye is decreased by half.

    Article Snippet: To further characterize the specificity of the effect of core protein on CD69 expression on resting B cells, MACS-purified CD20+ B lymphocytes were treated with either HCV core (2 μg/ml, ViroGen), HCV NS3 (2 μg/ml, ViroGen), β-gal (2 μg/ml, Sigma), or C1q (2 μg/ml; Sigma) for 24 hr, and CD69 expression was measured as above.

    Techniques: FACS, Magnetic Cell Separation, Purification, Labeling, Flow Cytometry, Cytometry

    The ELISA of total (A, C, D) and HMW (B, E) adiponectin after stimulation of HMCs with native or deSial/deGal IgA1. In HMCs, native IgA upregulated the total (A) and high molecular weight (HMW) (B) adiponectin release to the supernatant in a dose- (A, B) and time-dependent manner (C). Native IgA also increased the total and HMW adiponectin concentration in cell lysates in a dose-dependent manner (D, E). HMCs were cultured with different concentrations of native or deSial/deGal IgA1 (0, 6, 12.5, 25 or 50 µg/ml) for 48 h. For the time course study, HMCs were cultured with 25 µg/ml of native or deSial/deGal IgA1 for 0, 1, 6, 24 or 48 h. The concentrations of adiponectin were expressed as the means ± SE. Open diamonds, native IgA; closed circles, deSial/deGal IgA1. * P = 0.05 vs. medium control; # P = 0.01, native IgA vs. deSial/deGal IgA1.

    Journal: PLoS ONE

    Article Title: Suppression of Adiponectin by Aberrantly Glycosylated IgA1 in Glomerular Mesangial Cells In Vitro and In Vivo

    doi: 10.1371/journal.pone.0033965

    Figure Lengend Snippet: The ELISA of total (A, C, D) and HMW (B, E) adiponectin after stimulation of HMCs with native or deSial/deGal IgA1. In HMCs, native IgA upregulated the total (A) and high molecular weight (HMW) (B) adiponectin release to the supernatant in a dose- (A, B) and time-dependent manner (C). Native IgA also increased the total and HMW adiponectin concentration in cell lysates in a dose-dependent manner (D, E). HMCs were cultured with different concentrations of native or deSial/deGal IgA1 (0, 6, 12.5, 25 or 50 µg/ml) for 48 h. For the time course study, HMCs were cultured with 25 µg/ml of native or deSial/deGal IgA1 for 0, 1, 6, 24 or 48 h. The concentrations of adiponectin were expressed as the means ± SE. Open diamonds, native IgA; closed circles, deSial/deGal IgA1. * P = 0.05 vs. medium control; # P = 0.01, native IgA vs. deSial/deGal IgA1.

    Article Snippet: The samples, including non-treated IgA (native IgA) and neuraminidase- and galactosidase-treated IgA, were added to each well, incubated overnight, and diluted to achieve comparable levels of IgA (50 µg/ml) as determined using the IgA ELISA kit (Bethyl Laboratories, Montgomery, TX, USA).

    Techniques: Enzyme-linked Immunosorbent Assay, Molecular Weight, Concentration Assay, Cell Culture

    Serum anti-agalsidase IgG titers at baseline. Titers of antibodies to agalsidase alfa and agalsidase beta at baseline were not significantly different even in patients who had not yet been exposed to agalsidase beta (patients 1, 3, 5, 8, 13, and 14).

    Journal: JIMD Reports

    Article Title: Reduction of Plasma Globotriaosylsphingosine Levels After Switching from Agalsidase Alfa to Agalsidase Beta as Enzyme Replacement Therapy for Fabry Disease

    doi: 10.1007/8904_2015_483

    Figure Lengend Snippet: Serum anti-agalsidase IgG titers at baseline. Titers of antibodies to agalsidase alfa and agalsidase beta at baseline were not significantly different even in patients who had not yet been exposed to agalsidase beta (patients 1, 3, 5, 8, 13, and 14).

    Article Snippet: There have been no randomized, double-blind trials of patients matched for sex, age, and disease severity to allow a direct comparison of the effectiveness of agalsidase beta and agalsidase alfa at their approved doses (Desnick and Schuchman ).

    Techniques:

    Patient-level plasma lyso-GL-3 concentrations according to Clinical visit after the switch to agalsidase beta at 1.0 mg/kg every other week. Figure includes three of the four patients (patients 2, 3, 8, and 11) who had the highest agalsidase antibody

    Journal: JIMD Reports

    Article Title: Reduction of Plasma Globotriaosylsphingosine Levels After Switching from Agalsidase Alfa to Agalsidase Beta as Enzyme Replacement Therapy for Fabry Disease

    doi: 10.1007/8904_2015_483

    Figure Lengend Snippet: Patient-level plasma lyso-GL-3 concentrations according to Clinical visit after the switch to agalsidase beta at 1.0 mg/kg every other week. Figure includes three of the four patients (patients 2, 3, 8, and 11) who had the highest agalsidase antibody

    Article Snippet: There have been no randomized, double-blind trials of patients matched for sex, age, and disease severity to allow a direct comparison of the effectiveness of agalsidase beta and agalsidase alfa at their approved doses (Desnick and Schuchman ).

    Techniques:

    Patient-level urine GL-3 concentrations according to clinical visit after the switch to agalsidase beta at 1.0 mg/kg every other week. GL - 3 globotriaosylceramide

    Journal: JIMD Reports

    Article Title: Reduction of Plasma Globotriaosylsphingosine Levels After Switching from Agalsidase Alfa to Agalsidase Beta as Enzyme Replacement Therapy for Fabry Disease

    doi: 10.1007/8904_2015_483

    Figure Lengend Snippet: Patient-level urine GL-3 concentrations according to clinical visit after the switch to agalsidase beta at 1.0 mg/kg every other week. GL - 3 globotriaosylceramide

    Article Snippet: There have been no randomized, double-blind trials of patients matched for sex, age, and disease severity to allow a direct comparison of the effectiveness of agalsidase beta and agalsidase alfa at their approved doses (Desnick and Schuchman ).

    Techniques:

    Patient-level plasma GL-3 concentrations according to Clinical visit after the switch to agalsidase beta at 1.0 mg/kg every other week. For two patients (patients 12 and 13), baseline and month 2 values were not available. GL - 3 globotriaosylceramide

    Journal: JIMD Reports

    Article Title: Reduction of Plasma Globotriaosylsphingosine Levels After Switching from Agalsidase Alfa to Agalsidase Beta as Enzyme Replacement Therapy for Fabry Disease

    doi: 10.1007/8904_2015_483

    Figure Lengend Snippet: Patient-level plasma GL-3 concentrations according to Clinical visit after the switch to agalsidase beta at 1.0 mg/kg every other week. For two patients (patients 12 and 13), baseline and month 2 values were not available. GL - 3 globotriaosylceramide

    Article Snippet: There have been no randomized, double-blind trials of patients matched for sex, age, and disease severity to allow a direct comparison of the effectiveness of agalsidase beta and agalsidase alfa at their approved doses (Desnick and Schuchman ).

    Techniques: