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    Agilent technologies agilent technologies g2565ca scanner
    Agilent Technologies G2565ca Scanner, supplied by Agilent technologies, used in various techniques. Bioz Stars score: 86/100, based on 14 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    Agilent technologies g2565aa g2565ab scanner
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    Agilent technologies g2565 aa microarray scanner
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    Agilent technologies g2565aa g2565ba microarray scanner system
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    Agilent technologies g2565ba microarray scanner system
    Experimental procedures used to identify oscillating and herbivore-induced metabolites and their associated genes in different tissues of Nicotiana attenuata . (A) Wild type (WT) N. attenuata plants were harvested every 4 h for two days during the initiation of stem elongation. To mimic herbivory, oral secretions (OS) of the larvae of the specialist herbivore, M. sexta , were immediately applied to puncture wounds made in leaves at 1 pm. Water treatment of puncture wounds on separate plants was used to distinguish OS-specific from wound-induced changes in metabolites and transcripts. (B) Metabolites from three different tissues, source leaves, sink leaves, and roots of N. attenuata were isolated. The leaf at node 0 had completed the sink to source transition and the leaf at node +1 was older by one leaf position than the leaf at node 0 and so forth. Source leaves (at nodes +2, +1, 0) were wounded with a fabric pattern wheel and treated with 20 µl of M. sexta OS, which was diluted 1∶5 with water. Untreated leaves (at nodes −1, −2) and roots were harvested to monitor systemic responses. (C) After sample preparation from six biological replicates, a 40% methanol extraction method optimized for the defense metabolites of N. attenuata was used and the metabolites separated with a rapid separation liquid chromatography (RSLC) on a C 18 column and detected by ESI-TOF-MS (electrospray ionization time-of-flight mass spectrometer) for parents and their daughter ions. Peak picking and alignments were performed with the XCMS package [19] . Diurnal oscillating metabolites were extracted by the pattern matching algorithms of HAYSTACK tool [20] . In-house and public databases were used to identify oscillating metabolites and a 44K <t>Agilent</t> <t>microarray</t> designed for N. attenuata was used to examine the expression of metabolite-related genes.
    G2565ba Microarray Scanner System, supplied by Agilent technologies, used in various techniques. Bioz Stars score: 91/100, based on 3066 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    Agilent technologies g2565ca microarray scanner system
    Experimental procedures used to identify oscillating and herbivore-induced metabolites and their associated genes in different tissues of Nicotiana attenuata . (A) Wild type (WT) N. attenuata plants were harvested every 4 h for two days during the initiation of stem elongation. To mimic herbivory, oral secretions (OS) of the larvae of the specialist herbivore, M. sexta , were immediately applied to puncture wounds made in leaves at 1 pm. Water treatment of puncture wounds on separate plants was used to distinguish OS-specific from wound-induced changes in metabolites and transcripts. (B) Metabolites from three different tissues, source leaves, sink leaves, and roots of N. attenuata were isolated. The leaf at node 0 had completed the sink to source transition and the leaf at node +1 was older by one leaf position than the leaf at node 0 and so forth. Source leaves (at nodes +2, +1, 0) were wounded with a fabric pattern wheel and treated with 20 µl of M. sexta OS, which was diluted 1∶5 with water. Untreated leaves (at nodes −1, −2) and roots were harvested to monitor systemic responses. (C) After sample preparation from six biological replicates, a 40% methanol extraction method optimized for the defense metabolites of N. attenuata was used and the metabolites separated with a rapid separation liquid chromatography (RSLC) on a C 18 column and detected by ESI-TOF-MS (electrospray ionization time-of-flight mass spectrometer) for parents and their daughter ions. Peak picking and alignments were performed with the XCMS package [19] . Diurnal oscillating metabolites were extracted by the pattern matching algorithms of HAYSTACK tool [20] . In-house and public databases were used to identify oscillating metabolites and a 44K <t>Agilent</t> <t>microarray</t> designed for N. attenuata was used to examine the expression of metabolite-related genes.
    G2565ca Microarray Scanner System, supplied by Agilent technologies, used in various techniques. Bioz Stars score: 91/100, based on 578 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    Agilent technologies microarray g2565ba fluorescent scanner
    Experimental procedures used to identify oscillating and herbivore-induced metabolites and their associated genes in different tissues of Nicotiana attenuata . (A) Wild type (WT) N. attenuata plants were harvested every 4 h for two days during the initiation of stem elongation. To mimic herbivory, oral secretions (OS) of the larvae of the specialist herbivore, M. sexta , were immediately applied to puncture wounds made in leaves at 1 pm. Water treatment of puncture wounds on separate plants was used to distinguish OS-specific from wound-induced changes in metabolites and transcripts. (B) Metabolites from three different tissues, source leaves, sink leaves, and roots of N. attenuata were isolated. The leaf at node 0 had completed the sink to source transition and the leaf at node +1 was older by one leaf position than the leaf at node 0 and so forth. Source leaves (at nodes +2, +1, 0) were wounded with a fabric pattern wheel and treated with 20 µl of M. sexta OS, which was diluted 1∶5 with water. Untreated leaves (at nodes −1, −2) and roots were harvested to monitor systemic responses. (C) After sample preparation from six biological replicates, a 40% methanol extraction method optimized for the defense metabolites of N. attenuata was used and the metabolites separated with a rapid separation liquid chromatography (RSLC) on a C 18 column and detected by ESI-TOF-MS (electrospray ionization time-of-flight mass spectrometer) for parents and their daughter ions. Peak picking and alignments were performed with the XCMS package [19] . Diurnal oscillating metabolites were extracted by the pattern matching algorithms of HAYSTACK tool [20] . In-house and public databases were used to identify oscillating metabolites and a 44K <t>Agilent</t> <t>microarray</t> designed for N. attenuata was used to examine the expression of metabolite-related genes.
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    Agilent technologies laser scanner
    Experimental procedures used to identify oscillating and herbivore-induced metabolites and their associated genes in different tissues of Nicotiana attenuata . (A) Wild type (WT) N. attenuata plants were harvested every 4 h for two days during the initiation of stem elongation. To mimic herbivory, oral secretions (OS) of the larvae of the specialist herbivore, M. sexta , were immediately applied to puncture wounds made in leaves at 1 pm. Water treatment of puncture wounds on separate plants was used to distinguish OS-specific from wound-induced changes in metabolites and transcripts. (B) Metabolites from three different tissues, source leaves, sink leaves, and roots of N. attenuata were isolated. The leaf at node 0 had completed the sink to source transition and the leaf at node +1 was older by one leaf position than the leaf at node 0 and so forth. Source leaves (at nodes +2, +1, 0) were wounded with a fabric pattern wheel and treated with 20 µl of M. sexta OS, which was diluted 1∶5 with water. Untreated leaves (at nodes −1, −2) and roots were harvested to monitor systemic responses. (C) After sample preparation from six biological replicates, a 40% methanol extraction method optimized for the defense metabolites of N. attenuata was used and the metabolites separated with a rapid separation liquid chromatography (RSLC) on a C 18 column and detected by ESI-TOF-MS (electrospray ionization time-of-flight mass spectrometer) for parents and their daughter ions. Peak picking and alignments were performed with the XCMS package [19] . Diurnal oscillating metabolites were extracted by the pattern matching algorithms of HAYSTACK tool [20] . In-house and public databases were used to identify oscillating metabolites and a 44K <t>Agilent</t> <t>microarray</t> designed for N. attenuata was used to examine the expression of metabolite-related genes.
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    Experimental procedures used to identify oscillating and herbivore-induced metabolites and their associated genes in different tissues of Nicotiana attenuata . (A) Wild type (WT) N. attenuata plants were harvested every 4 h for two days during the initiation of stem elongation. To mimic herbivory, oral secretions (OS) of the larvae of the specialist herbivore, M. sexta , were immediately applied to puncture wounds made in leaves at 1 pm. Water treatment of puncture wounds on separate plants was used to distinguish OS-specific from wound-induced changes in metabolites and transcripts. (B) Metabolites from three different tissues, source leaves, sink leaves, and roots of N. attenuata were isolated. The leaf at node 0 had completed the sink to source transition and the leaf at node +1 was older by one leaf position than the leaf at node 0 and so forth. Source leaves (at nodes +2, +1, 0) were wounded with a fabric pattern wheel and treated with 20 µl of M. sexta OS, which was diluted 1∶5 with water. Untreated leaves (at nodes −1, −2) and roots were harvested to monitor systemic responses. (C) After sample preparation from six biological replicates, a 40% methanol extraction method optimized for the defense metabolites of N. attenuata was used and the metabolites separated with a rapid separation liquid chromatography (RSLC) on a C 18 column and detected by ESI-TOF-MS (electrospray ionization time-of-flight mass spectrometer) for parents and their daughter ions. Peak picking and alignments were performed with the XCMS package [19] . Diurnal oscillating metabolites were extracted by the pattern matching algorithms of HAYSTACK tool [20] . In-house and public databases were used to identify oscillating metabolites and a 44K Agilent microarray designed for N. attenuata was used to examine the expression of metabolite-related genes.

    Journal: PLoS ONE

    Article Title: Tissue Specific Diurnal Rhythms of Metabolites and Their Regulation during Herbivore Attack in a Native Tobacco, Nicotiana attenuata

    doi: 10.1371/journal.pone.0026214

    Figure Lengend Snippet: Experimental procedures used to identify oscillating and herbivore-induced metabolites and their associated genes in different tissues of Nicotiana attenuata . (A) Wild type (WT) N. attenuata plants were harvested every 4 h for two days during the initiation of stem elongation. To mimic herbivory, oral secretions (OS) of the larvae of the specialist herbivore, M. sexta , were immediately applied to puncture wounds made in leaves at 1 pm. Water treatment of puncture wounds on separate plants was used to distinguish OS-specific from wound-induced changes in metabolites and transcripts. (B) Metabolites from three different tissues, source leaves, sink leaves, and roots of N. attenuata were isolated. The leaf at node 0 had completed the sink to source transition and the leaf at node +1 was older by one leaf position than the leaf at node 0 and so forth. Source leaves (at nodes +2, +1, 0) were wounded with a fabric pattern wheel and treated with 20 µl of M. sexta OS, which was diluted 1∶5 with water. Untreated leaves (at nodes −1, −2) and roots were harvested to monitor systemic responses. (C) After sample preparation from six biological replicates, a 40% methanol extraction method optimized for the defense metabolites of N. attenuata was used and the metabolites separated with a rapid separation liquid chromatography (RSLC) on a C 18 column and detected by ESI-TOF-MS (electrospray ionization time-of-flight mass spectrometer) for parents and their daughter ions. Peak picking and alignments were performed with the XCMS package [19] . Diurnal oscillating metabolites were extracted by the pattern matching algorithms of HAYSTACK tool [20] . In-house and public databases were used to identify oscillating metabolites and a 44K Agilent microarray designed for N. attenuata was used to examine the expression of metabolite-related genes.

    Article Snippet: Agilent microarray scanner (G2565BA) and Scan Control software were used to obtain intensity of the spots.

    Techniques: Isolation, Sample Prep, Liquid Chromatography, Mass Spectrometry, Microarray, Expressing

    NF-κB signaling analysis in THP-1 cells infected with SFTSV. The IPA platform was used to analyze components involved in the NF-κB response and their relationships and interactions. Fold changes of transcript expression levels of the genes in NF-κB signaling at 18 hpi were imported from the Agilent microarray analysis described above. Fold changes are shown in red (upregulation) and green (downregulation).

    Journal: Journal of Virology

    Article Title: Suppression of the Interferon and NF-?B Responses by Severe Fever with Thrombocytopenia Syndrome Virus

    doi: 10.1128/JVI.00612-12

    Figure Lengend Snippet: NF-κB signaling analysis in THP-1 cells infected with SFTSV. The IPA platform was used to analyze components involved in the NF-κB response and their relationships and interactions. Fold changes of transcript expression levels of the genes in NF-κB signaling at 18 hpi were imported from the Agilent microarray analysis described above. Fold changes are shown in red (upregulation) and green (downregulation).

    Article Snippet: Human 4x44K slides (Agilent) were used for hybridization, followed by scanning with an Agilent scanner (G2565BA).

    Techniques: Infection, Indirect Immunoperoxidase Assay, Expressing, Microarray

    Pathway analysis of the antiviral IFN response in infected THP-1 cells. The IPA platform was used to analyze components of the IFN response involved in both IRF and NF-κB signaling. Fold changes of transcript expression levels of the genes in IRF and NF-κB signaling at 18 hpi were imported from the Agilent microarray analysis. Fold changes are shown in red (upregulation) and green (downregulation).

    Journal: Journal of Virology

    Article Title: Suppression of the Interferon and NF-?B Responses by Severe Fever with Thrombocytopenia Syndrome Virus

    doi: 10.1128/JVI.00612-12

    Figure Lengend Snippet: Pathway analysis of the antiviral IFN response in infected THP-1 cells. The IPA platform was used to analyze components of the IFN response involved in both IRF and NF-κB signaling. Fold changes of transcript expression levels of the genes in IRF and NF-κB signaling at 18 hpi were imported from the Agilent microarray analysis. Fold changes are shown in red (upregulation) and green (downregulation).

    Article Snippet: Human 4x44K slides (Agilent) were used for hybridization, followed by scanning with an Agilent scanner (G2565BA).

    Techniques: Infection, Indirect Immunoperoxidase Assay, Expressing, Microarray

    Sox21 is a master regulator of normal keratinization in the cuticle of the hair shaft. ( A ) Summary of comparative microarray analyses of P5 back skin samples from WT, heterozygous, and homozygous mice. Gene ontology and expression profiling of the 119

    Journal: Proceedings of the National Academy of Sciences of the United States of America

    Article Title: The disruption of Sox21-mediated hair shaft cuticle differentiation causes cyclic alopecia in mice

    doi: 10.1073/pnas.0808324106

    Figure Lengend Snippet: Sox21 is a master regulator of normal keratinization in the cuticle of the hair shaft. ( A ) Summary of comparative microarray analyses of P5 back skin samples from WT, heterozygous, and homozygous mice. Gene ontology and expression profiling of the 119

    Article Snippet: Arrays were scanned with a microarray scanner system (G2565BA; Agilent) and the data were analyzed with Genespring GX software, version 7.3.1 (Agilent).

    Techniques: Microarray, Mouse Assay, Expressing